CN106305070A - High-yield cuttage propagation method of platycodon grandiflorum - Google Patents
High-yield cuttage propagation method of platycodon grandiflorum Download PDFInfo
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- CN106305070A CN106305070A CN201610695569.3A CN201610695569A CN106305070A CN 106305070 A CN106305070 A CN 106305070A CN 201610695569 A CN201610695569 A CN 201610695569A CN 106305070 A CN106305070 A CN 106305070A
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- cutting
- substrate
- radix platycodonis
- base material
- cutting wood
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract
The invention discloses a high-yield cuttage propagation method of platycodon grandiflorum. The high-yield cuttage propagation method comprises the following steps: selecting biennial platycodon grandiflorum without diseases as a female parent and taking a lateral bud with four leaves and one heart as a cutting wood; putting the cutting wood into a rooting agent and immersing for 5min to 6min; preparing a base material: uniformly mixing remote mountain humus soil, stiff silkworm extract and organic fertilizer at the mass ratio of (40 to 50) to (3 to 5) to (7 to 8), and controlling the humidity of the base material to be 28 percent to 30 percent by utilizing honey water with the mass percent of 2 percent; filling the base material into a bag; inserting the cutting wood into the base material, wherein the depth of inserting the cutting wood into the base material accounts for 5/12 of the total length; controlling the humidity of the base material to be 28 percent to 30 percent during a cutting process; at the 10th day after inserting the cutting wood into the base material, irradiating the cutting wood for 30min by utilizing 450nm blue light and 400nm violet light at 4:00p.m. to 5:00p.m. respectively; after culturing the cutting wood in the base material for three to three and a half months, transplanting the cutting wood. According to the high-yield cuttage propagation method, the yield and quality of the obtained platycodon grandiflorum are very high.
Description
Technical field
The present invention relates to agricultural plantation technology field.It is more particularly related to the high yield cuttage of a kind of Radix Platycodonis is numerous
Culturing method.
Background technology
Radix Platycodonis, another name burden flower, Radix Platycodonis, mitral flower, ornamental flower can be made.Its root can be used as medicine, and has eliminating phlegm and stopping cough, a surname
Lung, evacuation of pus etc. act on, traditional Chinese medical science common medicine.Tender leaf can pickle into brined vegetable, and in Yanbian of China, Radix Platycodonis is the most famous Pickles food
Material.
Radix Platycodonis as medicine, eat, appreciate dual-purpose plant, the long-term demand of China about 6 × 106T, and demand has cumulative year after year
Trend, depends merely on wild Radix Platycodonis resource and can not meet demand.The seventies in last century, China starts large area artificial culture Radix Platycodonis,
Through the development of decades, the plantation of Radix Platycodonis is all faced with a problem: root rot, purple root rot (Helicobasidium mompa) and the wheel that Radix Platycodonis continuous cropping causes
Stricture of vagina disease has a strong impact on yield and the quality of Radix Platycodonis.Current manual's plantation has become the main source of Radix Platycodonis medical material, at artificial culture
Under conditions of, the control of its property of medicine and the improvement of quality become the key problem of Radix Platycodonis breeding
Summary of the invention
It is an object of the invention to solve at least the above, and the advantage that at least will be described later is provided.
It is a still further object of the present invention to provide the cuttage breeding method of a kind of Radix Platycodonis, improve productivity and the quality of Radix Platycodonis.
In order to realize according to object of the present invention and further advantage, it is provided that the high yield cottage propagation side of a kind of Radix Platycodonis
Method, comprises the following steps:
1) select life in 2 years, disease-free Radix Platycodonis as female parent, select 4 leaves lateral bud wholeheartedly as cutting, and by cutting
Put into immersion 5-6min in root-growing agent；
2) preparation substrate: remote mountains fertile soil, Bombyx Batryticatus extract and fertilizer in mass ratio 40～50:3～5:7～8 is mixed
Close uniformly, and with the hydromel that mass fraction is 2% by the humid control of substrate 28%～30%, this substrate is loaded bag
In；
3) inserting in substrate by cutting, cutting is inserted the degree of depth in substrate and is accounted for the 5/12 of its total length, and period keeps substrate
Humid control, 28%～30%, inserts the 10th day after substrate, and every other day, 4～5 use 450nm blue light in the afternoon
Cutting 30min is irradiated respectively with 400nm purple light；
4), after cutting cultivates three to three first quarter moons in substrate, transplant.
Preferably, cutting, before putting into root-growing agent, also have passed through pretreatment:
Cutting is perpendicularly fixed in container, equipped with soak in container, the 1/2 of soak submergence cutting total length, will
It is 100w that container is placed in power, and temperature is set as 10-15min in the ultrasonic equipment of 35 DEG C；Described soak by water, ethanol,
Shikimic acid and Bombyx Batryticatus extract 500:30:10:1 in mass ratio mixes.
Preferably, in 4), cutting is removed together with substrate in the lump when transplanting.
Preferably, the 7th day after transplanting starts, applies fertilizer every other month, and sowing amount is 1-1.5kg/m2.
Preferably, described fertilizer includes the component of following parts by weight:
10-12 part chitosan, 30-35 part carbamide, 5-8 part Herba Leonuri paste, 15-18 part potassium dihydrogen phosphate, 13-16 part sulphuric acid
Ammonium, 3-5 part Semen phaseoli radiati slag and 4.5 portions of microbial inoculums；
Described microbial inoculum is made up of strain and culture medium, and the mass ratio of strain and culture medium is 1:30, and described strain is hay
Bacillus cereus and Bacillus licheniformis.
Preferably, described culture medium is by the hydromel that mass fraction is 5%, mushroom slag, biogas residue fermentation material and Herba Dendrobii
Extract 40:2:3:1 in mass ratio forms.
Preferably, described root-growing agent is by naphthalene acetic acid, indolebutyric acid, vitamin B1 and water 50:20:15:100 in mass ratio
It is mixed.
The present invention at least includes following beneficial effect:
The judgment criteria of medicinal Radix Platycodonis is: root manure is big, color is white, quality consolidation, bitter in the mouth person are good, and the cutting of the present invention is carried out
2 years-2 year half of cottage propagation, i.e. can reach the quality of common 3 years raw Radix Platycodonis, substantially reduce the cultivation period of Radix Platycodonis.
Using 2 years raw Radix Platycodonis as female parent, plant bar stem is sturdy, tall and big, and in conjunction with the method for the present invention, cutting is being bloomed
Phase plant height can reach 69cm, and cutting is when becoming the plant of life in 2 years, and its inner texture is tight, and crack is tiny, cambium ring
Brown is obvious, and bitterness is obvious.The total saponin content of raw cutting in 2 years can reach more than 12.3%.
The fertilizer of the present invention, according to the upgrowth situation of Radix Platycodonis, is adjusted to and executes every other month, it is to avoid fertilizer too much causes soil to be tied
Structure changes, and affects the growth of Radix Platycodonis on the contrary, it addition, this fertilizer can effectively prevent and treat root rot, purple root rot (Helicobasidium mompa) and ring spot, does not allows
Easy infection pest and disease damage, survival rate is up to more than 98.2%.
Part is embodied by the further advantage of the present invention, target and feature by description below, and part also will be by this
Invention research and practice and be understood by the person skilled in the art.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, to make those skilled in the art with reference to description
Word can be implemented according to this.
It should be noted that experimental technique described in following embodiment, if no special instructions, it is conventional method, institute
State reagent and material, if no special instructions, the most commercially obtain.
Embodiment 1
The high yield cuttage breeding method of a kind of Radix Platycodonis, it is characterised in that comprise the following steps:
1) select life in 2 years, disease-free Radix Platycodonis as female parent, select 4 leaves lateral bud wholeheartedly as cutting, and by cutting
Put into immersion 5min in root-growing agent.
2) preparation substrate: by remote mountains fertile soil, Bombyx Batryticatus extract and the fertilizer mix homogeneously of 50:5:8 in mass ratio, and use
Mass fraction be the hydromel of 2% by the humid control of substrate 30%, this substrate is loaded in bag.
3) inserting in substrate by cutting, cutting is inserted the degree of depth in substrate and is accounted for the 5/12 of its total length, and period keeps substrate
Humid control, 30%, inserts the 10th day after substrate, and every other day, 4～5 use 450nm blue light and 400nm in the afternoon
Purple light irradiates cutting 30min respectively.
4) after cutting is cultivated three months in substrate, transplanting, connection substrate puts into hole together, and earthing continues
Cultivate.
Cultivating Radix Platycodonis cutting 500 in aforementioned manners, check after two months and take root 492, the seedling after taking root enters
Row is transplanted, and survives 491 strains, and survival rate is 98.2%, and raw cutting in 2 years gathered in the August, and average total saponin content is
12.3%.
Embodiment 2
The high yield cuttage breeding method of a kind of Radix Platycodonis, it is characterised in that comprise the following steps:
1) select life in 2 years, disease-free Radix Platycodonis as female parent, select 4 leaves lateral bud wholeheartedly as cutting, by cutting warp
Cross pretreatment:
Cutting is perpendicularly fixed in container, equipped with soak in container, the 1/2 of soak submergence cutting total length, will
It is 100w that container is placed in power, and temperature is set as 10min in the ultrasonic equipment of 35 DEG C；Described soak is by water, ethanol, Folium illicii Lanceolati
Acid and Bombyx Batryticatus extract 500:30:10:1 in mass ratio mix.Pretreated cutting is put in root-growing agent and soak
6min, described root-growing agent is mixed by naphthalene acetic acid, indolebutyric acid, vitamin B1 and water 50:20:15:100 in mass ratio.
2) preparation substrate: by remote mountains fertile soil, Bombyx Batryticatus extract and the fertilizer mix homogeneously of 40:3:7 in mass ratio, and use
Mass fraction be the hydromel of 2% by the humid control of substrate 28%, this substrate is loaded in bag.
3) inserting in substrate by cutting, cutting is inserted the degree of depth in substrate and is accounted for the 5/12 of its total length, and period keeps substrate
Humid control, 28%, inserts the 10th day after substrate, and every other day, 4～5 use 450nm blue light and 400nm in the afternoon
Purple light irradiates cutting 30min respectively；
4) after three first quarter moons are cultivated in cutting in substrate, it being transplanted together with substrate, put into hole, earthing continues
Continuous cultivation.
5) within the 7th day after transplanting, starting, apply fertilizer every other month, sowing amount is 1kg/m2.Described fertilizer bag
Include the component of following parts by weight:
10 parts of chitosans, 30 parts of carbamide, 5 parts of Herba Leonuri pastes, 15 parts of potassium dihydrogen phosphates, 13 parts of ammonium sulfate, 3 parts of Semen phaseoli radiati slags and
4.5 portions of microbial inoculums；
Described microbial inoculum is made up of strain and culture medium, and the mass ratio of strain and culture medium is 1:30, and described strain is hay
Bacillus cereus and Bacillus licheniformis.Described culture medium is by the hydromel that mass fraction is 5%, mushroom slag, biogas residue fermentation material and ferrum
Skin Herba Dendrobii extract 40:2:3:1 in mass ratio forms.
Cultivating Radix Platycodonis cutting 500 in aforementioned manners, check after two months and take root 498, the seedling after taking root enters
Row is transplanted, and survives 495 strains, and survival rate is 99%, and raw cutting in 2 years gathered in the August, and average total saponin content is 13.2%.
Embodiment 3
The high yield cuttage breeding method of a kind of Radix Platycodonis, it is characterised in that comprise the following steps:
1) select life in 2 years, disease-free Radix Platycodonis as female parent, select 4 leaves lateral bud wholeheartedly as cutting, and by cutting
Put into immersion 5min in root-growing agent；
2) preparation substrate: by remote mountains fertile soil, Bombyx Batryticatus extract and the fertilizer mix homogeneously of 45:4:7 in mass ratio, and use
Mass fraction be the hydromel of 2% by the humid control of substrate 29%, this substrate is loaded in bag；
3) inserting in substrate by cutting, cutting is inserted the degree of depth in substrate and is accounted for the 5/12 of its total length, and period keeps substrate
Humid control, 29%, inserts the 10th day after substrate, and every other day, 4～5 use 450nm blue light and 400nm in the afternoon
Purple light irradiates cutting 30min respectively；
4) after cutting is cultivated three months in substrate, it being transplanted together with substrate, put into hole, earthing continues
Cultivate.
5) within the 7th day after transplanting, starting, apply fertilizer every other month, sowing amount is 1.5kg/m2。
Described fertilizer includes the component of following parts by weight:
11 parts of chitosans, 35 parts of carbamide, 8 parts of Herba Leonuri pastes, 18 parts of potassium dihydrogen phosphates, 16 parts of ammonium sulfate, 5 parts of Semen phaseoli radiati slags and
4.5 portions of microbial inoculums；
Described microbial inoculum is made up of strain and culture medium, and the mass ratio of strain and culture medium is 1:30, and described strain is hay
Bacillus cereus and Bacillus licheniformis.Described culture medium is by the hydromel that mass fraction is 5%, mushroom slag, biogas residue fermentation material and ferrum
Skin Herba Dendrobii extract 40:2:3:1 in mass ratio forms.
Cultivating Radix Platycodonis cutting 500 in aforementioned manners, check after two months and take root 493, the seedling after taking root enters
Row is transplanted, and survives 493 strains, and survival rate is 98.6%, and raw cutting in 2 years gathered in the August, and average total saponin content is
12.5%.
Although embodiment of the present invention are disclosed as above, but it is not restricted in description and embodiment listed
Using, it can be applied to various applicable the field of the invention completely, for those skilled in the art, and can be easily
Realizing other amendment, therefore under the general concept limited without departing substantially from claim and equivalency range, the present invention does not limit
In specific details with shown here as the embodiment with description.
Claims (7)
1. the high yield cuttage breeding method of a Radix Platycodonis, it is characterised in that comprise the following steps:
1) select life in 2 years, disease-free Radix Platycodonis as female parent, select 4 leaves lateral bud wholeheartedly as cutting, and cutting is put into
Root-growing agent soaks 5-6min；
2) preparation substrate: by equal in mass ratio 40～50:3～5:7～8 mixing of remote mountains fertile soil, Bombyx Batryticatus extract and fertilizer
Even, and with the hydromel that mass fraction is 2% by the humid control of substrate 28%～30%, this substrate is loaded in bag；
3) inserting in substrate by cutting, cutting is inserted the degree of depth in substrate and is accounted for the 5/12 of its total length, and period keeps soil humidity
Control 28%～30%, insert the 10th day after substrate, every other day, in the afternoon 4～5 use 450nm blue lights and
400nm purple light irradiates cutting 30min respectively；
4), after cutting cultivates three to three first quarter moons in substrate, transplant.
2. the high yield cuttage breeding method of Radix Platycodonis as claimed in claim 1, it is characterised in that cutting before putting into root-growing agent,
Also have passed through pretreatment:
Cutting being perpendicularly fixed in container, equipped with soak in container, the 1/2 of soak submergence cutting total length, by container
Being placed in power is 100w, and temperature is set as 10-15min in the ultrasonic equipment of 35 DEG C；Described soak is by water, ethanol, Folium illicii Lanceolati
Acid and Bombyx Batryticatus extract 500:30:10:1 in mass ratio mix.
3. the high yield cuttage breeding method of Radix Platycodonis as claimed in claim 1, it is characterised in that 4) in cutting when transplanting together with
Substrate is removed in the lump.
4. the high yield cuttage breeding method of Radix Platycodonis as claimed in claim 3, it is characterised in that within the 7th day after transplanting, start,
Applying fertilizer every other month, sowing amount is 1-1.5kg/m2。
5. the high yield cuttage breeding method of Radix Platycodonis as claimed in claim 4, it is characterised in that described fertilizer includes following heavy
The component of amount number:
10-12 part chitosan, 30-35 part carbamide, 5-8 part Herba Leonuri paste, 15-18 part potassium dihydrogen phosphate, 13-16 part ammonium sulfate,
3-5 part Semen phaseoli radiati slag and 4.5 portions of microbial inoculums；
Described microbial inoculum is made up of strain and culture medium, and the mass ratio of strain and culture medium is 1:30, and described strain is hay spore
Bacillus and Bacillus licheniformis.
6. the high yield cuttage breeding method of Radix Platycodonis as claimed in claim 5, it is characterised in that described culture medium is by mass fraction
It is hydromel, mushroom slag, biogas residue fermentation material and the Herba Dendrobii extract 40:2:3:1 in mass ratio composition of 5%.
7. the high yield cuttage breeding method of Radix Platycodonis as claimed in claim 1, it is characterised in that described root-growing agent by naphthalene acetic acid,
Indolebutyric acid, vitamin B1 and water 50:20:15:100 in mass ratio is mixed.
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| CN201610695569.3A CN106305070A (en) | 2016-08-18 | 2016-08-18 | High-yield cuttage propagation method of platycodon grandiflorum |
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Cited By (5)
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| CN107231998A (en) * | 2017-05-27 | 2017-10-10 | 马鞍山市永辉园林绿化有限公司 | A kind of cuttage and seedling culture method of trident maple |
| CN107484534A (en) * | 2017-09-26 | 2017-12-19 | 巫溪县瑞雪药材种植有限责任公司 | A kind of method for culturing seedlings of Radix Codonopsis high yield and preventing disease and pest |
| CN108566863A (en) * | 2018-06-27 | 2018-09-25 | 全椒县城西畜牧养殖专业合作社 | A kind of implantation methods improving winter jujube diseases and insect pests resistance |
| CN109105139A (en) * | 2018-07-28 | 2019-01-01 | 贵州金州农耕生态农业股份有限公司 | A kind of grape cuttage and seedling culture method |
| CN109220270A (en) * | 2018-11-06 | 2019-01-18 | 北京林业大学 | A kind of Magnolia wufengensis softwood cutting propagation method based on optimal hormone combination |
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| CN109105139A (en) * | 2018-07-28 | 2019-01-01 | 贵州金州农耕生态农业股份有限公司 | A kind of grape cuttage and seedling culture method |
| CN109220270A (en) * | 2018-11-06 | 2019-01-18 | 北京林业大学 | A kind of Magnolia wufengensis softwood cutting propagation method based on optimal hormone combination |
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Application publication date: 20170111 |