CN108048099A - Salt-soda soil auxotype modifying agent - Google Patents

Salt-soda soil auxotype modifying agent Download PDF

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Publication number
CN108048099A
CN108048099A CN201711184780.XA CN201711184780A CN108048099A CN 108048099 A CN108048099 A CN 108048099A CN 201711184780 A CN201711184780 A CN 201711184780A CN 108048099 A CN108048099 A CN 108048099A
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China
Prior art keywords
modifying agent
salt
auxotype
soda soil
coal gangue
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Withdrawn
Application number
CN201711184780.XA
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Chinese (zh)
Inventor
王胜
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Shandong Scenic Tourism Development Co Ltd
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Shandong Scenic Tourism Development Co Ltd
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Priority to CN201711184780.XA priority Critical patent/CN108048099A/en
Publication of CN108048099A publication Critical patent/CN108048099A/en
Withdrawn legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01BSOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
    • A01B79/00Methods for working soil
    • A01B79/02Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00Soil-conditioning materials or soil-stabilising materials
    • C09K17/40Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • CCHEMISTRY; METALLURGY
    • C09DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09KMATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00Agricultural use

Abstract

The present invention relates to modifying agent technical field more particularly to a kind of salt-soda soil auxotype modifying agents.It is prepared including amino acid solution;It is prepared by organic additive;It is prepared by high sulfur coal gangue composite bacterium powder;Whereby, the present invention reacts and then filters to obtain amino acid solution by the way that poultry feather to be made suspension and be hydrolyzed again.By the way that the expansion of a variety of strains is connected on culture medium, culture is ripe in the incubator, obtains mixed bacteria liquid;The high sulfur coal gangue powder for pulverizing and sieving and being ultrasonically treated and mixed bacteria liquid mixed fermentation are obtained into high sulfur coal gangue composite bacteria liquid again;Then composite bacteria liquid is inoculated into solid fermentation matrix, fermentating drying crushes, and obtains high sulfur coal gangue composite bacterium powder.Afterwards again by amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by being mixed in a certain ratio and stir evenly to obtain salt-soda soil auxotype modifying agent;Greatly improve soil regime and plant growth index after modifying agent application, improve crop yield.

Description

Salt-soda soil auxotype modifying agent
Technical field
The present invention relates to modifying agent technical field more particularly to a kind of salt-soda soil auxotype modifying agents.
Background technology
China's saline-alkali soil area is very big, and type is complicated, and all kinds of saline-alkali soil gross areas in the whole nation are up to 1,487,000,000 mu.Due to soil The accumulation of interior a large amount of salt causes a series of deterioration of soil physical properties:Structure is viscous, and aeration is poor, and unit weight is high, in soil temperature It rises slowly, aerobic microorganism activity is poor in soil, and difference release is slow, and infiltration coefficient is low, and capillarity is strong, more causes topsoil The saliferous aggravation of earth.
With continually developing for Coastal beach, many difficulties are encountered during the improvement and utilization to salt-soda soil.In order to Coastal tidal soil is made full use of, people take trench digging precipitation salt discharge, and fresh water perfusion pressure salt sets drenching layer antiseepage more every salt etc. Kind method carries out salt discharge drop alkali improvement, to reach Soil Desalting and prevent " accumulation of salt in the surface soil " purpose.But since salt-soda soil soil is hardened, poor Lean, fertility is low and soil pH value is higher, is unfavorable for plant growth.
Soil conditioner plays an important role in alkaline land improving, and microbial bacterial agent is also a developing direction, but There are following defects for existing soil conditioner:(1) microorganism in modifying agent transport, easily inactivate in storage process without It can play a role;(2) it is limited to improve salt-soda soil ability, it is impossible to while reduce soil pH and basicity;(3) to soil with organic matter The raising scarce capacity of content;(4) it is bad to the improvement of soil water retention hole;(5) Soil Microorganism after improveing Negligible amounts.
In summary, the existing technology has inconveniences and defects in actual use, so it is necessary to be improved.
The content of the invention
For it is above-mentioned the defects of, it is an object of the invention to provide a kind of salt-soda soil auxotype modifying agent, by by birds Feather is made suspension and reaction is hydrolyzed again and then filters to obtain amino acid solution.By the way that the expansion of a variety of strains is connected to culture medium On, culture is ripe in the incubator, obtains mixed bacteria liquid;The high sulfur coal gangue powder and Mixed Microbes that will be pulverized and sieved and be ultrasonically treated again Liquid mixed fermentation obtains high sulfur coal gangue composite bacteria liquid;Then composite bacteria liquid is inoculated into solid fermentation matrix, fermentating drying powder It is broken, obtain high sulfur coal gangue composite bacterium powder.Afterwards again by amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by pressing Certain proportion mixes and stirs evenly and to obtain salt-soda soil auxotype modifying agent;Greatly improve soil regime and work after modifying agent application Object growth indexes improve crop yield.
To achieve these goals, the present invention provides a kind of salt-soda soil auxotype modifying agent, including:
A:It is prepared by amino acid solution
A. poultry feather is prepared into feather suspension;
B. suspension is placed in reaction kettle, sequentially adds urea, pancreatin, sodium hydrogensulfite, 170-190 turns/min stirrings When 2-2.5 is small;Be warming up to 50 DEG C, add in neutral proteinase, 170-190 turns/min stirrings 12-14 it is small when after adjust pH value 6.8- 7.2;When hydrolysis 20-24 is small under 1.1-1.3 atmospheric pressure afterwards, then adds in manganese chloride solution and adjust pH value 5.0-5.2, When 80-90 DEG C of heat preservation 5-7 is small;The manganese chloride solution concentration is 10-12mol/L;
C. liquid b step obtained obtains amino acid solution by 500-600 mesh glass fiber filters;
B:It is prepared by organic additive
Urea, double superhosphate phosphate fertilizer and KCl are pressed 1:1:1 ratio mixes and water is added to adjust water content to 35- 40%;
C:It is prepared by high sulfur coal gangue composite bacterium powder
A. strain expansion is connected on fresh test tube beef extract-peptone slant medium;It trains within 4 days in 30 DEG C of incubators It forms ripe, obtains strain fermentating liquid;
Thiobacillus thioxidans, bacillus subtilis, gel-shaped bacillus, thin yellow chain are prepared using a steps respectively Mould, black-koji mould, the bacterium solution of Trichoderma asperellum and bacillus licheniformis and by them according to volume ratio 4:1:1:1:1:1:1 Ratio mix to obtain mixed bacteria liquid;
B., high sulfur coal gangue is ground into the fritter of 45-55mm, 200 mesh sieves are crossed after grinding, then 50HZ ultrasonications 35 minutes, obtain high sulfur coal gangue powder;
C. the mixed bacteria liquid of weight ratio 5-7% is added in the high sulfur coal gangue powder, then plus water modulation is aqueous Measure 23-27%;At 30-35 DEG C and it is every 18 it is small when stirring once under conditions of ferment 20-23 days, obtain high sulfur coal gangue compound bacteria Liquid;
D. as the bacterium number in composite bacteria liquid described in step c >=2,000,000,000/ml, composite bacteria liquid is inoculated into solid fermentation matrix In, it ferments 5 days and low temperature drying crushes to obtain high sulfur coal gangue composite bacterium powder;Inoculum concentration is volume ratio 5-7%;
D:By the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 45~60:15 ~40:15~25 mix and stir evenly to obtain salt-soda soil auxotype modifying agent.
The step A poultry feather is chicken feather, drake feather, goose feather.
Strain described in the step C uses test tube slant preserving process;It is preserved in 0-4 DEG C of refrigerator.
Solid fermentation matrix is described in the step C, and vinasse, vinegar grain, wheat bran mass ratio are 3~5:4~6:1~3, 120 DEG C of sterilizing 30min.
The D steps, by the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 55:35:15 mix and stir evenly to obtain salt-soda soil auxotype modifying agent.
The D steps, by the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 50:30:20 mix and stir evenly to obtain salt-soda soil auxotype modifying agent.
The D steps, by the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 60:15:25 mix and stir evenly to obtain salt-soda soil auxotype modifying agent.
The D steps, by the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 45:40:15 mix and stir evenly to obtain salt-soda soil auxotype modifying agent.
The method of administration of the modifying agent is:By prepartion of land, turn over, harrow mill after apply fertilizer 500-700KG/ mus, then into 1-2 intersection rotary tillage of row, 12-15 centimetres of rotary tillage depth are irrigated after then adding in the modifying agent rotary tillage.
The modifying agent carries out the application experiment of salt-soda soil corn planting, is using result:Emergence rate increases above 10%, strain Height increases above 5%, and stem slightly increases above 8%, and root activity increases above 30%, and biomass increases above 0.8g/ plants.
It is an object of the invention to provide a kind of salt-soda soil auxotype modifying agent, by the way that poultry feather is made suspension again Reaction is hydrolyzed and then filters to obtain amino acid solution.By the way that the expansion of a variety of strains is connected on culture medium, cultivate in the incubator Maturation obtains mixed bacteria liquid;The high sulfur coal gangue powder for pulverizing and sieving and being ultrasonically treated and mixed bacteria liquid mixed fermentation are obtained into high-sulfur again Gangue composite bacteria liquid;Then composite bacteria liquid is inoculated into solid fermentation matrix, fermentating drying crushes, and obtains high sulfur coal gangue and answers Close bacterium powder.Afterwards again by amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by being mixed in a certain ratio and stir It is even to obtain salt-soda soil auxotype modifying agent;Greatly improve soil regime and plant growth index after modifying agent application, improve Crop yield.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to Limit the present invention.
The present invention provides a kind of salt-soda soil auxotype modifying agents, comprise the following steps:
A:It is prepared by amino acid solution
A. poultry feather is prepared into feather suspension;
B. suspension is placed in reaction kettle, sequentially adds urea, pancreatin, sodium hydrogensulfite, 170-190 turns/min stirrings When 2-2.5 is small;Be warming up to 50 DEG C, add in neutral proteinase, 170-190 turns/min stirrings 12-14 it is small when after adjust pH value 6.8- 7.2;When hydrolysis 20-24 is small under 1.1-1.3 atmospheric pressure afterwards, then adds in manganese chloride solution and adjust pH value 5.0-5.2, When 80-90 DEG C of heat preservation 5-7 is small;The manganese chloride solution concentration is 10-12mol/L;
C. liquid b step obtained obtains amino acid solution by 500-600 mesh glass fiber filters;
B:It is prepared by organic additive
Urea, double superhosphate phosphate fertilizer and KCl are pressed 1:1:1 ratio mixes and water is added to adjust water content to 35- 40%;
C:It is prepared by high sulfur coal gangue composite bacterium powder
A. strain expansion is connected on fresh test tube beef extract-peptone slant medium;It trains within 4 days in 30 DEG C of incubators It forms ripe, obtains strain fermentating liquid;
Thiobacillus thioxidans, bacillus subtilis, gel-shaped bacillus, thin yellow chain are prepared using a steps respectively Mould, black-koji mould, the bacterium solution of Trichoderma asperellum and bacillus licheniformis and by them according to volume ratio 4:1:1:1:1:1:1 Ratio mix to obtain mixed bacteria liquid;
B., high sulfur coal gangue is ground into the fritter of 45-55mm, 200 mesh sieves are crossed after grinding, then 50HZ ultrasonications 35 minutes, obtain high sulfur coal gangue powder;
C. the mixed bacteria liquid of weight ratio 5-7% is added in the high sulfur coal gangue powder, then plus water modulation is aqueous Measure 23-27%;At 30-35 DEG C and it is every 18 it is small when stirring once under conditions of ferment 20-23 days, obtain high sulfur coal gangue compound bacteria Liquid;
D. as the bacterium number in composite bacteria liquid described in step c >=2,000,000,000/ml, composite bacteria liquid is inoculated into solid fermentation matrix In, it ferments 5 days and low temperature drying crushes to obtain high sulfur coal gangue composite bacterium powder;Inoculum concentration is volume ratio 5-7%;
D:By the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 45~60:15 ~40:15~25 mix and stir evenly to obtain salt-soda soil auxotype modifying agent;
Preferably, step A poultry feather of the invention is chicken feather, drake feather, goose feather;
Preferably, the amino acid content of amino acid solution described in step A of the invention is 220-250g/L.
Preferably, strain described in step C of the invention uses test tube slant preserving process;It is preserved in 0-4 DEG C of refrigerator.
Preferably, solid fermentation matrix is described in step C of the invention, vinasse, vinegar grain, wheat bran mass ratio for 3~ 5:4~6:1~3,120 DEG C of sterilizing 30min;
The present invention method of administration be:By prepartion of land, turn over, harrow mill after apply fertilizer 500-700KG/ mus, then carry out 1-2 Secondary intersection rotary tillage, 12-15 centimetres of rotary tillage depth are irrigated after then adding in the made modifying agent rotary tillage of the present invention.
To obtain the preferred forms of production method, inventor has carried out salt-soda soil corn using different embodiments and has applied With experiment, method of administration used is same as above, and the nutrients chemical fertilizer of simple substance such as use the results are shown in Table 1 as comparative example;Following embodiment is only The part different from above-mentioned steps is listed, identical content is no longer listed.
Embodiment one:
The D steps, by the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 55:35:15 mix and stir evenly to obtain salt-soda soil auxotype modifying agent;
By above-mentioned salt-soda soil auxotype modifying agent for salt-soda soil application experiment, application effect is:Emergence rate increases by 17%, Plant height increases by 8%, and stem slightly increases by 12%, and root activity increases by 33.1%, and biomass increases 1.31g/ plants.
Embodiment two:
The D steps, by the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 50:30:20 mix and stir evenly to obtain salt-soda soil auxotype modifying agent;
By above-mentioned salt-soda soil auxotype modifying agent for salt-soda soil application experiment, application effect is:Emergence rate increases by 19%, Plant height increases by 9%, and stem slightly increases by 13%, and root activity increases by 34.3%, and biomass increases 0.89g/ plants.
Embodiment three:
The D steps, by the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 60:15:25 mix and stir evenly to obtain salt-soda soil auxotype modifying agent;
By above-mentioned salt-soda soil auxotype modifying agent for salt-soda soil application experiment, application effect is:Emergence rate increases by 15%, Plant height increases by 8.5%, and stem slightly increases by 11%, and root activity increases by 31.9%, and biomass increases 1.01g/ plants.
Example IV:
The D steps, by the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 45:40:15 mix and stir evenly to obtain salt-soda soil auxotype modifying agent;
By above-mentioned salt-soda soil auxotype modifying agent for salt-soda soil application experiment, application effect is:Emergence rate increases by 18%, Plant height increases by 9.5%, and stem slightly increases by 11%, and root activity increases by 33.6%, and biomass increases 0.94g/ plants.
The implementation procedure of other embodiments repeats no more, and table 1 is the various embodiments described above and other section Example is not shown Data.It understands that the present invention carries out the application experiment of salt-soda soil corn planting by above example, is using result:Emergence rate increases Add more than 10%, plant height increases above 5%, and stem slightly increases above 8%, and root activity increases above 30%, and biomass increases super Cross 0.8g/ plants.
Table 1
Note:Application effect is got by radix calculating of comparative example.
In conclusion it is an object of the invention to provide a kind of salt-soda soil auxotype modifying agent, by by poultry feather system Reaction is hydrolyzed again into suspension and then filters to obtain amino acid solution.By the way that the expansion of a variety of strains is connected on culture medium, training It supports and maturation is cultivated in case, obtain mixed bacteria liquid;The high sulfur coal gangue powder for pulverizing and sieving and being ultrasonically treated is mixed with mixed bacteria liquid again Ferment to obtain high sulfur coal gangue composite bacteria liquid;Then composite bacteria liquid is inoculated into solid fermentation matrix, fermentating drying crushes, and obtains high Sulphur gangue composite bacterium powder.Amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder are pressed by certain ratio again afterwards Example mixes and stirs evenly to obtain salt-soda soil auxotype modifying agent;Greatly improve soil regime and plant growth after modifying agent application Index improves crop yield.
Certainly, the present invention can also have other various embodiments, without deviating from the spirit and substance of the present invention, ripe It knows those skilled in the art and makes various corresponding changes and deformation, but these corresponding changes and change in accordance with the present invention Shape should all belong to the protection domain of appended claims of the invention.

Claims (10)

1. a kind of salt-soda soil auxotype modifying agent, which is characterized in that including:
A:It is prepared by amino acid solution
A. poultry feather is prepared into feather suspension;
B. suspension is placed in reaction kettle, sequentially adds urea, pancreatin, sodium hydrogensulfite, 170-190 turns/min stirrings 2- 2.5 it is small when;Be warming up to 50 DEG C, add in neutral proteinase, 170-190 turns/min stirrings 12-14 it is small when after adjust pH value 6.8- 7.2;When hydrolysis 20-24 is small under 1.1-1.3 atmospheric pressure afterwards, then adds in manganese chloride solution and adjust pH value 5.0-5.2, When 80-90 DEG C of heat preservation 5-7 is small;The manganese chloride solution concentration is 10-12mol/L;
C. liquid b step obtained obtains amino acid solution by 500-600 mesh glass fiber filters;
B:It is prepared by organic additive
Urea, double superhosphate phosphate fertilizer and KCl are pressed 1:1:1 ratio mixes and water is added to adjust water content to 35-40%;
C:It is prepared by high sulfur coal gangue composite bacterium powder
A. strain expansion is connected on fresh test tube beef extract-peptone slant medium;It is trained within 4 days in 30 DEG C of incubators It is ripe, obtain strain fermentating liquid;
Using a steps prepare respectively Thiobacillus thioxidans, bacillus subtilis, gel-shaped bacillus, streptomyces microflavus, Black-koji mould, Trichoderma asperellum and the bacterium solution of bacillus licheniformis and by them according to volume ratio 4:1:1:1:1:1:1 ratio Example mixes to obtain mixed bacteria liquid;
B., high sulfur coal gangue is ground into the fritter of 45-55mm, 200 mesh sieves are crossed after grinding, then 50HZ ultrasonications 35 are divided Clock obtains high sulfur coal gangue powder;
C. the mixed bacteria liquid of weight ratio 5-7% is added in the high sulfur coal gangue powder, then plus water modulates water content 23- 27%;At 30-35 DEG C and it is every 18 it is small when stirring once under conditions of ferment 20-23 days, obtain high sulfur coal gangue composite bacteria liquid;
D. as the bacterium number in composite bacteria liquid described in step c >=2,000,000,000/ml, composite bacteria liquid is inoculated into solid fermentation matrix, It ferments 5 days and low temperature drying crushes to obtain high sulfur coal gangue composite bacterium powder;Inoculum concentration is volume ratio 5-7%;
D:By the amino acid solution, organic additive and high sulfur coal gangue composite bacterium powder by weight 45~60:15~40: 15~25 mix and stir evenly to obtain salt-soda soil auxotype modifying agent.
2. auxotype modifying agent in salt-soda soil according to claim 1, which is characterized in that the step A poultry feather is chicken Hair, drake feather, goose feather.
3. auxotype modifying agent in salt-soda soil according to claim 1, which is characterized in that strain is equal described in the step C Using test tube slant preserving process;It is preserved in 0-4 DEG C of refrigerator.
4. auxotype modifying agent in salt-soda soil according to claim 1, which is characterized in that solid described in the step C is sent out Ferment matrix is that vinasse, vinegar grain, wheat bran mass ratio are 3~5:4~6:1~3,120 DEG C of sterilizing 30min.
5. auxotype modifying agent in salt-soda soil according to claim 1, which is characterized in that the D steps, by the amino acid Solution, organic additive and high sulfur coal gangue composite bacterium powder are by weight 55:35:15 mix and stir evenly to obtain salt-soda soil auxotype Modifying agent.
6. auxotype modifying agent in salt-soda soil according to claim 1, which is characterized in that the D steps, by the amino acid Solution, organic additive and high sulfur coal gangue composite bacterium powder are by weight 50:30:20 mix and stir evenly to obtain salt-soda soil auxotype Modifying agent.
7. auxotype modifying agent in salt-soda soil according to claim 1, which is characterized in that the D steps, by the amino acid Solution, organic additive and high sulfur coal gangue composite bacterium powder are by weight 60:15:25 mix and stir evenly to obtain salt-soda soil auxotype Modifying agent.
8. auxotype modifying agent in salt-soda soil according to claim 8, which is characterized in that the D steps, by the amino acid Solution, organic additive and high sulfur coal gangue composite bacterium powder are by weight 45:40:15 mix and stir evenly to obtain salt-soda soil auxotype Modifying agent.
9. the salt-soda soil auxotype modifying agent according to claim 1~8 any one, which is characterized in that the modifying agent Method of administration be:By prepartion of land, turn over, harrow mill after apply fertilizer 500-700KG/ mu, then carry out 1-2 time intersect rotary tillage, revolve 12-15 centimetres of tilling depth degree is irrigated after then adding in the modifying agent rotary tillage.
10. auxotype modifying agent in salt-soda soil according to claim 9, which is characterized in that the modifying agent carries out salt-soda soil Corn planting application is tested, and is using result:Emergence rate increases above 10%, and plant height increases above 5%, and stem slightly increases above 8%, root activity increases above 30%, and biomass increases above 0.8g/ plants.
CN201711184780.XA 2017-11-23 2017-11-23 Salt-soda soil auxotype modifying agent Withdrawn CN108048099A (en)

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Application Number Priority Date Filing Date Title
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109089454A (en) * 2018-09-21 2018-12-28 佛山市森昂生物科技有限公司 A kind of desertification land control method
CN110218679A (en) * 2019-06-21 2019-09-10 漳州三炬生物技术有限公司 A kind of secondary salinization soil remediation microbial inoculum and its application

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109089454A (en) * 2018-09-21 2018-12-28 佛山市森昂生物科技有限公司 A kind of desertification land control method
CN110218679A (en) * 2019-06-21 2019-09-10 漳州三炬生物技术有限公司 A kind of secondary salinization soil remediation microbial inoculum and its application

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Application publication date: 20180518