JP2003289814A - Feed additive agent for physiological activity increase/ growth and development promotion for cultured marine fish using effective microorganism and chinese herb medicine, and method for producing the same - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a feed additive agent for cultured marine fishes using effective microorganisms and Chinese herb medicines. <P>SOLUTION: The feed additive agent for cultured marine fishes using the effective microorganisms and the Chinese herb medicines has a composition ratio as follows; based on water of 1,500 pts. (v/v), 30-50 pts. (v/v) of liquid syrup, 30-50 pts. (v/v) of raw cow milk, 5-15 pts. (w/v) of granules of berries of cactus, 3-8 pts. (v/v) of chitosan oligosaccharide, 2-7 pts. (v/v) of the wood herb liquid of Quercus acutissima, 35-65 pts. (v/v) of the composite fermented liquor of effective microorganisms comprising microorganisms including lactobacillus, a bacterium belonging to the Bacillus, yeast and actinomyces, 10-30 pts. (v/v) of the fermented liquor of a photosynthetic bacterium, and 80-130 pts. (v/v) of an extract of the Chinese herb medicines. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to feed addition for cultured marine fish using effective microorganisms and Chinese herbs and a method for producing the same. More specifically, liquid sugar, raw milk, cactus fruit granules, chitosan oligosaccharides, sapwood sap,
The present invention relates to a feed additive containing a lactic acid bacterium, a Bacillus ((Bacillus bacterium)), an actinomycete, and a complex fermentation broth of an effective microorganism by a microorganism including a yeast, a fermentation broth of a photosynthetic bacterium, and an extract of a herbal medicine.

[0002]

2. Description of the Related Art As of 1997, the production amount of marine fish culture in Korea has increased to 39,121 tons, and marine fish constitutes an important source of human protein. Not only South Korea but also Japan, China, and other countries such as the United States and Europe recently have been trying to improve their diet to prevent adult diseases. The protein's excellence has been certified, and as a result, the amount of fish culture produced and demanded has increased rapidly, demand for improved productivity of fish culture in the international community is intensifying, and competition for its development is intensifying. .

In addition, the demand for marine fish is rapidly increasing due to changes in the dietary habits of Koreans. In order to meet such demand, it is important to supply marine fish by aquaculture. Therefore, the Ministry of Marine Affairs and Fisheries of the Korean government has made a plan to invest heavily in fish farming in its long-term development plan for growing fisheries, and the aquaculture of marine fish tends to increase more and more.

In particular, recently in South Korea, environmental pollution of coastal fishing grounds due to the construction of large-scale seaside industrial parks and the discharge of domestic wastewater has become serious, leading to the occurrence of large-scale red tides and frequent occurrence of diseases of cultured fish. As a result, its productivity is decreasing, but the demand for high-grade fish is increasing due to the development of the national economy.

[0005] Therefore, along with the expansion of fishing grounds by the most advanced aquaculture method, it is further necessary to establish and execute measures for improving productivity. Recently, much research has been done to improve the productivity of cultured fish, but the production of triploids by the genetic engineering method (chromosomal engineering method) from the aspect of biotechnology is still in the practical stage. However, although only growth promotion by growth hormone is partially commercialized and used,
The use of hormones has not attracted the attention of consumers due to safety issues as foods and high cost. In addition to that, Lycium c
honey mille), Korean ginseng, Gomiko (S
chizandara chinensisi Bai
It has also been reported that productivity is improved by using a feed agent such as ll) and chitosan extracted from crab skin for feed addition.

[0006] However, most of the feeds used for aquaculture still use raw feeds such as horse mackerel and mackerel, which are naturally caught, or moist pellets mixed with the feeds. Since (MP) is used, it has many problems such as low efficiency of feed utilization and the possibility of inducing environmental pollution. A revolutionary feed that solves such problems. Development has not been completed yet.

[0007] Therefore, there is a demand for the development of a cheap and high-quality feed through the improvement in the quantitative and qualitative aspects of the feed which accounts for 50% or more of the cost of aquaculture of marine fish.

[0008]

The object of the present invention is to:
In response to the above demands, it includes liquid molasses, raw milk, cactus seed granules, chitosan oligosaccharides, sapwood sap, complex fermentation liquor of active microorganisms, fermented liquor of photosynthetic bacteria, extract of Chinese herbal medicine A liquid feed additive for cultured marine fish and a method for producing the same.

[0009]

[Means for Solving the Problems] As a result of intensive studies on the above-mentioned problems, the present inventors have found that by using an additive containing an effective microorganism and an extract of a herbal medicine, The present invention was completed by discovering that it has the effect of increasing the resistance of fish to hypoxia, the effect of increasing resistance to environmental stress, the effect of reducing pathogenic microorganisms, the effect of increasing vitality due to an increase in physiological activity, and the effect of increasing body weight.

The composition of the present invention comprises water, liquid molasses, raw milk,
Cactus fruit granules, chitosan oligosaccharides, Kunugi tree herb liquor, complex fermentation liquor of effective microorganisms, fermentation liquor of photosynthetic bacteria, and a homogeneously mixed solution, and ginseng, our ▼ Jiju (Ang.
rice gigas Nakai), river ▼ bow ▲ (C
aidium officinale Makin
o), peony (Paeonia albiflorapa)
llasvar. trichocarpa bang
e), Rehmannia glutinos
a Liboschitz var. purpure
a Makino), Shiroyaku (Atractyrodes)
macrocephale Koidzumi), Hui ▼ (Poria cocos Wolf.), Yellow ▼
Mr. ▲ (Astragalus membranceu
s Bunge), meat cinnamon (Cinnamomum ca)
ssia Blume), licorice (Glycyrrrhiz)
a uralensis Fischer et. D
e Candole), Ziangi (Zingiber o)
fficinale Roscoe), Oju (Zizy
pus jajuba Miller Var. in
termis Rehd. ), Citrus n
obiris Makino), Akemiko (Cassia)
tora linne) tea, Kouika (Angelica)
A liquid feed additive for cultured marine fish obtained by mixing and stirring an extract of a Chinese herb medicine prepared by mixing Koreana Maximowicz) with water and then boiling and roasting the mixture, and a method for producing the same. And.

The structure of the present invention also has a water content of 12%.
It is a powdered feed additive for cultured marine fish in which the following compounded feed for cultured marine fish and the liquid feed additive for cultured marine fish described below are mixed.

According to the constitution of the present invention, further, the usual mixed feed for cultured marine fish and the above-mentioned liquid feed additive for cultured marine fish are thoroughly mixed and stirred, and then the mixture is packaged in a vacuum.
Cultured marine fish using effective microorganisms and Chinese herbs produced by fermenting and aging in an automatic temperature-controlled fermentation storage room, heating and drying at 40 to 45 ° C using infrared light while stirring A powder feed additive for use and a method for producing the same.

A liquid feed additive for aquaculture marine fish using the effective microorganisms and Chinese herbs according to the present invention and a method for producing the liquid feed additive will be described in detail.

The liquid feed additive according to the present invention comprises water 15
Liquid molasses 30-50 parts (v / v) against 00 parts (v / v)
v), raw milk 30-50 parts (v / v), cactus fruit granules 5-15 parts (w / v), chitosan oligosaccharide 3-8 parts (v / v), Kunugi tree herb solution 2- 35-65 parts (v / v) of a combined fermented liquid of effective microorganisms (specific gravity 1) including 7 parts (v / v), lactic acid bacteria, Bacillus bacteria, yeast, and actinomycetes
v), 10-30 parts of fermented liquid of photosynthetic bacteria (specific gravity 1) (v /
v) and 80-130 parts (v / v) of Chinese herbal medicine extract
Composition ratio.

Extract of Chinese herbal medicine is 200 parts of water (v / v)
To Korean Ginseng 1-3 parts (w / v), this ▼ return ▲ (Angel
ice gigas Nakai) 1-3 parts (w /
v), river ▼ bow ▲ (Caidium official)
e Makino) 0.5-2 parts (w / v), peony (P
aeonia albiflorapallasva
r. trichocarpa Bunge) 0.5-2
Part (w / v), Rehmannia glut
inosa Liboschitz var. pur
purea Makino) 0.5-2 parts (w / v),
Shiroaku (Atractyrodes macroceph)
ale Koidzumi) 0.5-2 parts (w / v),
Hui ▼ (Poria cocos Wolf.) 0.
5-2 copy (w / v), Mr. Huang ▼ (Astragalus
membranaceus Bunge) 0.5-2
Part (w / v), meat cinnamon (Cinnamomum cass)
ia Blume) 0.5-2 parts (w / v), licorice (G
lycyrrrhiza uralensis Fisc
her et. De Candle) 1-3 parts (w / v), Zingiber officin
ale Roscoe) 0.5-2 parts (w / v), Ojumu (Zyiffus jajuba MillerVa)
r. intermis Rehd. ) 1-3 parts (w /
v), Chen peel (Citrus nobilis Maki)
No) 0.5-2 copies (w / v), Akiko (Cassia)
tora Linne tea (fruit) 1-4 parts (w /
v), Kang Kee (Angelica Koreana Ma)
ximowicz) 0.5-2 parts (w / v), and was obtained by extracting by boiling in water.

The complex fermentation broth of the effective microorganism according to the present invention is
2% milk and 2% molasses were added to distilled water, and the medium sterilized by stirring and heating at 80 ° C. for 40 minutes to sterilize the culture medium of pure rice lactic acid bacteria as shown in the following (1) to (3).
3%, yeast culture 0.1%, actinomycete culture 0.01
%, 0.1% of Bacillus culture fluid,
The cells were cultured at 35 ° C. for 150 hours in the absence of contact with air.

Here, in the above steps (1) to (3), as a culture solution of lactic acid bacteria, 3% molasses is added to milk and the temperature is set to 80 ° C.
The lactic acid bacteria were inoculated and sealed in a liquid medium that had been sterilized by heating with stirring for 40 minutes, and the mixture was cultured at 40 ° C. for 20 hours. Here, the lactic acid bacterium is not particularly limited, but known Streptococcus lactis (Str
eptococcus lactis) or Lactobacillus bulgaricus (Lactobaci)
It is suitable to use illus bulgaricus). The yeast culture solution is 10% distilled water.
The liquid medium sterilized by adding molasses and 10% beer was inoculated with yeast, sealed, and then cultured at 35 ° C. for 20 hours. The yeast is not particularly limited, but the known Saccharomyces cerevisiae (Sacc
It is suitable to use Haromyces cerevisiae or Candida sake. As a culture solution of Bacillus, 5% tofu and 10% brown rice vinegar were added to distilled water, left to stand for 3 hours, boiled, and then cooled and filtered at room temperature, and 1% molasses and 0% were added. Add 5% salt,
A liquid medium that has been sterilized by adjusting the pH to 7.0 to 7.4 is inoculated with Bacillus bacterium, and stirred and cultured at 35 ° C. for 80 hours under microaerobic conditions. Here, there is no particular limitation on Bacillus bacteria, but known Bacillus subtilis or Bacillus nut Bacillus.
It is appropriate to use us natto). As a culture solution of actinomycetes, distilled water contains 10% crab skin and 3%
Liquid that has been sterilized by adding 1% molasses and 0.5% sodium chloride to a product obtained by cooling and filtering at room temperature after adding potato and The medium is inoculated with actinomycetes, and the mixture is stirred and cultured at 35 ° C. for 150 hours under aeration conditions. Here, there are no special restrictions on actinomycetes, but Streptomyces griseus
It is appropriate to use.

The above-mentioned microorganism used in the present invention is a known microorganism, which can be used by being separated and identified by a usual separating and identifying method, or a commercially available product can be purchased and used.

The process for producing a liquid feed additive for cultured marine fish using the effective microorganisms and Chinese herbs according to the present invention will be described below.

30 to 50 parts (v / v) of liquid molasses and 30 to 50 parts (v / v) of raw milk to 1500 parts of water (v / v)
v), cactus fruit granules 5-15 parts (w / v), chitosan oligosaccharide 3-8 parts (v / v), Kunugi tree liquor 2-
7 parts (v / v), lactic acid bacterium, Bacillus bacterium, yeast, complex fermentation liquid of active microorganisms including actinomycete (specific gravity 1) 3
5-65 parts (v / v) and photosynthetic bacteria (3.0 × 10 ³ c)
fu / g) fermentation liquid (specific gravity 1) 10-30 parts (v / v)
And were uniformly mixed and stirred to obtain 1615-1725 parts (v / v) of a raw material mixed solution containing effective microorganisms.

In order to obtain an extract of a herbal medicine other than this, 1-3 parts (w) of ginseng for 200 parts (v / v) of water
/ V), this ▼ return (Angelice gigas N
akai) 1-3 parts (w / v), river ▼ bow ▲ (Caidi
um officinale Makino) 0.5-2
Part (w / v), peony (Paeonia albiflo)
rapallasvar. trichocarpa B
0.5-2 parts (w / v), ripe ground yellow (Rehm)
annia glutinosa Liboschit
z var. purpura Makino) 0.
5-2 copy (w / v), Shiroyaku (Atractyrodes)
macrocephale Koidzumi) 0.
5-2 copies (w / v), Hui ▼ (Poria coco)
s Wolf. ) 0.5-2 copies (w / v), Huang ▼ Mr ▲
(Astragalus membranceus
Bunge) 0.5-2 parts (w / v), cinnamon (Cinn)
ammum cassia Blume) 0.5-2
Part (w / v), licorice (Glycyrrhiza ura)
lens Fischer et. De Can
Dolle) 1-3 parts (w / v), Zingib (Zingib)
er officinale Roscoe) 0.5-
2 copies (w / v), Oju (Zyzypus jajub)
a Miller Var. intermis Reh
d. ) 1-3 parts (w / v), Citrus no (Citrus no)
bilis Makino) 0.5-2 parts (w / v),
Kasuko tora linne tea (fruit) 1-4 parts (w / v), Anika (Angelica)
After adding 0.5-2 parts (w / v) of Koreana Maximowicz) and mixing, and making it to 80-130 parts (v / v) by boiling in water for 10 hours or more, at room temperature. Cooled.

The complex fermentation liquor of the effective microorganisms is
Add 2% milk and 2% molasses to distilled water, stir and heat at 80 ° C for 40 minutes, and sterilize, add 3% molasses in milk and stir and heat at 80 ° C for 40 minutes. Lactic acid bacteria, preferably Streptococcus lactis or Lactobacillus (Bacillus), in a sterilized liquid medium.
s) A liquid medium sterilized by adding 0.3% of a lactic acid bacterium culture solution inoculated with Bulgarix and sealed and then cultivated at 40 ° C. for 20 hours, and 10% molasses and 10% beer to distilled water. Inoculate with yeast, preferably Saccharomyces cerevisiae or Candida sac, and seal at 35 ° C.
0.1% of the culture solution of yeast which was cultured for 20 hours in
After adding 5% tofu and 10% brown rice vinegar to distilled water and allowing to stand for 3 hours to boil, 1% molasses and 0.5% sodium chloride were added to the mixture which was cooled and filtered at room temperature to pH 7.0. ~
Bacillus (Bacillus), preferably Bacillus subtilis or Natto, was inoculated into a liquid medium that had been sterilized by adjusting it to 7.4, and the mixture was cultivated with stirring under microaerobic conditions at 35 ° C for 80 hours. ) Bacteria culture 0.01
%, 10% crab skin and 3% potato in boiling water, boiled, and then cooled and filtered at room temperature to which 1% molasses and 0.5% salt were added to obtain a pH of 7. 0
A liquid culture medium of actinomycetes prepared by inoculating actinomycetes, preferably Streptomyces glycy, into a liquid medium sterilized by adjusting to ˜7.2 and stirring-cultured at 35 ° C. for 150 hours under aerated condition 0.01 %, And the mixture was sealed and cultured at 35 ° C. for 150 hours.

The fermented liquor of the photosynthetic bacterium is obtained by adding 50% of fish inner broth and 10% of seaweed to distilled water, boiling and then cooling and filtering at room temperature to pH 6.8 to 7.
Photosynthetic bacteria in liquid medium sterilized by adjusting to 2
It is preferably produced by adding Rhodesudomonas speriodes, sealing the mixture, and culturing at 40 ° C. for 200 hours under fluorescent illumination.

After mixing 1615-1725 parts (v / v) of the raw material mixed solution containing the effective microorganisms thus obtained and 80-130 parts (v / v) of the Chinese herb extract, the mixture was stirred for 2 hours. , This is stored for 35 to 40 days while stirring and mixing every 20 days at 20 to 35 ° C for 5 hours, and fermented and aged so as to emit a light Chinese herbal medicine and have a light reddish black brown color. A liquid feed additive for cultured marine fish was produced using effective microorganisms and Chinese herbs.

The liquid feed additive for cultured marine fish using the thus produced effective microorganisms and Chinese herbs according to the present invention is placed in a closed container and stored in the shade while avoiding direct rays. The liquid feed additive for cultured marine fish according to the present invention stored in a closed container can be used for about 12 months. After opening the storage container, it is desirable to use it as soon as possible, and it is appropriate that the liquid feed additive left after use is sealed and stored.

The composition of the feed additive for the powder for cultured marine fish using the effective microorganism and the Chinese herb medicine according to the present invention and the method for producing the same will be described below.

The powdered feed additive has a water content of 12
Liquid feed additive 70- according to the present invention to 400 parts (w / v) of a compounded feed for ordinary cultured marine fish of 70% or less
The composition ratio is 90 parts (v / v).

The process for producing a feed additive for a powder for cultured marine fish using the effective microorganisms and Chinese herbs according to the present invention will be described below.

While stirring 400 parts (w / v) of a general mixed feed for cultured marine fish having a water content of 12% or less, stirring 70-90 parts (v / v) of the liquid feed additive according to the present invention. To the mixed feed for fish culture, so that the water content of the mixed feed becomes 25 to 28%, and the obtained mixed feed for fish culture having a water content of 25 to 28% is obtained. After wrapping it in the air, put it in a fermentation storage room with an automatic temperature control adjusted to 35 to 45 ° C., to promote the growth of microorganisms and the fermentation action mutually, and to promote the growth between substances, it is fixed for 50 to 55 days. The temperature is maintained, and the odor of dried fish, the odor of acidity and the smell of alcohol are mixed so that the powder has a blackish brown color, and the mixture is fermented and aged. The fermented and aged powder has a water content of 15
40 to 40% using infrared light while stirring until it becomes less than 40%
It is manufactured by heating to 45 ° C. and drying.

A powder feed additive for aquaculture marine fish using the thus produced effective microorganisms and Chinese herbs is
After carrying out a proper amount of wrapping, store in a well-ventilated shade. The powdered feed additive according to the present invention stored in a closed state can be used for about 12 months. After opening the bag, it is desirable to use it as soon as possible, and it is desirable to store the powdered feed additive according to the present invention that has been left after use in a hermetically sealed state.

[0031]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, a feed additive and a method for producing the same according to an embodiment of the present invention will be described in detail with reference to Examples. However, the present invention is in no way limited by the description of the examples provided herein.

[0032]Example 1  Manufacture of liquid feed additives Step 1 Manufacturing process of raw material mixed solution containing effective microorganisms See Figure 1 1. Volume 200 with a diameter of 60-70 mm and a lid
Disinfectant drugs were added to a 0 liter plastic container
Add 1500 liters of raw water (drinking water). 2. Add 40 liters of liquid molasses and mix and stir. 3. Add 40 liters of fresh raw milk and mix and stir. 4. Add 10 kg of cactus fruit granules and mix and stir
It 5. Liquid chitosan oligosaccharidebodyAdd 6 liters and mix
Stir. 6. Kunugi tree grass liquidbodyAdd 5 liters and mix
Stir. 7. Lactic acid bacteria (4.1 x 10^Threecfu / g), Bacillus
(Bacillus) fungus (1.5 × 10^Twocfu / g), yeast
(2.0 x 10^Twocfu / g), for microorganisms including actinomycetes
50 liters of complex fermentation liquid (specific gravity 1) of effective microorganisms
In addition, mix and stir. 8. Fermentation liquid of photosynthetic bacteria (3.0 × 10^Threecfu / g) 2
Add 0 liters, mix and stir to contain effective microorganisms
1671 liters of raw material mixed solution was produced

The complex fermentation liquor of the effective microorganisms was prepared by adding 2% milk and 2% molasses to distilled water and adding 40% at 80 ° C.
Add 3% molasses to milk to the sterilized medium by stirring and heating for 30 minutes, and inoculate Streptococcus lactis or Lactobacillus bulgaricus on the liquid medium that was sterilized by stirring and heating for 40 minutes at 80 ° C. Saccharomyces was added to a liquid medium sterilized by adding 0.3% of a lactic acid bacterium culture solution that had been sealed and then cultivated at 40 ° C. for 20 hours, and 10% molasses and 10% beer to distilled water.
After inoculating S. cerevisiae or Candida sycae and sealing, yeast culture cultivated at 35 ° C. for 20 hours.
1%, 5% tofu and 10% brown rice vinegar were added to distilled water, left to stand for 3 hours and boiled, then cooled and filtered at room temperature, and 1% molasses and 0.5% salt were added. Bacillus subtilis or Bacillus natto is inoculated into a liquid medium that has been sterilized by adding and adjusting the pH to 7.0 to 7.4.
Bacillus (Ba
cillus) 0.01% of culture solution, distilled water with 10% crab skin and 3% potato, boiled, and then cooled and filtered at room temperature, 1% molasses and 0.5% Of Streptomyces glycy was inoculated into a liquid medium that had been sterilized by adjusting the pH to 7.0 to 7.2 by adding sodium chloride to Streptomyces glycy and cultivated with actinomycetes at 35 ° C. for 150 hours with stirring under aeration conditions. The solution was added with 0.01% of the liquid, sealed, and cultured at 35 ° C. for 150 hours to manufacture.

The fermented liquor of the photosynthetic bacterium is obtained by adding 50% of fish inner broth and 10% of seaweed to distilled water, boiling and then cooling and filtering at room temperature to pH 6.8 to 7.
To the liquid medium, which had been adjusted to 2 and sterilized, Rhodosdomonas speriodes was added, sealed, and cultured at 40 ° C. for 200 hours under fluorescent illumination to produce.

Step 2 Process of Chinese herbal medicine extract and fermentation aging See FIG. 1. 2.1 parts of Korean ginseng (w /
v) and this ▼ return (Angelice gigas N
akai) 2 copies (w / v), and the river ▼ bow ▲ (Caidiu)
m officinale Makino) 1 part (w /
v) and peony (Paeonia albiflorap)
allasvar. trichocarpaBung
e) 1 part (w / v) and ripe ground yellow (Rehmannia)
glutinosa Liboschitz var.
purpurea Makino) 1 part (w / v)
And Shiroaku (Atractyrodes macroce)
1 part (w / v) of Phare Koidzumi and 1 part (w of Poria cocos Wolf)
/ V) and Mr. Huang ▼ (Astragalus memb
ranaceus bunge) 1 part (w / v) and cinnamon (Cinnamomum cassia Blum)
e) 1 part (w / v) and licorice (Glycyrrhiza)
uralensis Fischer et. De
Candole) 2 parts (w / v) and Zinkan (Zin
girber officinale Roscoe) 1
Part (w / v) and Ojumuro (Zyzypus jajub)
a Miller Var. intermis Reh
d. ) 2 copies (w / v) and Cinrus nob
iris Makino 1 part (w / v), Cassia tora linne tea (fruit) 3 parts (w / v), and Angelica Korean
a Maximowicz) 1 part (w / v) and 200
Pour into 1 liter of fresh water and mix. 2. Put the product in a water bath (in which you roast things) 1
It is adjusted to 100 liters by boiling in water for 0 hours or more, and then cooled at room temperature to produce an extract of Chinese herbal medicine. 3. 100 liters of Chinese herb extract produced and raw material mixture 167 containing effective microorganisms produced in the first step
1 liter and 1 liter are put in a plastic container and mixed and stirred for 2 hours. 4. Mixed solution of the obtained raw material mixture and Chinese herbal medicine extract 1
771 liters and 35-35 while stirring and mixing for 1 hour every 5 days in a fermentation chamber kept at 20-35 ° C.
It is stored for 40 days, fermented and aged so that it emits a light Chinese herbal medicine fragrance, and becomes a state of light reddish blackish brown color, and it is a liquid feed additive for cultured marine fish that uses effective microorganisms and Chinese herb medicine , And hereinafter referred to as "Uoiki Izumi").

[0036]Example 2  Manufacture of powdered feed additives See Figure 3 1. Fish produced and sold by the South Korean seawater aquaculture cooperative
Marinepia, a compounded feed for aquaculture (trade name, water
The minute content is 12% or less. ) Rotating 400 kg
Open type electric stirrer (rotation speed is 70-80 / min)
Yes, the capacity is 600 liters. ). 2. Fish spring 7 filled in a plastic storage container
Electric injection machine (pump output 1Hp)
Is used as a universal feed for mixed feed for fish farming in an agitator.
Sprayed so that the water content was 25-28%
Then, mix and stir. 3. Put this in a plastic bag that can hold 40 kg and
After wrapping with a ring wrapping machine, 35-45 ℃
Put in the fermentation storage room with automatic temperature control adjusted to. 4. Facilitates the growth of microorganisms and fermenting multiple microorganisms with each other
To promote use and to enhance the growth of the substance
At a constant temperature (for example, 35 to 45 ° C) for 50 to 55 days
Maintains the scent of dried fish, odor of acidity and smell of alcohol, and
Mixed fermentation and aging so that the color appears black-black-brown
Let 5. Fermented and aged powder about 300-400k
g into an electric stirrer to reduce the water content to 15% or less.
Using an infrared lamp etc. while stirring until
Heat it to 5 ℃ and dry it.
Powdered feed additive for cultured marine fish ("Korean fountain" and life
It is called "Korean fountain". ) Was manufactured.

[0037]Test example 1  Of the composition of fish power spring and oriental spring
test 1. Analysis of main components and main bioactive substances of Chinese medicine Analysis of the main components of Chinese herbs added to Korean spring
Analysis of the main bioactive substances of syrup
1 and Table 2. The analysis is a Korean national recognition tester
I entrusted it to the Science and Technology Analysis Center Co., Ltd. Korea
Table 1 shows the analysis results of the main components of Chinese herbs added to Hosen.
Show.

[Table 1] The glycyridine contained in Uoiki-sen is licorice (Gl
ycyrrhizauralensis Fische
ret. It is a main component of De Candolle) and has anticancer effect, detoxification and physiological activity. Flavonoids were extracted from Jeju cactus berry extract as a substance for inhibiting the oxidative effect and coloring meat quality. Chitosan is a component known to exhibit various physiological effects such as antibacterial effect, and acetic acid was added to wood sap extracted from Kunugi tree. Table 2 shows the results of analysis of major physiologically active substances in Korean traditional spring and fish power spring.

[Table 2]

2. Comparative test of constituents of Gyohosen, Kampoquan, and Marinepia See Tables 3, 4, and 5 In order to compare the compositional components of Kampoquan, Gyokokusen, and Marinpia, we commissioned the Science and Technology Analysis Center Co., Ltd. The analysis results are shown in Tables 3-7. In addition, Korean fountain is made by adding fish power spring to Marinepia and fermenting it.
Overall, the protein, fat, ash, carbohydrates and minerals are almost the same as the marine pia feed. Most of the fish springs containing no marine pier consist of water and contain a small amount of carbohydrates and iron (see Table 3). Table 3 shows the constituents of Korean traditional spring, fish power spring, and marine pier
Shown in.

[Table 3] The composition of amino acids is 3.7 per 100g for Marinepia.
The amount was 7 g, the Korean spring was 3.97 g per 100 g, and the fish force spring was 167.49 ppm per 100 g, showing no significant difference in amino acid components between Marinepia and Korean spring (see Table 4). However, the content of free amino acids in Korean fountain was three times higher than that of Marinepia (see Table 5). Marinepia, oriental fountain,
Table 4 shows the amino acid composition of the fish power spring.

[Table 4] In addition, Table 5 shows the composition of the components of the free amino acids of Marinepia, Korean traditional spring, and Goriki spring.

[Table 5]

See Table 6 The results of vitamin analysis are shown in Table 6. As a result, the total amount of vitamins was reduced. However, the amount of vitamin B ₂ involved in radiant energy and resistance to diseases increased, and folate and choline tended to increase. Table 6 shows the vitamin composition of marine pier, oriental spring and fish power spring.

[Table 6]

The composition of the fatty acids shown in Table 7 was almost all lower fatty acids having short chains in the fish spring (see Table 7). The fatty acid of such a fish spring can enhance fluidity in the biological membrane and participate in physiological functions in the body. Table 7 shows the fatty acid composition of marine pier, oriental fountain and fish power spring.

[Table 7]

3. Microbial constituents of Korean traditional spring and Uoriki spring In order to investigate the composition of effective microorganisms contained in Korean traditional spring and Uoiki spring, analysis was commissioned to Science and Technology Analysis Center Co., Ltd., and the results are shown in Table 8.

[Table 8] The total number of bacteria was 5.6 × 10 ⁸ cfu / g for Korean herbal spring and 3.0 × 10 ⁸ cfu / g for fish power spring (Table 8).
reference).

Further, micrographs of the effective microorganisms isolated from Uoiki-sen and Korean-style spring are shown in FIGS.

[0043]Test example 2  The fish power spring and the oriental spring are added.
Of the effect of agar on the physiological activity of marine fish See Figures 14-30 1. Flounder growth due to the addition of fish power spring and oriental spring
Experiment of length change Experiments on growth changes were conducted using live feeds, which are mainly made of horse mackerel and mackerel.
Control area where fish power spring and oriental spring are not added to the price (comparison
Example) and a mixture of fish power spring and Korean traditional spring is added to the raw feed.
0.3%, 0.6% and 0.9 respectively for the amount
Performed separately in three experimental plots (Examples) in which
It was The evaluation items are the total length (body length) and body of the fish measured by month.
The change with the weight is adopted. As a result, the total length (length) of the fish is
After feeding for 6 months with the addition of Gyoiki-sen and Kampo medicine
The difference in their growth is shown. In January, 0.6% addition area
(Example) achieved the highest growth rate of 31.3 cm
There is. In the control section (comparative example), the lowest performance was 29.6 cm.
Shows the length. In the 0.6% addition group (Example), the control
Compared with the ward (comparative example), the result showed a 6% increase in total length.
(See Figure 14).

With respect to the body weight, the highest level of 375.8 g was achieved in the addition group of 0.3% (Example). And 0.6
% Addition group (Example), 0.9% addition group (Example), and control group (Comparative example). The 0.3% addition group (Example) achieved about 15% increase in body weight as compared with the control group (Comparative example) (see FIG. 15). Although there was no significant difference in the degree of obesity between the addition group (Example) and the control group (Comparative example), the addition group of the mixture of Gyoryu-sen and Hanho-sen (Example)
Showed a higher value than the control (comparative example) (see FIG. 16).

2. Lymphosistis infection occurred in November in the middle of experimental aquaculture of the effect of adding fish force spring and oriental spring on flounder survival. The survival rate at this time is as shown in FIG. In the control section (comparative example), a large amount of deaths due to lymphocystis occurred, and 374 deaths occurred within one month. In the 0.3% addition group (Example) in which 0.3% of the mixture of Gyoryu and Korean traditional spring was added, 243 fish were added, and 0.6% was added to 0.6.
Deaths occurred in 148 fish in the% addition group (Example) and 55 fish in the 0.9% addition group (Example) added with 0.9%. As shown in the above results, the mortality rate due to the occurrence of Lymphocystis decreases as the concentration of the mixture of Gyoriki-sen and Hanho-sen is increased. On the other hand, the number of deaths in one month after the occurrence of the disease subsided is as shown in FIG. 67 fish in the control group (Comparative Example) and 72 fish in the 0.3% addition group (Example) and 0.6% addition group (Example)
In the tail, 0.9% addition group (Example), 57 deaths occurred, and the mortality rate in a stable state after the occurrence of the disease subsided was not significantly different in all the experimental groups. It was

3. Experimental study on changes in physiological activity of Japanese flounder to hypoxic environment by addition of mixture of Gyoiki spring and Korean traditional spring. Liver weight index of Japanese flounder treated with the mixture of fish spring and Korean traditional spring was 0.3%. The highest value is shown in (Example), and even in the 0.6% addition group (Example), the control group (Comparative example)
The value was significantly higher than that (P <0.05, see FIG. 19). From these results, it is shown that the use of the mixture of Gyoiki-sen and Korean-style spring increases the liver weight, and it is presumed that there is an effect of increasing liver activity. However, the amount of protein in hepatocytes in the control group (comparative example) showed the highest value (Fig. 20).
reference). This is because the mixture of fish power spring and Korean herbal spring (Example) has an action in the liver different from that of the control group (Comparative Example), thereby promoting the secretion of the protein synthesized from the liver. It suggests.

On the other hand, the erythrocyte count in the addition group of the mixture of Gyoiki-sen and Korean-style spring (Example) was significantly higher in the addition group of 0.6% (Example) and increased by about 40% (Fig. 21). An increase in red blood cells is a survey item that is directly linked to an increase in vitality of fish, and an increase in oxygen carrying capacity is expected. The increase in oxygen carrying capacity has the effect of reducing the stress caused by the transportation of cultured fish, and can increase the vitality of the fish body. The area of erythrocytes tended to increase with the increase of the number of erythrocytes by the addition of the mixture of Gyoryokusen and Kampoquan.
reference). Here, it should be noted that there was no difference in the number of red blood cells in the 0.3% addition group (Example) and 0.9%.
This is the fact that the red blood cell area in the addition section (Example) increased. The volume ratio of erythrocytes showed a similar tendency, and the result was shown to increase with the addition of the mixture of Gyorinsen and Kampoquan (see FIG. 23).

From the above results, it can be seen that the group to which the mixed agent of Goriki spring and Korean traditional spring (Example) has a higher resistance to hypoxia than the control group (Comparative example). Therefore, in this experiment, the survival time due to fish exposure in the air was examined to investigate the tolerance to hypoxia. The result is shown in Figure 2.
As shown in FIG. 4, the 0.6% addition group (Example) survived for the longest time, the 0.3% addition group (Example) followed by the 0.9% addition group ( Example).
From these results, it can be said that the addition of the mixture of Gyoriki-sen and Kampo-en has increased resistance to hypoxia.

4. Experimental study on changes in liver function of Japanese flounder due to the addition of a mixture of Gyorin spring and Korean oriental spring. Liver function is an item that is often used by the human body as an index of health. In this experiment, basic blood proteins and GOT and GPT, which are indicators for measuring liver function, were measured and differences in liver function were analyzed. As a result, the protein level in the blood was higher in all the groups (Examples) to which the mixed agent of Gryokusen and Korean traditional spring was added (see FIG. 25) than in all the control groups (Comparative Example).
The increase in blood protein means that the activity of liver function is high, and it was the highest in the 0.6% addition group (Example).

GOT is the opposite, and in the 0.6% addition group (Example), 14.24 carmen (karme).
n) / ml (lowest value), which was significantly lower in the 0.3% addition group (Example) than in the control group (Comparative Example) (see FIG. 26). From such a result, 0.6
It can be said that the highest liver function is exhibited in the% addition group (Example).

On the other hand, regarding GPT, no activity was detected in the control group (comparative example) and the 0.3% added group (example), and 0.31 in the 0.6% added group (example). Carmen / m
1 (see FIG. 27). This result is a very exceptional phenomenon, which may be because the GPT detection kit (kits) used was for humans.
For more accurate changes in physiological activity, more specific and detailed deliberation should be conducted, but if the above results are comprehensively judged, 0.6% addition group (Example) It seems to have higher activity in liver function than other experimental plots.

5. Experimental study on changes in SOD and CAT activity of flounder due to addition of mixture of Gyoiki-sen and Korean-style spring. Active oxygen means a chemical substance containing oxygen and having high reactivity. In a narrow sense, superoxide anion radical (s
upper oxide anion radical)
(.O ₂ ⁻ or O ₂ ⁻ ), hydrogen peroxide (H ₂ O ₂ ),
Hydroxyl radical
al) (.OH) etc. are included. It is known that such active oxygen is generated by stress and has various physiological adverse effects such as gene mutation. In order to avoid the damaging effect of active oxygen, SOD (su
peroxidid dismutase) and CAT (c
It produces and secretes enzymes such as atalase). Therefore, in this experiment, SOD and C which have physiological anti-defense action
The activity of AT was measured. As a result, SOD showed the highest activity in the 0.6% addition group (Example) (Fig. 2).
8). It has a function of converting superoxide anion radicals (.O ₂ ⁻ or O ₂ ⁻ ) generated in the living body into hydrogen peroxide (H ₂ O ₂ ), and is a series of processes for detoxifying harmful chemical substances. Has been shown to promote. Further, CAT shows a high value in the 0.3% addition group (Example) and the 0.6% addition group (Example), and accelerates the process of converting hydrogen peroxide generated by SOD into non-toxic water. Suggesting that it is possible (see FIG. 29).
Summarizing the above results, it is expected that the addition of the mixture of Gyoryu and Kampo spring will improve the function of protecting the fish body from harmful active oxygen, and such a mechanism of protection is more resistant to environmental stress. To bring about the production of fish.

6. Changes in pathogenic microorganisms in raw feed for flounder due to addition of mixture of Gyoriki and Korean herbal spring Most of the raw feed used in experimental farms depend on import and its management status Has a great influence on the production amount and the health of the fish body. Further, the raw feed also contains a large amount of pathogenic microorganisms, and it is considered that the raw material itself causes a disease. Therefore, in this experiment, the moist pellets (M
P) The number of three representative pathogenic microorganisms in feed was grasped and the effect of reducing pollutants was investigated. As a result, as shown in Table 9, a large amount of edwadsierra tarda (Edw) was added to the raw feed (control group) prepared by adding general commercial feed.
ardsiella tarda), Vibrio anguillarum
And Strebto Cox
coccus sp. ) Was detected. Here, as a result of adding the mixture of Gyoiki-sen and Korean-style fountain, in the 0.3% addition group (Example), 2% of the control group (Comparative example) and 35.9% were obtained.
% And 50% levels have been obtained. 0.6%
In the addition section (Example), it decreased to 1%, 13%, and 39.6%, and in the addition section (Example), 36.4%, 3%
It decreased to 0.3% and 31.0%. From the above results, it can be seen that the addition of the mixture of Gyoriki-sen and Kampo-sen is effective not only for increasing the physiological activity but also for improving the quality of the feed. Table 9 shows the change in the pathogenic microflora in the raw feed due to the addition of the mixture of Gyoiki-sen and Kampo-sen.

[Table 9]

7. Experiments on environmental changes in running-water type and circulation filtration type aquaculture tanks due to the addition of the mixture of Gyoiki spring and Korean traditional spring. 10 and Table 11 respectively. As a result, it was determined that there was no change in the water quality due to the use of the mixture of fish-powered springs and Korean traditional springs containing effective microorganisms. FIG. 30 shows the results of observing the effect on water quality by adding 1% of fish spring to a circulating filtration type water tank. As a result, the composition of nitrogen and phosphorus in the breeding water did not show a significant difference from the control (comparative example). From the above facts, it can be seen that there is no significant change in the water quality due to the addition of the mixture of Goriki spring and Korean traditional spring. However, the mixture of Gyoryokusen and Korean oriental spring contains a large amount of various anaerobic or aerobic bacteria, which may be effective in improving the water quality of the circulation filtration type. . Table 10 shows changes in water quality in the running-water aquaculture tank.

[Table 10] Table 11 shows the change in water quality of the discharged water from the running water type farm.

[Table 11]

[0055]

[Effects of the Invention] As described above, a feed containing the additive according to the present invention (a feed additive for increasing physiological activity and promoting growth and development of cultured marine fish using effective microorganisms and Chinese herbs) is used. The effects obtained by aquaculture are as follows. It has the effect of increasing the resistance to hypoxia that occurs during the transportation of cultured fish, and facilitates the transportation of live fish. It has the effect of increasing the amount of antioxidant enzymes in the body, and has the effect of increasing the resistance to environmental stress. It is expected that the increase in vitality can be expected due to the decrease in the amount of bacteria, and the effect of reducing the pathogenic microorganisms contained in the raw feed used for moist pellets can be obtained, and the preventive effect against the cause of disease infection in cultured fish can be obtained. For example, the activity is increased, the vitality increasing effect is obtained, and the fish body weight increasing effect is obtained.

[Brief description of drawings]

FIG. 1 is a manufacturing process chart showing a first process of a method for manufacturing a fish spring according to a first embodiment of the present invention.

FIG. 2 is a manufacturing process diagram illustrating a second process of the method for manufacturing a fish spring according to the first embodiment of the present invention.

FIG. 3 is a manufacturing process diagram illustrating a method of manufacturing Korean fountain according to Example 2 of the present invention.

FIG. 4 is a micrograph of a lactic acid bacterium isolated from a Korean fountain according to Example 2 of the present invention.

FIG. 5 is a micrograph of Bacillus bacterium isolated from Korean traditional spring according to Example 2 of the present invention.

FIG. 6 is a micrograph of yeast isolated from Korean traditional fountain according to Example 2 of the present invention.

FIG. 7 is a photomicrograph of a photosynthetic bacterium isolated from a Korean fountain according to Example 2 of the present invention.

FIG. 8 is a photomicrograph of Azotobacter isolated from Korean fountain according to Example 2 of the present invention.

FIG. 9 is a micrograph of a lactic acid bacterium isolated from a fish power spring according to Example 1 of the present invention.

FIG. 10 is a micrograph of Bacillus bacterium isolated from a fish spring according to Example 1 of the present invention.

FIG. 11 is a micrograph of yeast isolated from a fish spring in Example 1 according to the present invention.

FIG. 12 is a micrograph of a photosynthetic bacterium isolated from a fish spring according to Example 1 of the present invention.

FIG. 13 is a micrograph of Azotobacter separated from the fish power spring according to Example 1 of the present invention.

FIG. 14 is a graph showing changes in the total length of flounder caused by the administration of a mixture of Gyorinsen and Korean Oriental spring.

FIG. 15 is a graph showing changes in body weight of flounder due to administration of a mixture of Goriki-sen and Kampo-sen.

FIG. 16 is a graph showing changes in the degree of obesity of Japanese flounder, which is caused by administration of a mixture of Goriki spring and Korean traditional spring.

FIG. 17 is a graph showing the survival rate of Lymphocystis infection at the time of infection by the administration of a mixture of Gyorin spring and Korean traditional spring.

FIG. 18 is a graph showing the change in survival rate due to administration of a mixture of Gyorinsen and Korean Oriental spring.

FIG. 19 is a graph showing the liver weight index of flounder 8 months after the administration of the feed additive which is a mixture of Goriki-sen and Kampo-sen.

FIG. 20 is a graph showing the amount of protein contained in the liver of Japanese flounder 8 months after the administration of the feed additive which is a mixture of Goriki spring and Korean traditional spring.

FIG. 21 is a graph showing the red blood cell count of flounder 8 months after the administration of a feed additive which is a mixture of Goriki-sen and Kampo-sen.

FIG. 22 is a graph showing the mean erythrocyte volume of flounder 8 months after the administration of the feed additive which is a mixture of Gyoriki-sen and Koryo-sen.

FIG. 23 is a graph showing the erythrocyte volume ratio of Japanese flounder 8 months after the administration of the feed additive of the mixture of Goriki and Kampo.

FIG. 24 is a graph showing the survival time in the case where the flounder was exposed to the air after 8 months after the administration of the feed additive which was a mixture of Goriki-sen and Koryo-sen.

FIG. 25 is a graph showing the protein in the blood of Japanese flounder 8 months after the administration of the feed additive which is a mixture of Gyorinsen and Kampo Spring.

FIG. 26 is a graph showing the serum GOT activity of flounder 8 months after the administration of the feed additive which is a mixture of Gyorinsen and Korean traditional spring.

FIG. 27 is a graph showing the serum GPT activity of Japanese flounder 8 months after the administration of the feed additive of the mixture of Goriki-sen and Kampo-sen.

FIG. 28: SOD (superox) of Japanese flounder 8 months after the administration of the feed additive which is a mixture of Goriki-sen and Kampo-sen.
Fig. 2 is a graph showing the activity of "ide dismutase".

FIG. 29: CAT (catalas) of Japanese flounder 8 months after the administration of the feed additive which is a mixture of Goriki-sen and Kampo-en.
e) A graph showing activity.

FIG. 30 is a graph showing changes in nitrogen and phosphorus in the circulation filtration type water tank after addition of the mixture of Gyoriki spring and Korean traditional spring.

[Explanation of symbols]

None

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Kim             Republic of Korea             Part 407-403 F-term (reference) 2B005 GA01 HA02 LA07 LB07 MA08                       MB02 MB09                 2B150 AA08 AB02 AC04 AC05 AC11                       AC13 AC15 AC24 AD04 AE13                       AE33 AE42 BB01 CB01 CC11                       CE25 DD32 DD45 DD57

Claims (8)

[Claims] 1. Liquid molasses 30-50 parts (v / v) and raw milk 30-50 parts (v / v) per 1500 parts water (v / v).
v), cactus fruit granules 5-15 parts (w / v), chitosan oligosaccharide 3-8 parts (v / v), Kunugi tree liquor 2-
7-part (v / v), lactic acid bacterium, Bacillus bacterium, yeast, and complex fermentation liquid 35-of effective microorganisms including actinomycete
65 parts (v / v), fermentation liquid of photosynthetic bacteria 10-30 parts (v
/ V) and 80-130 parts of Chinese herbal medicine extract (v /
v) is added with the above composition ratio, and the Chinese herb extract is 1-3 parts (w / v) of Ginseng.
This ▼ Return (Angelice gigas Naka
i) 1-3 parts (w / v), River Bow (Caidium)
Officinale Makino 0.5-2 parts (w / v), peony (Paeonia albiflor)
apalasvar. trichocarpa Bu
0.5-2 parts (w / v), ripe ground yellow (Rehma)
nniaglutinosa Liboschitz
var. pururea Makino) 0.5-
2 copies (w / v), Shiroaku (Atractylodes m)
acrocephale Koidzumi) 0.5-
2 copies (w / v), Hui decree (Poria cocos
Wolf. ) 0.5-2 copies (w / v), Mr. Huang ▼ (As
tragalus membraceus Bun
ge) 0.5-2 parts (w / v), cinnamon (Cinnamo)
um cassia Blume) 0.5-2 parts (w
/ V), licorice (Glycyrrhiza uralen)
sis Fischer et. De Candol
le) 1-3 parts (w / v), Zingiber (Zingiber
officinale Roscoe) 0.5-2 parts (w / v), Otsuki (Zyiffus jajuba)
Miller Var. intermis Reh
d. ) 1-3 parts (w / v), Citrus no (Citrus no)
bilis Makino) 0.5-2 parts (w / v),
Kasuko tora linne tea (fruit) 1-4 parts (w / v), Anika (Angelica)
Koreana Maximowicz) 0.5-2 parts (w / v) of the dried drug, 200 parts of water (v / v)
v) In addition to v), a feed additive for cultured marine fish using an effective microorganism and a Chinese herb medicine characterized by being extracted. 2. The feed additive according to claim 1, wherein the complex fermentation broth of the effective microorganism is 2% milk and 2% in distilled water.
% Of molasses and sterilized by stirring and heating at 80 ° C for 40 minutes and sterilized by adding 3% of molasses into milk and stirring and heating at 80 ° C for 40 minutes and sterilized by lactic acid bacteria. Inoculate and seal, then 2 at 40 ° C
0.3% of lactic acid bacterium culture solution which was cultivated for 0 hours and 1 in distilled water
After the yeast was inoculated into a liquid medium sterilized by adding 0% molasses and 10% beer and sealed, 0.1% of the yeast culture cultivated at 35 ° C. for 20 hours and 5 times in distilled water % Tofu and 10% brown rice vinegar were added, the mixture was left standing for 3 hours, boiled, and then cooled and filtered at room temperature to give a product.
Add 1% molasses and 0.5% salt to give a pH of 7.0
Bacillus (Bacillus) was inoculated into a liquid medium that had been sterilized and adjusted to ~ 7.4, and was cultivated at 35 ° C for 80 hours under agitation under stirring conditions.
Bacterial culture solution 0.01%, distilled water with 10% crab skin and 3% potato were boiled, and then cooled and filtered at room temperature, and 1% molasses and 0.5% Sodium chloride was added, and the liquid medium that had been sterilized by adjusting the pH to 7.0 to 7.2 was inoculated with actinomycetes, and at 1 ° C at 35 ° C.
Add 0.01% of the above-mentioned actinomycete culture solution, which has been stirred and cultured under aeration conditions for 50 hours, and seal.
The product cultivated for 50 hours, wherein the fermented liquid of the photosynthetic bacterium is a product obtained by adding 50% of the inner portion of the fish and 10% of seaweed to distilled water and boiling and then cooling and filtering at room temperature to pH 6. After inoculating the photosynthetic bacteria into a liquid medium sterilized by adjusting it to 8 to 7.2,
A feed additive for cultured marine fish using an effective microorganism and a Chinese herb, which is a product that is sealed and cultured at 40 ° C. for 200 hours under fluorescent illumination. 3. The feed additive according to claim 2, wherein the lactic acid bacterium is one or two lactic acid bacteria selected from Streptococcus lactis or Lactobacillus bulgaris, and the yeast is Saccharomyces cerevisiae or Candida saek, Bacillus is Bacillus subtilis or Bacillus natto, the actinomycete is Streptomyces glycy, and the photosynthetic bacterium is Rhodosdomonas speriodes. Feed additive for cultured marine fish. 4. Liquid molasses 30-50 parts (v / v) and raw milk 30-50 parts (v / v) per 1500 parts (v / v) of water.
v), cactus fruit granules 5-15 parts (w / v), chitosan oligosaccharide 3-8 parts (v / v), Kunugi tree liquor 2-
35-65 parts (v / v) of a complex fermentation solution of effective microorganisms including 7 parts (v / v), lactic acid bacteria, Bacillus bacteria, yeast, and actinomycetes, and photosynthetic bacteria (3.0 × 10 ³ cfu / g)
10-30 parts (v / v) of the fermented liquid of (1) are uniformly mixed and stirred, and a raw material mixed solution 1615-1725 containing effective microorganisms is added.
Part (v / v), 1-3 parts (w / v) of Korean Ginseng, this ▼ Ji (Angeli
ce gigas Nakai) 1-3 parts (w / v),
River ▼ Bow ▲ (Caidium office M
akino) 0.5-2 parts (w / v), peony (Paeo)
nia albiflora pallasvar. tr
ichocarpa Bunge) 0.5-2 parts (w /
v), ripening yellow (Rehmannia Libosch)
itzvar. purpurea Makino)
0.5-2 copies (w / v), Shiroyaku (Atractylod)
es macrocephale Koidzumi)
0.5-2 copies (w / v), Hui decree (Poria co
cos Wolf. ) 0.5-2 copies (w / v), Mr. Huang ▲ (Astragalus membraceus)
Bunge) 0.5-2 parts (w / v), meat cinnamon (Cin)
nammum cassia Blume) 0.5-
2 parts (w / v), licorice (Glycyrrhiza ur
alensis Fischer et. De Ca
1-3 parts (w / v), Zensei (Zingi)
ber officinale Roscoe) 0.5
-Part 2 (w / v), Otsuki (Zyzypus jaju)
baMiller Var. intermis Reh
d. ) 1-3 parts (w / v), Citrus no (Citrus no)
bilis Makino) 0.5-2 parts (w / v),
Kasuko tora linne tea (fruit) 1-4 parts (w / v), Anika (Angelica)
Koreana Maximowicz) 0.5-2 parts (w / v) of a herbal mixture is added to water 20
After adding to 0 part (v / v) and mixing, it is boiled for 10 hours or more to 80-130 parts (v / v) and cooled at room temperature to extract 80-130 parts (v / v) of Chinese herb medicine.
v), and the raw material mixed solution 1615-1725 containing the effective microorganisms
Parts (v / v) and the extract of Chinese herbs 80-130 parts (v
/ V) is mixed and stirred for 2 hours, and then stirred and mixed at 20 to 35 ° C. for 1 hour every 5 days.
~ 40 days storage, fermented and aged to produce a light Chinese herbal medicine aroma and a light reddish black brown color Method. 5. The method for producing a feed additive according to claim 4, wherein the complex fermentation broth of the effective microorganisms is obtained by adding 2% milk and 2% molasses to distilled water and stirring at 80 ° C. for 40 minutes.・
3% molasses was added to the milk that had been sterilized by heating, and the liquid medium that had been sterilized by stirring and heating at 80 ° C for 40 minutes was inoculated with lactic acid bacteria and sealed, then at 2 ° C at 40 ° C.
0.3% of lactic acid bacterium culture solution which was cultivated for 0 hours and 1 in distilled water
Yeast was inoculated into a liquid medium sterilized by adding 0% molasses and 10% beer, sealed, and then cultivated at 35 ° C. for 20 hours at 0.1% yeast solution and 5% tofu in distilled water. And 10% brown rice vinegar were added, left to stand for 3 hours, boiled, and then cooled and filtered at room temperature, and 1% molasses and 0.5
Bacillus (Bacillus) was inoculated into a liquid medium that had been sterilized by adjusting the pH to 7.0 to 7.4 by adding sodium chloride (%) to Bacillus (agitated and cultured at 35 ° C. for 80 hours under microexhalation). Bacillus) bacterial culture 0.01%
Then, 10% crab skin and 3% potato were added to distilled water, boiled, and then cooled and filtered at room temperature, and 1% molasses and 0.5% salt were added to the mixture to pH 7.0. ~
The liquid medium sterilized by adjusting to 7.2 was inoculated with actinomycetes, and the mixture was added with 0.01% of the culture solution of actinomycetes, which was stirred and cultured at 35 ° C. for 150 hours under aeration conditions. The fermentation broth of the photosynthetic bacterium was produced by culturing at 150 ° C. for 150 hours, and after boiling and adding 50% of fish viscera and 10% of seaweed to distilled water, the mixture was cooled and filtered at room temperature to pH 6. After being inoculated into a liquid medium sterilized by adjusting to 8 to 7.2, the photosynthetic bacteria are inoculated, sealed and cultured at 40 ° C. for 200 hours under fluorescent illumination. A method for producing a feed additive for aquaculture marine fish using microorganisms and Chinese herbs. 6. The method for producing a feed additive according to claim 5, wherein the lactic acid bacterium is one or two selected from Streptococcus lactis or Lactobacillus bulgaris, and the yeast is Saccharomyces. S. cerevisiae or Candida saek, Bacillus is Bacillus subtilis or Bacillus natto, the actinomycete is Streptomyces glycy, and the photosynthetic bacterium is Rhodosdomonas speriodes. A method for producing a feed additive for aquaculture marine fish used. 7. 70-90 parts (v / v) of a feed additive produced by the method according to claim 4 with respect to 400 parts (w / v) of a general mixed feed for cultured marine fish having a water content of 12% or less. v) A feed additive for aquaculture marine fish, which contains an effective microorganism and a Chinese herb medicine containing v). 8. A feed additive 70-produced by the method according to claim 4, wherein 400 parts (w / v) of a compounded feed for general cultured marine fish having a water content of 12% or less is stirred.
90 parts (v / v) were uniformly sprayed into the above-mentioned mixed feed for fish farming so that the water content of the mixed feed was 25 to 28%, and the obtained water content was 25 to 28%. 35-45 after wrapping 40 parts (w / v) of the above-mentioned mixed feed for fish culture
Put it in the fermentation storage room with automatic temperature control set at ℃, maintain constant temperature for 50-55 days to promote mutual fermentative action with growth of microorganisms, and promote growth condition between substances. 300-400 parts (w / v) of the fermented and aged powder are mixed and fermented and aged so that a fishy odor, an acid odor and an alcoholic odor are emitted, and the color of the powder is blackish brown.
While stirring until the water content falls below 15%, 40-
A method for producing a feed additive for aquaculture marine fish using a herbal medicine of an effective microorganism, which comprises heating at 45 ° C. and drying.