CN103288560B - A kind of biological rhzomorph and middle trace element are in conjunction with the method for administering salt-soda soil - Google Patents

A kind of biological rhzomorph and middle trace element are in conjunction with the method for administering salt-soda soil Download PDF

Info

Publication number
CN103288560B
CN103288560B CN201310114402.XA CN201310114402A CN103288560B CN 103288560 B CN103288560 B CN 103288560B CN 201310114402 A CN201310114402 A CN 201310114402A CN 103288560 B CN103288560 B CN 103288560B
Authority
CN
China
Prior art keywords
rhzomorph
salt
soda soil
bacillus subtilis
agent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201310114402.XA
Other languages
Chinese (zh)
Other versions
CN103288560A (en
Inventor
王楠
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201310114402.XA priority Critical patent/CN103288560B/en
Publication of CN103288560A publication Critical patent/CN103288560A/en
Application granted granted Critical
Publication of CN103288560B publication Critical patent/CN103288560B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Fertilizers (AREA)
  • Soil Conditioners And Soil-Stabilizing Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a kind of biological rhzomorph and middle trace element in conjunction with the method for administering salt-soda soil. wherein, relate to a kind of complex microorganism rhzomorph salt-soda soil seedling protecting agent, comprise the fertile alkaline land modifying agent of biological rhzomorph and middle element. described microorganism rhzomorph is again by microbial bacterial agent, organic liquor and matrix composition, does described microbial bacterial agent specifically comprise bacillus subtilis (Bacillus? and bacillus megaterium (Bacillus subtilis)? megaterium), by fermenting, described microorganism rhzomorph can generate protease, lipase, AMS, the nutriments such as amino acid, the acid-base value of adjustable soil, reduce saline and alkaline toxic action, regulate rice root microbial environment, accelerate nitrogen, phosphorus, the conversion of potassium and middle trace element, accelerate root growth, extraneous root, send out seedling and strong seedling, for increasing production of rice is laid a good foundation.

Description

A kind of biological rhzomorph and middle trace element are in conjunction with the method for administering salt-soda soil
Technical field
The present invention relates to bio-bacterial manure technology and Saline-alkali Field Control field, particularly a kind of biological rhzomorph and middle trace are firstElement is in conjunction with the method for administering salt-soda soil.
Background technology
Ecology of alkali saline land reparation and improvement are engineerings of benefiting the nation and the people, and both can increase cultivated area, excavate agriculturalPotentiality, Guarantee Grain Production, is more conducive to the development of agricultural industry. Be conducive to expand foodstuff planting area, ensure having of grainEffect is supplied with, and is obtaining good achievement, in areas such as Chinese Inner Mongol, Jilin aspect Saline-alkali Field Control aspect Saline-alkali Field ControlPatent also has a lot, for example: Chinese patent CN101481281A (applying date: on February 3rd, 2009, application number:200910066498.0, applicant: Xinshiji Agriculture Science-Technology Co., Ltd., Jilin), Chinese patent CN100482064C (applicationDay: on September 14th, 2007, application number: 20071012790.9, applicant: Tsing-Hua University) utilize in coal desulfurize agent gypsumCalcium ion will cation replacements such as the sodium ions in soil colloid out, form calcium colloid and organic and inorganic complex, changesBecome soil aggregate, reduced the basicity of soil, improved soil permeability. The effect of Sodium Bicarbonate Soil Management agent is integratedThe physical chemistry functions such as chemistry salify, complexing, neutralization, ion-exchange, desorption are attached, passivation and fixing and the anti-accumulation of salt in the surface soil and breakdown of emulsion areOne. Aspect alkaline land improving, all obtain regulation effect. But, to the reparation of soil microenvironment, remove paddy riceThe yin, yang ion that root system is harmful, solubility Na+Ion does not well solve.
The main chloride in salt-soda soil or the higher salinization soil of sulfate, soil is alkalescence, but pH value is not necessarily veryHigh. Alkaline earth refers to carbonate containing or heavy phosphatic soil, and pH value is higher, and soil is alkalescence, and the content of organic matter of saline-alkali soil is few,Soil fertility is low, and physicochemical character is poor, and the yin, yang ion harmful to crop is many, has a strong impact on the crop short seedling of taking root, and makes grain yieldLowly.
Summary of the invention
The object of the invention is, according to the defect of above-described salt-soda soil seedling protecting agent, provides a kind of composite microbial bacteriaKeeping a full stand of seedings in element salt-soda soil, thereby provides a kind of biological rhzomorph and middle trace element in conjunction with the method for administering salt-soda soil. Utilize micro-lifeThe repair function of thing to soil, improvement soil environment, decomposes harmful substance and corrupt substance in soil, regulates the molecule of soilStructure, eliminates soil half, chesson, maintains soil micro-ecosystem balance, makes fertile soil.
Above-mentioned purpose of the present invention will be further described and be embodied by following detailed description:
A kind of complex microorganism rhzomorph salt-soda soil seedling protecting agent, described seedling protecting agent comprise biological rhzomorph and middle element fertilizer saline and alkalineGround modifying agent.
Further, described biological rhzomorph comprises microbial bacterial agent, organic liquor and matrix.
Further, described microbial bacterial agent comprises bacillus subtilis (Bacillussubtilis) and huge budSpore bacillus (Bacillusmegaterium), described bacillus subtilis and the ratio of weight and number of bacillus megaterium are:
Bacillus subtilis 60~80
Bacillus megaterium 20~40;
Optimum ratio of weight and number is:
Bacillus subtilis 70
Bacillus megaterium 30;
Further, described bacillus subtilis is bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, the mixture of bacillus subtilis CGMCC7298; Described bacillus megaterium preserving number is CGMCC7337.
Further, described organic liquor comprises the protease that described microbial bacterial agent fermentation produces, lipase, and α-Amylase, amino acid.
Further, described matrix comprises chicken manure or cow dung, brown coal, ground phosphate rock, medical stone, described chicken manure or cow dung,The ratio of weight and number of brown coal, ground phosphate rock, medical stone is:
Further, described chicken manure or cow dung are through 200~400 DEG C of high-temperature sterilizations; Described brown coal contain 20% corruptionGrow acid; Described ground phosphate rock, medical stone are all more than 300 orders.
Further, the fertile alkaline land modifying agent of described middle element comprise calcium hydroxide, magnesium sulfate, zinc sulfate, copper sulphate,Ferric sulfate, manganese sulfate, potassium dihydrogen phosphate and amino acid, described calcium hydroxide, magnesium sulfate, zinc sulfate, copper sulphate, ferric sulfate, sulphurAcid manganese, potassium dihydrogen phosphate and amino acid whose ratio of weight and number are:
Further, the manufacturing process steps of described biological rhzomorph is as follows:
Described biological rhzomorph is by 3 bacillus subtilis (Bacillussubtilis) and 1 strain bacillus megaterium(Bacillusmegaterium) fermentation obtains;
Described 3 bacillus subtilis are bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, witheredGrass bacillus CGMCC7298; Described bacillus megaterium preserving number is CGMCC7337.
1) bacterial classification is prepared
By 3 described strain millet straw bacillus and the activation separately on ordinary broth inclined-plane respectively of 1 strain bacillus megaterium,Obtain the bacterial classification after activation; Bacterial classification after described activation is transferred to respectively in flat bottle, cultivates 24h, use 0.1 albumen for 28~30 DEG CPeptone aseptic aqueous solution is made respectively No. 1 seed suspension, No. 2 seed suspension, No. 3 seed suspension, No. 4 seed suspension;
2) cultivation of seeding tank
1~No. 3 described seed suspension is all linked into A seeding tank, and No. 4 described seed suspension are linked into B kindSub-tank;
In A seeding tank, the formula of culture medium and ratio of weight and number thereof are:
The temperature of described A seeding tank is 28~34 DEG C, and the pH of cultivation is 7.5~8.5;
In B seeding tank, the formula of culture medium and ratio of weight and number thereof are:
By described A, B seeding tank when the slack tank, sterilizing 45 minutes under 125 DEG C/0.15mpa pressure respectively, after chargingSterilizing 30 minutes under 120 DEG C/0.11mpa, is cooled to 35 DEG C, the seeding tank constant volume 50% of 500 liters, and described A, B seeding tank are respectivelyInoculation 6~9 × 108Cfu, after inoculation at 30~35 DEG C, throughput 1: 1 (v: v), rotating speed 190~220r/min, tank pressure 0.05~Under the condition of 0.08mpa, after 7~9 hours, in bacterium liquid, thalline reaches 1.0~1.5 × 109/ ml can send out by culture transferring to fermentation tankFerment is cultivated;
3) ferment tank
The culture medium prescription of fermentation tank and ratio of weight and number thereof are:
Described fermentation tank under slack tank, sterilizing 45 minutes under 125 DEG C/0.15mpa pressure, after charging 120 DEG C/Sterilizing 30 minutes under 0.11mpa, is cooled to 35 DEG C, the fermentation tank constant volume 60% of 5000 liters; By after described A, B seed tank cultureBacterium liquid be seeded in described fermentation tank, the fermentation temperature of described fermentation tank is 28~34 DEG C, the pH of fermented and cultured is 7.5~8.5; The throughput fermenting in 4 hours be 1: 0.5 (v: v), the throughput (v: v), fermentation is at total time that is increased to 1: 1 after 4 hours36~38 hours, in bacterium liquid, thalline reached 0.8~1.0 × 1010/ ml, spore forming rate reaches 80%, obtains the bacterium after fermentationLiquid;
4) be adsorbed in matrix
Bacterium liquid and matrix after described fermentation are stirred, according to 20~30L/m3Be adsorbed in matrix, obtainDescribed complex microorganism rhzomorph salt-soda soil seedling protecting agent;
Described matrix is the mixture that comprises chicken manure or cow dung, brown coal, ground phosphate rock, medical stone.
Further, when described seedling protecting agent can be applicable to the rice transplanting of paddy rice salt-soda soil, be with fertilizer, band medicine, carry disease germs with base fertilizer, returnGreen manure uses the same period, and consumption is 15~35kg/ mu;
When application, first period fertile described middle element alkaline land modifying agent is imposed in salt-soda soil at base fertilizer, consumption is 5~15kg/ mu, then use described biological rhzomorph, consumption is 5~10kg/ mu; Turn green period, then use described biological bacteriaElement, consumption is 5~10kg/ mu.
The present invention's beneficial effect is compared to existing technology:
1, complex microorganism rhzomorph of the present invention salt-soda soil seedling protecting agent, has Salt And Alkali Tolerance microorganism numerous around rice rootReproductive growth, microorganism can constantly discharge protein, multivitamin, organic acid, amino acid, protease, sugar in growth courseChange enzyme, lipase, amylase etc., can eliminate fast the yin, yang ion harmful to rice root;
2, the beneficial microbe in the present invention breeding metabolic process in, sustainable secretion auxin isoreactivity material, energyEnough promote that paddy rice takes root, it is above that root is white, root capillary radical amount doubles, and fertilizer sucting ability strengthens, and stem is thick, Miao Zhuan, and root system is sent outReach, rice seedlings stalwartness, for increasing production of rice is laid a good foundation.
Detailed description of the invention
Further illustrate the present invention below in conjunction with embodiment, but protection scope of the present invention is not limited to embodiment.
Embodiment 1
A kind of complex microorganism rhzomorph salt-soda soil seedling protecting agent, described seedling protecting agent comprise biological rhzomorph and middle element fertilizer saline and alkalineGround modifying agent.
Further, described biological rhzomorph comprises microbial bacterial agent, organic liquor and matrix.
Further, described microbial bacterial agent comprises bacillus subtilis (Bacillussubtilis) and huge budSpore bacillus (Bacillusmegaterium), described bacillus subtilis and the ratio of weight and number of bacillus megaterium are:
Bacillus subtilis 60~80
Bacillus megaterium 20~40;
Optimum ratio of weight and number is:
Bacillus subtilis 70
Bacillus megaterium 30;
Further, described bacillus subtilis is bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, the mixture of bacillus subtilis CGMCC7298; Described bacillus megaterium preserving number is CGMCC7337.
Further, described organic liquor comprises the protease that described microbial bacterial agent fermentation produces, lipase, and α-Amylase, amino acid.
Further, described matrix comprises chicken manure or cow dung, brown coal, ground phosphate rock, medical stone, described chicken manure or cow dung,The ratio of weight and number of brown coal, ground phosphate rock, medical stone is:
Further, described chicken manure or cow dung are through 200~400 DEG C of high-temperature sterilizations; Described brown coal contain 20% corruptionGrow acid; Described ground phosphate rock, medical stone are all more than 300 orders.
Further, the fertile alkaline land modifying agent of described middle element comprise calcium hydroxide, magnesium sulfate, zinc sulfate, copper sulphate,Ferric sulfate, manganese sulfate, potassium dihydrogen phosphate and amino acid, described calcium hydroxide, magnesium sulfate, zinc sulfate, copper sulphate, ferric sulfate, sulphurAcid manganese, potassium dihydrogen phosphate and amino acid whose ratio of weight and number are:
Further, the manufacturing process steps of described biological rhzomorph is as follows:
Described biological rhzomorph is by 3 bacillus subtilis (Bacillussubtilis) and 1 strain bacillus megaterium(Bacillusmegaterium) fermentation obtains;
Described 3 bacillus subtilis are bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297, witheredGrass bacillus CGMCC7298; Described bacillus megaterium preserving number is CGMCC7337.
1) bacterial classification is prepared
By 3 described strain millet straw bacillus and the activation separately on ordinary broth inclined-plane respectively of 1 strain bacillus megaterium,Obtain the bacterial classification after activation; Bacterial classification after described activation is transferred to respectively in flat bottle, cultivates 24h, use 0.1 albumen for 28~30 DEG CPeptone aseptic aqueous solution is made respectively No. 1 seed suspension, No. 2 seed suspension, No. 3 seed suspension, No. 4 seed suspension;
2) cultivation of seeding tank
1~No. 3 described seed suspension is all linked into A seeding tank, and No. 4 described seed suspension are linked into B kindSub-tank;
In A seeding tank, the formula of culture medium and ratio of weight and number thereof are:
The temperature of described A seeding tank is 28~34 DEG C, and the pH of cultivation is 7.5~8.5;
In B seeding tank, the formula of culture medium and ratio of weight and number thereof are:
By described A, B seeding tank when the slack tank, sterilizing 45 minutes under 125 DEG C/0.15mpa pressure respectively, after chargingSterilizing 30 minutes under 120 DEG C/0.11mpa, is cooled to 35 DEG C, the seeding tank constant volume 50% of 500 liters, and described A, B seeding tank are respectivelyInoculation 6~9 × 108Cfu, after inoculation at 30~35 DEG C, throughput 1: 1 (v: v), rotating speed 190~220r/min, tank pressure 0.05~Under the condition of 0.08mpa, after 7~9 hours, in bacterium liquid, thalline reaches 1.0~1.5 × 109/ ml can send out by culture transferring to fermentation tankFerment is cultivated;
3) ferment tank
The culture medium prescription of fermentation tank and ratio of weight and number thereof are:
Described fermentation tank under slack tank, sterilizing 45 minutes under 125 DEG C/0.15mpa pressure, after charging 120 DEG C/Sterilizing 30 minutes under 0.11mpa, is cooled to 35 DEG C, the fermentation tank constant volume 60% of 5000 liters; By after described A, B seed tank cultureBacterium liquid be seeded in described fermentation tank, the fermentation temperature of described fermentation tank is 28~34 DEG C, the pH of fermented and cultured is 7.5~8.5; The throughput fermenting in 4 hours be 1: 0.5 (v: v), the throughput (v: v), fermentation is at total time that is increased to 1: 1 after 4 hours36~38 hours, in bacterium liquid, thalline reached 0.8~1.0 × 1010/ ml, spore forming rate reaches 80%, obtains the bacterium after fermentationLiquid;
4) be adsorbed in matrix
Bacterium liquid and matrix after described fermentation are stirred, according to 20~30L/m3Be adsorbed in matrix, obtainDescribed complex microorganism rhzomorph salt-soda soil seedling protecting agent;
Described matrix is the mixture that comprises chicken manure or cow dung, brown coal, ground phosphate rock, medical stone.
Further, described seedling protecting agent, through fungal counting, requires to be not less than 5 × 109/g。
Further, when described seedling protecting agent can be applicable to the rice transplanting of paddy rice salt-soda soil, be with fertilizer, band medicine, carry disease germs with base fertilizer, returnGreen manure uses the same period, and consumption is 15~35kg/ mu;
When application, first period fertile described middle element alkaline land modifying agent is imposed in salt-soda soil at base fertilizer, consumption is 5~15kg/ mu, then use described biological rhzomorph, consumption is 5~10kg/ mu; Turn green period, then use described biological bacteriaElement, consumption is 5~10kg/ mu.
Embodiment 2
The present embodiment is the improvement on the basis of embodiment 1, and the part identical with embodiment 1 content repeats no more.
Further, the fertile alkaline land modifying agent of described middle element comprise calcium hydroxide, magnesium sulfate, zinc sulfate, copper sulphate,Ferric sulfate, manganese sulfate, potassium dihydrogen phosphate and amino acid, described calcium hydroxide, magnesium sulfate, zinc sulfate, copper sulphate, ferric sulfate, sulphurAcid manganese, potassium dihydrogen phosphate and amino acid whose ratio of weight and number are:
The manufacturing process steps of the described biological rhzomorph of the present embodiment is as follows:
2) cultivation of seeding tank
In A seeding tank, the formula of culture medium and ratio of weight and number thereof are:
The temperature of described A seeding tank is 34 DEG C, and the pH of cultivation is 7.5;
In B seeding tank, the formula of culture medium and ratio of weight and number thereof are:
3) ferment tank
The culture medium prescription of fermentation tank and ratio of weight and number thereof are:
4) be adsorbed in matrix
Bacterium liquid and matrix after described fermentation are stirred, according to 20~30L/m3Be adsorbed in matrix, obtainDescribed complex microorganism rhzomorph salt-soda soil seedling protecting agent.
Matrix described in the present embodiment comprises chicken manure or cow dung, brown coal, ground phosphate rock, medical stone, described chicken manure or cow dung,The ratio of weight and number of brown coal, ground phosphate rock, medical stone is:
Embodiment 3
The present embodiment is the improvement on the basis of embodiment 1, and the part identical with embodiment 1 content repeats no more.
Further, the fertile alkaline land modifying agent of described middle element comprise calcium hydroxide, magnesium sulfate, zinc sulfate, copper sulphate,Ferric sulfate, manganese sulfate, potassium dihydrogen phosphate and amino acid, described calcium hydroxide, magnesium sulfate, zinc sulfate, copper sulphate, ferric sulfate, sulphurAcid manganese, potassium dihydrogen phosphate and amino acid whose ratio of weight and number are:
The manufacturing process steps of described biological rhzomorph is as follows:
2) cultivation of seeding tank
In A seeding tank, the formula of culture medium and ratio of weight and number thereof are:
The temperature of described A seeding tank is 34 DEG C, and the pH of cultivation is 7.5;
In B seeding tank, the formula of culture medium and ratio of weight and number thereof are:
The cultivation pH of described A seeding tank is 7.5;
3) ferment tank
The culture medium prescription of fermentation tank and ratio of weight and number thereof are:
The temperature of described fermentation tank is 35 DEG C, and the pH of cultivation is 7.5;
Embodiment 4
The present embodiment is the improvement on the basis of embodiment 1, and the part identical with embodiment 1 content repeats no more.
The manufacturing process steps of described biological rhzomorph is as follows:
2) cultivation of seeding tank
In A seeding tank, the formula of culture medium and ratio of weight and number thereof are:
The temperature of described A seeding tank is 34 DEG C, and the pH of cultivation is 7.5;
In B seeding tank, the formula of culture medium and ratio of weight and number thereof are:
The cultivation pH of described A seeding tank is 7.5;
3) ferment tank
The culture medium prescription of fermentation tank and ratio of weight and number thereof are:
The temperature of described fermentation tank is 35 DEG C, and the pH of cultivation is 7.5;
4) be adsorbed in matrix
Bacterium liquid and matrix after described fermentation are stirred, according to 20~30L/m3Be adsorbed in matrix, obtainDescribed biological rhzomorph.
Further, described biological rhzomorph, through fungal counting, requires to be not less than 5 × 109/ g, lipase preparation is not less than 5Ten thousand units, alpha Amylase preparation is not less than 20,000 units, and protease preparation is not less than 50,000 units. The examination criteria relating to comprises:Microbial manure NY227-94, lipase preparation GBT23535-220, AMS QB1805.1-1993 processed, protein systemAgent GBT23527-2009.
Embodiment 5
The present embodiment is the application of embodiment 1, by the complex microorganism rhzomorph salt-soda soil seedling protecting agent application described in embodiment 1In Jianping township, Zhenlai County, Baicheng City in Jilin Province, the fertile salt of element during moderate salt-soda soil (total salt quantity is 0.3~0.6%) per hectare is usedAlkali ground modifying agent 250kg, the three elements Chemical Mixed Fertilizer that conventional fertilizer application selects border Chemical Group Co., Ltd of Sa Ke rich nations of Sino-U.S. to produce,Adjustment nitrogen, phosphorus, potassium active constituent content are respectively 22,12,15, and every mu of consumption is 40kg, 10 cubes of fertilizers, described biologyRhzomorph 300kg, wherein base fertilizer 200kg, turns green and uses 100kg. Use after described complex microorganism rhzomorph salt-soda soil seedling protecting agent,Rice striking root is fast, anti-low temperature, and well developed root system, root is many in vain, resistant to lodging, and grouting is fast, full grains, 5~7 days maturations in advance, OctoberRice yield is 690kg/ mu.
Contrast: 1 hectare, rice field, heavy salinized ground, per hectare is used salt-soda soil seedling protecting agent 350kg, and conventional fertilizer application is selected ternaryElement Chemical Mixed Fertilizer, adjustment nitrogen, phosphorus, potassium active constituent content are respectively 22,12,15, and every mu of consumption is 40kg, 10 cubes of fertilizers,October, rice yield was 480kg/ mu.
Find through contrast, paddy rice is being used after described complex microorganism rhzomorph salt-soda soil seedling protecting agent, increasing production of rice effectSignificantly.
Embodiment 6
The present embodiment is the application of embodiment 1, by the complex microorganism rhzomorph salt-soda soil seedling protecting agent application described in embodiment 1In Jianping township, Zhenlai County, Baicheng City in Jilin Province, 5 hectares, (total salt quantity is 0.6~0.9%) rice field, heavy salinized ground, per hectare is usedThe fertile alkaline land modifying agent 350kg of middle element, conventional fertilizer application is selected three of border Chemical Group Co., Ltd of Sa Ke rich nations of Sino-U.S. productionElement Chemical Mixed Fertilizer, adjustment nitrogen, phosphorus, potassium active constituent content are respectively 22,12,15, and every mu of consumption is 40kg, and fertilizer 10 is verticalSide, described biological rhzomorph 250kg, wherein base fertilizer 150kg, turns green and uses 100kg. Use described complex microorganism rhzomorph saltAfter alkali ground seedling protecting agent, rice striking root is fast, anti-low temperature, and well developed root system, root is many in vain, resistant to lodging, and grouting is fast, full grains, in advance 5~7 days maturations, October, rice yield was 670kg/ mu.
Contrast: 1 hectare, rice field, heavy salinized ground, per hectare is used salt-soda soil seedling protecting agent 350kg, and conventional fertilizer application is selected ternaryElement Chemical Mixed Fertilizer, adjustment nitrogen, phosphorus, potassium active constituent content are respectively 22,12,15, and every mu of consumption is 40kg, 10 cubes of fertilizers,October, rice yield was 420kg/ mu.
Find through contrast, paddy rice is being used after described complex microorganism rhzomorph salt-soda soil seedling protecting agent, increasing production of rice effectSignificantly.
Embodiment 7
The present embodiment is the application of embodiment 1, by the complex microorganism rhzomorph salt-soda soil seedling protecting agent application described in embodiment 1In Jianping township, Zhenlai County, Baicheng City in Jilin Province, 5 hectares, slight alkaline land (total salt quantity is 0.1~0.3%) rice field, per hectare is usedThe fertile alkaline land modifying agent 150kg of middle element, conventional fertilizer application is selected three of border Chemical Group Co., Ltd of Sa Ke rich nations of Sino-U.S. productionElement Chemical Mixed Fertilizer, adjustment nitrogen, phosphorus, potassium active constituent content are respectively 22,12,15, and every mu of consumption is 40kg, and fertilizer 10 is verticalSide, described biological rhzomorph 200kg, wherein base fertilizer 100kg, turns green and uses 100kg. Use described complex microorganism rhzomorph saltAfter alkali ground seedling protecting agent, rice striking root is fast, anti-low temperature, and well developed root system, root is many in vain, resistant to lodging, and grouting is fast, full grains, in advance 5~7 days maturations, October, rice yield was 680kg/ mu.
Contrast: 1 hectare, rice field, heavy salinized ground, per hectare is used salt-soda soil seedling protecting agent 350kg, and conventional fertilizer application is selected ternaryElement Chemical Mixed Fertilizer, adjustment nitrogen, phosphorus, potassium active constituent content are respectively 22,12,15, and every mu of consumption is 40kg, 10 cubes of fertilizers,October, rice yield was 560kg/ mu.
Find through contrast, paddy rice is being used after described complex microorganism rhzomorph salt-soda soil seedling protecting agent, increasing production of rice effectSignificantly.Preservation information:

Claims (5)

1. a complex microorganism rhzomorph salt-soda soil seedling protecting agent, is characterized in that, described seedling protecting agent comprise biological rhzomorph and inThe fertile alkaline land modifying agent of element;
Described biological rhzomorph comprises microbial bacterial agent, organic liquor and matrix;
Described microbial bacterial agent comprises bacillus subtilis (Bacillussubtilis) and bacillus megaterium(Bacillusmegaterium), the ratio of weight and number of described bacillus subtilis and bacillus megaterium is:
Bacillus subtilis 60~80
Bacillus megaterium 20~40;
Further, described bacillus subtilis be bacillus subtilis CGMCC7296, bacillus subtilis CGMCC7297,The mixture of bacillus subtilis CGMCC7298; Described bacillus megaterium is CGMCC7337;
Described organic liquor comprises the protease that described microbial bacterial agent fermentation produces, lipase, AMS, aminoAcid;
The fertile alkaline land modifying agent of described middle element comprises calcium hydroxide, magnesium sulfate, zinc sulfate, copper sulphate, ferric sulfate, sulfuric acidManganese, potassium dihydrogen phosphate and amino acid, described calcium hydroxide, magnesium sulfate, zinc sulfate, copper sulphate, ferric sulfate, manganese sulfate, di(2-ethylhexyl)phosphateHydrogen potassium and amino acid whose ratio of weight and number are:
2. complex microorganism rhzomorph according to claim 1 salt-soda soil seedling protecting agent, is characterized in that, described withered grass gemmaBacillus and bacillus megaterium ratio of weight and number are:
Bacillus subtilis 70
Bacillus megaterium 30.
3. complex microorganism rhzomorph according to claim 1 salt-soda soil seedling protecting agent, is characterized in that, described matrix comprisesChicken manure or cow dung, brown coal, ground phosphate rock, medical stone, the ratio of weight and number of described chicken manure or cow dung, brown coal, ground phosphate rock, medical stoneFor:
4. complex microorganism rhzomorph according to claim 3 salt-soda soil seedling protecting agent, is characterized in that described chicken manure or oxExcrement is through 200~400 DEG C of high-temperature sterilizations; Described brown coal contain 20% humic acid; Described ground phosphate rock, medical stone are all at 300 ordersAbove.
5. complex microorganism rhzomorph according to claim 1 salt-soda soil seedling protecting agent, is characterized in that, described seedling protecting agent canWhile being applied to the rice transplanting of paddy rice salt-soda soil, band is fertile, band medicine, carry disease germs and base fertilizer, dressing of turnning green stage use the same period, and consumption is 15~35kg/ mu;When application, first period fertile described middle element alkaline land modifying agent is imposed in salt-soda soil at base fertilizer, consumption is 5~15kg/Mu, then use described biological rhzomorph, consumption is 5~10kg/ mu; Turn green period, then use described biological rhzomorph, consumption is5~10kg/ mu.
CN201310114402.XA 2013-04-03 2013-04-03 A kind of biological rhzomorph and middle trace element are in conjunction with the method for administering salt-soda soil Expired - Fee Related CN103288560B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310114402.XA CN103288560B (en) 2013-04-03 2013-04-03 A kind of biological rhzomorph and middle trace element are in conjunction with the method for administering salt-soda soil

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310114402.XA CN103288560B (en) 2013-04-03 2013-04-03 A kind of biological rhzomorph and middle trace element are in conjunction with the method for administering salt-soda soil

Publications (2)

Publication Number Publication Date
CN103288560A CN103288560A (en) 2013-09-11
CN103288560B true CN103288560B (en) 2016-05-18

Family

ID=49090165

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310114402.XA Expired - Fee Related CN103288560B (en) 2013-04-03 2013-04-03 A kind of biological rhzomorph and middle trace element are in conjunction with the method for administering salt-soda soil

Country Status (1)

Country Link
CN (1) CN103288560B (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103283338B (en) * 2013-07-03 2016-08-24 张维 A kind of method of salt-soda soil rice cultivation
CN103611724A (en) * 2013-11-07 2014-03-05 吉林牧田农业技术开发有限公司 Saline-alkali soil restoration agent
CN104928200B (en) * 2014-03-17 2018-01-19 青岛科技大学 There is bacillus marinus and the application of multiple functions to plant
CN104262046A (en) * 2014-09-12 2015-01-07 瑞昊(北京)环境工程有限公司 Fertilizer for saline-alkali soil and preparation method of fertilizer
CN104557279A (en) * 2014-12-22 2015-04-29 郭爱民 Seedling caring essence
CN104826861A (en) * 2015-04-30 2015-08-12 付新民 Conditioning agent for soil restoration and treatment
CN104892130A (en) * 2015-05-19 2015-09-09 潍坊友容实业有限公司 A microbial fertilizer for saline-alkali land and applications thereof in plantation of vitex rotundifolia
CN104876714A (en) * 2015-05-19 2015-09-02 潍坊友容实业有限公司 High-manganese bacterial fertilizer for saline-alkali soil and application thereof in Chinese soapberry planting
CN105777351B (en) * 2016-03-17 2020-02-14 浙江科技学院 Preparation method of pig manure fermentation-free low-cost organic fertilizer
CN106008101A (en) * 2016-05-25 2016-10-12 山西凯盛肥业集团有限公司 Composite microbial organic fertilizer for saline-alkali soil improvement and preparation method thereof
CN108048102B (en) * 2017-12-12 2020-12-11 中科绿洲(北京)生态工程技术有限公司 Microbial repairing agent for saline-alkali soil as well as preparation method and using method thereof
CN112189394A (en) * 2020-08-21 2021-01-08 中农海稻(深圳)生物科技有限公司 Three-dimensional improvement method for saline-alkali soil
CN112174718A (en) * 2020-09-29 2021-01-05 东北农业大学 Microbial preparation for promoting rooting and lodging resistance of rice and application thereof
CN112592222A (en) * 2020-11-05 2021-04-02 东北农业大学 Solid-state composite microorganism rooting agent for improving saline-alkali soil and application thereof
CN112624873A (en) * 2021-01-15 2021-04-09 甘南县龙头岭有机肥有限公司 Biological organic fertilizer for saline-alkali soil and saline-alkali soil treatment method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101544516A (en) * 2008-12-08 2009-09-30 上海绿乐生物科技有限公司 Production method of organic fertilization fertilizer
CN101781149A (en) * 2010-02-08 2010-07-21 张钧 Bio-organic compound fertilizer
CN101935248A (en) * 2009-09-21 2011-01-05 上海绿乐生物科技有限公司 Bentonite coated complex microbial inoculant as well as preparation method and application thereof
CN101935249A (en) * 2009-12-17 2011-01-05 上海绿乐生物科技有限公司 Granular type compound microbial bacterial agent as well as preparation method and application thereof
CN102531776A (en) * 2011-12-23 2012-07-04 于永波 Compound microbial fertilizer and method for producing same

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101544516A (en) * 2008-12-08 2009-09-30 上海绿乐生物科技有限公司 Production method of organic fertilization fertilizer
CN101935248A (en) * 2009-09-21 2011-01-05 上海绿乐生物科技有限公司 Bentonite coated complex microbial inoculant as well as preparation method and application thereof
CN101935249A (en) * 2009-12-17 2011-01-05 上海绿乐生物科技有限公司 Granular type compound microbial bacterial agent as well as preparation method and application thereof
CN101781149A (en) * 2010-02-08 2010-07-21 张钧 Bio-organic compound fertilizer
CN102531776A (en) * 2011-12-23 2012-07-04 于永波 Compound microbial fertilizer and method for producing same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
微生物菌剂对盐碱土理化和生物性状影响的研究;逄焕成等;《农业环境科学学报》;20090520;第28卷(第5期);第951-955页 *

Also Published As

Publication number Publication date
CN103288560A (en) 2013-09-11

Similar Documents

Publication Publication Date Title
CN103288560B (en) A kind of biological rhzomorph and middle trace element are in conjunction with the method for administering salt-soda soil
CN103320137B (en) A kind of composite microbiological bacteriocin saline-alkali soil improver and preparation method thereof and application
CN104609979B (en) A kind of microbial-bacterial fertilizer for improveing salt affected soil and preparation method thereof
CN106342432B (en) A method of promoting soil carbon sequestration in corn-wheat Rotation System
CN103922846B (en) Saline-alkali soil improvement method
CN103204732B (en) Composite microbial fertilizer containing polyglutamate high-producing strain, preparation method and application thereof
CN103910578B (en) Rooting nutritional type microbial fertilizer, and preparation method and applications thereof
CN101844944B (en) Total nutrient liquid microbial fertilizer
CN108410470B (en) Microbial soil conditioner for saline-alkali soil and preparation method thereof
CN104744112A (en) Compound foliar fertilizer and preparation method thereof
CN107793267A (en) One kind is for sulfate alkaline land improving microbial-bacterial fertilizer and preparation method thereof
CN103044157A (en) Amino acid guniting compound fertilizer and preparation method thereof
CN106747804A (en) A kind of Methylotrophic gemma bacillus agent and preparation method thereof
CN109496758A (en) A kind of disease is inoculated with reparation and the nursing method of tobacco field
CN102417412A (en) Humic acid microbial fertilizer and preparation method thereof
CN104478635A (en) Preparation method of soil conditioner
CN109485508A (en) A kind of control method of plant root rot
CN102503699A (en) Lysozyme soil sterilization deep ploughing-free anti-hardening root cultivation and protection microorganism fertilizer
CN108358695A (en) A kind of cabbage formula fertilizer special and preparation method thereof
CN108191538A (en) Composite microbiological fertilizer comprising urea phosphate and polyglutamic acid
CN110684683A (en) Bacillus amyloliquefaciens, microbial inoculum and application
CN106171111A (en) A kind of desulfurated plaster compounding application bacterial manure modification method to salt-soda soil
CN103351188A (en) Preparation method of agricultural organic fertilizer by treating pure straws as raw material
CN105175159A (en) Production method of rice seedling dry raising and strengthening biological medium and application thereof
CN108901755A (en) A kind of watermelon seedling culturing growth-promoting matrix and preparation method thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160518