CN103739375A - Microbial fermentation humic acid biological fertilizer and application and preparation method thereof - Google Patents
Microbial fermentation humic acid biological fertilizer and application and preparation method thereof Download PDFInfo
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- CN103739375A CN103739375A CN201310701079.6A CN201310701079A CN103739375A CN 103739375 A CN103739375 A CN 103739375A CN 201310701079 A CN201310701079 A CN 201310701079A CN 103739375 A CN103739375 A CN 103739375A
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Abstract
The invention relates to an agricultural biological fertilizer and particularly relates to a microbial fermentation humic acid biological fertilizer, an application thereof and a method for preparing the microbial fermentation humic acid biological fertilizer and belongs to the field of fertilizer production; the technical problem to be solved is to provide a humic acid biological fertilizer prepared by a compound microorganism bacterium agent fermentation method; According to the technical solution, the microbial fermentation humic acid biological fertilizer comprises the following components in parts by weight: 100 parts of humic acid fermentation liquor and 15-20 parts of urea; and the microbial fermentation humic acid biological fertilizer provided by the invention can be used as a foliage fertilizer, a water flush fertilizer and a seed soaking agent and has good effects in plant growth and development, disease prevention and treatment, as well as output increase.
Description
Technical field
The present invention relates to a kind of agricultural biological fertile, be specifically related to a kind of microorganism fermentation humic acid biological fertilizer and application thereof, and the method for preparing this microorganism fermentation humic acid biological fertilizer, field of fertilizer production belonged to.
Background technology
The kind of fertilizer is varied, by composition, is generally divided into chemical fertilizer, organic fertilizer and bio-bacterial manure, by purposes, also has foliage fertilizer, punching fertilising etc., segments separately in field, and kind is various especially, countless.Along with agriculture, economic, scientific and technological development, requirement for agricultural fertilizer is more and more higher, conventional fertilizers can not meet the demand of vast farmers, chemical fertilizer, organic fertilizer and bio-bacterial manure etc. are carried out to mutual combination, pursue fertilizer and variation function, become the theme of fertiliser production.
Existing market apply fertilizer is multifarious, very different, and effect is also different.Have simple compositely, have abuse plant-growth regulator, useful agricultural chemicals.Really can realize green, environmental protection, efficient and a fertile multiple-effect, still seldom.
Summary of the invention
The present invention overcomes the deficiencies in the prior art, and it is fertile that technical problem to be solved is to provide humic acid biological prepared by a kind of method of utilizing complex micro organism fungicide fermentation.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of microorganism fermentation humic acid biological is fertile, comprises 100 parts of the humic acid fermentation liquid of parts by weights meter, urea 15-20 part.
Microorganism fermentation humic acid biological fertilizer of the present invention can be used as foliage fertilizer and uses, and also can be used as punching fertilising and uses, and also can soak seed for crop.
When using as foliage fertilizer, in order to increase fertilizer efficiency, can also further add potassium primary phosphate 1-2 part, boric acid 0.1-0.05 part, ferrous sulfate 0.1-0.05 part, copper sulfate 0.1-0.05 part, nitrocalcite 0.2-0.1 part, magnesium sulfate 0.1-0.05 part, zinc sulfate 0.1-0.05 part, manganous sulfate 0.1-0.05 part, amine molybdate 0.01-0.005 part by weight.During for leaf dressing, need to be by fertilizer stoste dilution 800-1000 doubly.
When using as punching fertilising, can also further add phosphoric acid diamines 10-15 part, potassium sulfate 15-20 part by weight.As when fertilising punching, should when irrigated crop, with water, be applied to crop root, 8-10 kilogram of every mu of ground consumption can meet primary demand.
When the present invention is used as seed soaking, need to by fertilizer stoste dilution 800-1000 doubly then soak seed, can improve the percentage of germination of seed, make kind of bud more healthy and strong simultaneously.
The present invention only need be by the material dissolution of other required components in humic acid fermentation liquid.Main feature is that humic acid fermentation liquid used is to obtain by the microorganism humic acids that ferments.
The preparation method of described humic acid fermentation liquid is: first by 100 parts of the raw material humic acid materials of parts by weights meter, 10 parts of analysis for soybean powder, 10 parts of fish meal, 10 parts of glucose, 0.1 part, inorganic salt, 500 parts, water is prepared into substratum according to a conventional method, and wherein inorganic salt are one or more composite in potassium primary phosphate, manganous sulfate, calcium chloride; Then with steam, substratum is carried out to sterilizing, 121 ℃ of sterilizings 20 minutes, displaced air pressurize is afterwards cooled to the suitableeest culture temperature to substratum simultaneously; Complex microbial inoculum is inoculated on substratum by inoculum size 3%, advanced person's aerobe fermentation of acting charitably, fermentation condition is stirring velocity 180r/min, start ventilation ratio and be adjusted to 1:0.5, between incubation period, fill with to press and will remain on 0.05Mpa left and right, along with the continuous growth oxygen dissolving value of thalline also changes gradually, therefore need to adjust ventilation ratio and stirring velocity to make dissolved oxygen relative value 20%-40%, fermentation time 36-48 hour, leavening temperature 32-35 ℃, pH value is 6.5-7.5; Then carry out anaerobically fermenting, fermentation condition is standing for fermentation, fermentation time 48-60 hour, and 32 ℃-35 ℃ of leavening temperatures, stir 1 minute for every six hours; PH value finishes to obtain the humic acid fermentation liquid of degraded for 4.0-4.5 anaerobically fermenting;
Described humic acid material is one or more in ammonium humate, Sodium salts humic acids, potassium humate, weathered coal, brown coal, peat.
Described complex micro organism fungicide is formed by weight 1.8:1.8:1.8:1:1:1:1:1 is composite by subtilis, Bacillus licheniformis, bacillus pumilus, yeast saccharomyces cerevisiae, plant lactobacillus, Lactobacterium acidophilum, streptococcus uberis and bacillus aceticus.
Compared with prior art the present invention has following beneficial effect.
Bacillus category, in growth fermenting process, is secreted many organized enzymes, as amylase, proteolytic enzyme, cellulase, hemicellulase etc.Secrete many bacitracins, exocellular polysaccharide, these materials to pathogenic bacteria, have the effect that suppresses and kill and wound, also secrete some somatomedins.So the output of growing, prevent and cure diseases, improve to plant has good effect.
The humic acid fermentation liquid that the present invention adopts, has overcome the difficult problem of current humic acids difficult degradation, to humic acids, adopts microbial degradation method further to degrade, and makes it be converted into small molecules solubility xanthohumic acid.Directly degrading weathered coal, peat etc. are containing the humic acids in the higher low-rank coal of humic acids, than direct employing low-rank coal, sneak in fertilizer or make compost many that effect will be good.
The present invention has improved the utilization ratio of resource; Expanded humic acids range of application.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.
Complex micro organism fungicide described in each embodiment is formed by weight 1.8:1.8:1.8:1:1:1:1:1 is composite by subtilis, Bacillus licheniformis, bacillus pumilus, yeast saccharomyces cerevisiae, plant lactobacillus, Lactobacterium acidophilum, streptococcus uberis and bacillus aceticus.
Embodiment 1
A kind of microorganism fermentation humic acid biological is fertile, comprises 100 parts of the humic acid fermentation liquid of parts by weights meter, 15 parts, urea.
The preparation method of humic acid fermentation liquid: first by 100 parts of the raw material humic acid materials of parts by weights meter, 10 parts of analysis for soybean powder, 10 parts of fish meal, 10 parts of glucose, 0.1 part, inorganic salt, 500 parts, water is prepared into substratum according to a conventional method, and wherein inorganic salt are one or more composite in potassium primary phosphate, manganous sulfate, calcium chloride; Then with steam, substratum is carried out to sterilizing, 121 ℃ of sterilizings 20 minutes, displaced air pressurize is afterwards cooled to the suitableeest culture temperature to substratum simultaneously; Complex microbial inoculum is inoculated on substratum by inoculum size 3%, advanced person's aerobe fermentation of acting charitably, fermentation condition is stirring velocity 180r/min, start ventilation ratio and be adjusted to 1:0.5, between incubation period, fill with to press and will remain on 0.05Mpa left and right, along with the continuous growth oxygen dissolving value of thalline also changes gradually, therefore need to adjust ventilation ratio and stirring velocity that dissolved oxygen relative value is progressively increased in 20%-40% scope, leavening temperature be controlled at 32-35 ℃ fermentation 48 hours, to pH value be 7.2; Then carry out anaerobically fermenting, fermentation condition is standing for fermentation, and leavening temperature is controlled at 32 ℃-35 ℃ fermentations 48 hours, within every six hours, stirs 1 minute; Detecting pH value is the humic acid fermentation liquid that 4.5 anaerobically fermentings finish to obtain degraded.
Embodiment 2
A kind of microorganism fermentation humic acid biological is fertile, comprises 100 parts of the humic acid fermentation liquid of parts by weights meter, 20 parts, urea.
The preparation method of humic acid fermentation liquid: first by 100 parts of the raw material humic acid materials of parts by weights meter, 10 parts of analysis for soybean powder, 10 parts of fish meal, 10 parts of glucose, 0.1 part, inorganic salt, 500 parts, water is prepared into substratum according to a conventional method, and wherein inorganic salt are one or more composite in potassium primary phosphate, manganous sulfate, calcium chloride; Then with steam, substratum is carried out to sterilizing, 121 ℃ of sterilizings 20 minutes, displaced air pressurize is afterwards cooled to the suitableeest culture temperature to substratum simultaneously; Complex microbial inoculum is inoculated on substratum by inoculum size 3%, advanced person's aerobe fermentation of acting charitably, fermentation condition is stirring velocity 180r/min, start ventilation ratio and be adjusted to 1:0.5, between incubation period, fill with to press and will remain on 0.05Mpa left and right, along with the continuous growth oxygen dissolving value of thalline also changes gradually, therefore need to adjust ventilation ratio and stirring velocity that dissolved oxygen relative value is progressively increased in 20%-40% scope, leavening temperature be controlled at 32-35 ℃ fermentation 43 hours, to pH value be 7.5; Then carry out anaerobically fermenting, fermentation condition is standing for fermentation, and leavening temperature is controlled at 32 ℃-35 ℃ fermentations 60 hours, within every six hours, stirs 1 minute; Detecting pH value is the humic acid fermentation liquid that 4.1 anaerobically fermentings finish to obtain degraded.
Embodiment 3
A kind of microorganism fermentation humic acid biological is fertile, comprises 100 parts of the humic acid fermentation liquid of parts by weights meter, 18 parts, urea.
The preparation method of humic acid fermentation liquid: first by 100 parts of the raw material humic acid materials of parts by weights meter, 10 parts of analysis for soybean powder, 10 parts of fish meal, 10 parts of glucose, 0.1 part, inorganic salt, 500 parts, water is prepared into substratum according to a conventional method, and wherein inorganic salt are one or more composite in potassium primary phosphate, manganous sulfate, calcium chloride; Then with steam, substratum is carried out to sterilizing, 121 ℃ of sterilizings 20 minutes, displaced air pressurize is afterwards cooled to the suitableeest culture temperature to substratum simultaneously; Complex microbial inoculum is inoculated on substratum by inoculum size 3%, advanced person's aerobe fermentation of acting charitably, fermentation condition is stirring velocity 180r/min, start ventilation ratio and be adjusted to 1:0.5, between incubation period, fill with to press and will remain on 0.05Mpa left and right, along with the continuous growth oxygen dissolving value of thalline also changes gradually, therefore need to adjust ventilation ratio and stirring velocity that dissolved oxygen relative value is progressively increased in 20%-40% scope, leavening temperature be controlled at 32-35 ℃ fermentation 36 hours, to pH value be 6.7; Then carry out anaerobically fermenting, fermentation condition is standing for fermentation, and leavening temperature is controlled at 32 ℃-35 ℃ fermentations 54 hours, within every six hours, stirs 1 minute; Detecting pH value is the humic acid fermentation liquid that 4.3 anaerobically fermentings finish to obtain degraded.
Embodiment 4
A kind of microorganism fermentation humic acid biological foliage fertilizer, on the basis of embodiment 1, also comprise 0.05 part of 0.1 part, 0.1 part, 0.1 part, 0.1 part, 0.1 part, 0.1 part of 1 part of potassium primary phosphate, boric acid, ferrous sulfate, copper sulfate, nitrocalcite, magnesium sulfate, zinc sulfate, manganous sulfate, 0.005 part of amine molybdate by weight.
Embodiment 5
A kind of microorganism fermentation humic acid biological foliage fertilizer, on the basis of embodiment 2, also comprise 0.1 part of 0.05 part, 0.05 part, 0.2 part, 0.1 part, 0.05 part, 0.05 part of 2 parts of potassium primary phosphates, boric acid, ferrous sulfate, copper sulfate, nitrocalcite, magnesium sulfate, zinc sulfate, manganous sulfate, 0.01 part of amine molybdate by weight.
Embodiment 6
A kind of microorganism fermentation humic acid biological punching fertilising, on the basis of embodiment 1, also comprises 10 parts of phosphoric acid diamines, 20 parts of potassium sulfate by weight.
Embodiment 7
A kind of microorganism fermentation humic acid biological punching fertilising, on the basis of embodiment 1, also comprises 15 parts of phosphoric acid diamines, 15 parts of potassium sulfate by weight.
Experimental example
One, cotton seed soaking test
For trying crop varieties: early No. 8, the new land of cotton.
Set handling: 10 times of diluents, 100 diluents, 1000 times of diluents, contrast clear water.
Hank 50 of ripe, full, normal cotton seeds of color and luster, in 27 ℃ of incubators, four kinds of soaked with liquid 16 hours.
Experimental result shows that 1000 times of diluents of foliage fertilizer improve 12% than Seed soaking percentage of germination, and plumule shows money or valuables one carries unintentionally thick and strengthens.
Two, this tests made foliage fertilizer, the result for the treatment of of punching fertilising to jujube tree, Chlorotic disorder of peach tree, little leaf disease.
Experiment place: Zeng Jiaying village, Qing Tou town, Yongji City, Shanxi Province
For studying thing: jujube tree, peach
Fruit diseases symptom: occur the phenomenon that leaf flavescence, leaflet are grown thickly in the jujube tree of cultivation, peach woods, conventional method is that root is filled with copperas solution.This experiment is to spray plant leaf surface with 1000 times of diluents, fills with root 10kg liquid simultaneously.After three days, it is green that leaf turns, and recovers normal after one week, and two weeks rear blades thicken, black green.Select altogether for examination jujube tree 35 strains, peach 52 strains, efficient 100%, curative ratio 85%.Cause the reason of yellowtop, little leaf disease to be mostly what nutrition or malabsorption caused, edatope and trophic factor are major causes, and also having is individually that virus causes, the method applied in the present invention is the factor that comprehensively has additional nutrients, improve edatope, increase plant receptivity.And the disease causing for virus also has certain result for the treatment of.
Three, plastic tent cucumber volume increase experiment
For trying place: Nan Jia village, Yuncheng City of Shanxi
For studying thing: plastic tent cucumber
Test method: 1000 times of diluents of foliage fertilizer spray blade face, and 15 days once.Punching fertilising is before, during and after the production process respectively fills with root 1 time.0.9 mu of booth area, 0.8 mu of experiment, 0.1 mu of contrast normal management.
Experimental result shows: every 66.7 square metres of volume increase 358kg, stimulation ratio 57%.
Four, grape experiment
Place: the Yong Xi village, Qing Tou town, Yongji City, Shanxi Province
Experiment kind: black treasured early
Experimental period: 2009 to 2011
Test method: with microbiological deterioration humic acid solution, as organic fertilizer fermentation agent, organic materials is fermented, the fertilizer that obtains becoming thoroughly decomposed.Concrete batching: chicken manure 50%, straw part 20%, weathered coal 20%, 10% ground phosphate rock, humidity 55%, 3% starter.Heap fermentation 10-15 days, 65 ℃ of maintenances of leavening temperature three days, kill pathogens and worm's ovum.By every mu of 1000kg, apply wine-growing ditch, every mu of 330 strains of planting density.Field planting in autumn in 2009,2011 annual bearings
Experiment shows that field planting Second Year just reaches the normal management level output of the 3rd year with this product plantation grape.
In above-mentioned experimental example, employing be the bio-fertilizer that only contains humic acid fermentation liquid and urea.
The present invention can summarize with other the specific form without prejudice to spirit of the present invention or principal character.Therefore, no matter from that, above-mentioned embodiment of the present invention all can only think explanation of the present invention can not limit invention, claims have been pointed out scope of the present invention, and scope of the present invention is not pointed out in above-mentioned explanation, therefore,, in the implication suitable with claims of the present invention and any variation in scope, all should think to be included in the scope of claims.
Claims (9)
1. microorganism fermentation humic acid biological is fertile, it is characterized in that comprising 100 parts of the humic acid fermentation liquid of parts by weights meter, urea 15-20 part.
2. the preparation method of a kind of microorganism fermentation humic acid biological fertilizer according to claim 1, it makes for described urea is dissolved in described humic acid fermentation liquid, it is characterized in that: the preparation method of described humic acid fermentation liquid, first by 100 parts of the raw material humic acid materials of parts by weights meter, 10 parts of analysis for soybean powder, 10 parts of fish meal, 10 parts of glucose, 0.1 part, inorganic salt, 500 parts, water is prepared into substratum according to a conventional method, and wherein inorganic salt are one or more composite in potassium primary phosphate, manganous sulfate, calcium chloride; Then with steam, substratum is carried out to sterilizing, 121 ℃ of sterilizings 20 minutes, displaced air pressurize is afterwards cooled to the suitableeest culture temperature to substratum simultaneously; Complex microbial inoculum is inoculated on substratum by inoculum size 3%, advanced person's aerobe fermentation of acting charitably, fermentation condition is stirring velocity 180r/min, start ventilation ratio and be adjusted to 1:0.5, between incubation period, fill with to press and will remain on 0.05Mpa left and right, along with the continuous growth oxygen dissolving value of thalline also changes gradually, therefore need to adjust ventilation ratio and stirring velocity to make dissolved oxygen relative value 20%-40%, fermentation time 36-48 hour, leavening temperature 32-35 ℃, pH value is 6.5-7.5; Then carry out anaerobically fermenting, fermentation condition is standing for fermentation, fermentation time 48-60 hour, and 32 ℃-35 ℃ of leavening temperatures, stir 1 minute for every six hours; PH value finishes to obtain the humic acid fermentation liquid of degraded for 4.0-4.5 anaerobically fermenting;
Described humic acid material is one or more in ammonium humate, Sodium salts humic acids, potassium humate, weathered coal, brown coal, peat.
3. the preparation method of a kind of microorganism fermentation humic acid biological fertilizer according to claim 2, is characterized in that: described complex micro organism fungicide is formed by weight 1.8:1.8:1.8:1:1:1:1:1 is composite by subtilis, Bacillus licheniformis, bacillus pumilus, yeast saccharomyces cerevisiae, plant lactobacillus, Lactobacterium acidophilum, streptococcus uberis and bacillus aceticus.
4. a kind of microorganism fermentation humic acid biological according to claim 1 is fertile, it is characterized in that: also comprise potassium primary phosphate 1-2 part, boric acid 0.1-0.05 part, ferrous sulfate 0.1-0.05 part, copper sulfate 0.1-0.05 part, nitrocalcite 0.2-0.1 part, magnesium sulfate 0.1-0.05 part, zinc sulfate 0.1-0.05 part, manganous sulfate 0.1-0.05 part, amine molybdate 0.01-0.005 part by weight.
5. fertile according to a kind of microorganism fermentation humic acid biological described in claim 1 or 4, it is characterized in that: it is applied to crop foliage fertilizer.
6. a kind of microorganism fermentation humic acid biological according to claim 1 is fertile, it is characterized in that: also comprise phosphoric acid diamines 10-15 part, potassium sulfate 15-20 part by weight.
7. fertile according to a kind of microorganism fermentation humic acid biological described in claim 1 or 6, it is characterized in that: it is applied to crop punching fertilising.
8. fertile according to a kind of microorganism fermentation humic acid biological described in claim 1 or 4 or 6, it is characterized in that: it is applied to crop seed soaking.
9. a kind of microorganism fermentation humic acid biological according to claim 8 is fertile, it is characterized in that: described microorganism is fermented to the fertile dilution of humic acid biological 800-1000 doubly, diluent is soaked seed for crop.
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| CN106107023A (en) * | 2016-08-26 | 2016-11-16 | 北京斯隆生物技术有限责任公司 | A kind of microbial degradation humic acids feedstuff and preparation method thereof |
| CN106348848A (en) * | 2016-08-23 | 2017-01-25 | 山东蓝驼农业技术开发有限公司 | Method for compounding biological fertilizer capable of improving fruit taste |
| CN106905050A (en) * | 2017-03-25 | 2017-06-30 | 山东福龙生物化肥有限公司 | A kind of feature fruit tree special bio microbial inoculum |
| CN107473850A (en) * | 2017-09-19 | 2017-12-15 | 山西浩之大生物科技有限公司 | A kind of method for improving humic acid matrix-based middle fulvo acid content |
| CN107573193A (en) * | 2017-09-27 | 2018-01-12 | 安徽金叶碳素科技有限公司 | A kind of microbial degradation lignite compounding liquid fertilizer and preparation method thereof |
| WO2019075536A1 (en) * | 2017-10-18 | 2019-04-25 | Hokari Diro | Method for producing a bioactivated, liquid, organic compound and product obtained |
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| CN111621063A (en) * | 2020-06-09 | 2020-09-04 | 河南功能高分子膜材料创新中心有限公司 | Easily degradable humic acid mulching film |
| CN112841225A (en) * | 2021-02-20 | 2021-05-28 | 东北农业大学 | Microbial preparation for preventing and controlling rotting of cellaring potatoes and application of microbial preparation |
| CN115504830A (en) * | 2022-09-21 | 2022-12-23 | 河北萌帮生物科技有限公司 | Microbial fermentation biological humic acid, preparation method and application |
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| CN111621063A (en) * | 2020-06-09 | 2020-09-04 | 河南功能高分子膜材料创新中心有限公司 | Easily degradable humic acid mulching film |
| CN111574308A (en) * | 2020-06-12 | 2020-08-25 | 中国热带农业科学院椰子研究所 | Special liquid fertilizer for young areca nuts and application method thereof |
| CN112841225A (en) * | 2021-02-20 | 2021-05-28 | 东北农业大学 | Microbial preparation for preventing and controlling rotting of cellaring potatoes and application of microbial preparation |
| CN115504830A (en) * | 2022-09-21 | 2022-12-23 | 河北萌帮生物科技有限公司 | Microbial fermentation biological humic acid, preparation method and application |
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Application publication date: 20140423 |