CN107892609A - One kind utilizes fermentation method production amino acid bio-organic fertilizer and preparation method thereof - Google Patents
One kind utilizes fermentation method production amino acid bio-organic fertilizer and preparation method thereof Download PDFInfo
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- CN107892609A CN107892609A CN201710356028.2A CN201710356028A CN107892609A CN 107892609 A CN107892609 A CN 107892609A CN 201710356028 A CN201710356028 A CN 201710356028A CN 107892609 A CN107892609 A CN 107892609A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The present invention relates to biological fermentation engineering field.Specifically provide one kind by agriculture, herd, caused organic waste in fish production, be amino acid bio-organic fertilizer and preparation method thereof using bio-fermentation process Rapid Fermentation.It will tame respectively to the mixed bacteria liquid being made up of saccharomycete and bacillus that can reach under the conditions of Co metabolism, it is seeded in the fermentation medium being made up of carbon source, nitrogen source, inorganic salts and organic matter through high-temperature sterilization, using the general fermentation tank of open type, carry out fermented and cultured 10 15 days, separation supernatant produces amino acid bio-organic fertilizer.Fertilizer of the present invention be by agriculture, herd, the organic waste fast decoupled of fishery is converted into amino acid, multivitamin, the biology enzyme that plant available utilizes; Antibiotics material and the organic bio-fertilizer containing biological bacteria; it is to increase soil fertility; improve crop quality; yield is improved, beneficial to the green high quality fertilizer of environmental protection.
Description
Technical field
The present invention relates to biological fermentation engineering field.Specifically provide it is a kind of by agriculture, herd, in fish production it is caused organic
Discarded object, it is amino acid bio-organic fertilizer and preparation method thereof using bio-fermentation process Rapid Fermentation.
Background technology
Amino acid bio-organic fertilizer is indispensable high-quality in agricultural production, free of contamination fertilizer.To improving
The yield of crops, improve the quality of crops and recover due to it is long-term largely using chemical fertilizer and caused by soil fertility decline and have
Obvious action.Agriculture simultaneously, is herded, caused organic waste is also faced with the quick processing of needs in fish production, is turned waste into wealth
The problems such as.In the market more than the amino acid Water soluble fertilizer using fish as main material production based on chemical reaction flows.Its shortcoming is
Active ingredient is single, inactive material.There is also the pollution of acid-base pair environment in production.The present invention is making full use of agriculture,
Herd, discarded object is produced in fishery production, quickly produced using biological fermentation process containing amino acid, polypeptide, multivitamin, biology
Enzyme, anti-microbial type material, the organic fertilizer of biological bacteria.Easily absorb, increase production with plant, quality is improved, the features such as fertilizing soil.Production
Process is pollution-free.Short with fermentation time compared with traditional organic fertilizer fermentation reactor system, floor space is small, and product miscellaneous bacteria is few, effectively
The advantages that component content is high.
The content of the invention
Present invention aims at providing one kind to utilize biological fermentation process, by agriculture, herd, caused organic waste in fish production
The method that thing fast decoupled is converted into amino acid bio-organic fertilizer.
To achieve the above object, the technical solution adopted by the present invention is：
One kind will be tamed under the conditions of can reach Co metabolism respectively using fermentation method production amino acid bio-organic fertilizer
The mixed bacteria liquid being made up of saccharomycete and bacillus, it is seeded in through high-temperature sterilization by carbon source, nitrogen source, inorganic salts and organic matter
In the fermentation medium of composition, using the general fermentation tank of open type, fermented and cultured is carried out 10-15 days, separation supernatant produces ammonia
Base acid biological organic fertilizer, wherein, saccharomycete is Candida lipolytica, pichia farinose and S. cervisiae, and bacillus is
Bacillus subtillis and wax bacillus.
Above-mentioned acquisition organic fertilizer be containing several amino acids, vitamin, biology enzyme, anti-microbial type material, nitrogen, phosphorus, potassium, calcium,
The amino acid bio-organic fertilizer of magnesium and biologic live bacteria.Contain 60-80% organic matters, amino acid, nitrogen, phosphorus, potassium and mineral in residue
Prime element and biologic live bacteria.
The Candida lipolytica, pichia farinose, saccharomyces cerevisiae, Bacillus subtillis, wax bacillus difference
In high salt, high sugar, and enriched containing organic protein, PH is culture in neutral culture medium to normal growth and then realizes domestication,
Then by the strain after above-mentioned domestication respectively, then pure culture is carried out respectively, bacterium solution is according to yeast：Bacillus is with 1:1.5-2 body
Product ratio mixing.
It is above-mentioned to be domesticated in ammonium sulfate 2-7%；Diammonium hydrogen phosphate 2-7%, magnesium sulfate 0.5-3%, potassium sulfate 1-3%, Portugal
Cultivated in grape sugar 5-10%, and the bean powder 5-10% enriched containing organic protein culture, culture PH is neutrality, then by dense
Degree increases principle successively, and progressively culture is in sugar, up to 10%, inorganic salts total amount is up to can normal growth in 12% culture
Untill.
Above-mentioned bacillus has the ability of extremely strong big aminosal under adequate nutrients supply conditions, decomposes production
A variety of thing is amino acid, and which part amino acid can then can absorb part as the nutrition needed for growth and development of plants, saccharomycete
Amino acid, decompose using the fat in culture medium and secret out of the metabolites such as biology enzyme, vitamin, these metabolites have
Growth and development of plants is stimulated, increases the effect of vital metabolic activity in plant.
Carbon source is glucose in the fermentation medium, sucrose, starch, and one kind in molasses, content is fermentation medium
The 5-10% of weight；Nitrogen source is fermentation medium weight 3-5% urea, fermentation medium weight 1-3% ammonium sulfate, is fermented
Culture medium weight 2-3% diammonium hydrogen phosphate；Inorganic salts are fermentation medium weight 1-2% potassium sulfate, fermentation medium weight
Measure one kind in 3-5% calcium carbonate, calcium superphosphate, fermentation medium weight 0.5-1% magnesium sulfate；Organic matter be trash fish,
Meat mincing, grouts, two or three of content of wheat bran are the 25-40% of fermentation medium weight.
The fermentate stirs in the general fermentation tank of open type at 28-32 DEG C of PH4-6, temperature, 60-100rpm revolutions
Or fermented and cultured under intermittent stirring.
The organic fertilizer is applied fertilizer as punching or fertilizer for spraying on leaf surface, available for field crop, in vegetables or fruit tree.
A kind of preparation method that biological organic fertilizer is produced using fermentation method, by saccharomycete and brood cell's bar after taming respectively
Bacterium, the mixed bacteria liquid formed after pure culture, it is seeded in and is made up of through high-temperature sterilization carbon source, nitrogen source, inorganic salts and organic matter
Fermentation medium in, using the general fermentation tank of open type, carry out fermented and cultured 10-15 days, separation supernatant produces amino acid
Biological organic fertilizer, wherein, saccharomycete is Candida lipolytica, pichia farinose and S. cervisiae, and bacillus is withered grass
Bacillus and wax bacillus.
Carbon source is glucose in the fermentation medium, sucrose, starch, and one kind in molasses, content is fermentation medium
The 5-10% of weight；Nitrogen source is fermentation medium weight 3-5% urea, fermentation medium weight 1-3% ammonium sulfate, is fermented
Culture medium weight 2-3% diammonium hydrogen phosphate；Inorganic salts are fermentation medium weight 1-2% potassium sulfate, fermentation medium weight
One kind, fermentation medium weight 0.5-1% magnesium sulfate in amount 3-5% calcium carbonate or calcium superphosphate；Organic matter be trash fish,
Meat mincing, grouts, two or three of content of wheat bran are the 25-40% of fermentation medium weight.
The Candida lipolytica, pichia farinose, saccharomyces cerevisiae, Bacillus subtillis, wax bacillus difference
In high salt, high sugar, and enriched containing organic protein, PH is culture in neutral culture medium to normal growth and then realizes domestication,
Then by the strain after above-mentioned domestication respectively, then pure culture is carried out respectively, bacterium solution is according to yeast：Bacillus is with 1:1.5-2 body
Product ratio mixing.
Candida lipolytica in the yeast, pichia farinose, saccharomyces cerevisiae press 1:1:1 volume ratio mixes；
Bacillus subtillis in bacillus, wax bacillus press 1:1 volume ratio mixes.
Advantage for present invention：
The fermented acquisition organic matter of the present invention, which is rich in, amino acid, polypeptide, multivitamin, biology enzyme, anti-microbial type material.
And contain the elements such as a number of nitrogen, phosphorus, potassium, calcium, magnesium.The bio-organic fertilizer containing amino acid produced using this method
Compared with the amino acids fertilizer that chemical method produces, complete with amino acid classes, bioactive substance species is more, and plant is easy
Absorb, the advantages that production process is pollution-free；It is short with fermentation time compared with traditional organic fertilizer fermentation reactor system, floor space
Small, product quality is high, the advantages that no miscellaneous bacteria.
Embodiment
The application is further explained with reference to embodiment.
Fertilizer of the present invention be by agriculture, herd, the organic waste fast decoupled of fishery is converted into the ammonia that plant available utilizes
Base acid, multivitamin, biology enzyme, Antibiotics material and the organic bio-fertilizer containing biological bacteria, are to increase soil fertility, and are improved
Crop quality, yield is improved, beneficial to the green high quality fertilizer of environmental protection.It is primarily characterized in that：Strain combination optimizes, product
The features such as component is diversified, low production cost, and using effect is obvious.Component is as follows：Amino acid 8-10%；Nitrogen 4-6%；Phosphorus
1%-1.2%；Potassium 0.8%-1%；Calcium, sulphur, magnesium amounts to 0.5%-3%, biological bacteria >=0.2 hundred million/milliliter, and contains vitamin B1,
A variety of B family vitamins such as B2, B6 nicotinic acid, a variety of biology enzymes and anti-microbial type material.There are the present invention raw material to be easy to get extensively, produce
Equipment is simple, and cost is cheap, with short production cycle, the advantages that being adapted to mass produce.
Embodiment 1：
One ton tank fermenting and producing method:
1. tamed strain：By saccharomycete, each bacterial strain of bacillus is in ammonium sulfate 2-7%；Diammonium hydrogen phosphate 2-7%, sulphur
Trained in sour magnesium 0.5-3%, potassium sulfate 1-3%, glucose 5-10%, and the abundant bean powder 5-10% containing organic protein culture
Support, culture PH is neutrality, then increases principle successively by concentration, and progressively culture is in sugar, up to 10%, inorganic salts total amount reaches
Can be untill normal growth in 12% culture.Bacillus subtilis after domestication, Bacillus cereus are respectively in beef
On cream-peptone slant medium, 28-30 DEG C is cultivated 20 hours；Candida lipolytica, S. cervisiae, pichia farinose
Cultivated 30 hours for 28-30 DEG C on glucose-yeast extract slant medium.
2. by above-mentioned saccharomycete, bacillus respectively in 10% bean powder that content is fermentation medium weight, 5% grape
Sugar, in the fermentation medium of 2% ammonium sulfate, 30-35 DEG C of concussion and cultivate 30 hours.The liter of saccharomycete 20, the liter of bacillus 30
Mix stand-by.
Bacterium solution is according to yeast：Bacillus is with 1:2 volume ratios mix.
Candida lipolytica in the yeast, pichia farinose, saccharomyces cerevisiae press 1:1:1 volume ratio mixes；
Bacillus subtillis in bacillus, wax bacillus press 1:1 volume ratio mixes.
3. fermenter volume is one ton, the culture medium total amount that feeds intake is about 700 kilograms.Wherein 200 kilograms of iced trash fish, grouts 20
Kilogram, 40 kilograms of sucrose, 5 kilograms of ammonium sulfate, 25 kilograms of urea, 8 kilograms of potassium sulfate, 4 kilograms of magnesium sulfate, 10 kilograms of calcium carbonate,
450-500 kilograms of water.Steam sterilizing 30 minutes, will physically well develop when temperature is down to 35-40 DEG C, the stand-by seed liquor 50 of mixture
Liter is gone in fermentation tank, is stirred with 100 revs/min of speed, intermittent stirring is taken after three days, daily with 100 revs/min
Speed is stirred twice, one hour every time.PH 4-6, ferment 10-12 days.
4. by filtering fermentation liquor, supernatant is centrifuged to obtain, through the detection of Shenyang City's metrological supervision detection research institute：Total amino acid content
For 10.3%, nitrogen 4.3%, phosphorus pentoxide 1.95%, potassium oxide 0.92%, residue organic matter containing 40-60% is obtained after centrifugation, can
It is used as base fertilizer..
Embodiment 2：
The production method of five ton tanks fermentation：
1. tamed strain：By saccharomycete, each bacterial strain of bacillus is in ammonium sulfate 2-7%；Diammonium hydrogen phosphate 2-7%, sulphur
Trained in sour magnesium 0.5-3%, potassium sulfate 1-3%, glucose 5-10%, and the abundant bean powder 5-10% containing organic protein culture
Support, culture PH is neutrality, then increases principle successively by concentration, and progressively culture is in sugar, up to 10%, inorganic salts total amount reaches
Can be untill normal growth in 12% culture.Bacillus subtilis after domestication, Bacillus cereus are respectively in beef
On cream-peptone slant medium, 28-30 DEG C is cultivated 20 hours；Candida lipolytica, S. cervisiae, pichia farinose
28-30 DEG C of culture, Kolle flask is extended to by inclined-plane on glucose-yeast extract slant medium.
2. Spawn incubation：Fermentation tank is 500 liter general-purpose type fermentation tanks, and two class bacterium respectively cultivate 350 kilograms of bacterium solutions.Culture medium
For：Bean powder 15%, glucose 7%, ammonium sulfate 2%, 350 kilograms of water.Sterilising conditions：1Mpa, 30 minutes.Treat that temperature is down to 35 °
When, it is respectively connected to the saccharomycete after activating, bacillus.Fermentation condition：Tank presses 0.05Mpa, air quantity 1：0.5V/V, mixing speed
180rpm, 28-30 DEG C, fermentation time 24 hours.
Bacterium solution is according to yeast：Bacillus is with 1:1.5 volume ratios mix.
Candida lipolytica in the yeast, pichia farinose, saccharomyces cerevisiae press 1:1:1 volume ratio mixes；
Bacillus subtillis in bacillus, wax bacillus press 1:1 volume ratio mixes.
3. fermentation tank is 5 tons.The total amount that feeds intake is 3.5 tons of wherein 350 kilograms of air-drying fishs, 150 kilograms of grouts, and urea 150 is public
Jin, 15 kilograms of ammonium sulfate, 40 kilograms of dipotassium hydrogen phosphate, 20 kilograms of magnesium sulfate, 150 kilograms of calcium superphosphate, 3 tons of water, logical steam goes out
Bacterium 30 minutes, when temperature is down to 35-40 DEG C, the strain in two seeding tanks is depressed into fermentation tank respectively, mixing speed
60rpm, PH 4-6,30-35 DEG C.Later stage can intermittent stirring, mixing speed 60rpm, twice daily, 1 hour every time.Fermented and cultured
15 days.
4. by filtering fermentation liquor, supernatant is centrifuged to obtain.Through the detection of Shenyang City's metrological supervision detection research institute：Total amino acid content is
11.8%th, nitrogen 4.5%, phosphorus pentoxide 2.97%, potassium oxide 1.72%, residue organic matter containing 50-70% is obtained after centrifugation, can be used
Make base fertilizer.
National NY1429- has been reached by the visible present invention gained amino acid bio-organic fertilizer leading indicator of the above results
The execution standard of 2010. defineds.
1. it is high salt by acclimation conditions, high sugar, PH is neutrality, and is cultivated in the culture medium for the training enriched containing organic protein
Middle normal growth, decomposition organic protein is several amino acids, and secretes the bacillus subtilis of the metabolites such as a variety of biology enzymes
Bacterium, Bacillus cereus respectively on beef extract-peptone slant medium 28-30 DEG C cultivate 20 hours；Will be through above-mentioned same
The Candida lipolytica yeast of method domestication, S. cervisiae, pichia farinose is respectively in glucose-yeast extract inclined-plane culture
Cultivated 30 hours for 28-30 DEG C on base, inclined-plane is extended to Kolle flask.
2. Spawn incubation：Fermentation tank is 500 liter general-purpose type fermentation tanks, and two class bacterium respectively cultivate 350 kilograms of bacterium solutions.Culture medium
For：Bean powder 15%, glucose 7%, ammonium sulfate 2%, 350 kilograms of water.Sterilising conditions：1Mpa, 30 minutes.Treat that temperature is down to 35 °
When, it is respectively connected to the saccharomycete after activating, bacillus.Fermentation condition：Tank presses 0.05Mpa, air quantity 1：0.5V/V, mixing speed
180rpm, 28-30 DEG C, fermentation time 24 hours.
3. fermentation tank is 5 tons.The total amount that feeds intake is 3.5 tons of wherein 350 kilograms of air-drying fishs, 150 kilograms of grouts, and urea 25 is public
Jin, 25 kilograms of ammonium sulfate, 40 kilograms of dikalium phosphate, 20 kilograms of magnesium sulfate, 150 kilograms of calcium superphosphate, 3 tons of water, lead to steam sterilizing
30 minutes, when temperature is down to 35-40 DEG C, the strain in two seeding tanks is depressed into fermentation tank respectively, mixing speed 60rpm,
PH 4-6,30-35 DEG C.Later stage can intermittent stirring, mixing speed 60rpm, twice daily, 1 hour every time.Fermented and cultured 15 days.
4. by filtering fermentation liquor, supernatant is centrifuged to obtain.Through the detection of Shenyang City's metrological supervision detection research institute：Total amino acid content is
11.8%th, nitrogen 4.5%, phosphorus pentoxide 2.97%, potassium oxide 1.72%, residue organic matter containing 50-70% is obtained after centrifugation, can be used
Make base fertilizer.
National NY1429- has been reached by the visible present invention gained amino acid bio-organic fertilizer leading indicator of the above results
The execution standard of 2010. defineds.
Application examples 1：
2016, Liaozhong area of Liaoning Province Pan Jia forts peasant was used for tomato planting.Control group applies fertilizer to the subsoil for every mu with experimental group
For 4000 kilograms of farm manures, nitrogen-phosphorus-potassium content is 80 kilograms of 17-17-17 composite fertilizers.Post flowering, control group is at interval of 12-15
My god, punching applies every mu of fulvic acid 7.5 kilograms every time, continuous 5 times.The amino acid bio that above-described embodiment acquisition is applied in experimental group punching has
Machine fertilizer.Every mu 8 kilograms every time, continuous 5 times.It is more dark green than control group leaf color to show as experimental group, plant height increases 2.3 centimetres, individual plant
Fruit increase by 0.64, single fruit increases weight 3.4 grams.Just, fruit color is consistent, in good taste for fruit shape.Mu rate of growth is 7.57-10.32%.
Application examples 2
Shenyang Gao Huaxiang peasant in 2015 transplants for land strawberry.Base fertilizer is the organic fertilizer of the said goods residue mixture.
400 kilograms every mu.Control group base fertilizer is 25 kilograms of every mu of the composite fertilizer that nitrogen-phosphorus-potassium is 20-28-10.Post flowering, every 7-10
It every mu punching applies 8 kilograms of above-described embodiments and obtains amino acid bio-organic fertilizers, continuous 5 times.It is 18- that nitrogen-phosphorus-potassium is applied in control group punching
3.5 kilograms of 18-18 humic acid compound flushing fertilizer, every 7-10 days, continuous 5 times.Experimental group is shown as than control group raising to bear fruit
Rate 10-20%, fruit shift to an earlier date maturation one week, and pol can improve 1-2 degree.Extend storage life 5-7 days.Mu volume increase 10.3-13.2%.
Claims (9)
1. one kind utilizes fermentation method production amino acid bio-organic fertilizer, it is characterised in that：It will tame respectively to common generation can be reached
The mixed bacteria liquid being made up of saccharomycete and bacillus under the conditions of thanking, it is seeded in through high-temperature sterilization by carbon source, nitrogen source, inorganic
In the fermentation medium of salt and organic matter composition, using the general fermentation tank of open type, fermented and cultured is carried out 10-15 days, in separation
Clear liquid produces amino acid bio-organic fertilizer, wherein, saccharomycete is Candida lipolytica, pichia farinose and S. cervisiae,
Bacillus is Bacillus subtillis and wax bacillus.
2. utilize fermentation method production amino acid bio-organic fertilizer as described in claim 1, it is characterised in that：The solution fat vacation silk
Yeast, pichia farinose, saccharomyces cerevisiae, Bacillus subtillis, wax bacillus is respectively in high salt, high sugar, and contain organic
What albumen enriched, PH tames for culture in neutral culture medium to normal growth and then realization, then by after above-mentioned domestication respectively
Strain, then carry out pure culture respectively, bacterium solution is according to yeast：Bacillus is with 1:1.5-2 volume ratios mix.
3. utilize fermentation method production amino acid bio-organic fertilizer as described in claim 1, it is characterised in that：The fermented and cultured
Carbon source is glucose in base, sucrose, starch, one kind in molasses, and content is the 5-10% of fermentation medium weight；Nitrogen source is hair
Ferment culture medium weight 3-5% urea, fermentation medium weight 1-3% ammonium sulfate, fermentation medium weight 2-3% phosphoric acid
Diammonium hydrogen；Inorganic salts are fermentation medium weight 1-2% potassium sulfate, fermentation medium weight 3-5% calcium carbonate or mistake phosphorus
One kind, fermentation medium weight 0.5-1% magnesium sulfate in sour calcium；Organic matter is trash fish, meat mincing, grouts, two in wheat bran
Kind or three kinds, content be fermentation medium weight 25-40%.
4. utilize fermentation method production amino acid bio-organic fertilizer as described in claim 1, it is characterised in that：The fermentate exists
In the general fermentation tank of open type at 28-32 DEG C of PH4-6, temperature, 60-100rpm revolutions stirring or intermittent stirring under ferment training
Support.
5. utilize fermentation method production amino acid bio-organic fertilizer as described in claim 1, it is characterised in that：The organic fertilizer is made
To rush fertilising or fertilizer for spraying on leaf surface, available for field crop, in vegetables or fruit tree.
A kind of 6. preparation method that biological organic fertilizer is produced using fermentation method described in claim 1, it is characterised in that：Will be respectively
Domestication can extremely reach the mixed bacteria liquid being made up of saccharomycete and bacillus under the conditions of Co metabolism, be seeded in through high-temperature sterilization
The fermentation medium being made up of carbon source, nitrogen source, inorganic salts and organic matter in, using the general fermentation tank of open type, fermented
Culture 10-15 days, separation supernatant produce amino acid bio-organic fertilizer, wherein, saccharomycete is Candida lipolytica, and powdery is finished red
Yeast and S. cervisiae, bacillus are Bacillus subtillis and wax bacillus.
7. the preparation method that biological organic fertilizer is produced using fermentation method as described in claim 6, it is characterised in that：The fermentation
Carbon source is glucose in culture medium, sucrose, starch, one kind in molasses, and content is the 5-10% of fermentation medium weight；Nitrogen source
For fermentation medium weight 3-5% urea, fermentation medium weight 1-3% ammonium sulfate, fermentation medium weight 2-3%'s
Diammonium hydrogen phosphate；Inorganic salts be fermentation medium weight 1-2% potassium sulfate, fermentation medium weight 3-5% calcium carbonate or
One kind, fermentation medium weight 0.5-1% magnesium sulfate in calcium superphosphate；Organic matter is trash fish, meat mincing, grouts, wheat bran two
Kind or the 25-40% that three kinds of contents are fermentation medium weight.
8. the preparation method that biological organic fertilizer is produced using fermentation method as described in claim 6, it is characterised in that：The solution fat
Candida, pichia farinose, saccharomyces cerevisiae, Bacillus subtillis, wax bacillus is respectively in high salt, high sugar, and contain
What organic protein enriched, PH is that culture to normal growth and then realization is tamed in neutral culture medium, is then tamed and dociled above-mentioned respectively
Strain after change, then pure culture is carried out respectively, bacterium solution is according to yeast：Bacillus is with 1:1.5-2 volume ratios mix.
9. the preparation method that biological organic fertilizer is produced using fermentation method as described in claim 6 or 8, it is characterised in that：It is described
Candida lipolytica in yeast, pichia farinose, saccharomyces cerevisiae press 1:1:1 volume ratio mixing；
Bacillus subtillis in bacillus, wax bacillus press 1:1 volume ratio mixing.
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Cited By (5)
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| CN109180378A (en) * | 2018-09-15 | 2019-01-11 | 湖南泰谷生态工程有限公司 | A method of Amino acid liquid fertilizer is prepared using dead fish shrimp |
| CN109354521A (en) * | 2018-09-26 | 2019-02-19 | 广东省农业科学院农业资源与环境研究所 | A kind of Amino acid liquid fertilizer mother liquor and preparation method thereof |
| CN109438069A (en) * | 2018-12-13 | 2019-03-08 | 中山市承铭农业技术开发有限公司 | A method of utilizing sugar refinery leftover bits and pieces Amino acid liquid fertilizer |
| CN110683893A (en) * | 2019-10-09 | 2020-01-14 | 广西富凤农牧集团有限公司 | Chicken manure fermented fertilizer and preparation method thereof |
| CN113956078A (en) * | 2021-12-01 | 2022-01-21 | 曲靖金弘泰生物制品有限公司 | System and method for co-production of high-quality organic liquid fertilizer during production of bone particles, bone powder and bone oil |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109180378A (en) * | 2018-09-15 | 2019-01-11 | 湖南泰谷生态工程有限公司 | A method of Amino acid liquid fertilizer is prepared using dead fish shrimp |
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| CN109438069A (en) * | 2018-12-13 | 2019-03-08 | 中山市承铭农业技术开发有限公司 | A method of utilizing sugar refinery leftover bits and pieces Amino acid liquid fertilizer |
| CN110683893A (en) * | 2019-10-09 | 2020-01-14 | 广西富凤农牧集团有限公司 | Chicken manure fermented fertilizer and preparation method thereof |
| CN113956078A (en) * | 2021-12-01 | 2022-01-21 | 曲靖金弘泰生物制品有限公司 | System and method for co-production of high-quality organic liquid fertilizer during production of bone particles, bone powder and bone oil |
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Application publication date: 20180410 |