CN108587947A - One plant of phosphorus-solubilizing bacteria and its application with DEHP degradation bacterias composite bacteria agent and in soil improvement - Google Patents
One plant of phosphorus-solubilizing bacteria and its application with DEHP degradation bacterias composite bacteria agent and in soil improvement Download PDFInfo
- Publication number
- CN108587947A CN108587947A CN201810316362.XA CN201810316362A CN108587947A CN 108587947 A CN108587947 A CN 108587947A CN 201810316362 A CN201810316362 A CN 201810316362A CN 108587947 A CN108587947 A CN 108587947A
- Authority
- CN
- China
- Prior art keywords
- composite bacteria
- ylyp1
- bacteria agent
- soil
- dehp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/11—Bacillus megaterium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- A—HUMAN NECESSITIES
- A62—LIFE-SAVING; FIRE-FIGHTING
- A62D—CHEMICAL MEANS FOR EXTINGUISHING FIRES OR FOR COMBATING OR PROTECTING AGAINST HARMFUL CHEMICAL AGENTS; CHEMICAL MATERIALS FOR USE IN BREATHING APPARATUS
- A62D3/00—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances
- A62D3/02—Processes for making harmful chemical substances harmless or less harmful, by effecting a chemical change in the substances by biological methods, i.e. processes using enzymes or microorganisms
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2101/00—Agricultural use
Abstract
The present invention discloses one plant of phosphorus-solubilizing bacteria and its application with DEHP degradation bacterias composite bacteria agent and in soil improvement.The bacterial strain is Bacillus megaterium YLYP1, and Guangdong Province's Culture Collection, deposit number were preserved on 06 21st, 2017：GDMCC NO：60204.The present invention also provides a kind of composite bacteria agents, including YLYP1 and Rhodococcus pyridinivorans XB.The composite bacteria agent concertedness is preferable, its phosphate solubilization and DEHP degradation capabilities are significantly improved, and cultural method is simple and easy to do, breeding is rapid, adaptable, and the situation of soil phosphorus shortage can be both effectively improved using YLYP1 and XB, it also can remove DEHP organic pollutions in soil simultaneously, to cultivating and being promoted soil ecology fertility, ensure that agricultural product quality and safety, realization are repaired in production and be of great significance.
Description
Technical field
The invention belongs to microbial engineering fields, and in particular to one plant of phosphorus-solubilizing bacteria and its with DEHP degradation bacteria compound bacterias
Agent and the application in soil improvement.
Background technology
Phosphorus (P) is one of macronutrient necessary to growth and development of plants, participates in a variety of physiological metabolism activities of plant
And morphogenesis, decide the yield and quality of crops.Phosphorus element needed for plant is mainly derived from soil.Due to the phosphorus in soil
Element exists usually in the form of difficulty soluble salt, and most of agricultural land soil phosphorus elements is caused to lack.There are about 70% or more arable lands to lack in China
Weary phosphorus element.In recent years, with the continuous improvement of crop yield, the amount of application of phosphate fertilizer also continues to increase, and applies the phosphorus in farmland
Fertilizer is but easily by Ca2+、Fe3+、Al3+It is deposited Deng cationic in conjunction with insoluble phosphate is formed, is fixed on soil, so as to cause
Crop only has 3.7~32.4% to this season utilization rate of phosphate fertilizer.Agriculture production cost is not only largely improved using phosphate fertilizer, can also be led
Soil hardening and fertility is caused to decline and cause a series of environmental problems such as water pollution and soil pollution.Therefore, pass through Soluble phosphorus
Bacterium discharges by the phosphorus element of soil fixing, reduces the usage amount of phosphate fertilizer, is one of the utilization rate effective means for improving phosphorus, is to cope with me
The important measure of state's chemical fertilizer zero growth policy, is of great significance for China's agricultural sustainable development.
On the other hand since Industrial " three Waste " largely discharges and the unreasonable of the agricultural material products such as chemical fertilizer, pesticide, agricultural film makes
With, cause the organic pollutions such as agricultural land soil phthalic acid ester (PAEs), polycyclic aromatic hydrocarbon (PAHs) pollute.In contaminated soil
The organic pollutions such as PAEs seriously affect agricultural product quality and safety by crops uptake and accumulation.The organic pollutions such as PAEs belong to
Environment incretion interferent can have Reproductive and developmental toxicity, hepatotoxicity and " three cause " effect (carcinogenic, cause in people's cylinder accumulation
Abnormal, mutagenesis), agricultural product of resident's daily consumption containing organic pollutions such as PAEs cause long-term low dose to expose, and seriously jeopardize
Health.
Therefore, for China's agricultural land soil phosphorus element lack and PAEs pollution present situation, research and development have Soluble phosphorus and
The composite bacteria agent of PAEs degradation functions is of great significance.
Invention content
In order to overcome the disadvantages and deficiencies of the prior art, the purpose of the present invention is to provide a kind of phosphorus-solubilizing bacterias.
Another object of the present invention is to provide a kind of composite bacteria agents.
It is still another object of the present invention to provide the applications of above-mentioned phosphorus-solubilizing bacteria, composite bacteria agent.
Phosphorus-solubilizing bacteria and its composite bacteria agent provided by the invention can not only solve the problems, such as that soil phosphorus lacked, but also can reduce soil
Earth DEHP pollutions, it is ensured that agricultural product quality and safety.
The purpose of the invention is achieved by the following technical solution：
The present invention provides one plant of phosphorus-solubilizing bacteria, is named as bacillus megaterium (Bacillus megaterium) YLYP1, is
It is isolated and purified from the activated sludge of sewage treatment plant.
The phosphorus-solubilizing bacteria is Bacillus megaterium YLYP1, preservation information：Depositary institution：Guangdong Province is micro-
Biological inoculum collection (GDMCC), preservation date：On 06 21st, 2017, preservation address：Xianlie Middle Road, Guangzhou City 100
5 building, the building of compound the 59th Guangdong Microbes Inst, deposit number：GDMCC NO：60204.
Bacillus megaterium (Bacillus megaterium) the YLYP1 thalline cultural characteristic and morphological feature：Thalline
For gram-positive bacteria；There is gemma, is given birth in gemma or end life partially, gemma are oval；Observed under electron microscope is in elongated rod shape,
1.9~2.2 μm of thalline average length, mean breadth 0.7~0.9 μm (Fig. 1)；It is cultivated for 24 hours for 30 DEG C on LB plating mediums, bacterium
It is in milk yellow to fall, the flat protuberance of bacterium colony, neat in edge, and surface is smooth opaque (Fig. 2)；The Physiology and biochemistry of the bacterial strain YLYP1
The results are shown in Table 1 for identification experiment.
The present invention also provides applications of the above-mentioned Bacillus megaterium YLYP1 in dissolving difficultly-soluble phosphates.
The difficultly-soluble phosphates are calcium phosphate, aluminum phosphate or ferric phosphate.
Preferably, the difficultly-soluble phosphates are calcium phosphate.
The Bacillus megaterium YLYP1 are in NBRIP culture mediums, and dissolving insoluble phosphate is to solvable
Property phosphorus content is up to 95.3 ± 3.2mg/L~405.0 ± 3.7mg/L.
The present invention also provides a kind of composite bacteria agents, including Bacillus megaterium YLYP1 and Rhodococcus
pyridinivorans XB。
Thermophilic pyridine Rhodococcus sp (Rhodococcus pyridinivorans) XB of this seminar early-stage study separation screening
Diisooctyl phthalate in soil (DEHP) can be effectively reduced, and reduces uptake and accumulation of the corn to DEHP.The strain
Preservation information：Depositary institution：Guangdong Province's Culture Collection (GDMCC), preservation date：On 09 28th, 2016,
Preservation address：5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100 Guangdong Microbes Inst, deposit number：GDMCC
NO：60054.
The Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are made dense
Degree is 108~1010The bacteria suspension of cfu/mL；
The Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are according to body
Product ratio 2~5：1~3 is mixed；It is preferred that according to volume ratio 2：1~3 is mixed；More preferably according to volume ratio 2：1 is mixed
It closes.
The preparation method of the composite bacteria agent, includes the following steps：
(1) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated with respectively
In LB liquid medium, 28~30 DEG C of 12~18h of activation；
(2) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, and it is 10 to be cultivated respectively to bacterial population8
~1010cfu/mL；
(3) by 2 kinds of single bacteriums according to 2~5：1~3 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
Application of the composite bacteria agent in Soluble phosphorus and/or degradation DEHP.
The composite bacteria agent is in NBRIP culture mediums, and titanium pigment content is up to 482.5~570.3mg/L.
The composite bacteria agent is improved the degradation capability of DEHP in the MSM culture mediums containing DEHP, can incite somebody to action
DEHP substantially completely degrades.
The results show that in Bacillus megaterium YLYP1 culture solutions titanium pigment content be 405.0mg/L~
Titanium pigment content 15.7mg/L~20.2mg/ in 436.2mg/L, Rhodococcus pyridinivorans XB culture solutions
L, and titanium pigment content 482.5mg/L~570.3mg/L in composite bacteria agent culture solution.Illustrate that composite bacteria agent phosphate solubilization obtains
It is obviously improved, reaches 1+1>2 microbial inoculum compounding effects.
DEHP degradation rates measure：DEHP is not in the MSM culture mediums containing DEHP by Bacillus megaterium YLYP1
It degrades, Rhodococcus pyridinivorans XB are in the MSM culture mediums containing DEHP, to the degradation rate of DEHP
Be 80.5%~91.9%, and composite bacteria agent is in the MSM culture mediums containing DEHP, to DEHP degradation rates be 90.5%~
100%.Therefore, composite bacteria agent is improved the degradation capability of DEHP.
Application of the composite bacteria agent in improveing soil quality.
Preferably, composite bacteria agent is connect and is added in soil, and stirred evenly, available phosphorus content in soil can be made to increase,
And the DEHP in soil of degrading simultaneously.
600g soil is added in the beaker of 500mL, masking foil shading treatment is used in combination, adds bacterial strain respectively into soil
YLYP1, XB and compounding each 60mL of microbial inoculum, mix well.Soil moisture content is adjusted to field capacity 30%, 30 DEG C of constant temperature
It is protected from light culture 10d, is control not to be inoculated with any microbial inoculum soil, three repetitions of each processing setting.Measure different disposal soil
Middle available phosphorus content.The results show that available phosphorus content 39.8mg/kg in control soil, inoculation single bacterium Bacillus
59.5~68.1mg/kg of available phosphorus content in megaterium YLYP1 soil, inoculation single bacterium Rhodococcus
41.7~45.2mg/kg of available phosphorus content in pyridinivorans XB soil, inoculation compound available phosphorus content in microbial inoculum soil
For 76.2~87.2mg/kg.
A concentration of 20mg/kg of DEHP are set.600g soil is added in the beaker of 500mL, DEHP is added, stirs evenly,
It is used in combination masking foil shading treatment, after aging for a week, adds bacterial strain YLYP1, XB, composite bacteria agent 60mL respectively into soil, stir
Uniformly.Soil moisture content is adjusted to 30%, 30 DEG C of constant temperature incubation 15d of field capacity, measures the residual quantity of DEHP in soil,
Not add the soil of any microbial inoculum as blank control.3 repetitions are arranged in above-mentioned each processing.The results show that in blank control
Degradation of DEHP in soil rate 27.1% is inoculated with single bacterium Bacillus megaterium YLYP1 degradation of DEHP in soil rates
32.2%~36.5%, inoculation single bacterium Rhodococcus pyridinivorans XB degradation of DEHP in soil rate 53.9%~
65.3%, the degradation rate for adding DEHP in the soil of composite bacteria agent is respectively 70.3%~93.7%.
The present invention has the following advantages and effects with respect to the prior art：
Multi-functional composite bacteria agent concertedness disclosed by the invention is preferable, the phosphate solubilization and DEHP degradation capabilities of composite bacteria agent
It is significantly improved, the composite bacteria agent cultural method is simple and easy to do, and breeding is rapid, adaptable, has good application
Potentiality utilize phosphorus-solubilizing bacteria Bacillus megaterium YLYP1 and DEHP degradation bacterias Rhodococcus
Pyridinivorans XB can both be effectively improved the situation of soil phosphorus shortage, while can also remove DEHP in soil and have
Machine pollutant ensures agricultural product quality and safety, realizes and repaired with important in production to cultivating and being promoted soil ecology fertility
Meaning.
Description of the drawings
Fig. 1 is bacillus megaterium (Bacillus megaterium) YLYP1 cellular morphologies.
Fig. 2 is bacillus megaterium (Bacillus megaterium) YLYP1 colonial morphologies.
Fig. 3 is bacillus megaterium (Bacillus megaterium) YLYP1 phylogenetic evolution trees.
Specific implementation mode
Present invention will now be described in further detail with reference to the embodiments and the accompanying drawings, but embodiments of the present invention are unlimited
In this.
The preservation information of bacterial strain Rhodococcus pyridinivorans XB used in embodiment：Depositary institution：Extensively
East saves Culture Collection (GDMCC), preservation date：On 09 28th, 2016, preservation address：Xianlie Middle Road, Guangzhou City
No. 100 5 building, the buildings of compound the 59th Guangdong Microbes Inst, deposit number：GDMCC NO：60054.
Minimal medium (MSM, gL-1)：K2HPO45.8, KH2PO44.5, (NH4)2SO42.0, MgCl20.16,
CaCl20.02, Na2MoO4·2H2O 0.0024, FeCl30.0018, MnCl2·2H2O0.0015, pH 7.5.It is cultivated in MSM
It is 400mg L that DEHP initial concentrations are added in base-1, as sole carbon source.
Embodiment 1
The screening of phosphorus-solubilizing bacteria：
(1) separation method of the bacterial strain is plate screening model：The activated sludge for acquiring sewage treatment plant is sterile in 50mL
In centrifuge tube, it is placed in clean ice chest.1g activated sludge is weighed to be dissolved into the 50mL centrifuge tubes equipped with 40mL sterile waters,
Sample gradient dilution is made 10 by the oscillation of 130rpm oscillators at uniform, with 0.9% sterile saline-1~10-5Solution.
(2) culture medium：
Meng Jinna (PVK) fluid nutrient medium：Calcium phosphate 5.0g, sucrose 10.0g, ammonium sulfate 0.5g, sodium chloride 0.1g, seven water
Close magnesium sulfate 0.1g, potassium chloride 0.2g, manganese sulfate 0.03g, yeast extract 0.5g, distilled water 1000mL, pH 7.0~7.2.PVK is solid
Body culture medium then adds agar 15g.
Phosphate growth (NBRIP) culture medium：Glucose 10.0g, calcium phosphate 5.0g, Magnesium dichloride hexahydrate 5.0g, seven water
Close magnesium sulfate 0.25g, potassium chloride 0.2g, ammonium sulfate 0.1g, distilled water 1000mL, pH 7.0.
LB liquid medium：Yeast powder 5.0g, peptone 10.0g, sodium chloride 10.0g, distilled water 1000mL, pH 7.0.
LB solid mediums then add 15g agar.
All cultures are based on 121 DEG C, and sterilize 20min.
(3) phosphorus-solubilizing bacteria screens：The dilution 0.1mL for drawing different extension rates respectively, is spread evenly across PVK solid cultures
On base, be placed in 30 DEG C and be inverted culture 5 days, select grow fine, the significant bacterium colony of phosphorus decomposing circle, it is solid to be forwarded to PVK again with method of scoring
It on body culture medium, repeats 2~3 times, finally obtains one plant of stronger phosphorus-solubilizing bacteria of phosphate solubilization, number is YLYP1.It is transferred
To 4 DEG C of LB culture mediums test tube slant Storage in refrigerator, it to be used for subsequent experimental.
Embodiment 2
The YLYP1 identifications of Soluble phosphorus bacterial strain：
Morphological feature and the detection foundation and method of Physiology and biochemistry are Bacteria Identification program MS (i)/C005-C01.Form is reflected
It is fixed：The bacterial strain is gram-positive bacterium, there is a gemma, and raw in gemma or end life partially, gemma are oval；Under electron microscope
Elongated rod shape, 1.9~2.2 μm of thalline average length, mean breadth 0.7~0.9 μm (Fig. 1).30 DEG C of trainings on LB solid mediums
It supports for 24 hours, bacterium colony is in milk yellow, and the flat protuberance of bacterium colony, surface is smooth opaque, neat in edge (Fig. 2).
Physiological and biochemical property is identified：Bio-chemical characteristics the results are shown in Table 1.
The physiological and biochemical property of 1 Bacillus megateriumYLYP1 of table
| Physiological and biochemical test | As a result | Physiological and biochemical test | As a result |
| Catalase | + | D- glucose sugar ferments | - |
| Oxidizing ferment | - | Trehalose ferments | - |
| Anaerobic growth | - | Mannose ferments | - |
| V-P is measured | - | Maltose ferments | - |
| Nitrate reduction | - | Rhamnose ferments | - |
| Starch Hydrolysis | + | Movement | + |
| Gelatin liquefaction | + | 2%NaCl growth experiments | + |
| Phenylalanine deshydroxy enzyme | - | 5%NaCl growth experiments | + |
| Citrate utilizes | - | 7%NaCl growth experiments | - |
| Lecithinase | - | 10%NaCl growth experiments | - |
| Indoles | - | PH4 cultivates basal growth | - |
| Casein hydrolyzes | + | PH6 cultivates basal growth | + |
| Tyrosine hydrolysis | - | PH10 cultivates basal growth | + |
| 5 DEG C of growths | - | PH11 cultivates basal growth | - |
| 10 DEG C of growths | - | Urase | + |
| 20 DEG C of growths | + | Malonate utilizes | - |
| 30 DEG C of growths | + | Hydrogen sulfide production test | - |
| 40 DEG C of growths | + | Ornithine deshydroxy enzyme | - |
| 42 DEG C of growths | - | Arginine deshydroxy enzyme | - |
| Arginine dihydrolase | - |
+：It is positive；-：It is negative
Molecular biology identification：The bacterium total DNA is extracted using bacteria total DNA extracts kit, using bacterial 16 S rDNA
Universal primer F27 and R1492 carries out PCR amplification and sequencing (George et al., 2013), sequence such as SEQ to the bacterium genome
ID NO：Shown in 1.The 16S rDNA sequences reported in sequencing result and GenBank are subjected to sequence analysis, and are
System development phylogenetic analysis, 16S rDNA sequences and the Bacillus megaterium B2 of bacterial strain YLYP1
(KT307979.1) homology is higher, and for homology up to 99%, the phylogenetic evolution tree of structure sees Fig. 3.Therefore, according to the bacterium
Morphological feature, physiological and biochemical property and the phylogenetic analysis of strain, bacillus megaterium (Bacillus is accredited as by the bacterial strain
megaterium).Strain is named as Bacillus megaterium YLYP1.
The preservation information of the Bacillus megaterium YLYP1：Depositary institution：Guangdong Province microorganism fungus kind protects
Tibetan center (GDMCC), preservation date：On 06 21st, 2017, preservation address：No. 59 building 5 of compound of Xianlie Middle Road, Guangzhou City 100
Building Guangdong Microbes Inst, deposit number：GDMCC NO：60204.
Embodiment 3
Bacterial strain Bacillus megaterium YLYP1 phosphate solubilizations measure：
(1) preparation of bacteria suspension：It is preserved in the YLYP1 strains of test tube slant on a small quantity in 50mL LB liquid with oese picking
In body culture medium, in 30 DEG C, 150rpm, 12h is cultivated, takes 40mL culture solutions in 50mL centrifuge tubes, 4 DEG C, 3500rpm centrifugations
3min collects thalline, and with 0.9% sterile saline washing thalline 3 times, it is 10 to be used in combination physiological saline to prepare strain density8cfu/
The bacteria suspension of mL.
(2) phosphate pre-processes：20.0g analysis pure phosphoric acids aluminium, ferric phosphate or calcium phosphate are weighed in large beaker, it is super with 2L
Pure water ultrasonic dissolution discards upper layer turbid solution every 30min, retains aluminum phosphate, ferric phosphate or the calcium phosphate of beaker bottom indissoluble,
Supersound washing is repeated 2~3 times to upper solution clarification, and collects the aluminum phosphate, ferric phosphate or calcium phosphate for being deposited in beaker bottom,
60 DEG C of baking oven drying are placed in, after milling, is fitted into brown reagent bottle, is placed in spare in drier.
(3) NBRIP culture mediums are prepared：
With indissoluble aluminum phosphate, ferric phosphate or calcium phosphate be unique phosphorus source prepare culture medium, with NaOH solution adjust pH value to
7.0, sterilize 20min in 121 DEG C.NBRIP culture medium prescriptions are with reference to shown in 1 step of embodiment (2).
(4) it is inoculated with and cultivates：By the above-mentioned bacteria suspension (10 prepared8Cfu/mL) according to 4% (V of cultivating system：V)
Inoculum concentration is inoculated into NBRIP culture mediums.In 30 DEG C, 130rpm cultivates 5d.
(5) measurement of soluble phosphate：Cultivate 5d after, take culture solution 2mL in centrifuge tube, 4 DEG C, 3500rpm centrifugation
10min takes 0.2mL supernatants to be diluted to 10mL, and soluble phosphoric acid salt content (HJ 704- are measured with molybdenum-antimony anti-spectrophotometric method
Measurement sodium bicarbonate extraction-molybdenum-antimony anti-spectrophotometric method of 2014 soil available phosphorus).Made with the bacteria suspension of 121 DEG C of sterilizing 20min
For control.In NBRIP culture mediums, dissolving phosphoric acid aluminium obtains titanium pigment content and reaches 95.3 ± 3.2mg/L, dissolving phosphoric acid iron,
Obtain titanium pigment content and reach 130.4 ± 5.6mg/L, dissolving slightly solubility calcium phosphate discharge titanium pigment up to 405.0 ±
3.7mg/L.In order to exclude influence of the autoclave sterilization to soluble phosphoric acid salt content in culture solution, before we determine sterilizing
The phosphatic content of capacitive afterwards in culture solution, the results showed that, high pressure sterilization does not influence soluble phosphoric acid salt content.
Embodiment 4
Composite bacteria agent phosphate solubilization is analyzed：
(1) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated with respectively
In LB liquid medium, 30 DEG C of activation 12h；
(2) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, are cultivated respectively to bacterial population and are
109cfu/mL；
(3) by 2 kinds of single bacteriums according to 2：1 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
(4) by etc. thalline quantities composite bacteria agent, Bacillus megaterium YLYP1 and Rhodococcus
Pyridinivorans XB are inoculated into respectively in NBRIP culture mediums (unique phosphorus source uses calcium phosphate), and in 30 DEG C, 130rpm is trained
It supports 5 days, 3 repetitions of each processing setting.
(5) measurement of soluble phosphate：After culture 5 days, take culture solution 2mL in centrifuge tube, 4 DEG C, 3500rpm is centrifuged
10min takes 0.2mL supernatants to be diluted to 10mL, and the content of soluble phosphate is measured with molybdenum-antimony anti-spectrophotometric method.As a result it shows
Show, titanium pigment content is 413.2mg/L, Rhodococcus in Bacillus megaterium YLYP1 culture solutions
Titanium pigment content 18.2mg/L in pyridinivorans XB culture solutions, and titanium pigment content in composite bacteria agent culture solution
529.5mg/L illustrates that composite bacteria agent phosphate solubilization is significantly improved, reaches 1+1>2 microbial inoculum compounding effects.
Embodiment 5
Composite bacteria agent phosphate solubilization is analyzed：
(1) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated with respectively
In LB liquid medium, 28 DEG C of activation 12h；
(2) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, are cultivated respectively to bacterial population and are
108cfu/mL；
(3) by 2 kinds of single bacteriums according to 2：3 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
(4) by etc. thalline quantities composite bacteria agent, Bacillus megaterium YLYP1 and Rhodococcus
Pyridinivorans XB are inoculated into respectively in NBRIP culture mediums (unique phosphorus source uses calcium phosphate), and in 30 DEG C, 130rpm is trained
It supports 5 days, 3 repetitions of each processing setting.
(5) measurement of soluble phosphate：After culture 5 days, take culture solution 2mL in centrifuge tube, 4 DEG C, 3500rpm is centrifuged
10min takes 0.2mL supernatants to be diluted to 10mL, and the content of soluble phosphate is measured with molybdenum-antimony anti-spectrophotometric method.As a result it shows
Show, titanium pigment content is 405.0mg/L, Rhodococcus in Bacillus megaterium YLYP1 culture solutions
Titanium pigment content 15.7mg/L in pyridinivorans XB culture solutions, titanium pigment content in composite bacteria agent culture solution
482.5mg/L illustrates that composite bacteria agent phosphate solubilization is significantly improved, reaches 1+1>2 microbial inoculum compounding effects.
Embodiment 6
Composite bacteria agent phosphate solubilization is analyzed：
(1) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated with respectively
In LB liquid medium, 30 DEG C of activation 18h；
(2) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, are cultivated respectively to bacterial population and are
1010cfu/mL；
(3) by 2 kinds of single bacteriums according to 2：2 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
(4) by etc. thalline quantities composite bacteria agent, Bacillus megaterium YLYP1 and Rhodococcus
Pyridinivorans XB are inoculated into respectively in NBRIP culture mediums (unique phosphorus source uses calcium phosphate), and in 30 DEG C, 130rpm is trained
It supports 5 days, 3 repetitions of each processing setting.
(5) measurement of soluble phosphate：Culture takes culture solution 2mL in centrifuge tube after 5 days, 4 DEG C, 3500rpm is centrifuged
10min takes 0.2mL supernatants to be diluted to 10mL, and the content of soluble phosphate is measured with molybdenum-antimony anti-spectrophotometric method.As a result it shows
Show, titanium pigment content is 436.2mg/L, Rhodococcus in Bacillus megaterium YLYP1 culture solutions
Titanium pigment content 20.2mg/L in pyridinivorans XB culture solutions, and titanium pigment content in composite bacteria agent culture solution
570.3mg/L illustrates that composite bacteria agent phosphate solubilization is significantly improved, reaches 1+1>2 microbial inoculum compounding effects.
Embodiment 7
The capability analysis of composite bacteria agent degradation DEHP：
(1) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated with respectively
In LB liquid medium, 28 DEG C of activation 12h；
(2) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, are cultivated respectively to bacterial population and are
108cfu/mL；
(3) by 2 kinds of single bacteriums according to 2：1 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
(4) by etc. thalline quantities Bacillus megaterium YLYP1 and Rhodococcus
Pyridinivorans XB and composite bacteria agent are respectively connected to using DEHP as in the MSM culture mediums of sole carbon source, in 30 DEG C,
130rpm is cultivated 3 days, while not connect bacterium culture medium as blank control, 3 repetitions are arranged in each processing.
(5) DEHP does not degrade in Bacillus megaterium YLYP1 culture solutions, Rhodococcus
Pyridinivorans XB are 80.5% to the degradation rate of DEHP, and composite bacteria agent is 90.5% to DEHP degradation rates.Therefore,
Composite bacteria agent is improved the degradation capability of DEHP.
Embodiment 8
The capability analysis of composite bacteria agent degradation DEHP：
(1) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated with respectively
In LB liquid medium, 18h is activated at 30 DEG C；
(2) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, are cultivated respectively to bacterial population and are
1010cfu/mL；
(3) by 2 kinds of single bacteriums according to 2：3 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
(4) by etc. thalline quantities single bacterium Bacillus megaterium YLYP1 and Rhodococcus
Pyridinivorans XB and composite bacteria agent are respectively connected to using DEHP as in the MSM culture mediums of sole carbon source, in 30 DEG C,
It is cultivated 3 days in the sterile shaking table of 150rpm, while not connect bacterium culture medium as blank control, 3 repetitions are arranged in each processing.
(5) DEHP does not degrade in Bacillus megaterium YLYP1 culture solutions, Rhodococcus
Pyridinivorans XB are 91.9% to the degradation rate of DEHP, and composite bacteria agent is degradable by DEHP.Therefore, compound bacteria
Agent is improved the degradation capability of DEHP.
Embodiment 9
Composite bacteria agent is applied to phosphorus element and lacks soil improvement：
(1) for trying soil：Farmland rice soil, pH 5.62, organic matter 17.40g/kg, total nitrogen 0.79g/kg, total phosphorus
0.66g/kg crosses 60 mesh after air-drying.
(2) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated with respectively
In LB liquid medium, 30 DEG C of activation 18h；
(3) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, are cultivated respectively to bacterial population and are
108cfu/mL；
(4) by 2 kinds of single bacteriums according to 2：1 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
(5) available phosphorus content measures in soil：
600g soil is added in the beaker of 500mL, masking foil shading treatment is used in combination, adds bacterial strain respectively into soil
YLYP1, XB, composite bacteria agent 60mL, mix well.Soil moisture content is adjusted to the 30% of field capacity, is trained at 30 DEG C
10d is supported, the soil not to be inoculated with any microbial inoculum is control, three repetitions of each processing setting.Measuring has in different disposal soil
Phosphorus content is imitated, the results show that available phosphorus content 39.8mg/kg in control, inoculation single bacterium Bacillus megaterium YLYP1
Available phosphorus content 59.5mg/kg in soil is inoculated with available phosphorus in single bacterium Rhodococcus pyridinivorans XB soil and contains
41.7mg/kg is measured, available phosphorus content is 76.2mg/kg in inoculating compound bacterium agent soil.
Embodiment 10
Composite bacteria agent is applied to phosphorus element and lacks soil improvement：
(1) for trying soil：Farmland rice soil, pH 5.62, organic matter 17.40g/kg, total nitrogen 0.79g/kg, total phosphorus
0.66g/kg crosses 60 mesh after air-drying.
(2) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated with respectively
In LB liquid medium, 30 DEG C of activation 18h；
(3) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, are cultivated respectively to bacterial population and are
1010cfu/mL；
(4) by 2 kinds of single bacteriums according to 2：1 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
(5) available phosphorus content measures in soil：
600g soil is added in the beaker of 500mL, masking foil shading treatment is used in combination, adds bacterial strain respectively into soil
YLYP1, XB, composite bacteria agent 60mL, mix well.Soil moisture content is adjusted to the 30% of field capacity, is trained at 30 DEG C
10d is supported, the soil not to be inoculated with any microbial inoculum is control, three repetitions of each processing setting.Measuring has in different disposal soil
Phosphorus content is imitated, the results show that available phosphorus content 39.8mg/kg in control, inoculation single bacterium Bacillus megaterium YLYP1
Available phosphorus content 68.1mg/kg in soil is inoculated with available phosphorus in single bacterium Rhodococcus pyridinivorans XB soil and contains
45.2mg/kg is measured, available phosphorus content is 87.2mg/kg in inoculating compound bacterium agent soil.
Embodiment 11
Composite bacteria agent is to DEHP contaminated soil remediation applications：
(1) for trying soil：Farmland rice soil, pH 5.62, organic matter 17.40g/kg, total nitrogen 0.79g/kg, total phosphorus
0.66g/kg crosses 60 mesh after air-drying.
(2) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated with respectively
In LB liquid medium, 30 DEG C of activation 18h；
(3) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, are cultivated respectively to bacterial population and are
108cfu/mL；
(4) by 2 kinds of single bacteriums according to 2：3 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
(5) composite bacteria agent repairs DEHP contaminated soils
Setting DEHP pollution concentrations are 20mg/kg.600g soil is added in the beaker of 500mL, DEHP is added, stirring is equal
It is even, it is used in combination masking foil shading treatment, after aging for a week, adds bacterial strain YLYP1, XB, composite bacteria agent 60mL respectively into soil, stir
It mixes uniformly.Soil moisture content is adjusted to the 30% of field capacity, 30 DEG C of constant temperature incubation 15d, not add any microbial inoculum
Soil is blank control, 3 repetitions of above-mentioned each processing setting, the residual quantity of DEHP in measurement.The results show that in blank control
Degradation of DEHP in soil rate 27.1% is inoculated with single bacterium Bacillus megaterium YLYP1 degradation of DEHP in soil rates
32.2%, inoculation single bacterium Rhodococcus pyridinivorans XB degradation of DEHP in soil rate 53.9% adds compound bacteria
The degradation rate of DEHP is respectively 70.3% in the soil of agent.
Embodiment 12
Composite bacteria agent is to DEHP contaminated soil remediation applications:
(1) for trying soil：Farmland rice soil, pH 5.62, organic matter 17.40g/kg, total nitrogen 0.79g/kg, total phosphorus
0.66g/kg crosses 60 mesh after air-drying.
(2) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated with respectively
In LB liquid medium, 30 DEG C of activation 18h；
(3) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, are cultivated respectively to bacterial population and are
1010cfu/mL；
(4) by 2 kinds of single bacteriums according to 2：1 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
(5) composite bacteria agent repairs DEHP contaminated soils
Setting DEHP pollution concentrations are 20mg/kg.600g soil is added in the beaker of 500mL, DEHP is added, stirring is equal
It is even, it is used in combination masking foil shading treatment, after aging for a week, adds bacterial strain YLYP1, XB, composite bacteria agent 60mL respectively into soil, stir
It mixes uniformly.Soil moisture content is adjusted to the 30% of field capacity, 30 DEG C of constant temperature incubation 15d, not add any microbial inoculum
Soil is blank control, 3 repetitions of above-mentioned each processing setting, the residual quantity of DEHP in measurement.The results show that in blank control
Degradation of DEHP in soil rate 27.1% is inoculated with single bacterium Bacillus megaterium YLYP1 degradation of DEHP in soil rates
36.5%, inoculation single bacterium Rhodococcus pyridinivorans XB degradation of DEHP in soil rate 65.3% adds compound bacteria
The degradation rate of DEHP is respectively 93.7% in the soil of agent.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, it is other it is any without departing from the spirit and principles of the present invention made by changes, modifications, substitutions, combinations, simplifications,
Equivalent substitute mode is should be, is included within the scope of the present invention.
Sequence table
<110>Ji'nan University
<120>One plant of phosphorus-solubilizing bacteria and its application with DEHP degradation bacterias composite bacteria agent and in soil improvement
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1450
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<220>
<223>The 16S rDNA sequences of Bacillus megaterium YLYP1
<400> 1
agcctggggc tctataatgc agtcgagcga ctgattagaa gcttgcttct atgacgttag 60
cggcggacgg gtgagtaaca cgtgggcaac ctgcctgtaa gactgggata acttcgggaa 120
accgaagcta ataccggata ggatcttctc cttcatggga gatgattgaa agatggtttc 180
ggctatcact tacagatggg cccgcggtgc attagctagt tggtgaggta acggctcacc 240
aaggcaacga tgcatagccg acctgagagg gtgatcggcc acactgggac tgagacacgg 300
cccagactcc tacgggaggc agcagtaggg aatcttccgc aatggacgaa agtctgacgg 360
agcaacgccg cgtgagtgat gaaggctttc gggtcgtaaa actctgttgt tagggaagaa 420
caagtacgag agtaactgct cgtaccttga cggtacctaa ccagaaagcc acggctaact 480
acgtgccagc agccgcggta atacgtaggt ggcaagcgtt atccggaatt attgggcgta 540
aagcgcgcgc aggcggtttc ttaagtctga tgtgaaagcc cacggctcaa ccgtggaggg 600
tcattggaaa ctggggaact tgagtgcaga agagaaaagc ggaattccac gtgtagcggt 660
gaaatgcgta gagatgtgga ggaacaccag tggcgaaggc ggctttttgg tctgtaactg 720
acgctgaggc gcgaaagcgt ggggagcaaa caggattaga taccctggta gtccacgccg 780
taaacgatga gtgctaagtg ttagagggtt tccgcccttt agtgctgcag ctaacgcatt 840
aagcactccg cctggggagt acggtcgcaa gactgaaact caaaggaatt gacgggggcc 900
cgcacaagcg gtggagcatg tggtttaatt cgaagcaacg cgaagaacct taccaggtct 960
tgacatcctc tgacaactct agagatagag cgttcccctt cgggggacag agtgacaggt 1020
ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc 1080
aacccttgat cttagttgcc agcatttagt tgggcactct aaggtgactg ccggtgacaa 1140
accggaggaa ggtggggatg acgtcaaatc atcatgcccc ttatgacctg ggctacacac 1200
gtgctacaat ggatggtaca aagggctgca agaccgcgag gtcaagccaa tcccataaaa 1260
ccattctcag ttcggattgt aggctgcaac tcgcctacat gaagctggaa tcgctagtaa 1320
tcgcggatca gcatgccgcg gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca 1380
ccacgagagt ttgtaacacc cgaagtcggt ggagtaaccg taaggagcta gccgcgtaag 1440
gtgaacaaat 1450
Claims (10)
1. one plant of phosphorus-solubilizing bacteria, it is characterised in that：Entitled Bacillus megaterium YLYP1, on 06 21st, 2017
The Guangdong Province microorganism fungus kind for being preserved in 5 building, the building of compound the 59th of Xianlie Middle Road, Guangzhou City 100 Guangdong Microbes Inst protects
Tibetan center, deposit number：GDMCC NO：60204.
2. application of the phosphorus-solubilizing bacteria described in claim 1 in dissolving difficultly-soluble phosphates.
3. according to the application described in claim 2, it is characterised in that：
The difficultly-soluble phosphates are calcium phosphate, aluminum phosphate or ferric phosphate.
4. a kind of composite bacteria agent, it is characterised in that：Including Bacillus megaterium YLYP1 described in claim 1 and
Rhodococcus pyridinivorans XB；
The Rhodococcus pyridinivorans XB were preserved in Xianlie Middle Road, Guangzhou City on 09 28th, 2016
Guangdong Province's Culture Collection of No. 100 5 building, the buildings of compound the 59th Guangdong Microbes Inst, deposit number：GDMCC
NO：60054.
5. composite bacteria agent according to claim 4, it is characterised in that：
The Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are made a concentration of
108~1010The bacteria suspension of cfu/mL.
6. composite bacteria agent according to claim 4 or 5, it is characterised in that：
Bacillus megaterium YLYP1 and Rhodococcus the pyridinivorans XB are according to volume ratio 2
~5：1~3 is mixed.
7. the preparation method of claim 4~6 any one of them composite bacteria agent, it is characterised in that include the following steps：
(1) Bacillus megaterium YLYP1 and Rhodococcus pyridinivorans XB are inoculated in LB respectively
In fluid nutrient medium, 28~30 DEG C of 12~18h of activation；
(2) the activated 2 kinds of single bacteriums of picking are inoculated in LB liquid medium respectively, and it is 10 to be cultivated respectively to bacterial population8~
1010cfu/mL；
(3) by 2 kinds of single bacteriums according to 2~5：1~3 volume ratio uniformly mixes to get multi-functional composite bacteria agent.
8. application of claim 4~6 any one of them composite bacteria agent in Soluble phosphorus and/or degradation DEHP.
9. application according to claim 8, it is characterised in that：
The composite bacteria agent is in NBRIP culture mediums, and titanium pigment content is up to 482.5~570.3mg/L；
The composite bacteria agent is 90.5%~100% to DEHP degradation rates in the MSM culture mediums containing DEHP.
10. application of claim 4~6 any one of them composite bacteria agent in improveing soil quality.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810316362.XA CN108587947B (en) | 2018-04-10 | 2018-04-10 | Phosphate solubilizing bacteria, composite microbial inoculum of phosphate solubilizing bacteria and DEHP degrading bacteria and application of phosphate solubilizing bacteria and DEHP degrading bacteria in soil improvement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810316362.XA CN108587947B (en) | 2018-04-10 | 2018-04-10 | Phosphate solubilizing bacteria, composite microbial inoculum of phosphate solubilizing bacteria and DEHP degrading bacteria and application of phosphate solubilizing bacteria and DEHP degrading bacteria in soil improvement |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108587947A true CN108587947A (en) | 2018-09-28 |
| CN108587947B CN108587947B (en) | 2020-05-08 |
Family
ID=63621618
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810316362.XA Active CN108587947B (en) | 2018-04-10 | 2018-04-10 | Phosphate solubilizing bacteria, composite microbial inoculum of phosphate solubilizing bacteria and DEHP degrading bacteria and application of phosphate solubilizing bacteria and DEHP degrading bacteria in soil improvement |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108587947B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109517756A (en) * | 2018-11-27 | 2019-03-26 | 中国科学院南海海洋研究所 | One plant of ocean phosphorus decomposing bacillus megaterium and its application |
| CN110669704A (en) * | 2019-11-19 | 2020-01-10 | 河南工业大学 | Efficient composite phosphorus-dissolving microbial inoculum and preparation method and application thereof |
| CN111484953A (en) * | 2019-01-28 | 2020-08-04 | 福建省农业科学院农业生物资源研究所 | Bacillus capable of promoting growth and dissolving phosphorus and application thereof |
| CN111575197A (en) * | 2020-04-08 | 2020-08-25 | 暨南大学 | PAEs degrading microbial inoculum, method for preventing and treating vegetable PAEs pollution and application |
| CN115386520A (en) * | 2022-08-26 | 2022-11-25 | 广东海洋大学 | Rhodococcus pyridinivorans RL-GZ01 strain and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673701A (en) * | 2014-11-17 | 2015-06-03 | 山东农业大学 | Preparation and application of enterobacter radicincitans and inoculant of enterobacter radicincitans |
| CN107686820A (en) * | 2017-09-11 | 2018-02-13 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of aerobic denitrifying bacteria and its application in water body denitrification |
-
2018
- 2018-04-10 CN CN201810316362.XA patent/CN108587947B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104673701A (en) * | 2014-11-17 | 2015-06-03 | 山东农业大学 | Preparation and application of enterobacter radicincitans and inoculant of enterobacter radicincitans |
| CN107686820A (en) * | 2017-09-11 | 2018-02-13 | 广东省微生物研究所（广东省微生物分析检测中心） | A kind of aerobic denitrifying bacteria and its application in water body denitrification |
Non-Patent Citations (1)
| Title |
|---|
| 陆瑞霞等: "溶磷菌的解磷促生效应及其在未来农业中的应用前景", 《现代园艺》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109517756A (en) * | 2018-11-27 | 2019-03-26 | 中国科学院南海海洋研究所 | One plant of ocean phosphorus decomposing bacillus megaterium and its application |
| CN111484953A (en) * | 2019-01-28 | 2020-08-04 | 福建省农业科学院农业生物资源研究所 | Bacillus capable of promoting growth and dissolving phosphorus and application thereof |
| CN110669704A (en) * | 2019-11-19 | 2020-01-10 | 河南工业大学 | Efficient composite phosphorus-dissolving microbial inoculum and preparation method and application thereof |
| CN110669704B (en) * | 2019-11-19 | 2023-06-27 | 河南工业大学 | Efficient composite phosphorus-dissolving microbial inoculum and preparation method and application thereof |
| CN111575197A (en) * | 2020-04-08 | 2020-08-25 | 暨南大学 | PAEs degrading microbial inoculum, method for preventing and treating vegetable PAEs pollution and application |
| CN115386520A (en) * | 2022-08-26 | 2022-11-25 | 广东海洋大学 | Rhodococcus pyridinivorans RL-GZ01 strain and application thereof |
| CN115386520B (en) * | 2022-08-26 | 2023-11-14 | 广东海洋大学 | Rhodococcus pyridine-philic RL-GZ01 strain and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108587947B (en) | 2020-05-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108587947A (en) | One plant of phosphorus-solubilizing bacteria and its application with DEHP degradation bacterias composite bacteria agent and in soil improvement | |
| CN108546658A (en) | One plant of phosphorus-solubilizing bacteria and its compound microbial inoculum and application with DEHP degradation bacterias | |
| CN111117910B (en) | Enterobacter ludwigii PN6 and application thereof | |
| de Oliveira Mendes et al. | Fermentation liquid containing microbially solubilized P significantly improved plant growth and P uptake in both soil and soilless experiments | |
| CN104263684B (en) | A kind of product siderophore series bacillus and application thereof | |
| CN105964680B (en) | A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof | |
| CN108531424A (en) | One plant of efficient phosphorus-dissolution bacterium and its application for soil improvement | |
| CN107629978A (en) | A kind of Pseudomonas nitroreducens and its application in the colony induction signaling molecule DSF that degrades | |
| CN108893421B (en) | Bacillus fusiformis and application thereof in reclamation ecological reconstruction of mining area | |
| CN102652957A (en) | Remediation method of soil polycyclic aromatic hydrocarbon by combining surfactant producing bacteria and mycorrhiza | |
| CN108441450B (en) | Complex microbial inoculant with phosphorus dissolving and DEHP degrading functions and application thereof | |
| CN104673715A (en) | Enteric bacilli with fixing effect on cadmium capable of promoting plant growth and application of enteric bacilli | |
| CN106434472A (en) | Stenotrophomonas sp and application thereof | |
| CN114480183A (en) | Bacillus aryabhattai HZ18-3 and application thereof | |
| CN104630087A (en) | Maize growth-promoting rhizobacteria YM4 and application thereof | |
| CN104152385A (en) | Bacillus mojavensis QLY002 strain, microbial inoculum, preparation method of microbial inoculum and use of Bacillus mojavensis QLY002 strain and microbial inoculum | |
| CN109576181A (en) | A kind of modified illite powder microbial bacterial agent and the preparation method and application thereof | |
| CN110257293A (en) | P. amylolyticus KY15, microbial inoculum, application and the product using it | |
| CN106348887A (en) | Method for preparing amino acid liquid water-soluble fertilizer through compound stepped fermentation of seaweed | |
| CN107267414B (en) | One plant of plant azotobacteria DJG211 and its application in improving soil and plant vigor | |
| CN109576177A (en) | A kind of China microbot bacterial strain SM8 and its application in salt tolerant growth-promoting | |
| CN108672490A (en) | A kind of microorganism passivating method for remediating heavy metal lead-contaminated soil | |
| CN105907399B (en) | A kind of cadmium pollution renovation agent and its with the application in plant combined reparation agricultural land soil | |
| CN108239610A (en) | One plant of low temperature resistant phosphate solubilizing bacteria and its method for carrying out farmland phosphorus decomposing | |
| CN105132332B (en) | One strain of gluconacetobacter and its application as plant growth-promoting bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |