CN114606163A - High-temperature aerobic function strengthening microbial inoculum for organic waste and preparation method and application thereof - Google Patents
High-temperature aerobic function strengthening microbial inoculum for organic waste and preparation method and application thereof Download PDFInfo
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- CN114606163A CN114606163A CN202210258435.0A CN202210258435A CN114606163A CN 114606163 A CN114606163 A CN 114606163A CN 202210258435 A CN202210258435 A CN 202210258435A CN 114606163 A CN114606163 A CN 114606163A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
Abstract
The invention provides an organic waste high-temperature aerobic function strengthening microbial inoculum and a preparation method and application thereof, which are characterized in that on the basis of microbial enrichments obtained in an extreme environment, an agricultural and forestry organic waste aerobic fermentation high-temperature period and an urban organic waste aerobic fermentation high-temperature period, a Bengal culture medium, a hydrolyzed casein culture medium, a beef extract peptone culture medium and an LB culture medium are used for continuous culture, biological fermentation is carried out, and a compound bacterial liquid is obtained by compounding according to a certain proportion; mixing the compound bacterial liquid with a microbial inoculum carrier, performing filter pressing, and performing vacuum freeze drying to obtain the microbial inoculum; screening high-temperature aerobic function reinforced microbial agents capable of degrading different organic waste components according to the component characteristics of different types of organic waste; different from other organic waste treatment inoculants, the inoculant has the characteristics of high temperature resistance, high degradation rate, obvious reduction effect and the like, can quickly and efficiently utilize organic wastes as resources, and reduces the influence of the organic wastes on the environment.
Description
Technical Field
The invention relates to the field of organic waste recovery, in particular to a high-temperature aerobic function strengthening microbial inoculum for organic waste and a preparation method and application thereof.
Background
The organic solid waste becomes an important source of ecological environment, organic components of municipal solid waste and agricultural non-point source pollution in China, and the problem of efficient utilization of organic waste resources becomes a hotspot and a key point of research. The organic solid waste refers to solid organic articles and substances which are generated in production activities by people and lose utilization value but are discarded or abandoned, and comprises three categories of agricultural organic waste (mainly comprising crop straw vines, livestock and poultry manure, aquatic waste and the like), industrial organic waste (mainly comprising organic waste residues and the like), municipal organic waste (mainly comprising garden greening waste, municipal sludge, slaughter house animal content, kitchen waste and the like). With the rapid development of economy and the continuous improvement of living standard of people in China, the organic solid waste mainly has the following characteristics: the organic solid wastes are various in types and large in production amount; the value is low, the treatment cost is high, and the utilization rate is low; contains a large number of pathogenic bacteria, and can spread diseases; not only brings serious hidden trouble to the urban and rural ecological safety, but also causes the problems of water eutrophication, soil acidification, heavy metal pollution and the like.
The utilization directions of organic solid wastes in China are mainly as follows; the method is characterized by comprising the steps of ecologization, basification, feed conversion, material conversion, fertilizer conversion and energy conversion respectively, wherein the fertilizer conversion and the energy conversion have the highest resource utilization rate. The method for treating the organic solid waste comprises a biological treatment method and a non-biological treatment method. Biological fermentation, biological drying, aerobic fermentation, incineration and landfill are main treatment methods of the kitchen waste. In order to overcome the defects of long treatment period, instability and the like of the traditional biological treatment method, attention is paid to high-temperature aerobic fermentation in the aspect of treating organic solid wastes in recent years, and the high-temperature aerobic fermentation is to finish degradation and conversion of organic matters by utilizing thermophilic aerobic microorganisms under an aerobic condition. No matter anaerobic fermentation, traditional aerobic composting, high-temperature aerobic fermentation and the like can not separate microorganisms, and the microorganisms are main working bodies in the process of reducing the organic solid wastes and play a leading role in the process of treating the organic wastes.
At the present stage, a certain research is carried out on the high-temperature aerobic treatment microbial inoculum for the organic waste, and related microbial inocula can be purchased on the market, but in the practical application of treating the organic waste, the problems of slow degradation rate of the organic waste, poor stress resistance of strains, unobvious reduction effect and the like still exist.
Therefore, the development of the high-temperature aerobic function enhancing microbial inoculum for the organic waste is one of important ways for improving the treatment efficiency of the organic waste, is also an important means for reducing the accumulation amount of the organic waste, and has wide development prospect.
Disclosure of Invention
The invention provides an organic waste high-temperature aerobic function strengthening microbial inoculum and a preparation method and application thereof, and aims to solve the problems in the prior art.
In order to achieve the purpose, the embodiment of the invention provides an organic waste high-temperature aerobic function strengthening microbial inoculum and a preparation method and application thereof.
The invention discloses a preparation method of an organic waste high-temperature aerobic function strengthening microbial inoculum, which is characterized in that microorganisms obtained from different environments are enriched, screened, subjected to primary culture, subjected to secondary culture and compounded to obtain a solid microbial inoculum;
the method specifically comprises the following steps:
s1: acquiring strains from different bacteria sources, carrying out microbial enrichment, then inoculating the strains to a Bengal culture medium, a casein hydrolysate culture medium, a beef extract peptone culture medium and an LB culture medium for continuous culture, carrying out biological fermentation, and compounding according to a certain proportion to obtain a compound bacterial liquid;
s2: drying the modified bentonite and the white carbon black, grinding and sieving by a 100-mesh sieve to obtain microbial inoculum carrier powder;
s3: adding a skim milk powder protective agent into the composite bacterial liquid, fully stirring, adding the microbial inoculum carrier powder, uniformly stirring, and carrying out oscillation adsorption for 6 hours;
s4: and (3) performing solid-liquid separation on the mixture after suction filtration and adsorption for 6 hours to obtain filter residues, and performing vacuum freeze drying, grinding and sieving to obtain the high-temperature aerobic function enhancing microbial inoculum for the organic waste.
Further, the culture medium of the Bengal red comprises: 5g of protein, 10g of glucose, 1g of monopotassium phosphate, 0.5g of magnesium sulfate and 100mL of 1/3000 Bengal solution, wherein the volume is determined to be 1000mL by using deionized water, 0.1g of chloramphenicol and the pH value is about 7.5; the hydrolyzed casein culture medium comprises: 2.68g of soluble starch, 3.57g of hydrolyzed casein, 5.15g of yeast extract and about 7.5 of pH; the beef extract peptone culture medium is as follows: 3.0g of beef extract, 10.0g of peptone and 5.0g of NaCl, and using deionized water to fix the volume to 1000mL, wherein the pH value is 7.5; the LB culture medium is: 10g of tryptone, 5g of yeast extract and 10g of sodium chloride, and the pH value is 7.4.
Further, the bacterial sources are from bottom mud and water samples in Yunnan hot springs, the aerobic fermentation high-temperature period of agricultural and forestry organic wastes and the aerobic fermentation high-temperature period of urban organic wastes. Screening high-temperature aerobic functional reinforced microbial agents capable of degrading different organic waste components according to the component characteristics of different types of organic wastes, such as degradation of lignocellulose in agricultural and forestry organic wastes and degradation of kitchen waste grease, protein and macromolecular organic matters in urban organic wastes.
Further, the Bengal culture medium, the casein hydrolysate culture medium, the beef extract peptone culture medium and the LB culture medium are continuously cultured at 50-55 ℃ to respectively obtain thermophilic bacillus, acinetobacter, coccus and lignocellulose degrading bacteria; the thermophilic bacillus, acinetobacter, coccus and lignocellulose degrading bacteria are as follows 5: 3: 1:1, compounding.
Further, the temperature of the enrichment culture process is 50-55 ℃; the temperature of the continuous culture process is 50-55 ℃, and the temperature is 170-180rpm for 160-180 h.
Further, the microbial inoculum carrier comprises the following components in proportion: white carbon black is 3: 1; and mixing the composite bacterial liquid and the microbial inoculum carrier powder according to a solid-liquid ratio of 1: 1.5-2. The modified bentonite and the white carbon black are mixed according to a certain proportion to be used as a microorganism carrier, the microorganism is protected by a skim milk powder protective agent, the modified bentonite and the white carbon black are uniformly stirred and then placed for a period of time, so that the microorganism carrier fully adsorbs the microorganism, and a vacuum freeze drying method is adopted to obtain the solid microorganism composite microbial inoculum.
Further, the solid-liquid separation is carried out in a plate-and-frame filter pressing mode, and the plate-and-frame filter pressing time is 0.5 h.
Based on a general inventive concept, the invention also provides an organic waste high-temperature aerobic function enhancing microbial inoculum obtained by the preparation method, wherein the microbial inoculum comprises the following microorganisms in percentage by weight: 40-50% of thermophilic bacillus, 15-20% of acinetobacter, 10-15% of coccus and 10-15% of lignocellulose degrading bacteria.
Further, the effective viable count of the microbial inoculum is 8-9.5 multiplied by 109CFU/g。
The invention also provides an application of the ultra-high temperature microbial inoculum for treating the kitchen waste, and the kitchen waste is treated by adopting the organic waste high-temperature aerobic function strengthening microbial inoculum or the organic waste high-temperature aerobic function strengthening microbial inoculum obtained by the preparation method and a high-temperature aerobic fermentation bin.
The scheme of the invention has the following beneficial effects:
(1) the high-temperature aerobic function strengthening microbial inoculum provided by the scheme can be applied to the treatment of agriculture and forestry organic waste and the treatment of urban organic waste, and in use, the existing equipment can be used, so that the content of the organic waste is effectively degraded, the organic waste is reduced efficiently and quickly, and the recycling and harmless treatment of the organic waste are finally realized. The microbial inoculum has the advantages of effectively overcoming the problem of treatment and disposal of the existing organic waste, having great economic significance and environmental protection significance, being convenient to prepare, having low cost, greatly reducing the treatment cost of the organic waste in practical application, efficiently and quickly realizing the reduction of the organic waste and improving the utilization rate of the organic waste. Provides new treatment thought and treatment technology for the reduction, reclamation and harmlessness of organic wastes, has practical economic significance and environmental protection significance, and has wide development prospect
(2) The microbial inoculum is different from other organic waste treatment microbial inocula, has the characteristics of high temperature resistance, high degradation rate, obvious reduction effect and the like, can quickly and efficiently utilize organic wastes as resources, and reduces the influence of the organic wastes on the environment.
(3) The kitchen waste is quickly reduced by utilizing the process of combining the high-temperature aerobic function strengthening microbial inoculum effect of the organic waste and the high-temperature aerobic fermentation bin, the treatment efficiency of the kitchen waste is improved, and the treatment cost of the kitchen waste is reduced.
Detailed Description
In order to make the technical problems, technical solutions and advantages to be solved by the present invention clearer, the following detailed description is given with reference to specific embodiments.
Unless otherwise defined, all terms of art used hereinafter have the same meaning as commonly understood by one of ordinary skill in the art. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the present invention.
Unless otherwise specifically stated, various raw materials, reagents, instruments, equipment and the like used in the present invention may be purchased from commercial sites or may be prepared by existing methods.
The invention provides an organic waste high-temperature aerobic function strengthening microbial inoculum and a preparation method and application thereof, aiming at the existing problems.
In the embodiment of the invention, the microbial strain is from a mud sample and a hot spring water sample in Yunnan hot spring, an aerobic fermentation high-temperature period of agriculture and forestry organic waste and an aerobic fermentation high-temperature period of urban organic waste, and is inoculated into a Bengal culture medium, a casein hydrolysate culture medium, a beef extract peptone culture medium and an LB culture medium for screening, primary culture, secondary culture and compounding to obtain the high-temperature aerobic function reinforced microbial agent with organic matter degradation components.
In the embodiment of the invention, the culture medium of the Bengal red is: 5g of protein, 10g of glucose, 1g of monopotassium phosphate, 0.5g of magnesium sulfate and 100mL of 1/3000 Bengal solution, wherein the volume is determined to be 1000mL by using deionized water, 0.1g of chloramphenicol and the pH value is about 7.5; the hydrolyzed casein culture medium comprises: 2.68g of soluble starch, 3.57g of hydrolyzed casein, 5.15g of yeast extract and about 7.5 of pH; the beef extract peptone culture medium is as follows: 3.0g of beef extract, 10.0g of peptone and 5.0g of NaCl, and using deionized water to fix the volume to 1000mL, wherein the pH value is 7.5; the LB culture medium comprises: 10g of tryptone, 5g of yeast extract and 10g of sodium chloride, and the pH value is about 7.4.
Examples
The high-temperature aerobic function strengthening microbial inoculum for the organic waste is prepared by the following method:
the bottom mud and water sample in the Yunnan hot spring, the aerobic fermentation high-temperature period of the agricultural and forestry organic waste and the aerobic fermentation high-temperature period of the urban organic waste are respectively taken as the sources of the strains. And continuously culturing the Bengal culture medium, the casein hydrolysate culture medium, the beef extract peptone culture medium and the LB culture medium at 50-55 ℃ to respectively obtain microorganisms degrading organic matter components, thermophilic bacillus, acinetobacter, coccus and lignocellulose degrading bacteria according to the ratio of 5: 3: 1:1 to obtain the organic waste high-temperature aerobic function strengthening microbial inoculum.
Drying the modified bentonite and white carbon black, grinding and sieving by a 100-mesh sieve to obtain microbial inoculum carrier powder, adding 3 per mill (w/v) of a protective agent of the skim milk powder into the compound microbial inoculum, fully stirring, adding the microbial inoculum carrier powder, uniformly stirring, and carrying out vibration adsorption for 6 hours. And (3) carrying out plate-and-frame filter pressing to separate solid from liquid, and grinding and sieving the obtained filter residue to obtain the organic waste high-temperature aerobic function strengthening microbial inoculum.
The microbial inoculum comprises the following microbial agents in percentage by weight: 40-50% of thermophilic bacillus; 15-20% of acinetobacter; 10-15% of the genus coccus; lignocellulose degrading bacteria 10-15%. The effective viable count of the microbial inoculum is 8-9.5 multiplied by 109 CFU/g.
Comparative example
Takes sludge and water sample in deep sea environment as microbial inoculumThe source and other steps are the same as the embodiment, and the treatment microbial inoculum is obtained. The effective bacteria number of the microbial inoculum is 3.0-5.2 multiplied by 108CFU/g, the microbial composition is: 30-40% of thermophilic bacillus; 40-60% of the genus coccus.
Application example 1
The method comprises the following steps of collecting agriculture and forestry organic wastes with different sources, classifying the collected agriculture and forestry wastes, carrying out pretreatment such as screening, modulating the water content of the whole system by 55-60% in modes such as squeezing and adding auxiliary materials, and setting two treatments in the application example 1: blank group and add organic waste high temperature aerobic function reinforcement microbial inoculum experimental group, the total weight of the material of whole system is 50 kg. The pretreated organic waste is put into a high-temperature aerobic fermentation chamber high-temperature fermentation chamber (size: 950mm in length, 950mm in width and 1000mm in height), the whole system is stirred uniformly by mechanical stirring, and then the organic waste is treated by the following steps in an experimental group according to the microbial inoculum: material 1: 1000, adding the organic waste high-temperature aerobic function strengthening microbial inoculum, and then uniformly mixing the materials and the microbial inoculum by sufficiently mechanically stirring. In the embodiment 1, the material temperature of the whole fermentation bin is controlled to be 55-65 ℃ by adopting a mode of externally heating the batteries tightly attached to the outer layer of the fermentation bin (externally controlled temperature: 150-200 ℃), the mechanical stirring frequency of the reaction bin is set as positive and negative stirring for 2 minutes, 10 minutes are separated, then positive and negative stirring is carried out for 2 minutes, and the whole reaction system is in an aerobic state by utilizing a suction filtration mode of a fan in the stirring process, which is a continuous fermentation experiment for 38 hours. Sampling is carried out at 0, 6, 12, 24 and 38 hours respectively, and the real-time temperature of the whole system material is detected by a temperature probe in the reaction process by adopting a five-point method. The time interval of sampling in the whole process is set according to the growth rule of the organic waste high-temperature aerobic function strengthening microbial inoculum for sampling at fixed points, and similarly, sampling is carried out by adopting a five-point sampling method every time, and other parameters such as the temperature, the water content (real-time water content detected by a water content detector) and the like of the sampling point are recorded in the sampling process.
Agriculture and forestry organic waste treatment parameter change table
From the above experimental data it can be derived: the water content of the experimental group added with the organic waste high-temperature aerobic function strengthening microbial inoculum is obviously changed compared with that of the blank group, the final water content of the experimental group added with the microbial inoculum is 9 percent, the temperature rise of the experimental group is obviously increased to 60 ℃, and the degradation of organic carbon and lignocellulose is also obviously different compared with that of the blank group. Therefore, the addition of the organic waste high-temperature aerobic function strengthening microbial inoculum is beneficial to the degradation of the components of the agricultural and forestry organic waste and the efficient and rapid reduction of the agricultural and forestry organic waste. Provides a new idea and a new method for the treatment of the organic waste of agriculture and forestry. Is beneficial to the reclamation, harmlessness and reduction of the agriculture and forestry organic waste.
Application example 2
Kitchen waste raw materials are respectively collected from places such as restaurants, dining halls, street snacks and the like, and plastics, paper towels and other impurities in the kitchen waste raw materials are screened out in a screening mode. And the screened kitchen waste passes through a squeezer. The method is characterized by comprising the following steps of performing filter pressing on the waste residues, wherein the filter pressing mainly has the function of crushing the aggregates and other substances in the screened kitchen waste, removing partial water, oil and a small amount of salt in the process, and finally obtaining the kitchen waste tailings with the water content of 55-60 wt% after filter pressing.
Put into high temperature aerobic fermentation storehouse with the kitchen garbage tailings after the preliminary treatment, through mechanical stirring with kitchen garbage tailings and saw wood ash, rice husk or in the wheat bran one or several auxiliary material (moisture content 8% -10%) intensive mixing even to adjust the moisture content of whole reaction system to 50% -60%, this embodiment 2 sets up 2 altogether and handles: blank and add organic waste high temperature aerobic function reinforcing agent experimental group, the total weight of the material of whole system is 50 kg. The whole system is stirred evenly by mechanical stirring, and then the ratio of the microbial inoculum in an experimental group is as follows: material 1: adding an organic waste high-temperature aerobic function reinforcing microbial inoculum according to the proportion of 1000, and then fully and mechanically stirring to uniformly mix the material and the microbial inoculum. In this example 2, the material temperature in the whole fermentation chamber is controlled to be 55-65 ℃ by adopting a mode of externally heating the batteries tightly attached to the outer layer of the fermentation chamber (externally controlled temperature: 150-200 ℃), the mechanical stirring frequency of the reaction chamber is set as positive and negative stirring for 2 minutes, 10 minutes are separated, then positive and negative stirring is carried out for 2 minutes, and the whole reaction system is in an aerobic state by using a suction filtration mode of a fan in the stirring process, which is a continuous fermentation experiment for 38 hours. Sampling is carried out at 0, 6, 12, 24 and 38 hours respectively, and the real-time temperature of the whole system material is detected by a temperature probe in the reaction process by adopting a five-point method. The time interval of sampling in the whole process is set according to the growth rule of the organic waste high-temperature aerobic function strengthening microbial inoculum for fixed-point sampling, and similarly, a five-point sampling method is adopted for sampling each time, and other parameters such as the temperature, the water content (detected by a water content detector) and the like of the sampling point are recorded in the sampling process.
City organic waste-kitchen garbage treatment parameter change table
From the above experimental data it follows that: the water content of the experimental group added with the organic waste high-temperature aerobic function strengthening microbial inoculum is obviously changed compared with that of the blank group, the final water content of the experimental group added with the microbial inoculum is 10 percent, the temperature rise of the experimental group is obviously increased to 59 ℃, and the degradation of organic carbon, protein and fat is also obviously different compared with that of the blank group. Therefore, the addition of the organic waste high-temperature aerobic function strengthening microbial inoculum is beneficial to the degradation of urban organic waste components and the efficient and rapid reduction of urban organic waste. Provides a new idea and a new method for treating the urban organic waste. Is beneficial to the reclamation, harmlessness and reduction of urban organic wastes.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.
Claims (10)
1. The preparation method of the organic waste high-temperature aerobic function strengthening microbial inoculum is characterized by comprising the following steps:
s1: acquiring strains from different bacteria sources, carrying out microbial enrichment, then inoculating the strains to a Bengal culture medium, a casein hydrolysate culture medium, a beef extract peptone culture medium and an LB culture medium for continuous culture, carrying out biological fermentation, and compounding according to a certain proportion to obtain a compound bacterial liquid;
s2: drying the modified bentonite and the white carbon black, grinding and sieving by a 100-mesh sieve to obtain bactericide carrier powder;
s3: adding a skim milk powder protective agent into the composite bacterial liquid, fully stirring, adding the microbial inoculum carrier powder, uniformly stirring, and carrying out oscillation adsorption for 6 hours;
s4: and performing solid-liquid separation on the mixture after suction filtration and adsorption for 6 hours to obtain filter residue, and performing vacuum freeze drying, grinding and sieving to obtain the high-temperature aerobic function enhancing microbial inoculum for the organic waste.
2. The method for preparing the Mongolian red blood, according to claim 1, wherein the culture medium of the Mongolian red blood is: 5g of protein, 10g of glucose, 1g of monopotassium phosphate, 0.5g of magnesium sulfate and 100mL of 1/3000 Bengal solution, wherein the volume is determined to be 1000mL by using deionized water, 0.1g of chloramphenicol and the pH value is about 7.5; the hydrolyzed casein culture medium comprises: 2.68g of soluble starch, 3.57g of hydrolyzed casein, 5.15g of yeast extract and about 7.5 of pH; the beef extract peptone culture medium is as follows: 3.0g of beef extract, 10.0g of peptone and 5.0g of NaCl, and using deionized water to fix the volume to 1000mL, wherein the pH value is 7.5; the LB culture medium comprises: 10g of tryptone, 5g of yeast extract and 10g of sodium chloride, and the pH value is 7.4.
3. The preparation method according to claim 1, wherein the bacterial source is from a substrate sludge and water sample in Yunnan hot spring, an aerobic fermentation high-temperature period of agricultural and forestry organic waste, and an aerobic fermentation high-temperature period of urban organic waste.
4. The method according to claim 1, wherein the Bengal culture medium, the Casein hydrolysate culture medium, the beef extract peptone culture medium and the LB culture medium are continuously cultured at 50-55 ℃ to obtain thermophilic Bacillus, acinetobacter, coccus and lignocellulose-degrading bacteria, respectively; the thermophilic bacillus, acinetobacter, coccus and lignocellulose degrading bacteria are as follows 5: 3: 1:1, compounding.
5. The method of claim 1, wherein the temperature of the enrichment culture process is 50-55 ℃; the temperature of the continuous culture process is 50-55 ℃, and the temperature is 170-180rpm for 160-180 h.
6. The preparation method according to claim 1, wherein the microbial inoculum carrier comprises the following components in proportion: white carbon black is 3: 1; and mixing the composite bacterial liquid and the microbial inoculum carrier powder according to a solid-liquid ratio of 1: 1.5-2.
7. The preparation method according to claim 1, wherein the solid-liquid separation is carried out by adopting a plate-and-frame filter pressing mode, and the plate-and-frame filter pressing time is 0.5 h.
8. A high-temperature aerobic functional strengthening microbial inoculum for organic waste obtained by the preparation method according to any one of claims 1 to 7, which comprises the following microorganisms in percentage by weight: 40-50% of thermophilic bacillus, 15-20% of acinetobacter, 10-15% of coccus and 10-15% of lignocellulose degrading bacteria.
9. The organic waste high-temperature aerobic functional strengthening bacterial agent as claimed in claim 8, wherein the effective viable count of the bacterial agent is 8-9.5 x 109CFU/g。
10. The application of the ultra-high temperature microbial inoculum for treating the kitchen waste is characterized in that the kitchen waste is treated by adopting the method of combining the organic waste high temperature aerobic function strengthening microbial inoculum according to the claim 8 or the organic waste high temperature aerobic function strengthening microbial inoculum obtained by the preparation method according to any one of the claims 1 to 7 with a high temperature aerobic fermentation bin.
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