CN108941193A - A kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application - Google Patents
A kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application Download PDFInfo
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- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
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Abstract
A kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application, the technology belong to ecological environmental protection field.The technology is realized by following steps: inoculation fermentation composite bacteria agent first, then applies microbial inoculum in crop field, while planting Sedum alfredii Hance and rich arsenic fern.Starch is added in the bioreactor, soya-bean cake, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium carbonate, ferrous sulfate etc., add enough water, stirring, it mixes, heat sterilization, it is cooling, it is inoculated with the strains such as thermophilic hot rod bacterium, fermentation, it is cooling, mixture, it is canned, in being manured into soil, naturally culture 8-20 months, Sedum alfredii Hance can draw zinc cadmium lead etc. from soil, rich arsenic fern can absorb arsenic from soil, compound bacteria can continue to convert escaping gas for the arsenic in soil, it is evaporate into air from soil, to remove the arsenic zinc cadmium lead in soil, eliminate the pollution such as soil and groundwater arsenic zinc cadmium lead, soil protection environment, soil protection production and ecological functions, protect groundwater environment, it ensures food safety.
Description
Technical field
The invention belongs to ecological environmental protection field, in particular to a kind of arsenic in soil cadmium lead zinc combined pollution bioengineering is repaired
Compound method and application.
Background technique
Arsenic is a kind of environmental contaminants that toxicity is very strong.Arsenic in natural soils is mainly derived from soil parent material.By
During rock weathering forms soil, the content (relative to soil parent material) of arsenic has apparent enrichment.Mankind's activity such as mining,
It smelts, dirty filling, apply fertilizer, the application of insecticide and timber preservative all may cause soil arsenic pollution.Excessive Arsenic is accumulated in soil,
It will cause crop failure and quality decline, while may cause water source and atmosphere pollution, and the way such as food chain and water body can be passed through
Diameter enters human body.In recent years studies have shown that the arsenic of low amounts can also seriously endanger human health.
2016, it was one that " Soil Pollution Control action plan ", " Soil Pollution Control action plan ", which is formally issued and implemented,
The item huge soil pollution recovery project of scale of investment, also will be a long-term engineering.Due to soil remediation Its Relevant Technology Standards
The many difficulties implemented with soil remediations such as the shortages of basic data, interior in recent years, China's Soil Pollution Control also needs first to lead to
It crosses pilot demonstration and part is carried out, then realize the process of scale development.Basic data and the accumulation and reparation for repairing experience
The deposit of technology by be China's current stage soil remediation vital task.
China is arsenic minerals big country, arsenic minerals be widely distributed in the Central-South and southwestern Hunan in China, Yunnan, Guangxi, Guangdong etc. save,
Area.Arsenic minerals be easy to cause the pollution of neighbouring soil and water body in exploitation or smelting process, meanwhile, many accompanying elements such as zinc cadmium
Lead etc. is also released.According to Wang Zhengang etc. near Shimen Realgar Mine 3 villages investigate discovery, soil arsenic content be 84~
296mg/kg, this shows soil by different degrees of pollution.Arsenic can also pass through water body migration stain large area agricultural land soil.Cause
Paddy field mostly uses irrigation canals and ditches to irrigate, and the water paddy soil contamination phenomenon near mining and smeltery is on the rise, such as Hunan Changning
County has large area by arsenic polluted water rice field, and arsenic content is 92~840mg/kg；There are more than 8000 hectares of paddy fields by arsenic dirt in Hubei
Dye.Cause 70,000,000 people's drinking water arsenic contents exceeded since underground water is excessively high containing arsenic in Bangladesh；With this well water containing arsenic
Irrigation water rice field allows for 0~15cm soil horizon arsenic content in the time less than 10 years and is increased to 83mg/kg, and local native
Earth arsenic background value is only 4~8mg/kg.
The biological effect and its toxicity of arsenic, mainly due to sulfydryl (- SH), cystine and the half Guang ammonia in arsenic and zymoprotein
The amino (- N H) of sour sulfur-bearing has very strong affinity, so that histocyte breathing is obstructed in conjunction with after, mitochondrial swelling, to make generation
Thank stopping, cell death.For one kind, " the known mankind are carcinogenic by the World Health Organization (WHO) and U.S.EPA (EPA) deciding grade and level for arsenic
Substance ", human body exposes arsenic for a long time can lead to the canceration of the internal organs such as cutaneum carcinoma and kidney, liver, bladder.The approach of arsenic poisoning is main
Enter digestive system by drinking-water and food, the exhaust gas containing arsenic of industrial discharge enters the respiratory system.As3+ can be with human body enzyme
Sulfydryl combines, inhibitory enzyme activity, leads to carbohydrate metabolism disturbance, the content of acetone in blood and tissue increases and nerve endings function
It can disorder.As5+Toxic effect is slower, it can destroy mitochondria oxidative phosphorylation, phosphate can be replaced to generate unstable
Arsenate, it may appear that the sequelae such as polyneuritis, myelitis, hypoplastic anemia.The denaturant of adult arsenious acid
Amount is 5~50mg/kg, and lethal dose is 100~300mg/kg；The half lethal concentration (LD50) of arsenious acid is 10mg/kg, and arsenic
The LD50 of acid is 138mg/kg.
With the increase of arsenic amount, soil bacteria and actinomyces sum are substantially reduced, and arsenic is to Soil hydrolase system (urea
Enzyme, phosphatase and protease) and oxidoreducing enzyme (dehydrogenase, polyphenol oxidase and catalase) have different degrees of suppression
System.Arsenic inhibits CO in Soil Microorganism activity2Generation, significantly reduce the activity of denitrifying bacteria.Arsenic is to soil fixed nitrogen
Bacterium, phosphate-solubilizing bacteria and cellulose-decomposing bacteria have inhibiting effect, and wherein trichoderma and Bacterium anthracoides are most sensitive to arsenic, and Soybean Root
Tumor bacterium and the rigid spirillum patience highest containing rouge.
Arsenic pollution and arsenic species are a global problems.Japanese government is by arsenic poisoning and lead poisoning, minamata disease, bone
Seriously pollution disease is mutually arranged side by side for pain disease etc., is declared as fourth environmental disease.The toxicity of general arsenic is successively are as follows: and arsenic hydride > trivalent inorganic arsenic >
Pentavalent inorganic arsenic > organo-arsenic > element arsenic.Arsenic is plant non-essential element, in general plant As content mobility scale be 0.01~
5mg/kg.Plant can absorb arsenic from external environment in growth and development process, and the arsenic of low amounts can stimulate the growth of plant.But
Higher arsenic content can generate toxic effect to plant in soil, endanger its growth and development, make crop yield and quality decline.
The soil that property is different, purposes is different, pollution level is different, the technology and methods repaired are not also identical.It is common
Arsenic in soil cadmium lead zinc pollution improvement recovery technique has following several: immobilization, stabilization technology, passivating technique, Soil leaching skill
Art, electric repairing technique in situ, soil improvement by importing soil from other places, bioremediation technology etc..Fixation, Dunhua, stabilization technology are at low cost, but material
A large amount of uses of curing agent can destroy the structure of soil, and therefore, which is not suitable for the soil remediation of large area.Soil leaching
Technology is eluted with leacheate, and the method easily causes the leaching loss and precipitating of certain nutrients, and therefore, this method is suitable for area
The soil remediation of small heavy metal pollution.Ma et al. has found a kind of plant-Pteris vittata L in the middle part of Fla., can surpass
It is enriched with arsenic, Pteris vittata L is planted in laboratory, cultivates 6 weeks, the content of arsenic has reached 22630mg/kg in accessory pinna.Chen Tongbin etc.,
Wei Chaoyang etc. has also discovered the super enriching plant-Pteris vittata L and cretan brake of arsenic in Hunan China.Recently it was also discovered that very
More plants can be good at being enriched with arsenic, such as: Creeping bentgrass, Meng Tana chrysanthemum, knotweed vehicle, Bermuda grass etc..But these plants are big
Mostly it is shade plant, among long term growth virgin soil open country out of office, cultivates for large area agricultural land soil arsenic, be exposed to the sun intolerant to sunburst
With the great change of pH value, often undergrowth, repairing effect is bad, simultaneously because after continuous multi-generation vegetative propagation, super build-up properties
It substantially degenerates, loses repair function.
This technology tires out crop using multiple-microorganism mix bacterium agent-a variety of ultraproducts and combines rehabilitating soil arsenic cadmium lead zinc, in conjunction with
Soil moisture and oxidation-reduction potential regulation, restore and/or are oxidized to methyl arsenic, dimethyl for the compound of arsenic in soil and arsenic
Arsenic, arsenic trimethide and arsenic hydrogen Den gas, evaporate from soil, so that soil arsenic pollution is removed, and the huge sum of money such as cadmium lead zinc
Category is absorbed by hyperaccumulative plant, removes out soil, thus soil protection and groundwater environment, the composite highly effective produced using this law
Microbial inoculum treatment effeciency is high, at low cost, and environment friendly is high, easy to operate, without secondary pollution, heavy industrialization easy to accomplish
Using.
Summary of the invention
The technical issues of solution: the present invention is directed to arsenic in soil cadmium lead zinc pollution, especially agricultural land soil and underground water, metallurgy
Factory, metal stope and chemical plant contaminated sites invent a kind of high efficiency composition microbial inoculum and hyperaccumulative plant united repairing heavy metal
Contaminated soil, within a short period of time arsenic in soil cadmium lead zinc pollution major part bio-absorbable or gaseous volatilization, to remove soil
Harm of the refreshing cadmium lead zinc to environment and the mankind.
Technical solution: a kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application, step are a kind of soil
Earth arsenic cadmium lead zinc combined pollution bioengineering restorative procedure and application, it is characterised in that step are as follows: a. is in 10 liters of bioreactors
The middle starch for being added 100-700 grams, 60-300 grams of coarse colza meal, 40-130 grams of dipotassium hydrogen phosphate, 30-130 grams of potassium dihydrogen phosphate,
10-120 grams of calcium carbonate powder, 15-90 grams of ferrous sulfate, 10-80 grams of magnesium sulfate, 7000 milliliters of water start blender stirring, open
Dynamic heating steam pipe heats mixed solution, and lower 126 DEG C of 0.1Mpa are kept for 45 minutes, and sterilizing terminates, and is cooled to 30 DEG C of inoculation 0.5-
The thermophilic hot rod bacterium of 8% (mass/volume), stirring, 50-120 revs/min of agitator speed, ventilatory capacity 0.5-1 lift-off gas/liter
Liquid/minute, constant temperature are mixed 24-48 hours, and fermentation ends put tank.Same method is fermented in above-mentioned same liquid and is trained
Feeding base is inoculated with the Thiobacillus thioxidans of 0.5-8% (mass/volume), arsenic oxidizing bacteria, Streptomyces glaucoviolaceus, Dare Ford respectively
Bacterium, children set blastocyst, cultivate respectively, start blender, 50-120 revs/min of agitator speed, ventilatory capacity 0.5-1 go up to the air gas/
It rises liquid/minute, constant temperature is mixed 24-72 hours, and fermentation ends put tank.B. by above-mentioned a treated 6 kinds of different hairs
Zymotic fluid concentration, filtering retain filtrate, keep viable bacteria and spore content >=500,000,000/milliliter in filtrate, wherein Dare Ford bacterium, children's set
6 kinds of bacterium solutions are mixed into composite bacteria liquid preparation by blastocyst viable bacteria and spore content >=100,000,000/milliliter in equal volume.C. it deep ploughs to be repaired
Soil, by above-mentioned b, treated that composite bacteria agent sprays agricultural land soil surface, uniformly sprays composite bacteria agent 50-200 thousand per acre
Gram, it pours water, so that soil surface has ponding 10cm or more, and is kept for soil surface dampness 20 days or more, dries in the air naturally immediately
Then dry soil moisture plants Sedum alfredii Hance, rich arsenic fern and rich arsenic penny cress.D. soil surface using spacing in the rows × line-spacing=
15cm × 15cm transplants Sedum alfredii Hance, rich arsenic fern and penny cress seedling, can transplant seeding row spacing as the case may be with appropriate adjustment,
Sedum alfredii Hance and rich arsenic fern and penny cress interval are transplanted, i.e. Sedum alfredii Hance-richness arsenic fern-Sedum alfredii Hance-penny cress, plant finishes,
50-200 kilograms of composite bacteria agent is sprayed for the second time, and is poured water immediately, so that agricultural land soil surface layer is completely moist and by a small amount of ponding,
Moist 20 days of field or more is maintained, according to arsenic in soil pollution level, repeat spraying microbial inoculum number 4-10 times, after spraying microbial inoculum every time
It will pour water immediately, and keep soil surface completely wet, and kept for 20 days or more, so that hyperaccumulative plant survives and microbial inoculum
Middle microbial nutrition body and gemma are proliferated in the soil, biological oxidation and/or reduction arsenic become methyl arsenic, dimethyl arsenic, front three
The gases such as base arsenic and arsenic hydride.E. after the Sedum alfredii Hance of plant, rich arsenic fern, penny cress yield positive results, these plants can be gathered in
Object, soil of deep ploughing immediately continue to transplant Sedum alfredii Hance-richness arsenic fern-Sedum alfredii Hance-penny cress according to above-mentioned planting patterns, and spray
Composite bacteria agent is applied, is poured water immediately, and keeps different surface layers completely 20 days moist or more, can plant 2-3 times within 1 year, gather in 2-3
Secondary, harvested Sedum alfredii Hance, rich arsenic fern, penny cress etc. put together, and dry, and power transmission factory burns, or local centralized burning, ash
Cinder collects, and periodically send fixed-end forces company that the heavy metal in ashes is uniformly processed.It, can according to the concrete condition of soil pollution
Continuously to plant several years.
The composite bacteria agent of any a kind of rehabilitating soil arsenic cadmium lead zinc pollution and its in farmland, refuse landfill, city
Sludge, gas station, paper mill, stope, electroplate factory, nearby soil is dirty for electrolysis plant, metallurgical works, contaminated sites and farm
Contaminate the application that arsenic cadmium lead zinc dye is repaired.6 kinds of microorganisms can protect center from Chinese Academy of Sciences bacterium used in this law, the Chinese Academy of Agricultural Sciences bacterium protects
Center, Nanjing are happy to help ecological environment Science and Technology Ltd. to buy；3 kinds of hyperaccumulative plants used can therefrom section's barnyard reason institute,
Soil institute and Nanjing are happy to help ecological environment Science and Technology Ltd. to buy.
The utility model has the advantages that the present invention can eliminate arsenic cadmium lead zlnc pollutant in soil and groundwater to greatest extent, to greatest extent
Elimination arsenic in soil cadmium lead zinc pollution and its destruction to environment and the harm to the mankind, be conducive to Ecological Sustainable Development.
The present invention is compared with prior art:
1) the arsenic in soil cadmium lead zinc pollution composite bacteria agent and hyperaccumulative plant combined remediation technology researched and developed in the present invention, production
Method is easy, technically simple easy, strong operability, low in cost, practical；
2) present invention removes soil arsenic pollution using microbiological oxidation reduction technique, can be really soil and/or underground
Arsenic is converted into gaseous volatilization and goes out in water, reduces the arsenic polluted in soil to the maximum extent, fundamentally eliminates in soil
Arsenic；It is absorbed using 3 kinds of different hyperaccumulative plants from contaminated soil and removes heavy metal arsenic cadmium lead zinc etc., with currently used single
Solidification hair/deactivation method or microorganism/phytoremediation compare, be real removal arsenic in soil cadmium lead zinc pollution, residue and two
Secondary pollutant is seldom, selected 6 kinds of compound bacterias be all it is naturally occurring in soil natural circle, 3 kinds of rehabilitation plants are also nature
It is existing, it does not pollute and is endangered with other, be a kind of Eco-friendly arsenic cadmium lead zinc pollution administering method, be best at present square
Method.
Detailed description of the invention
Fig. 1 is using this technology processing arsenic in soil cadmium lead zinc pollution process comparison.
Specific embodiment
Below in conjunction with example, the invention will be further described:
Embodiment 1:
1. 100 grams of starch is added in 10 liters of bioreactors, 60 grams of coarse colza meals, 40 grams of dipotassium hydrogen phosphates, 30 grams
Potassium dihydrogen phosphate, 10 grams of calcium carbonate powders, 15 grams of ferrous sulfate, 10 grams of magnesium sulfate, 7000 milliliters of water start blender stirring,
Heating steam pipe is started, mixed solution is heated, lower 126 DEG C of 0.1Mpa are kept for 45 minutes, and sterilizing terminates, and is cooled to 30 DEG C of inoculations
The thermophilic hot rod bacterium of 0.5% (mass/volume), stirring, 50 revs/min of agitator speed, the lift-off of ventilatory capacity 0.5 gas/liter liquid/
Minute, constant temperature is mixed 24 hours, and fermentation ends put tank.Same method is distinguished in above-mentioned same liquid fermentation medium
It is inoculated with Thiobacillus thioxidans, arsenic oxidizing bacteria, Streptomyces glaucoviolaceus, the Dare Ford bacterium, children's set sacculus of 0.5% (mass/volume)
Bacterium is cultivated respectively, starts blender, 50 revs/min of agitator speed, ventilatory capacity 0.5 goes up to the air gas/liter liquid/minute, and constant temperature is mixed
Culture 24 hours is closed, fermentation ends put tank.
2. by above-mentioned 1, treated that 6 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, keeps viable bacteria and bud in filtrate
Spore content >=500,000,000/milliliter, wherein Dare Ford bacterium, children cover blastocyst viable bacteria and spore content >=100,000,000/milliliter, by 6 kinds of bacterium solutions
It is mixed into composite bacteria liquid preparation in equal volume.
3. the soil to be repaired of deep ploughing, by above-mentioned 2, treated that composite bacteria agent sprays agricultural land soil surface, uniformly spray per acre
200 kilograms of composite bacteria agent are applied, is poured water immediately, so that soil surface has ponding 10cm or more, and keeps soil surface dampness
20 days or more, then naturally dry soil moisture plants Sedum alfredii Hance, rich arsenic fern and rich arsenic penny cress.
4. in soil surface using spacing in the rows × line-spacing=15cm × 15cm plant Sedum alfredii Hance, rich arsenic fern and penny cress children
Seedling can be transplanted seeding row spacing, Sedum alfredii Hance and rich arsenic fern as the case may be with appropriate adjustment and penny cress interval is transplanted, is i.e. the southeast
Red-spotted stonecrop-richness arsenic fern-Sedum alfredii Hance-penny cress, plant finish, spray 200 kilograms of composite bacteria agents for the second time, and pour water immediately, make
Agricultural land soil surface layer is completely moist and by a small amount of ponding, maintain field 20 days moist or more, according to arsenic in soil pollution level, weight
It sprays microbial inoculum number 10 times, will pour water immediately after spraying microbial inoculum every time again, and keep soil surface completely wet, and keep 20
More than it, be proliferated in the soil so that hyperaccumulative plant is survived with microbial nutrition body in microbial inoculum and gemma, biological oxidation and/
Or reduction arsenic becomes the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide substance and arsenic hydride.
5. can gather in these plants after the Sedum alfredii Hance of plant, rich arsenic fern, penny cress yield positive results, deep plough immediately
Soil continues to transplant Sedum alfredii Hance-richness arsenic fern-Sedum alfredii Hance-penny cress according to above-mentioned planting patterns, and sprays composite bacteria agent,
It pours water immediately, and keeps different surface layers completely 20 days moist or more, can plant within 1 year 3 times, gather in 3 times, the harvested southeast
Red-spotted stonecrop, rich arsenic fern, penny cress etc. put together, and dry, and power transmission factory burns, or local centralized burning, ash collecting get up, periodically
The heavy metal cadmium lead zinc for sending fixed-end forces company to be uniformly processed in ashes.It, can be continuous kind according to the concrete condition of soil pollution
It plants 3 years, the heavy metal contaminants in soil can substantially be removed.
Embodiment 2:
1. 400 grams of starch is added in 10 liters of bioreactors, 100 grams of coarse colza meals, 90 grams of dipotassium hydrogen phosphates, 60 grams
Potassium dihydrogen phosphate, 60 grams of calcium carbonate powders, 50 grams of ferrous sulfate, 40 grams of magnesium sulfate, 7000 milliliters of water start blender stirring,
Heating steam pipe is started, mixed solution is heated, lower 126 DEG C of 0.1Mpa are kept for 45 minutes, and sterilizing terminates, and is cooled to 30 DEG C of inoculations
The thermophilic hot rod bacterium of 4% (mass/volume), stirring, 80 revs/min of agitator speed, the lift-off of ventilatory capacity 0.8 gas/liter liquid/point
Clock, constant temperature are mixed 36 hours, and fermentation ends put tank.Same method connects respectively in above-mentioned same liquid fermentation medium
Thiobacillus thioxidans, arsenic oxidizing bacteria, Streptomyces glaucoviolaceus, Dare Ford bacterium, the children set blastocyst of 4% (mass/volume) of kind, point
It does not cultivate, starts blender, 80 revs/min of agitator speed, ventilatory capacity 0.8 goes up to the air gas/liter liquid/minute, constant temperature mixing training
It supports 48 hours, fermentation ends put tank.
2. by above-mentioned 1, treated that 6 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, keeps viable bacteria and bud in filtrate
Spore content >=500,000,000/milliliter, wherein Dare Ford bacterium, children cover blastocyst viable bacteria and spore content >=100,000,000/milliliter, by 6 kinds of bacterium solutions
It is mixed into composite bacteria liquid preparation in equal volume.
3. the soil to be repaired of deep ploughing, by above-mentioned 2, treated that composite bacteria agent sprays agricultural land soil surface, uniformly spray per acre
Composite bacteria agent double centner is applied, is poured water immediately, so that soil surface has ponding 10cm or more, and keeps soil surface dampness
20 days or more, then naturally dry soil moisture plants Sedum alfredii Hance, rich arsenic fern and rich arsenic penny cress.
4. in soil surface using spacing in the rows × line-spacing=15cm × 15cm plant Sedum alfredii Hance, rich arsenic fern and penny cress children
Seedling can be transplanted seeding row spacing, Sedum alfredii Hance and rich arsenic fern as the case may be with appropriate adjustment and penny cress interval is transplanted, is i.e. the southeast
Red-spotted stonecrop-richness arsenic fern-Sedum alfredii Hance-penny cress, plant finish, spray double centner composite bacteria agent for the second time, and pour water immediately, make
Agricultural land soil surface layer is completely moist and by a small amount of ponding, maintain field 20 days moist or more, according to arsenic in soil pollution level, weight
It sprays microbial inoculum number 6 times, will pour water immediately after spraying microbial inoculum every time again, and keep soil surface completely wet, and keep 20
More than it, be proliferated in the soil so that hyperaccumulative plant is survived with microbial nutrition body in microbial inoculum and gemma, biological oxidation and/
Or reduction arsenic becomes the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide substance and arsenic hydride.
5. can gather in these plants after the Sedum alfredii Hance of plant, rich arsenic fern, penny cress yield positive results, deep plough immediately
Soil continues to transplant Sedum alfredii Hance-richness arsenic fern-Sedum alfredii Hance-penny cress according to above-mentioned planting patterns, and sprays composite bacteria agent,
It pours water immediately, and keeps different surface layers completely 20 days moist or more, can plant within 1 year 3 times, gather in 3 times, the harvested southeast
Red-spotted stonecrop, rich arsenic fern, penny cress etc. put together, and dry, and power transmission factory burns, or local centralized burning, ash collecting get up, periodically
Heavy metal cadmium lead zinc for sending fixed-end forces company to be uniformly processed in ashes etc..
Embodiment 3
1. 700 grams of starch is added in 10 liters of bioreactors, 300 grams of coarse colza meals, 130 grams of dipotassium hydrogen phosphates, 130
Gram potassium dihydrogen phosphate, 120 grams of calcium carbonate powders, 90 grams of ferrous sulfate, 80 grams of magnesium sulfate, 7000 milliliters of water start blender and stir
It mixes, starts heating steam pipe, heat mixed solution, lower 126 DEG C of 0.1Mpa are kept for 45 minutes, and sterilizing terminates, and is cooled to 30 DEG C and connects
The thermophilic hot rod bacterium of 8% (mass/volume) of kind, stirring, 120 revs/min of agitator speed, the lift-off of ventilatory capacity 1 gas/liter liquid/
Minute, constant temperature is mixed 48 hours, and fermentation ends put tank.Same method is distinguished in above-mentioned same liquid fermentation medium
It is inoculated with Thiobacillus thioxidans, arsenic oxidizing bacteria, Streptomyces glaucoviolaceus, Dare Ford bacterium, the children set blastocyst of 8% (mass/volume),
It cultivates respectively, starts blender, 50-120 revs/min of agitator speed, ventilatory capacity 1 goes up to the air gas/liter liquid/minute, and constant temperature is mixed
Culture 72 hours is closed, fermentation ends put tank.
2. by above-mentioned 1, treated that 6 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, keeps viable bacteria and bud in filtrate
Spore content >=500,000,000/milliliter, wherein Dare Ford bacterium, children cover blastocyst viable bacteria and spore content >=100,000,000/milliliter, by 6 kinds of bacterium solutions
It is mixed into composite bacteria liquid preparation in equal volume.
3. the soil to be repaired of deep ploughing, by above-mentioned 2, treated that composite bacteria agent sprays agricultural land soil surface, uniformly spray per acre
50 kilograms of composite bacteria agent are applied, is poured water immediately, so that soil surface has ponding 10cm or more, and keeps soil surface dampness 20
More than it, then naturally dry soil moisture plants Sedum alfredii Hance, rich arsenic fern and rich arsenic penny cress.
4. in soil surface using spacing in the rows × line-spacing=15cm × 15cm plant Sedum alfredii Hance, rich arsenic fern and penny cress children
Seedling can be transplanted seeding row spacing, Sedum alfredii Hance and rich arsenic fern as the case may be with appropriate adjustment and penny cress interval is transplanted, is i.e. the southeast
Red-spotted stonecrop-richness arsenic fern-Sedum alfredii Hance-penny cress, plant finish, and spray 50 kilograms of composite bacteria agents for the second time, and pour water immediately, so that
Agricultural land soil surface layer is completely moist and by a small amount of ponding, maintain field 20 days moist or more, according to arsenic in soil pollution level, repeat
It sprays microbial inoculum number 4 times, will pour water immediately after spraying microbial inoculum every time, and keep soil surface completely wet, and kept for 20 days
More than, be proliferated in the soil so that hyperaccumulative plant is survived with microbial nutrition body in microbial inoculum and gemma, biological oxidation and/or
Restoring arsenic becomes the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride.
5. can gather in these plants after the Sedum alfredii Hance of plant, rich arsenic fern, penny cress yield positive results, deep plough immediately
Soil continues to transplant Sedum alfredii Hance-richness arsenic fern-Sedum alfredii Hance-penny cress according to above-mentioned planting patterns, and sprays composite bacteria agent,
It pours water immediately, and keeps different surface layers completely 20 days moist or more, can plant within 1 year 2 times, gather in 2 times, the harvested southeast
Red-spotted stonecrop, rich arsenic fern, penny cress etc. put together, and dry, and power transmission factory burns, or local centralized burning, ash collecting get up, periodically
The heavy metal cadmium lead zinc for sending fixed-end forces company to be uniformly processed in ashes.
Application example of the invention:
By the technology of the present invention be used to pollute various heavy soil remediation using.
1, referring to embodiment 1, the soil containing refreshing cadmium lead zinc pollution is handled using this technology, is sprayed by continuous 8 times multiple
After combined bacteria agent, 3 plantations and harvesting Sedum alfredii Hance-richness arsenic fern-penny cress post-process 12 months, pair with not using this technology
It is compared according to soil, As is remained in soil and reduces 43%, Cd reduction 28%, Pb reduction 31%, 42% (Fig. 1) of Zn reduction；
2, referring to embodiment 2, the soil containing refreshing cadmium lead zinc pollution is handled using this technology, is sprayed by continuous 8 times multiple
After combined bacteria agent, 3 plantations and harvesting Sedum alfredii Hance-richness arsenic fern-penny cress post-process 12 months, pair with not using this technology
It is compared according to soil, As is remained in soil and reduces 45%, Cd reduction 34%, Pb reduction 36%, Zn reduction 45%.
3, referring to embodiment 3, the soil containing refreshing cadmium lead zinc pollution is handled using this technology, is sprayed by continuous 8 times multiple
After combined bacteria agent, 3 plantations and harvesting Sedum alfredii Hance-richness arsenic fern-penny cress post-process 12 months, pair with not using this technology
It is compared according to soil, As is remained in soil and reduces 51%, Cd reduction 38%, Pb reduction 38%, Zn reduction 46%.
Claims (7)
1. a kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application, it is characterised in that step are as follows:
A. 100-700 grams of starch, 60-300 grams of coarse colza meal, 40-130 grams of phosphoric acid hydrogen two are added in 10 liters of bioreactors
Potassium, 30-130 grams of potassium dihydrogen phosphate, 10-120 grams of calcium carbonate powder, 15-90 grams of ferrous sulfate, 10-80 grams of magnesium sulfate, 7000 millis
Water is risen, blender stirring is started, starts heating steam pipe, heats mixed solution, lower 126 DEG C of 0.1Mpa are kept for 45 minutes, sterilizing
Terminate, be cooled to the thermophilic hot rod bacterium of 30 DEG C of inoculations 0.5-8% (mass/volume), stirs, 50-120 revs/min of agitator speed
Clock, ventilatory capacity 0.5-1 go up to the air gas/liter liquid/minute, and constant temperature is mixed 24-48 hours, and fermentation ends put tank.Same side
Method is inoculated with the Thiobacillus thioxidans of 0.5-8% (mass/volume), arsenic oxidation carefully in above-mentioned same liquid fermentation medium respectively
Bacterium, Streptomyces glaucoviolaceus, Dare Ford bacterium, children set blastocyst, cultivate respectively, start blender, and 50-120 revs/min of agitator speed
Clock, ventilatory capacity 0.5-1 go up to the air gas/liter liquid/minute, and constant temperature is mixed 24-96 hours, and fermentation ends put tank.
B. by above-mentioned a, treated that 6 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, and viable bacteria and gemma in filtrate is kept to contain
>=5 hundred million/milliliters are measured, wherein Dare Ford bacterium, children set blastocyst viable bacteria and spore content >=100,000,000/milliliter, by bodies such as 6 kinds of bacterium solutions
Product is mixed into composite bacteria liquid preparation.
C. it deep ploughs soil to be repaired, by above-mentioned b, treated that composite bacteria agent sprays agricultural land soil surface, uniformly sprays per acre multiple
50-200 kilograms of combined bacteria agent, pours water immediately, so that soil surface has ponding 10cm or more, and keeps soil surface dampness 20
More than it, then naturally dry soil moisture plants Sedum alfredii Hance, rich arsenic fern and rich arsenic penny cress.
D. in soil surface using spacing in the rows × line-spacing=15cm × 15cm plant Sedum alfredii Hance, rich arsenic fern and penny cress seedling, root
According to concrete condition seeding row spacing can be transplanted with appropriate adjustment.Sedum alfredii Hance and rich arsenic fern and penny cress interval are transplanted, i.e. Sedum alfredii Hance-
Rich arsenic fern-Sedum alfredii Hance-penny cress, plant finish, and spray 50-200 kilograms of composite bacteria agent for the second time, and pour water immediately, so that
Agricultural land soil surface layer is completely moist and by a small amount of ponding, maintain field 20 days moist or more, according to arsenic in soil pollution level, repeat
It sprays microbial inoculum number 4-15 times, will pour water immediately after spraying microbial inoculum every time, and keep soil surface completely wet, and keep 20
More than it, be proliferated in the soil so that hyperaccumulative plant is survived with microbial nutrition body in microbial inoculum and gemma, biological oxidation and/
Or reduction arsenic becomes the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride.
E. after the Sedum alfredii Hance of plant, rich arsenic fern, penny cress yield positive results, these plants can be gathered in, soil of deep ploughing immediately,
Continue to transplant Sedum alfredii Hance-richness arsenic fern-Sedum alfredii Hance-penny cress according to above-mentioned planting patterns, and spray composite bacteria agent, fills immediately
Water, and keep different surface layers completely 20 days moist or more, it can plant 2-3 times within 1 year, gather in 2-3 times, harvested southeast scape
It, rich arsenic fern, penny cress etc. put together, dry, power transmission factory burns, or local centralized burning, ash collecting get up, periodically send
The heavy metal in ashes is uniformly processed in fixed-end forces company.According to the concrete condition of soil pollution, 1-10 can be continuously planted.
2. a kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application according to claim 1, feature
It is that the method is the starch that a. is added 100-700 grams in 10 liters of bioreactors, 60-300 grams of coarse colza meal, 40-130
Gram dipotassium hydrogen phosphate, 30-130 grams of potassium dihydrogen phosphate, 10-120 grams of calcium carbonate powder, 15-90 grams of ferrous sulfate, 10-80 grams of sulphur
Sour magnesium, 7000 milliliters of water start blender stirring, start heating steam pipe, heat mixed solution, the lower 126 DEG C of holdings of 0.1Mpa
45 minutes, sterilizing terminated, and was cooled to the thermophilic hot rod bacterium of 30 DEG C of inoculations 0.5-8% (mass/volume), stirring, agitator speed
50-120 revs/min, ventilatory capacity 0.5-1 goes up to the air gas/liter liquid/minute, and constant temperature is mixed 24-48 hours, and fermentation ends are put
Tank.Same method is inoculated with the sulfur oxide sulphur bar of 0.5-8% (mass/volume) in above-mentioned same liquid fermentation medium respectively
Bacterium, arsenic oxidizing bacteria, Streptomyces glaucoviolaceus, Dare Ford bacterium, children set blastocyst, cultivate respectively, start blender, agitator speed
50-120 revs/min, ventilatory capacity 0.5-1 goes up to the air gas/liter liquid/minute, and constant temperature is mixed 24-72 hours, and fermentation ends are put
Tank.
3. a kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application according to claim 1, feature
It is in the method, by above-mentioned a, treated that 6 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, keeps living in filtrate
Bacterium and spore content >=500,000,000/milliliter, wherein Dare Ford bacterium, children cover blastocyst viable bacteria and spore content >=100,000,000/milliliter, by 6
Kind bacterium solution is mixed into composite bacteria liquid preparation in equal volume.
4. a kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application according to claim 1, feature
It is the method, soil to be repaired of deep ploughing, treated that composite bacteria agent sprays agricultural land soil surface by above-mentioned b, per per mu
It is even to spray 50-200 kilograms of composite bacteria agent, it pours water immediately, so that soil surface has ponding 10cm or more, and keeps soil surface damp
Wet condition 20 days or more, then naturally dry soil moisture plants Sedum alfredii Hance, rich arsenic fern and rich arsenic penny cress.
5. a kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application according to claim 1, feature
It is the method, in soil surface using spacing in the rows × line-spacing=15cm × 15cm plant Sedum alfredii Hance, rich arsenic fern and penny cress
Seedling can be transplanted seeding row spacing, Sedum alfredii Hance and rich arsenic fern as the case may be with appropriate adjustment and penny cress interval is transplanted, i.e., eastern
Southern red-spotted stonecrop-richness arsenic fern-Sedum alfredii Hance-penny cress, plant finish, and spray 50-200 kilograms of composite bacteria agent for the second time, and fill immediately
Water, so that agricultural land soil surface layer is completely moist and by a small amount of ponding, maintain field 20 days moist or more, according to soil arsenic pollution journey
Degree, repeat spraying microbial inoculum number 4-10 times, will pour water immediately after spraying microbial inoculum every time, and keep soil surface completely wet,
And kept for 20 days or more, it is proliferated in the soil so that hyperaccumulative plant is survived with microbial nutrition body in microbial inoculum and gemma, is raw
Object oxidation and/or reduction arsenic become the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride.
6. a kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application according to claim 1, feature
It is the method, after the Sedum alfredii Hance of plant, rich arsenic fern, penny cress yield positive results, these plants can be gathered in, it is deep immediately
Soil is ploughed, continues to transplant Sedum alfredii Hance-richness arsenic fern-Sedum alfredii Hance-penny cress according to above-mentioned planting patterns, and spray compound bacteria
Agent is poured water immediately, and keeps different surface layers completely 20 days moist or more, can be planted 2-3 times within 1 year, be gathered in 2-3 times, after harvesting
Sedum alfredii Hance, rich arsenic fern, penny cress etc. put together, dry, power transmission factory burns, or local centralized burning, ash collecting rise
Come, periodically send fixed-end forces company that the heavy metal in ashes is uniformly processed.It, can be continuous kind according to the concrete condition of soil pollution
It plants several years.
7. any a kind of improvement arsenic in soil zinc cadmium control of Pb pollution method of claim 1~6 and its in farmland arsenic zinc cadmium lead
Contaminated soil, refuse landfill, municipal sludge, gas station, stope, electroplate factory, electrolysis plant, metallurgical works, contaminated sites
The application that soil pollution arsenic zinc cadmium lead dye is repaired.
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Application publication date: 20181207 |