CN106244154A - A kind of Lead Pollution in Soil renovation agent - Google Patents

A kind of Lead Pollution in Soil renovation agent Download PDF

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Publication number
CN106244154A
CN106244154A CN201511006319.6A CN201511006319A CN106244154A CN 106244154 A CN106244154 A CN 106244154A CN 201511006319 A CN201511006319 A CN 201511006319A CN 106244154 A CN106244154 A CN 106244154A
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Prior art keywords
soil
strain
renovation agent
preparation
content
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CN201511006319.6A
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Inventor
梁茂林
郑才彬
柳正富
陆薇
申永刚
梁辉林
张寿坤
孙明阳
梁宏孺
吴春鹏
黄金荣
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Guangxi Hui Tai Environmental Protection Technology Co Ltd
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Guangxi Hui Tai Environmental Protection Technology Co Ltd
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Priority to CN201511006319.6A priority Critical patent/CN106244154A/en
Publication of CN106244154A publication Critical patent/CN106244154A/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/08—Reclamation of contaminated soil chemically
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C1/00—Reclamation of contaminated soil
    • B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K17/00—Soil-conditioning materials or soil-stabilising materials
    • C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
    • B09C—RECLAMATION OF CONTAMINATED SOIL
    • B09C2101/00—In situ
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K2101/00—Agricultural use

Abstract

The invention discloses a kind of Lead Pollution in Soil renovation agent, described renovation agent includes the water preparation that stabilizer, organic materials, biological bacteria preparation are made, described stabilizer includes bentonite, kieselguhr, hydroxyapatite, calcium magnesium phosphate, the powder that siliceous fertilizer is made, described biological bacteria preparation is made for the preparation of HT flora.The renovation agent together made by chemistry and biological method, it is possible to allow the soil environment quality after repairing meet the requirement of China's " standard of soil environment quality " (GB15618 1995) secondary standard value.And medium component is the most cheap and easy to get needed for composition used by chemical powder and microorganism fungus kind and microorganism, processing cost is low.In operation, simple and convenient, it is simple to promotion and implementation.

Description

A kind of Lead Pollution in Soil renovation agent
Technical field
The invention belongs to soil pollution by heavy metal recovery technique field, be specifically related to a kind of Lead Pollution in Soil renovation agent.
Background technology
Heavy metal generally refers to the proportion about 60 kinds of elements more than 4.0 or the proportion about 45 kinds of units more than 5.0 Element.It is significant that heavy metal in Study on environmental pollution is primarily referred to as the bio-toxicity such as Pb, Cd, Hg, Cr and metalloid As Element, also includes having the elements such as the heavy metal Zn of certain toxicity, Cu, Co, Ni and Sn.Some heavy metal element be human body and The element that other organism is required, but its concentration exceedes certain limit and will cause poisoning.
Soil pollution by heavy metal refers to that heavy metal is inputted in soil by mankind's activity, causes heavy metal content in soil the highest In background content, and cause the phenomenon that eco-environmental quality deteriorates.Investigation display, You Jin40% farmland, urban, Pearl River Delta dish Ground soil pollution by heavy metal exceeds standard, and wherein 10% belongs to severe overweight.Decades, Qi Zhongqian typically can be remained at heavy metal in soil 100 years can be remained.
The cardinal principle of heavy-metal contaminated soil restorative procedure has two kinds at present: change heavy metal existence shape in soil Formula, reduces its toxicity；Heavy metal in soil is removed.
The repairing and treating of heavy metal pollution agricultural land soil have multiple by way of.One is engineering control, such as, dig, soil removal and replacement, but Implement complexity, costly and easily reduce soil fertility.Two is chemical harnessing, and effect and expense are the most moderate, and shortcoming is the most once again Activation.Three is that agricultural is administered, and easily operation expends relatively low, but the cycle is long, and effect is the most notable.
Traditional modifying agent such as phosphate, Calx, flyash, peat etc., cost is high, to a single huge sum of money in repair process The repairing effect belonging to contaminated soil is obvious, and for several or various heavy contamination phenomenon repairing effect is the most notable. Traditional fixative can produce secondary pollution, and when edaphic condition changes, its stability is also affected.Such as patent Shen Please number be that the Chinese patent application of 201310589410. X proposes a kind of soil heavy mental adsorbent, be mainly used in absorption weight Cadmium metal, reduces the root system of plant absorption for cadmium element, but its technique relatively costly, that process needs more precisely just can reach To preferable effect.
In remediation of heavy metal-contaminated soil, it is numerous and diverse to there is equipment in great majority, and cost is high, processes the time long, is difficult to In execute-in-place and cause the unfavorable factors such as secondary pollution, it is difficult to obtain the conscious popularization of people in the practice of pollution control And application.
The super enriching plant repairing method of prior art, the time not only needed is long, and heavy metal super-enriched plant is difficult to Surviving, the later stage also wants heavy metal enriching plant to focus on etc., makes plant treatment method cost height, is difficult to promote.As: Peng Gram thrifty show that Herba Potamogetonis Pectinati effective can remove cadmium and lead from solution, it is possible to its dry sample is removed in industry as adsorbent Cadmium and lead.Pan Zhiming etc. use orthogonal experiment to carry out potted plant to Rhizoma Nephrolepis Cordifoliae (Herba Nephrolepis Cordifoliae), by entering cadmium in Rhizoma Nephrolepis Cordifoliae (Herba Nephrolepis Cordifoliae) root, stem and leaf series of samples Row is analyzed, and finds that Rhizoma Nephrolepis Cordifoliae (Herba Nephrolepis Cordifoliae) has preferable enrichment to cadmium.Cadmium wastewater is carried out 7-10 days supporting by the Herba Eichhorniae such as Zhang Zhijie Growing purification, clearance is up to 62%-89%.Liu Wei et al., by field investigation and greenhouse test, finds and confirms Viola baoshanensis (Viola baoshanensis) is the plant that a kind of cadmium is very capable.Prove that common violet can be with the absorption cadmium of excess.Wu Shuan Fructus Persicae research shows, Canna generalis Bailey can take away substantial amounts of cadmium from soil, and it is suitable for planting in low concentration pollution soil, at cadmium pollution ring The reparation aspect in border has a good application prospect.1989, Baker was found that sky blue brown blue dish in Midwest, Europe, a kind of Cd content can be enriched with and be up to the crucifer of 2130mg/kg.Compared with traditional restorative procedure, phytoremediation has green Color, environmental protection, economic dispatch advantage.But phytoremediation is the most under test, because of conditions such as soil, environment, weather, sunshines The impact of heavy metal super enriching plant is relatively big, is difficult to carry out the most large-area repair on the spot, it is difficult to industrialization The soil of formal layout heavy metal pollution.
Chemical leaching method and heavy metal deactivation method, the cost not only needed is high, but also introduces new in soil Material easily causes secondary pollution, and the physical arrangement of soil also can be greatly affected, and causes damage the vigor of soil.
Electrochemistry rule is the most immature due to its technology, is difficult to carry out in engineering, and expensive.Soil moved in improve the original method is right The respite of contaminated soil, the expense of needs is extremely expensive, there is also the risk polluteing subsoil water, while it is desirable to time Few.
Simple microbial method, compared with conventional physical chemical repair method, microorganism remediation engineering is simple, processing cost Relatively low, soil fertility and metabolic activity are negatively affected little, can avoid because of pollutant transfer, human health and environment being produced Raw impact.But, although microorganism remediation technology draws attention the most day by day, taking Suitable strains owing to being difficult to seek, great majority are reported The repairing method of microorganism effect in road is not notable, it is slow to take effect, repairing effect is unstable, limits the popularization and application of this technology.
Summary of the invention
The present invention is directed to the deficiencies in the prior art and provide a kind of soil utilizing chemical agent and microbial bacterial agent to combine Earth lead contamination renovation agent and using method thereof.
The technical solution used in the present invention is: a kind of Lead Pollution in Soil renovation agent, it is characterised in that: include stabilizer, The water preparation that organic materials, biological bacteria preparation are made, described stabilizer includes bentonite, kieselguhr, hydroxyapatite, calcium and magnesium phosphorus Fertilizer, the powder that siliceous fertilizer is made, described biological bacteria preparation is made for the preparation of HT flora.
Further: described bentonite 10-40%, kieselguhr 10-30%, hydroxyapatite 10-25%, calcium magnesium phosphate 5- 10%, siliceous fertilizer 5-20%, the preparation 10% of organic materials 20-45%, HT flora.
Further: described HT flora is HT2 strain, HT6 strain, HT9 strain, HT8 strain, the mixing of HT16 strain Body, the ratio of its mass percent is HT2 strain: HT6 strain: HT9 strain: HT8 strain: HT16 strain=2:1:3:3:1.
Further: the content of the viable bacteria of HT2 strain is 2-5 × 1010The content of the viable bacteria of individual/mL, HT6 strain is 1-3 ×1011The content of the viable bacteria of individual/mL, HT9 strain is 4-6 × 1010The content of the viable bacteria of individual/mL, HT8 strain is 3-5 × 1010 Individual/mL, the content of the viable bacteria of HT16 strain is 1-3 × 1011Individual/mL.
Further: HT2 strain is Bacillus alcaligenes, HT6 strain is pseudomonas, and HT8 strain is sulfur Ferrobacillus, HT9 bacterium Planting is thiophilic hydrochlorate antibacterial, and HT16 strain is Bacillus licheniformis.
Further: a kind of method administering heavy-metal contaminated soil, comprise the following steps:
1) analyze soil property, pollutant kind and pollution level, determine the concrete formula of renovation agent；
2) being collected contaminated soil, screen out the impurity such as stone, glass, tile, obtaining water content after crushing is 20- The pretreatment soil of 50%；
3) by bentonite 10-40%, kieselguhr 10-30%, hydroxyapatite 10-25%, calcium magnesium phosphate 5-10%, siliceous fertilizer 5-20%'s Powder mixes after putting into container as biological preparation as stabilizer, the water preparation of the preparation 10% of organic materials 20-45%, HT flora Make renovation agent;
4) in soil: pretreatment soil is fully mixed by the ratio of renovation agent=1:0.02-0.06 with renovation agent；
5) soil after covering treatment, maintenance about month, make biochemical complex stabilizer fully react with heavy metal ion, make Micropopulation therein plays one's part to the full, and general chemical reagent action time is 3-5 days, and the action time of biological reagent is 15-30 days；
6) sampling detection soil in heavy metal, detect qualified up to standard after, backfill or fill.
Further: described stabilizer is bentonite 10-45%, kieselguhr 15-35%, hydroxyapatite 10-30%, calcium and magnesium Phosphate fertilizer 5-10%, siliceous fertilizer 10-20% organic materials 30-50%.
Present invention also offers the culture medium of a kind of Bacillus alcaligenes, composed of the following components: sterilized water, glucose, (NH4) 2SO4 、Na2HPO4·12H2O ；KH2PO4、MgSO4, described sterilized water: glucose: (NH4)2SO4 : Na2HPO4·12H2O: KH2PO4: MgSO4Mass ratio be, 1000:10:0.75:2:0.65:0.2.
Further: the pH of described preparation is 7.0, sterilizing 20min under conditions of 115 DEG C again after mixing.
Present invention also offers the culture medium of a kind of sulfur Ferrobacillus, composed of the following components: sterilized water, FeSO4, (NH4)2SO4、KCl、K2HPO4、MgSO4, Ca(NO3), H2SO4, described sterilized water: FeSO4: (NH4)2SO4: KCl, K2HPO4: MgSO4: Ca (NO3)2: H2SO4Mass ratio be, 1000:44.1:3:0.1:0.5:0.5:0.01:0.49.
Present invention also offers the culture medium of a kind of thiophilic hydrochlorate antibacterial, composed of the following components: NH4Cl、 NaCl、 K2HPO4 、Na2SO4、MgSO4·7H2O 、CaCl2、FeSO4(NH4)(SO4)2·6H2O, lactic acid, yeast extract, described: NH4Cl: NaCl:K2HPO4: Na2SO4: MgSO4·7H2O: CaCl2: FeSO4(NH4)(SO4)2·6H2O, lactic acid, the mass ratio of yeast extract For, 1:2:0.5:0.5:2:0.1:0.5:6.0:1.
The invention has the beneficial effects as follows:
1. individually all there is problem more or less, such as chemical reagent instant effect in chemical reagent or microbial bacterial agent, but It is not thorough, easily causes secondary pollution；Microbial bacterial agent, takes effect slow, and microorganism is difficult in soil cultivation etc. of surviving.And both Combination then can be complementary, thorough by stay after chemical redemption, can be solved by microorganism.Slow the lacking that take effect of microorganism Point, then can first repair major part by chemical redemption, and soil is improved, and microorganism can also be conducive at contaminated soil simultaneously In growth and breeding.The complex utilizing the two can be to foreshorten to 1-2 month repair time.
2. the water preparation that the powder that chemical reagent is made and microbial bacterial agent are made is fabricated separately, and applies when processing simultaneously, Play the most maximum effect, be simultaneously introduced Organic substance and provide nutrient for microorganism formulation, provide favourable ring for dispelling heavy metal Border.Chemical reagent and microbial bacterial agent jointly act on and shorten the response time.Both apply jointly, do not interfere with the life of microorganism Long breeding, the beneficially reparation of heavy metal contaminated soil.Under the common effect of chemicals and biological preparation, effectively reduce The toxicity of heavy metal in soil, significantly reduces the intake of plant heavy metal, repairs, improves contaminated by heavy metals soil Earth, protects environment.Use this soil-repairing agent can improve soil constantly, increase the soil organism, improve soil water-retaining and protect Fertilizer and venting capability, promote plant growth.
3. chemical reagent chemical stabilizer therein can with activated state heavy metal ion generation mineralising react generation primary State mineral substance compound, phosphorio compound can form inert precipitate with heavy metal and effectively reduce heavy metal activity, clay pit The high reaction table area of species medicament utilization itself catches heavy metal ion, and heavy metal ion more likely travels further into clay pit In the mineral structure lattice of thing itself, form stable mineral shape.Microorganism produces in various growth metabolism active procedures Multiple low molecular organic acids, the secondary metabolite such as enzyme can be direct or indirect dissolving heavy metal or heavy metallic mineral.Micro- The reactions such as biological cell can adsorb with heavy metal ion, complexation, precipitation, oxidoreduction, change the existence of variable valency metal 6 valency chromium ions price reduction are such as that 4 valencys, divalent are even reduced into metallic state by state, it is achieved heavy metal ion innoxious and Fixing, the cell wall of microorganism can also be by heavy metal chelating at cell surface.
4. Bacillus alcaligenes, pseudomonas, bacillus cereus can be converted into Cr3+ Cr6+, and sulfur Ferrobacillus can aoxidize As, Cu, Mo, Fe etc., pseudomonas can make As, and Fe, Mn etc. occur biological oxidation, reduce the activity of these heavy metals.Thiophilic hydrochlorate is thin Bacterium can be by sulfate reduction sulphidisation.
5., under both comprehensive functions, reduce bio-absorbable effectiveness and the soil extract toxicity of heavy metal, prevent Heavy metal ion migrates with subsoil water and surface water, it is to avoid heavy metal ion harm environmental and human health impacts.Chemical reagent and soil Heavy metal in earth can quickly react, the lag phase of microorganism when, and the huge sum of money that can react with chemical reagent Belonging to stabilized or solidify, now soil environment is the most tentatively improved, and microorganism can utilize organic substance growth and breeding therein, During growth and breeding, produce the metabolites such as enzyme and produce secondary metabolite such as organic acid etc., microorganism at stable phase Cell continues heavy metal generation effect remaining in soil.Obtain more preferable treatment effect.
6. raw material sources are extensively easy to get, and simple to operate easily execute.
7. effect of the biochemical existing chemical stabilizer of complex repairation agent of the present invention has again microbial bacterial agent heavy metal soil The repairing effect that earth pollutes.Under both dual functioies, heavy-metal contaminated soil obtains repairing the most thoroughly.
8. needed for composition used by chemical powder and microorganism fungus kind and microorganism, medium component is the most cheap and easy to get, place Reason low cost.In operation, simple and convenient, it is simple to promotion and implementation.Soil environment quality after reparation meets China's " soil environment Quality standard " requirement of (GB15618-1995) secondary standard value.
9. general chemical reagent action time is 3-5 days, and the action time of biological reagent is 15-30 days.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described:
The explanation in the raw materials used source of following example:
HT2 strain is Bacillus alcaligenes, and HT6 strain is pseudomonas, and HT8 strain is sulfur oxide Ferrobacillus, and HT9 strain is microsphere Bacterium, HT16 strain is Bacillus licheniformis.Wherein the content of the viable bacteria of HT2 strain is 2-5 × 1010The work of individual/mL, HT6 strain The content of bacterium is 1-3 × 1011The content of the viable bacteria of individual/mL, HT9 strain is 4-6 × 1010Containing of the viable bacteria of individual/mL, HT8 strain Amount is 3-5 × 1010Individual/mL, the content of the viable bacteria of HT16 strain is 1-3 × 1011Individual/mL.
HT2 strain is Bacillus alcaligenes, Latin literary fame: Alcaligenes Faecalis Castellani et Chalmers, microbial preservation number: ATCC 31555, Guangdong Province's Culture Collection.Its culture medium is: glucose 10g；(NH4)2SO40.75g；Na2HPO4·12H2O 2g；KH2PO40.65g；MgSO40.2g；Sterilized water 1000ml； pH7.0；115 DEG C of sterilizing 20min.
HT6 strain from China DSMZ, preserving number MCCC1A00767.HT6 strain (pseudomonas) culture medium For: peptone 20.0g/L, potassium sulfate 10.0g/L, magnesium chloride 1.4g/L, agar 15.0g/L, cultivate by pH7.2 ± 0.2.36 DEG C 18-24℃.HT16 strain (Bacillus licheniformis) seed culture medium: Carnis Bovis seu Bubali cream 5g, soy peptone 10g, Nacl 5g, water 1L, pH7.0.After activation, viable bacteria content is 5 × 1010Individual/mL.
HT8 strain, sulfur oxide Ferrobacillus, Latin literary fame: Thiobacillus ferrooxidans, microbial preservation number: CCTCC M 204057, China typical culture collection center.Its culture medium is: FeSO444.1g, (NH4)2SO43.0g, KCl 0.1g, K2HPO40.5g, MgSO40.5g, Ca(NO3) 2 0.01g, 5mol/L H2SO4 1.0 mL, H2O 1000mL。
HT9 strain, micrococcus luteus, Latin is entitled: Micrococcus sp., is preserved in Chinese Sea microorganism fungus kind and protects Hiding administrative center, microbial preservation number: 1A00764, its culture medium is: NaCl 2g, NH4Cl 1g, K2HPO40.5g, Na2SO4 0.5 g, MgSO4·7H2O 2g, CaCl20.1g, FeSO4(NH4)(SO4)2·6H2O 0.5g, 70% lactic acid 5mL, yeast extract 1g, The agar that solid medium then adds 1%.After obtaining strain, amplification culture, viable bacteria content is about 6 × 1010Individual/mL.
HT16 strain, Bacillus licheniformis, Latin literary fame: Rhodococcus equi, from DSMZ of China, Preserving number is: ACCC10236.
Embodiment 1:
The concrete operation step of heavy metal-polluted soil renovation agent rehabilitating soil is as follows:
1) sample analysis soil property, pollutant kind and pollution level, determines the concrete formula of renovation agent；
2) being collected contaminated soil, screen out the impurity such as stone, glass, tile, obtaining water content after crushing is 20% Pretreatment soil；
3) by bentonite 1000kg, kieselguhr 1000kg, hydroxyapatite 1000kg, calcium magnesium phosphate 500kg, siliceous fertilizer 1000kg Powder put into container as stabilizer, the water preparation of the preparation 1000kg of organic materials 4500kg, HT flora as biological preparation Renovation agent is made in rear mixing;
4) in soil: pretreatment soil and renovation agent are fully uniformly mixed so as to obtain the soil after process by the ratio of renovation agent=1:0.06；
5) on the concrete floor after sterilization of the soil heap after processing, covered with plastic film carries out maintenance, at room temperature maintenance 18 My god, make biochemical complex stabilizer fully react with heavy metal ion so that it is in micropopulation play one's part to the full.
6) sampling detection soil in heavy metal, detect qualified up to standard after, backfill or fill.
Embodiment 2
1) sample analysis soil property, pollutant kind and pollution level, determines the concrete formula of renovation agent；
2) being collected contaminated soil, screen out the impurity such as stone, glass, tile, obtaining water content after crushing is 50% Pretreatment soil；
3) by bentonite 4000kg, kieselguhr 1000kg, hydroxyapatite 1000kg, calcium magnesium phosphate 500kg, siliceous fertilizer 500kg's Powder is as stabilizer, after the water preparation of the preparation 1000kg of organic materials 2000kg, HT flora puts into container as biological preparation Renovation agent is made in mixing;
4) in soil: pretreatment soil and renovation agent are fully uniformly mixed so as to obtain the soil after process by the ratio of renovation agent=1:0.02；
5) on the concrete floor after sterilization of the soil heap after processing, covered with plastic film carries out maintenance, at room temperature maintenance 35 My god, make biochemical complex stabilizer fully react with heavy metal ion so that it is in micropopulation play one's part to the full.
6) sampling detection soil in heavy metal, detect qualified up to standard after, backfill or fill.
Embodiment 3
1) sample analysis soil property, pollutant kind and pollution level, determines the concrete formula of renovation agent；
2) being collected contaminated soil, screen out the impurity such as stone, glass, tile, obtaining water content after crushing is 30% Pretreatment soil；
3) by bentonite 1000kg, kieselguhr 1000kg, hydroxyapatite 2500kg, calcium magnesium phosphate 1000kg, siliceous fertilizer 1000kg, powder put as biological preparation as stabilizer, the water preparation of the preparation 1000kg of organic materials 2500kg, HT flora After entering container, renovation agent is made in mixing;
4) in soil: pretreatment soil and renovation agent are fully uniformly mixed so as to obtain the soil after process by the ratio of renovation agent=1:0.05；
5) on the concrete floor after sterilization of the soil heap after processing, covered with plastic film carries out maintenance, at room temperature maintenance 20 My god, make biochemical complex stabilizer fully react with heavy metal ion so that it is in micropopulation play one's part to the full.
6) sampling detection soil in heavy metal, detect qualified up to standard after, backfill or fill.
Embodiment 4
1) sample analysis soil property, pollutant kind and pollution level, determines the concrete formula of renovation agent；
2) being collected contaminated soil, screen out the impurity such as stone, glass, tile, obtaining water content after crushing is 40% Pretreatment soil；
3) by bentonite 1000kg, kieselguhr 3000kg, hydroxyapatite 2000kg, calcium magnesium phosphate 500kg, siliceous fertilizer 500kg Powder put into container as stabilizer, the water preparation of the preparation 1000kg of organic materials 2000kg, HT flora as biological preparation Renovation agent is made in rear mixing;
4) in soil: pretreatment soil and renovation agent are fully uniformly mixed so as to obtain the soil after process by the ratio of renovation agent=1:0.04；
5) on the concrete floor after sterilization of the soil heap after processing, covered with plastic film carries out maintenance, at room temperature maintenance 28 My god, make biochemical complex stabilizer fully react with heavy metal ion so that it is in micropopulation play one's part to the full.
6) sampling detection soil in heavy metal, detect qualified up to standard after, backfill or fill.
Embodiment 5
1) sample analysis soil property, pollutant kind and pollution level, determines the concrete formula of renovation agent；
2) being collected contaminated soil, screen out the impurity such as stone, glass, tile, obtaining water content after crushing is 35% Pretreatment soil；
3) by bentonite 1000kg, kieselguhr, 10-30%, hydroxyapatite, 10-25%, calcium magnesium phosphate 500kg, siliceous fertilizer 2000kg, powder as stabilizer, the preparation 1000kg of organic materials 3500kg, HT flora, the water preparation of 10% is as biology system After container is put in agent, renovation agent is made in mixing;
4) in soil: pretreatment soil and renovation agent are fully uniformly mixed so as to obtain the soil after process by the ratio of renovation agent=1:0.03；
5) on the concrete floor after sterilization of the soil heap after processing, covered with plastic film carries out maintenance, at room temperature maintenance 21 My god, make biochemical complex stabilizer fully react with heavy metal ion so that it is in micropopulation play one's part to the full.
The embodiment of the present invention 1 is also also being tested in the heavy metal pollution of Guangxi Zhuang Autonomous Region county.Experimental technique: Gather soil, survey its heavy metal in soil kind and content.The renovation agent using embodiment 1 to make, mixes renovation agent and soil Closing uniformly, placement processes 30 days.Start every 5 days detection once its content of beary metal.After 10 days, detection in every 2 days is once.
Result is as follows:
Lead (Pb) (mg/kg) Arsenic (As) (mg/kg) Cadmium (Cd) (mg/kg) Hydrargyrum (Hg) (mg/kg)
Before process 1100 95 2.0 1.5
5th day 880 74.1 1.4 1.13
10th day 715 55.1 1.0 0.79
12nd day 605 45.6 0.8 0.64
14th day 550 40.1 0.71 0.56
16th day 462 37.8 0.64 0.50
18th day 385 34.1 0.58 0.48
20th day 319 31.5 0.51 0.42
22nd day 305 29.0 0.49 0.37
24th day 275 25.6 0.47 0.35
26th day 251 23.7 0.45 0.32
28th day 234 21.2 0.42 0.31
30th day 220 19 0.40 0.30
The content of lead reaches the requirement of China's " standard of soil environment quality " (GB15618-1995) secondary standard value.
Above content is to combine concrete preferred implementation further description made for the present invention, it is impossible to assert Being embodied as of the present invention is confined to these explanations.For general technical staff of the technical field of the invention, On the premise of present inventive concept, it is also possible to make some simple deduction or replace, all should be considered as belonging to the present invention's Protection domain.

Claims (10)

1. a Lead Pollution in Soil renovation agent, it is characterised in that: include the water that stabilizer, organic materials, biological bacteria preparation are made Agent, described stabilizer is bentonite, kieselguhr, hydroxyapatite, calcium magnesium phosphate, the powder that siliceous fertilizer is made, described biological bacteria system Agent is made for the preparation of HT flora.
2. Lead Pollution in Soil renovation agent as claimed in claim 1, it is characterised in that: described HT flora is HT2 strain, HT6 bacterium Kind, HT9 strain, HT8 strain, the mixture of HT16 strain, the ratio of its mass percent is HT2 strain: HT6 strain: HT9 bacterium Kind: HT8 strain: HT16 strain=2:1:3:3:1.
3. Lead Pollution in Soil renovation agent as claimed in claim 1 or 2, it is characterised in that the content of the viable bacteria of HT2 strain is 2-5 ×1010The content of the viable bacteria of individual/mL, HT6 strain is 1-3 × 1011The content of the viable bacteria of individual/mL, HT9 strain is 4-6 × 1010 The content of the viable bacteria of individual/mL, HT8 strain is 3-5 × 1010The content of the viable bacteria of individual/mL, HT16 strain is 1-3 × 1011Individual/ mL。
4. Lead Pollution in Soil renovation agent as claimed in claim 1 or 2, it is characterised in that: HT2 strain is Bacillus alcaligenes, HT6 bacterium Planting is pseudomonas, and HT8 strain is sulfur Ferrobacillus, and HT9 strain is thiophilic hydrochlorate antibacterial, and HT16 strain is Bacillus licheniformis.
5. the method administering heavy-metal contaminated soil, comprises the following steps:
1) analyze soil property, pollutant kind and pollution level, determine the concrete formula of renovation agent；
2) being collected contaminated soil, screen out the impurity such as stone, glass, tile, obtaining water content after crushing is 20- The pretreatment soil of 50%；
3) by bentonite 10-40%, kieselguhr 10-30%, hydroxyapatite 10-25%, calcium magnesium phosphate 5-10%, siliceous fertilizer 5-20%'s Powder mixes after putting into container as biological preparation as stabilizer, the water preparation of the preparation 10% of organic materials 20-45%, HT flora Make renovation agent;
4) in soil: pretreatment soil is fully mixed by the ratio of renovation agent=1:0.02-0.06 with renovation agent；
5) soil after covering treatment, maintenance 18-35 days；
6) sampling detection soil in heavy metal, detect qualified up to standard after, backfill or fill.
6. the Lead Pollution in Soil renovation agent as described in claim 1 or 6, by mass percentage, including following components: bentonite 10-40%, kieselguhr 10-30%, hydroxyapatite 10-25%, calcium magnesium phosphate 5-10%, siliceous fertilizer 5-20%, organic materials 20-45%, The preparation 10% of HT flora.
7. the culture medium of a Bacillus alcaligenes, it is characterised in that composed of the following components: sterilized water, glucose, (NH4)2SO4 、 Na2HPO4·12H2O ；KH2PO4、MgSO4, described sterilized water: glucose: (NH4)2SO4 : Na2HPO4·12H2O:KH2PO4: MgSO4Mass ratio be, 1000:10:0.75:2:0.65:0.2.
8. culture medium as claimed in claim 1, it is characterised in that: the pH of described preparation is 7.0, the bar of 115 DEG C again after mixing Sterilizing 20min under part.
9. the culture medium of a sulfur Ferrobacillus, it is characterised in that composed of the following components: sterilized water, FeSO4, (NH4)2SO4、 KCl、K2HPO4、MgSO4, Ca(NO3), H2SO4, described sterilized water: FeSO4: (NH4)2SO4: KCl, K2HPO4: MgSO4: Ca (NO3)2: H2SO4Mass ratio be, 1000:44.1:3:0.1:0.5:0.5:0.01:0.49.
10. the culture medium of a thiophilic hydrochlorate antibacterial, it is characterised in that composed of the following components: NH4Cl、 NaCl、K2HPO4 、 Na2SO4、MgSO4·7H2O 、CaCl2、FeSO4(NH4)(SO4)2·6H2O, lactic acid, yeast extract, described: NH4Cl:NaCl: K2HPO4: Na2SO4: MgSO4·7H2O: CaCl2: FeSO4(NH4)(SO4)2·6H2O, lactic acid, the mass ratio of yeast extract be, 1: 2:0.5:0.5:2:0.1:0.5:6.0:1.
CN201511006319.6A 2015-12-29 2015-12-29 A kind of Lead Pollution in Soil renovation agent Pending CN106244154A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106734138A (en) * 2017-03-22 2017-05-31 刘育含 A kind of method of remediating lead-contaminated soil
CN107971336A (en) * 2017-12-11 2018-05-01 西南科技大学 A kind of Lead Pollution in Soil biology in situ renovation method
CN109926445A (en) * 2017-12-19 2019-06-25 国家电投集团远达环保工程有限公司重庆科技分公司 A kind of soil remediation method
CN110586642A (en) * 2019-09-10 2019-12-20 铜仁市万山区植保植检站 Method for repairing lead-cadmium contaminated soil

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106734138A (en) * 2017-03-22 2017-05-31 刘育含 A kind of method of remediating lead-contaminated soil
CN107971336A (en) * 2017-12-11 2018-05-01 西南科技大学 A kind of Lead Pollution in Soil biology in situ renovation method
CN109926445A (en) * 2017-12-19 2019-06-25 国家电投集团远达环保工程有限公司重庆科技分公司 A kind of soil remediation method
CN110586642A (en) * 2019-09-10 2019-12-20 铜仁市万山区植保植检站 Method for repairing lead-cadmium contaminated soil
CN110586642B (en) * 2019-09-10 2021-06-29 铜仁市万山区植保植检站 Method for repairing lead-cadmium contaminated soil

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