CN108927406A - A kind of arsenic in soil pollution ecology restorative procedure and application - Google Patents
A kind of arsenic in soil pollution ecology restorative procedure and application Download PDFInfo
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- CN108927406A CN108927406A CN201810720905.4A CN201810720905A CN108927406A CN 108927406 A CN108927406 A CN 108927406A CN 201810720905 A CN201810720905 A CN 201810720905A CN 108927406 A CN108927406 A CN 108927406A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
Abstract
A kind of arsenic in soil pollution ecology restorative procedure and application, the technology belong to ecological environmental protection field.The technology is realized by following steps: starch being added in the bioreactor, soya-bean cake, calcium carbonate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ferrous sulfate etc., add enough water, stirring, it mixes, heat sterilization, it is cooling, Penicillium notatum etc. is inoculated with beneficial to bacterial strain, fermentation, it is cooling, mixture, it is canned, in being manured into soil, naturally culture 6-18 months, it can continue to convert escaping gas for the arsenic in soil, it is evaporate into air from soil, to remove the arsenic in soil, eliminate soil and groundwater arsenic pollution, soil protection environment, soil protection production and ecological functions, protect groundwater environment, it ensures food safety.
Description
Technical field
The invention belongs to ecological environmental protection field, in particular to a kind of arsenic in soil pollution ecology restorative procedure and application.
Background technique
Arsenic is a kind of environmental contaminants that toxicity is very strong.Arsenic in natural soils is mainly derived from soil parent material.By
During rock weathering forms soil, the content (relative to soil parent material) of arsenic has apparent enrichment.Mankind's activity such as mining,
It smelts, dirty filling, apply fertilizer, the application of insecticide and timber preservative all may cause soil arsenic pollution.Excessive Arsenic is accumulated in soil,
It will cause crop failure and quality decline, while may cause water source and atmosphere pollution, and the way such as food chain and water body can be passed through
Diameter enters human body.In recent years studies have shown that the arsenic of low amounts can also seriously endanger human health.
2016, it was one that " Soil Pollution Control action plan ", " Soil Pollution Control action plan ", which is formally issued and implemented,
The item huge soil pollution recovery project of scale of investment, also will be a long-term engineering.Due to soil remediation Its Relevant Technology Standards
The many difficulties implemented with soil remediations such as the shortages of basic data, interior in recent years, China's Soil Pollution Control also needs first to lead to
It crosses pilot demonstration and part is carried out, then realize the process of scale development.Basic data and the accumulation and reparation for repairing experience
The deposit of technology by be China's current stage soil remediation vital task.
China is arsenic minerals big country, arsenic minerals be widely distributed in the Central-South and southwestern Hunan in China, Yunnan, Guangxi, Guangdong etc. save,
Area.Arsenic minerals be easy to cause the pollution of neighbouring soil and water body in exploitation or smelting process.According to Wang Zhengang etc. to Shimen Realgar Mine
3 neighbouring villages investigate discovery, and soil arsenic content is 84~296mg/kg, this shows soil by different degrees of pollution.Arsenic
Water body migration stain large area agricultural land soil can also be passed through.Because paddy field mostly uses irrigation canals and ditches to irrigate, near mining and smeltery
Water paddy soil contamination phenomenon is on the rise, if there is large area in Hunan Changning county by arsenic polluted water rice field, arsenic content is 92~
840mg/kg；There are more than 8000 hectares of paddy fields by arsenic pollution in Hubei.Cause in Bangladesh since underground water is excessively high containing arsenic
70000000 people's drinking water arsenic contents are exceeded；With this irrigation water of well water containing arsenic rice field, allow for 0 in the time less than 10 years~
15cm soil horizon arsenic content is increased to 83mg/kg, and local arsenic in soil background value is only 4~8mg/kg.
The biological effect and its toxicity of arsenic, mainly due to sulfydryl (- SH), cystine and the half Guang ammonia in arsenic and zymoprotein
The amino (- N H) of sour sulfur-bearing has very strong affinity, so that histocyte breathing is obstructed in conjunction with after, mitochondrial swelling, to make generation
Thank stopping, cell death.For one kind, " the known mankind are carcinogenic by the World Health Organization (WHO) and U.S.EPA (EPA) deciding grade and level for arsenic
Substance ", human body exposes arsenic for a long time can lead to the canceration of the internal organs such as cutaneum carcinoma and kidney, liver, bladder.The approach of arsenic poisoning is main
Enter digestive system by drinking-water and food, the exhaust gas containing arsenic of industrial discharge enters the respiratory system.As3+It can be with the mercapto of human body enzyme
Base junction is closed, inhibitory enzyme activity, leads to carbohydrate metabolism disturbance, the content of acetone in blood and tissue increases and nerve endings function
Disorder.As5+Toxic effect is slower, it can destroy mitochondria oxidative phosphorylation, phosphate can be replaced to generate unstable
Arsenate, it may appear that the sequelae such as polyneuritis, myelitis, hypoplastic anemia.The toxic dose of adult arsenious acid
For 5~50mg/kg, lethal dose is 100~300mg/kg；The half lethal concentration (LD50) of arsenious acid is 10mg/kg, and arsenic acid
LD50 be 138mg/kg.
With the increase of arsenic amount, soil bacteria and actinomyces sum are substantially reduced, and arsenic is to Soil hydrolase system (urea
Enzyme, phosphatase and protease) and oxidoreducing enzyme (dehydrogenase, polyphenol oxidase and catalase) have different degrees of suppression
System.Arsenic inhibits CO in Soil Microorganism activity2Generation, significantly reduce the activity of denitrifying bacteria.Arsenic is to soil fixed nitrogen
Bacterium, phosphate-solubilizing bacteria and cellulose-decomposing bacteria have inhibiting effect, and wherein trichoderma and Bacterium anthracoides are most sensitive to arsenic, and Soybean Root
Tumor bacterium and the rigid spirillum patience highest containing rouge.
Arsenic pollution and arsenic species are a global problems.Japanese government is by arsenic poisoning and lead poisoning, minamata disease, bone
Seriously pollution disease is mutually arranged side by side for pain disease etc., is declared as fourth environmental disease.The toxicity of general arsenic is successively are as follows: and arsenic hydride > trivalent inorganic arsenic >
Pentavalent inorganic arsenic > organo-arsenic > element arsenic.Arsenic is plant non-essential element, in general plant As content mobility scale be 0.01~
5mg/kg.Plant can absorb arsenic from external environment in growth and development process, and the arsenic of low amounts can stimulate the growth of plant.But
Higher arsenic content can generate toxic effect to plant in soil, endanger its growth and development, make crop yield and quality decline.
The soil that property is different, purposes is different, pollution level is different, the technology and methods repaired are not also identical.It is common
Arsenic in soil pollution control recovery technique has following several: immobilization, stabilization technology, passivating technique, Soil leaching technology, original position
Electric repairing technique, gram local method, bioremediation technology etc..Fixation, Dunhua, stabilization technology are at low cost, but materials curing agent
It is a large amount of using the structure that can destroy soil, therefore, which is not suitable for the soil remediation of large area.Soil leaching technology is used
Leacheate elution, the method easily cause the leaching loss and precipitating of certain nutrients, and therefore, this method is suitable for the small weight of area
The soil remediation of metallic pollution.Ma et al. has found a kind of plant-Pteris vittata L in the middle part of Fla., can super enrichment
Arsenic is planted Pteris vittata L in laboratory, is cultivated 6 weeks, the content of arsenic has reached 22630mg/kg in accessory pinna.Chen Tongbin etc., Wei Chao
Sun etc. have also discovered the super enriching plant-Pteris vittata L and cretan brake of arsenic in Hunan China.Recently it was also discovered that many plants
Object can be good at being enriched with arsenic, such as: Creeping bentgrass, Meng Tana chrysanthemum, knotweed vehicle, Bermuda grass etc..But these plants are mostly
Shade plant among long term growth virgin soil out of office open country, is cultivated for large area agricultural land soil arsenic, is exposed to the sun intolerant to sunburst and pH
The great change of value, often undergrowth, repairing effect is bad, simultaneously because super build-up properties are substantially after continuous multi-generation vegetative propagation
It degenerates, loses repair function.
This technology uses multiple-microorganism mix bacterium agent rehabilitating soil arsenic, in conjunction with soil moisture and oxidation-reduction potential tune
The compound of arsenic in soil and arsenic is restored and/or is oxidized to methyl arsenic, dimethyl arsenic, arsenic trimethide and Shenization Qing Den gas by control,
It is evaporated from soil, so that soil arsenic pollution, soil protection and groundwater environment are removed, the compound height produced using this law
It is high to imitate microbial inoculum treatment effeciency, at low cost, environment friendly is high, and it is easy to operate, without secondary pollution, large-scale industry easy to accomplish
Change application.
Summary of the invention
The technical issues of solution: the present invention is directed to soil arsenic pollution, especially agricultural land soil and underground water, Metallurgical Factory, gold
Belong to stope and chemical plant contaminated sites, invents a kind of high efficiency composition microbial inoculum, it is within a short period of time that soil arsenic pollution is most of
The gaseous volatilizations such as methyl arsenic are aoxidized and/or are reduced into, to remove harm of the soil As to environment and the mankind.
Technical solution: a kind of arsenic in soil pollution ecology restorative procedure and application, step are that a kind of arsenic in soil pollution ecology is repaired
Compound method and application, it is characterised in that step are as follows: a. is added 100-900 grams of starch in 10 liters of bioreactors, rice and/
Or 500-2000 grams of wheat stalk powder, 60-300 grams of beancake powder, 40-180 grams of dipotassium hydrogen phosphate, 30-160 grams of biphosphate
Potassium, 10-120 grams of calcium carbonate powder, 5-60 grams of ferrous sulfate, 10-50 grams of magnesium sulfate, 7000 milliliters of water start blender stirring
Mixed solution starts heating steam pipe, heats mixed solution, and lower 126 DEG C of 0.1Mpa are kept for 45 minutes, and sterilizing terminates, and is cooled to
The Penicillium notatum of 30 DEG C of inoculations 0.5-7% (mass/volume), keeps stirring, 50-120 revs/min of agitator speed, ventilatory capacity
0.5-1 goes up to the air gas/liter liquid (volume/volume/minute), and constant temperature is mixed 72-120 hours, and fermentation ends put tank.Equally
Method, be inoculated with the aspergillus niger of 0.5-7% (mass/volume), sickle-like bacteria respectively in above-mentioned same liquid fermentation medium, start
Blender, 50~120 revs/min of agitator speed, ventilatory capacity 0.5-1 goes up to the air gas/liter liquid (volume/volume/minute), constant temperature
Mixed culture 72-120 hours, fermentation ends put tank.B. by above-mentioned a, treated that 3 kinds of different fermentation liquids are concentrated, and filters, protects
Reserved filtrate keeps viable bacteria and spore content >=0.5 hundred million/milliliter in filtrate, 3 kinds of bacterium solutions is mixed into composite bacteria liquid system in equal volume
Agent.C. by above-mentioned b, treated that composite bacteria agent sprays agricultural land soil surface, uniformly sprays 50-200 kilograms of composite bacteria agent per acre,
It is primary to plough soil, pours water immediately so that soil surface has ponding 10cm or more, and keep soil surface dampness 20 days with
On, 50-200 kilograms of composite bacteria agent is sprayed for the second time, and pour water immediately, so that agricultural land soil surface layer ponding 10cm or more, maintains
Field dampness 20 days or more, according to arsenic in soil difference pollution level, repeat spraying microbial inoculum number 4-10 times, bacterium was sprayed every time
It will pour water immediately after agent, and keep soil surface ponding 10cm or more, and holding 20 days or more dampnesses, in favor of microbial inoculum
Middle microbial nutrition body and gemma are proliferated in the soil, biological oxidation and/or reduction arsenic become methyl arsenic, dimethyl arsenic, front three
The gases such as base arsenic and arsenic hydride.
A kind of composite bacteria agent of any rehabilitating soil arsenic pollution and its farmland, refuse landfill, municipal sludge plus
Petrol station, paper mill, stope, electroplate factory, nearby soil pollution arsenic contaminates for electrolysis plant, metallurgical works, contaminated sites and farm
Application in reparation.3 kinds of microorganisms can be happy from Chinese Academy of Sciences bacterium guarantor center, the Chinese Academy of Agricultural Sciences bacterium guarantor center, Nanjing used in this law
Ecological environment Science and Technology Ltd. can be helped to buy.
The utility model has the advantages that the present invention can eliminate arsenic pollution object in soil and groundwater to greatest extent, eliminate to greatest extent
Soil arsenic pollution and its destruction to environment and the harm to the mankind, are conducive to Ecological Sustainable Development.
The present invention is compared with prior art:
1) the soil arsenic pollution composite bacteria agent researched and developed in the present invention, production method simplicity, technically simple easy, operability
By force, low in cost, practical；
2) present invention removes soil arsenic pollution using microbiological oxidation reduction technique, can be really soil and/or underground
Arsenic is converted into gaseous volatilization and goes out in water, reduces the arsenic polluted in soil to the maximum extent, fundamentally eliminates in soil
Arsenic is compared with currently used single solidification hair/deactivation method, is real removal soil arsenic pollution, residue and secondary dirt
It is seldom to contaminate object, selected 3 kinds of compound bacterias be all it is naturally occurring in soil natural circle, do not pollute with other harm, be a kind of
Eco-friendly arsenic pollution administering method is the current best approach.
Detailed description of the invention
Fig. 1 is using this technology processing arsenic in soil pollution course comparison.
Specific embodiment
Below in conjunction with example, the invention will be further described:
Embodiment 1:
1. 100 grams of starch is added in 10 liters of bioreactors, 50 grams of rice straw powder, 60 grams of beancake powders, 40 grams
Dipotassium hydrogen phosphate, 30 grams of potassium dihydrogen phosphates, 10 grams of calcium carbonate powders, 5 grams of ferrous sulfate, 10 grams of magnesium sulfate, 7000 milliliters of water are opened
Dynamic blender is stirred solution, starts heating steam pipe, heats mixed solution, and lower 126 DEG C of 0.1Mpa are kept for 45 minutes, sterilizing
Terminate, is cooled to the Penicillium notatum of 30 DEG C of 0.5% (mass/volumes) of inoculation, keeps stirring, 50 revs/min of agitator speed, ventilate
0.5 lift-off gas/liter liquid (volume/volume/minute) of amount, constant temperature are mixed 72 hours, and fermentation ends put tank.Same side
Method is inoculated with aspergillus niger, the sickle-like bacteria of 0.5% (mass/volume) in above-mentioned same liquid fermentation medium respectively, starts stirring
Device, 50 revs/min of agitator speed, ventilatory capacity 0.5 goes up to the air gas/liter liquid (volume/volume/minute), constant temperature mixed culture 72
Hour, fermentation ends put tank.
2. by above-mentioned 1, treated that 3 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, keeps viable bacteria and bud in filtrate
3 kinds of bacterium solutions are mixed into composite bacteria liquid preparation by spore content >=0.5 hundred million/milliliter in equal volume.
3. by above-mentioned 2, treated that composite bacteria agent sprays agricultural land soil surface, before application, ploughs soil, per acre uniformly spray
200 kilograms of composite bacteria agent are applied, it is primary to plough soil, pours water immediately, so that soil surface has ponding 10cm or more, and keeps soil
Surface moisture state 20 days or more, 200 kilograms of composite bacteria agents are sprayed for the second time, and pour water immediately, so that agricultural land soil surface layer product
Water 10cm or more, maintain field dampness 20 days or more, according to arsenic in soil difference pollution level, repeat spraying microbial inoculum number 10
It is secondary, it will pour water immediately after spraying microbial inoculum every time, and keep soil surface ponding 10cm or more, and holding 20 days or more moist shapes
State, in favor of microbial nutrition body in microbial inoculum and gemma are proliferated in the soil, biological oxidation and/or reduction arsenic become methyl arsenic,
The gases such as dimethyl arsenic, arsenic trimethide and arsenic hydride.
Embodiment 2:
1. 500 grams of starch is added in 10 liters of bioreactors, 1000 grams of wheat stalk powder, 100 grams of beancake powders, 80
Gram 60 grams of potassium dihydrogen phosphates of dipotassium hydrogen phosphate, 30 grams of calcium carbonate powders, 20 grams of ferrous sulfate, 30 grams of magnesium sulfate, 7000 milliliters of water,
It starts blender and is stirred solution, start heating steam pipe, heat mixed solution, lower 126 DEG C of 0.1Mpa are kept for 45 minutes, are gone out
Bacterium terminates, and is cooled to the Penicillium notatum of 30 DEG C of 3% (mass/volumes) of inoculation, keeps stirring, 50-120 revs/min of agitator speed,
Ventilatory capacity 0.8 goes up to the air gas/liter liquid (volume/volume/minute), and constant temperature is mixed 80 hours, and fermentation ends put tank.Equally
Method, be inoculated with aspergillus niger, the sickle-like bacteria of 3% (mass/volume) respectively in above-mentioned same liquid fermentation medium, start stirring
Device, 80 revs/min of agitator speed, ventilatory capacity 0.8 goes up to the air gas/liter liquid (volume/volume/minute), constant temperature mixed culture 80
Hour, fermentation ends put tank.
2. by above-mentioned 1, treated that 3 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, keeps viable bacteria and thatch in filtrate
3 kinds of bacterium solutions are mixed into composite bacteria liquid preparation by spore content >=0.5 hundred million/milliliter in equal volume.
3. by above-mentioned 2, treated that composite bacteria agent sprays agricultural land soil surface, before application, ploughs soil, per acre uniformly spray
Composite bacteria agent double centner is applied, it is primary to plough soil, pours water immediately, so that soil surface has ponding 10cm or more, and keeps soil
Surface moisture state 20 days or more, double centner composite bacteria agent is sprayed for the second time, and pour water immediately, so that agricultural land soil surface layer product
Water 10cm or more, maintain field dampness 20 days or more, according to arsenic in soil difference pollution level, repeat spraying microbial inoculum number 6
It is secondary, it will pour water immediately after spraying microbial inoculum every time, and keep soil surface ponding 10cm or more, and holding 20 days or more moist shapes
State, in favor of microbial nutrition body in microbial inoculum and gemma are proliferated in the soil, biological oxidation and/or reduction arsenic become methyl arsenic,
The gases such as dimethyl arsenic, arsenic trimethide and arsenic hydride.
Embodiment 3
1. 900 grams of starch is added in 10 liters of bioreactors, rice and/or 2000 grams of wheat stalk powder, 300 grams
Beancake powder, 180 grams of dipotassium hydrogen phosphates, 160 grams of potassium dihydrogen phosphates, 120 grams of calcium carbonate powders, 60 grams of ferrous sulfate, 50 grams of sulfuric acid
Magnesium, 7000 milliliters of water, starts blender and is stirred solution, starts heating steam pipe, heats mixed solution, 126 under 0.1Mpa
DEG C keep 45 minutes, sterilizing terminates, be cooled to 30 DEG C inoculation 7% (mass/volume) Penicillium notatums, keep stirring, blender turn
120 revs/min of speed, ventilatory capacity 1 go up to the air gas/liter liquid (volume/volume/minute), and constant temperature is mixed 96 hours, fermentation knot
Beam puts tank.Same method is inoculated with aspergillus niger, the sickle of 7% (mass/volume) in above-mentioned same liquid fermentation medium respectively
Knife bacterium starts blender, and 120 revs/min of agitator speed, ventilatory capacity 1 goes up to the air gas/liter liquid (volume/volume/minute), permanent
Temperature mixed culture 96 hours, fermentation ends put tank.
2. by above-mentioned 1, treated that 3 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, keeps viable bacteria and bud in filtrate
3 kinds of bacterium solutions are mixed into composite bacteria liquid preparation by spore content >=0.5 hundred million/milliliter in equal volume.
3. by above-mentioned 2, treated that composite bacteria agent sprays agricultural land soil surface, uniformly sprays 50 kilograms of composite bacteria agent per acre,
It is primary to plough soil, pours water immediately so that soil surface has ponding 10cm or more, and keep soil surface dampness 20 days with
On, 50 kilograms of composite bacteria agents are sprayed for the second time, and pour water immediately, so that agricultural land soil surface layer ponding 10cm or more, maintains field
Dampness 20 days or more, according to arsenic in soil difference pollution level, repeat spraying microbial inoculum number 4 times sprayed after microbial inoculum all every time
It to pour water immediately, and keep soil surface ponding 10cm or more, and holding 20 days or more dampnesses, in favor of life micro- in microbial inoculum
Object trophosome and gemma be proliferated in the soil, biological oxidation and/or reduction arsenic become methyl arsenic, dimethyl arsenic, arsenic trimethide and
The gases such as arsenic hydride.
Application example of the invention:
By the technology of the present invention be used to pollute various heavy soil remediation using.
1, it referring to embodiment 1, is handled using this technology and contains As contaminated soil, after continuous 4 times are handled 12 months,
As, which is remained, with the control soil of this technology not being used to compare, in soil reduces by 35% (Fig. 1)；
2, it referring to embodiment 2, is handled using this technology and contains As contaminated soil, handled 12 months by continuous 10 times
Afterwards, As, which is remained, with the control soil of this technology not being used to compare, in soil reduces 47%；
3, it referring to embodiment 3, is handled using this technology and contains As contaminated soil, handled 12 months by continuous 10 times
Afterwards, As, which is remained, with the control soil of this technology not being used to compare, in soil reduces 44%.
Claims (5)
1. a kind of arsenic in soil pollution ecology restorative procedure and application, it is characterised in that step are as follows:
A. 500-2000 grams of 100-900 grams of starch, rice and/or wheat stalk powder are added in 10 liters of bioreactors,
60-300 grams of beancake powder, 40-180 grams of dipotassium hydrogen phosphate, 30-160 grams of potassium dihydrogen phosphate, 10-120 grams of calcium carbonate powder, 5-60
Gram ferrous sulfate, 10-50 grams of magnesium sulfate, 7000 milliliters of water start blender and are stirred solution, start heating steam pipe, add
Hot mixing solution, lower 126 DEG C of 0.1Mpa are kept for 45 minutes, and sterilizing terminates, and is cooled to 30 DEG C of inoculation 0.5-7% (mass/volume)
Penicillium notatum, keep stirring, 50-120 revs/min of agitator speed, ventilatory capacity 0.5-1 go up to the air gas/liter liquid (volume/volume/
Minute), constant temperature is mixed 72-120 hours, and fermentation ends put tank.Same method, in above-mentioned same liquid fermented and cultured
Base is inoculated with the aspergillus niger of 0.5-7% (mass/volume), sickle-like bacteria respectively, starts blender, and 50-120 revs/min of agitator speed
Clock, ventilatory capacity 0.5-1 go up to the air gas/liter liquid (volume/volume/minute), and constant temperature is mixed 72-96 hours, and fermentation ends are put
Tank.
B. by above-mentioned a, treated that 3 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, and viable bacteria and gemma in filtrate is kept to contain
>=0.5 hundred million/milliliter is measured, 3 kinds of bacterium solutions are mixed into composite bacteria liquid preparation in equal volume.
C. by above-mentioned b, treated that composite bacteria agent sprays agricultural land soil surface, before administration, ploughs soil, uniformly sprays per acre
50-200 kilograms of composite bacteria agent, it is primary to plough soil, pours water immediately, so that soil surface has ponding 10cm or more, and keeps soil
Earth surface moisture state 20 days or more, 50-200 kilograms of composite bacteria agent is sprayed for the second time, and pour water immediately, so that agricultural land soil table
Lamination water 10cm or more, maintain field dampness 20 days or more, according to arsenic in soil difference pollution level, repeat spraying microbial inoculum
Number 4-10 times, will pour water, and keep soil surface ponding 10cm or more immediately after spraying microbial inoculum every time, and kept for 20 days or more
Dampness, in favor of microbial nutrition body in microbial inoculum and gemma is proliferated in the soil, biological oxidation and/or reduction arsenic become first
The gases such as base arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride.
2. a kind of arsenic in soil pollution ecology restorative procedure and application according to claim 1, it is characterised in that the method is
500-2000 grams of 100-900 grams of starch, rice and/or wheat stalk powder, 60-300 are added in 10 liters of bioreactors
Gram beancake powder, 40-180 grams of dipotassium hydrogen phosphate, 30-160 grams of potassium dihydrogen phosphate, 10-120 grams of calcium carbonate powder, 5-60 grams of sulfuric acid
Ferrous iron, 10-50 grams of magnesium sulfate, 7000 milliliters of water start blender and are stirred solution, start heating steam pipe, heating mixing
Solution, lower 126 DEG C of 0.1Mpa are kept for 45 minutes, and sterilizing terminates, and is cooled to the mould of 30 DEG C of inoculations 0.5-7% (mass/volume)
Bacterium keeps stirring, and 50-120 revs/min of agitator speed, ventilatory capacity 0.5-1 goes up to the air gas/liter liquid (volume/volume/minute),
Constant temperature is mixed 72-120 hours, and fermentation ends put tank.Same method is distinguished in above-mentioned same liquid fermentation medium
It is inoculated with aspergillus niger, the sickle-like bacteria of 0.5-7% (mass/volume), blender is started, 50-120 revs/min of agitator speed, ventilates
0.5-1 lift-off gas/liter liquid (volume/volume/minute) is measured, constant temperature is mixed 72-96 hours, and fermentation ends put tank.
3. a kind of arsenic in soil pollution ecology restorative procedure and application according to claim 1, it is characterised in that in the method,
By above-mentioned a treated 3 kinds of different fermentation liquids concentrations, filtering retains filtrate, keep viable bacteria and spore content in filtrate >=
3 kinds of bacterium solutions are mixed into composite bacteria liquid preparation by 0.5 hundred million/milliliter in equal volume.
4. a kind of arsenic in soil pollution ecology restorative procedure and application according to claim 1, it is characterised in that the method, it will
Treated that composite bacteria agent sprays agricultural land soil surface by above-mentioned b, before administration, ploughs soil, uniformly sprays composite bacteria agent per acre
50-200 kilograms, it is primary to plough soil, pours water immediately, so that soil surface has ponding 10cm or more, and keeps soil surface damp
Wet condition 20 days or more, 50-200 kilograms of composite bacteria agent is sprayed for the second time, and pour water immediately, so that agricultural land soil surface layer ponding
10cm or more, maintain field dampness 20 days or more, according to arsenic in soil difference pollution level, repeat spraying microbial inoculum number 4-10
It is secondary, it will pour water immediately after spraying microbial inoculum every time, and keep soil surface ponding 10cm or more, and holding 20 days or more moist shapes
State, in favor of microbial nutrition body in microbial inoculum and gemma are proliferated in the soil, biological oxidation and/or reduction arsenic become methyl arsenic,
The gases such as dimethyl arsenic, arsenic trimethide and arsenic hydride.
5. any a kind of improvement arsenic in soil method for governing pollution of Claims 1 to 4 and its in farmland As polluted soil, rubbish
Landfill yard, municipal sludge, gas station, stope, electroplate factory, electrolysis plant, metallurgical works, contaminated soil of site pollution arsenic dye are repaired
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