CN115287080A - Saline-alkali soil improver containing modified nano carbon material and preparation method thereof - Google Patents
Saline-alkali soil improver containing modified nano carbon material and preparation method thereof Download PDFInfo
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- 239000002689 soil Substances 0.000 title claims abstract description 48
- 229910021392 nanocarbon Inorganic materials 0.000 title claims abstract description 46
- 239000003513 alkali Substances 0.000 title claims abstract description 44
- 239000003575 carbonaceous material Substances 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 57
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 57
- 239000002202 Polyethylene glycol Substances 0.000 claims abstract description 37
- 229920001223 polyethylene glycol Polymers 0.000 claims abstract description 37
- 244000005700 microbiome Species 0.000 claims abstract description 23
- 238000005660 chlorination reaction Methods 0.000 claims abstract description 17
- 230000020477 pH reduction Effects 0.000 claims abstract description 17
- 239000011159 matrix material Substances 0.000 claims abstract description 12
- 230000004048 modification Effects 0.000 claims abstract description 8
- 238000012986 modification Methods 0.000 claims abstract description 8
- 244000063299 Bacillus subtilis Species 0.000 claims description 43
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 43
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 33
- 210000003608 fece Anatomy 0.000 claims description 29
- 239000010871 livestock manure Substances 0.000 claims description 24
- FYSNRJHAOHDILO-UHFFFAOYSA-N thionyl chloride Chemical compound ClS(Cl)=O FYSNRJHAOHDILO-UHFFFAOYSA-N 0.000 claims description 22
- 241000194107 Bacillus megaterium Species 0.000 claims description 15
- 239000000758 substrate Substances 0.000 claims description 14
- 241000287828 Gallus gallus Species 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 12
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 11
- 239000011259 mixed solution Substances 0.000 claims description 11
- 239000012046 mixed solvent Substances 0.000 claims description 11
- 229910017604 nitric acid Inorganic materials 0.000 claims description 11
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 11
- 238000000034 method Methods 0.000 claims description 10
- 241001494479 Pecora Species 0.000 claims description 2
- 239000012299 nitrogen atmosphere Substances 0.000 claims description 2
- 238000010992 reflux Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 230000004083 survival effect Effects 0.000 abstract description 8
- 230000006872 improvement Effects 0.000 abstract description 4
- 239000003607 modifier Substances 0.000 abstract description 3
- 239000003895 organic fertilizer Substances 0.000 abstract description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 2
- 150000001721 carbon Chemical class 0.000 abstract 1
- 239000002086 nanomaterial Substances 0.000 abstract 1
- 239000003516 soil conditioner Substances 0.000 abstract 1
- 238000002156 mixing Methods 0.000 description 29
- 239000000203 mixture Substances 0.000 description 22
- 238000000855 fermentation Methods 0.000 description 21
- 230000004151 fermentation Effects 0.000 description 21
- 239000007788 liquid Substances 0.000 description 15
- 230000000813 microbial effect Effects 0.000 description 13
- 230000000052 comparative effect Effects 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 239000003337 fertilizer Substances 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 239000002068 microbial inoculum Substances 0.000 description 7
- 238000012545 processing Methods 0.000 description 7
- 230000000855 fungicidal effect Effects 0.000 description 4
- 239000000417 fungicide Substances 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241001052560 Thallis Species 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000007480 spreading Effects 0.000 description 2
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000003077 lignite Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 239000002367 phosphate rock Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
- C09K17/42—Inorganic compounds mixed with organic active ingredients, e.g. accelerators
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Soil Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Inorganic Chemistry (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a saline-alkali soil conditioner containing a modified nano carbon material and a preparation method thereof, belonging to the technical field of soil improvement. The modified carbon nanomaterial comprises the steps of carbon tube acidification treatment, chlorination treatment and polyethylene glycol modification treatment. The saline-alkali soil improver containing the modified nano carbon material takes the excrement containing the modified nano carbon material as a matrix, adsorbs various microorganisms, and the adsorption matrix is an organic fertilizer and can also be used as a carrier of the microorganisms, so that the saline-alkali soil can be obviously improved. According to the saline-alkali soil improver containing the modified nano carbon material, a small amount of the modified nano carbon material is added into the matrix, so that the survival rate of microorganisms is greatly improved with low cost. According to the saline-alkali soil modifier containing the modified nano carbon material, the modified nano carbon material is subjected to acidification treatment, chlorination treatment and polyethylene glycol modification treatment, so that the survival rate of adsorbed microorganisms is remarkably improved.
Description
Technical Field
The invention belongs to the technical field of soil improvement, and relates to a saline-alkali soil improver containing a modified nano carbon material and a preparation method thereof.
Background
The salinization of soil is a worldwide problem, and the area of severe salinization land is rapidly expanded due to excessive use of chemical fertilizers in China. The saline-alkali soil contains excessive saline-alkali components such as chloride, sulfate, bicarbonate and the like, which can cause deterioration of physical and chemical properties of the soil, enhancement of soil alkalinity and humus leaching loss. The common saline-alkali soil improvement method comprises the steps of applying an organic fertilizer, applying a chemical modifier, applying a microbial fertilizer fungicide, breeding salt-tolerant plants and the like.
The microbial fertilizer microbial inoculum improves soil components and nutritional environment of saline-alkali soil through the life activities of microbes, and is concerned with good effect. CN103320137A discloses a compound microbial inoculum saline-alkali soil improver, and a preparation method and an application thereof, the compound microbial inoculum saline-alkali soil improver comprises a microbial agent, an organic solution and a matrix, the microbial agent comprises bacillus subtilis and bacillus megaterium, the matrix is chicken manure or cow manure, lignite, ground phosphate rock and medical stone, and a microbial fermentation bacteria liquid of the compound microbial inoculum is adsorbed on an untreated matrix. The microbial fertilizer fungicide plays a role and has a direct relation with microbial activity, and the microbial fertilizer fungicide loses activity along with the prolonging of time in the process of selling and storing on a shelf.
Disclosure of Invention
Aiming at the problems, the invention provides a preparation method of a modified nano carbon material for saline-alkali soil improvement, and the obtained modified substrate is used for adsorbing a fermented microbial agent, so that the survival rate of microorganisms is greatly prolonged.
The invention discloses a preparation method of a saline-alkali soil improver containing a modified nano carbon material, wherein the modified nano carbon material comprises the steps of acidification treatment, chlorination treatment and polyethylene glycol modification treatment of a carbon tube.
In some preferred embodiments of the present invention, further comprising the step of preparing a matrix using the modified nanocarbon material and feces.
In some preferred embodiments of the present invention, further comprising the step of adsorbing the microorganism with the substrate.
In some preferred embodiments of the present invention, the carbon tubes are acidified for 12-18h by using a mixed solution of concentrated sulfuric acid and concentrated nitric acid in a volume ratio of 1 (1-3).
In some preferred embodiments of the present invention, the chlorination treatment is performed by refluxing acidified carbon tubes with a mixed solvent of N, N-dimethylformamide and thionyl chloride in a volume ratio of 1 (40-60) at 60-70 ℃ for 18-24h.
In some preferred embodiments of the present invention, the carbon tubes modified by the polyethylene glycol are chlorinated by adding polyethylene glycol in a weight ratio of 1 (5-7), and melting in a nitrogen atmosphere.
In some preferred embodiments of the present invention, the manure is selected from one or more of pig manure, chicken manure, cow manure, and sheep manure.
In some preferred embodiments of the present invention, the weight ratio of the modified nanocarbon material to the feces is 1: (80-200).
In some preferred embodiments of the invention, the microorganism is Bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and Bacillus megaterium CGMCC7337, preferably, the wet cell weight ratio of Bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and Bacillus megaterium CGMCC7337 is (1-3): (1-3): 1-5).
In some preferred embodiments of the invention, the weight ratio of the microorganism to the substrate is 1: (100-150).
In some preferred embodiments of the invention, the substrate on which the microorganisms are adsorbed is dried to less than 15%, preferably to less than 10% at room temperature.
In some preferred embodiments of the present invention, after the substrate adsorbs the microorganisms, the uniformity of the mixture is judged by automatic photographing and image recognition processing, and the saline-alkali soil improver can be obtained only after the mixture is fully mixed, which specifically comprises the following steps:
s01, sampling the mixed substrate adsorbing the microorganisms, uniformly spreading the substrate with the thickness of 1cm, and taking a picture; sampling n, wherein n is not less than 5;
s02, automatically taking a picture and automatically transmitting data to a processing module;
s03, the processing module calculates the net distance variation coefficient C according to the following formula var ：
Wherein P1, P2, P3 and P4 are respectively the sum of the net distances of four sides of the image,
clear distance and mean value, A i Represents the area of the i-th particle, 1 1i 、1 2i 、1 3i And 1 4i Respectively the distance between the ith particle in the image and the four sides of the image;
s04, if the clear distance variation coefficient is smaller than a threshold value, representing that the mixture is sufficient; and if the net distance variation coefficient is larger than or equal to the threshold value, continuing mixing, and repeating S01-S03 until the net distance variation coefficient is smaller than the threshold value.
The second aspect of the invention discloses the saline-alkali soil improver containing the modified nano carbon material, which is obtained by the preparation method in the first aspect.
The invention has the beneficial effects that:
1. the saline-alkali soil improver containing the modified nano carbon material takes the excrement containing the modified nano carbon material as a matrix, adsorbs various microorganisms, and the adsorption matrix is an organic fertilizer and can also be used as a carrier of the microorganisms, so that the saline-alkali soil can be obviously improved.
2. According to the saline-alkali soil improver containing the modified nano carbon material, a small amount of the modified nano carbon material is added into the matrix, so that the survival rate of microorganisms is greatly improved with low cost.
3. According to the saline-alkali soil modifier containing the modified nano carbon material, the modified nano carbon material is subjected to acidification treatment, chlorination treatment and polyethylene glycol modification treatment, so that the survival rate of adsorbed microorganisms is remarkably improved.
4. According to the preparation method of the saline-alkali soil improver containing the modified nano carbon material, the sufficient degree of mixing of the matrix adsorbing the microorganisms is judged through automatic photographing and image recognition processing, and the preparation method is high in speed and good in effect.
Detailed Description
The following embodiments of the present invention are provided by way of specific examples, and other advantages and effects of the present invention will be readily apparent to those skilled in the art from the disclosure herein. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
The diameter of the carbon tube is 10-25nm, and the length is 0.5-50um. The polyethylene glycol is polyethylene glycol with a hydroxyl group at one end and a double bond at the other end, and the component is about 1100.
Unless otherwise specified, the examples and comparative examples are parallel tests with the same components, component contents, preparation steps, preparation parameters.
Example 1
Preparation method of saline-alkali soil improver containing modified nano carbon material
(1) Adding the carbon tube into a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 1;
(2) Adding the carbon tube subjected to acidification treatment obtained in the step (1) into a mixed solvent of N, N-dimethylformamide and thionyl chloride with the volume ratio of 1;
(3) Adding polyethylene glycol into the carbon tube subjected to chlorination treatment obtained in the step (2) according to the weight ratio of 1;
(4) And (3) taking the carbon tube treated by the polyethylene glycol obtained in the step (3), and mixing the carbon tube with the polyethylene glycol according to the proportion of 1:100 weight percent of the mixture is added into the crushed chicken manure and mixed evenly for standby;
(5) And (3) mixing fermentation liquids of bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and bacillus megatherium CGMCC7337 fermented to the logarithmic phase in a liquid fermentation manner according to the weight ratio of the converted wet bacteria 2.
Example 2
Preparation method of saline-alkali soil improver containing modified nano carbon material
(1) Adding a carbon tube into a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 1;
(2) Adding the carbon tube subjected to acidification treatment obtained in the step (1) into a mixed solvent of N, N-dimethylformamide and thionyl chloride with the volume ratio of 1;
(3) Adding polyethylene glycol into the carbon tube subjected to chlorination treatment obtained in the step (2) according to the weight ratio of 1;
(4) And (4) taking the carbon tube treated by the polyethylene glycol obtained in the step (3), and mixing the carbon tube treated by the polyethylene glycol according to the proportion of 1: adding 100 weight parts of the mixture into the crushed chicken manure, and uniformly mixing the mixture for later use;
(5) And (3) mixing fermentation liquids of bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and bacillus megatherium CGMCC7337 fermented to the logarithmic phase in a liquid fermentation manner according to the weight ratio of the converted wet bacteria 2.
Example 3
Preparation method of saline-alkali soil improver containing modified nano carbon material
(1) Adding a carbon tube into a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 1;
(2) Adding the carbon tube subjected to acidification treatment obtained in the step (1) into a mixed solvent of N, N-dimethylformamide and thionyl chloride with the volume ratio of 1;
(3) Adding polyethylene glycol into the carbon tube subjected to chlorination treatment obtained in the step (2) according to the weight ratio of 1;
(4) And (3) taking the carbon tube treated by the polyethylene glycol obtained in the step (3), and mixing the carbon tube with the polyethylene glycol according to the proportion of 1: adding 100 weight parts of the mixture into the crushed chicken manure, and uniformly mixing the mixture for later use;
(5) And (3) mixing fermentation liquids of bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and bacillus megatherium CGMCC7337 fermented to the logarithmic phase in a liquid fermentation manner according to the weight ratio of the converted wet bacteria 2.
Example 4
Preparation method of saline-alkali soil improver containing modified nano carbon material
(1) Adding the carbon tube into a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 1;
(2) Adding the carbon tube subjected to acidification treatment obtained in the step (1) into a mixed solvent of N, N-dimethylformamide and thionyl chloride with the volume ratio of 1;
(3) Adding polyethylene glycol into the carbon tube subjected to chlorination treatment obtained in the step (2) according to the weight ratio of 1;
(4) And (3) taking the carbon tube treated by the polyethylene glycol obtained in the step (3), and mixing the carbon tube with the polyethylene glycol according to the proportion of 1:100 weight percent of the mixture is added into the crushed chicken manure and mixed evenly for standby;
(5) And (3) mixing fermentation liquids of bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and bacillus megatherium CGMCC7337 fermented to the logarithmic phase in a liquid fermentation manner according to the weight ratio of the converted wet bacteria 2.
Example 5
Preparation method of saline-alkali soil improver containing modified nano carbon material
(1) Adding a carbon tube into a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 1;
(2) Adding the carbon tube subjected to acidification treatment obtained in the step (1) into a mixed solvent of N, N-dimethylformamide and thionyl chloride with the volume ratio of 1;
(3) Adding polyethylene glycol into the carbon tube subjected to chlorination treatment obtained in the step (2) according to the weight ratio of 1;
(4) And (3) taking the carbon tube treated by the polyethylene glycol obtained in the step (3), and mixing the carbon tube with the polyethylene glycol according to the proportion of 1: adding 100 weight parts of the mixture into the crushed chicken manure, and uniformly mixing the mixture for later use;
(5) And (3) mixing fermentation liquids of bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and bacillus megatherium CGMCC7337 fermented to the logarithmic phase in a liquid fermentation manner according to the weight ratio of the converted wet bacteria 2.
Example 6
Preparation method of saline-alkali soil improver containing modified nano carbon material
(1) Adding the carbon tube into a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 1;
(2) Adding the carbon tube subjected to acidification treatment obtained in the step (1) into a mixed solvent of N, N-dimethylformamide and thionyl chloride with the volume ratio of 1;
(3) Adding polyethylene glycol into the carbon tube subjected to chlorination treatment obtained in the step (2) according to the weight ratio of 1;
(4) And (4) taking the carbon tube treated by the polyethylene glycol obtained in the step (3), and mixing the carbon tube treated by the polyethylene glycol according to the proportion of 1: adding 100 weight parts of the mixture into the crushed pig manure, and uniformly mixing for later use;
(5) And (3) mixing fermentation liquids of bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and bacillus megatherium CGMCC7337 fermented to the logarithmic growth phase in a liquid fermentation manner according to the weight ratio of the converted wet bacteria 2.
Example 7
The difference from the embodiment 1 is that after the substrate adsorbs the microorganisms, the uniformity of the mixture is judged through automatic photographing and image recognition processing, and the saline-alkali soil improver can be obtained only after the mixture is fully mixed, and the method specifically comprises the following steps:
s01, sampling the mixed substrate adsorbing the microorganisms, uniformly spreading the substrate with the thickness of 1cm, and taking a picture; sampling n, wherein n is not less than 5;
s02, automatically taking a picture and automatically transmitting data to a processing module;
s03, the processing module calculates the net distance variation coefficient C according to the following formula var ：
Wherein P1, P2, P3 and P4 are respectively the sum of the net distances of four sides of the image,
clear distance and mean value, A i Represents the area of the i-th particle, 1 1i 、1 2i 、1 3i And 1 4i Respectively the distance between the ith particle in the image according to the four sides of the image;
s04, if the clear distance variation coefficient is smaller than a threshold value, representing that the mixture is sufficient; if the net distance variation coefficient is larger than or equal to the threshold value, mixing is continued, and S01-S03 are repeated until the net distance variation coefficient is smaller than the threshold value.
The threshold value of the clear distance variation coefficient which is suitable for judging the fully mixed chicken manure fertilizer is 1.2 percent, and the threshold value of the pig manure is 1.3 percent. The threshold value ensures sufficient mixing and avoids unnecessary energy and time waste.
Comparative example 1
Preparation method of saline-alkali soil improver containing modified nano carbon material
(1) Adding a carbon tube into a mixed solvent of N, N-dimethylformamide and thionyl chloride with the volume ratio of 1;
(2) Adding polyethylene glycol into the carbon tube subjected to acidification treatment obtained in the step (1) according to the weight ratio of 1;
(3) And (3) taking the carbon tube treated by the polyethylene glycol obtained in the step (2), and mixing the carbon tube with the polyethylene glycol according to the proportion of 1: adding 100 weight parts of the mixture into the crushed chicken manure, and uniformly mixing the mixture for later use;
(4) And (3) mixing fermentation liquids of bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and bacillus megatherium CGMCC7337 fermented to the logarithmic growth phase in a liquid fermentation manner according to the weight ratio of the converted wet bacteria 2.
Comparative example 2
Preparation method of saline-alkali soil improver containing modified nano carbon material
(1) Adding a carbon tube into a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 1;
(2) Adding polyethylene glycol into the carbon tube subjected to acidification treatment obtained in the step (1) according to the weight ratio of 1;
(3) Taking the carbon tube treated by the acid obtained in the step (2), and carrying out the following steps of 1:100 weight percent of the mixture is added into the crushed chicken manure and mixed evenly for standby;
(4) Taking fermentation liquor of bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and bacillus megatherium CGMCC7337, which is fermented to the logarithmic phase, mixing the fermentation liquor according to the weight ratio of the converted wet thalli 2.
Comparative example 3
Preparation method of saline-alkali soil improver containing modified nano carbon material
(1) Adding the carbon tube into a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 1;
(2) Adding the carbon tube subjected to acidification treatment obtained in the step (1) into a mixed solvent of N, N-dimethylformamide and thionyl chloride with the volume ratio of 1;
(3) And (3) taking the carbon tube subjected to chlorination treatment in the step (2), and performing chlorination treatment according to the weight ratio of 1: adding 100 weight parts of the mixture into the crushed chicken manure, and uniformly mixing the mixture for later use;
(4) Taking fermentation liquor of bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and bacillus megatherium CGMCC7337, which is fermented to the logarithmic phase, mixing the fermentation liquor according to the weight ratio of the converted wet thalli 2.
Comparative example 4
Preparation method of saline-alkali soil improver containing modified nano carbon material
(1) Adding a carbon tube into a mixed solution of concentrated sulfuric acid and concentrated nitric acid with a volume ratio of 1;
(2) Adding the carbon tube subjected to acidification treatment obtained in the step (1) into a mixed solvent of N, N-dimethylformamide and thionyl chloride with the volume ratio of 1;
(3) Adding polyethylene glycol into the carbon tube subjected to chlorination treatment obtained in the step (2) according to the weight ratio of 1;
(4) And (3) taking the carbon tube treated by the polyethylene glycol obtained in the step (3), and mixing the carbon tube with the polyethylene glycol according to the proportion of 1: adding 100 weight parts of the mixture into the crushed chicken manure, and uniformly mixing the mixture for later use;
(5) And (3) taking fermentation liquor of the bacillus subtilis CGMCC7296, the bacillus subtilis CGMCC7297, the bacillus subtilis CGMCC7298 and the bacillus megatherium CGMCC7337, which is fermented to the logarithmic phase, mixing the fermentation liquor according to the weight ratio of (2).
Performance investigation: preservation experiment of microbial fertilizer fungicide
The microbial fertilizer inocula obtained in the examples and the comparative examples are taken, accelerated and simulated in a 45 ℃ environment and stored, samples are respectively taken in 1 week, 2 weeks, 3 weeks and 4 weeks (simulated 3 months, 6 months, 12 months and 24 months), the effective viable count is determined according to the dilution method of GB 20287-2006 agricultural microbial inoculum, the relative survival rate of the microorganisms is calculated by taking the microbial inoculum stored for 0 week (not stored) as a reference, the steps are repeated for 5 times, and the average value is taken. The results are shown in Table 1.
TABLE 1 microbial fertilizer microbial inoculum preservation microbial survival rate
The results show that the relative survival rates at 4 weeks for examples 1, 2 and 3 are comparable, with no significant difference. Examples 1-3 are significantly superior to example 4 and comparative example 1, illustrating the significant effect of carbon tube acidification treatment; examples 1-3 are significantly superior to examples 5 and comparative example 2, illustrating the significant effect of chlorination treatment of acidified carbon tubes; examples 1-3 are significantly superior to comparative example 3, illustrating the significant effect of polyethylene glycol modification; examples 1-3 are significantly superior to comparative example 4, illustrating the significant effect of water content. Example 6, which used a cow dung substrate, was similar to examples 1-3 with no significant differences.
While the preferred embodiments and examples of the present invention have been described in detail, the present invention is not limited to the embodiments and examples, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (10)
1. A preparation method of a saline-alkali soil improver containing a modified nano carbon material is characterized in that the modified nano carbon material comprises the steps of acidification treatment, chlorination treatment and polyethylene glycol modification treatment of a carbon tube.
2. The method for preparing a saline-alkali soil improver containing a modified nanocarbon material as claimed in claim 1, further comprising a step of preparing a substrate from the modified nanocarbon material and feces.
3. The method for producing a saline-alkali soil improver containing a modified nanocarbon material as claimed in claim 1 or 2, further comprising a step of adsorbing microorganisms with the substrate.
4. The method for preparing a saline-alkali soil improver containing a modified nanocarbon material as claimed in any one of claims 1 to 3, wherein the acidification treatment is carried out on the carbon tube for 12 to 18 hours by using a mixed solution of concentrated sulfuric acid and concentrated nitric acid in a volume ratio of 1 (1-3).
5. The method for preparing a saline-alkali soil improver containing a modified nanocarbon material as claimed in any one of claims 1 to 4, wherein the chlorination treatment is carried out by refluxing acidified carbon tubes in a mixed solvent of N, N-dimethylformamide and thionyl chloride in a volume ratio of 1 (40-60) at 60-70 ℃ for 18-24 hours.
6. The method for preparing a saline-alkali soil improver containing a modified nanocarbon material as claimed in any one of claims 1 to 5, wherein the carbon tubes subjected to the modification treatment by polyethylene glycol are chlorinated and melted in a nitrogen atmosphere by adding polyethylene glycol in a weight ratio of 1 (5-7).
7. The method for preparing the saline-alkali soil improver containing the modified nanocarbon material as claimed in any one of claims 1 to 6, wherein the manure is one or more selected from pig manure, chicken manure, cow manure and sheep manure;
and/or the weight ratio of the modified nano carbon material to the feces is 1: (80-200).
8. The method for preparing the saline-alkali soil improver containing the modified nanocarbon material as claimed in any one of claims 1 to 7, wherein the microorganism is Bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and Bacillus megaterium CGMCC7337, preferably, the wet cell weight ratio of Bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, bacillus subtilis CGMCC7298 and Bacillus megaterium CGMCC7337 is (1-3): 1-5;
and/or, the weight ratio of the microorganism to the matrix is 1: (100-150).
9. The method for preparing a saline-alkali soil improver containing a modified nanocarbon material as claimed in any one of claims 1 to 8, wherein the substrate adsorbing the microorganisms is dried at room temperature to 15% or less, preferably to 10% or less.
10. A saline-alkali soil improver containing a modified nanocarbon material, which is obtained by the production method according to any one of claims 1 to 9.
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| CN103320137A (en) * | 2013-04-03 | 2013-09-25 | 清华大学 | Composite microbiological bacteriocin saline-alkali soil improver, and preparation method and applications thereof |
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| CN110423166A (en) * | 2019-09-05 | 2019-11-08 | 中国水稻研究所 | A kind of rice microbial fertilizer special of alkaline land improving |
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| CN114350229A (en) * | 2022-01-14 | 2022-04-15 | 深圳市朗迈新材料科技有限公司 | Graphene modified epoxy resin floor paint and preparation method thereof |
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| CN103320137A (en) * | 2013-04-03 | 2013-09-25 | 清华大学 | Composite microbiological bacteriocin saline-alkali soil improver, and preparation method and applications thereof |
| CN109851449A (en) * | 2019-01-15 | 2019-06-07 | 山西中盐大化肥业有限公司 | A kind of activated carbon biological organic fertilizer and preparation method thereof |
| CN110423166A (en) * | 2019-09-05 | 2019-11-08 | 中国水稻研究所 | A kind of rice microbial fertilizer special of alkaline land improving |
| CN113337296A (en) * | 2021-07-02 | 2021-09-03 | 上海星垣纳米科技有限公司 | Method for preparing saline-alkali soil improver by using nano carbon material |
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