CN112136835A - Compound microbial agent capable of improving stress resistance of crops - Google Patents
Compound microbial agent capable of improving stress resistance of crops Download PDFInfo
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Abstract
The invention discloses a compound microbial agent capable of improving stress resistance of crops, which is prepared from the following raw materials in parts by weight: compound microorganism bacteria, trace elements, a drying agent and diatomite; the compound microbial agent for improving stress resistance of crops is prepared from the following raw materials in parts by weight: 15-25 parts of compound microorganism bacteria, 5-10 parts of trace elements, 3-7 parts of drying agent and 2-4 parts of diatomite; the microorganism of the invention has reasonable collocation of types, adopts the combination of bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis, has strong complementarity, and the compound microbial inoculum can be rapidly propagated after being used to form an advantageous population, thereby stimulating the growth and development of crops, improving the stress resistance of the crops, enhancing the cold and drought resistance and the pest and disease resistance of the crops, fixing nitrogen, dissolving phosphorus and potassium, increasing soil nutrients, improving soil structure, improving fertilizer utilization rate, increasing both production and income.
Description
Technical Field
The invention relates to the field of plant antibacterial agents, in particular to a compound microbial agent capable of improving stress resistance of crops.
Background
The microbial agent, especially the compound microbial agent composed of various microorganisms, is a biotechnology which has wide application prospect in recent years. The microbial inoculum can not only restore the soil polluted by petroleum and degrade organic pollutants in water, but also prevent and treat plant diseases and insect pests, promote crop production and increase both production and income, some microbial inoculants can improve the stress resistance of plants since the 70 th century, and some complex microbial inoculants are successively developed in countries or regions such as Europe, America, Japan and the like, and a lot of microbial inoculants begin to be produced in a large scale, and form serialized products. In the 80 th century, EM (effective microbiota) developed by professor of the university of yuzu, japan has been used widely in more than 90 countries relating to the field of plant cultivation and environmental purification, and has achieved significant economic and social benefits. The microbial inoculum is researched and popularized earlier in some developed countries, the related technology is mature, and the application is more common. In recent years, microbial agents that can cause great effects all over the world, such as EM, have not appeared abroad. The agricultural microbial agent is properly used, so that the yield of agricultural products can be increased, the quality of the agricultural products can be improved, the using amount of chemical fertilizers can be reduced, the cost can be reduced, the soil can be improved, and the ecological environment can be protected; however, the existing microbial agents are not ideal in the using process.
Compared with the research on related microbial agents in developed countries abroad, the research on the microbial agents starts from the 80 th generation of the 20 th century in China, and from theory to practice, certain achievements are obtained successively from the utilization of a single strain to the compound application of a plurality of strains. The cold damage at low temperature is one of the main meteorological disasters in China, and is most frequent and serious in northern areas. The low-temperature cold injury is agricultural natural disaster which causes yield reduction because the daily average temperature is lower than the lower limit index of the proper temperature for the growth and development of crops, and influences the growth and development or fructification of crops in the period of crop growth and development, wherein the long-time continuous low-temperature weather occurs although the daily minimum temperature is more than 0 ℃ and the weather is warmer, or the short-term strong low-temperature weather process occurs in the period of crop reproductive growth. The cold damage at low temperature affects the growth and normal physiological and biochemical processes of plants to different degrees.
Disclosure of Invention
The invention aims to provide a compound microbial agent capable of improving stress resistance of crops, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a compound microbial agent capable of improving stress resistance of crops is prepared from the following raw materials in parts by weight: compound microbe, trace elements, desiccant and diatomite.
Preferably, the compound microbial agent for improving stress resistance of crops is prepared from the following raw materials in parts by weight: 15-25 parts of compound microorganism bacteria, 5-10 parts of trace elements, 3-7 parts of drying agent and 2-4 parts of diatomite.
Preferably, the compound microbial agent for improving stress resistance of crops is prepared from the following raw materials in parts by weight: 10 parts of compound microbial strains, 8 parts of trace elements, 5 parts of a drying agent and 3 parts of diatomite.
Preferably, the compound microorganism bacteria comprise bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis, and the mass ratio of the bacillus polymyxa, the bacillus laterosporus, the trichoderma viride, the bacillus megaterium, the lactobacillus plantarum and the bacillus licheniformis is 3: 1: 2: 3: 1.
Preferably, the trace elements consist of zinc sulfate, magnesium sulfate and borax, and the mass ratio of the zinc sulfate to the magnesium sulfate to the borax is 1: 2.
Preferably, the drying agent is one of bentonite, silica gel, white carbon black or attapulgite.
A preparation method of a compound microbial agent capable of improving stress resistance of crops comprises the following steps:
s1: preparing materials: and weighing the composite microbial bacteria, the trace elements, the drying agent and the diatomite according to the proportion.
S2: fermenting strains: and (2) respectively inoculating the composite microorganisms weighed in the step (S1) into an activation culture medium at the temperature of 22-25 ℃ under a closed condition, performing activation culture for 3-5 days, then respectively performing fermentation propagation on the cultured strains in a production fermentation culture medium for 2-3 days, and continuously shaking or stirring in the processes of the activation culture and the fermentation propagation to respectively obtain bacterial liquids of bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis.
S3: crushing the trace elements and the drying agent: and (3) respectively crushing the drying agent and the trace elements weighed in the step (1) to obtain crushed dry trace elements and drying agent.
S4: drying bacterial liquid: respectively adsorbing the bacterial liquids of the bacillus polymyxa, the bacillus laterosporus, the trichoderma viride, the bacillus megaterium, the lactobacillus plantarum and the bacillus licheniformis obtained in the step S2 by using diatomite carriers, drying and crushing to obtain various bacterial powders, and mixing the bacterial powders;
s5: mixing: and (4) uniformly mixing the drying agent and the trace elements which are crushed in the step (S3) and the step (S4) with the mixed bacterium powder to obtain the compound microbial agent.
Has the advantages that: the invention has the beneficial effects that: the microorganism of the invention has reasonable collocation of types, adopts the combination of bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis, has strong complementarity, and the compound microbial inoculum can be rapidly propagated after being used to form an advantageous population, thereby stimulating the growth and development of crops, improving the stress resistance of the crops, enhancing the cold and drought resistance and the pest and disease resistance of the crops, fixing nitrogen, dissolving phosphorus and potassium, increasing soil nutrients, improving soil structure, improving fertilizer utilization rate, increasing both production and income.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Example 1
The compound microbial agent capable of improving stress resistance of crops is prepared from the following raw materials in parts by weight: compound microbe, trace elements, desiccant and diatomite.
Preferably, the compound microbial agent for improving stress resistance of crops is prepared from the following raw materials in parts by weight: 15 parts of compound microorganism bacteria, 5 parts of trace elements, 3 parts of a drying agent and 2 parts of diatomite.
Preferably, the compound microorganism bacteria comprise bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis, and the mass ratio of the bacillus polymyxa, the bacillus laterosporus, the trichoderma viride, the bacillus megaterium, the lactobacillus plantarum and the bacillus licheniformis is 3: 1: 2: 3: 1.
Preferably, the trace elements consist of zinc sulfate, magnesium sulfate and borax, and the mass ratio of the zinc sulfate to the magnesium sulfate to the borax is 1: 2.
Preferably, the drying agent is one of bentonite, silica gel, white carbon black or attapulgite.
A preparation method of a compound microbial agent capable of improving stress resistance of crops comprises the following steps:
s1: preparing materials: and weighing the composite microbial bacteria, the trace elements, the drying agent and the diatomite according to the proportion.
S2: fermenting strains: and (2) respectively inoculating the composite microorganisms weighed in the step (S1) into an activation culture medium at the temperature of 22-25 ℃ under a closed condition, performing activation culture for 3-5 days, then respectively performing fermentation propagation on the cultured strains in a production fermentation culture medium for 2-3 days, and continuously shaking or stirring in the processes of the activation culture and the fermentation propagation to respectively obtain bacterial liquids of bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis.
S3: crushing the trace elements and the drying agent: and (3) respectively crushing the drying agent and the trace elements weighed in the step (1) to obtain crushed dry trace elements and drying agent.
S4: drying bacterial liquid: respectively adsorbing the bacterial liquids of the bacillus polymyxa, the bacillus laterosporus, the trichoderma viride, the bacillus megaterium, the lactobacillus plantarum and the bacillus licheniformis obtained in the step S2 by using diatomite carriers, drying and crushing to obtain various bacterial powders, and mixing the bacterial powders;
s5: mixing: and (4) uniformly mixing the drying agent and the trace elements which are crushed in the step (S3) and the step (S4) with the mixed bacterium powder to obtain the compound microbial agent.
Example 2
The compound microbial agent capable of improving stress resistance of crops is prepared from the following raw materials in parts by weight: compound microbe, trace elements, desiccant and diatomite.
Preferably, the compound microbial agent for improving stress resistance of crops is prepared from the following raw materials in parts by weight: 25 parts of compound microorganism bacteria, 10 parts of trace elements, 7 parts of a drying agent and 4 parts of diatomite.
Preferably, the compound microorganism bacteria comprise bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis, and the mass ratio of the bacillus polymyxa, the bacillus laterosporus, the trichoderma viride, the bacillus megaterium, the lactobacillus plantarum and the bacillus licheniformis is 3: 1: 2: 3: 1.
Preferably, the trace elements consist of zinc sulfate, magnesium sulfate and borax, and the mass ratio of the zinc sulfate to the magnesium sulfate to the borax is 1: 2.
Preferably, the drying agent is one of bentonite, silica gel, white carbon black or attapulgite.
A preparation method of a compound microbial agent capable of improving stress resistance of crops comprises the following steps:
s1: preparing materials: and weighing the composite microbial bacteria, the trace elements, the drying agent and the diatomite according to the proportion.
S2: fermenting strains: and (2) respectively inoculating the composite microorganisms weighed in the step (S1) into an activation culture medium at the temperature of 22-25 ℃ under a closed condition, performing activation culture for 3-5 days, then respectively performing fermentation propagation on the cultured strains in a production fermentation culture medium for 2-3 days, and continuously shaking or stirring in the processes of the activation culture and the fermentation propagation to respectively obtain bacterial liquids of bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis.
S3: crushing the trace elements and the drying agent: and (3) respectively crushing the drying agent and the trace elements weighed in the step (1) to obtain crushed dry trace elements and drying agent.
S4: drying bacterial liquid: respectively adsorbing the bacterial liquids of the bacillus polymyxa, the bacillus laterosporus, the trichoderma viride, the bacillus megaterium, the lactobacillus plantarum and the bacillus licheniformis obtained in the step S2 by using diatomite carriers, drying and crushing to obtain various bacterial powders, and mixing the bacterial powders;
s5: mixing: and (4) uniformly mixing the drying agent and the trace elements which are crushed in the step (S3) and the step (S4) with the mixed bacterium powder to obtain the compound microbial agent.
Example 3
The compound microbial agent capable of improving stress resistance of crops is prepared from the following raw materials in parts by weight: compound microbe, trace elements, desiccant and diatomite.
Preferably, the compound microbial agent for improving stress resistance of crops is prepared from the following raw materials in parts by weight: 10 parts of compound microbial strains, 8 parts of trace elements, 5 parts of a drying agent and 3 parts of diatomite.
Preferably, the compound microorganism bacteria comprise bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis, and the mass ratio of the bacillus polymyxa, the bacillus laterosporus, the trichoderma viride, the bacillus megaterium, the lactobacillus plantarum and the bacillus licheniformis is 3: 1: 2: 3: 1.
Preferably, the trace elements consist of zinc sulfate, magnesium sulfate and borax, and the mass ratio of the zinc sulfate to the magnesium sulfate to the borax is 1: 2.
Preferably, the drying agent is one of bentonite, silica gel, white carbon black or attapulgite.
A preparation method of a compound microbial agent capable of improving stress resistance of crops comprises the following steps:
s1: preparing materials: and weighing the composite microbial bacteria, the trace elements, the drying agent and the diatomite according to the proportion.
S2: fermenting strains: and (2) respectively inoculating the composite microorganisms weighed in the step (S1) into an activation culture medium at the temperature of 22-25 ℃ under a closed condition, performing activation culture for 3-5 days, then respectively performing fermentation propagation on the cultured strains in a production fermentation culture medium for 2-3 days, and continuously shaking or stirring in the processes of the activation culture and the fermentation propagation to respectively obtain bacterial liquids of bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis.
S3: crushing the trace elements and the drying agent: and (3) respectively crushing the drying agent and the trace elements weighed in the step (1) to obtain crushed dry trace elements and drying agent.
S4: drying bacterial liquid: respectively adsorbing the bacterial liquids of the bacillus polymyxa, the bacillus laterosporus, the trichoderma viride, the bacillus megaterium, the lactobacillus plantarum and the bacillus licheniformis obtained in the step S2 by using diatomite carriers, drying and crushing to obtain various bacterial powders, and mixing the bacterial powders;
s5: mixing: and (4) uniformly mixing the drying agent and the trace elements which are crushed in the step (S3) and the step (S4) with the mixed bacterium powder to obtain the compound microbial agent.
The microorganism of the invention has reasonable collocation of types, adopts the combination of bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis, has strong complementarity, and the compound microbial inoculum can be rapidly propagated after being used to form an advantageous population, thereby stimulating the growth and development of crops, improving the stress resistance of the crops, enhancing the cold and drought resistance and the pest and disease resistance of the crops, fixing nitrogen, dissolving phosphorus and potassium, increasing soil nutrients, improving soil structure, improving fertilizer utilization rate, increasing both production and income.
The embodiments described above are preferred embodiments of the present invention, and not all embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (7)
1. The compound microbial agent capable of improving stress resistance of crops is characterized by being prepared from the following raw materials in parts by weight: compound microbe, trace elements, desiccant and diatomite.
2. The compound microbial inoculant capable of improving stress resistance of crops as claimed in claim 1, which is prepared from the following raw materials in parts by weight: 15-25 parts of compound microorganism bacteria, 5-10 parts of trace elements, 3-7 parts of drying agent and 2-4 parts of diatomite.
3. The compound microbial inoculant capable of improving stress resistance of crops as claimed in claim 1, which is prepared from the following raw materials in parts by weight: 10 parts of compound microbial strains, 8 parts of trace elements, 5 parts of a drying agent and 3 parts of diatomite.
4. The compound microbial inoculant for improving stress tolerance of crops as claimed in any one of claims 1 to 3, wherein the compound microbial inoculant comprises bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis, and the mass ratio of the bacillus polymyxa, the bacillus laterosporus, the trichoderma viride, the bacillus megaterium, the lactobacillus plantarum and the bacillus licheniformis is 3: 1: 2: 3: 1.
5. The compound microbial inoculant for improving stress resistance of crops as claimed in any one of claims 1 to 3, wherein the microbial inoculant comprises a first microbial inoculant and a second microbial inoculant; the trace elements consist of zinc sulfate, magnesium sulfate and borax, and the mass ratio of the zinc sulfate to the magnesium sulfate to the borax is 1: 2.
6. The compound microbial inoculant for improving stress resistance of crops as claimed in any one of claims 1 to 3, wherein the microbial inoculant comprises a first microbial inoculant and a second microbial inoculant; the drying agent is one of bentonite, silica gel, white carbon black or attapulgite.
7. A preparation method of a compound microbial agent capable of improving stress resistance of crops is characterized by comprising the following steps:
s1: preparing materials: and weighing the composite microbial bacteria, the trace elements, the drying agent and the diatomite according to the proportion.
S2: fermenting strains: and (2) respectively inoculating the composite microorganisms weighed in the step (S1) into an activation culture medium at the temperature of 22-25 ℃ under a closed condition, performing activation culture for 3-5 days, then respectively performing fermentation propagation on the cultured strains in a production fermentation culture medium for 2-3 days, and continuously shaking or stirring in the processes of the activation culture and the fermentation propagation to respectively obtain bacterial liquids of bacillus polymyxa, bacillus laterosporus, trichoderma viride, bacillus megaterium, lactobacillus plantarum and bacillus licheniformis.
S3: crushing the trace elements and the drying agent: and (3) respectively crushing the drying agent and the trace elements weighed in the step (1) to obtain crushed dry trace elements and drying agent.
S4: drying bacterial liquid: respectively adsorbing the bacterial liquids of the bacillus polymyxa, the bacillus laterosporus, the trichoderma viride, the bacillus megaterium, the lactobacillus plantarum and the bacillus licheniformis obtained in the step S2 by using diatomite carriers, drying and crushing to obtain various bacterial powders, and mixing the bacterial powders;
s5: mixing: and (4) uniformly mixing the drying agent and the trace elements which are crushed in the step (S3) and the step (S4) with the mixed bacterium powder to obtain the compound microbial agent.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113717725A (en) * | 2021-10-13 | 2021-11-30 | 辽宁省农业科学院 | Microbial agent |
| CN115433044A (en) * | 2022-09-13 | 2022-12-06 | 云南大微时代生物科技有限公司 | Compound microbial preparation product and preparation method thereof |
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2020
- 2020-10-01 CN CN202011095043.4A patent/CN112136835A/en active Pending
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| CN109320345A (en) * | 2018-10-29 | 2019-02-12 | 上海市农业科学院 | It is a kind of for improving the complex micro organism fungicide and purposes of crop yield and quality |
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| 何若天等: "《农用益生菌生产与应用手册》", 28 February 2016, 金盾出版社 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113717725A (en) * | 2021-10-13 | 2021-11-30 | 辽宁省农业科学院 | Microbial agent |
| CN115433044A (en) * | 2022-09-13 | 2022-12-06 | 云南大微时代生物科技有限公司 | Compound microbial preparation product and preparation method thereof |
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Application publication date: 20201229 |