CN108941192A - A kind of arsenic in soil method for governing pollution and application - Google Patents
A kind of arsenic in soil method for governing pollution and application Download PDFInfo
- Publication number
- CN108941192A CN108941192A CN201810721107.3A CN201810721107A CN108941192A CN 108941192 A CN108941192 A CN 108941192A CN 201810721107 A CN201810721107 A CN 201810721107A CN 108941192 A CN108941192 A CN 108941192A
- Authority
- CN
- China
- Prior art keywords
- arsenic
- soil
- grams
- volume
- pollution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
Abstract
A kind of arsenic in soil method for governing pollution and application, the technology belong to ecological environmental protection field.The technology is realized by following steps: starch being added in the bioreactor, soya-bean cake, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium carbonate, ferrous sulfate etc., add enough water, stirring, it mixes, heat sterilization, it is cooling, it is inoculated with the bacterial strains such as Flavobacterium, fermentation, it is cooling, mixture, it is canned, in being manured into soil, naturally culture 8-20 months, it can continue to convert escaping gas for the arsenic in soil, it is evaporate into air from soil, to remove the arsenic in soil, eliminate soil and groundwater arsenic pollution, soil protection environment, soil protection production and ecological functions, protect groundwater environment, it ensures food safety.
Description
Technical field
The invention belongs to ecological environmental protection field, in particular to a kind of arsenic in soil method for governing pollution and application.
Background technique
Arsenic is a kind of environmental contaminants that toxicity is very strong.Arsenic in natural soils is mainly derived from soil parent material.By
During rock weathering forms soil, the content (relative to soil parent material) of arsenic has apparent enrichment.Mankind's activity such as mining,
It smelts, dirty filling, apply fertilizer, the application of insecticide and timber preservative all may cause soil arsenic pollution.Excessive Arsenic is accumulated in soil,
It will cause crop failure and quality decline, while may cause water source and atmosphere pollution, and the way such as food chain and water body can be passed through
Diameter enters human body.In recent years studies have shown that the arsenic of low amounts can also seriously endanger human health.
2016, it was one that " Soil Pollution Control action plan ", " Soil Pollution Control action plan ", which is formally issued and implemented,
The item huge soil pollution recovery project of scale of investment, also will be a long-term engineering.Due to soil remediation Its Relevant Technology Standards
The many difficulties implemented with soil remediations such as the shortages of basic data, interior in recent years, China's Soil Pollution Control also needs first to lead to
It crosses pilot demonstration and part is carried out, then realize the process of scale development.Basic data and the accumulation and reparation for repairing experience
The deposit of technology by be China's current stage soil remediation vital task.
China is arsenic minerals big country, arsenic minerals be widely distributed in the Central-South and southwestern Hunan in China, Yunnan, Guangxi, Guangdong etc. save,
Area.Arsenic minerals be easy to cause the pollution of neighbouring soil and water body in exploitation or smelting process.According to Wang Zhengang etc. to Shimen Realgar Mine
3 neighbouring villages investigate discovery, and soil arsenic content is 84~296mg/kg, this shows soil by different degrees of pollution.Arsenic
Water body migration stain large area agricultural land soil can also be passed through.Because paddy field mostly uses irrigation canals and ditches to irrigate, near mining and smeltery
Water paddy soil contamination phenomenon is on the rise, if there is large area in Hunan Changning county by arsenic polluted water rice field, arsenic content is 92~
840mg/kg；There are more than 8000 hectares of paddy fields by arsenic pollution in Hubei.Cause in Bangladesh since underground water is excessively high containing arsenic
70000000 people's drinking water arsenic contents are exceeded；With this irrigation water of well water containing arsenic rice field, allow for 0 in the time less than 10 years~
15cm soil horizon arsenic content is increased to 83mg/kg, and local arsenic in soil background value is only 4~8mg/kg.
The biological effect and its toxicity of arsenic, mainly due to sulfydryl (- SH), cystine and the half Guang ammonia in arsenic and zymoprotein
The amino (- NH) of sour sulfur-bearing has very strong affinity, so that histocyte breathing is obstructed in conjunction with after, mitochondrial swelling, to make generation
Thank stopping, cell death.For one kind, " the known mankind are carcinogenic by the World Health Organization (WHO) and U.S.EPA (EPA) deciding grade and level for arsenic
Substance ", human body exposes arsenic for a long time can lead to the canceration of the internal organs such as cutaneum carcinoma and kidney, liver, bladder.The approach of arsenic poisoning is main
Enter digestive system by drinking-water and food, the exhaust gas containing arsenic of industrial discharge enters the respiratory system.As3+It can be with the mercapto of human body enzyme
Base junction is closed, inhibitory enzyme activity, leads to carbohydrate metabolism disturbance, the content of acetone in blood and tissue increases and nerve endings function
Disorder.As5+Toxic effect is slower, it can destroy mitochondria oxidative phosphorylation, phosphate can be replaced to generate unstable
Arsenate, it may appear that the sequelae such as polyneuritis, myelitis, hypoplastic anemia.The toxic dose of adult arsenious acid
For 5~50mg/kg, lethal dose is 100~300mg/kg；The half lethal concentration (LD50) of arsenious acid is 10mg/kg, and arsenic acid
LD50 be 138mg/kg.
With the increase of arsenic amount, soil bacteria and actinomyces sum are substantially reduced, and arsenic is to Soil hydrolase system (urea
Enzyme, phosphatase and protease) and oxidoreducing enzyme (dehydrogenase, polyphenol oxidase and catalase) have different degrees of suppression
System.Arsenic inhibits CO in Soil Microorganism activity2Generation, significantly reduce the activity of denitrifying bacteria.Arsenic is to soil fixed nitrogen
Bacterium, phosphate-solubilizing bacteria and cellulose-decomposing bacteria have inhibiting effect, and wherein trichoderma and Bacterium anthracoides are most sensitive to arsenic, and Soybean Root
Tumor bacterium and the rigid spirillum patience highest containing rouge.
Arsenic pollution and arsenic species are a global problems.Japanese government is by arsenic poisoning and lead poisoning, minamata disease, bone
Seriously pollution disease is mutually arranged side by side for pain disease etc., is declared as fourth environmental disease.The toxicity of general arsenic is successively are as follows: and arsenic hydride > trivalent inorganic arsenic >
Pentavalent inorganic arsenic > organo-arsenic > element arsenic.Arsenic is plant non-essential element, in general plant As content mobility scale be 0.01~
5mg/kg.Plant can absorb arsenic from external environment in growth and development process, and the arsenic of low amounts can stimulate the growth of plant.But
Higher arsenic content can generate toxic effect to plant in soil, endanger its growth and development, make crop yield and quality decline.
The soil that property is different, purposes is different, pollution level is different, the technology and methods repaired are not also identical.It is common
Arsenic in soil pollution control recovery technique has following several: immobilization, stabilization technology, passivating technique, Soil leaching technology, original position
Electric repairing technique, gram local method, bioremediation technology etc..Fixation, Dunhua, stabilization technology are at low cost, but materials curing agent
It is a large amount of using the structure that can destroy soil, therefore, which is not suitable for the soil remediation of large area.Soil leaching technology is used
Leacheate elution, the method easily cause the leaching loss and precipitating of certain nutrients, and therefore, this method is suitable for the small weight of area
The soil remediation of metallic pollution.Ma et al. has found a kind of plant-Pteris vittata L in the middle part of Fla., can super enrichment
Arsenic is planted Pteris vittata L in laboratory, is cultivated 6 weeks, the content of arsenic has reached 22630mg/kg in accessory pinna.Chen Tongbin etc., Wei Chao
Sun etc. have also discovered the super enriching plant-Pteris vittata L and cretan brake of arsenic in Hunan China.Recently it was also discovered that many plants
Object can be good at being enriched with arsenic, such as: Creeping bentgrass, Meng Tana chrysanthemum, knotweed vehicle, Bermuda grass etc..But these plants are mostly
Shade plant among long term growth virgin soil out of office open country, is cultivated for large area agricultural land soil arsenic, is exposed to the sun intolerant to sunburst and pH
The great change of value, often undergrowth, repairing effect is bad, simultaneously because super build-up properties are substantially after continuous multi-generation vegetative propagation
It degenerates, loses repair function.
This technology uses multiple-microorganism mix bacterium agent rehabilitating soil arsenic, in conjunction with soil moisture and oxidation-reduction potential tune
The compound of arsenic in soil and arsenic is restored and/or is oxidized to methyl arsenic, dimethyl arsenic, arsenic trimethide and Shenization Qing Den gas by control,
It is evaporated from soil, so that soil arsenic pollution, soil protection and groundwater environment are removed, the compound height produced using this law
It is high to imitate microbial inoculum treatment effeciency, at low cost, environment friendly is high, and it is easy to operate, without secondary pollution, large-scale industry easy to accomplish
Change application.
Summary of the invention
The technical issues of solution: the present invention is directed to soil arsenic pollution, especially agricultural land soil and underground water, Metallurgical Factory, gold
Belong to stope and chemical plant contaminated sites, invents a kind of high efficiency composition microbial inoculum, it is within a short period of time that soil arsenic pollution is most of
The gaseous volatilizations such as methyl arsenic are aoxidized and/or are reduced into, to remove harm of the soil As to environment and the mankind.
Technical solution: a kind of arsenic in soil method for governing pollution and application, step be a kind of arsenic in soil method for governing pollution and
Using, it is characterised in that step are as follows: a. is added 100-800 grams of starch in 10 liters of bioreactors, 60-250 grams of beancake powder,
40-150 grams of dipotassium hydrogen phosphate, 30-150 grams of potassium dihydrogen phosphate, 10-100 grams of calcium carbonate powder, 5-60 grams of ferrous sulfate, 10-50
Gram magnesium sulfate, 7000 milliliters of water start blender stirring, start heating steam pipe, heat mixed solution, and lower 126 DEG C of 0.1Mpa
It is kept for 40 minutes, sterilizing terminates, and is cooled to the Flavobacterium of 30 DEG C of inoculations 0.5-8% (mass/volume), keeps stirring, blender
50-120 revs/min of revolving speed, ventilatory capacity 0.5-1 goes up to the air gas/liter liquid (volume/volume/minute), and constant temperature is mixed 24-48
Hour, fermentation ends put tank.Similarly, it is inoculated with 0.5-8%'s (mass/volume) respectively in above-mentioned same liquid fermentation medium
Purple sulfur bacteria, Aeromonas media, start blender, 50-120 revs/min of agitator speed, ventilatory capacity 0.5-1 go up to the air gas/
It rises liquid (volume/volume/minute), constant temperature is mixed 24-48 hours, and fermentation ends put tank.B. by above-mentioned a, treated 3
The different fermentation liquid concentration of kind, filtering retain filtrate, viable bacteria and spore content >=200,000,000/milliliter in filtrate are kept, by 3 kinds of bacterium solutions
It is mixed into composite bacteria liquid preparation in equal volume.C. by above-mentioned b, treated that composite bacteria agent sprays agricultural land soil surface, per acre uniformly spray
50-200 kilograms of composite bacteria agent is applied, is poured water immediately, so that soil surface has ponding 10cm or more, and keeps soil surface humidity shape
State 20 days or more, spray 50-200 kilograms of composite bacteria agent for the second time, and pour water immediately so that agricultural land soil surface layer ponding 10cm with
On, it maintains field to have ponding 20 days or more, according to arsenic in soil pollution level, repeat spraying microbial inoculum number 4-10 times, sprays every time
It will pour water immediately after microbial inoculum, and keep soil surface ponding 10cm or more, and keep 20 days moist or more, in favor of in microbial inoculum
Microorganism is proliferated in the soil, biological oxidation/reduction arsenic becomes the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride.
A kind of composite bacteria agent of any rehabilitating soil arsenic pollution and its farmland, refuse landfill, municipal sludge plus
Petrol station, paper mill, stope, electroplate factory, nearby soil pollution arsenic contaminates for electrolysis plant, metallurgical works, contaminated sites and farm
Application in reparation.3 kinds of microorganisms can be happy from Chinese Academy of Sciences bacterium guarantor center, the Chinese Academy of Agricultural Sciences bacterium guarantor center, Nanjing used in this law
Ecological environment Science and Technology Ltd. can be helped to buy.
The utility model has the advantages that the present invention can eliminate arsenic pollution object in soil and groundwater to greatest extent, eliminate to greatest extent
Soil arsenic pollution and its destruction to environment and the harm to the mankind, are conducive to Ecological Sustainable Development.
The present invention is compared with prior art:
1) the soil arsenic pollution composite bacteria agent researched and developed in the present invention, production method simplicity, technically simple easy, operability
By force, low in cost, practical；
2) present invention removes soil arsenic pollution using microbiological oxidation reduction technique, can be really soil and/or underground
Arsenic is converted into gaseous volatilization and goes out in water, reduces the arsenic polluted in soil to the maximum extent, fundamentally eliminates in soil
Arsenic is compared with currently used single solidification hair/deactivation method, is real removal soil arsenic pollution, residue and secondary dirt
It is seldom to contaminate object, selected 3 kinds of compound bacterias be all it is naturally occurring in soil natural circle, do not pollute with other harm, be a kind of
Eco-friendly arsenic pollution administering method is the current best approach.
Detailed description of the invention
Fig. 1 is using this technology processing arsenic in soil pollution course comparison.
Specific embodiment
Below in conjunction with example, the invention will be further described:
Embodiment 1:
1. 60 grams of starch, 70 grams of beancake powders, 10 grams of calcium carbonate powders, 35 grams of phosphoric acid are added in 10 liters of bioreactors
Hydrogen dipotassium, 35 grams of potassium dihydrogen phosphates, 15 grams of ferrous sulfate, 15 grams of magnesium sulfate, 7000 milliliters of water, stirring are opened after all mixing
Dynamic heating steam pipe heats mixed solution, and lower 126 DEG C of 0.1Mpa are kept for 40 minutes, and sterilizing terminates, and is cooled to 30 DEG C of inoculations
The Flavobacterium of 0.5% (mass/volume), starts blender, and 50 revs/min of agitator speed, ventilatory capacity 0.5 goes up to the air gas/liter liquid
(volume/volume/minute), constant temperature are mixed 48 hours, and fermentation ends put tank.Similarly, in same liquid fermentation medium point
Not Jie Zhong the purple sulfur bacteria of 0.5% (W/V), Aeromonas media, start blender, 50 revs/min of agitator speed, ventilation
0.5 lift-off gas/liter liquid (volume/volume/minute) of amount, constant temperature are mixed 48 hours, and fermentation ends put tank.
2. by above-mentioned 1, treated that 3 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, keeps viable bacteria and bud in filtrate
3 kinds of bacterium solutions are mixed into composite bacteria liquid preparation by spore content >=200,000,000/milliliter in equal volume.
3. by above-mentioned 2, treated that composite bacteria agent sprays agricultural land soil surface, uniformly sprays composite bacteria agent 200,000 per acre
Gram, it pours water immediately, so that soil surface has ponding 10cm or more, and is kept for soil surface dampness 20 days or more, second
200 kilograms of composite bacteria agents are sprayed, and are poured water immediately, so that agricultural land soil surface layer ponding 10cm or more, maintains field to have ponding 20
More than it, whether seriously polluted according to arsenic in soil, repeat spraying microbial inoculum number 8 times will pour water immediately after spraying microbial inoculum every time,
And keep soil surface ponding 10cm or more, and keep moist 20 days upwards, in favor of in microbial inoculum microorganism be proliferated in the soil,
Biological oxidation/reduction arsenic becomes the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride.Major part in soil is solvable
The ionic condition of property, indissoluble state, ADSORPTION STATE arsenic are converted into volatile monomethyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride,
It escapes from soil or underground water, gets into the air, significantly reduce the content of arsenic.
Embodiment 2:
1. 250 grams of starch, 170 grams of beancake powders, 25 grams of calcium carbonate powders, 60 grams of phosphorus are added in 10 liters of bioreactors
Sour hydrogen dipotassium, 50 grams of potassium dihydrogen phosphates, 30 grams of ferrous sulfate, 30 grams of magnesium sulfate, 7000 milliliters of water, stirring, after all mixing
Heating steam pipe is started, mixed solution is heated, lower 126 DEG C of 0.1Mpa are kept for 40 minutes, and sterilizing terminates, and is cooled to 30 DEG C of inoculations
The Flavobacterium of 4% (mass/volume), starts blender, and 95 revs/min of agitator speed, ventilatory capacity 0.8 goes up to the air gas/liter liquid (body
Product/volume), constant temperature is mixed 38 hours, and fermentation ends put tank.Similarly, it is inoculated with respectively in same liquid fermentation medium
The purple sulfur bacteria of 4% (mass/volume), Aeromonas media, start blender, and 95 revs/min of agitator speed, ventilatory capacity
0.8 lift-off gas/liter liquid (volume/volume/minute), constant temperature are mixed 36 hours, and fermentation ends put tank.
2. by above-mentioned 1, treated that 3 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, keeps viable bacteria and bud in filtrate
Three kinds of bacterium solutions are mixed into composite bacteria liquid preparation by spore content >=200,000,000/milliliter in equal volume.
3. by above-mentioned 2, treated that composite bacteria agent sprays agricultural land soil surface, uniformly sprays composite bacteria agent 100,000 per acre
Gram, it pours water immediately, so that soil surface has ponding 10cm or more, and is kept for soil surface dampness 20 days or more, second
Double centner composite bacteria agent is sprayed, and is poured water immediately, so that agricultural land soil surface layer ponding 10cm or more, maintains field to have ponding 20
More than it, according to arsenic in soil pollution level, repeat spraying microbial inoculum number 56 times will pour water immediately after spraying microbial inoculum every time, and
Soil surface ponding 10cm or more is kept, and is kept for moist 20 days upwards, so that microorganism be proliferated in the soil, gives birth in microbial inoculum
Object oxidation/reduction arsenic becomes the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride.It is most of soluble in soil
Ionic condition, indissoluble state, ADSORPTION STATE arsenic be converted into volatile monomethyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride, from
Soil or underground water escape, get into the air, significantly reduce the content of arsenic
Embodiment 3
1. 600 grams of starch is added in 10 liters of bioreactors, 240 grams of beancake powders, 60 grams of calcium carbonate powders, 110 grams
Dipotassium hydrogen phosphate, 110 grams of potassium dihydrogen phosphates, 40 grams of ferrous sulfate, 60 grams of magnesium sulfate, 7000 milliliters of water, stirring, to whole mixings
After start heating steam pipe, heat mixed solution, lower 126 DEG C of 0.1Mpa are kept for 40 minutes, and sterilizing terminates, and is cooled to 30 DEG C of inoculations
The Pseudomonas putidas of 8% (mass/volume), starts blender, 120 revs/min of agitator speed, ventilatory capacity 1 go up to the air gas/liter
Liquid (volume/volume/minute), constant temperature are mixed 24 hours, and fermentation ends put tank.Similarly, in same liquid fermentation and culture
Base is inoculated with Bacillus foecalis alkaligenes, the Flavobacterium of 8% (mass/volume) respectively, starts blender, 120 revs/min of agitator speed, leads to
Tolerance 1 is gone up to the air gas/liter liquid (volume/volume/minute), and constant temperature is mixed 24 hours, and fermentation ends put tank.
2. by above-mentioned 1, treated that 3 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, keeps viable bacteria and bud in filtrate
3 kinds of bacterium solutions are mixed into composite bacteria liquid preparation by spore content >=200,000,000/milliliter in equal volume.
3. by above-mentioned 2, treated that composite bacteria agent sprays agricultural land soil surface, uniformly sprays 50 kilograms of composite bacteria agent per acre,
It pours water, so that soil surface has ponding 10cm or more, and is kept for soil surface dampness 20 days or more, sprays for the second time immediately
50 kilograms of composite bacteria agents, and pouring water immediately so that agricultural land soil surface layer ponding 10cm or more, maintain field to have ponding 20 days with
On, whether seriously polluted according to arsenic in soil, repeat spraying microbial inoculum number 4 times will pour water immediately after spraying microbial inoculum every time, and protect
Hold soil surface ponding 10cm or more, and keep moist 20 days upwards, in favor of in microbial inoculum microorganism be proliferated in the soil, it is biological
Redox arsenic becomes the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride.In soil it is most of soluble from
Sub- state, indissoluble state, ADSORPTION STATE arsenic are converted into volatile monomethyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride, from soil
Or underground water escape, it gets into the air, significantly reduces the content of arsenic
Application example of the invention:
By the technology of the present invention be used to pollute various heavy soil remediation using.
1, it referring to embodiment 1, is handled using this technology and contains As contaminated soil, handled 12 months by continuous 10 times
Afterwards, As, which is remained, with the control soil of this technology not being used to compare, in soil reduces by 46% (Fig. 1)；
2, it referring to embodiment 2, is handled using this technology and contains As contaminated soil, handled 12 months by continuous 10 times
Afterwards, As, which is remained, with the control soil of this technology not being used to compare, in soil reduces 71%；
3, it referring to embodiment 3, is handled using this technology and contains As contaminated soil, handled 12 months by continuous 10 times
Afterwards, As, which is remained, with the control soil of this technology not being used to compare, in soil reduces 76%.
Claims (5)
1. a kind of arsenic in soil method for governing pollution and application, it is characterised in that step are as follows:
A. 100-800 grams of starch, 60-250 grams of beancake powder, 40-150 grams of phosphoric acid hydrogen two are added in 10 liters of bioreactors
Potassium, 30-150 grams of potassium dihydrogen phosphate, 10-100 grams of calcium carbonate powder, 5-60 grams of ferrous sulfate, 10-50 grams of magnesium sulfate, 7000 millis
Water is risen, blender stirring is started, starts heating steam pipe, heats mixed solution, lower 126 DEG C of 0.1Mpa are kept for 40 minutes, sterilizing
Terminate, is cooled to the Flavobacterium of 30 DEG C of inoculations 0.5-8% (mass/volume), keeps stirring, 50-120 revs/min of agitator speed
Clock, ventilatory capacity 0.5-1 go up to the air gas/liter liquid (volume/volume/minute), and constant temperature is mixed 24-48 hours, and fermentation ends are put
Tank.Similarly, purple sulfur bacteria, the centre gas of 0.5-8% (mass/volume) are inoculated with respectively in above-mentioned same liquid fermentation medium
Monad, starts blender, 50-120 revs/min of agitator speed, ventilatory capacity 0.5-1 go up to the air gas/liter liquid (volume/volume/
Minute), constant temperature is mixed 24-48 hours, and fermentation ends put tank.
B. by above-mentioned a, treated that 3 kinds of different fermentation liquids are concentrated, and filtering retains filtrate, and viable bacteria and gemma in filtrate is kept to contain
>=2 hundred million/milliliters are measured, 3 kinds of bacterium solutions are mixed into composite bacteria liquid preparation in equal volume.
C. by above-mentioned b, treated that composite bacteria agent sprays agricultural land soil surface, uniformly sprays 50-200 kilograms of composite bacteria agent per acre,
It pours water, so that soil surface has ponding 10cm or more, and is kept for soil surface dampness 20 days or more, sprays for the second time immediately
50-200 kilograms of composite bacteria agent, and pour water immediately, so that agricultural land soil surface layer ponding 10cm or more, maintains field dampness 20
More than it, according to arsenic in soil pollution level, repeat spraying microbial inoculum number 4-10 times, it will pour water immediately after spraying microbial inoculum every time,
And soil surface ponding 10cm or more is kept, and keep 20 days moist or more, in favor of microbial nutrition body and gemma in microbial inoculum
Proliferation, biological oxidation and/or reduction arsenic become the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride in the soil.
2. a kind of arsenic in soil method for governing pollution and application according to claim 1, it is characterised in that the method is 10
The starch of 100-800 grams of addition in liter bioreactor, 60-250 grams of beancake powder, 40-150 grams of dipotassium hydrogen phosphate, 30-150 grams
Potassium dihydrogen phosphate, 10-100 grams of calcium carbonate powder, 5-60 grams of ferrous sulfate, 10-50 grams of magnesium sulfate, 7000 milliliters of water are started and are stirred
Device stirring is mixed, heating steam pipe is started, heats mixed solution, lower 126 DEG C of 0.1Mpa are kept for 40 minutes, and sterilizing terminates, and is cooled to
The Flavobacterium of 30 DEG C of inoculations 0.5-8% (mass/volume), keeps stirring, 50-120 revs/min of agitator speed, ventilatory capacity
0.5-1 goes up to the air gas/liter liquid (volume/volume/minute), and constant temperature is mixed 24-48 hours, and fermentation ends put tank.Similarly,
It is inoculated with the purple sulfur bacteria of 0.5-8% (mass/volume), Aeromonas media respectively in above-mentioned same liquid fermentation medium,
Blender is started, 50-120 revs/min of agitator speed, ventilatory capacity 0.5-1 goes up to the air gas/liter liquid (volume/volume/minute),
Constant temperature is mixed 24-48 hours, and fermentation ends put tank.
3. a kind of arsenic in soil method for governing pollution and application according to claim 1, it is characterised in that, will be upper in the method
A is stated treated 3 kinds of different fermentation liquids concentrations, filtering retains filtrate, keeps viable bacteria and spore content >=200,000,000/milli in filtrate
It rises, 3 kinds of bacterium solutions is mixed into composite bacteria liquid preparation in equal volume.
4. a kind of arsenic in soil method for governing pollution and application according to claim 1, it is characterised in that the method, it will be above-mentioned
Treated that composite bacteria agent sprays agricultural land soil surface by b, uniformly sprays 50-200 kilograms of composite bacteria agent per acre, pours water immediately, makes
Obtaining soil surface has ponding 10cm or more, and is kept for soil surface dampness 20 days or more, sprays 50-200 kilograms for the second time
Composite bacteria agent, and pour water immediately, so that agricultural land soil surface layer ponding 10cm or more, maintains field to have ponding 20 days or more, according to
Arsenic in soil seriously polluted degree repeat spraying microbial inoculum number 4-10 times, will pour water immediately after spraying microbial inoculum every time, and keep soil
Earth surface area water 10cm or more, and being kept for moist 20 days or more, in favor of in microbial inoculum microorganism be proliferated in the soil, biological oxidation
And/or reduction arsenic becomes the gases such as methyl arsenic, dimethyl arsenic, arsenic trimethide and arsenic hydride.
5. any a kind of improvement arsenic in soil method for governing pollution of Claims 1 to 4 and its in farmland As polluted soil, rubbish
Landfill yard, municipal sludge, gas station, stope, electroplate factory, electrolysis plant, metallurgical works, contaminated soil of site pollution arsenic dye are repaired
Application on multiple.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810721107.3A CN108941192A (en) | 2018-06-29 | 2018-06-29 | A kind of arsenic in soil method for governing pollution and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810721107.3A CN108941192A (en) | 2018-06-29 | 2018-06-29 | A kind of arsenic in soil method for governing pollution and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108941192A true CN108941192A (en) | 2018-12-07 |
Family
ID=64485488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810721107.3A Pending CN108941192A (en) | 2018-06-29 | 2018-06-29 | A kind of arsenic in soil method for governing pollution and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108941192A (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101259978A (en) * | 2007-12-28 | 2008-09-10 | 凌亮 | Method and device for synchronously treating steel pickling waste water and coking waste water |
| CN102757896A (en) * | 2012-07-10 | 2012-10-31 | 徐州奇缘生态农业发展有限公司 | Efficient microbial soil remediation agent |
| CN104119925A (en) * | 2013-04-25 | 2014-10-29 | 成都新朝阳作物科学有限公司 | Soil heavy metal repairing agent and application thereof to jointly contaminated soil |
| CN105316248A (en) * | 2014-07-21 | 2016-02-10 | 康源绿洲生物科技（北京）有限公司 | Microbial soil repairing agent and preparation method thereof |
| CN106495939A (en) * | 2016-10-14 | 2017-03-15 | 上海诺同农业科技有限公司 | Heavy metal-polluted soil renovation agent, preparation method and application |
| CN106754398A (en) * | 2016-11-18 | 2017-05-31 | 青岛蔚蓝生物集团有限公司 | It is a kind of to visit Lay mould and its application in agricultural production |
| CN108048102A (en) * | 2017-12-12 | 2018-05-18 | 中科绿洲（北京）生态工程技术有限公司 | A kind of salt-soda soil microorganism renovation agent and preparation method thereof and application method |
| CN108191480A (en) * | 2018-03-09 | 2018-06-22 | 易虹 | A kind of biological organic fertilizer and preparation method thereof |
| CN108192625A (en) * | 2017-12-25 | 2018-06-22 | 冷梦思 | A kind of As polluted soil in-situ immobilization agent and its preparation method and application |
-
2018
- 2018-06-29 CN CN201810721107.3A patent/CN108941192A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101259978A (en) * | 2007-12-28 | 2008-09-10 | 凌亮 | Method and device for synchronously treating steel pickling waste water and coking waste water |
| CN102757896A (en) * | 2012-07-10 | 2012-10-31 | 徐州奇缘生态农业发展有限公司 | Efficient microbial soil remediation agent |
| CN104119925A (en) * | 2013-04-25 | 2014-10-29 | 成都新朝阳作物科学有限公司 | Soil heavy metal repairing agent and application thereof to jointly contaminated soil |
| CN105316248A (en) * | 2014-07-21 | 2016-02-10 | 康源绿洲生物科技（北京）有限公司 | Microbial soil repairing agent and preparation method thereof |
| CN106495939A (en) * | 2016-10-14 | 2017-03-15 | 上海诺同农业科技有限公司 | Heavy metal-polluted soil renovation agent, preparation method and application |
| CN106754398A (en) * | 2016-11-18 | 2017-05-31 | 青岛蔚蓝生物集团有限公司 | It is a kind of to visit Lay mould and its application in agricultural production |
| CN108048102A (en) * | 2017-12-12 | 2018-05-18 | 中科绿洲（北京）生态工程技术有限公司 | A kind of salt-soda soil microorganism renovation agent and preparation method thereof and application method |
| CN108192625A (en) * | 2017-12-25 | 2018-06-22 | 冷梦思 | A kind of As polluted soil in-situ immobilization agent and its preparation method and application |
| CN108191480A (en) * | 2018-03-09 | 2018-06-22 | 易虹 | A kind of biological organic fertilizer and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107488620B (en) | Cadmium-polluted rice field soil microbial passivator | |
| CN110711769A (en) | Grading heavy metal soil in-situ remediation agent and remediation method thereof | |
| CN106734188B (en) | Micro-ecological restoration method and composition for heavy metal pollution of farmland | |
| CN107774704A (en) | A kind of method of heavy metal copper in plant animal microbial association rehabilitating soil | |
| CN108192631B (en) | A kind of charcoal base flora composite soil conditioner and its preparation and application | |
| CN104707864B (en) | Compound photosynthetic bacteria preparation for enhancing phytoremediation for heavy metal pollution of soil and preparation method thereof | |
| CN107457253A (en) | A kind of method of medicament and reparation As polluted soil for As polluted soil reparation | |
| CN108941193A (en) | A kind of arsenic in soil cadmium lead zinc combined pollution bioengineering restorative procedure and application | |
| Gong et al. | Study on the effect mechanism of Arbuscular Mycorrhiza on the absorption of heavy metal elements in soil by plants | |
| CN106701097A (en) | Repair agent for treating heavy metal contaminated soil and application thereof | |
| CN105255782A (en) | Cellulosimicrobium cellulans with reducing capacity on hexavalent chromium and application | |
| CN107597060A (en) | Adsorbent based on photosynthetic Bacillus processing heavy metal-polluted soil and preparation method thereof | |
| CN102652957A (en) | Remediation method of soil polycyclic aromatic hydrocarbon by combining surfactant producing bacteria and mycorrhiza | |
| CN109182178A (en) | One plant of bacterial strain and its application in reparation mild or moderate chromium-polluted soil in situ with chromium tolerance and Cr (VI) removal ability | |
| CN108409080A (en) | A kind of in-situ sediment remediation particle and preparation method thereof, application method, application | |
| CN104226679A (en) | Method for repairing contaminated soil in coal-to-gas plant by adopting microbe-plant combined way | |
| CN105013815A (en) | Biological remediation method for polycyclic aromatic hydrocarbon and heavy metal compound contaminated soil | |
| CN107236685A (en) | A kind of method that utilization sludge produces γ polyglutamic acid organic fertilizers | |
| CN104928213B (en) | A kind of atypical watt Salmonella for being used for repairing environment heavy metal pollution and its application | |
| Zhu et al. | Potential of arsenate-reducing bacterial inoculants to enhance field-scale remediation of arsenic contaminated soils by Pteris vittata L | |
| CN106244155A (en) | A kind of soil Cr pollution amelioration agent | |
| Levanon et al. | Chemical, physical and microbiological considerations in recycling spent mushroom substrate | |
| CN108838205A (en) | A kind of biological amassing capacity of rehabilitating soil arsenic pollution | |
| CN108114978B (en) | A kind of method of the efficient rehabilitating soil of chemistry-microorganism | |
| CN106244154A (en) | A kind of Lead Pollution in Soil renovation agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20181207 |
|
| WD01 | Invention patent application deemed withdrawn after publication |