CN103951500B - A kind of swollen fruit type microbial fertilizer and its preparation method and application - Google Patents
A kind of swollen fruit type microbial fertilizer and its preparation method and application Download PDFInfo
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- CN103951500B CN103951500B CN201410136476.8A CN201410136476A CN103951500B CN 103951500 B CN103951500 B CN 103951500B CN 201410136476 A CN201410136476 A CN 201410136476A CN 103951500 B CN103951500 B CN 103951500B
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 88
- 235000013399 edible fruits Nutrition 0.000 title claims abstract description 46
- 230000000813 microbial effect Effects 0.000 title claims abstract description 41
- 238000002360 preparation method Methods 0.000 title claims description 15
- 239000007788 liquid Substances 0.000 claims abstract description 108
- 239000008280 blood Substances 0.000 claims abstract description 34
- 210000004369 blood Anatomy 0.000 claims abstract description 34
- 241000894006 Bacteria Species 0.000 claims abstract description 28
- 229920001661 Chitosan Polymers 0.000 claims abstract description 25
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000011573 trace mineral Substances 0.000 claims abstract description 17
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 17
- 239000002131 composite material Substances 0.000 claims abstract description 16
- 239000000203 mixture Substances 0.000 claims abstract description 15
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 15
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 11
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 11
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 11
- 239000010452 phosphate Substances 0.000 claims abstract description 11
- 239000011591 potassium Substances 0.000 claims abstract description 11
- 239000004323 potassium nitrate Substances 0.000 claims abstract description 11
- 235000010333 potassium nitrate Nutrition 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 18
- 238000002156 mixing Methods 0.000 claims description 16
- 238000010564 aerobic fermentation Methods 0.000 claims description 15
- 239000002054 inoculum Substances 0.000 claims description 15
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 12
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 11
- 241000193755 Bacillus cereus Species 0.000 claims description 11
- 241000194108 Bacillus licheniformis Species 0.000 claims description 11
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 11
- 230000001580 bacterial effect Effects 0.000 claims description 11
- 239000000084 colloidal system Substances 0.000 claims description 11
- 238000010792 warming Methods 0.000 claims description 11
- 235000013379 molasses Nutrition 0.000 claims description 10
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 9
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 9
- 239000008103 glucose Substances 0.000 claims description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 9
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 6
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 6
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 6
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 6
- 229960001763 zinc sulfate Drugs 0.000 claims description 6
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 5
- 230000007062 hydrolysis Effects 0.000 claims description 5
- 238000006460 hydrolysis reaction Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 229940041514 candida albicans extract Drugs 0.000 claims description 4
- 239000012138 yeast extract Substances 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims description 3
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 3
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 3
- 239000011609 ammonium molybdate Substances 0.000 claims description 3
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 3
- 229940010552 ammonium molybdate Drugs 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 3
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 3
- 229960000355 copper sulfate Drugs 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 2
- 230000012010 growth Effects 0.000 abstract description 11
- 239000002689 soil Substances 0.000 abstract description 8
- 230000002262 irrigation Effects 0.000 abstract description 6
- 238000003973 irrigation Methods 0.000 abstract description 6
- 238000004080 punching Methods 0.000 abstract description 6
- 230000009286 beneficial effect Effects 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 3
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 230000007423 decrease Effects 0.000 abstract description 2
- 230000004720 fertilization Effects 0.000 abstract description 2
- 230000006872 improvement Effects 0.000 abstract description 2
- 239000008297 liquid dosage form Substances 0.000 abstract description 2
- 239000007921 spray Substances 0.000 abstract description 2
- 241000208293 Capsicum Species 0.000 description 36
- 235000002566 Capsicum Nutrition 0.000 description 36
- 239000001390 capsicum minimum Substances 0.000 description 21
- 230000008676 import Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 3
- 229920002101 Chitin Polymers 0.000 description 2
- 230000005078 fruit development Effects 0.000 description 2
- 230000003301 hydrolyzing effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 1
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 1
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 240000001980 Cucurbita pepo Species 0.000 description 1
- 235000009852 Cucurbita pepo Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002439 hemostatic effect Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
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- Fertilizers (AREA)
Abstract
The invention discloses a kind of swollen fruit type microbial fertilizer, it is made up of the component of following weight part: animal blood hydrolyzed solution 64-70 part, chitosan hydrolyzate liquid 70-80 part, composite trace element 5-15 part, potassium primary phosphate 55-75 part, saltpetre 85-105 part, mixed bacteria liquid 655-721 part; By each component, mix and obtain this fertilizer of liquid-type, the application of this fertilizer in arviculture, beneficial effect of the present invention: improvement soil, increase the quantity of soil microorganisms, improve resistance against diseases and the anti-adversity ability of farm crop, improve the utilization ratio of fertilizer, decrease the usage quantity of fertilizer, facilitate the growth of fruit, avoid shedding, improve the mouthfeel of fruit, be beneficial to the absorption of nutrient, this fertilizer is liquid dosage form, and can fill with root punching and execute and artificial foliage-spray and drip irrigation and Aircraft equalization, fertilization mode is simple.
Description
Technical field
The invention belongs to microbial fertilizer technical field, be specifically related to a kind of swollen fruit type microbial fertilizer and its preparation method and application.Background technology
Microbial fertilizer is that a class contains living microorganism, and farm crop can be caused to obtain the agriculture data product of the effects such as specific fertilizer effect, growth regulating effect, diseases prevention worm effect and soil organisms reparation with its vital movement and meta-bolites thereof, improving Soil structure and soil microenvironment, culture fertility, raising chemical fertilizer utilization ratio and reduction fertilizer and pesticide amount of application, preventing and treating in soil continuous cropping obstacle and raising crop yield quality etc. and can play the irreplaceable effect of other fertilizer.
Fruit needs a large amount of nutrients in the process of growing, and the demand of fertilizer and the difference to some extent of the fertilizer ratio required for the plant strain growth phase, for promoting that swollen fruit needs to promote that fruit development can improve again the fertilizer of disease resistance.
Summary of the invention
Object of the present invention is exactly to solve the problem, and provides a kind of swollen fruit type microbial fertilizer and its preparation method and application, and it has promotion fruit development, avoids shedding, improves the advantages such as the mouthfeel of fruit.
To achieve these goals, the present invention adopts following technical scheme:
A kind of swollen fruit type microbial fertilizer, its activeconstituents is formed by following composition proportion by weight: animal blood hydrolyzed solution 64-70 part, chitosan hydrolyzate liquid 70-80 part, composite trace element 5-15 part, potassium primary phosphate 55-75 part, saltpetre 85-105 part, mixed bacteria liquid 655-721 part;
Described mixed bacteria liquid prepares by the following method:
(1) by bacillus amyloliquefaciens bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50 hour at 35-38 DEG C, obtains bacillus amyloliquefaciens bacterium liquid; Consisting of of described fermented liquid: 10% soyflour, 3% starch, 6% molasses, surplus is water, by weight percentage;
(2) by Bacillus subtilis strain with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50 hour at 35-38 DEG C, obtains subtilis bacterium liquid; Consisting of of described fermented liquid: glucose 6%, soyflour 5%, dipotassium hydrogen phosphate 1%, yeast extract paste 1%, magnesium sulfate 0.03%, calcium carbonate 0.5%, surplus is water, by weight percentage;
(3) by Bacillus licheniformis strain with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50 hour at 35-38 DEG C, obtains Bacillus licheniformis liquid; Consisting of of described fermented liquid: W-Gum 10%, glucose 6%, molasses 15%, surplus is water, by weight percentage;
(4) by bacillus thuringiensis bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50 hour at 35-38 DEG C, obtains bacillus thuringiensis bacterium liquid; Consisting of of described fermented liquid: potassium hydroxide 4%, phosphoric acid 3%, urea 2%, extractum carnis 1%, molasses 15%, magnesium sulfate 0.1%, zinc sulfate 0.1%, surplus is water, by weight percentage;
(5) by colloid bacillus cereus bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50 hour at 35-38 DEG C, obtains colloid bacillus cereus bacterium liquid; Consisting of of described fermented liquid: W-Gum 10%, peptone 0.5%, glucose 2%, extractum carnis 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.03%, calcium carbonate 0.5%, surplus is water, by weight percentage;
(6) bacillus amyloliquefaciens bacterium liquid by volume: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: the ratio of colloid bacillus cereus bacterium liquid=5-1:2-1:1:1:1, by above-mentioned five kinds of bacterium liquid mixing mixing, obtains mixed bacteria liquid;
Described chitosan hydrolyzate liquid prepares by the following method: get 45-55kg chitosan, adds 865-835kg water, and mixing, is warming up to 95-100 DEG C, then adds phosphatase 79 0-110kg, fully stirs and makes it mixing, obtain chitosan hydrolyzate liquid;
Described animal blood hydrolyzed solution preparation: get not solidified animal blood, be warming up to 65-70 DEG C, add animal blood lytic enzyme, hydrolysis 8-12 hour, ceaselessly stirs in case Hemostatic Oral Liquid solidifies in hydrolytic process; Be warming up to 100 DEG C after hydrolysis, keep 1 hour with the enzyme that fully goes out; Go out after enzyme, be cooled to normal temperature, obtain animal blood hydrolyzed solution; The consumption of animal blood lytic enzyme is the enzyme of every 1000kg animal blood 3kg;
Described composite trace element, be by etc. the following substances of quality mix: ferrous sulfate, boric acid, manganous sulfate, copper sulfate, zinc sulfate, ammonium molybdate.
Above-mentioned involved fermented liquid, all at 121 DEG C, sterilizing re-used after 2 hours.
The swollen fruit type microbial fertilizer of preferred one, its activeconstituents is formed by following composition proportion by weight: animal blood hydrolyzed solution 65-68 part, chitosan hydrolyzate liquid 73-77 part, composite trace element 8-12 part, potassium primary phosphate 60-68 part, saltpetre 93-100 part, mixed bacteria liquid 680-700 part.
Preferred swollen fruit type microbial fertilizer, its activeconstituents is formed by following composition proportion by weight: animal blood hydrolyzed solution 67 parts, chitosan hydrolyzate liquid 75 parts, composite trace element 10 parts, potassium primary phosphate 64 parts, 96 parts, saltpetre, mixed bacteria liquid 690 parts.
Above-mentioned swollen fruit type microbial fertilizer, its formulation is liquid.
The preparation method of above-mentioned swollen fruit type microbial fertilizer, gets each component, and mixing mixing, to obtain final product.
The application of above-mentioned swollen fruit type microbial fertilizer in arviculture, preferred farm crop are summer squash, capsicum, cucumber, Chinese cabbage rape, tomato, eggplant.
The bacillus amyloliquefaciens that the present invention is used, subtilis, Bacillus licheniformis, bacillus thuringiensis, colloid bacillus cereus is existing conventional bacterial classification in prior art, can conventional market purchase obtain, the present invention's bacterial classification used is all purchased from Chinese Academy of Agricultural Sciences's agricultural Microbiological Culture Collection administrative center (public sale), wherein, bacillus amyloliquefaciens be numbered ACCC10167, the numbering ACCC04177 of subtilis, Bacillus licheniformis be numbered ACCC02936, bacillus thuringiensis be numbered ACCC11075, colloid bacillus cereus be numbered ACCC10013.
Component in fermented liquid involved in the present invention is existing conventional constituents in prior art, all conventionally prepares, such as, and soyflour, W-Gum, molasses, yeast extract paste, extractum carnis etc.
Animal blood lytic enzyme is purchased from Pangbo Bioengineering Co Ltd, Nanning, and chitosan is purchased from Weifang Ke Hai chitin company limited.
Beneficial effect of the present invention: improvement soil, increase the quantity of soil microorganisms, improve resistance against diseases and the anti-adversity ability of farm crop, the existence of microorganism improves the utilization ratio of fertilizer, decreases the usage quantity of fertilizer, facilitate the growth of fruit, avoid shedding, improve the mouthfeel of fruit, be beneficial to the absorption of nutrient, this fertilizer is liquid dosage form, can fill with root punching to execute and artificial foliage-spray and drip irrigation and Aircraft equalization, fertilization mode is simple.
Embodiment
The invention will be further described for embodiment below.
Embodiment 1
The composition of swollen fruit type microbial fertilizer: animal blood hydrolyzed solution 64kg, chitosan hydrolyzate liquid 70kg, composite trace element 5kg, potassium primary phosphate 55kg, saltpetre 85kg, mixed bacteria liquid 721kg.
Prepare mixed bacteria liquid:
(1) be equipped with in the fermentor tank of fermented liquid by bacillus amyloliquefaciens bacterial classification with the inoculum size access that volume ratio is 0.5%, aerobic fermentation 48h at 38 DEG C, obtains bacillus amyloliquefaciens bacterium liquid; Consisting of of described fermented liquid: 10kg soyflour, 3kg starch, 6kg molasses, surplus is water;
(2) be equipped with in the fermentor tank of fermented liquid by Bacillus subtilis strain with the inoculum size access that volume ratio is 0.4%, aerobic fermentation 50h at 38 DEG C, obtains subtilis bacterium liquid; Consisting of of described fermented liquid: glucose 6kg, soyflour 5kg, dipotassium hydrogen phosphate 1kg, yeast extract paste 1kg, magnesium sulfate 0.03kg, calcium carbonate 0.5kg, surplus is water;
(3) be equipped with in the fermentor tank of fermented liquid by Bacillus licheniformis strain with the inoculum size access that volume ratio is 0.3%, aerobic fermentation 45h at 35 DEG C, obtains Bacillus licheniformis liquid; Consisting of of described fermented liquid: W-Gum 10kg, glucose 6kg, molasses 15kg, surplus is water;
(4) be equipped with in the fermentor tank of fermented liquid by bacillus thuringiensis bacterial classification with the inoculum size access that volume ratio is 0.5%, aerobic fermentation 48 hours at 37 DEG C, obtains bacillus thuringiensis bacterium liquid; Consisting of of described fermented liquid: potassium hydroxide 4kg, phosphoric acid 3kg, urea 2kg, extractum carnis 1kg, molasses 15kg, magnesium sulfate 0.1kg, zinc sulfate 0.1kg, surplus is water;
(5) be equipped with in the fermentor tank of fermented liquid by colloid bacillus cereus bacterial classification with the inoculum size access that volume ratio is 0.5%, aerobic fermentation 48 hours at 35 DEG C, obtains colloid bacillus cereus bacterium liquid; Consisting of of described fermented liquid: W-Gum 10kg, peptone 0.5kg, glucose 2kg, extractum carnis 1kg, dipotassium hydrogen phosphate 0.5kg, magnesium sulfate 0.03kg, calcium carbonate 0.5kg;
(6) bacillus amyloliquefaciens bacterium liquid by volume: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: the ratio of colloid bacillus cereus bacterium liquid=5:2:1:1:1 is by above-mentioned five kinds of bacterium liquid mixing mixing.
Prepare chitosan hydrolyzate liquid: get 45kg chitosan (purchased from Weifang Ke Hai chitin company limited), add 865kg water, mixing, is warming up to 96 DEG C, then adds phosphatase 79 0kg, fully stirs 2h and makes it mixing, obtain chitosan hydrolyzate liquid.
Prepared by animal blood hydrolyzed solution: get not solidified ox blood 100kg, be warming up to 67 DEG C, add 0.3kg animal blood lytic enzyme (purchased from Nanjing Pang Bo biotechnology company limited), be hydrolyzed 8 hours, ceaselessly stir in hydrolytic process; Be warming up to 100 DEG C after hydrolysis, keep 1 hour with the enzyme that fully goes out; Go out after enzyme, be cooled to normal temperature, obtain animal blood hydrolyzed solution.
The ferrous sulfate of the quality such as composite trace element is, boric acid, manganous sulfate, copper sulfate, zinc sulfate, ammonium molybdate mix.
The preparation of swollen fruit type microbial fertilizer: each component is mixed, obtains product.
The simultaneous test of swollen fruit type microbial fertilizer and common Water soluble fertilizer:
Random selecting length is 70 meters, is approximately the capsicum booth of 1.5 mu, is divided into east, west two portions, and Dong Peng length is 35 meters,
0.75 mu, the common Water soluble fertilizer of 10kg (wherein the massfraction of N P and K is 15%, 15%, 30%) is executed in punching.Long 35 meters of Xi Peng, 0.75 mu, with swollen fruit type microbial fertilizer 6kg, punching is executed.Random selecting 50 strain growthdevelopmental stage, the same or analogous capsicum of growing way, every strain random selecting length each one in the capsicum of 12-16cm, the length of measure the front length of fertilising, applying fertilizer latter 3 days, in triplicate.
The capsicum growth results of Xi Peng
| To apply fertilizer the mean length of front 50 capsicums | To apply fertilizer the mean length of rear 3 days 50 capsicums | Average increment |
| 15cm | 18.5cm | 3.5cm |
| 13.5cm | 16cm | 2.5cm |
| 13cm | 16.5cm | 3.5cm |
150 capsicums, three days average increment 3.1cm.
The capsicum growth results of Dong Peng
| To apply fertilizer the mean length of front 50 capsicums | To apply fertilizer the mean length of rear 3 days 50 capsicums | Average increment |
| 16cm | 17.5cm | 1.5cm |
| 14cm | 16.5cm | 2.5cm |
| 12cm | 16cm | 4cm |
150 capsicums, three days average increment 2.6cm.
Embodiment 2
The composition of swollen fruit type microbial fertilizer: the composition of swollen fruit type microbial fertilizer: animal blood hydrolyzed solution 70kg, chitosan hydrolyzate liquid 80kg, composite trace element 15kg, potassium primary phosphate 75kg, saltpetre 105kg, mixed bacteria liquid 655kg.
Prepare mixed bacteria liquid, prepare chitosan hydrolyzate liquid, prepared by animal blood hydrolyzed solution, the preparation of composite trace element, fertilizer is with embodiment 1.
The simultaneous test of swollen fruit type microbial fertilizer and import Water soluble fertilizer:
Random selecting length is 50 meters, be approximately the capsicum booth of 0.63 mu, be divided into east, west two portions, Dong Peng is 0.31 mu, use the swollen fruit type microbial fertilizer of 3kg in the mode of drip irrigation, long 0.31 mu of west section canopy, (wherein the massfraction of N P and K is for 14% to use import Water soluble fertilizer 5kg in the mode of drip irrigation, 6%, 40%).Random selecting 200 strain growthdevelopmental stage, the same or analogous capsicum of growing way, every strain random selecting gets length in the capsicum of 12-13cm each one, surveys the length of the front length of its fertilising, apply fertilizer latter 4 days and 7 days.
The capsicum growth results of Xi Peng
The capsicum growth results of Dong Peng
It can thus be appreciated that, use 200 capsicums of import Water soluble fertilizer, apply fertilizer latter 4 days average increment 3cm, apply fertilizer latter 7 days average increment 6cm, use swollen fruit type microbial fertilizer, apply fertilizer latter 4 days average increment 4cm, and apply fertilizer latter 7 days average increment 9.5cm, and the effect of swollen fruit type microbial fertilizer is better than import Water soluble fertilizer.
Embodiment 3
The composition of swollen fruit type microbial fertilizer: animal blood hydrolyzed solution 67kg, chitosan hydrolyzate liquid 75kg, composite trace element 10kg, potassium primary phosphate 64kg, saltpetre 96kg, mixed bacteria liquid 690kg.
Prepare mixed bacteria liquid, prepare chitosan hydrolyzate liquid, prepared by animal blood hydrolyzed solution, the preparation of composite trace element, fertilizer is with embodiment 1.
The simultaneous test of swollen fruit type microbial fertilizer and common Water soluble fertilizer:
Random selecting length is 60 meters, and be approximately the capsicum booth of 0.8 mu, be divided into east, west two portions, Dong Peng is 0.4 mu, the common Water soluble fertilizer of 10kg (wherein the massfraction of N P and K is 15%, 10%, 25%) is executed in punching, 0.4 mu, west section canopy, with swollen fruit type microbial fertilizer 3kg, punching is executed.Get 200 strain growthdevelopmental stages, the same or analogous capsicum of growing way at random, length in the capsicum of 13-14cm each one is got in every strain at random, surveys its fertilising front length, latter 2 days length of applying fertilizer.
The capsicum growth results of Xi Peng
The capsicum growth results of Dong Peng
| To apply fertilizer the mean length of front 200 capsicums | To apply fertilizer the mean length of rear 2 days 200 capsicums | Average increment |
| 14cm | 16cm | 2cm |
Embodiment 4
The composition of swollen fruit type microbial fertilizer: animal blood hydrolyzed solution 65kg, chitosan hydrolyzate liquid 65kg, composite trace element 9kg, potassium primary phosphate 60kg, saltpetre 93kg, mixed bacteria liquid 700kg.
Prepare mixed bacteria liquid, prepare chitosan hydrolyzate liquid, prepared by animal blood hydrolyzed solution, the preparation of composite trace element, fertilizer is with embodiment 1.
The simultaneous test of swollen fruit type microbial fertilizer and import Water soluble fertilizer:
Random selecting length is 90 meters, be approximately the capsicum booth of 1.8 mu, be divided into east, west two portions, Dong Peng length is 1.43 mu, with the mode of drip irrigation use 20kg import Water soluble fertilizer (wherein the massfraction of N P and K is for 15%, 10%, 35%), 0.37 mu, west section canopy, with swollen fruit type microbial fertilizer 3kg, drip irrigation is used.Choose 50 strain growthdevelopmental stages, the same or analogous capsicum of growing way, each one of the capsicum of random selecting length between 11-13cm, the length of measure the front length of fertilising, applying fertilizer latter 3 days, in triplicate.
The capsicum growth results of Xi Peng
| To apply fertilizer the mean length of front 50 capsicums | To apply fertilizer the mean length of rear 3 days 50 capsicums | Average increment |
| 13cm | 15.1cm | 2.1cm |
| 13cm | 17cm | 4cm |
| 12cm | 14cm | 2cm |
150 capsicums, three days average increment 2.7cm.
The capsicum growth results of Dong Peng
| To apply fertilizer the mean length of front 50 capsicums | To apply fertilizer the mean length of rear 3 days 50 capsicums | Average increment |
| 13cm | 16cm | 3cm |
| 13cm | 14.5cm | 1.5cm |
| 11cm | 14cm | 3cm |
150 capsicums, three days average increment 2.5cm.
Above-mentioned, although in conjunction with embodiment, invention has been described; but not limiting the scope of the invention; one of ordinary skill in the art should be understood that; on the basis of technical scheme of the present invention, those skilled in the art do not need to pay various amendment or distortion that creative work can make still within protection scope of the present invention.
Claims (10)
1. a swollen fruit type microbial fertilizer, is characterized in that, its activeconstituents is formed by following composition proportion by weight:
Animal blood hydrolyzed solution 64-70 part, chitosan hydrolyzate liquid 70-80 part, composite trace element 5-15 part, potassium primary phosphate 55-75 part, saltpetre 85-105 part, mixed bacteria liquid 655-721 part;
Described mixed bacteria liquid prepares by the following method:
(1) by bacillus amyloliquefaciens bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50 hour at 35-38 DEG C, obtains bacillus amyloliquefaciens bacterium liquid; Consisting of of described fermented liquid: 10% soyflour, 3% starch, 6% molasses, surplus is water, by weight percentage;
(2) by Bacillus subtilis strain with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50 hour at 35-38 DEG C, obtains subtilis bacterium liquid; Consisting of of described fermented liquid: glucose 6%, soyflour 5%, dipotassium hydrogen phosphate 1%, yeast extract paste 1%, magnesium sulfate 0.03%, calcium carbonate 0.5%, surplus is water, by weight percentage;
(3) by Bacillus licheniformis strain with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50 hour at 35-38 DEG C, obtains Bacillus licheniformis liquid; Consisting of of described fermented liquid: W-Gum 10%, glucose 6%, molasses 15%, surplus is water, by weight percentage;
(4) by bacillus thuringiensis bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50 hour at 35-38 DEG C, obtains bacillus thuringiensis bacterium liquid; Consisting of of described fermented liquid: potassium hydroxide 4%, phosphoric acid 3%, urea 2%, extractum carnis 1%, molasses 15%, magnesium sulfate 0.1%, zinc sulfate 0.1%, surplus is water, by weight percentage;
(5) by colloid bacillus cereus bacterial classification with volume ratio be 0.3%-0.5% inoculum size access be equipped with in the fermentor tank of fermented liquid, aerobic fermentation 45-50 hour at 35-38 DEG C, obtains colloid bacillus cereus bacterium liquid; Consisting of of described fermented liquid: W-Gum 10%, peptone 0.5%, glucose 2%, extractum carnis 1%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate 0.03%, calcium carbonate 0.5%, surplus is water, by weight percentage;
(6) bacillus amyloliquefaciens bacterium liquid by volume: subtilis bacterium liquid: Bacillus licheniformis liquid: bacillus thuringiensis bacterium liquid: the ratio of colloid bacillus cereus bacterium liquid=5-1:2-1:1:1:1, by above-mentioned five kinds of bacterium liquid mixing mixing, obtains mixed bacteria liquid;
The preparation of described chitosan hydrolyzate liquid: get 45-55kg chitosan, adds 865-835kg water, and mixing, is warming up to 95-100 DEG C, then adds phosphatase 79 0-110kg, fully stirs and makes it mixing, obtain chitosan hydrolyzate liquid;
The preparation of described animal blood hydrolyzed solution: get not solidified animal blood, be warming up to 65-70 DEG C, adds animal blood lytic enzyme, hydrolysis 8-12 hour; Be warming up to 100 DEG C after hydrolysis, keep being down to normal temperature after the enzyme that goes out in 1 hour, obtain animal blood hydrolyzed solution;
The consumption of above-mentioned animal blood lytic enzyme is the enzyme of every 1000kg animal blood 3kg.
2. swollen fruit type microbial fertilizer as claimed in claim 1, is characterized in that, described composite trace element, be by etc. the ferrous sulfate of quality, boric acid, manganous sulfate, copper sulfate, zinc sulfate, ammonium molybdate mixes.
3. swollen fruit type microbial fertilizer as claimed in claim 1, is characterized in that, described fermented liquid all at 121 DEG C sterilizing re-use after 2 hours.
4. swollen fruit type microbial fertilizer as claimed in claim 1, is characterized in that, described animal blood is fresh ox blood.
5. swollen fruit type microbial fertilizer as claimed in claim 1, is characterized in that, is warming up to 96 DEG C in the preparation of described chitosan hydrolyzate liquid.
6. swollen fruit type microbial fertilizer as claimed in claim 1, is characterized in that, is warming up to 67 DEG C in the preparation of described animal blood hydrolyzed solution.
7. swollen fruit type microbial fertilizer as claimed in claim 1, it is characterized in that, described swollen fruit type microbial fertilizer, its activeconstituents is formed by following composition proportion by weight: animal blood hydrolyzed solution 65-68 part, chitosan hydrolyzate liquid 73-77 part, composite trace element 8-12 part, potassium primary phosphate 60-68 part, saltpetre 93-100 part, mixed bacteria liquid 680-700 part.
8. swollen fruit type microbial fertilizer as claimed in claim 7, it is characterized in that, described swollen fruit type microbial fertilizer, its activeconstituents is formed by following composition proportion by weight: animal blood hydrolyzed solution 67 parts, chitosan hydrolyzate liquid 75 parts, trace element 10 parts, potassium primary phosphate 64 parts, 96 parts, saltpetre, bacterium liquid 690 parts.
9. the preparation method of the swollen fruit type microbial fertilizer as described in claim as any in claim 1-8, is characterized in that, get each component, mixing mixing.
10. the application of the swollen fruit type microbial fertilizer as described in claim as any in claim 1-8, is characterized in that, the application of described fertilizer in arviculture.
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| CN104478586A (en) * | 2014-12-10 | 2015-04-01 | 山东大学 | Selenium-rich microbial preparation prepared by fermentation of animal serum and preparation method of selenium-rich microbial preparation |
| CN105967961A (en) * | 2016-02-25 | 2016-09-28 | 马鞍山市全润农业科技有限公司 | Rooting-promoting nutritional modified-Chinese-chestnut-protein-coated slow-release fertilizer |
| CN109336673A (en) * | 2018-10-22 | 2019-02-15 | 广西垂青生物科技有限公司 | A kind of mango special liquid compound fertilizer and preparation method thereof |
| CN109503234A (en) * | 2018-12-06 | 2019-03-22 | 中国热带农业科学院农产品加工研究所 | A kind of ammino acid liquid fertilizer and preparation method thereof comprehensively utilizing discarded marine product preparation |
| CN111153744A (en) * | 2020-01-14 | 2020-05-15 | 山东丰本生物科技股份有限公司 | Flower-retention type microbial fertilizer |
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