CN103910578B - Rooting nutritional type microbial fertilizer, and preparation method and applications thereof - Google Patents
Rooting nutritional type microbial fertilizer, and preparation method and applications thereof Download PDFInfo
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Abstract
The invention discloses a rooting nutritional type microbial fertilizer. The rooting nutritional type microbial fertilizer is prepared from the following active ingredients in parts by weight: 50-65 parts of animal blood hydrolysate, 55-75 parts of chitosan hydrolysate, 5-15 parts of composite trace elements, 8-20 parts of potassium dihydrogen phosphate, 10-25 parts of urea, and 800-872 parts of a mixed bacterial liquid. After the ingredients are mixed evenly, the rooting nutritional type microbial fertilizer in a liquid state can be obtained; the rooting nutritional type microbial fertilizer can be used for crop cultivation. The rooting nutritional type microbial fertilizer has the beneficial effects of improving soil, increasing the quantity of soil microbes, enhancing the disease resistance and the stress resistance of crops, promoting plants to root and being beneficial for the plants to absorb nutrients; besides, the utilization rate of the fertilizer is enhanced and the use amount of the fertilizer is reduced; the rooting nutritional type microbial fertilize is in a liquid agent state and can be applied in any manner of root watering, manual spraying on leaves, drop irrigation and agricultural aircraft flying, and the fertilizer application ways are simple.
Description
Technical field
The invention belongs to microbial fertilizer technical field, be specifically related to one and take root nutritional type microbial fertilizer and its preparation method and application.
Background technology
Microbial fertilizer is that a class contains living microorganism, and farm crop can be caused to obtain the agricultural prods of the effects such as specific fertilizer effect, growth regulating effect, diseases prevention worm effect and soil organisms reparation with its vital movement and meta-bolites thereof, improving Soil structure and soil microenvironment, culture fertility, raising chemical fertilizer utilization ratio and reduction fertilizer and pesticide amount of application, preventing and treating in soil continuous cropping obstacle and raising crop yield quality etc. and can play the irreplaceable effect of other fertilizer.
The root of plant can fix plant, moisture and the mineral salt be dissolved in the water and nitrogen can be absorbed again from soil, used for plant-growth, root system also can the material such as synthesizing amino acid, vegeto-alkali, organonitrogen, plant hormone, and healthy and strong flourishing root is that plant is necessary.
Summary of the invention
The object of this invention is to provide one to take root nutritional type microbial fertilizer, it can improve soil, increases the quantity of soil microorganisms, improves disease-resistant, the anti-adversity ability of fruit-vegetable plant, improves the advantages such as utilization rate of fertilizer.
To achieve these goals, the present invention adopts following technical scheme:
One is taken root nutritional type microbial fertilizer, and its activeconstituents is formed by following composition proportion by weight:
Animal blood hydrolyzed solution 50-65 part, chitosan hydrolyzate liquid 55-75 part, composite trace element 5-15 part, potassium primary phosphate 8-20 part, urea 10-25 part, mixed bacteria liquid 800-872 part;
Described mixed bacteria liquid prepares by the following method:
(1) by bacillus amyloliquefaciens bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50h at 35-38 DEG C, obtains bacillus amyloliquefaciens bacterium liquid; Consisting of of described fermented liquid: 10% soyflour, 3% starch, 6% molasses, surplus is water, by weight percentage;
(2) by Bacillus subtilis strain with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50h at 35-38 DEG C, obtains subtilis bacterium liquid; Consisting of of described fermented liquid: glucose 6%, soyflour 5%, dipotassium hydrogen phosphate 1%, yeast extract paste 1%, magnesium sulfate 0.03%, calcium carbonate 0.5%, surplus is water, by weight percentage;
(3) by Bacillus licheniformis kind with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50h at 35-38 DEG C, obtains Bacillus licheniformis liquid; Consisting of of described fermented liquid: W-Gum 10%, glucose 6%, molasses 15%, surplus is water, by weight percentage;
(4) by bacillus thuringiensis bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50h at 35-38 DEG C, obtains bacillus thuringiensis bacterium liquid; Consisting of of described fermented liquid: potassium hydroxide 4%, phosphoric acid 3%, urea 2%, extractum carnis 1%, molasses 15%, magnesium sulfate 0.1%, zinc sulfate 0.1%, surplus is water, by weight percentage;
(5) by colloid bacillus cereus bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50h at 35-38 DEG C, obtains colloid bacillus cereus bacterium liquid; Consisting of of described fermented liquid: W-Gum 10%, peptone 0.5%, glucose 2%, extractum carnis 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.03%, calcium carbonate 0.5%, surplus is water, by weight percentage;
(6) bacillus amyloliquefaciens bacterium liquid by volume: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: the ratio of colloid bacillus cereus bacterium liquid=5-1:2-1:1:1:1, by above-mentioned five kinds of bacterium liquid mixing mixing, obtains mixed bacteria liquid;
Described chitosan hydrolyzate liquid prepares by the following method: get 45-55kg chitosan, adds 865-835kg water, and mixing, is warming up to 95-100 DEG C, then adds phosphatase 79 0-110kg, fully stirs and makes it mixing, obtain chitosan hydrolyzate liquid;
The preparation of described animal blood hydrolyzed solution: get not solidified animal blood, be warming up to 75-80 DEG C, adds animal blood lytic enzyme, hydrolysis 8-12h; Be warming up to 120 DEG C after hydrolysis, keep 1h with the enzyme that fully goes out; Go out after enzyme, be cooled to normal temperature, obtain animal blood hydrolyzed solution; The consumption of animal blood lytic enzyme is the enzyme of 1000kg animal blood 3kg.
Described composite trace element, be by etc. the following substances of quality mix: ferrous sulfate, boric acid, manganous sulfate, copper sulfate, zinc sulfate, ammonium molybdate.
Above-mentioned involved fermented liquid, all at 121 DEG C, sterilizing re-used after 2 hours.
Aerobic fermentation 48h in the preparation of preferred mixed bacteria liquid.
Preferred mixed bacteria liquid is bacillus amyloliquefaciens bacterium liquid by volume: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: colloid bacillus cereus bacterium liquid=5:2:1:1:1.
Preferred one is taken root nutritional type microbial fertilizer, and its activeconstituents is formed by following composition proportion by weight: animal blood hydrolyzed solution 55-60 part, chitosan hydrolyzate liquid 60-70 part, composite trace element 8-12 part, potassium primary phosphate 10-18 part, saltpetre 15-20 part, mixed bacteria liquid 830-850 part.
Preferred one is taken root nutritional type microbial fertilizer, and its activeconstituents is formed by following composition proportion by weight: animal blood hydrolyzed solution 58 parts, chitosan hydrolyzate liquid 75 parts, composite trace element 10 parts, potassium primary phosphate 15 parts, 18 parts, saltpetre, mixed bacteria liquid 840 parts.
Above-mentioned nutritional type microbial fertilizer of taking root, formulation is liquid.
The preparation method of above-mentioned nutritional type microbial fertilizer of taking root, gets each component, mixing mixing.
The application of above-mentioned nutritional type microbial fertilizer of taking root, the application of described fertilizer in arviculture.
Preferred farm crop are summer squash, capsicum, cucumber, Chinese cabbage rape, tomato, eggplant.
The bacillus amyloliquefaciens that the present invention is used, subtilis, Bacillus licheniformis, bacillus thuringiensis, colloid bacillus cereus is existing conventional bacterial classification in prior art, can conventional market purchase obtain, the present invention's bacterial classification used is all purchased from Chinese Academy of Agricultural Sciences's agricultural Microbiological Culture Collection administrative center (public sale), wherein, bacillus amyloliquefaciens be numbered ACCC10167, the numbering ACCC04177 of subtilis, Bacillus licheniformis be numbered ACCC02936, bacillus thuringiensis be numbered ACCC11075, colloid bacillus cereus be numbered ACCC10013.
Component in fermented liquid involved in the present invention is existing conventional constituents in prior art, all conventionally prepares, such as, and soyflour, W-Gum, molasses, yeast extract paste, extractum carnis etc.
The present invention's chitosan used is purchased from Weifang Ke Hai chitin company limited, and animal blood lytic enzyme is purchased from Pangbo Bioengineering Co Ltd, Nanning.
Beneficial effect of the present invention: improvement soil, increase the quantity of soil microorganisms, improve resistance against diseases and the anti-adversity ability of farm crop, the existence of microorganism improves the utilization ratio of fertilizer, decreases the usage quantity of fertilizer, facilitates plant establishment, be beneficial to the absorption of nutrient, nutritional type microbial fertilizer of taking root of the present invention is liquid dosage form, and can fill with root punching and execute and artificial foliage-spray and drip irrigation and Aircraft equalization, fertilization mode is simple.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
Embodiment 1
To take root the composition of nutritional type microbial fertilizer: animal blood hydrolyzed solution 50kg, chitosan hydrolyzate liquid 55kg, composite trace element 5kg, potassium primary phosphate 8kg, urea 10kg, mixed bacteria liquid 872kg.
Prepare mixed bacteria liquid:
(1) be equipped with in the fermentor tank of fermented liquid by bacillus amyloliquefaciens bacterial classification with the inoculum size access that volume ratio is 0.5%, aerobic fermentation 48h at 38 DEG C, obtains bacillus amyloliquefaciens bacterium liquid; Consisting of of described fermented liquid: 10kg soyflour, 3kg starch, 6kg molasses, surplus is water;
(2) be equipped with in the fermentor tank of fermented liquid by Bacillus subtilis strain with the inoculum size access that volume ratio is 0.4%, aerobic fermentation 50h at 38 DEG C, obtains subtilis bacterium liquid; Consisting of of described fermented liquid: glucose 6kg, soyflour 5kg, dipotassium hydrogen phosphate 1kg, yeast extract paste 1kg, magnesium sulfate 0.03kg, calcium carbonate 0.5kg, surplus is water;
(3) be equipped with in the fermentor tank of fermented liquid by Bacillus licheniformis kind with the inoculum size access that volume ratio is 0.3%, aerobic fermentation 45h at 35 DEG C, obtains Bacillus licheniformis liquid; Consisting of of described fermented liquid: W-Gum 10kg, glucose 6kg, molasses 15kg, surplus is water;
(4) be equipped with in the fermentor tank of fermented liquid by bacillus thuringiensis bacterial classification with the inoculum size access that volume ratio is 0.5%, aerobic fermentation 48h at 37 DEG C, obtains bacillus thuringiensis bacterium liquid; Consisting of of described fermented liquid: potassium hydroxide 4kg, phosphoric acid 3kg, urea 2kg, extractum carnis 1kg, molasses 15kg, magnesium sulfate 0.1kg, zinc sulfate 0.1kg, surplus is water;
(5) be equipped with in the fermentor tank of fermented liquid by colloid bacillus cereus bacterial classification with the inoculum size access that volume ratio is 0.5%, aerobic fermentation 48h at 35 DEG C, obtains colloid bacillus cereus bacterium liquid; Consisting of of described fermented liquid: W-Gum 10kg, peptone 0.5kg, glucose 2kg, extractum carnis 1kg, dipotassium hydrogen phosphate 0.5kg, magnesium sulfate 0.03kg, calcium carbonate 0.5kg, surplus is water;
(6) bacillus amyloliquefaciens bacterium liquid by volume: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: the ratio of colloid bacillus cereus bacterium liquid=5:2:1:1:1, by above-mentioned five kinds of bacterium liquid mixing mixing, obtains mixed bacteria liquid.
Prepare chitosan hydrolyzate liquid: get 45kg chitosan (purchased from Weifang Ke Hai chitin company limited) and put into reaction vessel, add 865kg water, mixing, is warming up to 96 DEG C, then adds phosphatase 79 0kg, fully stirs 2h and makes it mixing, obtain chitosan hydrolyzate liquid.
Prepared by animal blood hydrolyzed solution: get not solidified ox blood 50kg and put into fermentor tank, be warming up to 76 DEG C, add 0.15kg animal blood lytic enzyme (purchased from Nanjing Pang Bo biotechnology company limited), and hydrolysis 8h, ceaselessly stirs in hydrolytic process; Be warming up to 120 DEG C after hydrolysis, keep 1h with the enzyme that fully goes out; Go out after enzyme, be cooled to normal temperature, obtain animal blood hydrolyzed solution.
Prepared by composite trace element, component mixes.
To take root the preparation of nutritional type microbial fertilizer: each component is mixed and obtains product.
Contrast common fertilizer and the effect of nutritional type microbial fertilizer to cucumber of taking root:
Random selecting growing way same or analogous cucumber test group and each 1 mu of cucumber control group, use 5kg to test group after survival after transplant to take root nutritional type microbial fertilizer, punching is executed, comparatively use 5kg amino acid fertilizer (Liquid amino acid purchased from Shandong Hisoon biochemistry corporation,Ltd. is fertile), punching is executed, and the opportunity of fertilising is also after survival after transplant.
Result: apply fertilizer after 10 days, the growing way of test group cucumber is obviously better than control group, is embodied in: plant growing way is good, sick without yellow point, without small and weak seedling, bloom many.
Embodiment 2
To take root the composition of nutritional type microbial fertilizer: animal blood hydrolyzed solution 65kg, chitosan hydrolyzate liquid 75kg, composite trace element 15kg, potassium primary phosphate 20kg, urea 25kg, mixed bacteria liquid 800kg.
Prepare mixed bacteria liquid, prepare chitosan hydrolyzate liquid, prepare animal blood hydrolyzed solution, the preparation of composite trace element, fertilizer is with embodiment 1.
Embodiment 3
To take root the composition of nutritional type microbial fertilizer: animal blood hydrolyzed solution 58kg, chitosan hydrolyzate liquid 75kg, composite trace element 10kg, potassium primary phosphate 15kg, urea 18kg, mixed bacteria liquid 840kg.
Prepare mixed bacteria liquid, prepare chitosan hydrolyzate liquid, prepared by animal blood hydrolyzed solution, the preparation method of fertilizer is with embodiment 1.
Above-mentioned, although in conjunction with embodiment, invention has been described; but not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various amendment or distortion that creative work can make still within protection scope of the present invention.
Claims (10)
1. take root a nutritional type microbial fertilizer, it is characterized in that, its activeconstituents is formed by following composition proportion by weight:
Animal blood hydrolyzed solution 50-65 part, chitosan hydrolyzate liquid 55-75 part, composite trace element 5-15 part, potassium primary phosphate 8-20 part, urea 10-25 part, mixed bacteria liquid 800-872 part;
Described mixed bacteria liquid prepares by the following method:
(1) by bacillus amyloliquefaciens bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid,
Aerobic fermentation 45-50h at 35-38 DEG C, obtains bacillus amyloliquefaciens bacterium liquid; Consisting of of described fermented liquid: 10% soyflour, 3% starch, 6% molasses, surplus is water, by weight percentage;
(2) by Bacillus subtilis strain with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid,
Aerobic fermentation 45-50h at 35-38 DEG C, obtains subtilis bacterium liquid; Consisting of of described fermented liquid: glucose 6%,
Soyflour 5%, dipotassium hydrogen phosphate 1%, yeast extract paste 1%, magnesium sulfate 0.03%, calcium carbonate 0.5%, surplus is water, by weight percentage;
(3) by Bacillus licheniformis kind with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid,
Aerobic fermentation 45-50h at 35-38 DEG C, obtains Bacillus licheniformis liquid; Consisting of of described fermented liquid: W-Gum 10%, glucose 6%, molasses 15%, surplus is water, by weight percentage;
(4) by bacillus thuringiensis bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid,
Aerobic fermentation 45-50h at 35-38 DEG C, obtains bacillus thuringiensis bacterium liquid; Consisting of of described fermented liquid: potassium hydroxide 4%, phosphoric acid 3%, urea 2%, extractum carnis 1%, molasses 15%, magnesium sulfate 0.1%, zinc sulfate 0.1%, surplus is water, by weight percentage;
(5) by colloid bacillus cereus bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid,
Aerobic fermentation 45-50h at 35-38 DEG C, obtains colloid bacillus cereus bacterium liquid; Consisting of of described fermented liquid: W-Gum 10%, peptone 0.5%, glucose 2%, extractum carnis 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.03%, calcium carbonate 0.5%, surplus is water, by weight percentage;
(6) bacillus amyloliquefaciens bacterium liquid by volume: subtilis bacterium liquid: Bacillus licheniformis liquid: Su Yun gold gemma
Bacillus bacterium liquid: colloid bacillus cereus bacterium liquid=5-1:2-1:1:1:1, mixing mixing, obtains mixed bacteria liquid; The preparation of described chitosan hydrolyzate liquid: get 45-55kg chitosan, adds 865-835kg water, and mixing, is warming up to 95-100 DEG C, then adds phosphatase 79 0-110kg, fully stirs and makes it mixing, obtain chitosan hydrolyzate liquid;
The preparation of described animal blood hydrolyzed solution: get not solidified animal blood, be warming up to 75-80 DEG C, adds animal blood lytic enzyme, hydrolysis 8-12h; Be warming up to 120 DEG C after hydrolysis, keep 1h with the enzyme that fully goes out; Go out after enzyme, be cooled to normal temperature, obtain animal blood hydrolyzed solution; The consumption of animal blood lytic enzyme is the enzyme of 1000kg animal blood 3kg.
2. the nutritional type microbial fertilizer of taking root as described in claim 1, is characterized in that, described composite trace element, is that boric acid, manganous sulfate, copper sulfate, zinc sulfate, ammonium molybdate mixes by waiting quality acid ferrous.
3. the nutritional type microbial fertilizer of taking root as described in claim 1, is characterized in that, the sterilizing at 121 DEG C of described fermented liquid used after 2 hours.
4. the nutritional type microbial fertilizer of taking root as described in claim 1, is characterized in that, aerobic fermentation 48h in the preparation of described mixed bacteria liquid.
5. the nutritional type microbial fertilizer of taking root as described in claim 1, it is characterized in that, described mixed bacteria liquid is bacillus amyloliquefaciens bacterium liquid by volume: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: colloid bacillus cereus bacterium liquid=5:2:1:1:1.
6. the nutritional type microbial fertilizer of taking root as described in claim 1, it is characterized in that, described nutritional type microbial fertilizer of taking root, its activeconstituents is formed by following composition proportion by weight: animal blood hydrolyzed solution 55-60 part, chitosan hydrolyzate liquid 60-70 part, composite trace element 8-12 part, potassium primary phosphate 10-18 part, saltpetre 15-20 part, mixed bacteria liquid 830-850 part.
7. the nutritional type microbial fertilizer of taking root as described in claim 6, it is characterized in that, described nutritional type microbial fertilizer of taking root, its activeconstituents is formed by following composition proportion by weight: animal blood hydrolyzed solution 58 parts, chitosan hydrolyzate liquid 75 parts, composite trace element 10 parts, potassium primary phosphate 15 parts, 18 parts, saltpetre, mixed bacteria liquid 840 parts.
8. the preparation method of the nutritional type microbial fertilizer of taking root as described in claim as any in claim 1-7, is characterized in that, get each component, mixing mixing.
9. the application of the nutritional type microbial fertilizer of taking root as described in claim as any in claim 1-8, is characterized in that, the application of described fertilizer in arviculture.
10. the application of the nutritional type microbial fertilizer of taking root as described in claim 9, is characterized in that, described farm crop are summer squash, capsicum, cucumber, Chinese cabbage, rape, tomato, eggplant.
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| CN105967961A (en) * | 2016-02-25 | 2016-09-28 | 马鞍山市全润农业科技有限公司 | Rooting-promoting nutritional modified-Chinese-chestnut-protein-coated slow-release fertilizer |
| CN107129939A (en) * | 2016-02-29 | 2017-09-05 | 株式会社可伶格林 | Produce the microorganism of chitosan catabolic enzyme and utilize its plant culture composition and cultural method |
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| CN107721692A (en) * | 2017-12-05 | 2018-02-23 | 龚贤飞 | A kind of liquid fertilizer |
| CN111960902A (en) * | 2020-09-09 | 2020-11-20 | 新智创科技有限公司 | Rooting type microbial agent and preparation method thereof |
| CN112655719A (en) * | 2021-01-22 | 2021-04-16 | 东北农业大学 | Microbial preparation for promoting rice rooting and preparation method and application thereof |
| CN112876304A (en) * | 2021-03-12 | 2021-06-01 | 张贺亮 | Composite microbial fertilizer and preparation method and application thereof |
| CN113248329B (en) * | 2021-07-02 | 2021-10-12 | 山东丰本生物科技股份有限公司 | Microbial fertilizer with continuous cropping resistance effect and preparation method and application thereof |
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| KR101065539B1 (en) * | 2009-04-16 | 2011-09-19 | 김철호 | Manufacturing method of compound fertilizer and compound fertilizer using animal blood |
| CN103073354A (en) * | 2013-01-29 | 2013-05-01 | 深圳市深博泰生物科技有限公司 | Special organic fertilizer and preparation method thereof |
| CN103524270A (en) * | 2013-10-30 | 2014-01-22 | 安徽万利生态园林景观有限公司 | Fruit tree special-purpose fertilizer and preparation method thereof |
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