CN103320137A - Composite microbiological bacteriocin saline-alkali soil improver, and preparation method and applications thereof - Google Patents
Composite microbiological bacteriocin saline-alkali soil improver, and preparation method and applications thereof Download PDFInfo
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Abstract
The invention relates to a composite microbiological bacteriocin saline-alkali soil improver, and a preparation method and applications thereof. The composite microbiological bacteriocin saline-alkali soil improver comprises a microbial agent, an organic liquid and a matrix. The microbial agent specifically comprises bacillus subtilis and bacillus megaterium, and nutrient substances of protease, lipase, alfa-amylase, amino acid and the like can be genereated by fermentation of the microbial bacteriocins; and the microbial agent can be used to adjust soil acidity-alkalinity, reduce saline-alkali toxicity, adjust rice root system microbial environment, accelerate transition of trace elements comprising nitrogen, phosphorus and potassium, and accelerate root growth, root increase, seedling development and seedling strengthening, and helps to lay a good foundation for increase of rice production.
Description
Technical field
The present invention relates to bio-bacterial manure technology and Saline-alkali Field Control field, particularly a kind of complex microorganism rhzomorph alkaline land modifying agent and preparation method thereof and application.
Background technology
Ecology of alkali saline land reparation and improvement are engineerings of benefiting the nation and the people, and both can increase cultivated area, excavate agriculture potentiality, Guarantee Grain Production, more be conducive to the development of agricultural industry.Be conducive to enlarge foodstuff planting area, ensure the effective supply of grain, in Chinese Inner Mongol, good achievement has been obtained in the areas such as Jilin aspect Saline-alkali Field Control, patent also has much aspect Saline-alkali Field Control, for example: the Chinese patent CN101481281A (applying date: on February 3rd, 2009, application number: 200910066498.0, applicant: Xinshiji Agriculture Science-Technology Co., Ltd., Jilin), the Chinese patent CN 100482064C (applying date: on September 14th, 2007, application number: 20071012790.9, applicant: utilize the calcium ion in the coal desulfurize agent gypsum will be the cation replacements such as sodium ion in the soil colloid out Tsing-Hua University), form calcium colloid and organic and inorganic complex body, change soil aggregate, reduced the basicity of soil, improved soil permeability.The effect of Sodium Bicarbonate Soil Management agent is that integrated chemical salify, complexing, neutralization, ion-exchange, desorption are attached, passivation and fixing and physical chemistry functions such as the anti-accumulation of salt in the surface soil and breakdown of emulsion are integrated.Aspect alkaline land improving, all obtained regulation effect.But, to the reparation of soil microenvironment, remove the yin, yang ion harmful to rice root, solubility Na
+Ion does not well solve.
The main chloride in saltings or the higher salinization soil of vitriol, soil are alkalescence, but the pH value is not necessarily very high.Alkaline earth refers to carbonate containing or heavy phosphatic soil, and pH value is higher, and soil is alkalescence, and the organic content of saline-alkali soil is few, and soil fertility is low, and physicochemical character is poor, and the yin, yang ion that crop is harmful to is many, has a strong impact on the crop short seedling of taking root, and makes grain yield low.
Summary of the invention
The objective of the invention is the defective according to above-described alkaline land modifying agent, provide a kind of complex microorganism rhzomorph saltings to keep a full stand of seedings, utilize microorganism to the repairing effect of soil, the improvement edatope, decompose objectionable impurities and corrupt substance in the soil, regulate the molecular structure of soil, eliminate soil half, chessom, keep the soil micro-ecosystem balance, make fertile soil.
Above-mentioned purpose of the present invention will further describe and embody by following detailed description:
A kind of complex microorganism rhzomorph alkaline land modifying agent, described modifying agent comprises microorganism, organic liquor and matrix.
Further, described microorganism comprises subtilis (Bacillus subtilis) and bacillus megaterium (Bacillus megaterium), and the ratio of weight and number of described subtilis and bacillus megaterium is:
Subtilis 60～80
Bacillus megaterium 20～40;
Optimum ratio of weight and number is:
Subtilis 70
Bacillus megaterium 30.
Further, described subtilis is that described subtilis is the mixture of subtilis CGMCC7296, subtilis CGMCC 7297, subtilis CGMCC 7298; Described bacillus megaterium is CGMCC 7337.
Further, comprise the proteolytic enzyme that described microbiobacterial agent fermentation produces in the described organic liquor, lipase, α-amylase, amino acid.
Further, described matrix comprises chicken manure or cow dung, brown coal, ground phosphate rock, medical stone, and the ratio of weight and number of described chicken manure or cow dung, brown coal, ground phosphate rock, medical stone is:
Further, described chicken manure or cow dung are through 200～400 ℃ of high-temperature sterilizations; Described brown coal contain 20% humic acid; Described ground phosphate rock, medical stone are all more than 300 orders.
Another object of the present invention realizes by following scheme:
A kind of preparation method of complex microorganism rhzomorph alkaline land modifying agent, the manufacturing process steps of described modifying agent is as follows:
Described complex microorganism rhzomorph alkaline land modifying agent is obtained by 3 bacillus subtilis (Bacillus subtilis) and 1 strain bacillus megaterium (Bacillus megaterium) fermentation;
Described 3 bacillus subtilis are subtilis CGMCC 7296, subtilis CGMCC 7297, subtilis CGMCC 7298; Described bacillus megaterium is CGMCC 7337.
1) bacterial classification is prepared
With described 3 strain millet straw genus bacillus and the respectively separately activation on the ordinary broth inclined-plane of 1 strain bacillus megaterium, the bacterial classification after obtaining activating; Bacterial classification after the described activation is transferred to respectively in the flat bottle, cultivates 24h, make respectively No. 1 seed suspension, No. 2 seed suspension, No. 3 seed suspension, No. 4 seed suspension with 0.1 peptone aseptic aqueous solution for 28～30 ℃;
2) cultivation of seeding tank
Described 1～No. 3 seed suspension all is linked into the A seeding tank, and described No. 4 seed suspension are linked into the B seeding tank;
The prescription of substratum and ratio of weight and number thereof are in the A seeding tank:
The temperature of described A seeding tank is 28～34 ℃, and the pH of cultivation is 7.5～8.5;
The prescription of substratum and ratio of weight and number thereof are in the B seeding tank:
With described A, B seeding tank when the slack tank, respectively sterilization 45 minutes under 125 ℃/0.15mpa pressure, sterilization 30 minutes under 120 ℃/0.11mpa is cooled to 35 ℃ after the charging, 500 liters seeding tank constant volume 50%, described A, B seeding tank inoculate respectively 6～9 * 10
8Cfu; After the inoculation at 30～35 ℃, air flow 1: 1 (v: v), rotating speed 190～220r/min, after 7～9 hours, thalline reaches 1.0～1.5 * 10 in the bacterium liquid under the condition of tank pressure 0.05～0.08mpa
9/ ml, can culture transferring to the fermentor tank fermentation culture;
3) ferment tank
The culture medium prescription of fermentor tank and ratio of weight and number thereof are:
Described fermentor tank is under slack tank, and sterilization is 45 minutes under 125 ℃/0.15mpa pressure, sterilizes 30 minutes under 120 ℃/0.11mpa after the charging, is cooled to 35 ℃, 5000 liters fermentor tank constant volume 60%; Bacterium liquid after described A, the B seed tank culture is seeded in the described fermentor tank, and the leavening temperature of described fermentor tank is 28～34 ℃, and the pH of fermentation culture is 7.5～8.5; The air flow in 4 hours of fermenting is that (v: v), air flow increased to 1: 1 (v: v), fermentation total time is 36～38 hours, and thalline reaches 0.8～1.0 * 10 in the bacterium liquid after 4 hours in 1: 0.5
10/ ml, spore forming rate reaches 80%, the bacterium liquid after namely obtaining fermenting;
4) be adsorbed in matrix
Bacterium liquid and matrix after the described fermentation are stirred, according to 20～30L/m
3Be adsorbed in matrix, namely obtain described complex microorganism rhzomorph alkaline land modifying agent.
Further, described matrix comprises chicken manure or cow dung, brown coal, ground phosphate rock, medical stone.
Further, described modifying agent requires to be not less than 5 * 10 through fungal counting
9/ g.
Further, when described modifying agent can be applicable to the rice transplanting of paddy rice saltings, band was fertile, the band medicine, carry disease germs and base fertilizer, dressing of turnning green stage use the same period, and consumption is 25kg/ mu.
The present invention's beneficial effect compared to existing technology is:
1, complex microorganism rhzomorph alkaline land modifying agent of the present invention, has the breeding growth around rice root of Salt And Alkali Tolerance microorganism, microorganism can constantly discharge protein, multivitamin, organic acid, amino acid, proteolytic enzyme, saccharifying enzyme, lipase, amylase etc. in process of growth, can eliminate fast the yin, yang ion harmful to rice root;
2, the beneficial microorganism among the present invention is in the breeding metabolic process, sustainable secretion growth hormone isoreactivity material, can promote that paddy rice takes root, root is white, root capillary radical amount doubles above, fertilizer sucting ability strengthens, stem is thick, seedling is strong, makes well developed root system, rice seedlings stalwartness, for increasing production of rice is laid a good foundation.
Embodiment
Further specify the present invention below in conjunction with embodiment, but protection scope of the present invention is not limited to embodiment.
Embodiment 1
A kind of complex microorganism rhzomorph alkaline land modifying agent, described modifying agent comprises microorganism, organic liquor and matrix.
Further, described microorganism comprises subtilis (Bacillus subtilis) and bacillus megaterium (Bacillus megaterium), and the ratio of weight and number of described subtilis and bacillus megaterium is:
Subtilis 60～80
Bacillus megaterium 20～40;
Optimum ratio of weight and number is:
Subtilis 70
Bacillus megaterium 30.
Further, described subtilis is that described subtilis is the mixture of subtilis CGMCC7296, subtilis CGMCC 7297, subtilis CGMCC 7298; Described bacillus megaterium is CGMCC 7337.
Comprise the proteolytic enzyme that described microbiobacterial agent fermentation produces in the organic liquor described in the present embodiment, lipase, α-amylase, amino acid.
Matrix described in the present embodiment comprises chicken manure or cow dung, brown coal, ground phosphate rock, medical stone, and the ratio of weight and number of described chicken manure or cow dung, brown coal, ground phosphate rock, medical stone is:
Chicken manure described in the present embodiment or cow dung are through 200～400 ℃ of high-temperature sterilizations; Described brown coal contain 20% humic acid; Described ground phosphate rock, medical stone are all more than 300 orders.
Embodiment 2
A kind of preparation method of complex microorganism rhzomorph alkaline land modifying agent, the manufacturing process steps of described modifying agent is as follows:
Described complex microorganism rhzomorph alkaline land modifying agent is obtained by 3 bacillus subtilis (Bacillus subtilis) and 1 strain bacillus megaterium (Bacillus megaterium) fermentation;
Described 3 bacillus subtilis are subtilis CGMCC 7296, subtilis CGMCC 7297, subtilis CGMCC 7298; Described bacillus megaterium is CGMCC 7337.
1) bacterial classification is prepared
With described 3 strain millet straw genus bacillus and the respectively separately activation on the ordinary broth inclined-plane of 1 strain bacillus megaterium, the bacterial classification after obtaining activating; Bacterial classification after the described activation is transferred to respectively in the flat bottle, cultivates 24h, make respectively No. 1 seed suspension, No. 2 seed suspension, No. 3 seed suspension, No. 4 seed suspension with 0.1 peptone aseptic aqueous solution for 28～30 ℃;
2) cultivation of seeding tank
Described 1～No. 3 seed suspension all is linked into the A seeding tank, and described No. 4 seed suspension are linked into the B seeding tank;
The prescription of substratum and ratio of weight and number thereof are in the A seeding tank:
The temperature of described A seeding tank is 28～34 ℃, and the pH of cultivation is 7.5～8.5;
The prescription of substratum and ratio of weight and number thereof are in the B seeding tank:
With described A, B seeding tank when the slack tank, respectively sterilization 45 minutes under 125 ℃/0.15mpa pressure, sterilization 30 minutes under 120 ℃/0.11mpa is cooled to 35 ℃ after the charging, 500 liters seeding tank constant volume 50%, described A, B seeding tank inoculate respectively 6～9 * 10
8Cfu; After the inoculation at 30～35 ℃, air flow 1: 1 (v: v), rotating speed 190～220r/min, after 7～9 hours, thalline reaches 1.0～1.5 * 10 in the bacterium liquid under the condition of tank pressure 0.05～0.08mpa
9/ ml, can culture transferring to the fermentor tank fermentation culture;
3) ferment tank
The culture medium prescription of fermentor tank and ratio of weight and number thereof are:
Described fermentor tank is under slack tank, and sterilization is 45 minutes under 125 ℃/0.15mpa pressure, sterilizes 30 minutes under 120 ℃/0.11mpa after the charging, is cooled to 35 ℃, 5000 liters fermentor tank constant volume 60%; Bacterium liquid after described A, the B seed tank culture is seeded in the described fermentor tank, and the leavening temperature of described fermentor tank is 28～34 ℃, and the pH of fermentation culture is 7.5～8.5; The air flow in 4 hours of fermenting is that (v: v), air flow increased to 1: 1 (v: v), fermentation total time is 36～38 hours, and thalline reaches 0.8～1.0 * 10 in the bacterium liquid after 4 hours in 1: 0.5
10/ ml, spore forming rate reaches 80%, the bacterium liquid after namely obtaining fermenting;
4) be adsorbed in matrix
Bacterium liquid and matrix after the described fermentation are stirred, according to 20～30L/m
3Be adsorbed in matrix, namely obtain described complex microorganism rhzomorph alkaline land modifying agent.Described matrix is the mixture of chicken manure or cow dung, brown coal, ground phosphate rock, medical stone, and the ratio of weight and number of described chicken manure or cow dung, brown coal, ground phosphate rock, medical stone is:
Further, described modifying agent requires to be not less than 5 * 10 through fungal counting
9/ g.
Embodiment 3
The present embodiment is the improvement on the basis of embodiment 2, and the part identical with embodiment 2 contents repeats no more.
The preparation method of the complex microorganism rhzomorph alkaline land modifying agent of the present embodiment, the manufacturing process steps of described modifying agent is as follows:
2) cultivation of seeding tank
The prescription of substratum and ratio of weight and number thereof are in the A seeding tank:
The temperature of described A seeding tank is 34 ℃, and the pH of cultivation is 7.5;
The prescription of substratum and ratio of weight and number thereof are in the B seeding tank:
3) ferment tank
The culture medium prescription of fermentor tank and ratio of weight and number thereof are:
4) be adsorbed in matrix
Bacterium liquid and matrix after the described fermentation are stirred, according to 20～30L/m
3Be adsorbed in matrix, namely obtain described complex microorganism rhzomorph alkaline land modifying agent.
Matrix described in the present embodiment comprises chicken manure or cow dung, brown coal, ground phosphate rock, medical stone, and the ratio of weight and number of described chicken manure or cow dung, brown coal, ground phosphate rock, medical stone is:
When the described complex microorganism rhzomorph of the present embodiment alkaline land modifying agent can be applicable to the rice transplanting of paddy rice saltings, band was fertile, the band medicine, carry disease germs and base fertilizer, dressing of turnning green stage use the same period, and consumption is 25kg/ mu.
Embodiment 4
The present embodiment is the improvement on the basis of embodiment 2, and the part identical with embodiment 2 contents repeats no more.
The preparation method of the complex microorganism rhzomorph alkaline land modifying agent of the present embodiment, the manufacturing process steps of described modifying agent is as follows:
2) cultivation of seeding tank
The prescription of substratum and ratio of weight and number thereof are in the A seeding tank:
The temperature of described A seeding tank is 34 ℃, and the pH of cultivation is 7.5;
The prescription of substratum and ratio of weight and number thereof are in the B seeding tank:
The cultivation pH of described A seeding tank is 7.5;
3) ferment tank
The culture medium prescription of fermentor tank and ratio of weight and number thereof are:
The temperature of described fermentor tank is 35 ℃, and the pH of cultivation is 7.5;
When the described complex microorganism rhzomorph of the present embodiment alkaline land modifying agent can be applicable to the rice transplanting of paddy rice saltings, band was fertile, the band medicine, carry disease germs and base fertilizer, dressing of turnning green stage use the same period, and consumption is 25kg/ mu.
Embodiment 5
The present embodiment is the improvement on the basis of embodiment 2, and the part identical with embodiment 2 contents repeats no more.
The preparation method of the complex microorganism rhzomorph alkaline land modifying agent of the present embodiment, the manufacturing process steps of described modifying agent is as follows:
2) cultivation of seeding tank
The prescription of substratum and ratio of weight and number thereof are in the A seeding tank:
The temperature of described A seeding tank is 34 ℃, and the pH of cultivation is 7.5;
The prescription of substratum and ratio of weight and number thereof are in the B seeding tank:
The cultivation pH of described A seeding tank is 7.5;
3) ferment tank
The culture medium prescription of fermentor tank and ratio of weight and number thereof are:
The temperature of described fermentor tank is 35 ℃, and the pH of cultivation is 7.5;
4) be adsorbed in matrix
Bacterium liquid and matrix after the described fermentation are stirred, according to 20～30L/m
3Be adsorbed in matrix, namely obtain described complex microorganism rhzomorph alkaline land modifying agent.
Further, described modifying agent requires to be not less than 5 * 10 through fungal counting
9/ g, the lipase preparation is not less than 50,000 units, and alpha Amylase preparation is not less than 20,000 units, and protease preparation is not less than 50,000 units.The examination criteria that relates to comprises: microbial fertilizer NY 227-94, lipase preparation GBT 23535-220, α-amylase QB 1805.1-1993 processed, protein formulation GBT 23527-2009.
When the described complex microorganism rhzomorph of the present embodiment alkaline land modifying agent can be applicable to the rice transplanting of paddy rice saltings, band was fertile, the band medicine, carry disease germs and base fertilizer, dressing of turnning green stage use the same period, and consumption is 25kg/ mu.
Embodiment 6
The present embodiment is the application of embodiment 2, embodiment 2 described complex microorganism rhzomorph alkaline land modifying agents are applied to Jianping township, Baicheng City in Jilin Province Zhenlai County, moderate saltings (total salt quantity is 0.3～0.6%) per hectare is used alkaline land modifying agent 250kg, the element Chemical Mixed Fertilizer that conventional fertilizer application selects border Chemical Group company limited of Sino-U.S. Sa Ke rich nations to produce, adjust nitrogen, phosphorus, the potassium active constituent content is respectively 22,12,15, every mu of consumption is 40kg, 10 cubes of fertilizers, described complex microorganism rhzomorph alkaline land modifying agent 300kg, wherein base fertilizer 200kg is turned green and is used 100kg.After using described complex microorganism rhzomorph alkaline land modifying agent, rice striking root is fast, anti-low temperature, and well developed root system, root are many in vain, and be resistant to lodging, and grouting is fast, and full grains shift to an earlier date 5～7 days maturations, and October, rice yield was 690kg/ mu.
Contrast: 1 hectare in rice field, heavy salinized ground, per hectare is used alkaline land modifying agent 350kg, and conventional fertilizer application is selected the element Chemical Mixed Fertilizer, adjustment nitrogen, phosphorus, potassium active constituent content are respectively 22,12,15, every mu of consumption is 40kg, 10 cubes of fertilizers, and October, rice yield was 480kg/ mu.
Find through contrast, paddy rice is after using described complex microorganism rhzomorph alkaline land modifying agent, and the increasing production of rice effect is remarkable.
Embodiment 7
The present embodiment is the application of embodiment 2, embodiment 2 described complex microorganism rhzomorph alkaline land modifying agents are applied to Jianping township, Baicheng City in Jilin Province Zhenlai County, 5 hectares in (total salt quantity is 0.6～0.9%) rice field, heavy salinized ground, per hectare is used alkaline land modifying agent 350kg, the element Chemical Mixed Fertilizer that conventional fertilizer application selects border Chemical Group company limited of Sino-U.S. Sa Ke rich nations to produce, adjust nitrogen, phosphorus, the potassium active constituent content is respectively 22,12,15, every mu of consumption is 40kg, 10 cubes of fertilizers, described complex microorganism rhzomorph alkaline land modifying agent 250kg, wherein base fertilizer 150kg is turned green and is used 100kg.After using described complex microorganism rhzomorph alkaline land modifying agent, rice striking root is fast, anti-low temperature, and well developed root system, root are many in vain, and be resistant to lodging, and grouting is fast, and full grains shift to an earlier date 5～7 days maturations, and October, rice yield was 670kg/ mu.
Contrast: 1 hectare in rice field, heavy salinized ground, per hectare is used alkaline land modifying agent 350kg, and conventional fertilizer application is selected the element Chemical Mixed Fertilizer, adjustment nitrogen, phosphorus, potassium active constituent content are respectively 22,12,15, every mu of consumption is 40kg, 10 cubes of fertilizers, and October, rice yield was 420kg/ mu.
Find through contrast, paddy rice is after using described complex microorganism rhzomorph alkaline land modifying agent, and the increasing production of rice effect is remarkable.
Embodiment 8
The present embodiment is the application of embodiment 2, embodiment 2 described complex microorganism rhzomorph alkaline land modifying agents are applied to Jianping township, Baicheng City in Jilin Province Zhenlai County, 5 hectares in slight alkaline land (total salt quantity is 0.1～0.3%) rice field, per hectare is used alkaline land modifying agent 150kg, the element Chemical Mixed Fertilizer that conventional fertilizer application selects border Chemical Group company limited of Sino-U.S. Sa Ke rich nations to produce, adjust nitrogen, phosphorus, the potassium active constituent content is respectively 22,12,15, every mu of consumption is 40kg, 10 cubes of fertilizers, described complex microorganism rhzomorph alkaline land modifying agent 200kg, wherein base fertilizer 100kg is turned green and is used 100kg.After using described complex microorganism rhzomorph alkaline land modifying agent, rice striking root is fast, anti-low temperature, and well developed root system, root are many in vain, and be resistant to lodging, and grouting is fast, and full grains shift to an earlier date 5～7 days maturations, and October, rice yield was 680kg/ mu.
Contrast: 1 hectare in rice field, heavy salinized ground, per hectare is used alkaline land modifying agent 350kg, and conventional fertilizer application is selected the element Chemical Mixed Fertilizer, adjustment nitrogen, phosphorus, potassium active constituent content are respectively 22,12,15, every mu of consumption is 40kg, 10 cubes of fertilizers, and October, rice yield was 560kg/ mu.
Find through contrast, paddy rice is after using described complex microorganism rhzomorph alkaline land modifying agent, and the increasing production of rice effect is remarkable.
Preservation information:
Claims (9)
1. a complex microorganism rhzomorph alkaline land modifying agent is characterized in that, described this good agent comprises microbiobacterial agent, organic liquor and matrix.
2. complex microorganism rhzomorph alkaline land modifying agent according to claim 1, it is characterized in that, described microbiobacterial agent comprises subtilis (Bacillus subtilis) and bacillus megaterium (Bacillus megaterium), and the ratio of weight and number of described subtilis and bacillus megaterium is:
Subtilis 60～80
Bacillus megaterium 20～40;
Optimum ratio of weight and number is:
Subtilis 70
Bacillus megaterium 30.
3. complex microorganism rhzomorph alkaline land modifying agent according to claim 2 is characterized in that, described subtilis is the mixture of subtilis CGMCC 7296, subtilis CGMCC 7297, subtilis CGMCC 7298; Described bacillus megaterium is CGMCC 7337.
4. complex microorganism rhzomorph alkaline land modifying agent according to claim 1 is characterized in that, comprises the proteolytic enzyme that described microbiobacterial agent fermentation produces in the described organic liquor, lipase, α-amylase, amino acid.
5. complex microorganism rhzomorph alkaline land modifying agent according to claim 1 is characterized in that, described matrix comprises chicken manure or cow dung, brown coal, ground phosphate rock, medical stone, and the ratio of weight and number of described chicken manure or cow dung, brown coal, ground phosphate rock, medical stone is:
6. complex microorganism rhzomorph alkaline land modifying agent according to claim 5 is characterized in that, described chicken manure or cow dung are through 200～400 ℃ of high-temperature sterilizations; Described brown coal contain 20% humic acid; Described ground phosphate rock, medical stone are all more than 300 orders.
7. the preparation method of a complex microorganism rhzomorph alkaline land modifying agent is characterized in that, the manufacturing process steps of described modifying agent is as follows:
Described complex microorganism rhzomorph alkaline land modifying agent is obtained by 3 bacillus subtilis (Bacillus subtilis) and 1 strain bacillus megaterium (Bacillus megaterium) fermentation;
Described 3 bacillus subtilis are subtilis CGMCC 7296, subtilis CGMCC 7297, subtilis CGMCC 7298; Described bacillus megaterium is CGMCC 7337;
1) bacterial classification is prepared
With described 3 strain millet straw genus bacillus and the respectively separately activation on the ordinary broth inclined-plane of 1 strain bacillus megaterium, the bacterial classification after obtaining activating; Bacterial classification after the described activation is transferred to respectively in the flat bottle, cultivates 24h, make respectively No. 1 seed suspension, No. 2 seed suspension, No. 3 seed suspension, No. 4 seed suspension with 0.1 peptone aseptic aqueous solution for 28～30 ℃;
2) cultivation of seeding tank
Described 1～No. 3 seed suspension all is linked into the A seeding tank, and described No. 4 seed suspension are linked into the B seeding tank;
The prescription of substratum and ratio of weight and number thereof are in the A seeding tank:
The temperature of described A seeding tank is 28～34 ℃, and the pH of cultivation is 7.5～8.5;
The prescription of substratum and ratio of weight and number thereof are in the B seeding tank:
With described A, B seeding tank when the slack tank, respectively sterilization 45 minutes under 125 ℃/0.15mpa pressure, sterilization 30 minutes under 120 ℃/0.11mpa is cooled to 35 ℃ after the charging, 500 liters seeding tank constant volume 50%, described A, B seeding tank inoculate respectively 6～9 * 10
8Cfu, after the inoculation at 30～35 ℃, air flow 1: 1 (v: v), rotating speed 190～220r/min, after 7～9 hours, thalline reaches 1.0～1.5 * 10 in the bacterium liquid under the condition of tank pressure 0.05～0.08mpa
9/ ml, can culture transferring to the fermentor tank fermentation culture;
3) ferment tank
The culture medium prescription of fermentor tank and ratio of weight and number thereof are:
Described fermentor tank is under slack tank, and sterilization is 45 minutes under 125 ℃/0.15mpa pressure, sterilizes 30 minutes under 120 ℃/0.11mpa after the charging, is cooled to 35 ℃, 5000 liters fermentor tank constant volume 60%; Bacterium liquid after described A, the B seed tank culture is seeded in the described fermentor tank, and the leavening temperature of described fermentor tank is 28～34 ℃, and the pH of fermentation culture is 7.5～8.5; The air flow in 4 hours of fermenting is that (v: v), air flow increased to 1: 1 (v: v), fermentation total time is 36～38 hours, and thalline reaches 0.8～1.0 * 10 in the bacterium liquid after 4 hours in 1: 0.5
10/ ml, spore forming rate reaches 80%, the bacterium liquid after namely obtaining fermenting;
4) be adsorbed in matrix
Bacterium liquid and matrix after the described fermentation are stirred, according to 20～30L/m
3Be adsorbed in matrix, namely obtain described complex microorganism rhzomorph alkaline land modifying agent;
Described matrix comprises the mixture of chicken manure or cow dung, brown coal, ground phosphate rock, medical stone.
8. the preparation method of complex microorganism rhzomorph alkaline land modifying agent according to claim 7 is characterized in that, described modifying agent requires to be not less than 5 * 10 through fungal counting
9/ g.
9. complex microorganism rhzomorph alkaline land modifying agent according to claim 1 is characterized in that, when described modifying agent can be applicable to the rice transplanting of paddy rice saltings, band was fertile, the band medicine, carry disease germs and base fertilizer, dressing of turnning green stage use the same period, and consumption is 25kg/ mu.
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| CN104826861A (en) * | 2015-04-30 | 2015-08-12 | 付新民 | Conditioning agent for soil restoration and treatment |
| CN104860732A (en) * | 2015-04-14 | 2015-08-26 | 广东森度生态农业科技有限公司 | Microbial strain organic fertilizer for improving soil alkalinity |
| CN105154103A (en) * | 2015-09-12 | 2015-12-16 | 安徽养禾天生物科技有限公司 | Improver applied to improving saline-alkali soil of grapes |
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| CN105154103A (en) * | 2015-09-12 | 2015-12-16 | 安徽养禾天生物科技有限公司 | Improver applied to improving saline-alkali soil of grapes |
| CN105670642A (en) * | 2016-03-07 | 2016-06-15 | 广西生物之源环保科技有限公司 | Saline alkali soil improver and preparation method thereof |
| CN105670642B (en) * | 2016-03-07 | 2018-11-20 | 广西素安生物科技有限公司 | Saline-alkali soil conditioner and preparation method thereof |
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| CN111019660A (en) * | 2019-11-14 | 2020-04-17 | 南京农业大学 | Microbial soil conditioner and preparation method and application thereof |
| CN112624873A (en) * | 2021-01-15 | 2021-04-09 | 甘南县龙头岭有机肥有限公司 | Biological organic fertilizer for saline-alkali soil and saline-alkali soil treatment method |
| CN115287080A (en) * | 2022-07-26 | 2022-11-04 | 上海星垣纳米科技有限公司 | Saline-alkali soil improver containing modified nano carbon material and preparation method thereof |
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