CN109699395A - A kind of bottle of cultivation needle mushroom breeding method - Google Patents
A kind of bottle of cultivation needle mushroom breeding method Download PDFInfo
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- CN109699395A CN109699395A CN201910194646.0A CN201910194646A CN109699395A CN 109699395 A CN109699395 A CN 109699395A CN 201910194646 A CN201910194646 A CN 201910194646A CN 109699395 A CN109699395 A CN 109699395A
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Abstract
The present invention relates to a kind of bottle of cultivation needle mushroom breeding methods, 10-15 parts of the persimmon of preparation, 10-15 parts of preserved vegetable, 5-8 parts of kaoliang stalk, 5-10 parts of epipremnum aureum leaf, 3-6 parts of millet flour, 3-6 parts of corn flour, 0.5-1 parts of sodium chloride, 2-3 parts of locust powder, 0.3-0.5 parts of yeast powder, 0.5-1 parts of shallot, 0.3-0.5 parts of paclobutrazol powder, 0.2-0.4 parts of drop-proof agent powder, 3-5 parts of chitosan, 0.5-2.5 parts of magnesium carbonate, 20-25 parts of sucrose, 1.3-9.5 parts of nanometer calcium carbonate, 0.2-0.8 parts of sodium lauryl sulfonate, it is added in the aqueous solution and dissolves according to the paclobutrazol powder of parts by weight and the drop-proof agent powder of parts by weight, the continuous heating in 75-95 DEG C of reactor tank, pressure tank is reacted in 0.7-1MPa, Humidity is 25-30%, and gas concentration lwevel uses ultraviolet light irradiation 45-50min in 12-15%, stands 10-12 hours, obtain culture medium stoste.
Description
Technical field
The present invention relates to needle mushroom Cultivating techniques field more particularly to a kind of bottle of cultivation needle mushroom breeding methods.
Background technique
Needle mushroom scientific name hair handle money bacterium, also known as hair handle small fire mushroom, plain mushroom, dried mushroom, plain wild rice, freeze bacterium, golden mushroom, intelligence at structure bacterium
Power mushroom etc..Because its stem is elongated, like day lily, therefore claims needle mushroom, belong to Agaricales Tricholomataceae needle gold mushroom category, be a kind of bacterium algae lichens
Class.Needle mushroom has very high medicinal dietary function.
Needle mushroom is both a kind of ticbit and preferable health food, and the domestic and international market of needle mushroom is increasingly wide.
Needle mushroom artificial cultivation technique is simultaneously uncomplicated, as long as can control environmental condition, is easy for obtaining reliable and stable yield.According to survey
Fixed, the content of needle mushroom amino acid is very rich, is higher than general mushroom class, reaches in every 100g fresh mushroom containing total amino acid content
20.9mg, wherein 8 kinds of essential amino acids account for the 42.29~51.17% of total amount, isoleucine and content of glutamic acid highest,
Arginine and lysine content are higher in essential amino acid, and the arginine of high level can prevent and treat hepatitis, stomach and intestine feedback disease
Etc. disease of digestive systems, lysine can promote upgrowth and development of children, enhancing memory improves intelligence.Contain egg in needle mushroom dry product
White matter 8.87%, carbohydrate 60.2%, crude fibre are often edible to prevent and treat canker up to 7.4%.But it is normal using this field
Under the premise of rule culture medium, conventional culture methods are cultivated, the content of above-mentioned nutritional ingredient is difficult to increase again.
Meanwhile needle mushroom has aqueous height, tissue tender and crisp, easily causes to damage in harvesting and transporting procedures, causes to become
Color, it is rotten or rotten the features such as.Needle mushroom post harvest transport major physiological Biochemical changes include Tissue Browning, cell wall protein and
Polysaccharide degradation, fructification aging etc., these variations have seriously affected needle mushroom quality.Shorten the main original of needle mushroom shelf life
Because including enzymatic browning and fungus-caused mildew.Cause three kinds of key enzyme polyphenol oxidase PPO, peroxidase of brown stain
POD, cat catalase are distributed in needle mushroom tissue in compartmentalization: the enzymatic activity of cap is minimum, stem top enzymatic activity slightly
Height, middle part is higher, active lower is most strong.Therefore, in storage, the brown stain of needle mushroom is by stem lower part, gradually to
Vertical spread.In the case where no any fresh-keeping measure, the needle mushroom after cleaning will soon cover with white under preference temperature
Fungal hyphae.
Summary of the invention
The purpose of the present invention is to provide a kind of bottle of cultivation needle mushroom breeding methods, to overcome the technology of the prior art to lack
It falls into.
To achieve the above object, the present invention provides a kind of bottle of cultivation needle mushroom breeding method, comprising:
Step a: after choosing fresh persimmon peeling, each Pericarpium Kaki sheet weight is between 2-3g, in 3% sodium chloride solution
Middle immersion 3-4d, dries, is pulverized into powder, spare；Fresh preserved vegetable is chosen, 3-4d is impregnated in 3% sodium chloride solution, dries in the air
It is dry, it is pulverized into powder, it is spare；
Step b: choosing fresh epipremnum aureum leaf, and being cut to area is 0.5-1cm2Segment, with above-mentioned powdered Pericarpium Kaki
Part mixing, stirs evenly, adds the water of 10 times of weight, at 30-50 DEG C at a temperature of edging up, vibrates, stands；
Step c: it chooses without insect pest, disease-free kaoliang stalk, the diameter of every kaoliang stalk is 2-2.5cm, length 2.2-
2.5m after clean the surface, is cut into the uniform cylindrical shape dissection that size is 2-3cm, after wrapping up with reverse osmosis membrane, in 1% povidone
It is impregnated 2-4 hours in iodine solution；Respectively with the method for immersion and syringe injection into kaoliang stalk during plantation and after harvest
Inject vitamin A and vitamin E mixed liquor 20-40mL；
Step d: by 10-15 parts of the persimmon of above-mentioned preparation, 10-15 parts of preserved vegetable, 5-8 parts of kaoliang stalk, 5-10 parts of epipremnum aureum leaf, small
3-6 parts of rice flour, 3-6 parts of corn flour, 0.5-1 parts of sodium chloride, 2-3 parts of locust powder, 0.3-0.5 parts of yeast powder, 0.5-1 parts of shallot,
0.3-0.5 parts of paclobutrazol powder, 0.2-0.4 parts of drop-proof agent powder, 3-5 parts of chitosan, 0.5-2.5 parts of magnesium carbonate, 20-25 parts of sucrose,
1.3-9.5 parts of nanometer calcium carbonate, 0.2-0.8 parts of sodium lauryl sulfonate, according to parts by weight paclobutrazol powder and parts by weight it is anti-fall
Plain powder, which is added in the aqueous solution, to be dissolved, the continuous heating in 75-95 DEG C of reactor tank, and reaction pressure tank is wet in 0.7-1MPa
Degree is 25-30%, and gas concentration lwevel uses ultraviolet light irradiation 45-50min in 12-15%, stands 10-12 hours, obtain
Culture substrate；
Step e: the matrix that above-mentioned steps are made is packed into bacterium bottle, and the matrix during bottling in each bacterium bottle is equal
It is even, surrounding tight, Weight control in 1.6-2.0Kg, bacterium it is bottled it is good after, bottleneck and its surrounding are cleaned out, sealing is put
Enter in equipment the constant temperature at 100 DEG C to sterilize 12-18h, the bacterium bottle after sterilizing moves into inoculated and cultured room, drops to bacterium bottle temperature
25 DEG C or less；10-15 inoculation hole is beaten on each bacterium bottle, the depth of inoculation hole is 2-3cm, aperture 1.5-2cm, sterile
Under the conditions of will Needle mushroom strain be placed in inoculation hole in；
Step f: gained bacterium bottle in above-mentioned steps is suspended in culturing room, keeping culture room temperature is 20-25 DEG C, air
Humidity≤75% opens the comprehensive irradiation bacterium bottle 10-20min of ultraviolet light every 3-5h, while being aided with infra-red radiation, until bacterium
Silk sends out full bottle bacterium, unloads bacterium bottle be erected on culturing rack later, and opens 10-15 V-type mouth around bacterium bottle with blade, makes bacterium
Silk, which is exposed, receives sunlight according to solarization, and discontinuity is sprayed water, until needle mushroom is grown up；Mature needle mushroom is adopted
It plucks, has adopted first batch of needle mushroom, u-turn is put up and down by bacterium bottle, and is filled the water, and continues to pick when needle mushroom grows up to.
Further, growth mediator agent is additionally added in culture substrate, the growth mediator agent includes: the growth of 20-30%
The paclobutrazol and 4-10% for adjusting phosphine, 14-19% of element, the gibberellin of 18-26%, the kinetin of 10-15%, 8-13%
Thiocarbamide.
Further, in above-mentioned matrix configuration process, the mixture needs to impregnate 10-12h after adding water.
Further, during above-mentioned Spawn incubation, the UV illumination intensity is 2-3.5 μ w/cm2。
Further, during above-mentioned Spawn incubation, the yellow water that bacteria stick spues need to be rinsed well when water spray in time, is prevented
Yellow water condenses in bacteria stick；During above-mentioned Spawn incubation, mycelia needs to carry out that ear is urged to handle to mycelia after growing；Urge ear process
Middle daytime covers bacterium bottle with plastic film and is kept the temperature, and controls its temperature≤28 DEG C, night opens plastic film cooling, continuous to locate
Reason 5-7 days.
Further, the pressure sensor detected to pressure is set on the reaction top tank structure, the reactor tank
It is additionally provided with several circle heater strips on side wall, humidity sensor is set on the side wall of the reactor tank, further includes being arranged in tank
External control panel, the real-time detection information that each sensor will test are transmitted in control panel, and according to testing result with
Default result is controlled, and control panel controls control valve.
Further, in above-mentioned steps d, reactor tank is pressed according to the relationship of following reaction pressure tanks and heating temperature
Power control, wherein in the first temperature section, in 75-80 DEG C of temperature range:
In formula, p1Indicate the real-time pressure value of the first temperature section, T indicates the real time temperature in the first temperature section, T0Indicate ginseng
Preset temperature value is examined, temperature is 75 DEG C, and m indicates the persimmon weight in the mixed liquor being added, and M indicates green in the mixed liquor being added
The quality of Luo Ye, preserved vegetable and persimmon, c indicate the specific heat of mixed liquor, P0The preset pressure value for indicating the first temperature section is
0.7MPa；
Wherein, in second temperature section, in 80-85 DEG C of temperature range:
In formula, P2Indicate the real-time pressure value of second temperature section, T indicates the real time temperature in second temperature section, T10It indicates
With reference to preset temperature value, thermostat temperature is 80 DEG C, and m indicates the persimmon weight in the mixed liquor being added, and M indicates the mixed liquor being added
In epipremnum aureum leaf, preserved vegetable and persimmon quality, c indicate mixed liquor specific heat, P10Indicate the preset pressure value of second temperature section,
For 0.8MPa；
When being warming up to 85 DEG C, in 85-95 DEG C of temperature range:
In formula, P3Indicate the real-time pressure controlling value frequency of third temperature section, T indicates the real-time temperature in third temperature section
Degree, T20It indicates to refer to preset temperature value, temperature is 95 DEG C, and c indicates the specific heat of mixed liquor, P20Indicate the default of the first temperature section
Pressure value is 0.9MPa；
Wherein, in temperature-fall period, in 75-95 DEG C of temperature range:
In formula, P4Indicate the real-time pressure value of the 4th temperature section, T indicates that the real time temperature in the 4th temperature section, c indicate mixed
The specific heat of liquid is closed, m indicates the persimmon weight in the mixed liquor being added, and M indicates epipremnum aureum leaf, preserved vegetable and the persimmon in the mixed liquor being added
The gross mass of son, P30Indicate the preset pressure value of the 4th temperature section, 0.8MPa.
Further, wherein the millet flour, corn flour, bean cake powder, locust powder, the granularity of yeast powder are 60-80
Mesh.
Further, wherein during above-mentioned Spawn incubation, the yellow water that bacteria stick spues need to be rinsed when water spray in time and be done
Only, prevent yellow water from condensing in bacteria stick；
During above-mentioned Spawn incubation, mycelia needs to carry out that ear is urged to handle to mycelia after growing；
It urges kept the temperature with plastic film covering bacterium bottle daytime during ear, controls its temperature≤28 DEG C, night opens modeling
Expect that film cools down, continuous processing 5-7 days.
Compared with prior art the beneficial effects of the present invention are, bottle of the present invention plants needle mushroom breeding method, using persimmon,
Preserved vegetable, epipremnum aureum leaf are as raw material, wherein contain a variety of active ingredients in persimmon, such as vitamin C, a variety of flavonoid glycosides, diterpene
Class, choline, beta carotene, sucrose rich in, glucose, fructose, protein, carrotene, vitamin C, citrulling,
Iodine, calcium, phosphorus, iron, zinc, and be easy to absorb.Preserved vegetable: containing more than the ten kinds of amino acid beneficial to human body, multivitamin and its
Its microelement；And epipremnum aureum leaf cellulose rich in and a variety of aminoacid ingredients.Needle mushroom made of cultivation, in bacterium
Result substantially is cultivated better than general in terms of shank diameter, total amino acid content, polyoses content.
The present invention is by the comprehensively control to humidity and ultraviolet irradiation intensity, and according to material component, such as epipremnum aureum
The raw material such as leaf, persimmon, to adjust the pressure in tank.Tank body is promoted by increasing correction factor with the reduction of humidity
Interior pressure；Make the reduction of correction factor increasing degree with the reduction of humidity and the increase of ultraviolet irradiation intensity.Cause
This, the present invention quantifies each biological parameter, and the intracorporal pressure value of tank is enable to adjust based on multifactor parameter.
In particular, the present invention uses dynamic controlling value mode to the pressure value in tank, it is in the first stage, raised in temperature
Meanwhile quickly increase the intracorporal pressure value of tank, be sufficiently mixed various composition, in particular, persimmon juice under high pressure with it is a variety of
Reacted constituent rapid fusion；In second stage, as temperature increases, pressure inside the tank is gradually risen, and is kept away being lower than in 85 DEG C of temperature
Exempt from solution boils, merge the ingredients such as persimmon sufficiently, which is optimal reaction temperature；In temperature-fall period, pass through temperature
Reduction, the pressure in tank reduces rapidly, and is sufficiently mixed various composition.
In particular, control technique of the invention is made by controlling the control of frequency under progressive lower temperature environments with pressure
The various nutritional ingredients of the two material can be kept, and drop various vitamin mixing.Different phase of the present invention in kaoliang stalk
It is cultivated using sodium chloride, the addition of treated kaoliang stalk, it is ensured that nutritional ingredient needed for plant growth has significant
Effect.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the golden mushroom cultivating system of the embodiment of the present invention；
Fig. 2 is the structural schematic diagram of the reactor tank of the embodiment of the present invention.
Specific embodiment
Hereinafter, the forgoing and additional technical features and advantages are described in more detail.
It refering to fig. 1, is the structural schematic diagram and reactor tank of the golden mushroom cultivating system of the embodiment of the present invention shown in 2
Structural schematic diagram, the present embodiment system include: reactor tank 1, and the control valve 2 of 1 outlet end of reactor tank is arranged in, is connected to reactor tank
Sterilizing unit 3 outside 1, and aerodynamic compressor 4 is provided, the air that compressor 4 exports is sterilized by sterilizing unit 3
Afterwards, then by the control of control valve 2 input in reactor tank 1 that the reaction was continued.It is arranged in the tank mouth position of the reactor tank of the present embodiment
There is a pressure sensor 13 detected to pressure inside the tank, is provided in the upper area of the reactor tank several to illuminate
Ultraviolet lamp group 16 is additionally provided with several circle heater strips 14 on the side wall of the reactor tank, is arranged on the side wall of the reactor tank
Humidity sensor 12 detects the humidity in tank；Gas concentration lwevel detection is additionally provided on the reaction top tank structure to pass
Sensor 15 in real time detects the gas concentration lwevel in tank.It further include the control panel being arranged in outside tank body, each sensor
The real-time detection information that will test is transmitted in control panel, and is controlled according to testing result with default result, control panel
Control valve 2 is controlled, moisture and carbon dioxide in toilet-cleaning jar, or control compressor injects sky into reactor tank
Gas.
The golden mushroom plantation method of the present embodiment includes:
Step a: after choosing fresh persimmon peeling, each Pericarpium Kaki sheet weight is between 2-3g, in 3% sodium chloride solution
Middle immersion 3-4d, dries, is pulverized into powder, spare；Fresh preserved vegetable is chosen, 3-4d is impregnated in 3% sodium chloride solution, dries in the air
It is dry, it is pulverized into powder, it is spare；
Step b: choosing fresh epipremnum aureum leaf, and being cut to area is 0.5-1cm2Segment, with above-mentioned powdered Pericarpium Kaki
Part mixing, stirs evenly, adds the water of 10 times of weight, at 30-50 DEG C at a temperature of edging up, vibrates, stands；
Step c: it chooses without insect pest, disease-free kaoliang stalk, the diameter of every kaoliang stalk is 2-2.5cm, length 2.2-
2.5m after clean the surface, is cut into the uniform cylindrical shape dissection that size is 2-3cm, after wrapping up with reverse osmosis membrane, in 1% povidone
It is impregnated 2-4 hours in iodine solution；Respectively with the method for immersion and syringe injection into kaoliang stalk during plantation and after harvest
Inject vitamin A and vitamin E mixed liquor 20-40mL；Specifically, the third after planting kaoliang stalk month, the 6th respectively
Progress one injection in a month and nine month, injection point are kaoliang stalk root, injection volume 2-4mL；Another kind is in kaoliang stalk
After mature dissection, the middle position of each kaoliang stalk dissection is circumferentially uniformly chosen four injection points, is injected respectively
3 3-4mL of vitamin A and vitamin E injects vitamin A and vitamin at the both ends cut surface center of sorghum rod segment respectively
E10 3mL-15mL uniformly applies berley salt 2-3g in cut surface later, and covers entire sorghum rod segment with preservative film, stands 2-
It 4 hours, is added in pulverizer, kaoliang stalk clast after crushing, the vitamin A and vitamin E ethyl acetate solution for being 5% with concentration
It impregnates 3-5 hours, pulls out, dry, processed kaoliang stalk is made.
Step d: by 10-15 parts of the persimmon of above-mentioned preparation, 10-15 parts of preserved vegetable, 5-8 parts of kaoliang stalk, 5-10 parts of epipremnum aureum leaf, small
3-6 parts of rice flour, 3-6 parts of corn flour, 0.5-1 parts of sodium chloride, 2-3 parts of locust powder, 0.3-0.5 parts of yeast powder, 0.5-1 parts of shallot,
0.3-0.5 parts of paclobutrazol powder, 0.2-0.4 parts of drop-proof agent powder, 3-5 parts of chitosan, 0.5-2.5 parts of magnesium carbonate, 20-25 parts of sucrose,
1.3-9.5 parts of nanometer calcium carbonate, 0.2-0.8 parts of sodium lauryl sulfonate, according to parts by weight paclobutrazol powder and parts by weight it is anti-fall
Plain powder, which is added in the aqueous solution, to be dissolved, the continuous heating in 75-95 DEG C of reactor tank, and reaction pressure tank is wet in 0.7-1MPa
Degree is 25-30%, and gas concentration lwevel uses ultraviolet light irradiation 45-50min in 12-15%, stands 10-12 hours, obtain
Culture substrate；
Step e: the matrix that above-mentioned steps are made is packed into bacterium bottle, and the matrix during bottling in each bacterium bottle is equal
It is even, surrounding tight, Weight control in 1.6-2.0Kg, bacterium it is bottled it is good after, bottleneck and its surrounding are cleaned out, sealing is put
Enter in equipment the constant temperature at 100 DEG C to sterilize 12-18h, the bacterium bottle after sterilizing moves into inoculated and cultured room, drops to bacterium bottle temperature
25 DEG C or less；10-15 inoculation hole is beaten on each bacterium bottle, the depth of inoculation hole is 2-3cm, aperture 1.5-2cm, sterile
Under the conditions of will Needle mushroom strain be placed in inoculation hole in；
Step f: gained bacterium bottle in above-mentioned steps is suspended in culturing room, keeping culture room temperature is 20-25 DEG C, air
Humidity≤75% opens the comprehensive irradiation bacterium bottle 10-20min of ultraviolet light every 3-5h, while being aided with infra-red radiation, until bacterium
Silk sends out full bottle bacterium, unloads bacterium bottle be erected on culturing rack later, and opens 10-15 V-type mouth around bacterium bottle with blade, makes bacterium
Silk, which is exposed, receives sunlight according to solarization, and discontinuity is sprayed water, until needle mushroom is grown up；Mature needle mushroom is adopted
It plucks, has adopted first batch of needle mushroom, u-turn is put up and down by bacterium bottle, and is filled the water, and continues to pick when needle mushroom grows up to.
Growth mediator agent is additionally added in culture substrate, the growth mediator agent includes: the auxin of 20-30%, 18-26%
Gibberellin, the kinetin of 10-15%, 8-13% adjusting phosphine, the paclobutrazol of 14-19% and the thiocarbamide of 4-10%.
In above-mentioned matrix configuration process, the mixture needs to impregnate 10-12h after adding water.
During above-mentioned Spawn incubation, the UV illumination intensity is 2-3.5 μ w/cm2。
During above-mentioned Spawn incubation, the yellow water that bacteria stick spues need to be rinsed well when water spray in time, prevent yellow water in bacterium
It is condensed on stick；During above-mentioned Spawn incubation, mycelia needs to carry out that ear is urged to handle to mycelia after growing；Urge use daytime during ear
Plastic film covering bacterium bottle is kept the temperature, its temperature≤28 DEG C is controlled, and night opens plastic film and cools down, and continuous processing 5-7 days.
The pressure sensor detected to pressure is set on the reaction top tank structure, is also set on the side wall of the reactor tank
Several circle heater strips are equipped with, humidity sensor is set on the side wall of the reactor tank, further include the control being arranged in outside tank body
Plate, the real-time detection information that each sensor will test are transmitted in control panel, and according to testing result with default result into
Row control, control panel control control valve.
In above-mentioned steps d, reactor tank carries out pressure control according to the relationship of following reaction pressure tanks and heating temperature,
In, in the first temperature section, in 75-80 DEG C of temperature range:
In formula, p1Indicate the real-time pressure value of the first temperature section, T indicates the real time temperature in the first temperature section, T0Indicate ginseng
Preset temperature value is examined, temperature is 75 DEG C, and m indicates the persimmon weight in the mixed liquor being added, and M indicates green in the mixed liquor being added
The quality of Luo Ye, preserved vegetable and persimmon, c indicate the specific heat of mixed liquor, P0The preset pressure value for indicating the first temperature section is
0.7MPa；
Wherein, in second temperature section, in 80-85 DEG C of temperature range:
In formula, P2Indicate the real-time pressure value of second temperature section, T indicates the real time temperature in second temperature section, T10It indicates
With reference to preset temperature value, thermostat temperature is 80 DEG C, and m indicates the persimmon weight in the mixed liquor being added, and M indicates the mixed liquor being added
In epipremnum aureum leaf, preserved vegetable and persimmon quality, c indicate mixed liquor specific heat, P10Indicate the preset pressure value of second temperature section,
For 0.8MPa；
When being warming up to 85 DEG C, in 85-95 DEG C of temperature range:
In formula, P3Indicate the real-time pressure controlling value frequency of third temperature section, T indicates the real-time temperature in third temperature section
Degree, T20It indicates to refer to preset temperature value, temperature is 95 DEG C, and c indicates the specific heat of mixed liquor, P20Indicate the default of the first temperature section
Pressure value is 0.9MPa；
Wherein, in temperature-fall period, in 75-95 DEG C of temperature range:
In formula, P4Indicate the real-time pressure value of the 4th temperature section, T indicates that the real time temperature in the 4th temperature section, c indicate mixed
The specific heat of liquid is closed, m indicates the persimmon weight in the mixed liquor being added, and M indicates epipremnum aureum leaf, preserved vegetable and the persimmon in the mixed liquor being added
The gross mass of son, P30Indicate the preset pressure value of the 4th temperature section, 0.8MPa.
Wherein, the millet flour, corn flour, bean cake powder, locust powder, the granularity of yeast powder are 60-80 mesh.
Wherein, during above-mentioned Spawn incubation, the yellow water that bacteria stick spues need to be rinsed well when water spray in time, prevents yellow water
It is condensed in bacteria stick；
During above-mentioned Spawn incubation, mycelia needs to carry out that ear is urged to handle to mycelia after growing；
It urges kept the temperature with plastic film covering bacterium bottle daytime during ear, controls its temperature≤28 DEG C, night opens modeling
Expect that film cools down, continuous processing 5-7 days.
Embodiment 1
The present embodiment is cultivated according to above-mentioned cultivating process, is recorded according to following table 1 items.
10 parts of persimmon, 10 parts of preserved vegetable, 5 parts of kaoliang stalk, 5 parts of epipremnum aureum leaf, 3 parts of millet flour, 3 parts of corn flour, sodium chloride 0.5
Part, 2 parts of locust powder, 0.3 part of yeast powder, 0.5 part of shallot, 0.3 part of paclobutrazol powder, 0.2 part of drop-proof agent powder, 3 parts of chitosan, carbonic acid
0.5 part of magnesium, 20 parts of sucrose, 1.3 parts of nanometer calcium carbonate, 0.2 part of sodium lauryl sulfonate.
Embodiment 2
15 parts of persimmon, 15 parts of preserved vegetable, 8 parts of kaoliang stalk, 10 parts of epipremnum aureum leaf, 6 parts of millet flour, 6 parts of corn flour, 1 part of sodium chloride,
3 parts of locust powder, 0.5 part of yeast powder, 1 part of shallot, 0.5 part of paclobutrazol powder, 0.4 part of drop-proof agent powder, 5 parts of chitosan, magnesium carbonate 2.5
Part, 25 parts of sucrose, 9.5 parts of nanometer calcium carbonate, 0.8 part of sodium lauryl sulfonate.
Embodiment 3
12 parts of persimmon, 12 parts of preserved vegetable, 6 parts of kaoliang stalk, 6 parts of epipremnum aureum leaf, 4 parts of millet flour, 4 parts of corn flour, sodium chloride 0.6
Part, 2.2 parts of locust powder, 0.4 part of yeast powder, 0.7 part of shallot, 0.4 part of paclobutrazol powder, 0.3 part of drop-proof agent powder, 4 parts of chitosan, carbon
1 part of sour magnesium, 22 parts of sucrose, 4 parts of nanometer calcium carbonate, 0.4 part of sodium lauryl sulfonate.
Embodiment 4
14 parts of persimmon, 14 parts of preserved vegetable, 7 parts of kaoliang stalk, 8 parts of epipremnum aureum leaf, 5 parts of millet flour, 5 parts of corn flour, sodium chloride 0.9
Part, 2.8 parts of locust powder, 0.45 part of yeast powder, 0.8 part of shallot, 0.45 part of paclobutrazol powder, 0.35 part of drop-proof agent powder, chitosan 4.5
Part, 2.2 parts of magnesium carbonate, 23 parts of sucrose, 7 parts of nanometer calcium carbonate, 0.7 part of sodium lauryl sulfonate.
Control group 1
Culture medium comprises the following components in parts by weight: 5 parts of millet flour, 5 parts of corn flour, 0.8 part of sodium chloride, locust powder 2.5
Part, 0.4 part of yeast powder, 0.8 part of shallot, 0.4 part of paclobutrazol powder, 0.3 part of drop-proof agent powder, 3.5 parts of chitosan, 2 parts of magnesium carbonate, sugarcane
22 parts, 6 parts of nanometer calcium carbonate, 0.5 part of sodium lauryl sulfonate of sugar.
Unlike above-described embodiment 1, in above-mentioned steps d, reactor tank is according to holding constant pressure 0.8MPa.
Control group 2
Culture medium comprises the following components in parts by weight: 5 parts of millet flour, 5 parts of corn flour, 0.8 part of sodium chloride, locust powder 2.5
Part, 0.4 part of yeast powder, 0.8 part of shallot, 0.4 part of paclobutrazol powder, 0.3 part of drop-proof agent powder, 3.5 parts of chitosan, 2 parts of magnesium carbonate, sugarcane
22 parts, 6 parts of nanometer calcium carbonate, 0.5 part of sodium lauryl sulfonate of sugar.
It is as follows for the growing state result mean value for cultivating mould measurement and needle mushroom of above each embodiment:
So far, preferred embodiment shown in having been combined describes technical solution of the present invention, still, art technology
Personnel are it is easily understood that protection scope of the present invention is expressly not limited to these specific embodiments.Without departing from the present invention
Principle under the premise of, those skilled in the art can make equivalent change or replacement to the relevant technologies feature, these change
Or the technical solution after replacement will fall within the scope of protection of the present invention.
Claims (9)
1. a kind of bottle of cultivation needle mushroom breeding method characterized by comprising
Step a: after choosing fresh persimmon peeling, each Pericarpium Kaki sheet weight is soaked in 3% sodium chloride solution between 2-3g
3-4d is steeped, dries, is pulverized into powder, it is spare；Fresh preserved vegetable is chosen, 3-4d is impregnated in 3% sodium chloride solution, dries,
It is pulverized into powder, it is spare；
Step b: choosing fresh epipremnum aureum leaf, and being cut to area is 0.5-1cm2Segment, it is mixed with above-mentioned powdered Pericarpium Kaki part
It closes, stirs evenly, add the water of 10 times of weight, at 30-50 DEG C at a temperature of edging up, vibrate, stand；
Step c: choosing without insect pest, disease-free kaoliang stalk, and the diameter of every kaoliang stalk is 2-2.5cm, length 2.2-2.5m,
After clean the surface, it is cut into the uniform cylindrical shape dissection that size is 2-3cm, after wrapping up with reverse osmosis membrane, in 1% Betagen Solution
Middle immersion 2-4 hours；Dimension is injected into kaoliang stalk with the method for immersion and syringe injection respectively during plantation and after harvest
Raw element A and vitamin E mixed liquor 20-40mL；
Step d: by 10-15 parts of the persimmon of above-mentioned preparation, 10-15 parts of preserved vegetable, 5-8 parts of kaoliang stalk, 5-10 parts of epipremnum aureum leaf, millet flour
3-6 parts, 3-6 parts of corn flour, 0.5-1 parts of sodium chloride, 2-3 parts of locust powder, 0.3-0.5 parts of yeast powder, 0.5-1 parts of shallot, multiple-effect
0.3-0.5 parts of azoles powder, 0.2-0.4 parts of drop-proof agent powder, 3-5 parts of chitosan, 0.5-2.5 parts of magnesium carbonate, 20-25 parts of sucrose, nanometer
1.3-9.5 parts of calcium carbonate, 0.2-0.8 parts of sodium lauryl sulfonate, according to the paclobutrazol powder of parts by weight and the drop-proof agent powder of parts by weight
It is added in the aqueous solution and dissolves, the continuous heating in 75-95 DEG C of reactor tank reacts pressure tank in 0.7-1MPa, and humidity is
25-30%, gas concentration lwevel use ultraviolet light irradiation 45-50mi n in 12-15%, stand 10-12 hours, trained
Support matrix；
Step e: the matrix that above-mentioned steps are made is packed into bacterium bottle, and the matrix during bottling in each bacterium bottle will uniformly, week
Enclose tight, Weight control in 1.6-2.0Kg, bacterium it is bottled it is good after, bottleneck and its surrounding are cleaned out, sealing is put into equipment
In at 100 DEG C constant temperature sterilize 12-18h, after sterilizing bacterium bottle move into inoculated and cultured room in, to bacterium bottle temperature drop to 25 DEG C with
Under；Beat 10-15 inoculation hole on each bacterium bottle, the depth of inoculation hole is 2-3cm, aperture 1.5-2cm, aseptically
Needle mushroom strain is placed in inoculation hole；
Step f: gained bacterium bottle in above-mentioned steps is suspended in culturing room, keeping culture room temperature is 20-25 DEG C, air humidity
≤ 75%, the comprehensive irradiation bacterium bottle 10-20mi n of ultraviolet light is opened every 3-5h, while being aided with infra-red radiation, until mycelia
Full bottle bacterium is sent out, bacterium bottle is unloaded be erected on culturing rack later, and opens 10-15 V-type mouth around bacterium bottle with blade, makes mycelia
It is exposed and receives sunlight according to solarization, and discontinuity is sprayed water, until needle mushroom is grown up；Mature needle mushroom is picked,
First batch of needle mushroom is adopted, u-turn is put up and down by bacterium bottle, and is filled the water, and continues to pick when needle mushroom grows up to.
2. bottle according to claim 1 plants needle mushroom breeding method, which is characterized in that be additionally added growth in culture substrate and adjust
Agent is solved, the growth mediator agent includes: the auxin of 20-30%, the gibberellin of 18-26%, the kinetin of 10-15%, 8-
13% adjusting phosphine, the paclobutrazol of 14-19% and the thiocarbamide of 4-10%.
3. bottle according to claim 1 plants needle mushroom breeding method, which is characterized in that in above-mentioned matrix configuration process, institute
Stating after mixture adds water needs to impregnate 10-12h.
4. bottle according to claim 2 plants needle mushroom breeding method, which is characterized in that during above-mentioned Spawn incubation, institute
Stating UV illumination intensity is 2-3.5 μ w/cm2。
5. bottle according to claim 2 plants needle mushroom breeding method, which is characterized in that during above-mentioned Spawn incubation, spray
The yellow water that bacteria stick spues need to be rinsed well when water in time, prevent yellow water from condensing in bacteria stick；During above-mentioned Spawn incubation, bacterium
Filament length needs to carry out that ear is urged to handle to mycelia after going out；It urges kept the temperature with plastic film covering bacterium bottle daytime during ear, controls
Its temperature≤28 DEG C, night open plastic film and cool down, and continuous processing 5-7 days.
6. bottle according to claim 2 plants needle mushroom breeding method, which is characterized in that setting pair on the reaction top tank structure
The pressure sensor that pressure is detected is additionally provided with several circle heater strips on the side wall of the reactor tank, in the reactor tank
Side wall on humidity sensor is set, further include the control panel being arranged in outside tank body, the real-time inspection that each sensor will test
Measurement information is transmitted in control panel, and is controlled according to testing result with default result, and control panel controls control valve.
7. bottle according to claim 6 plants needle mushroom breeding method, which is characterized in that in above-mentioned steps d, reactor tank is pressed
Pressure control is carried out according to the relationship of following reaction pressure tanks and heating temperature, wherein in the first temperature section, in 75-80 DEG C of temperature
In range:
In formula, p1Indicate the real-time pressure value of the first temperature section, T indicates the real time temperature in the first temperature section, T0It indicates with reference to pre-
If temperature value, temperature is 75 DEG C, and m indicates the persimmon weight in the mixed liquor being added, and M indicates the epipremnum aureum in the mixed liquor being added
The quality of leaf, preserved vegetable and persimmon, c indicate the specific heat of mixed liquor, P0The preset pressure value for indicating the first temperature section is
0.7MPa；
Wherein, in second temperature section, in 80-85 DEG C of temperature range:
In formula, P2Indicate the real-time pressure value of second temperature section, T indicates the real time temperature in second temperature section, T10Indicate reference
Preset temperature value, thermostat temperature are 80 DEG C, and m indicates the persimmon weight in the mixed liquor being added, and M is indicated in the mixed liquor being added
The quality of epipremnum aureum leaf, preserved vegetable and persimmon, c indicate the specific heat of mixed liquor, P10Indicate second temperature section preset pressure value, be
0.8MPa；
When being warming up to 85 DEG C, in 85-95 DEG C of temperature range:
In formula, P3Indicate the real-time pressure controlling value frequency of third temperature section, T indicates the real time temperature in third temperature section, T20
It indicates to refer to preset temperature value, temperature is 95 DEG C, and c indicates the specific heat of mixed liquor, P20Indicate the preset pressure value of the first temperature section,
It is 0.9MPa；
Wherein, in temperature-fall period, in 75-95 DEG C of temperature range:
In formula, P4Indicate the real-time pressure value of the 4th temperature section, T indicates that the real time temperature in the 4th temperature section, c indicate mixed liquor
Specific heat, m indicates the persimmon weight in the mixed liquor being added, and M indicates epipremnum aureum leaf in the mixed liquor being added, preserved vegetable and persimmon
Gross mass, P30Indicate the preset pressure value of the 4th temperature section, 0.8MPa.
8. bottle according to claim 7 plants needle mushroom breeding method, which is characterized in that wherein, the millet flour, corn
Powder, bean cake powder, locust powder, the granularity of yeast powder are 60-80 mesh.
9. bottle according to claim 8 plants needle mushroom breeding method, which is characterized in that wherein, above-mentioned Spawn incubation process
In, the yellow water that bacteria stick spues need to be rinsed well when water spray in time, prevent yellow water from condensing in bacteria stick；
During above-mentioned Spawn incubation, mycelia needs to carry out that ear is urged to handle to mycelia after growing；
It urges kept the temperature with plastic film covering bacterium bottle daytime during ear, controls its temperature≤28 DEG C, it is thin that night opens plastics
Film cooling, continuous processing 5-7 days.
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| CN112166948A (en) * | 2019-07-03 | 2021-01-05 | 南京金万辰生物科技有限公司 | Pollution control method for needle mushroom in germination stage |
| CN112997805A (en) * | 2021-03-31 | 2021-06-22 | 广东雄达实业发展有限公司 | Agrocybe cylindracea cultivation method taking stem raw material of camellia fruit tree as culture medium |
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