CN109548963A - A kind of mixed type the feed for piglet additive and preparation method thereof - Google Patents
A kind of mixed type the feed for piglet additive and preparation method thereof Download PDFInfo
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- CN109548963A CN109548963A CN201910000693.7A CN201910000693A CN109548963A CN 109548963 A CN109548963 A CN 109548963A CN 201910000693 A CN201910000693 A CN 201910000693A CN 109548963 A CN109548963 A CN 109548963A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
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Abstract
The invention belongs to technical field of animal, a kind of mixed type the feed for piglet additive and preparation method thereof is disclosed；The mixed type the feed for piglet additive is made of microorganism and xylose alcoholase；Microorganism includes bacillus subtilis >=8.5 × 109Cfu/g, clostridium butyricum >=5.0 × 108Cfu/g, saccharomyces cerevisiae >=1.0 × 109cfu/g；Xylitol enzyme activity is >=103U/g.The preparation method of the mixed type the feed for piglet additive: carrying out powder processed for bacillus subtilis, clostridium butyricum, saccharomyces cerevisiae and xylose alcoholase, is uniformly mixed, and obtains mixed type the feed for piglet additive；Determination of moisture is carried out to mixed type the feed for piglet additive；Health detection；Test to mixed type the feed for piglet additive: exfactory inspection, type inspection are reviewed；Label, packaging, transport and storage are carried out to mixed type the feed for piglet additive.
Description
Technical field
The invention belongs to technical field of animal more particularly to a kind of mixed type the feed for piglet additive and its preparations
Method.
Background technique
Currently, the prior art commonly used in the trade is such that suckling piglet is the last fortnight (weight 25kg) after coming into being to wean
Pig claims suckling piglet.Suckling piglet is the period of entire pig growth phase most critical, and the basic phase grown up, this stage characteristic are
The histoorgans relative growth rate such as muscle, bone is fast, and physiological function is not perfect, and gastric acid secretion is insufficient, protease, amylase etc.
Digestive ferment hyposecretion reduces feed digestibility, easily causes indigestion and diarrhea occurs.
In conclusion problem of the existing technology is:
(1) medicated premix is excessively used, thus brings food-safety problem.
(2) feed digestibility is low, diarrhea rate is high.
(3) it is badly in need of the feed addictive of safety, noresidue, improves feed digestibility, diarrhea rate is effectively reduced.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of mixed type the feed for piglet additive and its preparations
Method,
The invention is realized in this way a kind of mixed type the feed for piglet additive is made of microorganism and xylose alcoholase.
Further, microorganism includes bacillus subtilis >=8.5 × 109Cfu/g (is had by Beijing good friend biotechnology of touring the heavens
Responsible company is limited to provide), clostridium butyricum >=5.0 × 108Cfu/g (offer of the Zhejiang biotech inc Hui Jia) makes
Brewer yeast >=1.0 × 109Cfu/g (offer of Hubei Angel biology Group Co., Ltd)；
Further, function bacterium sum >=10.0 × 109cfu/g；
Further, xylitol enzyme activity is >=103U/g (offer of Hunan Bioisystech Co., Ltd, You Lianhe nation).
Another object of the present invention is to provide the preparation methods of mixed type the feed for piglet additive:
Step 1: carrying out powder processed for bacillus subtilis, clostridium butyricum, saccharomyces cerevisiae and xylose alcoholase, is uniformly mixed, obtains
Obtain mixed type the feed for piglet additive；
Step 2: determination of moisture is carried out to mixed type the feed for piglet additive；Health detection；
Step 3: test to mixed type the feed for piglet additive: exfactory inspection, type inspection are reviewed；
Step 4: label, packaging, transport and storage are carried out to mixed type the feed for piglet additive.
Further, in step 1, powder obtained, uniform color is consistent, no to agglomerate, is different without moldy metamorphism and peculiar smell
It smells.
Further, in step 1, product grading is all to analyze to sieve by 1.25mm, and 0.80mm analysis sieve oversize is little
In 10%.
Further, in step 1, mixing should uniformly, and the coefficient of variation (CV) of the uniformity is not more than 7% after tested.
Further, in step 2, moisture is not more than 10.0%.
Further, in step 2, sanitary index, total arsenic≤2mg/kg, lead≤8mg/kg, Salmonella must not be detected,
Total number of molds < 4.0 × 104cfu/g。
Further, in step 3, exfactory inspection: factory product is responsible for inspection by our company's quality testing department；Inspection project is
Sense organ, granularity, moisture, function bacterium sum, small peptide content；It can dispatch from the factory after the assay was approved.
Further, in step 3, type inspection is all events inspection, every year must not be less than primary；If any following situations it
For the moment, Ying Jinhang type inspection:
When larger change occurs for a) main former, auxiliary material, formula, production technology or equipment；
B) when halt production more than half a year is resumed production again；
C) when exfactory inspection result and last time type inspection result have larger difference；
D) when national departments concerned is spot-check.
Further, in step 3, to the decision rule of the inspection of mixed type the feed for piglet additive are as follows:
The allowable error that testing result determines is executed by GB/T 18823.
Further, in step 3, to the specific reinspection rule of mixed type the feed for piglet additive are as follows:
When product does not meet this standard if any an index in inspection, Ying Chongxin doubles sampling and is rechecked.Reinspection knot
When still having an index not meet this standard in fruit, then this batch of product is judged to rejected product.
Further, in step 4, the standard of the label of mixed type the feed for piglet additive is specifically held by GB10648
Row.
Further, in step 4, to the packaging of mixed type the feed for piglet additive specifically:
Packing material hygienic requirements should meet in GB/T 16764-2006 7.1 requirement, and material should meet GB/T23181-
8.2 regulation in 2008.
Further, the transport in step 4, to mixed type the feed for piglet additive specifically:
Product should prevent from exposing to the sun and rain during transportation, packages in damaged condition, forbid and the mixed fortune of poisonous and harmful substances.
Further, the storage in step 4, to mixed type the feed for piglet additive specifically:
At product should be stored in cool place, divulge information, be dry, forbid to mix storage with poisonous and harmful substances, shelf life of products is 12
Month.
In conclusion advantages of the present invention and good effect are as follows: mixed type the feed for piglet provided in an embodiment of the present invention
After additive client uses, medicated premix is no longer added, can accomplish and add in the past the effect as medicated premix,
It is nonreactive, safety, needs food safety significant compatriots.
Pig reacts the production of different additive
1. the antibiotic in above table is the medicated feed additive that country allows to add in feed.
2. the related data of antibiotic select from R.Brande 1953.
3. drug residue data source is in the provisions of the relevant regulations issued by the State.
By largely testing use, obtain following result (not adding any medicated premix in feed): 1. improve feeding
Expect that 10% or more digestibility, the price of deed improve 5% or more, diarrhea rate controls within 3%；2. can be complete in the feed for piglet
Full alternatives to medication additive, carries out nonreactive cultivation, avoids or reduces livestock products antibiotic residue, and mitigating pollution pressure has
Important realistic meaning.
Detailed description of the invention
Fig. 1 is the flow chart of the preparation method of mixed type the feed for piglet additive provided in an embodiment of the present invention.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Mixed type the feed for piglet additive provided in an embodiment of the present invention is made of microorganism and xylose alcoholase.
Microorganism provided in an embodiment of the present invention includes function bacterium sum >=10.0 × 109Cfu/g, bacillus subtilis >=
8.5×109Cfu/g, clostridium butyricum >=5.0 × 108Cfu/g, saccharomyces cerevisiae >=1.0 × 109cfu/g.Zhejiang Hui Jia biotechnology
Limited liability company buys bacillus subtilis and clostridium butyricum, and Angel Yeast Co., Ltd buys saccharomyces cerevisiae.
Function bacterium sum >=10.0 × 10 provided in an embodiment of the present invention9cfu/g。
Xylitol enzyme activity provided in an embodiment of the present invention is >=103U/g。
Preparation method of the invention is described in detail with reference to the accompanying drawing；
As shown in Figure 1, the preparation method of mixed type the feed for piglet additive provided in an embodiment of the present invention:
S101: carrying out powder processed for bacillus subtilis, clostridium butyricum, saccharomyces cerevisiae and xylose alcoholase, is uniformly mixed, and obtains
Mixed type the feed for piglet additive；
S102: determination of moisture is carried out to mixed type the feed for piglet additive；Health detection；
S103: test to mixed type the feed for piglet additive: exfactory inspection, type inspection are reviewed；
S104: label, packaging, transport and storage are carried out to mixed type the feed for piglet additive.
In step S101, powder obtained provided in an embodiment of the present invention, uniform color is consistent, no to agglomerate, without mouldy
It is rotten and peculiar smell is different smells.
In step S101, product grading provided in an embodiment of the present invention is all to analyze to sieve by 1.25mm, 0.80mm points
Analysis sieve oversize is not more than 10%.
In step S101, mixing provided in an embodiment of the present invention should uniformly, and the coefficient of variation (CV) of the uniformity is no after tested
Greater than 7%.
In step S102, moisture provided in an embodiment of the present invention is not more than 10.0%.
In step S102, sanitary index provided in an embodiment of the present invention, total arsenic≤2mg/kg, lead≤8mg/kg, Salmonella
Bacillus must not detect, total number of molds < 4.0 × 104cfu/g.
In step S103, exfactory inspection provided in an embodiment of the present invention: factory product is responsible for inspection by our company's quality testing department
It tests；Inspection project is sense organ, granularity, moisture, function bacterium sum, small peptide content；It can dispatch from the factory after the assay was approved.
In step S103, type inspection provided in an embodiment of the present invention is all events inspection, every year must not be less than primary；Such as
There are following situations for the moment, Ying Jinhang type inspection:
When larger change occurs for a) main former, auxiliary material, formula, production technology or equipment；
B) when halt production more than half a year is resumed production again；
C) when exfactory inspection result and last time type inspection result have larger difference；
D) when national departments concerned is spot-check.
In step S103, the decision rule of the inspection provided in an embodiment of the present invention to mixed type the feed for piglet additive
Are as follows:
The allowable error that testing result determines is executed by GB/T 18823.
In step S103, the specific reinspection rule provided in an embodiment of the present invention to mixed type the feed for piglet additive
Are as follows:
When product does not meet this standard if any an index in inspection, Ying Chongxin doubles sampling and is rechecked.Reinspection knot
When still having an index not meet this standard in fruit, then this batch of product is judged to rejected product.
In step S104, the standard of the label provided in an embodiment of the present invention to mixed type the feed for piglet additive is specific
It is executed by GB 10648.
In step S104, the packaging provided in an embodiment of the present invention to mixed type the feed for piglet additive specifically:
Packing material hygienic requirements should meet in GB/T 16764-2006 7.1 requirement, and material should meet GB/T 23181-
8.2 regulation in 2008.
In step S104, the transport provided in an embodiment of the present invention to mixed type the feed for piglet additive specifically:
Product should prevent from exposing to the sun and rain during transportation, packages in damaged condition, forbid and the mixed fortune of poisonous and harmful substances.
In step S104, the storage provided in an embodiment of the present invention to mixed type the feed for piglet additive specifically:
At product should be stored in cool place, divulge information, be dry, forbid to mix storage with poisonous and harmful substances, shelf life of products is 12
Month.
Application principle of the invention is described in further detail combined with specific embodiments below；
Embodiment 1；Bacillus subtilis
1 sample preparation, initial suspension and ten times of dilutions
The viable count of Bacillus (bacillus licheniformis and bacillus subtilis) detects
Sample 25g (ml) is weighed with sterile working, is added to containing 0.85% sterile saline of 225mL and with glass
In the triangular flask of glass pearl, homogeneous 30min is vibrated, the initial suspension of 1:10 is made.The initial suspension 1mL for drawing 1:10, adds
Enter 9L0.85% sterile saline, the dilution of 1:100 is made after mixing well.According to sample bacteria containing amount, do further
Ten times of series be incremented by dilution.
2 inoculations and culture
3 suitable dilutions are selected, draw 0.1mL dilution respectively with Sterile pipette, it is flat to be inoculated into nutrient agar
On plate, do twice in parallel, using spreading rod, carefully quickly for coating inoculation liquid in agar surface ware, spreading rod must not contact plate
Edge.One sterile spreading rod of each plate.Coated plate covers, and sets and places 15min in room temperature, keeps inoculum complete
It is absorbed by agar.It overturns above-mentioned plate and sets in 37 ± 1 DEG C of incubators and cultivate 18-24h.
The calculation method and report of bacillus number in 3 samples
A) bacterium colony counts
1) clump count is taken to be counted in 30-300 dilution plates
2) when only one dilution, when average colony number is between 30-300, then with the average colony number multiplied by it
Extension rate (is shown in Table 1).
If 3) there are two dilutions, when average colony number is between 30-300, should come by the ratio of the two total plate count
It determines.If its ratio should count the average of the two less than 2, is counted if more than 2 and wherein dilute lesser total plate count.
4) if the average colony number of three dilutions is greater than 300, should be by the highest average colony number of dilution multiplied by dilute
Release multiple.
It, should be by the minimum average colony number of dilution multiplied by dilution if 5) average colony number of three dilutions is less than 30
Multiple (is shown in Table 1 5).
6) if the average colony number of three dilutions is multiplied not in 30-300 with the average colony number closest to 300 or 30
With extension rate (being shown in Table 1 6).
B) calculation formula calculates the bacterium colony average of two plates of same dilution, (is determined according to colony characteristics, is calculated
Out in sample corresponding strain viable count), according to formula (1) calculate:
Viable count (cfu/g)=target bacterium colony average × extension rate ... ... (1)
Table 1: the example of total plate count method is calculated
Embodiment 2；Clostridium butyricum
The measurement of 1 viable count
1.1 reagents and solution
A) dilution (normal saline dilution liquid): 8.5g sodium chloride is accurately weighed in l000ml volumetric flask, uses distilled water
Constant volume, sterilizing.
B) RCM culture medium:
Peptone 10g, powdered beef 10g, yeast powder 3g, glucose 5g, soluble starch 1g, sodium chloride 5g, sodium acetate 3g,
L-cysteine hydrochloride 0.5g, agar 8-10g, distilled water 1000mL, final pH 6.8 ± 0.1 (25 DEG C), 121 DEG C of sterilizings
30min。
1.2 instrument and equipment
This test uses common laboratory instrument: super workbench, shaking table: rotary 200r/min, constant incubator, going out
Bacterium pot, glass apparatus, turbine mixer sample treatment.
A) 25g (being accurate to 0.01) sample is weighed, is placed in the triangular flask with bead and 225ml dilution, is rotating
It is sufficiently vibrated on formula shaking table 30 minutes (200r/min), that is, is configured to the diluted bacteria suspension of 1:10.
B) the diluted bacteria suspension of the above-mentioned 1:10 of 1ml is drawn with aseptic straw, is added into 9ml dilution, is mixed into 1:100
Diluted bacteria suspension successively dilutes and mixes, finally obtains 1:106, the bacteria suspension of the dilutions such as 1:107,1:108 is (according to work
Bacterium number content selects corresponding extension rate), each dilution must replace aseptic straw.
1.3 clostridium butyricum active bacterias count (test tube counting method)
A) it is drawn in 6 test tubes respectively with the RCM culture medium that aseptic straw has melted oneself, each about 15-18mL (can be again
The atoleine of 3mL is drawn in test tube, can not also be drawn), test tube plug beyond the Great Wall, 121 DEG C are spare after sterilizing 30 minutes.
B) before Tube propagation base ot-yet-hardened, different dilution bacterium (outstanding) liquid are drawn respectively with the sterile measuring pipette of 1mL
1mL is added in Tube propagation base, and is gently stirred rapidly so as to be uniformly mixed, test tube plug beyond the Great Wall after sample-adding.
C) each sample takes 3 continuous acceptable diluent degree, and each dilution is repeated 2 times, while making blank pair with dilution
According to cultivating 18-24h in 37 DEG C of constant incubator, 1 DEG C of soil, bacterium colony counts.
Embodiment 3；Saccharomyces cerevisiae
The measurement of saccharomyces cerevisiae is executed by GB/T 22547.
Embodiment 4；The detection of Xylanase activity
It provides to execute by GB/T 23874.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (10)
1. a kind of mixed type the feed for piglet additive, which is characterized in that the mixed type the feed for piglet additive is by micro-
Biology and xylose alcoholase composition；
Microorganism includes bacillus subtilis >=8.5 × 109Cfu/g, clostridium butyricum >=5.0 × 108Cfu/g, saccharomyces cerevisiae >=
1.0×109cfu/g；Xylitol enzyme activity is >=103U/g。
2. mixed type the feed for piglet additive as described in claim 1, which is characterized in that the mixed type the feed for piglet
Function bacterium sum >=10.0 × 10 of additive9cfu/g。
3. a kind of preparation method of mixed type the feed for piglet additive as described in claim 1, which is characterized in that the mixing
The preparation method of type the feed for piglet additive includes:
Step 1: carrying out powder processed for bacillus subtilis, clostridium butyricum, saccharomyces cerevisiae and xylose alcoholase, is uniformly mixed, is mixed
Mould assembly the feed for piglet additive；
Step 2: determination of moisture is carried out to mixed type the feed for piglet additive；Health detection；
Step 3: test to mixed type the feed for piglet additive: exfactory inspection, type inspection are reviewed；
Step 4: label, packaging, transport and storage are carried out to mixed type the feed for piglet additive.
4. the preparation method of mixed type the feed for piglet additive as claimed in claim 3, which is characterized in that the step 1
In, powder obtained, uniform color is consistent, and no agglomeration is smelt without moldy metamorphism and peculiar smell are different.
5. the preparation method of mixed type the feed for piglet additive as claimed in claim 3, which is characterized in that the step 1
In, product grading is all to analyze to sieve by 1.25mm, and 0.80mm analysis sieve oversize is not more than 10%.
6. the preparation method of mixed type the feed for piglet additive as claimed in claim 3, which is characterized in that the step 1
In, mixing should uniformly, and the coefficient of variation (CV) of the uniformity is not more than 7% after tested.
7. the preparation method of mixed type the feed for piglet additive as claimed in claim 3, which is characterized in that the step 2
In, moisture is not more than 10.0%.
8. the preparation method of mixed type the feed for piglet additive as claimed in claim 3, which is characterized in that the step 2
In, sanitary index, total arsenic≤2mg/kg, lead≤8mg/kg, Salmonella must not detect, total number of molds < 4.0 × 104cfu/
g。
9. the preparation method of mixed type the feed for piglet additive as claimed in claim 3, which is characterized in that the step 3
In, exfactory inspection: factory product is responsible for inspection by our company's quality testing department；Inspection project is sense organ, granularity, moisture, function bacterium
Total, small peptide content；It can dispatch from the factory after the assay was approved.
10. the preparation method of mixed type the feed for piglet additive as claimed in claim 3, which is characterized in that the step
In three, type inspection is all events inspection, every year must not be less than primary；For the moment if any following situations, Ying Jinhang type inspection:
When larger change occurs for a) main former, auxiliary material, formula, production technology or equipment；
B) when halt production more than half a year is resumed production again；
C) when exfactory inspection result and last time type inspection result have larger difference；
D) when national departments concerned is spot-check.
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