CN109526547A - A kind of method of bottle of cultivation yellow needle mushroom - Google Patents
A kind of method of bottle of cultivation yellow needle mushroom Download PDFInfo
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- CN109526547A CN109526547A CN201811399603.8A CN201811399603A CN109526547A CN 109526547 A CN109526547 A CN 109526547A CN 201811399603 A CN201811399603 A CN 201811399603A CN 109526547 A CN109526547 A CN 109526547A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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Abstract
The method for planting yellow needle mushroom the present invention provides a kind of bottle, it comprises the following components in parts by weight: 10-15 parts of lemon peel, 5-8 parts of sugarcane, 5-10 parts of hotbed chives, 3-6 parts of millet flour, 3-6 parts of pumpkin, 0.5-1 parts of sodium chloride, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, 0.5-1 parts of tomato extracting solution, 0.3-0.5 parts of paclobutrazol powder, 0.2-0.4 parts of drop-proof agent powder, 3-5 parts of chitosan, 0.5-2.5 parts of magnesium carbonate, 20-25 parts of trehalose, 1.3-9.5 parts of nanometer calcium carbonate, 0.2-0.8 parts of sodium lauryl sulfonate.The method that bottle provided by the invention plants yellow needle mushroom, by using hotbed chives, hotbed chives contain protein abundant, sugar, mineral calcium, iron and phosphorus, provitamin A, vitamin B2, vitamin c and niacin and glucoside and amaroid etc., the stasis of blood is dissipated with warding off the cold, the effect of reinforcement power, and can improve a poor appetite.
Description
Technical field
The present invention relates to edible mushroom culture technique field, the method for planting yellow needle mushroom in particular to a kind of bottle.
Background technique
Needle mushroom scientific name hair handle money bacterium, also known as hair handle small fire mushroom, plain mushroom, dried mushroom, plain wild rice, freeze bacterium, golden mushroom, intelligence at structure bacterium
Power mushroom etc..Because its stem is elongated, like day lily, therefore claims needle mushroom, belong to Agaricales Tricholomataceae needle gold mushroom category, be a kind of bacterium algae lichens
Class.Needle mushroom has very high medicinal dietary function.
Needle mushroom is both a kind of ticbit and preferable health food, and the domestic and international market of needle mushroom is increasingly wide.
Needle mushroom artificial cultivation technique is simultaneously uncomplicated, as long as can control environmental condition, is easy for obtaining reliable and stable yield.According to survey
Fixed, the content of needle mushroom amino acid is very rich, is higher than general mushroom class, reaches in every 100g fresh mushroom containing total amino acid content
20.9mg, wherein 8 kinds of essential amino acids account for the 42.29~51.17% of total amount, isoleucine and content of glutamic acid highest,
Arginine and lysine content are higher in essential amino acid, and the arginine of high level can prevent and treat hepatitis, stomach and intestine feedback disease
Etc. disease of digestive systems, lysine can promote upgrowth and development of children, enhancing memory improves intelligence.Contain egg in needle mushroom dry product
White matter 8.87%, carbohydrate 60.2%, crude fibre are often edible to prevent and treat canker up to 7.4%.But it is normal using this field
Under the premise of rule culture medium, conventional culture methods are cultivated, the content of above-mentioned nutritional ingredient is difficult to increase again.
Yellow needle mushroom have the speed of growth is fast, disease resistance is strong, thermal adaptability is strong, mushroom taste is denseer, mouthfeel is more preferable, but its
Have the shortcomings that mushroom type is not full, mushroom body is soft, mushroom foot color is deep.
Meanwhile needle mushroom post harvest transport major physiological Biochemical changes include Tissue Browning, cell wall protein and polysaccharide drop
Solution, fructification aging etc., these variations have seriously affected needle mushroom quality.
Summary of the invention
The present invention is directed to overcome the shortcomings of in existing needle mushroom production and Cultivating techniques, a kind of bottle of cultivation yellow gold is proposed
The method of needle mushroom.
The method for planting yellow needle mushroom the invention proposes a kind of bottle, includes the following steps:
Step a: after choosing Fresh Lemon peeling, each lemon peel sheet weight is between 2-3g, in 3% sodium chloride solution
Middle immersion 3-4d, dries, is pulverized into powder, spare；
Step b: choosing fresh hotbed chives, is cut to the segment that length is 0.5-1cm, mixed with above-mentioned powdered lemon peel
It closes, stirs evenly, add the water of 10 times of weight, at 25-40 DEG C at a temperature of edging up, vibrate, stand；
Step c: choosing without insect pest, disease-free sugarcane, and the diameter of every sugarcane is 2-2.5cm, length 2.2-2.5m,
After clean the surface, it is cut into the uniform cylindrical shape dissection that size is 2-3cm, after wrapping up with reverse osmosis membrane, in 1% Betagen Solution
Middle immersion 2-4 hours；Dimension life is injected into sugarcane with the method for immersion and syringe injection respectively during plantation and after harvest
Plain A and vitamin E mixed liquor 20-40mL；
Step d: by the lemon peel of above-mentioned preparation and hotbed chives mixed liquor, sugarcane, millet flour, pumpkin, sodium chloride, dried silkworm chrysalis meal,
Yeast powder, tomato extracting solution, paclobutrazol powder, drop-proof agent powder, chitosan, magnesium carbonate, trehalose, nanometer calcium carbonate, laruyl alcohol sulphur
Sour sodium, according to parts by weight, the continuous heating in 75-85 DEG C of water-bath, the drop-proof agent powder addition institute of paclobutrazol powder and parts by weight
It states in aqueous solution, and continues heating water bath until the paclobutrazol powder and the drop-proof agent powder are dissolved completely in the aqueous solution
In, ultrasonic vibration 5-10min stands 10-12 hours, obtains culture medium stoste；
In above-mentioned steps b, Vltrasonic device is vibrated according to the relationship of following ultrasonic vibration frequencies and heating temperature,
Wherein, in the first temperature section, in 25-40 DEG C of temperature range:
In formula, f1Indicate the real-time vibration frequency of the first temperature section, T indicates the real time temperature in the first temperature section, T0It indicates
With reference to preset temperature value, temperature is 30 DEG C, and m indicates the hotbed chives weight in the mixed liquor being added, and M is indicated in the mixed liquor being added
The quality of lemon peel, c indicate the specific heat of mixed liquor, f0The default vibration frequency for indicating the first temperature section, is 25kHz；
Wherein, in second temperature section, in 40-45 DEG C of temperature range:
In formula, f2Indicate the real-time vibration frequency of second temperature section, T indicates the real time temperature in second temperature section, T10Table
Show that, with reference to preset temperature value, thermostat temperature is 40 DEG C, m indicates the hotbed chives weight in the mixed liquor being added, and M indicates the mixing being added
The quality of lemon peel in liquid, c indicate the specific heat of mixed liquor, f10Indicate second temperature section default vibration frequency, be
55kHz；
In above-mentioned steps d, it is 25kHz that room temperature, which is warming up to the vibration frequency in 45 DEG C of temperature ranges,；
When being warming up to 45 DEG C, in 45-55 DEG C of temperature range:
In formula, f3Indicate the real-time vibration frequency of third temperature section, T indicates the real time temperature in third temperature section, T20Table
Show that, with reference to preset temperature value, temperature is 45 DEG C, and c indicates the specific heat of mixed liquor, f20Indicate the default vibration frequency of the first temperature section,
It is 40kHz；
Wherein, in temperature-fall period, in 25-55 DEG C of temperature range:
In formula, f4Indicate the real-time vibration frequency of the 4th temperature section, T indicates that the real time temperature in the 4th temperature section, c indicate
The specific heat of mixed liquor, m indicate the hotbed chives weight in the mixed liquor being added, and M indicates total matter of the lemon peel in the mixed liquor being added
Amount, f30The default vibration frequency for indicating the 4th temperature section is 30kHz；
Step e: the matrix that step d is made is packed into bacterium bottle, and the matrix during bottling in each bacterium bottle will uniformly, week
Enclose tight, Weight control in 1.6-2.0Kg, bacterium it is bottled it is good after, bottleneck and its surrounding are cleaned out, sealing is put into equipment
In at 100 DEG C constant temperature sterilize 12-18h, after sterilizing bacterium bottle move into inoculated and cultured room in, to bacterium bottle temperature drop to 25 DEG C with
Under；
Step f: Spawn incubation: gained bacterium bottle in step e is suspended in culturing room, keeping culture room temperature is 20-25
DEG C, air humidity≤75% opens the comprehensive irradiation bacterium bottle 10-20min of ultraviolet light every 3-5h, while being aided with infrared spoke
It penetrates, until mycelia sends out full bottle bacterium, mycelia is exposed and receives sunlight according to solarization, and discontinuity is sprayed water, until needle mushroom is long greatly
Only；
Step g: timely collecting: picking mature needle mushroom, adopted first batch of needle mushroom, by the u-turn up and down of bacterium bottle
It puts, and fills the water, continue to pick when needle mushroom grows up to.
Further, the culture medium stoste comprises the following components in parts by weight: 10-15 parts of lemon peel, 5-8 parts of sugarcane,
5-10 parts of hotbed chives, 3-6 parts of millet flour, 3-6 parts of pumpkin, 0.5-1 parts of sodium chloride, 2-3 parts of dried silkworm chrysalis meal, 0.3-0.5 parts of yeast powder, kind
0.5-1 parts of eggplant extracting solution, 0.3-0.5 parts of paclobutrazol powder, 0.2-0.4 parts of drop-proof agent powder, 3-5 parts of chitosan, magnesium carbonate 0.5-2.5
Part, 20-25 parts of trehalose, 1.3-9.5 parts of nanometer calcium carbonate, 0.2-0.8 parts of sodium lauryl sulfonate.
Further, in the production process, after choosing Fresh Lemon peeling, each lemon peel sheet weight exists the lemon peel
Between 2-3g, 3-4d is impregnated in 3% sodium chloride solution, is dried, is pulverized into powder, it is spare.
Further, in the manufacturing process of the hotbed chives, fresh hotbed chives are chosen, being cut to length is the small of 0.5-1cm
Section, mixes with above-mentioned powdered lemon peel part, stirs evenly, add the water of 10 times of weight, at 25-40 DEG C at a temperature of edging up,
Oscillation is stood.
Further, it in the manufacturing process of sugarcane, chooses without insect pest, disease-free, without edge of a knife damage sugarcane, every sweet
The diameter of sugarcane is 2-2.5cm, length 2.2-2.5m, after clean the surface, is cut into the uniform cylindrical shape dissection that size is 2-3cm,
After being wrapped up with reverse osmosis membrane, impregnated 2-4 hours in 1% Betagen Solution；Respectively with immersion during plantation and after harvest
Vitamin A and vitamin E mixed liquor 20-40mL are injected into sugarcane with the method for syringe injection.
Further, in planting process, respectively a third after growing cane month, the 6th month and nine month into
Row one injection, injection point are sugarcane root, injection volume 2-4mL；Another kind is each sugarcane after sugarcane maturation dissection
Four injection points are circumferentially uniformly chosen in the middle position of dissection, inject vitamin A and 3 3- of vitamin E respectively
4mL injects vitamin A and vitamin e1 03mL-15mL at the both ends cut surface center of billets of cane, later in notch respectively
Surface uniformly applies berley salt 2-3g, and covers entire billets of cane with preservative film, stands 2-4 hours, is added in pulverizer, after crushing
Sugarcane clast, the vitamin A and vitamin E ethyl acetate solution for being 5% with concentration impregnate 3-5 hours, pull out, dry, be made
Processed sugarcane.
Further, in above-mentioned steps a, the millet flour, pumpkin, bean cake powder, dried silkworm chrysalis meal, yeast powder granularity be
60-80 mesh.
Further, during above-mentioned Spawn incubation, the UV illumination intensity is 2-3.5 μ w/cm2。
Further, during above-mentioned Spawn incubation, the yellow water that bacteria stick spues need to be rinsed well when water spray in time, is prevented
Yellow water condenses in bacteria stick；During above-mentioned Spawn incubation, mycelia needs to carry out that ear is urged to handle to mycelia after growing；Urge ear process
Middle daytime covers bacterium bottle with plastic film and is kept the temperature, and controls its temperature≤28 DEG C, night opens plastic film cooling, continuous to locate
Reason 5-7 days.
Compared with prior art, the beneficial effects of the present invention are, the method that bottle provided by the invention plants yellow needle mushroom,
By using hotbed chives, hotbed chives contain protein abundant, sugar, mineral calcium, iron and phosphorus, provitamin A, vitamin B2, vitamin c
And niacin and glucoside and amaroid etc., have to ward off the cold and dissipates the stasis of blood, the effect of reinforcement power, and can improve a poor appetite.By using this
The yellow needle mushroom cultivated is invented, amino acid content is abundant, and containing the bright height of sugar, growth strain is long.
The present invention uses lemon peel, and lemon peel contains citrin, tannin and vitamin A, B, C, uses hotbed chives and lemon at the same time
After lemon skin, powdered lemon peel is mixed with hotbed chives, is stirred evenly, and adds the water of 10 times of weight, in 25-40 DEG C of the temperature that edges up
Under, guarantee that multivitamin therein can be mutually mixed, reaches optimum efficiency；And by under progressive lower temperature environments with
The control of vibration frequency keep the various nutritional ingredients of the two material can, and drop various vitamins mixing.The present invention exists
The different phase of sugarcane is cultivated using sodium chloride, the addition of treated sugarcane, it is ensured that nutrition needed for plant growth
Ingredient, thus there is remarkable result in terms of volume increase, pest-resistant, sterilizing.
Further, the present invention is on the one hand Needle mushroom strain by the constituent and content of change fluid nutrient medium
Development provide sufficient nutrient, it is ensured that the quick breeding of Needle mushroom strain；Another side avoids Needle mushroom strain rear
Space-pollution in phase seeded process, the lysine and arginine content in needle mushroom after simultaneously effective improving plant, pole
The earth improves the mouthfeel of needle mushroom.
In particular, rationally controlling supersonic frequency and vibration frequency, on the one hand when present component and hotbed chives are combined
It combines various vitamins sufficiently and reaches optimal efficacy of adsorption；On the other hand, by the mixing vibration of Multiple components such as hotbed chives,
Nutritional ingredient is accumulated in into outer layer, needle mushroom facilitates absorption nutrient.In the first temperature section, the mixing of room temperature and slightly above room temperature
In liquid, increase vibration frequency；Meanwhile vibration frequency increases with the increase of specific gravity shared by hotbed chives, in the mistake of second stage
Cheng Zhong, as temperature increases, vibration frequency is slowly increased, and in 45 DEG C of temperature, reaches the highest point of vibration frequency, is being suitable for
Temperature and vibration frequency under, various nutritional ingredients are organically blended.
Further, bottle provided by the invention is planted in the method for yellow needle mushroom, by carrying out ultrasonication to culture medium,
Make each component in culture medium has arrived sufficient mixing, ensure that the nutriment uniformity at each position in culture medium, in turn
The homogeneity that nutriment distributes in later period Needle mushroom strain growth course is effectively ensured, golden mushroom is further ensured
The synchronism of kind growth.
Specific embodiment
Below with reference to specific embodiment, the technical scheme of the present invention will be further described, but claimed range is simultaneously
It is not limited to this.
The embodiment of the present invention proposes the method for a kind of bottle of cultivation yellow needle mushroom, comprises the following components in parts by weight: lemon
10-15 parts of skin, 5-8 parts of sugarcane, 5-10 parts of hotbed chives, 3-6 parts of millet flour, 3-6 parts of pumpkin, 0.5-1 parts of sodium chloride, dried silkworm chrysalis meal 2-3
Part, 0.3-0.5 parts of yeast powder, 0.5-1 parts of tomato extracting solution, 0.3-0.5 parts of paclobutrazol powder, 0.2-0.4 parts of drop-proof agent powder, shell are poly-
3-5 parts sugared, 0.5-2.5 parts of magnesium carbonate, 20-25 parts of trehalose, 1.3-9.5 parts of nanometer calcium carbonate, sodium lauryl sulfonate 0.2-0.8
Part.
The manufacturing process of above-mentioned culture medium are as follows:
Step a: after choosing Fresh Lemon peeling, each lemon peel sheet weight is between 2-3g, in 3% sodium chloride solution
Middle immersion 3-4d, dries, is pulverized into powder, spare；
Step b: choosing fresh hotbed chives, is cut to the segment that length is 0.5-1cm, mixed with above-mentioned powdered lemon peel part
It closes, stirs evenly, add the water of 10 times of weight, at 25-40 DEG C at a temperature of edging up, vibrate, stand；
Step c: choosing without insect pest, disease-free sugarcane, and the diameter of every sugarcane is 2-2.5cm, length 2.2-2.5m,
After clean the surface, it is cut into the uniform cylindrical shape dissection that size is 2-3cm, after wrapping up with reverse osmosis membrane, in 1% Betagen Solution
Middle immersion 2-4 hours；Dimension life is injected into sugarcane with the method for immersion and syringe injection respectively during plantation and after harvest
Plain A and vitamin E mixed liquor 20-40mL；In planting process, respectively a third after growing cane month, the 6th month and
Progress one injection in nine month, injection point are sugarcane root, injection volume 2-4mL；Another kind be after sugarcane maturation dissection,
Four injection points are circumferentially uniformly chosen in the middle position of each sugarcane dissection, inject vitamin A and Wei Sheng respectively
Plain E3 3-4mL injects 0 3mL-15mL of vitamin A and vitamin e1 respectively at the both ends cut surface center of billets of cane, it
Berley salt 2-3g is uniformly applied in cut surface afterwards, and entire billets of cane is covered with preservative film, 2-4 hours is stood, pulverizer is added
In, sugarcane clast after crushing, the vitamin A and vitamin E ethyl acetate solution for being 5% with concentration impregnates 3-5 hours, it pulls out,
It dries, processed sugarcane is made.
Step d: by the lemon peel of above-mentioned preparation and hotbed chives mixed liquor, sugarcane, millet flour, pumpkin, sodium chloride, dried silkworm chrysalis meal,
Yeast powder, tomato extracting solution, paclobutrazol powder, drop-proof agent powder, chitosan, magnesium carbonate, trehalose, nanometer calcium carbonate, laruyl alcohol sulphur
Sour sodium, according to parts by weight, the continuous heating in 75-85 DEG C of water-bath, the drop-proof agent powder addition institute of paclobutrazol powder and parts by weight
It states in aqueous solution, and continues heating water bath until the paclobutrazol powder and the drop-proof agent powder are dissolved completely in the aqueous solution
In, ultrasonic vibration 5-10min stands 10-12 hours, obtains culture medium stoste；
In above-mentioned steps b, Vltrasonic device is vibrated according to the relationship of following ultrasonic vibration frequencies and heating temperature,
Wherein, in the first temperature section, in 25-40 DEG C of temperature range:
In formula, f1Indicate the real-time vibration frequency of the first temperature section, T indicates the real time temperature in the first temperature section, T0It indicates
With reference to preset temperature value, temperature is 30 DEG C, and m indicates the hotbed chives weight in the mixed liquor being added, and M is indicated in the mixed liquor being added
The quality of lemon peel, c indicate the specific heat of mixed liquor, f0The default vibration frequency for indicating the first temperature section, is 25kHz；
Wherein, in second temperature section, in 40-45 DEG C of temperature range:
In formula, f2Indicate the real-time vibration frequency of second temperature section, T indicates the real time temperature in second temperature section, T10Table
Show that, with reference to preset temperature value, thermostat temperature is 40 DEG C, m indicates the hotbed chives weight in the mixed liquor being added, and M indicates the mixing being added
The quality of lemon peel in liquid, c indicate the specific heat of mixed liquor, f10Indicate second temperature section default vibration frequency, be
55kHz；
In above-mentioned steps d, it is 25kHz that room temperature, which is warming up to the vibration frequency in 45 DEG C of temperature ranges,；
When being warming up to 45 DEG C, in 45-55 DEG C of temperature range:
In formula, f3Indicate the real-time vibration frequency of third temperature section, T indicates the real time temperature in third temperature section, T20Table
Show that, with reference to preset temperature value, temperature is 45 DEG C, and c indicates the specific heat of mixed liquor, f20Indicate the default vibration frequency of the first temperature section,
It is 40kHz；
Wherein, in temperature-fall period, in 25-55 DEG C of temperature range:
In formula, f4Indicate the real-time vibration frequency of the 4th temperature section, T indicates that the real time temperature in the 4th temperature section, c indicate
The specific heat of mixed liquor, m indicate the hotbed chives weight in the mixed liquor being added, and M indicates total matter of the lemon peel in the mixed liquor being added
Amount, f30The default vibration frequency for indicating the 4th temperature section is 30kHz.
Step e: the matrix that step d is made is packed into bacterium bottle, and the matrix during bottling in each bacterium bottle will uniformly, week
Enclose tight, Weight control in 1.6-2.0Kg, bacterium it is bottled it is good after, bottleneck and its surrounding are cleaned out, sealing is put into equipment
In at 100 DEG C constant temperature sterilize 12-18h, after sterilizing bacterium bottle move into inoculated and cultured room in, to bacterium bottle temperature drop to 25 DEG C with
Under；
Step f: Spawn incubation: gained bacterium bottle in step e is suspended in culturing room, keeping culture room temperature is 20-25
DEG C, air humidity≤75% opens the comprehensive irradiation bacterium bottle 10-20min of ultraviolet light every 3-5h, while being aided with infrared spoke
It penetrates, until mycelia sends out full bottle bacterium, mycelia is exposed and receives sunlight according to solarization, and discontinuity is sprayed water, until needle mushroom is long greatly
Only；
It further include the first bright sensor, to described first specifically, the first ultraviolet lamp group is arranged in culturing room
Brightness near ultraviolet lamp group is detected, and be will test result and be transmitted in control unit, and control unit is according to detection
Natural light situation determines the light levels of the first ultraviolet lamp group, further includes the first spectrum sensor, to first ultraviolet lamp
Spectrum near group is detected, and is transmitted in control unit, and control unit divides nature according to the wave-length coverage of detection spectrum
Light and artificial light, and it is ultraviolet with the brightness progress operation acquisition first near the first ultraviolet lamp group of the described first ultraviolet lamp group
Natural light brightness situation near lamp group.
In the present invention, indoors due to lamp group setting, if detecting real-time brightness case, its natural light can not rule out
Brightness, if energy-saving light can not be played according to the effect cultivated according to the brightness of brightness adjustment lamp group in environment at this time.Cause
This, is arranged a selecting module in the embodiment of the present invention, in control unit, the brightness detected for receiving all lamp groups, and with
The spectral information operation of first spectrum sensor acquisition obtains the brightness of natural light.
Specifically, the described first bright sensor obtains the brightness values L near the first ultraviolet lamp group in real time, mould is selected
Block obtains the real time spectrum information of the first spectrum sensor acquisition, and the wave-length coverage that the selecting module obtains the spectrum of acquisition is
A-D, and it is preset in selecting module the wave-length coverage B-C of lamp group, and determine the maximum value λ of acquisition wavelength1And it is minimum
Value λ2, to determine the SIN function of wave-length coverage Yu wavelength value λ:
λ (t)=λ2+sin(wt+k) (1)
In formula, λ indicates wavelength value, and t indicates wave-length coverage value, and value is from A-D, and when t value is A or D, λ value is λ2,
It is in t valueWhen, λ value is λ1, k expression constant, when determining SIN function relational expression, constant value be can determine, w takes
Value is either constant, and value is 1 in the present embodiment.
Based on the model that above-mentioned SIN function determines, natural light brightness coefficient a is determined1。
Natural light brightness value L near first ultraviolet lamp group1。
L1=L × a1 (3)
In formula, L indicates that the described first bright sensor obtains the brightness values near the first ultraviolet lamp group, a in real time1For certainly
Right coefficient of brightness.
The present invention passes through the determination of nature backscatter extinction logarithmic ratio, the influence of other light is excluded, by the spectrum of natural light and artificial light, root
Sinusoidal model is determined according to wavelength, and by the integral operation to sine, namely seek in SIN function, wave-length coverage A-B, with
And the sum of area of C-D accounts for entire area ratio of the wave-length coverage A-D based on SIN function, medium wavelength range B-C is artificial
The attainable range of light institute can specifically be set according to the use of lighting device.It is determined and is adjusted according to above-mentioned calculating process
The intensity of illumination of lamp group, yellow needle mushroom reach the most suitable illumination degree of nurturing.
Step g: timely collecting: picking mature needle mushroom, adopted first batch of needle mushroom, by the u-turn up and down of bacterium bottle
It puts, and fills the water, continue to pick when needle mushroom grows up to.
Wherein, in above-mentioned steps a, the millet flour, pumpkin, bean cake powder, dried silkworm chrysalis meal, yeast powder granularity be 60-
80 mesh.
Wherein, during above-mentioned Spawn incubation, the UV illumination intensity is 2-3.5 μ w/cm2。
Wherein, during above-mentioned Spawn incubation, the yellow water that bacteria stick spues need to be rinsed well when water spray in time, prevents yellow water
It is condensed in bacteria stick；During above-mentioned Spawn incubation, mycelia needs to carry out that ear is urged to handle to mycelia after growing；It urges white during ear
It is kept the temperature with plastic film covering bacterium bottle, controls its temperature≤28 DEG C, and night opens plastic film cooling, continuous processing 5-
7 days.
Embodiment one
The present embodiment mentions culture medium and is prepared from the following parts by weight of the components:
10 parts of lemon peel, 5 parts of sugarcane, 5 parts of hotbed chives, 3 parts of millet flour, 3 parts of pumpkin, 0.5 part of sodium chloride, 2 parts of dried silkworm chrysalis meal, ferment
0.3 part of female powder, 0.5 part of tomato extracting solution, 0.3 part of paclobutrazol powder, 0.2 part of drop-proof agent powder, 3 parts of chitosan, 0.5 part of magnesium carbonate,
20 parts of trehalose, 1.3 parts of nanometer calcium carbonate, 0.2 part of sodium lauryl sulfonate.
Culture medium is made according to above-mentioned culture medium manufacturing process.
Each lemon peel sheet weight is impregnated 3-4d in 3% sodium chloride solution, is dried, be crushed into powder between 2-3g
Last shape, it is spare；
Hotbed chives are cut to the segment that length is 0.5-1cm, mix with above-mentioned powdered lemon peel, stir evenly, add 10
The water of times weight at 25-40 DEG C at a temperature of edging up, is vibrated, is stood；
Third month after growing cane, the 6th month and progress one injection in nine month, injection point are sugarcane root
Portion, injection volume 2-4mL；After sugarcane is mature, choose without insect pest, disease-free sugarcane, the diameter of every sugarcane is 2-
2.5cm, length 2.2-2.5m after clean the surface, are cut into the uniform cylindrical shape dissection that size is 2-3cm, with reverse osmosis membrane packet
After wrapping up in, impregnated 2-4 hours in 1% Betagen Solution；After sugarcane maturation dissection, the interposition of each sugarcane dissection
It sets, circumferentially uniformly chooses four injection points, vitamin A and 3 3-4mL of vitamin E are injected respectively, in billets of cane
0 3mL-15mL of vitamin A and vitamin e1 is injected at the cut surface center of both ends respectively, uniformly applies spread in cut surface later
Salt 2-3g, and entire billets of cane is covered with preservative film, 2-4 hours are stood, is added in pulverizer, sugarcane clast after crushing, with
The vitamin A and vitamin E ethyl acetate solution that concentration is 5% impregnate 3-5 hours, pull out, dry, are made processed sweet
Sugarcane.
By the lemon peel of above-mentioned preparation and hotbed chives mixed liquor, sugarcane, millet flour, pumpkin, sodium chloride, dried silkworm chrysalis meal, yeast powder,
Tomato extracting solution, paclobutrazol powder, drop-proof agent powder, chitosan, magnesium carbonate, trehalose, nanometer calcium carbonate, sodium lauryl sulfonate are pressed
According to parts by weight, the continuous heating in 75-85 DEG C of water-bath, the drop-proof agent powder addition aqueous solution of paclobutrazol powder and parts by weight
In, and continuing heating water bath until the paclobutrazol powder and the drop-proof agent powder are dissolved completely in the aqueous solution, ultrasound is shaken
Dynamic 5-10min, stands 10-12 hours, obtains culture medium stoste.
The matrix made is packed into bacterium bottle, and the matrix during bottling in each bacterium bottle will uniformly, and surrounding tight is heavy
Amount control in 1.6-2.0Kg, bacterium it is bottled it is good after, bottleneck and its surrounding are cleaned out, sealing is put into equipment at 100 DEG C permanent
Temperature sterilizing 12-18h, the bacterium bottle after sterilizing move into inoculated and cultured room, drop to 25 DEG C or less to bacterium bottle temperature；
Spawn incubation: gained bacterium bottle in step e is suspended in culturing room, keeping culture room temperature is 20-25 DEG C, air
Humidity≤75% opens the comprehensive irradiation bacterium bottle 10-20min of ultraviolet light every 3-5h, while being aided with infra-red radiation, until bacterium
Silk sends out full bottle bacterium, and mycelia is exposed and receives sunlight according to solarization, and discontinuity is sprayed water, until needle mushroom is grown up；
Timely collecting: picking mature needle mushroom, adopted first batch of needle mushroom, and by bacterium bottle, u-turn is put up and down,
And fill the water, continue to pick when needle mushroom grows up to.
Embodiment two
Culture medium comprises the following components in parts by weight: 15 parts of lemon peel, 8 parts of sugarcane, 10 parts of hotbed chives, 6 parts of millet flour, south
6 parts of melon, 1 part of sodium chloride, 3 parts of dried silkworm chrysalis meal, 0.5 part of yeast powder, 1 part of tomato extracting solution, 0.5 part of paclobutrazol powder, drop-proof agent powder 0.4
Part, 5 parts of chitosan, 2.5 parts of magnesium carbonate, 25 parts of trehalose, 9.5 parts of nanometer calcium carbonate, 0.8 part of sodium lauryl sulfonate.
It is prepared according to one mode of above-described embodiment.
Embodiment three
Culture medium comprises the following components in parts by weight: 12 parts of lemon peel, 6 parts of sugarcane, 8 parts of hotbed chives, 5 parts of millet flour, pumpkin
5 parts, 0.8 part of sodium chloride, 2.5 parts of dried silkworm chrysalis meal, 0.4 part of yeast powder, 0.8 part of tomato extracting solution, 0.4 part of paclobutrazol powder, drop-proof agent
0.3 part of powder, 3.5 parts of chitosan, 2 parts of magnesium carbonate, 22 parts of trehalose, 6 parts of nanometer calcium carbonate, 0.5 part of sodium lauryl sulfonate.
Example IV
Culture medium comprises the following components in parts by weight: 14 parts of lemon peel, 7 parts of sugarcane, 6 parts of hotbed chives, 4 parts of millet flour, pumpkin
4 parts, it is 0.3 part of sodium chloride, 2.3 parts of dried silkworm chrysalis meal, 0.45 part of yeast powder, 0.6 part of tomato extracting solution, 0.35 part of paclobutrazol powder, anti-fall
0.355 part of plain powder, 3.6 parts of chitosan, 1.5 parts of magnesium carbonate, 23 parts of trehalose, 5 parts of nanometer calcium carbonate, sodium lauryl sulfonate 0.6
Part.
Reference examples
Culture medium comprises the following components in parts by weight: 5 parts of millet flour, 5 parts of pumpkin, 0.8 part of sodium chloride, dried silkworm chrysalis meal 2.5
Part, 0.4 part of yeast powder, 0.8 part of tomato extracting solution, 0.4 part of paclobutrazol powder, 0.3 part of drop-proof agent powder, 3.5 parts of chitosan, magnesium carbonate
2 parts, 22 parts of trehalose, 6 parts of nanometer calcium carbonate, 0.5 part of sodium lauryl sulfonate.
After Needle mushroom strain culture in above each formula, takes out 50 circles at random respectively and measure, measurement result mean value
It is as follows:
Obviously, the method that bottle provided by the invention plants yellow needle mushroom, by change fluid nutrient medium constituent and
On the one hand content provides sufficient nutrient for the development of Needle mushroom strain, greatly improves the yield of needle mushroom, effectively
The content of glutamic acid in needle mushroom after improving plant, significantly improves the mouthfeel of needle mushroom.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
Mind and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (9)
1. the method for a kind of bottle of cultivation yellow needle mushroom, which comprises the steps of:
Step a: after choosing Fresh Lemon peeling, each lemon peel sheet weight is soaked in 3% sodium chloride solution between 2-3g
3-4d is steeped, dries, is pulverized into powder, it is spare；
Step b: choosing fresh hotbed chives, is cut to the segment that length is 0.5-1cm, mixes, stir with above-mentioned powdered lemon peel
It mixes uniformly, adds the water of 10 times of weight, at 25-40 DEG C at a temperature of edging up, vibrate, stand；
Step c: it chooses without insect pest, disease-free sugarcane, the diameter of every sugarcane is 2-2.5cm, length 2.2-2.5m, cleaning
Behind surface, it is cut into the uniform cylindrical shape dissection that size is 2-3cm, after being wrapped up with reverse osmosis membrane, is soaked in 1% Betagen Solution
Bubble 2-4 hours；During plantation and harvest after respectively with impregnate and syringe injection method inject into sugarcane vitamin A with
Vitamin E mixed liquor 20-40mL；
Step d: by the lemon peel of above-mentioned preparation and hotbed chives mixed liquor, sugarcane, millet flour, pumpkin, sodium chloride, dried silkworm chrysalis meal, yeast
Powder, tomato extracting solution, paclobutrazol powder, drop-proof agent powder, chitosan, magnesium carbonate, trehalose, nanometer calcium carbonate, sodium lauryl sulfonate,
According to parts by weight, the drop-proof agent powder addition of the continuous heating in 75-85 DEG C of water-bath, paclobutrazol powder and parts by weight is described water-soluble
In liquid, and continue heating water bath until the paclobutrazol powder and the drop-proof agent powder are dissolved completely in the aqueous solution, ultrasound
5-10min is vibrated, 10-12 hours is stood, obtains culture medium stoste；
In above-mentioned steps b, Vltrasonic device is vibrated according to the relationship of following ultrasonic vibration frequencies and heating temperature,
Wherein, in the first temperature section, in 25-40 DEG C of temperature range:
In formula, f1Indicate the real-time vibration frequency of the first temperature section, T indicates the real time temperature in the first temperature section, T0Indicate reference
Preset temperature value, temperature are 30 DEG C, and m indicates the hotbed chives weight in the mixed liquor being added, and M indicates the lemon in the mixed liquor being added
The quality of skin, c indicate the specific heat of mixed liquor, f0The default vibration frequency for indicating the first temperature section, is 25kHz；
Wherein, in second temperature section, in 40-45 DEG C of temperature range:
In formula, f2Indicate the real-time vibration frequency of second temperature section, T indicates the real time temperature in second temperature section, T10Indicate ginseng
Preset temperature value is examined, thermostat temperature is 40 DEG C, and m indicates the hotbed chives weight in the mixed liquor being added, and M is indicated in the mixed liquor being added
Lemon peel quality, c indicate mixed liquor specific heat, f10The default vibration frequency for indicating second temperature section, is 55kHz；
In above-mentioned steps d, it is 25kHz that room temperature, which is warming up to the vibration frequency in 45 DEG C of temperature ranges,；
When being warming up to 45 DEG C, in 45-55 DEG C of temperature range:
In formula, f3Indicate the real-time vibration frequency of third temperature section, T indicates the real time temperature in third temperature section, T20Indicate ginseng
Preset temperature value is examined, temperature is 45 DEG C, and c indicates the specific heat of mixed liquor, f20The default vibration frequency for indicating the first temperature section is
40kHz；
Wherein, in temperature-fall period, in 25-55 DEG C of temperature range:
In formula, f4Indicate the real-time vibration frequency of the 4th temperature section, T indicates that the real time temperature in the 4th temperature section, c indicate mixing
The specific heat of liquid, m indicate the hotbed chives weight in the mixed liquor being added, and M indicates the gross mass of the lemon peel in the mixed liquor being added, f30
The default vibration frequency for indicating the 4th temperature section is 30kHz；
Step e: the matrix that step d is made is packed into bacterium bottle, and the matrix during bottling in each bacterium bottle will uniformly, surrounding nothing
Gap, Weight control in 1.6-2.0Kg, bacterium it is bottled it is good after, bottleneck and its surrounding are cleaned out, sealing is put into equipment
Constant temperature sterilizing 12-18h at 100 DEG C, the bacterium bottle after sterilizing move into inoculated and cultured room, drop to 25 DEG C or less to bacterium bottle temperature；
Step f: Spawn incubation: gained bacterium bottle in step e is suspended in culturing room, keeping culture room temperature is 20-25 DEG C, empty
Air humidity degree≤75% opens the comprehensive irradiation bacterium bottle 10-20min of ultraviolet light every 3-5h, while being aided with infra-red radiation, until
Mycelia sends out full bottle bacterium, and mycelia is exposed and receives sunlight according to solarization, and discontinuity is sprayed water, until needle mushroom is grown up；
Step g: timely collecting: picking mature needle mushroom, adopted first batch of needle mushroom, and by bacterium bottle, u-turn is put up and down
It puts, and fills the water, continue to pick when needle mushroom grows up to.
2. the method that bottle according to claim 1 plants yellow needle mushroom, which is characterized in that the culture medium stoste include with
The component of lower parts by weight: 10-15 parts of lemon peel, 5-8 parts of sugarcane, 5-10 parts of hotbed chives, 3-6 parts of millet flour, 3-6 parts of pumpkin, chlorination
0.5-1 parts of sodium, 0.3-0.5 parts of yeast powder, 0.5-1 parts of tomato extracting solution, 0.3-0.5 parts of paclobutrazol powder, is prevented 2-3 parts of dried silkworm chrysalis meal
Fall plain powder 0.2-0.4 parts, 3-5 parts of chitosan, 0.5-2.5 parts of magnesium carbonate, 20-25 parts of trehalose, nanometer calcium carbonate 1.3-9.5
Part, 0.2-0.8 parts of sodium lauryl sulfonate.
3. the method that bottle according to claim 2 plants yellow needle mushroom, which is characterized in that the lemon peel is in manufacturing process
In, after choosing Fresh Lemon peeling, each lemon peel sheet weight impregnates 3-4d in 3% sodium chloride solution between 2-3g,
It dries, is pulverized into powder, it is spare.
4. the method that bottle according to claim 3 plants yellow needle mushroom, which is characterized in that in the manufacturing process of the hotbed chives
In, fresh hotbed chives are chosen, the segment that length is 0.5-1cm is cut to, is mixed with above-mentioned powdered lemon peel part, stirring is equal
It is even, add the water of 10 times of weight, at 25-40 DEG C at a temperature of edging up, vibrates, stand.
5. the method that bottle according to claim 2 plants yellow needle mushroom, which is characterized in that in the manufacturing process of sugarcane,
It chooses without insect pest, disease-free, without edge of a knife damage sugarcane, the diameter of every sugarcane is 2-2.5cm, length 2.2-2.5m, clearly
After washing surface, it is cut into the uniform cylindrical shape dissection that size is 2-3cm, after wrapping up with reverse osmosis membrane, in 1% Betagen Solution
It impregnates 2-4 hours；Vitamin A is injected into sugarcane with the method impregnated and syringe is injected respectively during plantation and after harvest
With vitamin E mixed liquor 20-40mL.
6. the method that bottle according to claim 5 plants yellow needle mushroom, which is characterized in that in planting process, exist respectively
Third month after growing cane, the 6th month and progress one injection in nine month, injection point are sugarcane root, and injection volume is
2-4mL；Another kind is the middle position of each sugarcane dissection after sugarcane maturation dissection, is circumferentially uniformly chosen
Four injection points are injected vitamin A and 3 3-4mL of vitamin E respectively, are infused respectively at the both ends cut surface center of billets of cane
0 3mL-15mL of vitamin A and vitamin e1 is penetrated, uniformly applies berley salt 2-3g in cut surface later, and whole with preservative film covering
A billets of cane stands 2-4 hours, is added in pulverizer, sugarcane clast after crushing, the vitamin A and vitamin for being 5% with concentration
E ethyl acetate solution impregnates 3-5 hours, pulls out, dries, processed sugarcane is made.
7. the method that bottle according to claim 6 plants yellow needle mushroom, which is characterized in that described small in above-mentioned steps a
Rice flour, pumpkin, bean cake powder, dried silkworm chrysalis meal, yeast powder granularity be 60-80 mesh.
8. the method that bottle according to claim 2 plants yellow needle mushroom, which is characterized in that during above-mentioned Spawn incubation,
The UV illumination intensity is 2-3.5 μ w/cm2。
9. the method that bottle according to claim 2 plants yellow needle mushroom, which is characterized in that during above-mentioned Spawn incubation,
The yellow water that bacteria stick spues need to be rinsed well when water spray in time, prevent yellow water from condensing in bacteria stick；During above-mentioned Spawn incubation,
Mycelia needs to carry out that ear is urged to handle to mycelia after growing；It urges kept the temperature with plastic film covering bacterium bottle daytime during ear, controls
Its temperature≤28 DEG C is made, night opens plastic film and cools down, and continuous processing 5-7 days.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110122164A (en) * | 2019-05-22 | 2019-08-16 | 山东友和菌业有限公司 | One plant of yellow needle mushroom bacterial strain and its breeding method |
| CN114190224A (en) * | 2021-10-28 | 2022-03-18 | 连云港丰泽源生物科技有限公司 | Method for improving content of lysine in needle mushroom |
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| CN103724077A (en) * | 2013-12-12 | 2014-04-16 | 凤台县千秋食用菌有限公司 | Sorghum-skin-containing needle mushroom cultivation material and preparation method thereof |
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| CN103724077A (en) * | 2013-12-12 | 2014-04-16 | 凤台县千秋食用菌有限公司 | Sorghum-skin-containing needle mushroom cultivation material and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110122164A (en) * | 2019-05-22 | 2019-08-16 | 山东友和菌业有限公司 | One plant of yellow needle mushroom bacterial strain and its breeding method |
| CN114190224A (en) * | 2021-10-28 | 2022-03-18 | 连云港丰泽源生物科技有限公司 | Method for improving content of lysine in needle mushroom |
| CN114190224B (en) * | 2021-10-28 | 2023-12-22 | 连云港丰泽源生物科技有限公司 | Method for improving lysine content of flammulina velutipes |
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Application publication date: 20190329 |