CN102428967A - Use and method for controlling diaphorina citri by using beauveria bassiana - Google Patents
Use and method for controlling diaphorina citri by using beauveria bassiana Download PDFInfo
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- CN102428967A CN102428967A CN2011103324738A CN201110332473A CN102428967A CN 102428967 A CN102428967 A CN 102428967A CN 2011103324738 A CN2011103324738 A CN 2011103324738A CN 201110332473 A CN201110332473 A CN 201110332473A CN 102428967 A CN102428967 A CN 102428967A
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- white muscardine
- muscardine fungi
- beauveria bassiana
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Abstract
The invention discloses a new use and a method for controlling diaphorina citri by using beauveria bassiana. The method provided by the invention comprises the following steps: firstly cultivating and producing beauveria bassiana; searching the parasitic psyllidae in the beauveria bassiana from citrus orchards; separating, cultivating, inoculating and selecting the beauveria bassiana through normal methods in a laboratory; cultivating and producing the selected beauveria bassiana to obtain the product, wherein the product can be prepared as beauveria bassiana powder or beauveria bassiana liquid. The beauveria bassiana powder or the beauveria bassiana liquid can be sprayed on orange trees. Or the method comprises the following steps: selecting and cultivating the beauveria bassiana which not only can hurt or kill the psyllidae, but also has low or no toxin for natural enemies, such as insects or mites; packaging the beauveria bassiana and the natural enemies which are the insects or the mites in packaging containers, such as releasing paper bags or bottles; hanging or nailing the packaging containers on the tree trunks to release in the citrus orchards, wherein the psyllidae is infected with the beauveria bassiana, then the beauveria bassiana is parasitic on the psyllidae, finally the psyllidae dies.
Description
Technical field
The present invention relates to utilize the purposes and the method for white muscardine fungi control citrus psylla.
Background technology
Citrus yellow shoot disease is one of destructive disease of tool on world citrus is produced, and after citrus is infected by yellow twig, not only produces the yellow tip, blade is mottled and symptom such as nutritional deficiency, also causes the variation of a series of cells physiological and pathology; Citrus yellow shoot disease mainly is to propagate through citrus psylla band " poison " body except through the graft transmission, the citrus psylla adult have efficient biography characteristic of disease, closely diffusibility, be with toxicity and pass characteristics such as characteristic of disease fast all the year round.In the world controlling citrus psylla as the most important technical measures of citrus yellow shoot disease integrated control, medicine is sprayed 1-2 time in generally clear in the winter time garden at present, when an annual spring, summer, autumn growth, sprays medicine once when young sprout was seen in the young leaves beginning at every turn, sprays medicine again after 10 days.1 year needs use agricultural chemicals 8-10 time, but have little effect, and contaminated environment seriously.Therefore seek the objective of the struggle that the biological control citrus psylla is a researcher always.The inventor has carried out the series of studies of utilizing white muscardine fungi control citrus psylla in research citrus psylla biological control process, through repetition test, obtained success, has solved a great problem that this area exists for a long time.
Summary of the invention
One of task of the present invention provides a kind of new purposes of utilizing white muscardine fungi control evil, promptly utilizes the application of white muscardine fungi control citrus psylla.
Two of task of the present invention provides the method for utilizing white muscardine fungi control citrus psylla, and it is realized through following technical scheme:
The present invention cultivates earlier and produces white muscardine fungi, can be made into the white muscardine fungi pulvis or processes the white muscardine fungi liquor.
In the wood louse emergence period, the white muscardine fungi pulvis is sprayed directly on the orange tree, the conidial powder of white muscardine fungi is sprayed directly on the particularly tender tip of plant of citrus citrus, perhaps the white muscardine fungi liquor is sprayed on the orange tree.
Also can select can kill and wound, kill wood louse again to natural enemy insect or mite class low toxicity or nontoxic white muscardine fungi is cultivated, this white muscardine fungi and natural enemy insect or mite class is packaging together; Be packaged in the packing containers such as discharging paper bag or bottle; Take the tangerine garden to; Hang or follow closely on the tree bar, release propagates in the citrus garden then.
Contrast with prior art; The present invention uses white muscardine fungi control citrus psylla and still belongs to the first at home and abroad; The present invention utilizes white muscardine fungi that citrus psylla is had very strong to infect, parasitic, the effect that causes death; Control citrus garden wood louse can reach reduction environment pesticide pollution, slow down the purpose that the yellow twig diffusion spreads.
Description of drawings
Fig. 1 inserts the change procedure photo in the PDA medium with the wood louse nymph of long white hypha and Si Shi genus amblyseius berlese.(explain: the mycelia that the predation mite grows on one's body is the same with the mycelia of wood louse nymph, in the time of the 3rd day, gets a fritter; Insert in another medium again and cultivate, in the time of the 4th day, this medium miospore powder is scattered to other places; Grow a lot of mycelia, other has contaminated, grows mold colony.)
Fig. 2 is the citrus psylla photo that not infected by white muscardine fungi;
Fig. 3 is the wood louse photo that infected by white muscardine fungi;
Fig. 4 is infected and the wood louse contrast photo that not infected by white muscardine fungi by white muscardine fungi;
Fig. 5 is the wood louse photo that infected by white muscardine fungi;
Fig. 6 receives white muscardine fungi to infect one of deadly citrus psylla nymph photo;
Fig. 7 receives white muscardine fungi to infect two of deadly citrus psylla nymph photo;
Fig. 8 receives white muscardine fungi to infect deadly citrus psylla photo;
Fig. 9 propagates white muscardine fungi to infect the citrus psylla photo after the pure peaceful mite of cucumber mixes with white muscardine fungi;
Figure 10 is the citrus psylla adult photo that infected by white muscardine fungi;
Figure 11 is to citrus psylla dissemination photo under the different situations.
Embodiment:
Below in conjunction with specific embodiment the present invention is further specified.(but not being limitation of the present invention).
The present invention at first will cultivate and produce white muscardine fungi; Can from the citrus garden, seek by the parasitic wood louse of white muscardine fungi; In the laboratory, separating, cultivate, inoculate, select white muscardine fungi according to the method for routine then cultivates, produces; Process product, product can be made into the white muscardine fungi pulvis or processes the white muscardine fungi liquor.
In the wood louse emergence period; The white muscardine fungi pulvis is sprayed directly on the orange tree, the conidial powder of white muscardine fungi is sprayed directly on the particularly tender tip of plant of citrus citrus, perhaps the white muscardine fungi liquor is sprayed on the orange tree; In order to infect citrus psylla, make citrus psylla parasitic and dead by white muscardine fungi.
Also can select can kill and wound, kill citrus psylla again to natural enemy insect or mite class low toxicity or nontoxic white muscardine fungi is cultivated, then that this white muscardine fungi and natural enemy insect or mite class is packaging together; Be packaged in the packing containers such as discharging paper bag or bottle; Taking the tangerine garden to hangs or follows closely on the tree bar; Release propagates in the citrus garden, and this white muscardine fungi is infected citrus psylla, makes it by parasitic dead.
Below photo accompanying drawing through test data and test come further summary feasible and useful effect of the present invention, and further specify the present invention, but be not limitation of the present invention.
Test Example: different disposal is studied the wood louse lethality rate
25±1℃
Experimental observation shows that white muscardine fungi has very strong pathogenic effects to wood louse, and the ovum of wood louse, nymph, adult all can be infected to cause death, shown in accompanying drawing.With infected wood louse in the test carry out once more separation and Culture carry out again subinfection still have very strong pathogenic, shown in accompanying drawing.
Claims (4)
1. utilize the application of white muscardine fungi control citrus psylla.
2. according to the described application of claim 1; It is characterized in that; The method of described application is: cultivate earlier and the production white muscardine fungi, from the citrus garden, seek by the parasitic wood louse of white muscardine fungi, in the laboratory, separate, cultivate, inoculate, select white muscardine fungi according to the method for routine then and cultivate, produce; Process the white muscardine fungi product, product is processed the white muscardine fungi pulvis or is processed the white muscardine fungi liquor.
3. according to the described application of claim 2, it is characterized in that, described white muscardine fungi pulvis is sprayed directly on the orange tree, the conidial powder of white muscardine fungi is sprayed directly on the particularly tender tip of plant of citrus citrus; Perhaps the white muscardine fungi liquor is sprayed on the orange tree,, makes citrus psylla parasitic and dead by white muscardine fungi in order to infect citrus psylla.
4. according to the described application of claim 1, it is characterized in that, select and to kill and wound, kill wood louse again to natural enemy insect or mite class low toxicity or nontoxic white muscardine fungi; Cultivate; Then that this white muscardine fungi and natural enemy insect or mite class is packaging together, be packaged in the packing containers such as discharging paper bag or bottle, take that the tangerine garden hangs to or nail on the tree bar; Release propagates in the citrus garden, infects wood louse and makes it by parasitic dead.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103324738A CN102428967A (en) | 2011-10-25 | 2011-10-25 | Use and method for controlling diaphorina citri by using beauveria bassiana |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103324738A CN102428967A (en) | 2011-10-25 | 2011-10-25 | Use and method for controlling diaphorina citri by using beauveria bassiana |
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| CN102428967A true CN102428967A (en) | 2012-05-02 |
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| CN2011103324738A Pending CN102428967A (en) | 2011-10-25 | 2011-10-25 | Use and method for controlling diaphorina citri by using beauveria bassiana |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103120105A (en) * | 2013-02-07 | 2013-05-29 | 广东省昆虫研究所 | Method for controlling diaphorina citri and citrus greening diseases in citrus orchard |
| CN106233980A (en) * | 2016-08-09 | 2016-12-21 | 胡永军 | A kind of method utilizing muscardine preventing and treating vegetable aleyrodid |
| CN108617696A (en) * | 2018-04-28 | 2018-10-09 | 广西青又青生物肥业有限公司 | A kind of complex micro organism fungicide for Citrus Huanglongbing pathogen and preparation method thereof and the Citrus Huanglongbing pathogen special biological agent prepared with it |
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| CN1730647A (en) * | 2005-08-23 | 2006-02-08 | 浙江大学 | Bacterial strain and its formulation for preventiong sting type trophi insect |
| CN101002572A (en) * | 2007-01-16 | 2007-07-25 | 张艳璇 | Method for controlling insects by using entomogenous fungi carried by predation-mite |
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Patent Citations (2)
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| CN1730647A (en) * | 2005-08-23 | 2006-02-08 | 浙江大学 | Bacterial strain and its formulation for preventiong sting type trophi insect |
| CN101002572A (en) * | 2007-01-16 | 2007-07-25 | 张艳璇 | Method for controlling insects by using entomogenous fungi carried by predation-mite |
Non-Patent Citations (4)
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| 《福建农业科技》 20101231 张艳璇等 "以螨治螨"防治柑桔黄龙病的效果与原因初探 第60-61页 1-4 , 第6期 * |
| 张艳璇等: ""以螨治螨"防治柑桔黄龙病的效果与原因初探", 《福建农业科技》, no. 6, 31 December 2010 (2010-12-31), pages 60 - 61 * |
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| 章玉苹等: "柑桔木虱的生物防治研究进展", 《中国生物防治》, vol. 25, no. 2, 31 May 2009 (2009-05-31) * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103120105A (en) * | 2013-02-07 | 2013-05-29 | 广东省昆虫研究所 | Method for controlling diaphorina citri and citrus greening diseases in citrus orchard |
| CN103120105B (en) * | 2013-02-07 | 2014-12-10 | 广东省昆虫研究所 | Method for controlling diaphorina citri and citrus greening diseases in citrus orchard |
| CN106233980A (en) * | 2016-08-09 | 2016-12-21 | 胡永军 | A kind of method utilizing muscardine preventing and treating vegetable aleyrodid |
| CN108617696A (en) * | 2018-04-28 | 2018-10-09 | 广西青又青生物肥业有限公司 | A kind of complex micro organism fungicide for Citrus Huanglongbing pathogen and preparation method thereof and the Citrus Huanglongbing pathogen special biological agent prepared with it |
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Application publication date: 20120502 |