KR101933075B1 - Feed additives for raising eels - Google Patents

Abstract

The present invention relates to a feed additive for farming freshwater eels. According to the present invention, the feed additive for farming freshwater eels comprises inorganic powder selected from a group consisting of shungite powder, illite powder, zeolite powder, barley stone powder, bentonite powder, diatomite powder, germanium powder, red clay powder, alunite powder, jade powder, and a mixture thereof. The feed additive for farming freshwater eels is based on inorganic and natural materials to prevent a problem caused by use and long-term use thereof and increase growth of eels. Also, the feed additive for farming freshwater eels can increase survival rates of eels by supplying minerals to eels and increasing immunity of eels, and can also prevent and alleviate head ulcer disease common in freshwater eels.

Description

{FEED ADDITIVES FOR RAISING EELS}

The present invention relates to a feed additive for freshwater eel culture. More particularly, the present invention relates to a feed additive for freshwater eel culturing which is capable of preventing and / or improving tofu ulcer disease, which includes inorganic substances mixed in a certain range to help grow freshwater eel.

As a result of economic growth, people's living standards have improved, and meat consumption has increased due to changes in diet patterns, and various adult diseases (hypertension, diabetes, arteriosclerosis, brain and cardiovascular diseases, dementia, etc.) are increasing rapidly each year. In addition, as the general common sense of health increases, the Korean people are making a lot of efforts to protect their health, and it is well known that they are changing from eating habits based on meat to eating culture focused on fish such as fish. Therefore, a method for increasing safe and stable production of aquatic products to the human body should be considered.

On the other hand, efforts to directly or indirectly ingest these natural substances are becoming stronger as natural consumption of natural products exhibiting various physiologically active functions without adversely affecting the human body is increased. Efforts have also been made to convert physiologically active substances from natural materials into highly functional materials with higher functionality. Therefore, it is urgently required to apply biotechnology for development of aquaculture industry such as development of high-functional feed additives using natural raw materials.

However, due to a sharp decline in the production of wild aquatic organisms, most of the aquatic products are supplied and cultivated, and some depend on imports from foreign countries. On the other hand, as a result of the generalization of aquaculture technology and changes in the breeding environment, it is converted into a mass production system, resulting in the occurrence of various diseases and massive deaths. Many antibiotics and medicines are currently on the market, but most of them are artificial synthetic products, which, when absorbed in large quantities in the human body, cause serious side effects and cause environmental pollution. Therefore, it is necessary to find a way to prevent the disease from occurring by enhancing the anti-infectivity by the natural immunity-enhancing action.

There are 16 kinds of eels and 3 kinds of subspecies. There are 19 kinds of eels widely distributed in the world. In Korea, there are two species of eel (Anguilla japonica) and anguilla marmorata Is an eel.

The eel is a descendant fish and the eel which has been raised to the river grows from 300 to 1,000 g for 3 to 12 years to become a sexually transmitted fish, and in the early spring it sprays 7 million to 12 million fishes. Since 1960, the government has been actively promoting eel farming in Korea.

Despite the increase in the export volume of eels, the development of eel-only aquaculture feed additives has not been investigated at all, despite the high proportion of eel culture in inland water culture. Considering this fact, there has been a need for the development of eel aquaculture feedstuffs which have better feed efficiency and prevent disease than high-performance feed additives or imported feeds.

Eel aquaculture feeds contain more protein than any other diets, and in the case of hot and humid summers, the feed itself is highly corrosive (can be preserved for about 1 week) and the disease caused by it is intensified. Long-term feed preservation books should be developed.

Therefore, the development of a high-functional feed additive for aquaculture eel by using physiologically active substances derived from natural materials, which have no side effects, and the improvement of fish resistance by natural immunity enhancement and the improvement of productivity by the action of growth promotion, It is expected that it will create economic profit of.

Currently, artificial synthesis is mostly used to treat fish diseases such as Aeromonas hydrophila, Flexibacter columnaris, Edwardsiella tarda, and Pseudomonas anguilliseptica, which are the main causes of the disease in freshwater eel culture. Medicines such as antibiotics have been used. However, by using these artificial synthetic materials frequently in fish culture, it is possible to cause serious side effects by accumulation in the fish body, and it can become an environmental pollutant source.

In addition, it has a relatively low carbohydrate digestion efficiency compared to digestion of fat and protein due to the characteristics of eel growth. Excessive use of protein content due to excessive competition (over 40%) caused a sharp increase in the consumption of aquaculture feed. However, research and development should be carried out to lower the use of expensive protein and to increase the proportion of soluble carbohydrate, which is relatively low in production cost. Therefore, it is urgently required to develop a high value-added feed that can reduce the production cost and increase the production of the eel by exploring a method for increasing the carbohydrate digestion efficiency of the eel.

It is necessary to develop a new aquaculture additive for environmentally friendly aquaculture and to improve the problems of existing aquaculture feed which is developed without consideration of aquaculture water pollution and dissolved oxygen in the aquatic environment.

Artificial synthetic feeds for aquaculture produce deficient vitamins and minerals, which are essential for growth. In addition, they are added artificially to prevent them, thereby raising the economic burden of producers by increasing the feed price. Therefore, it should be able to be solved through proper application of natural products.

KR 10-2017-0056277 A KR 10-2005-0051432 A

It is an object of the present invention to provide a feed additive for freshwater eel which is based on an inorganic substance and a natural substance and can improve the growth of eels without any problems due to its use and long-term use.

In addition, the present invention aims at enhancing the survival rate of freshwater eel by supplying minerals and enhancing immunity by using the feed additive for freshwater eel so as to prevent and improve tofu ulcer disease which is often caused in freshwater eel.

Another object of the present invention is to provide a method for producing the feed additive.

Yet another object of the present invention is to provide a feed comprising the feed additive for freshwater eel.

In order to achieve the above object, a feed additive for freshwater eel culture according to an embodiment of the present invention includes shungite, fine powder illite, zeolite, fine powdered bentonite, fine powder bentonite, fine powder diatomaceous earth , Fine powder germanium, fine powdered yellow loam, fine powdered dolomite, fine powder jade, and a mixture thereof.

The inorganic powder may have an average particle diameter of 0.005 to 30 탆.

The feed additive for freshwater eel culturing may further comprise a first plant extract selected from the group consisting of apricot tree extract, plum tree extract, cotton extract, and dandelion extract.

The feed additive for freshwater eel culturing may further comprise a second plant extract selected from the group consisting of acorn extract, mandarin orange extract, and mixtures thereof.

The feed for freshwater eel according to another embodiment of the present invention includes the feed additive for the freshwater eel culture.

According to another embodiment of the present invention, there is provided a method for preparing a feed additive for freshwater eel culturing, comprising the steps of: preparing a feed additive for freshwater eel culturing, A dispersion-mixing step of preparing a water-dispersed inorganic material for mixing and dispersing an inorganic powder selected from the group consisting of fine powder, jute and mixture thereof in purified water; And a second plant extract mixing step of mixing the water-dispersed inorganic material with the second plant extract selected from the group consisting of a flower extract, a gangrene extract, and a mixture thereof after the dispersion and mixing step.

The method for preparing the feed additive for freshwater eel culturing may further include a heat treatment step of applying heat treatment to the water-dispersed inorganic material after the dispersion and mixing step.

The method for preparing a feed additive for freshwater eel culture includes a first plant which mixes the first plant extract selected from the group consisting of apricot tree extract, plum tree extract, cotton extract and dandelion extract into the inorganic powder dispersion after the ion treatment step Extract mixing step.

Hereinafter, the present invention will be described in more detail.

According to one embodiment of the present invention, the feed additive for freshwater eel cultivation is selected from the group consisting of shungite, fine powder illite, zeolite, fine powder compactor, fine powder bentonite, fine powdered diatomaceous earth, fine powdered germanium, Fine powders, fine powders, fine powders, jade powders, and mixtures thereof.

The term " fine powder " in the present invention refers to a powder produced by crushing with a ball mill, a jet mill or other grinding apparatus or having a property of a fine powder in the course of synthesis, and the fine powder is defined as having an average particle diameter of 100 m or less.

In the present invention,? Gite is a layer (thickness of 2 m or less) or a lens-like body in a low-denuded shale rich in small graphite microcrystals in the Proterozoic Jatulian in Shunga near Suoj? Rvi in northern Karelia Ladoga, do. It is black like pitch, dense, opaque, metallic luster. It is cold. Hardness 2.5. d 1.75 ~ 1.80, making a thick acid in a thick nitric acid that does not melt in the fire.

In the present invention, the chemical formula is (K, H ₃ O) Al ₂ (Si, Al) ₄ O ₁₀ (H ₂ O, OH) ₂ . In terms of chemical composition, SiO ₂ , MgO, and H ₂ O are relatively abundant and K ₂ O is relatively small although they are close to muscovite. It is a kind of minerals such as dolomite. A hardness of 1 to 2, and a specific gravity of 2.6 to 2.9. The cleavage is complete with [001], streaking color is white, and earthy shine. There is an opinion that it is a mixed mineral containing different components, not an independent mineral in terms of chemical composition or crystal structure. It is produced in aluminum-rich heterogeneous or tuffaceous sedimentary rocks and is produced as altered minerals in hydrothermal ore deposits.

The zeolite referred to in the present invention is also referred to as zeolite. There are many kinds, but they have commonality in the points of high water content, crystal properties, and acidity. The hardness does not exceed 6, and the specific gravity is about 2.2. It is usually colorless or translucent white. This name was attached because it boils when it heats up by the hull. Most types are dissolved in hydrochloric acid and often become glue, but a few kinds do not dissolve in hydrochloric acid. The main types are antipodes, eye glasses, chabazite, soda ash, heulandite, stilbite, romantite, and enesite. It is produced in the cavities and bases of basic igneous rocks such as basalt and tuff tuff, and sometimes exists as a secondary mineral in granite and gneiss. It may also be produced from gold ore or other veins. Crystalline structure of each atom is loosened, so that even if the water filling the space is discharged as a high heat, the skeleton remains, so other fine particles can be adsorbed. This property is used as an adsorbent, and it is used as a molecular sieve (molecular sieve) for separating fine particles of different sizes.

The elvan in this invention is known as the term used in Oriental medicine in China and belongs to the granite class by geological classification. Quartz porphyry, feldspar porphyry, granite porphyry are similar to quartz-mononite in rock names. Quartz and feldspar are closely packed. It was named Mowanstone because it was like a rice ball made of barley rice, in which white, light yellow brown, light gray, dark green, or light green rocks were mixed with red or white dots. Its main components are anhydrous silicic acid and aluminum oxide, and contains a small amount of iron oxide. Known as the predominant white lacquer, it is used as an anti-inflammatory agent to treat skin diseases such as swelling or swelling, which was formerly used to purify pills. According to Dong-bok, the nature is sweet, warm, and poisonous. It is composed of 3 to 150,000 holes per cm ³ , and is strong in adsorption and contains about 25,000 inorganic salts. It is also used as a harmful metal removing agent because it acts to exchange ions with heavy metals. It is known that when heat is applied to this rock, far-infrared rays are emitted. Because of these characteristics, it is used in various industries such as steam room, tableware, medical equipment.

In the present invention, many benzonites include quartz, feldspar, zeolite, and the like. The colors are white, gray, light brown, pale green, and the like. It has a pearly luster and a brass luster. It is produced as dense mass with fat. It is similar to montmorillonite in that it absorbs water and swells, and has a clear cation exchange property. Tuff and vitreous rhyolite have been altered. It is widely used as a main component of petroleum refining mud water, a binder of casting type, a mixing agent of ceramic raw material, and a base material of ointment. The name comes from the stratum of the Wyoming State, a discovery site.

The diatomaceous earth referred to in the present invention is mainly composed of silicic acid (SiO ₂ ) and is white or grayish white. It is light and soft enough to touch the powder with the finger. Since it is a minute porous material, it is highly absorbent and is a poor conductor of heat. The diatomaceous earth in our country is produced in soil form in the third or fourth strata. During the third phase of Koze III (Korea), fault movements in the direction of Northeast to North-North East occurred and several sedimentary basins were formed. Yeongil Basin, Gilju and Myeongcheon Gap, Gap between Seoul and Wonsan, and Tumen River basin were formed, and the third basement was deposited here. When deposits were formed, diatomaceous deposits were formed in small shallow lakes or freshwater basins, forming deposits. Among these sedimentary basins, diatomite in Yeongil Bay is the largest in diatomite deposits, and is concentrated in Wolsong and Yeongil in Gyeongsangbuk-do. In North Korea, there are also other areas in North Korea such as Hwanghyeon, Munsan-myeon, Ansan-myeon, Hamgyung-myeon, Anbyeon, Hamgyungbuk-do, Gyeongseong-gun, Nambyeon, Bupyeong-dong, Pyeonggang County, Cheonggyeongsaemyeo and is known as the origin of the cell. The development of diatomite as an ore in our country began in 1941 when it was incorporated into designated mining minerals by the Korean Mining Ordinance. Since then, domestic diatomaceous earth deposits have been found in various parts of the country. In particular, diatomaceous earth, which was produced in Yilli in Yangbuk-myeon, Wolsong-gun, Gyeongsangbuk-do, was used as a raw material and was used extensively in the production of Asahi Furnace in Japan. Recently, it has been used as a refractory material (refractory material) and because of its strong absorbency, it is mostly used as a filtering material for purification of industrial water, water, medicine, foods and the like. It is also used as a filler for plastics and paper, as an adsorbent for dynamite, as a hemostatic agent, as a mixture of cement.

In the present invention, germanium has an elemental symbol Ge, atomic number 32, and atomic weight of 72.59 ± 3. In 1871 D. I. Mendeleev discovered its existence and was called eicosil, but in 86 CE was discovered in Germany by C. A. Binkler, a German chemist named "Germania", which was discovered in Germanium (Ag GeS). Since it is similar to silicon, it is widely replaced by silicon of silicate in the crust. There are very few minerals mainly containing germanium although it is contained in sulfide minerals or coal containing copper or zinc.

The elvan stone referred to in the present invention is also referred to as a lavender. The chemical composition is KAl ₃ (SO ₄ ) ₂ (OH) ₆ . It appears as a hexagonal plate or scaly crystal, usually in a massive or granular form. A hardness of 3.5 to 4, and a specific gravity of 2.6 to 2.9. The crystal type has a complete cleavage on the underside, and the cleavage surface has a pearly luster. Massive ones are not glossy. It is white, gray and occasionally red, yellow, and reddish brown. The streak color is white. It does not dissolve in hydrochloric acid but dissolves in potassium hydroxide or hot sulfuric acid. It is produced in the volcanic rock area subjected to hydrothermal alteration.

In the present invention, oxiran means dense and hard, transparent, beautifully shined, polished and glossy. Mineralogically, nephrite is a kind of amphibole, and jadeite is a type of alkali pyroxene. Nephrite are many milky white, green, yellow, red, and jadeite are green and white. There are various names depending on the color, but Baekok and Jade are representative. Since ancient times, it has been regarded as a precious stone in the Orient, and has been used as an ornamental stone and jade pot. Nephrite is produced as a vein in crystalline schist or gneiss in Turkey and in the contact zone of granite in northeast China. In Myanmar, jadeite is produced in the form of a veil of serpentinite, and is also produced in Yunnan or Tibetan plateau in China. The narrow meaning jade refers to jade, but the present invention is not limited thereto, and the jade in the present invention is defined to include all of the jade in the above-mentioned broad sense.

The inorganic powder may have an average particle diameter of 0.005 to 30 탆.

Generally, since the inorganic powder is usually included in order to smooth the surface of various products, i.e., to serve as an abrasive, the average particle size of the inorganic powder is larger than that of the inorganic powder used in the present invention. However, in the present invention, the inorganic powder is intended to improve the growth performance by controlling the water quality and providing an effective ingredient for enhancing the growth activity of mineral and freshwater eel to freshwater eel. Especially to increase the survival rate by increasing immunity and increasing resistance to disease.

The inorganic powder may have an average particle diameter of 0.005 to 30 탆.

When the average particle diameter of the inorganic powder is less than 0.005 占 퐉, the inorganic powders are irregularly aggregated again, so that the dispersing ability is lowered and the cleaning effect due to the interaction of the inorganic sites is lowered. On the other hand, when the average particle size of the inorganic powder is more than 30 μm, since the size of the particles is relatively large, the inorganic powder may sink into the water tank to deteriorate the cleaning effect, and the water is not effectively absorbed into freshwater eel.

Preferably, the inorganic powder has an average particle diameter of 0.01 to 5 占 퐉. By the above range, the dispersibility is excellent, the effect as a carrier of the active ingredient is enhanced, the cleaning effect of the inorganic powder is improved, etc., and it is absorbed more smoothly in freshwater eel to enhance mineral supplementation and immunity enhancement effect Help can be beneficial.

Preferably the inorganic powder is the strontium ion (Sr ^{2 +),} calcium ions (Ca ^{2 +),} magnesium ions (Mg ^{2 +)} is, and the solution was mixed with heat-treated contains a mixture thereof at least one selected from the group consisting of the Lt; / RTI >

When the inorganic powder is mixed with the ion-containing solution and is heat-treated, not only the washing power of the inorganic powder itself increases, but also the inorganic powder is absorbed into the freshwater eel so that the ion and other actions greatly increase the survival rate against freshwater eel .

More preferably, the inorganic powder may be heat-treated by mixing with a solution containing strontium ions (Sr ^{2 +} ) and calcium ions (Ca ^{2 +} ). By the above range, it is possible to enhance the antimicrobial activity and increase the immunity activity against freshwater eel to increase the resistance to the diseases of freshwater eel and increase the survival rate and growth.

More preferably, the inorganic powder includes fine powder zeolite, wherein 20 to 60 parts by weight of a fine powder of shungite, 20 to 60 parts by weight of fine powder, 20 to 60 parts by weight of fine powder bentonite are mixed with 100 parts by weight of the zeolite, 50 to 100 parts by weight of a fine powdery diatomaceous earth and 10 to 30 parts by weight of a fine powdered germanium and mixed with a solution containing strontium ions (Sr ²⁺ ) and calcium ions (Ca ²⁺ ).

According to the above-mentioned range, the interaction of the powders constituting the inorganic powder enables the water quality to be suitable for survival of freshwater eel such as washing effect by more than a simple combination. In addition, it provides a mineral supply to freshwater eel, It can directly contribute to the survival rate of the eel by acting directly in the body.

The feed additive for freshwater eel culturing may further comprise a first plant extract selected from the group consisting of apricot tree extract, plum tree extract, cotton extract, and dandelion extract.

Preferably, the first plant extract comprises 30 to 80 parts by weight of a plum tree, 30 to 80 parts by weight of a cottonwood extract, and 1 to 5 parts by weight of a dandelion extract, based on 100 parts by weight of the apricot extract, .

By the above-mentioned range, the extract exhibits a higher antibacterial activity than the simple mixture due to the interaction of the extracts, and enhances the immunity against freshwater eel to enhance the survival rate against freshwater eel and enhance the growth.

In the feed additive for freshwater eel culturing, the first plant extract may be adsorbed on the inorganic powder. In particular, when the inorganic powder is impregnated with the first plant extract, it has an advantage that the persistence of the antibacterial effect and the immunity enhancement effect of freshwater eel according to the use of the first plant extract is enhanced.

The feed additive for freshwater eel culturing may further comprise a second plant extract selected from the group consisting of acorn extract, mandarin orange extract, and mixtures thereof.

Trapa japonica in the present invention grows in a pond or a marsh. The roots are embedded in the mud and the stem grows long and floats on the water. Leaves appear to be united and thick on the petiole, allowing it to float on the water as an air pocket. The leaf is triangular with a rhomboidal shape and has serrate. Feathery roots fall from the nodes of the main stream in the water. In July or August, white or slightly reddish flower blooms on the axil, with a diameter of about 1cm and four petals. The figurines are short and upward, but they bend downward as the fruit grows and are 2 to 4 cm long. Calyx leaf has hair, 4 stamens and 1 pistil. The fruit is rigid and inverted triangular with a thorn with calyx pieces on both ends and a central part being prominent.

The stiffness referred to in the present invention is also referred to as Kanghor. It grows in valley valley. It stands straight up to about 2m in height and branches at the top. Leaves are alternate phyllotaxis with petiole, and three small leaves are split into two flags. The small leaf is broad oval or ovate with pointed end and deep sawtooth on the edge. The petiole is shortened as it goes up, and the lower part of the petiole becomes broadened and becomes a sheath. In August or September, a white flower blooms on the end of a branch and at the end of a main stem in a double-layered inflorescence. It is divided into 10 to 30 small peduncles and has many flowers. There are one or two gun guns and six gun guns. Fruits are ripened in October and are elliptical with wings. It is fragrant and eats young seeds as herbs.

The second plant extract further enhances the cleaning effect, prevents secondary pollution due to the use of the feed, acts on the body of freshwater eel to enhance the environmental resistance, thereby increasing the survival rate and enhancing the growth.

Preferably, the second plant extract comprises a dried extract, and 20 to 60 parts by weight of the extract may be added to 100 parts by weight of the dried extract. According to the above range, the survival rate of the freshwater eel is maximized due to the synergy caused by the interaction.

Preferably, the feed additive for freshwater eel cultivation has an effect on prevention and improvement of tofu ulcer disease (atypical Aeromonas disease) of eel. The ulcer of the eel infected with the tofu ulcer disease of the eel occurs only in the head and not in the other part. The whiteness is visible in the vicinity of the snout and on the epidermis near the eyeball, and then the site swells up to a circular shape with a flare. As the disease progresses, the bulged part collapses to become an ulcer. In severe cases, the muscles of the affected part are melted and the skull is exposed.

When the feed additive for freshwater eel culture is used, the effect of supplementing minerals and enhancing immunity activity can be prevented and improved to prevent tofu ulcer disease of eel.

The feed for freshwater eel according to another embodiment of the present invention includes the feed additive for the freshwater eel culture.

The feed for the freshwater eel may be a mixture of a carbohydrate raw material, a protein raw material, and a vitamin raw material, and the specific range thereof includes all raw materials and compounding ratios that can be used by those skilled in the art.

According to another embodiment of the present invention, there is provided a method for preparing a feed additive for freshwater eel culturing, comprising the steps of: preparing a feed additive for freshwater eel culturing, A dispersion-mixing step of preparing a water-dispersed inorganic material for mixing and dispersing an inorganic powder selected from the group consisting of fine powder, jute and mixture thereof in purified water; And a second plant extract mixing step of mixing the water-dispersed inorganic material with the second plant extract selected from the group consisting of a flower extract, a gangrene extract, and a mixture thereof after the dispersion and mixing step.

The method for preparing the feed additive for freshwater eel culturing may further include a heat treatment step of applying heat treatment to the water-dispersed inorganic material after the dispersion and mixing step.

Preferably, the heat treatment step includes the inorganic powder is the strontium ion (Sr ^{+ 2),} calcium ion (Ca ^2+), magnesium ion (Mg ^{+ 2)} and the at least one selected from the group consisting of a mixture solution And may be heat-treated.

More preferably, the ionization step may be a solution containing strontium ions (Sr ^{2 +} ) and calcium ions (Ca ^{2 +} ). By the above range, the survival rate of freshwater eel can be greatly increased.

The method for preparing a feed additive for freshwater eel culture includes a first plant which mixes the first plant extract selected from the group consisting of apricot tree extract, plum tree extract, cotton extract and dandelion extract into the inorganic powder dispersion after the ion treatment step Extract mixing step.

That is, the method for producing the feed additive for freshwater eel cultivation is a method of producing a feed additive for freshwater eel culturing, which comprises the steps of: preparing a feed additive for freshwater eel cultured by a method selected from the group consisting of a fine powder, a fine powder, a fine powder, a zeolite, a fine powder, An inorganic powder selected from the group consisting of an inorganic powder which is impregnated with a first plant extract selected from the group consisting of apricot tree extract, plum tree extract, cotton extract and dandelion extract to impregnate the inside of the inorganic powder with the first plant extract, Impregnation step; Drying the inorganic powder impregnated with the first plant extract according to the tilting step; A second mixing step of mixing and dispersing the inorganic powder dried in accordance with the drying step with purified water, and a second mixing step of mixing the inorganic powder and the purified water mixture after the dispersion mixing step with a second plant selected from the group consisting of a waxy extract, And mixing the extract.

By the above-mentioned range, it is possible to obtain effects such as water quality environment for enhancing survival rate of freshwater eel and immune enhancement activity in the body.

The feed additive for freshwater eel according to the present invention is based on an inorganic material and a natural product, and can improve the growth of eels without the problem of its use and long-term use.

In addition, the feed additive for freshwater eel according to the present invention can increase the survival rate by supplying minerals to the freshwater eel and enhancing the immunity, and can prevent and improve the tofu ulcer disease commonly caused in freshwater eel.

The present invention provides a method for preparing the feed additive for freshwater eel.

The present invention provides a feed comprising the feed additive for freshwater eel.

Fig. 1 is a photograph of a severe head ulcer disease in freshwater eel.
Figure 2 relates to the symptoms of severe head ulcerative disease according to the use of the control group of the present invention.
Figure 3 is for freshwater eel with improved severity of tofu disease caused by the use of the preparation of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

1. Manufacture of inorganic powder particle size

The following components were mixed in the same mass to obtain a mixture of (a) purified water and (b) a mixture of fine powder and grit, as shown in Table 1 below, (b) Dispersion was carried out for 30 minutes in water collected from a water tank in which freshwater eel was cultured by adjusting the average particle size of the inorganic powder, and dispersed strongly for 1 minute or more using a homogenizer.

Example Average particle size (μm) S1 0.005 S2 0.01 S3 1.23 S4 5.03 S5 9.41 S6 30.1 S7 51.7

2. Evaluation of water-dispersibility of inorganic powders

The water dispersibility of the inorganic powders prepared according to the following Table 1 was evaluated to evaluate the degree of persistence of the dispersibility. As a result, the dispersibility and the sustainability of the general purified water were evaluated as shown in Table 2, Dispersibility and persistence in water are shown in Table 3. The dispersibility was evaluated as an index of 1 to 10, and the higher the number is, the better the dispersibility and dispersing persistence.

Example Dispersibility and dispersion persistence S1 2 S2 3 S3 7 S4 8 S5 8 S6 3 S7 One

Example Dispersibility and dispersion persistence S1 3 S2 8 S3 9 S4 8 S5 4 S6 One S7 One

Referring to [Table 2] and [Table 3], it can be seen that the dispersibility is somewhat different from the experiment in purified water in an environment where freshwater eel is cultured. In the environment where freshwater eel is cultured, it can be seen that the dispersibility and the sustainability are the most excellent according to S2 to S4. Therefore, it has been confirmed that the inorganic powder contained in the feed of freshwater eel in the present invention can exhibit excellent dispersion and persistence as an aqueous dispersion agent when the average particle diameter is maintained at 0.01 to 5 탆.

And can be easily mixed by the particle size, and can exhibit uniform dispersion and dispersion retention for a considerable time. This can provide an advantageous environment for maintaining the cleaning effect and the light scattering effect according to the use of the inorganic powder.

3. Mixed  Preparation of Inorganic Powder Dispersion

In order to ascertain the synergistic effect of the mixture of the fine powder and the grit, the fine powder, the fine powder, the fine powder, the zeolite, the fine powder, the bentonite, the fine powder, Inorganic powder was prepared as shown in Table 4. On the other hand, the average particle size of the above-mentioned fine powders, fine powders, fine powder zeolites, fine powder compacts, fine powder bentonites, fine powdered diatomaceous earths, fine powdered germanium, fine powdered yellow loam, In addition, the inorganic powder was prepared by mixing a solution containing the strontium ion (Sr ^{2 +)} and calcium ions (Ca ^2+), and mixing the heat-treated o 'clock purified water for 1 hour. On the other hand, when the inorganic powder is not subjected to ion treatment so as to correspond to T1, it is preferable that the same weight of the fine powder, the fine powder, the fine powder, the fine powder zeolite, the fine powder, the fine powder bentonite, the fine powder diatomaceous earth, the fine powder germanium, To prepare TX.

T1 T2 T3 T4 T5 T6 T7 T8 T9 Zeolite 100 100 100 100 100 100 100 100 100 ? Gait 100 30 30 30 10 20 40 60 80 Elven stone 100 - 30 30 10 20 40 60 80 Bentonite 100 - - 30 10 20 40 60 80 Diatomaceous earth 100 - - - 30 50 75 100 125 germanium 100 - - - 5 10 20 30 40

(Unit: parts by weight)

4. Bacteria inhibitory activity test

In order to confirm the bacterial inhibitory activity of the above preparation example, a medium containing saline was prepared, and T1 to T9 were added thereto, followed by inoculation with Vibrio fluvialis, followed by incubation for 24 hours After the incubation, the effect on inhibition of growth of Vibrio fluvialis was measured. The inhibitory effect is shown in% compared to the control without addition of T1 to T9. The lower the value, the better the effect on the bactericidal activity.

The results of the cleaning power test and the conformity test are shown in Table 5 below.

T1 T2 T3 T4 T5 T6 T7 T8 T9 TX Bacterial inhibition 89 108 107 91 76 47 52 51 83 142

(Unit: exponent,%) Referring to Table 5 above, it can be seen that the effect of suppressing the growth of pathogenic bacteria is enhanced by the synergistic effect due to the interaction in the range from T6 to T8. On the other hand, the effect of inhibiting the activity of Vibrio fluvialis in T5 to T9 was confirmed. On the other hand, in the case of TX, the effect of inhibiting bacterial growth is relatively lowered. Therefore, it can be seen that the activity of inhibiting bacterial growth by the interaction of the inorganic flux and the ion treatment is increased.

Therefore, according to the present invention, it can be seen that the survival rate can be increased by enhancing the antibacterial and immunity of freshwater eel.

5. The first plant extract-adsorbed inorganic powder dispersion

A plant extract constituted as shown in the following [Table 6] was prepared, The inorganic powder prepared according to Preparation Example T7 was mixed with the plant extract to adsorb the plant extract to the inorganic powder.

On the other hand, each of the following apricot tree extract, plum tree extract, cottonwood extract and dandelion extract was prepared by mixing and extracting each apricot tree, plum, cottonwood and dandelion with hot water.

C1 C2 C3 C4 C5 C6 C7 C8 C9 Apricot Tree Extract 100 100 100 100 100 100 100 100 100 Plum tree extract - 30 - - 20 30 50 80 100 Cottonwood extract - - 30 - 20 30 50 80 100 Dandelion extract - - - 30 0.5 One 3 5 7

(Unit: parts by weight)

6. A composition comprising a first plant extract Bacteria inhibitory activity against inorganic powders  Experiment

The above C1 to C9 plant extracts were adsorbed on T7 and dispersed in purified water. Each mixture was injected into each medium, and the effect of suppressing the growth of Vibrio fluvialis was confirmed by the same method as the above-mentioned bacteriostatic activity test. The results are shown in Table 7 below.

C1 C2 C3 C4 C5 C6 C7 C8 C9 Bacterial inhibition 67 62 52 56 58 41 48 51 66

(Unit: index,%)

Referring to Table 6, it can be seen that the activity to inhibit bacterial growth is increased according to the plant extracts impregnated with C6 to C8. Therefore, when the plant extracts are mixed and used, a synergistic effect on the bacterial growth inhibitory activity of the inorganic flux treated in the ionic solution appears.

Especially, it was confirmed that the synergistic effects of the plant extracts from C6 to C8 are exhibited by the interaction.

7. Preparation of 2nd Plant Extract

The plant extract C7 was adsorbed on the inorganic powder T7, dispersed in water, and mixed with a second plant extract having the composition shown in Table 8 below. The following acorn extracts and ganghyeol extracts were prepared by hot water extraction and filtration as in the case of the first plant extract.

D1 D2 D3 D4 D5 D6 Acorn extract 100 100 100 100 100 - Ganghyeong extract - 10 20 60 100 100

(Unit: parts by weight)

8. A composition comprising a second plant extract Suppression of bacterial growth on inorganic powders  Active experiment

C7-adsorbed T7 was mixed with the plant extracts of D1 to D6, and the effect of inhibiting the growth of Vibrio fluvialis was confirmed by the same method as the above-mentioned bacteriostatic activity test. The results are shown in Table 9 below.

D1 D2 D3 D4 D5 D6 Antimicrobial 45 39 32 21 38 44

(Unit: index,%)

Referring to Table 9, it can be seen that the activity to inhibit bacterial proliferation increases when the second plant extract is included. In particular, the inhibitory activity of bacterial proliferation is greatly increased by D3 and D4. It can be seen that the activity of inhibiting the proliferation of bacteria is enhanced by the synergistic effect by interaction in the combination range of D3 and D4.

Therefore, when the composition containing the inorganic powder is included in the feed and used continuously, the effect of inhibiting the growth of bacteria in an environment where the eel is cultured can be obtained.

9. Freshwater eel  Severe Tofu in the disease  Experiment on improvement effect

T4, T7, T9, C4, C6, C9, D2, D4, D5 as shown in the following Table 10 to confirm the improvement effect of the feed additive for freshwater eel culture according to the present invention against freshwater eel- Were mixed with 2w% of the total weight of the feed to form a sludge-type feed based on the egg yolk ingredient of the fish eggs and mixed with other protein, carbohydrate and vitamins-containing freshwater eels to obtain FM1 to FM10 . On the other hand, as a control group, a general diet used for freshwater eel culture without any treatment was provided.

As shown in FIG. 2, 10 eels with severe head ulcer disease were classified into breeding tanks, and the feeds were classified into a control group and FM1 to FM10 while being aerated in an exponential manner for 7 days. And the same conditions were maintained, except for the above. The results are shown in Table 11 below.

Control FM1 FM2 FM3 FM4 FM5 FM6 FM7 FM8 FM9 FM10 additive - T1 T4 T7 T9 C4 C6 C9 D2 D4 D5

Days of Breeding Control FM1 FM2 FM3 FM4 FM5 FM6 FM7 FM8 FM9 FM10 One 0 0 0 0 0 0 0 0 0 0 0 7 0 10 20 40 20 40 60 30 70 90 60

(Treated individual, unit:%)

Referring to the above Table 11, it can be seen that FM1 and FM2 can exhibit some improvement of severe tofu ulcer disease in freshwater eel. In particular, FM3 shows a remarkably high improvement effect. This is interpreted as a result of a synergistic effect due to the interaction of each inorganic powder with the use of the above-mentioned C7 to enhance proper mineral supply, antibacterial activity and immunity. Therefore, it has been confirmed that the present invention can prevent and improve tofu disease of freshwater eel by the range of mixing. On the other hand, in the case of FM5, 7, 8 and 10, the degree of survival improvement was not significantly higher than that of FM3. However, it can be seen that the effect of FM6 and FM9 on tofu ulcer disease is significantly improved. This is because when the first and second plant extracts are mixed and used in a certain range, the antimicrobial effect and the effect of improving the antibacterial effect and the immune activity against the freshwater eel are improved and the survival rate of the tofu of the freshwater eel is maintained can confirm.

Therefore, it can be seen that the present invention can increase the production through prevention and improvement of tofu disease, which is common in freshwater eel culture.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, Of the right.

Claims (8)

20 to 60 parts by weight of fine powder, 20 to 60 parts by weight of fine powder bentonite, 20 to 60 parts by weight of fine powdered bentonite, 50 to 100 parts by weight of fine powdered zeolite, And 10 to 30 parts by weight of fine germanium germanium,
The inorganic powder is heat-treated by mixing with a solution containing strontium ions (Sr ²⁺ ) and calcium ions (Ca ²⁺ )
A first extract comprising 30 to 80 parts by weight of a plum tree, 30 to 80 parts by weight of a cottonwood extract, and 1 to 5 parts by weight of a dandelion extract, based on 100 parts by weight of the apricot tree extract;
And a second plant extract containing 20 to 60 parts by weight of a gangbyeol extract, based on 100 parts by weight of the gangbang extract
Feed additives for freshwater eel farming. The method according to claim 1,
The inorganic powder has an average particle diameter of 0.005 to 30 mu m
Feed additives for freshwater eel farming. delete delete A feed additive for freshwater eel according to claim 1 or 2,
Feed for freshwater eel farming. 20 to 60 parts by weight of fine powder, 20 to 60 parts by weight of fine powder bentonite, 20 to 60 parts by weight of fine powdered bentonite, 50 to 100 parts by weight of fine powdered zeolite, An ion treatment step of mixing and dispersing an inorganic powder containing 10 to 30 parts by weight of germanium fine powder into a solution containing strontium ions (Sr ²⁺ ) and calcium ions (Ca ²⁺ );
A heat treatment step of applying heat treatment to the dispersion of the inorganic material that has undergone the ion treatment step after the dispersion and mixing step;
A first plant extract containing 30 to 80 parts by weight of a plum tree, 30 to 80 parts by weight of a cottonwood extract, and 1 to 5 parts by weight of a dandelion extract is added to 100 parts by weight of the apricot tree extract, Mixing step and
And a second plant extract mixing step of mixing the second plant extract containing 20 to 60 parts by weight of the extract of Ganoderma lucidum with 100 parts by weight of the ganoderma lucidum extract,
A method for preparing a feed additive for freshwater eel farming. delete delete

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