CN108048102B - Microbial repairing agent for saline-alkali soil as well as preparation method and using method thereof - Google Patents
Microbial repairing agent for saline-alkali soil as well as preparation method and using method thereof Download PDFInfo
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- CN108048102B CN108048102B CN201711322780.1A CN201711322780A CN108048102B CN 108048102 B CN108048102 B CN 108048102B CN 201711322780 A CN201711322780 A CN 201711322780A CN 108048102 B CN108048102 B CN 108048102B
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
Abstract
The invention relates to a microbial repairing agent for saline-alkali soil, which comprises the following raw materials, by weight, 5-9 parts of ethylene-vinyl acetate copolymer, 12-16 parts of vinyl alcohol-ethylene copolymer, 11-19 parts of sodium alginate, 2-7 parts of calcium chloride, 35-45 parts of bacterial liquid and 5-9 parts of polypropylene glycol. The microbial repairing agent for saline-alkali soil improves the soil structure by using microbes, improves the metabolic activity of the microbes and achieves the effect of repairing the saline-alkali soil.
Description
Technical Field
The invention relates to the field of ecological restoration of saline-alkali soil, in particular to a microbial restoration agent for saline-alkali soil and a preparation method and a use method thereof.
Background
Saline-alkali soil is a general term for various saline and alkaline soils and salinized and alkalized soils of different degrees. At present, various ecological environment problems caused by soil salinization, such as water loss and soil erosion, land desertification, forest and grassland resource reduction, biodiversity reduction and the like become global problems.
Common saline-alkali soil treatment methods comprise a hydraulic engineering method, a chemical improvement method, a biological improvement method and the like. The hydraulic engineering method comprises a fresh water salt washing method, a salt discharging method for building a hidden pipe ditch and the like. However, the hydraulic engineering method requires sufficient high-quality water sources and good drainage systems to combine irrigation and drainage, so that the investment is huge, the maintenance cost is high, the treatment of saline drainage water is difficult, secondary salinization of soil and loss of mineral elements such as P, Fe, Mg and Zn necessary for some plants in the soil are easily caused, and once irrigation and salt washing are stopped, the soil can gradually return salt.
The chemical improvement method comprises applying gypsum, industrial waste residue, inorganic modifier, organic modifier and the like in the saline-alkali soil. Although the short-term effect of the chemical improvement method is remarkable, the cost is too high, the effect of the single chemical improvement agent is not good, and secondary pollution is easily caused.
The biological improvement method is a technique for repairing polluted soil by using microorganisms, namely, the special microorganisms are used to act on saline-alkali soil through mineralization, co-metabolism, biological concentration or accumulation and the like. However, due to high salinity and high alkalinity in the saline-alkali soil, after the microorganisms are applied to the saline-alkali soil, the living environment is severe, the metabolic activity of the microorganisms is reduced, and the capability of the microorganisms for repairing the saline-alkali soil is weakened.
Disclosure of Invention
The invention aims to provide a microbial repairing agent for saline-alkali soil, a preparation method and a use method thereof.
The above object of the present invention is achieved by the following technical solutions: the microbial repairing agent for saline-alkali soil is characterized by comprising, by weight, 5-9 parts of ethylene-vinyl acetate copolymer, 12-16 parts of vinyl alcohol-ethylene copolymer, 11-19 parts of sodium alginate, 2-7 parts of calcium chloride, 35-45 parts of bacterial liquid and 5-9 parts of polypropylene glycol.
Preferably, the average molecular weight of the ethylene-vinyl acetate copolymer is 1500-2500, and the average molecular weight of the vinyl alcohol-ethylene copolymer is 2000-3000.
Preferably, the bacterial liquid is a mixture of a substrate liquid and bacteria.
Preferably, the bacteria comprise one or a mixture of bacillus subtilis, bacillus silicate and bacillus laterosporus.
Preferably, the weight parts of the bacillus subtilis, the silicate bacillus and the bacillus laterosporus are 1:1: 1.
Preferably, the base solution comprises 0.3 part of magnesium sulfate, 4 parts of ammonium sulfate, 2 parts of sodium citrate, 9 parts of monopotassium phosphate, 3 parts of dipotassium phosphate, 20 parts of peptone, 3 parts of sodium chloride and 2 parts of monopotassium phosphate, and the pH value is 7.
The invention also aims to provide a preparation method of the microbial repairing agent for saline-alkali soil.
The invention aims to realize the purpose through the following technical scheme, and the preparation method of the saline-alkali soil microbial repairing agent at least comprises the following steps:
premixing: taking sodium alginate, ethylene-vinyl acetate copolymer, vinyl alcohol-ethylene copolymer and calcium chloride, putting into water, uniformly mixing, and after the reaction is finished, adjusting the pH value of the reaction system to be neutral;
and (3) synthesis of a repairing agent: and (3) adding the bacterial liquid and the polyallyl alcohol into the repairing agent to synthesize a mixture, uniformly mixing for 30-50min, and discharging to prepare the saline-alkali soil microbial repairing agent.
Preferably, in the premixing step, the reaction temperature is 180-190 ℃, and the reaction time is 40-60 min.
Preferably, in the premixing step, the pH value of the reaction system is 7-7.5.
The invention also aims to provide a using method of the microbial repairing agent for saline-alkali soil.
The purpose of the invention is realized by the following technical scheme, and the application method of the saline-alkali soil microbial repairing agent comprises the following steps:
land leveling: spreading crushed crop straws 500-;
deep ploughing soil: deeply ploughing 20-30cm of soil to uniformly mix crop straws, organic fertilizer and the saline-alkali soil microbial repairing agent; fully irrigating: supplementing water to the soil to ensure that the water content of the soil reaches 100 percent;
naturally stacking and repairing soil: naturally reacting and repairing the soil for 2-10 weeks, and spraying supplementary water at irregular time to keep the water content of the soil above 30%; after 1-3 weeks, or when the soil temperature exceeds 35-40 ℃, carrying out rotary tillage on the soil to ensure better soil remediation effect, wherein the whole balancing process can carry out rotary tillage on the soil for 1-2 times;
planting: and planting the soil after the remediation is finished.
In conclusion, the invention has the following beneficial effects:
1. the microbial repairing agent for saline-alkali soil prepared by the invention utilizes special microbes to act on the saline-alkali soil through mineralization, co-metabolism, biological concentration or accumulation and the like, so that the metabolic activity of the microbes is enhanced, and the effect of repairing the saline-alkali soil is improved.
2. After the soil is repaired by the saline-alkali soil microbial repairing agent, the pH value of the soil can be reduced by 0.1-0.3, the cation exchange capacity of the soil can be reduced by 10-30%, the medium and light saline-alkali soil with the salt content of less than 0.5% can be used for normally planting crops, the medium and heavy saline-alkali soil with the salt content of 0.5-1.2% can be used for planting saline-alkali tolerant plants, such as cotton, oil sunflower, medlar and other crops, and the germination rate can reach more than 90%.
Detailed Description
All materials referred to in the examples of the present invention are commercially available.
B, bacillus subtilis: china agricultural microbial strain preservation management center, Bacillus subtilis with the number accc 11025; silicate bacillus: china agricultural microbial strain preservation management center, silicate bacillus with the number accc 10013; b, B.laterosporus: china agricultural microbial culture collection management center, Bacillus laterosporus with the number accc 10390.
First, the manufacturing embodiment
Example 1
Premixing: taking 15kg of sodium alginate, 7kg of ethylene-vinyl acetate copolymer, 14kg of vinyl alcohol-ethylene copolymer and 4.5kg of calcium chloride, putting into 50kg of water, uniformly mixing, and after the reaction is finished, adjusting the pH value of a reaction system to 7, wherein the reaction temperature is 185 ℃, the reaction time is 50min, the average molecular weight of the ethylene-vinyl acetate copolymer is 2000, and the average molecular weight of the vinyl alcohol-ethylene copolymer is 2500;
preparing bacterial liquid: respectively putting 7kg of bacillus subtilis powder, 7kg of silicate bacillus powder and 7kg of bacillus laterosporus powder into 25kg of substrate liquid for culture, fermenting after rejuvenation, primary expansion and secondary expansion, and ventilating by an air compressor in the fermentation process to obtain bacterial liquid; wherein the base solution comprises 0.3 part of magnesium sulfate, 4 parts of ammonium sulfate, 2 parts of sodium citrate, 9 parts of monopotassium phosphate, 3 parts of dipotassium phosphate, 20 parts of peptone, 3 parts of sodium chloride and 2 parts of monopotassium phosphate, and the pH value is 7;
and (3) synthesis of a repairing agent: adding 40kg of bacterial liquid and 7kg of polypropylene alcohol into the mixture obtained by premixing, uniformly mixing for 40min, and discharging to prepare the saline-alkali soil microorganism repairing agent.
Example 2
Premixing: taking 11kg of sodium alginate, 5kg of ethylene-vinyl acetate copolymer, 12kg of vinyl alcohol-ethylene copolymer and 7kg of calcium chloride, putting into 50kg of water, uniformly mixing, and after the reaction is finished, adjusting the pH value of a reaction system to 7.5, wherein the reaction temperature is 190 ℃, the reaction time is 40min, the average molecular weight of the ethylene-vinyl acetate copolymer is 2000, and the average molecular weight of the vinyl alcohol-ethylene copolymer is 3000;
preparing bacterial liquid: respectively putting 5kg of bacillus subtilis powder, 5kg of silicate bacillus powder and 9kg of bacillus laterosporus powder into 20kg of substrate liquid for culture, fermenting after rejuvenation, primary expansion and secondary expansion, and ventilating by an air compressor in the fermentation process to obtain bacterial liquid; wherein the base solution comprises 0.3 part of magnesium sulfate, 4 parts of ammonium sulfate, 2 parts of sodium citrate, 9 parts of monopotassium phosphate, 3 parts of dipotassium phosphate, 20 parts of peptone, 3 parts of sodium chloride and 2 parts of monopotassium phosphate, and the pH value is 7;
and (3) synthesis of a repairing agent: adding 45kg of bacterial liquid and 9kg of polypropylene alcohol into the mixture obtained by premixing, uniformly mixing for 30min, and discharging to prepare the saline-alkali soil microbial repairing agent.
Example 3
Premixing: taking 19kg of sodium alginate, 9kg of ethylene-vinyl acetate copolymer, 12kg of vinyl alcohol-ethylene copolymer and 7kg of calcium chloride, putting into 50kg of water, uniformly mixing, and after the reaction is finished, adjusting the pH value of a reaction system to 7, wherein the reaction temperature is 180 ℃, the reaction time is 60min, the average molecular weight of the ethylene-vinyl acetate copolymer is 2500, and the average molecular weight of the vinyl alcohol-ethylene copolymer is 3000;
preparing bacterial liquid: respectively putting 5kg of bacillus subtilis powder, 9kg of silicate bacillus powder and 5kg of bacillus laterosporus powder into 20kg of substrate liquid for culture, fermenting after rejuvenation, primary expansion and secondary expansion, and ventilating by an air compressor in the fermentation process to obtain bacterial liquid; wherein the base solution comprises 0.3 part of magnesium sulfate, 4 parts of ammonium sulfate, 2 parts of sodium citrate, 9 parts of monopotassium phosphate, 3 parts of dipotassium phosphate, 20 parts of peptone, 3 parts of sodium chloride and 2 parts of monopotassium phosphate, and the pH value is 7;
and (3) synthesis of a repairing agent: adding 45kg of bacterial liquid and 5kg of polypropylene alcohol into the mixture obtained by premixing, uniformly mixing for 50min, and discharging to prepare the saline-alkali soil microbial repairing agent.
Example 4
Premixing: taking 11kg of sodium alginate, 5kg of ethylene-vinyl acetate copolymer, 16kg of vinyl alcohol-ethylene copolymer and 2kg of calcium chloride, putting into 50kg of water, uniformly mixing, and after the reaction is finished, adjusting the pH value of a reaction system to 7.5, wherein the reaction temperature is 190 ℃, the reaction time is 50min, the average molecular weight of the ethylene-vinyl acetate copolymer is 1500, and the average molecular weight of the vinyl alcohol-ethylene copolymer is 2500;
preparing bacterial liquid: respectively putting 9kg of bacillus subtilis powder, 5kg of silicate bacillus powder and 5kg of bacillus laterosporus powder into 30kg of substrate liquid for culture, fermenting after rejuvenation, primary expansion and secondary expansion, and ventilating by an air compressor in the fermentation process to obtain bacterial liquid; wherein the base solution comprises 0.3 part of magnesium sulfate, 4 parts of ammonium sulfate, 2 parts of sodium citrate, 9 parts of monopotassium phosphate, 3 parts of dipotassium phosphate, 20 parts of peptone, 3 parts of sodium chloride and 2 parts of monopotassium phosphate, and the pH value is 7;
and (3) synthesis of a repairing agent: adding 35kg of bacterial liquid and 9kg of polypropylene alcohol into the mixture obtained by premixing, uniformly mixing for 40min, and discharging to prepare the saline-alkali soil microorganism repairing agent.
Second, preparation of comparative example
Comparative example 1
Preparing bacterial liquid: respectively putting 7kg of bacillus subtilis powder, 7kg of silicate bacillus powder and 7kg of bacillus laterosporus powder into 25kg of substrate liquid for culture, fermenting after rejuvenation, primary expansion and secondary expansion, and ventilating by an air compressor in the fermentation process to obtain bacterial liquid; wherein the base solution comprises 0.3 part of magnesium sulfate, 4 parts of ammonium sulfate, 2 parts of sodium citrate, 9 parts of monopotassium phosphate, 3 parts of dipotassium phosphate, 20 parts of peptone, 3 parts of sodium chloride and 2 parts of monopotassium phosphate, and the pH value is 7; discharging, and preparing the saline-alkali soil microbial repairing agent.
Comparative example 2
Premixing: taking 15kg of sodium alginate, 7kg of ethylene-vinyl acetate copolymer, 14kg of vinyl alcohol-ethylene copolymer and 4.5kg of calcium chloride, putting into 50kg of water, uniformly mixing, and after the reaction is finished, adjusting the pH value of a reaction system to 7, wherein the reaction temperature is 185 ℃, the reaction time is 50min, the average molecular weight of the ethylene-vinyl acetate copolymer is 2000, and the average molecular weight of the vinyl alcohol-ethylene copolymer is 2500;
preparing bacterial liquid: respectively putting 7kg of bacillus subtilis powder, 7kg of silicate bacillus powder and 7kg of bacillus laterosporus powder into 25kg of substrate liquid for culture, fermenting after rejuvenation, primary expansion and secondary expansion, and ventilating by an air compressor in the fermentation process to obtain bacterial liquid; wherein the base solution comprises 0.3 part of magnesium sulfate, 4 parts of ammonium sulfate, 2 parts of sodium citrate, 9 parts of monopotassium phosphate, 3 parts of dipotassium phosphate, 20 parts of peptone, 3 parts of sodium chloride and 2 parts of monopotassium phosphate, and the pH value is 7;
and (3) synthesis of a repairing agent: adding 40kg of bacterial liquid into the mixture obtained by premixing, uniformly mixing for 40min, and discharging to prepare the saline-alkali soil microbial repairing agent.
Third, application example
Example 5
Land leveling: the initial salt content of the soil is 0.5%, 750 kg/mu of crushed crop straws are paved on the surface of the saline-alkali soil, then 1500 kg/mu of organic fertilizer is paved, and then the saline-alkali soil microbial repairing agent prepared in the embodiment 1 is uniformly paved on the surface, wherein the quantity is 60 kg/mu;
deep ploughing soil: deeply ploughing soil for 25cm to uniformly mix crop straws, organic fertilizer and the saline-alkali soil microbial repairing agent;
fully irrigating: supplementing water to the soil to ensure that the water content of the soil reaches 100 percent;
naturally stacking and repairing soil: naturally reacting and repairing the soil for 6 weeks, and spraying supplementary water at variable time to keep the water content of the soil above 30%; after 3 weeks, the soil is subjected to rotary tillage to ensure a better soil remediation effect, and the soil can be subjected to rotary tillage for 2 times in the whole balancing process;
planting: and planting the potatoes in the soil after the remediation is finished.
Example 6
Land leveling: the initial salt content of the soil is 0.5%, 500 kg/mu of crushed crop straws are paved on the surface of the saline-alkali soil, then 1000 kg/mu of organic fertilizer is paved, and then the saline-alkali soil microbial repairing agent prepared in the embodiment 2 is uniformly paved on the surface, wherein the quantity is 20 kg/mu;
deep ploughing soil: deeply ploughing soil for 30cm to uniformly mix crop straws, organic fertilizer and the saline-alkali soil microbial repairing agent;
fully irrigating: supplementing water to the soil to ensure that the water content of the soil reaches 100 percent;
naturally stacking and repairing soil: naturally reacting and repairing the soil for 10 weeks, and spraying supplementary water at irregular time to keep the water content of the soil above 30%; after 1 week, carrying out rotary tillage on the soil to ensure a better soil remediation effect, wherein the whole balancing process can carry out rotary tillage on the soil for 2 times;
planting: and planting the potatoes in the soil after the remediation is finished.
Example 7
Land leveling: the initial salt content of the soil is 0.5%, 1000 kg/mu of crushed crop straws are paved on the surface of the saline-alkali soil, 2000 kg/mu of organic fertilizer is paved, and then the microbial repairing agent for the saline-alkali soil prepared in the embodiment 3 is uniformly paved on the surface of the saline-alkali soil, wherein the quantity of the microbial repairing agent is 20 kg/mu;
deep ploughing soil: deeply ploughing soil for 30cm to uniformly mix crop straws, organic fertilizer and the saline-alkali soil microbial repairing agent;
fully irrigating: supplementing water to the soil to ensure that the water content of the soil reaches 100 percent;
naturally stacking and repairing soil: naturally reacting and repairing the soil for 2 weeks, and spraying supplementary water at variable time to keep the water content of the soil above 30%; when the soil temperature reaches 35 ℃, the soil is subjected to rotary tillage to ensure a better soil remediation effect, and the soil can be subjected to rotary tillage for 2 times in the whole balancing process;
planting: and planting the potatoes in the soil after the remediation is finished.
Example 8
Land leveling: the initial salt content of the soil is 0.5%, 500 kg/mu of crushed crop straws are spread on the surface of the saline-alkali soil, then 1000 kg/mu of organic fertilizer is spread, and then the saline-alkali soil microbial repairing agent prepared in the embodiment 4 is uniformly spread on the surface, wherein the quantity is 100 kg/mu;
deep ploughing soil: deeply ploughing 20cm of soil to uniformly mix crop straws, organic fertilizer and the saline-alkali soil microbial repairing agent;
fully irrigating: supplementing water to the soil to ensure that the water content of the soil reaches 100 percent;
naturally stacking and repairing soil: naturally reacting and repairing the soil for 10 weeks, and spraying supplementary water at irregular time to keep the water content of the soil above 30%; when the soil temperature reaches 40 ℃, the soil is subjected to rotary tillage to ensure a better soil remediation effect, and the soil can be subjected to rotary tillage for 1 time in the whole balancing process;
planting: and planting the potatoes in the soil after the remediation is finished.
Fourth, application comparative example
Comparative example 3:
repairing agent: the repair agent prepared in comparative example 1;
application environment: the same environment as the application environment of the repairing agent prepared in example 1 was used.
Comparative example 4:
repairing agent: the repair agent prepared in comparative example 2;
application environment: the same environment as the application environment of the repairing agent prepared in example 1 was used.
Comparative example 5: blank control.
And fifthly, testing the performances of the activators prepared in the above examples and comparative examples.
And taking the soil before being repaired by applying the repairing agent and the soil after being repaired by applying the repairing agent, and testing the pH value and the cation exchange capacity reduction rate of the soil. Meanwhile, the germination and yield of the potatoes were determined.
The evaluation index results are shown in table 1.
Table 1 results of performance test of each example and comparative preparation of microbial remediation agent for saline and alkaline land
Results of Performance testing | pH of soil | Decrease ratio of cation exchange amount (%) | Potato Germination (%) | Potato yield (kilogram/mu) |
Example 5 | 7.9 | 30 | 95 | 3592 |
Example 6 | 7.9 | 27 | 92 | 3510 |
Example 7 | 8.0 | 21 | 94 | 3423 |
Example 8 | 8.1 | 23 | 93 | 3319 |
Comparative example 3 | 8.2 | 10 | 82 | 1980 |
Comparative example 4 | 8.2 | 18 | 86 | 2218 |
Comparative example 5 | 8.2 | — | 50 | 1512 |
As can be seen from the table, the microbial repairing agent for saline-alkali soil prepared by the invention acts on saline-alkali soil through mineralization, co-metabolism, biological concentration or accumulation and the like, so that the metabolic activity of microorganisms is enhanced, and the effect of repairing saline-alkali soil is improved. After the saline-alkali soil is repaired, the pH value of the soil can be reduced by 0.1-0.3, the cation exchange capacity of the soil can be reduced by 10-30%, the medium and light saline-alkali soil with the salt content of less than 0.5% can be used for normally planting crops, the germination rate can reach more than 90%, and the yield can reach the normal level of the crops.
Wherein, the repairing agent used in the comparative example 3 has no premixing step in the preparation process, and in the process of utilizing and repairing saline-alkali soil, the repairing agent does not provide a proper growth environment for microorganisms, so that the metabolic activity of the microorganisms is reduced, and the capability of the microorganisms for repairing the saline-alkali soil is weakened, so that the germination rate and the yield of the potato yield are lower than those of the repairing agent used in the example 5.
In the preparation process of the repairing agent used in the comparative example 4, no polypropylene glycol is added, the bacterial liquid is only adsorbed on the generated cross-linked polymer, the microorganisms in the bacterial liquid are easy to fall off, the metabolic activity of the microorganisms is reduced after the microorganisms fall off, and the capability of the microorganisms for repairing the saline-alkali soil is weakened, so that the germination rate and the yield of the potato yield are lower than those of the repairing agent used in the example 5.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (7)
1. A saline-alkali soil microbial repairing agent is characterized by comprising, by weight, 5-9 parts of ethylene-vinyl acetate copolymer, 12-16 parts of vinyl alcohol-ethylene copolymer, 11-19 parts of sodium alginate, 2-7 parts of calcium chloride, 35-45 parts of bacterial liquid and 5-9 parts of polypropylene glycol; the bacterial liquid is a mixture of a substrate liquid and bacteria; the bacteria comprise one or a mixture of more of bacillus subtilis, bacillus silicate and bacillus laterosporus; the preparation method of the saline-alkali soil microbial repairing agent at least comprises the following steps: premixing: taking sodium alginate, ethylene-vinyl acetate copolymer, vinyl alcohol-ethylene copolymer and calcium chloride, putting into water, uniformly mixing, and after the reaction is finished, adjusting the pH value of the reaction system to be neutral; and (3) synthesis of a repairing agent: and (3) adding the bacterial liquid and the polyallyl alcohol into the repairing agent to synthesize a mixture, uniformly mixing for 30-50min, and discharging to prepare the saline-alkali soil microbial repairing agent.
2. The microbial repairing agent for saline-alkali soil as claimed in claim 1, wherein the average molecular weight of the ethylene-vinyl acetate copolymer is 1500-2500, and the average molecular weight of the vinyl alcohol-ethylene copolymer is 2000-3000.
3. The microbial repairing agent for saline-alkali soil according to claim 1, wherein the bacillus subtilis, the bacillus silicate and the bacillus laterosporus are prepared from the following components in parts by weight of 1:1: 1.
4. the microbial repairing agent for saline-alkali soil according to claim 1, wherein the base solution comprises 0.3 part of magnesium sulfate, 4 parts of ammonium sulfate, 2 parts of sodium citrate, 9 parts of monopotassium phosphate, 3 parts of dipotassium hydrogen phosphate, 20 parts of peptone, 3 parts of sodium chloride, 2 parts of monopotassium phosphate and pH 7.
5. The microbial repairing agent for saline-alkali soil as claimed in claim 1, wherein in the premixing step, the reaction temperature is 180-190 ℃ and the reaction time is 40-60 min.
6. The microbial repairing agent for saline-alkali soil according to claim 1, wherein in the premixing step, the pH value of a reaction system is 7-7.5.
7. A method for using the microbial repairing agent for the saline-alkali soil as described in any one of claims 1 to 4, wherein the method comprises the following steps:
land leveling: spreading crushed crop straws 500-;
deep ploughing soil: deeply ploughing 20-30cm of soil to uniformly mix crop straws, organic fertilizer and the saline-alkali soil microbial repairing agent; fully irrigating: supplementing water to the soil to ensure that the water content of the soil reaches 100 percent;
naturally stacking and repairing soil: naturally reacting and repairing the soil for 2-10 weeks, and spraying supplementary water at irregular time to keep the water content of the soil above 30%; after 1-3 weeks, or when the soil temperature exceeds 35-40 ℃, carrying out rotary tillage on the soil to ensure better soil remediation effect, wherein the whole balancing process can carry out rotary tillage on the soil for 1-2 times;
planting: and planting the soil after the remediation is finished.
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