KR102142887B1 - Feed additive composition for macrobrachium rosenbergii - Google Patents

Abstract

The present invention relates to a feed additive composition for shrimp. Feed additives for shrimp according to an embodiment of the present invention are fine powder š-gite (shungite), fine powder illite (illite), fine powder zeolite (zeolite), fine powder elvan stone, fine powder bentonite, fine powder diatomaceous earth, fine powder germanium, fine powder loess, fine powder bag It contains 0.01 to 100 parts by weight of an additive including an inorganic powder selected from the group consisting of rock, fine powder jade, and mixtures thereof.
An object of the present invention relates to a feed additive for shrimp that is based on inorganic and natural substances, and can increase the growth of shrimp without problems due to its use and long-term use.

Description

Feed additive composition for shrimp {FEED ADDITIVE COMPOSITION FOR MACROBRACHIUM ROSENBERGII}

The present invention relates to a feed additive composition for shrimp. In more detail, the present invention relates to a feed additive composition for shrimp that includes minerals mixed in a certain range and increases the immune activity and environmental resistance of shrimp to improve the survival rate and growth rate.

The fish farming industry in recent years faces a decrease in the activity of fish due to the increased stress caused by the deterioration of the breeding environment and the pollution of the water quality environment, and the decline in growth, the loss of meat quality, and the misuse of antibiotics. In particular, since the accumulation of fish due to the misuse of antibiotics, the spread of antibiotic-resistant strains, and water pollution are a series of problems, the development of new alternative additives for enhancing natural immunity and disease prevention using natural raw materials is essential at this time. .

Aquatic foods such as shrimp, a representative crustacean cultured fish species, are increasing in quality and quantity day by day due to increased national income and interest in health. Various types of shrimp (Penaeus), including white-legged shrimp belonging to the Shrimp family, are widely distributed over 2,500 species in all parts of the world including the Pacific coast and Asia. Most of them live in seawater and contain a lot of chitosan and calcium taurine. The aquaculture area is steadily increasing. Nevertheless, it suffers a lot of difficulties due to mass mortality and productivity decline due to disease infection and destabilization of water quality at the larval stage.

Specifically, feeds used in shrimp farming include raw feeds such as komji (small shrimps) or clams, frozen feeds from frozen catches, and blended feeds supplemented with various vitamins and minerals based on fishmeal.

Among them, compounded feed is usually manufactured using a pellet machine. Due to the nature of shrimps, they do not eat food at once, but because they have the habit of squatting the feed with a bokjeolji (the leg in the stomach) and eating it with the mouth, the feed is underwater. If it collapses easily, it cannot be eaten. It accumulates and decays on the farm floor, polluting the water quality, slowing the growth of shrimp and causing disease.

Therefore, in order to improve the water stability of the pellet feed, feed is prepared using a triple conditioner and a post-conditioner.

In addition, artificial binders that can increase the binding power of feed were also added to the feed composition. However, the artificial binding agent as described above was the cause of raising the feed cost, and the use of a large amount of expensive fish meal is also considered as one of the causes of raising the feed cost.

On the other hand, shrimp farming is being farmed in festival-style farms in coastal or estuary areas, and they are seriously affected by viral diseases caused by the inflow of seawater. In Korea, there is an increasing number of onshore shrimp farming facilities that can prevent the influx of viruses, but in the case of current farms, food waste or excrement accumulates due to continuous feed supply, resulting in an odor due to deterioration of water quality. Although the major causes of ammonia, nitrite, and nitrate are increased, which has a fatal effect on the growth of shrimp, a large amount of mortality has occurred. However, the development of feed additives for shrimp farming has not been made at all.

In view of these points, it is very necessary to develop aquaculture feed for shrimp that can prevent diseases while having better feed efficiency than high functional feed additives or imported feed.

KR 10-2012-0081841 A KR 10-2012-0044804 A

An object of the present invention relates to a feed additive for shrimp that is based on inorganic and natural substances, and can increase the growth of shrimp, without any problems due to its use and long-term use.

In addition, the present invention is to provide a feed additive for shrimp that increases the survival rate of shrimp farmed by supplying minerals and enhancing immunity, and increases the survival rate of farmed shrimp by increasing resistance to various diseases, thereby improving economic efficiency.

The present invention provides a method for producing the feed additive for shrimp.

In order to achieve the above object, the feed additive for shrimp according to an embodiment of the present invention is fine powder š-gite (shungite), fine powder illite (illite), fine powder zeolite (zeolite), fine powder elvan stone, fine powder bentonite, fine powder diatomaceous earth, It contains 0.01 to 100 parts by weight of an additive including an inorganic powder selected from the group consisting of fine powder germanium, fine powder loess, fine powder alum, fine powder jade, and mixtures thereof.

The inorganic powder may have an average particle diameter of 0.005 to 30 μm.

The feed additive for shrimp may further include a first plant extract selected from the group consisting of apricot tree extract, plum tree extract, cotton extract, and dandelion extract.

The feed additive for shrimp may further include a second plant extract selected from the group consisting of drywall extract, ganghal extract, and mixtures thereof.

The shrimp may be a king spider shrimp (Macrobrachium rosenbergii).

The method of manufacturing a feed additive for shrimp according to another embodiment of the present invention is a fine powder š-gite, fine powder illite, fine powder zeolite, fine powder elvan stone, fine powder bentonite, fine powder diatomaceous earth, fine powder germanium, fine powder loess, fine powder alum, fine powder jade And a dispersion mixing step of preparing a water-dispersible inorganic material for mixing and dispersing the inorganic powder selected from the group consisting of a mixture thereof in purified water. After the dispersion mixing step, it may include a second plant extract mixing step of mixing a second plant extract selected from the group consisting of a dry matter extract, a ganghal extract, and a mixture thereof with the water-dispersible inorganic material.

The method of manufacturing the feed additive for shrimp may further include a heat treatment step of applying heat treatment to the water-dispersed inorganic material after the dispersion mixing step.

The method for preparing the feed additive for shrimp is a first plant extract mixing of a first plant extract selected from the group consisting of apricot tree extract, plum tree extract, cotton extract, and dandelion extract in the inorganic powder dispersion after the ion treatment step. It may include a step.

Hereinafter, the present invention will be described in more detail.

Feed additives for shrimp according to an embodiment of the present invention are fine powder š-gite (shungite), fine powder illite (illite), fine powder zeolite (zeolite), fine powder elvan stone, fine powder bentonite, fine powder diatomaceous earth, fine powder germanium, fine powder loess, fine powder It includes 0.01 to 100 parts by weight of an additive including an inorganic powder selected from the group consisting of alum stone, fine powder jade, and mixtures thereof.

The fine powder referred to in the present invention refers to crushing by a ball mill, jet mill, or other pulverizing device, or manufacturing to have the properties of a fine powder during the synthesis process, and the fine powder is defined as having an average particle diameter of 100 μm or less.

In the present invention, the šœgaitran, in Shunga near Suojδrvi in the northern part of the Karelia Ladoga in Russia, is a layer (up to 2m thick) or a lenticular body among shale rich in small graphite microcrystals from the Jatulian era of the Protozoic era. Is calculated. It is black like pitch, dense, opaque, metallic luster. It is soft. Hardness 2.5. d 1.75~1.80, making graphite acid from concentrated nitric acid without melting in a fire zone.

Illite in the present invention has a chemical composition of (K,H ₃ O)Al ₂ (Si,Al) ₄ O ₁₀ (H ₂ O,OH) ₂ . In terms of chemical composition, some are close to muscovite mica, but relatively SiO ₂ , MgO, H ₂ O are high and K ₂ O is low. It is a mineral of the same family as hundreds of mica. Hardness 1 to 2, specific gravity 2.6 to 2.9. The cleavage is complete with [001], the streak color is white, and there is a earthy gloss. There is an opinion that it is not an independent mineral in terms of its chemical composition or crystal structure, but a mixed mineral containing other components. It is produced in aluminum-rich heterogeneous or tuff-like sedimentary rocks, and is produced as a metamorphic mineral of the hydrothermal mineral bedrock.

Zeolite referred to in the present invention is also referred to as a tombstone (沸石). There are many types, but they have a lot of water content, properties of crystals, and the like. Hardness does not exceed 6, and specific gravity is about 2.2. It is generally colorless, transparent or white translucent. When heated with a blowpipe, it boils and becomes bulky, so it got its name. Most types dissolve in hydrochloric acid and are often glued, but few types do not dissolve in hydrochloric acid. As the main types, there are Bangmonite, Fisheye, Chabazite, Soda Stone, Heullandite, Steelbite, Lomontite, and Innessite. It is produced from voids or fissures of basic igneous rocks such as basalt or tuff, and sometimes exists as a secondary mineral among granite and gneiss. In addition, it may be produced in gold or other veins. In the crystal structure, the bonds of each atom are loose, and even if the moisture filling between them is released by high heat, the skeleton remains intact, so other particulate matter can be adsorbed. Using this property, it is used as an adsorbent, and it is used as a molecular sieve that separates fine particles of different sizes.

Elvan stone referred to in the present invention is known as a term used in Chinese herbal medicine, and it belongs to granite class geologically. It is the same as quartz porphyry, feldspar porphyry, and granite porphyry, and the name of the rock coincides with quartz-monzonite. Quartz and feldspar are densely mixed. Yellowish white, light yellowish brown, light gray, dark green or light green rocks with red dots or white dots evenly mixed together are like rice balls made of barley, so the name Elvan Stone was given. The main components are silicic anhydride and aluminum oxide, and it contains a small amount of ferric oxide. Known as the yellowish white elvan stone, it was used as an anti-inflammatory agent to treat skin diseases such as swelling or boils on the back and a filter to purify pills. According to Donguibogam, its nature is sweet, warm, and non-poisonous. It consists of 30,000 to 150,000 holes per 1 cm ^3, so it has strong adsorption and contains about 25,000 kinds of inorganic salts. It is also used as a toxic metal removal agent because it exchanges ions with heavy metals, and it is known to emit far-infrared rays when heat is applied to this rock. Because of this characteristic, it is used in various industrial parts such as jjimjilbang, tableware, and medical equipment.

Benzoite used in the present invention contains quartz, feldspar, zeolite, and the like. The color is white, gray, pale brown, pale green. It has pearl gloss and lead gloss, and is produced as a dense mass with a sense of fat. It is similar to the properties of montmorillonite, such as swelling by adsorbing water and having clear cation exchange. Tuff and vitreous rhyolite are deteriorated. Its use is very wide, and it is also used as the main component of Isu for petroleum refinery, as a binder for castings, as a mixing agent for ceramic raw materials, and as a base for ointments. The name comes from the strata of Wyoming, the United States, where it was discovered.

Diatomaceous earth referred to in the present invention is mainly made of silicic acid (SiO ₂ ), and has a white or grayish white color. It is light and soft enough to smear powder when touched with your fingers. Because it is a fine porous material, it has strong absorbency and is a poor conductor of heat. Diatomaceous earth in our country is calculated as soil in the tertiary or fourth stratum. During the third period of the ancient period of our country, fault movements in the northeast to northeast directions occurred, forming sedimentary basins in several places. The Yeongil Basin, the Earth Zone between Gilju and Myeongcheon, the Earth Zone between Seoul and Wonsan, and the Tuman River Basin were formed, and the third base was deposited there. When sedimentary layers were formed, diatoms were deposited in small shallow lakes or freshwater basins in various places to form deposits. Among these sedimentary basins, diatomaceous earth in Yeongil Bay is the largest in diatomite deposits, and is found intensively in Wolseong and Yeongil, Gyeongsangbuk-do. In addition, in North Korea, Haenghyeon-ri, Munsan-myeon, Anbyeon-gun, Anbyeon-gun, Namsan-ri, Anbyeon-myeon, Gyeongseong-gun, Hamgyeongbuk-do, Hoemun mine in Bupyeong-dong and Jubuk-myeon, Gyeongseong-gun, Sanbon mine in Ungpyeong-myeon, Gilju-gun, Myeongcheon mine in Myeongcheon-gun, Baekrok mine in Seomyeon, Myeongcheon-gun, Gangwon-do The production centers of Sephori, Gosaph-myeon, Pyeonggang-gun are known. The development of diatomaceous earth as a deposit in Korea began in 1941 when it was incorporated as a designated legal mineral by the'Chosun Mining Ordinance'. Since then, domestic diatomite deposits have been discovered in various places, and in particular, diatomaceous earth produced in Iil-ri, Yangbuk-myeon, Wolseong-gun, Gyeongsangbuk-do, is said to have been used as a raw material for the manufacture of Asahi furnaces in Japan at Nodong-ri, Gyeongju. In recent years, it is used as a refractory material, and because of its strong absorption, it is mostly used as a filter material for purification of industrial water, water, medicine, and food. In addition, it is used as a filler for plastics and paper, adsorbent for dynamite, hemostatic agent, and a mixture of cement.

Germanium in the present invention has an element symbol Ge, an atomic number of 32, and an atomic weight of 72.59±3. Its existence was predicted by D. I. Mendeleev in 1871, and it was called Ekasil. However, it was discovered in Ag GeS by German chemist C. A. Binkler in 86 and named after German Latin name ``Germania''. Since it is similar to silicon, it is widely present by substituting with silicon in the silicate in the crust. Also, it is contained in sulfide minerals or coals containing copper or zinc, but there are very few minerals based on germanium.

Elvan stone referred to in the present invention is also referred to as alum stone. The chemical composition is KAl ₃ (SO ₄ ) ₂ (OH) ₆ . It appears as a crystal of a hexagonal plate (板狀) or impression (鱗狀), and is usually a mass or granular (粒狀). Hardness 3.5 to 4, specific gravity 2.6 to 2.9. Crystalline ones have a complete split on the bottom and a pearly luster on the split side. The odd one is not glossy. It is white, gray and sometimes red, yellow, or reddish brown. Streak color (條痕色) is white. Insoluble in hydrochloric acid, soluble in potassium hydroxide or hot sulfuric acid. It is produced in the volcanic rock area that has undergone a change of hydrothermal water.

The term jade in the present invention refers to dense, hard, transparent, beautifully shiny, polished and glossy. Mineralogically, purgatory is a type of amphibole, and jadeite is a type of alkali pyrite. Purgatory is often milky white, and there are also green, yellow, and red, and jadeite is green and white. There are various names depending on the color, but white jade and jade are typical. Since ancient times, it has been prized in the East, and has been used as a decorative stone and jadeite by handwork. Purgatory is produced as veins in crystalline schist or gneiss in Turkey and elsewhere, and in the northeast of China in the contact zone of granite (接觸帶). Jadeite is produced by forming veins among serpentine in Myanmar, and is also produced in Yunnan, China and Tibetan Highlands. Although jade in the narrow sense refers to jade, the present invention is not limited thereto, and jade in the present invention is defined as including all of the jade in the broad sense described above.

The inorganic powder may have an average particle diameter of 0.005 to 30 μm.

In the case of the inorganic powder having an average particle diameter of less than 0.005 μm, since the inorganic powder is again irregularly aggregated, the dispersing power is lowered, and the cleaning effect due to the interaction of each inorganic particle is deteriorated. On the other hand, when the average particle diameter of the inorganic powder exceeds 30 μm, the inorganic powder is settled in the water tank because the particle size is relatively large, and the washing effect is deteriorated, and there is a problem that it is not effectively absorbed by the shrimp.

Preferably, the inorganic powder may have an average particle diameter of 0.01 to 5 μm. In the case of the above range, it is excellent in dispersibility, the effect as a carrier of the active ingredient is enhanced, as well as the washing effect of the inorganic powder, and it is absorbed more smoothly by the shrimp, helping to replenish minerals and enhance immunity. This can be advantageous.

Preferably, the inorganic powder is heat treated by mixing with a solution containing any one selected from the group consisting of strontium ions (Sr ^{2 +} ), calcium ions (Ca ^{2 +} ), and magnesium ions (Mg ^{2 +} ) and mixtures thereof. Can be.

When the inorganic powder is mixed with the solution containing the ions and subjected to heat treatment, not only the cleaning power of the inorganic powder is increased, but also the survival rate for the shrimp can be greatly increased due to the ions and other actions as the inorganic powder is absorbed by the shrimp. .

More preferably, the inorganic powder may be heat-treated by mixing with a solution containing strontium ions (Sr ^{2 +} ) and calcium ions (Ca ^{2 +} ). In the case of the above range, it is possible to enhance the antibacterial power and increase the resistance to diseases of the shrimp by increasing the immune activity to the shrimp, and increase the survival rate and growth property.

More preferably, the inorganic powder includes fine powder zeolite, and 20 to 60 parts by weight of fine powder shungite, 20 to 60 parts by weight of fine powder elvan stone, 20 to 60 parts by weight of fine powder bentonite based on 100 parts by weight of the zeolite Part, 50 to 100 parts by weight of fine powdered diatomaceous earth, 10 to 30 parts by weight of fine powdered germanium are included, and may be heat treated by mixing with a solution containing strontium ions (Sr ^{2 +} ) and calcium ions (Ca ^{2 +} ).

In the case of the above range, the interaction of the powders constituting the inorganic powder allows the shrimp to become a water quality suitable for survival, such as a cleaning effect more than a simple combination, as well as mineral supply and antibacterial activity to the shrimp in the body. It can directly act on and contribute to greatly increase its survival rate.

The feed additive for shrimp may further include a first plant extract selected from the group consisting of apricot tree extract, plum tree extract, cotton extract, and dandelion extract.

Preferably, the first plant extract contains an apricot tree extract, and 30 to 80 parts by weight of plum, 30 to 80 parts by weight of cotton tree extract, and 1 to 5 parts by weight of dandelion extract are mixed with respect to 100 parts by weight of the apricot tree extract. I can.

In the case of the above range, it exhibits higher antibacterial activity than simple mixing due to the interaction of each extract, and increases the survival rate of shrimp by increasing immunity to shrimp, thereby increasing the productivity of farmed shrimp as well as enhancing the growth of shrimp. It can produce the effect of letting go.

In the feed additive for shrimp, the first plant extract may be adsorbed to the inorganic powder. In particular, when the inorganic powder is impregnated with the first plant extract, it has the advantage of increasing the antibacterial properties and the persistence of the effect of increasing the immunity of shrimp according to the use of the first plant extract.

The feed additive for shrimp may further include a second plant extract selected from the group consisting of drywall extract, ganghal extract, and mixtures thereof.

Marum (Trapa japonica) in the present invention grows in ponds or swamps. The roots are buried in mud and the stems grow long and float on the water. The leaves look clumped and there is a thick part on the petiole, which is an air bag that allows it to float on the water. The leaf body is a triangle similar to a rhombus and has fine serrations. Feather-like roots fall from the nodes of the main stem in the water. White or somewhat reddish flowers bloom in the axils of leaves in July or August, with a diameter of about 1cm, with 4 petals. Flower pieces are short and face up, but as the fruit grows, it bends down and is 2 to 4cm long. Calyx leaves have hairs, stamens are 4, pistils are 1. The fruit is hard and inverted triangle, with thorns with changed calyx at both ends, and the central part is prominent.

Ganghwa in the present invention is also called Ganghori. It grows in valley valleys. The height is about 2m, it stands upright, and branches are cut from the top. Leaves are alternate and have petioles, and 3 small leaves are split into pinnates twice. The small leaf is wide oval or egg-shaped, with a pointed end and deep serrated teeth at the edge. The small petiole gets shorter as it goes up, and the bottom of the petiole becomes wider to become a sheath. In August or September, white flowers bloom at the ends of branches and main stems in double mountain inflorescences, split into 10 to 30 small flowers, and many flowers hang. There are 1 or 2 guns, and there are 6 small guns. The fruit is a branch, ripens in October, is oval and has wings. It smells and eats young sprouts as herbs.

The second plant extract can further improve the cleaning effect, prevent secondary pollution caused by feed use, and increase environmental resistance by acting in the body of the shrimp, thereby increasing the survival rate and increasing the growth.

Preferably, the second plant extract may include a drywall extract, and may contain 20 to 60 parts by weight of a Kangwha extract based on 100 parts by weight of the drywall extract. In the case of the above range, the survival rate of shrimp is highest due to the synergistic effect due to the interaction.

The shrimp may be a king spider shrimp (Macrobrachium rosenbergii).

Giant spider shrimp refers to freshwater shrimp with a body length of 90mm for males and 70mm for females. The forehead horn is shallow and seems to face slightly downward, but the tip is slightly upward. The ridge leading to the forehead horn reaches near the middle of the carapace, and the upper edge of the forehead horn has 9 to 12 teeth-shaped processes, and the rear 3 to 4 are on the carapace. There are 1 to 4 teeth-shaped processes on the lower edge. The telson is shorter than the caudal leg, and there are 2 pairs of spines on the top and rear edge. Since the size of the squid shrimp is large and the taste is excellent, the preference is high. On the other hand, the growth property is very important because marketability can be recognized only when it is grown above a certain size.

In the case of the additive for feed for shrimp according to the present invention, it is excellently recognized that the effect of increasing the growth properties of the king spider shrimp is particularly excellent.

The method of manufacturing a feed additive for shrimp according to another embodiment of the present invention is a fine powder š-gite, fine powder illite, fine powder zeolite, fine powder elvan stone, fine powder bentonite, fine powder diatomaceous earth, fine powder germanium, fine powder loess, fine powder alum, fine powder jade And a dispersion mixing step of preparing a water-dispersible inorganic material for mixing and dispersing the inorganic powder selected from the group consisting of a mixture thereof in purified water. After the dispersion mixing step, it may include a second plant extract mixing step of mixing a second plant extract selected from the group consisting of a dry matter extract, a ganghal extract, and a mixture thereof with the water-dispersible inorganic material.

The method of manufacturing the feed additive for shrimp may further include a heat treatment step of applying heat treatment to the water-dispersed inorganic material after the dispersion mixing step.

Preferably, in the heat treatment step, the inorganic powder is a solution containing any one selected from the group consisting of strontium ions (Sr ^{2 +} ), calcium ions (Ca ²⁺ ), magnesium ions (Mg ^{2 +} ), and mixtures thereof. It may be mixed with and heat-treated.

More preferably, the ionization step may be performed by using a solution containing strontium ions (Sr ^{2 +} ) and calcium ions (Ca ^{2 +} ). In the case of the above range, the survival rate of shrimp can be greatly increased.

The method for preparing the feed additive for shrimp is a first plant extract mixing of a first plant extract selected from the group consisting of apricot tree extract, plum tree extract, cotton extract, and dandelion extract in the inorganic powder dispersion after the ion treatment step. It may include a step.

That is, the preparation method of the feed additive for shrimp is composed of fine powder š-gite, fine powder illite, fine powder zeolite, fine powder elvan stone, fine powder bentonite, fine powder diatomaceous earth, fine powder germanium, fine powder loess, fine powder alum, fine powder jade, and mixtures thereof. Inorganic powder impregnation in which the first plant extract is impregnated into the porous interior of the inorganic powder by impregnating the inorganic powder selected from the group into the first plant extract selected from the group consisting of apricot tree extract, plum tree extract, cotton extract, and dandelion extract. step; A drying step of drying the inorganic powder impregnated with the first plant extract according to the sedimentation step; A dispersion mixing step of mixing and dispersing the inorganic powder dried according to the drying step in purified water, and after the dispersion mixing step, a second plant selected from the group consisting of an inorganic powder and purified water mixture, a dry extract, an extract and a mixture thereof It may be to include the step of mixing the extract.

In the case of the above range, it is possible to play a large role in increasing the shipments of farmed shrimps by increasing the growth properties while producing effects such as water quality environment and immune enhancing activity in the body to increase the survival rate of shrimp.

The shrimp feed additive according to the present invention is based on inorganic and natural substances, and can increase the growth of shrimp without any problems due to its use and long-term use.

In addition, the feed additive for shrimp according to the present invention is provided to increase the survival rate of shrimp farmed by supplying minerals and enhancing immunity, and increasing the survival rate of farmed shrimp by increasing resistance to various diseases, thereby improving economic efficiency.

Another object of the present invention is to provide a method of manufacturing the feed additive for shrimp.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art to which the present invention pertains can easily practice. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein.

1. Preparation of inorganic powder particle size

As shown in [Table 1] below, fine powder gite, fine powder illite, fine powder zeolite, fine powder elvan stone, fine powder bentonite, fine powder diatomaceous earth, fine powder germanium, fine powder loess, fine powder alum stone, fine powder jade are mixed in the same mass. (a) Purified water And (b) Disperse for 30 minutes in water collected from the aquaculture tank, and after strongly dispersing for at least 1 minute using a homogenizer, the dispersibility was evaluated.

Example Average particle diameter (μm) S1 0.005 S2 0.01 S3 1.23 S4 5.03 S5 9.41 S6 30.1 S7 51.7

2. Evaluation of water dispersibility of inorganic powder

The degree of dispersibility was evaluated by evaluating the water dispersibility of the inorganic powder prepared according to the following [Table 1]. As a result, the dispersibility and persistence of general purified water were evaluated in [Table 2]. Dispersibility and persistence in water are shown in [Table 3]. The dispersibility was evaluated by an index of 1 to 10, and the higher the number, the better the dispersibility and dispersion persistence.

Example Dispersion and dispersion persistence S1 2 S2 3 S3 7 S4 8 S5 8 S6 3 S7 One

Example Dispersion and dispersion persistence S1 3 S2 8 S3 9 S4 8 S5 4 S6 One S7 One

Referring to [Table 2] and [Table 3] above, it can be seen that the dispersibility in the environment of the aquaculture is somewhat different from the result of the experiment in purified water. It can be seen that in the aquarium environment of the aquaculture, the dispersibility and the degree of persistence are the best in the case of S2 to S4. Therefore, it was confirmed that the inorganic powder contained in the shrimp feed in the present invention can exhibit excellent dispersion and persistence as an aqueous dispersion formulation when the average particle diameter is maintained at 0.01 to 5 µm.

In the case of the particle diameter, it can be easily mixed, and even dispersion and dispersion maintenance properties can be exhibited for a considerable time. This can create an advantageous environment for sustaining the cleaning effect and light scattering effect according to the use of the inorganic powder.

3. Mixed Preparation of inorganic powder dispersion

In order to check the synergistic effect of the mixed use of fine powder šœgite, fine powder illite, fine powder zeolite, fine powder elvan stone, fine powder bentonite, fine powder diatomaceous earth, fine powder germanium, fine powder loess, fine powder alum stone and fine powder jade, the following [ Inorganic powder was prepared as shown in Table 4]. On the other hand, the fine powder š-gite, fine powder illite, fine zeolite, fine powder elvan stone, fine powder bentonite, fine powder diatomaceous earth, fine powder germanium, fine powder loess, fine powder alum stone, fine powder jade were to maintain an average particle diameter of 1 to 5 μm. In addition, the inorganic powder was prepared by mixing in a solution containing strontium ions (Sr ^{2 +} ) and calcium ions (Ca ²⁺ ), heat treatment for 1 hour, and then mixing purified water. On the other hand, the inorganic powder is not ion-treated to correspond to T1. By mixing to prepare TX.

T1 T2 T3 T4 T5 T6 T7 T8 T9 Zeolite 100 100 100 100 100 100 100 100 100 šœGuite 100 30 30 30 10 20 40 60 80 Elvan 100 - 30 30 10 20 40 60 80 Bentonite 100 - - 30 10 20 40 60 80 Diatomaceous earth 100 - - - 30 50 75 100 125 germanium 100 - - - 5 10 20 30 40

(Unit: parts by weight)

4. Bacterial inhibitory activity test

In order to confirm the bacterial inhibitory activity for the above preparation example, after preparing a medium to which salt was added, each of T1 to T9 was added, and then Vibrio fluvialis was inoculated and maintained at 23 to 27°C for 24 hours. After culturing, the effect on the growth inhibition of Vibrio fluvialis was measured. The inhibitory effect was expressed in% compared to the control group to which T1 to T9 were not added, and the lower the value, the better the effect on bacterial inhibitory activity.

The results of the cleaning power test and the response test are shown in Table 5 below.

T1 T2 T3 T4 T5 T6 T7 T8 T9 TX Bacterial suppression 89 108 107 91 76 47 52 51 83 142

(Unit: index, %) Referring to [Table 5] above, it can be seen that in the case of T6 to T8, the effect of inhibiting the proliferation of pathogenic bacteria increases due to the synergistic effect by the interaction in the corresponding range. On the other hand, on the other hand, it was confirmed the effect of inhibiting the activity of Vibrio fluvialis in T5 to T9. On the other hand, in the case of TX, it can be seen that the effect of inhibiting bacterial growth is relatively low. Therefore, it can be seen that the inhibitory activity of bacterial growth is increased by the interaction of the inorganic powder and the ion treatment.

Therefore, in the case of the present invention, it can be seen that the survival rate can be increased by enhancing the antibacterial and immunity of the shrimp to be cultured.

5. Inorganic powder dispersion with adsorbed first plant extract

To prepare a plant extract composed of the following [Table 6], The inorganic powder prepared according to Preparation Example T7 was mixed with the plant extract so that the plant extract was adsorbed to the inorganic powder.

On the other hand, the following apricot tree extract, plum tree extract, cotton tree extract, and dandelion extract were prepared by mixing and extracting each apricot tree, plum tree, cotton tree, and dandelion in hot water and filtered.

C1 C2 C3 C4 C5 C6 C7 C8 C9 Apricot tree extract 100 100 100 100 100 100 100 100 100 Plum tree extract - 30 - - 20 30 50 80 100 Cotton tree extract - - 30 - 20 30 50 80 100 Dandelion extract - - - 30 0.5 One 3 5 7

(Unit: parts by weight)

6. Containing the first plant extract Antibacterial activity against inorganic powder Experiment

The plant extracts of C1 to C9 were adsorbed to the T7, and the mixture was injected into each medium by dispersing it in purified water, and the effect of inhibiting the growth of Vibrio fluvialis was confirmed by the same method as the above-described bacterial inhibitory activity test. The results are shown in the following [Table 7].

C1 C2 C3 C4 C5 C6 C7 C8 C9 Bacterial suppression 67 62 52 56 58 41 48 51 66

(Unit: index, %) Referring to [Table 6] above, it can be seen that the activity of inhibiting bacterial growth is increased depending on the impregnated plant extract in the case of C6 to C8. Therefore, it can be seen that when the plant extract is mixed and used, a synergistic effect on the bacterial growth inhibitory activity of the inorganic powder treated in the ionic solution appears.

In particular, it was confirmed that a synergistic effect appears due to the interaction of each plant extract in C6 to C8.

7. Preparation of the second plant extract

After adsorbing the plant extract C7 to the inorganic powder T7, it was dispersed in water, and a second plant extract having a composition as shown in [Table 8] below was mixed. Like the first plant extract, the following dried extract and ganghal extract were prepared by preparing an extract with hot water and filtering.

D1 D2 D3 D4 D5 D6 Dryness extract 100 100 100 100 100 - Kangwha extract - 10 20 60 100 100

(Unit: parts by weight)

8. Containing the second plant extract Inhibition of bacterial growth against inorganic powder Active experiment

The plant extract consisting of D1 to D6 was mixed with C7 adsorbed T7, and the effect of inhibiting the growth of Vibrio fluvialis was confirmed by the same method as the above-described bacterial inhibitory activity test. The results are shown in the following [Table 9].

D1 D2 D3 D4 D5 D6 Antibacterial power 45 39 32 21 38 44

(Unit: index, %) Referring to [Table 9], it can be seen that the activity of inhibiting bacterial growth generally increases when the second plant extract is included. In particular, it can be seen that the inhibitory activity of bacterial proliferation is greatly increased in the case of D3 and D4. It can be seen that in the combination range of D3 and D4, the activity of inhibiting the proliferation of bacteria increases due to a synergistic effect due to the interaction.

Therefore, when the composition containing the inorganic powder is included in the feed and continuously used, the effect of suppressing bacterial growth in the environment in which shrimp are cultured can be achieved.

9. King Jing Spider Shrimp ( Macrobrachium rosenbergii ) Growth promotion effect test

In order to confirm the growth promotion effect of the shrimp according to the present invention, while cultivating the king spider shrimp, the effect on the growth effect was confirmed while using the feed additive for shrimp according to the present invention.

As shown in [Table 10] below, T1, T4, T7, T9, C4, C6, C9, D2, D4, D5 are mixed in 2w% to the total weight of feed while differentiating and cultivating other proteins, carbohydrates and FM1 to FM10 were prepared by mixing in feed for shrimp containing vitamin components. On the other hand, as a control, a feed for shrimp that did not contain the feed additive for shrimp was used.

The size of the giant spider shrimp was 20.48±0.14mm, and individuals of almost the same size were selected, divided into 5 animals, and their growth for 1 month was evaluated as the average mass value of the shrimp. The results are shown in the following [Table 11].

Control FM1 FM2 FM3 FM4 FM5 FM6 FM7 FM8 FM9 FM10 additive - T1 T4 T7 T9 C4 C6 C9 D2 D4 D5

Measurement date Control FM1 FM2 FM3 FM4 FM5 FM6 FM7 FM8 FM9 FM10 2017-5-26 0.19 0.2 0.17 0.18 0.18 0.19 0.21 0.19 0.18 0.17 0.18 2017-6-26 1.21 1.24 1.29 1.41 1.3 1.38 1.52 1.42 1.49 1.63 1.47

(Mass: g) Referring to [Table 11], it was confirmed that there was a slight increase in viability in the case of FM1 and FM2 compared to the control group, but no significant increase in viability was observed.

However, in the case of FM3, it can be seen that the growth rate is greatly increased. It is interpreted that, in the case of the FM3 composition, the growth properties thereof are improved by promoting physiological activity along with the supply of minerals and the increase in the immune area to the king spider shrimp.

Therefore, it can be seen that in the case of the mixing range suggested by the present invention, it is possible to exhibit the effect of increasing the growth properties as well as the antibacterial and immunity enhancing effects against the king spider shrimp.

On the other hand, in the case of FM5, 7, 8, and 10, no significant effect of increasing growth was observed compared to FM3. However, in the case of FM6 and FM9, it can be seen that the effect of promoting the growth of king spider shrimp is remarkably increased. It can be seen that when the first and second plant extracts are mixed and used within a certain range, their growth is promoted by increasing the physiological activity of the giant spider shrimp along with the antibacterial effect.

Therefore, in the case of the present invention, it can be seen that it is possible to increase the yield by increasing the survival rate of the individuals farmed in the step of cultivating shrimp farming, especially the king spider shrimp, and promoting their growth.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements of those skilled in the art using the basic concept of the present invention defined in the following claims are also provided. It belongs to the scope of rights.

Claims (8)

Inorganic powder consisting of a mixture of fine powder š-gite, fine powder zeolite, fine powder elvan stone, fine powder bentonite, fine powder diatomaceous earth and fine powder germanium;
First plant extract; And
It contains a second plant extract,
The first plant extract is a mixture of apricot tree extract, plum tree extract, cotton extract, and dandelion extract,
The second plant extract is a mixture of a dry extract and a ganghal extract
Feed additive for shrimp. According to claim 1,
The inorganic powder has an average particle diameter of 0.005 to 30 μm.
Feed additive for shrimp. delete delete According to claim 1,
The shrimp is a king spider shrimp (Macrobrachium rosenbergii)
Feed additive for shrimp. A dispersion mixing step of preparing a water-dispersible inorganic substance in which an inorganic powder consisting of a mixture of fine powder šœgite, fine powder illite, fine zeolite, fine powder elvan, fine powder bentonite, fine powder diatomaceous earth and fine powder germanium is mixed and dispersed in purified water;
After the dispersion mixing step, comprising a second plant extract mixing step of mixing a second plant extract selected from the group consisting of a dried up extract, a ganghal extract, and a mixture thereof with the water-dispersible inorganic material.
Method for producing feed additives for shrimp. The method of claim 6,
It further comprises a heat treatment step of applying heat treatment to the water-dispersed inorganic material after the dispersion mixing step
Method for producing feed additives for shrimp. delete