CN107937303A - A kind of method of extreme thermophilic cooperative fermentation production composite ultra-high temperature compost bacterium - Google Patents
A kind of method of extreme thermophilic cooperative fermentation production composite ultra-high temperature compost bacterium Download PDFInfo
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- CN107937303A CN107937303A CN201711103240.4A CN201711103240A CN107937303A CN 107937303 A CN107937303 A CN 107937303A CN 201711103240 A CN201711103240 A CN 201711103240A CN 107937303 A CN107937303 A CN 107937303A
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- 238000000855 fermentation Methods 0.000 title claims abstract description 71
- 230000004151 fermentation Effects 0.000 title claims abstract description 71
- 241000894006 Bacteria Species 0.000 title claims abstract description 58
- 238000000034 method Methods 0.000 title claims abstract description 29
- 239000002361 compost Substances 0.000 title claims abstract description 25
- 239000002131 composite material Substances 0.000 title claims abstract description 16
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 10
- 241000478152 Calditerricola yamamurae Species 0.000 claims abstract description 26
- 241000130178 Calditerricola satsumensis Species 0.000 claims abstract description 23
- 241000589499 Thermus thermophilus Species 0.000 claims abstract description 23
- 239000002609 medium Substances 0.000 claims abstract description 16
- 239000010802 sludge Substances 0.000 claims abstract description 15
- 239000001963 growth medium Substances 0.000 claims abstract description 9
- 239000002054 inoculum Substances 0.000 claims description 14
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- 235000015099 wheat brans Nutrition 0.000 claims description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical class [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 4
- 229930006000 Sucrose Natural products 0.000 claims description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 4
- 235000019270 ammonium chloride Nutrition 0.000 claims description 4
- 239000003610 charcoal Substances 0.000 claims description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 4
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical class [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- 239000005720 sucrose Substances 0.000 claims description 4
- 239000008107 starch Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000000969 carrier Substances 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 claims 1
- 230000012010 growth Effects 0.000 abstract description 6
- 230000006641 stabilisation Effects 0.000 abstract description 3
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- 239000003795 chemical substances by application Substances 0.000 abstract description 2
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- 230000000474 nursing effect Effects 0.000 abstract 1
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- 238000005516 engineering process Methods 0.000 description 7
- 241000589596 Thermus Species 0.000 description 4
- 238000010564 aerobic fermentation Methods 0.000 description 4
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- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 2
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- 239000002028 Biomass Substances 0.000 description 1
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- 241000868182 Thermus thermophilus HB8 Species 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses a kind of method of extreme thermophilic cooperative fermentation production composite ultra-high temperature compost bacterium, it comprises the following steps：Thermophilic Bacterium Calditerricola satsumensis, Calditerricola yamamurae, Thermus thermophilus are activated seed liquor is made respectively, is inoculated into and is prepared by carrying out fermentation in the sludge fermentation culture medium nursing one's health and optimize；Thermophilic Bacterium wherein is inoculated into fermentation medium fermentation needs to carry out successively according to the order of Calditerricola satsumensis, Calditerricola yamamurae, Thermus thermophilus, and the growth rate of each Thermophilic Bacterium of different phase is controlled by gradient fermentation temperature.This method can realize that cooperative fermentation produces above-mentioned three kinds of Thermophilic Bacterium composite bacteria agents in same fermentation tank, and may advantageously facilitate the stabilization of the synergistic effect and efficient bacterium group's quantity between the middle different Thermophilic Bacteriums that continuously ferment, and improve fermentation efficiency.
Description
Technical field
The invention belongs to field of microbial fermentation, and in particular to a kind of compound Thermophilic Bacteria-Thermus thermophilus
(thermus thermophilus), Calditerricola satsumensis and Calditerricola yamamurae cooperative fermentations produce
The method of combined high temperature compost bacterium.
Background technology
China produces substantial amounts of organic solid castoff, wherein about 4,000,000,000 tons of animal waste, municipal sludge every year
Nearly 35,000,000 tons.These organic solid castoffs produce problem of environmental pollution become increasingly conspicuous, have resulted in great security risk,
Environmental pressure and financial burden.Meanwhile organic waste is also huge resource and energy depot, is contained in China's organic waste
Biomass energy is approximately equivalent to 1,000,000,000 tons of standard coals, while the manurial value rich in nutrients such as N, P, K, organic matters in organic waste
It is suitable with the year production of China's chemical fertilizer.Therefore, how organic waste to be changed into available " money adaptation to local conditions
Source " and " energy ", are neutralizing China's organic solid castoff " environmental pollution " and two big contradiction of agricultural production " scarcity of resources "
It is crucial.
Compost is the important means of organic solid castoff harmless treatment and recycling, the developing history in China
More than one thousand years can be traced, catabolism produces thermal energy under microbial action its essence is organic matter, promotes organic matter to stabilization
Humus conversion process, the harmful organisms such as pathogen in discarded object can be killed, reduce the volume and weight of heap body, be convenient to
Storage and utilization.Compost can not only solve the problem of environmental pollution that organic solid castoff is brought, but also can be by organic solid
Discarded object is converted into organic fertilizer, to keeping and increasing soil fertility, promote agricultural sustainable development to be of great significance.But
Traditional compost technology fermentation temperature is relatively low, fermentation period is long, it is innoxious be not thorough, the shortcomings such as stink is seriously polluted, into
To limit the bottleneck that the versatility is promoted and applied.Microorganism is the driver of aerobic composting process, in degradation of organic substances
Discharging heat at the same time raises heap temperature, and at the same time violent succession occurs for functional microorganism group.In conventional procedure compost
More than 70 DEG C activity that will suppress nearly all microorganism (including thermophilic microorganism) of middle temperature, cause follow-up composting process to be difficult to
Stable operation.2008, Japanese Scientists Y.Oshima had found that extreme thermophilic microorganisms can put forward aerobic compost temperature first
Up to more than 90 DEG C, significantly shorten fermentation period, reduce production cost.On this basis, the researcher such as Zhou Shungui is further
Carry out the research of extreme thermophilic microorganisms separation screening and its function, by the application test in compost engineering for a long time, find
The Thermophilic Bacterium that Thermus and Calditerricola belongs to is to improving compost heap temperature, promoting the effect of compost maturity to show
Write, and based on these extreme thermophilic function stems, develop superhigh temperature aerobic fermentation technology, and obtain and answered in Practical Project
With good result, compost hot stage temperature is brought up to more than 90 DEG C, promote effective killing of pathogenic microorganism, accelerate compost
Humification and maturity, shorten fermentation period, reduce foul smell discharge, improve recycling treatment efficiency.
In superhigh temperature aerobic fermentation technology, Thermus thermophilus, Calditerricola
The Thermophilic Bacterias such as satsumensis and Calditerricola yamamurae are to determine the key factor of fermentation temperature height.So
And these Thermophilic Bacterium growth conditions are harsh, survival rate is low in natural environment, makes it in practical applications easily by other
Indigenous advantage Institute of Micro-biology substitutes and inactivates, so as to influence the treatment effect of superhigh temperature aerobic fermentation technology.Therefore, actually should
These three extreme thermophilic microbial inoculums are periodically added in, to maintain the Microflora of the Thermophilic Bacterium in compost, keep superelevation
Warm aerobic fermentation technology promotes pathogenic microorganism to kill under ultra-high temperature condition, accelerates humification and maturity, shortens fermentation
The technical advantage in cycle.
Since Thermophilic Bacterium growth conditions is harsh, individually fermenting and producing and addition are above-mentioned extreme in practical applications
Thermophilic bacteria agent faces the problems such as bacterium survival rate of high cost, complex for operation step, effective is low.Above-mentioned pole is produced by cooperative fermentation
The composite ultra-high temperature compost bacterium of Thermophilic Bacteria is held, is not only able to make full use of the nutritional ingredient in fermentation medium, improves fermentation
Efficiency, reduces production cost, and is conducive to maintain the steady of Microflora between different Thermophilic Bacteriums in practical applications
It is fixed, simplify operating procedure.However, Thermus thermophilus, Calditerricola satsumensis and
Calditerricola yamamurae are most suitable for growth temperature difference, grow required nutriment difference, utilize tradition to send out
Fermenting process is difficult to realize while fermenting and producing, therefore, currently both at home and abroad to the cooperative fermentation technology of above-mentioned three kinds of Thermophilic Bacteriums
Not yet report.
The content of the invention
It is an object of the invention to disclose a kind of Thermus thermophilus, Calditerricola
Tri- kinds of extreme thermophilic cooperative fermentation production composite ultra-high temperature compost of satsumensis and Calditerricola yamamurae
The method of microbial inoculum.
The technical solution used in the present invention is：
A kind of method of extreme thermophilic cooperative fermentation production composite ultra-high temperature compost bacterium, it comprises the following steps：Will
Thermophilic Bacterium Calditerricola satsumensis, Calditerricola yamamurae, Thermus
Thermophilus is activated seed liquor is made respectively, is inoculated into fermentation medium and is carried out fermentation and be prepared.
Preferably, the method that seed liquor is made in Calditerricola satsumensis is：By 1% inoculum concentration by concentration
For 109Cfu/mL Calditerricola satsumensis are seeded to culture medium, in 75~80 DEG C, 160~180rpm constant temperature
When shaking table culture 16~24 is small；Bacterium solution after culture is inoculated into seeding tank, bacterium solution final concentration of 2% after inoculation, in 75-80
DEG C, throughput 20-100m3Under conditions of/h, 180-220 revs/min of mixing speed, fermentation time 16~24 it is small when.
Preferably, the method that seed liquor is made in Calditerricola yamamurae is：It is by concentration by 1% inoculum concentration
109Cfu/mL Calditerricola yamamurae are seeded to culture medium, in 75~80 DEG C, 160~180rpm constant-temperature tables
Cultivate 16 it is small when；Bacterium solution after culture is inoculated into seeding tank by 2% inoculum concentration, in 75-80 DEG C, throughput 20-
Under conditions of 100m3/h, 180-220 revs/min of mixing speed, fermentation time 16~24 it is small when.
Preferably, the method that seed liquor is made in Thermus thermophilus is：It is by concentration by 1% inoculum concentration
109Cfu/mL Thermus thermophilus activation bacterium solution is seeded in culture medium, and in 70~75 DEG C, 160~180rpm is permanent
When warm shaking table culture 12~24 is small；Bacterium solution after culture is inoculated into seeding tank by 2% inoculum concentration, 70~75 DEG C, ventilation
Measure as 20-100m3Under conditions of/h, 180-220 revs/min of mixing speed, fermentation time 16~24 it is small when.
Preferably, the preparation method of fermentation medium is：It is 3% that municipal sludge, which is adjusted to solid content, adds 0.2%
Bubble enemy, 2g/L dusty yeasts, 0.5g/L ammonium chlorides, 0.5g/L sucrose, 0.5g/L soluble starches, 0.3g/L potassium dihydrogen phosphates, are adjusted
Save pH to 7.0~7.5, add 50g/L complex carriers, stir evenly, 100 DEG C sterilize 45 minutes, be cooled to 80 DEG C it is spare.
Preferably, complex carrier is made of wheat bran and charcoal, is counted in mass ratio, charcoal：Wheat bran=2：1.
Preferably, Thermophilic Bacterium is inoculated into fermentation medium fermentation needs according to Calditerricola
Satsumensis, Calditerricola yamamurae, the order of Thermus thermophilus carry out successively.
Preferably, being inoculated into the method for fermentation medium fermentation is：First by Calditerricola yamamurae seeds
Liquid is seeded in fermentation medium by 1.5% inoculum concentration, in 80 DEG C, throughput 20-100m3/ h, mixing speed 180-220
Rev/min, pre fermentation 6~10 it is small when；Again by 2% inoculum concentration inoculation Calditerricola satsumensis seed liquors, carry out
When primary fermentation 10~16 is small；Finally by 2.5% inoculation Thermus thermophilus seed liquors, fermentation temperature 70~75 is controlled
DEG C, when the 16-24 that ferments after progress is small.
The beneficial effects of the invention are as follows：
(1) due to Calditerricola satsumensis, Calditerricola yamamurae and Thermus
Tri- kinds of Thermophilic Bacteriums of thermophilus are separated from sludge composting, and the nutrient and trace element in sludge can
The growth demand of three kinds of extreme thermophilic microorganisms is substantially met, therefore, the present invention is matched somebody with somebody by the conditioning and optimization of sludge culture medium
Putting realizes cooperative fermentation production Calditerricola satsumensis, Calditerricola in same fermentation tank
Tri- kinds of Thermophilic Bacteriums of yamamurae and Thermus thermophilus.
(2) present invention include pre fermentation, primary fermentation and after ferment three phases, be inoculated with successively in three fermentation stages
Calditerricola yamamurae, Calditerricola satsumensis and Thermus thermophilus seeds
Liquid, and control three kinds of extreme thermophilic microorganisms to avoid fermenting in different phase Fermentative growth speed by gradient fermentation temperature
Mutual suppression in journey between different extreme thermophilic microorganisms, may advantageously facilitate between the middle different Thermophilic Bacteriums that continuously ferment
The stabilization of synergistic effect and efficient bacterium group's quantity, improves fermentation efficiency.
Embodiment
It is prepared by embodiment composite ultra-high temperature compost bacterium
(1) it is 10 by concentration by 1%9The Calditerricola satsumensis YMO81 of cfu/mL and
Calditerricola yamamurae UTM801 activation bacterium solution is seeded to CYS culture mediums respectively, in 80 DEG C, 180rpm constant temperature
When shaking table culture 16 is small.Shaking flask bacterium solution is inoculated into the seeding tank equipped with CYS culture mediums by 2%, 75-80 DEG C, throughput be
20-100m3When/h, 180-220 revs/min of mixing speed, fermentation time 16 are small, Calditerricola is obtained respectively
Satsumensis YMO81 and Calditerricola yamamurae UTM801 seed liquors.
(2) it is 10 by concentration by 1%9Cfu/mL Thermus thermophilus HB8 activation bacterium solution is seeded to DSMZ
In Medium74 culture mediums, in 75 DEG C, when 180rpm constant-temperature tables culture 12 is small.Shaking flask bacterium solution is inoculated into by 2% and is equipped with
In the seeding tank of 74 culture mediums of DSMZ Medium, 70 DEG C, throughput 20-100m3/ h, 180-220 revs/min of mixing speed,
When fermentation time 16 is small.
(3) thickened sludge (sludge fundamental property is shown in Table 1) of dirty Fujian sewage treatment plant is taken, in fermentation tank, is adjusted
Sludge to solid content is 3%, add 0.2% bubble enemy, 2g/L dusty yeasts, 0.5g/L ammonium chlorides, 0.5g/L sucrose, 0.5g/L can
Soluble starch, 0.3g/L potassium dihydrogen phosphates, adjust pH to 7.0, add 50g/L charcoals/wheat bran (2/1) complex carrier, stirring
Uniformly, 100 DEG C sterilize 45 minutes, be cooled to 80 DEG C it is spare.
Table 1. is for examination sludge fundamental property
(4) first Calditerricola yamamurae seed liquors are seeded in fermentation medium by 1.5% inoculum concentration,
In 80 DEG C, throughput 20-100m3When/h, 180-220 revs/min of mixing speed, pre fermentation 6 are small；Again by 2% inoculation
Calditerricola satsumensis seed liquors, when progress primary fermentation 10 is small；Finally by 2.5% inoculation Thermus
Thermophilus (thermus thermophilus) seed liquor, controls 70 DEG C of fermentation temperature, when fermentation 16 is small after progress.
(5) after fermentation, with plate compression method remove supernatant, add 0.5% skimmed milk power/soluble starch/
Glycerine composite protectant, high-speed stirred is uniform, and drying to constant weight, through flour mill grinding, and crosses 80 mesh sieves, packing.
(6) detected by plate count and test tube, in obtained composite ultra-high temperature compost bacterium, total bacteria count is about 7.0
×109Cfu/g, Thermus thermophilus, Calditerricola yamamurae and Calditerricola
The bacterium amount of satsumensis respectively may be about 2.3 × 108cfu/g、4.0×109cfu/g、3.2×109cfu/g。
Influence of the adjusting and configuration of 1 sludge fermentation culture medium of comparative example to composite ultra-high temperature compost bacterium
(1) fermentation medium nurse one's health with configure in, cancel " 2g/L dusty yeasts, 0.5g/L ammonium chlorides, 0.5g/L sucrose,
The use of 0.5g/L soluble starches, 0.3g/L potassium dihydrogen phosphates ", remaining is the same as embodiment 1.
(2) in the composite ultra-high temperature compost bacterium of detection gained, total bacteria count is about 3.0 × 106cfu/g,Thermus
The bacterium amount of thermophilus, Calditerricola yamamurae and Calditerricola satsumensis are respectively about
For 1.3 × 105cfu/g、1.1×106cfu/g、5.2×106Cfu/g, living bacteria count are far below the content of embodiment 1.
Comparative example 2 it is different connect influence of the bacterium order to fermenting and producing composite ultra-high temperature compost bacterium
(1) in fermenting and producing, by Thermus thermophilus are first inoculated with, Calditerricola is inoculated
Yamamurae, is finally inoculated with the order of Calditerricola satsumensis, remaining is the same as embodiment 1.
(2) in the composite ultra-high temperature compost bacterium of detection gained, total bacteria count is about 5.0 × 109cfu/g,Thermus
The bacterium amount of thermophilus, Calditerricola yamamurae and Calditerricola satsumensis are respectively about
For 4.3 × 109cfu/g、1.2×104cfu/g、3.2×104cfu/g.The fast-growth of Thermus thermophilus will press down
The growth of Calditerricola yamamurae and Calditerricola satsumensis processed so that composite ultra-high temperature heap
Effective bacterium number of Calditerricola yamamurae and Calditerricola satsumensis are too low in fertile microbial inoculum.
Claims (8)
1. a kind of method of extreme thermophilic cooperative fermentation production composite ultra-high temperature compost bacterium, it comprises the following steps：By pole
Hold Thermophilic Bacteria Calditerricola satsumensis, Calditerricola yamamurae, Thermus
Thermophilus is activated respectively is made seed liquor, and is inoculated into fermentation medium and carries out fermentation and be prepared.
2. according to the method described in claim 1, it is characterized in that, seed liquor is made in Calditerricola satsumensis
Method be：By 1% inoculum concentration by concentration be 109Cfu/mL Calditerricola satsumensis are seeded to CYS cultures
Base or sludge culture medium, in 75~80 DEG C, when 160~180rpm constant-temperature tables culture 16~24 is small；Bacterium solution after culture is connect
Kind is into seeding tank, bacterium solution final concentration of 2% after inoculation, in 75-80 DEG C, throughput 20-100m3/ h, mixing speed 180-
Under conditions of 220 revs/min, fermentation time 16~24 it is small when.
3. according to the method described in claim 1, it is characterized in that, seed liquor is made in Calditerricola yamamurae
Method is：By 1% inoculum concentration by concentration be 109Cfu/mL Calditerricola yamamurae be seeded to CYS culture mediums or
Sludge culture medium, in 75~80 DEG C, when 160~180rpm constant-temperature tables culture 16 is small；Bacterium solution after culture is pressed to 2% inoculation
Amount is inoculated into seeding tank, in 75-80 DEG C, throughput 20-100m3Under conditions of/h, 180-220 revs/min of mixing speed, send out
When the ferment time 16~24 is small.
4. the according to the method described in claim 1, it is characterized in that, method that seed liquor is made in Thermus thermophilus
It is：By 1% inoculum concentration by concentration be 109Cfu/mL Thermus thermophilus activation bacterium solution is seeded to DSMZ Medium
In 74 culture mediums or sludge culture medium, in 70~75 DEG C, when 160~180rpm constant-temperature tables culture 12~24 is small；After cultivating
Bacterium solution be inoculated into by 2% inoculum concentration in seeding tank, 70~75 DEG C, throughput 20-100m3/ h, mixing speed 180-220
Under conditions of rev/min, fermentation time 16~24 it is small when.
5. according to the method described in claim 1, it is characterized in that, the preparation method of fermentation medium is：By municipal sludge tune
Section to solid content is 3%, add 0.2% bubble enemy, 2g/L dusty yeasts, 0.5g/L ammonium chlorides, 0.5g/L sucrose, 0.5g/L it is solvable
Property starch, 0.3g/L potassium dihydrogen phosphates, adjust pH to 7.0~7.5, add 50g/L complex carriers, stir evenly, 100 DEG C sterilizing
45 minutes, be cooled to 80 DEG C it is spare.
6. according to the method described in claim 5, it is characterized in that, complex carrier is made of wheat bran and charcoal, in mass ratio
Meter, charcoal：Wheat bran=2：1.
7. according to the method described in claim 1, it is characterized in that, Thermophilic Bacterium is inoculated into fermentation medium fermentation needs
According to Calditerricola satsumensis, Calditerricola yamamurae, Thermus thermophilus
Order carry out successively.
8. according to the method described in claim 1, it is characterized in that, being inoculated into the method for fermentation medium fermentation is：First will
Calditerricola yamamurae seed liquors are seeded in fermentation medium by 1.5% inoculum concentration, are in 80 DEG C, throughput
20-100m3When/h, 180-220 revs/min of mixing speed, pre fermentation 6~10 are small；It is inoculated with again by 2% inoculum concentration
Calditerricola satsumensis seed liquors, when progress primary fermentation 10~16 is small；Finally by 2.5% inoculation Thermus
Thermophilus seed liquors, control 70~75 DEG C of fermentation temperature, when the 16-24 that ferments after progress is small.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019061961A1 (en) * | 2017-09-29 | 2019-04-04 | 福建农林大学 | Ultra-high-temperature aerobic fermentation bacteria preparation prepared by using municipal sludge, and preparation method therefor |
| WO2019100579A1 (en) * | 2017-11-27 | 2019-05-31 | 福建农林大学 | Method for rapidly reducing antibiotics and resistance genes in organic solid waste |
| CN114480184A (en) * | 2022-01-13 | 2022-05-13 | 中农创达(北京)环保科技有限公司 | Method for culturing hyperthermophile and preparing freeze-dried fungus powder |
| CN114606163A (en) * | 2022-03-16 | 2022-06-10 | 中南大学 | High-temperature aerobic function strengthening microbial inoculum for organic waste and preparation method and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019061961A1 (en) * | 2017-09-29 | 2019-04-04 | 福建农林大学 | Ultra-high-temperature aerobic fermentation bacteria preparation prepared by using municipal sludge, and preparation method therefor |
| US11898140B2 (en) | 2017-09-29 | 2024-02-13 | Fujian Agriculture And Forestry University | Hyperthermophilic aerobic fermentation inoculant prepared by using municipal sewage sludge and its method |
| WO2019100579A1 (en) * | 2017-11-27 | 2019-05-31 | 福建农林大学 | Method for rapidly reducing antibiotics and resistance genes in organic solid waste |
| CN114480184A (en) * | 2022-01-13 | 2022-05-13 | 中农创达(北京)环保科技有限公司 | Method for culturing hyperthermophile and preparing freeze-dried fungus powder |
| CN114606163A (en) * | 2022-03-16 | 2022-06-10 | 中南大学 | High-temperature aerobic function strengthening microbial inoculum for organic waste and preparation method and application thereof |
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