KR102625985B1 - Aqua phonics-based feed composition for complex cultivation of agricultural and marine products - Google Patents

Abstract

The present invention relates to an aquaponics-based feed composition for combined cultivation of agricultural and marine products, the feed composition comprising: inorganic powder; protein raw materials; carbohydrate raw materials; vitamin supplements; and a mineral agent, wherein the inorganic powder is a porous particle, into which natural extracts separated from natural products are adsorbed within the internal pores. According to the feed composition of the present invention, the immunity and growth performance of fish can be improved, Afterwards, the growth performance of plants grown in the aquaponics system can also be improved.

Description

Feed composition for aquaponics-based complex cultivation of agricultural and marine products {AQUA PHONICS-BASED FEED COMPOSITION FOR COMPLEX CULTIVATION OF AGRICULTURAL AND MARINE PRODUCTS}

The present invention relates to an aquaponics-based feed composition for combined cultivation of agricultural and marine products. More specifically, the present invention relates to an aquaponics-based feed composition for increasing the immunity and growth of fish by consuming an aquaponics-based feed composition, and to plants that are later grown by the aquaponics system. It relates to a feed composition that can also improve growth performance.

The aquaculture industry's share of the fishing industry is increasing in that it maintains food for the people by providing a stable supply of large quantities of fish and shellfish in response to changes in eating habits while responding to damage to the fishery caused by abnormal weather and changes in fish species in coastal waters.

To meet humanity's increasing consumption of marine products, aquaculture is focusing on mass production through high-density farming. However, due to the artificial high-density aquaculture system, farmed organisms were exposed to various types of stress (high-density farming, physical disturbance, and deterioration of water quality) (Wendelaar Bonga, 1997), and were subsequently infected with various diseases, leading to mass mortality.

As a countermeasure against diseases in farmed fish, antibiotics and various types of chemicals are used after the disease occurs rather than preventing it in advance. The overuse of such drugs not only causes stress to the fish and reduces their immune response, but also causes environmental pollution and increases the resistance of pathogens. It is causing many problems, including the possibility of affecting the human body (Park, 2004).

Meanwhile, aquaponics is a term that combines aquaculture, which is raising fish, and the Greek word ponos, which means growing plants. Aquaponics provides food to fish by supplying nutrients to the tank. This is a method of growing plants using fish excrement and surplus nutrients, allowing you to grow fish and plants at the same time.

In the case of conventional fish feed, it does not provide sufficient phosphorus (P), potassium (K), and iron (Fe) components necessary for plant growth, so adding additional chemicals to help plant growth is recommended. It has been disclosed.

In addition, because terrestrial and aquatic animals cannot efficiently utilize phosphorus (P) in animal and vegetable raw materials, phosphoric acid supplements are added to conventional fish feed to help the growth of fish and plants. Phosphate supplements include, for example, monocalcium phosphate (MCP), dicalcium phosphate (DCP), and tricalcium phosphate (TCP), as well as iron (Chelated iron) and potassium hydroxide ( It may further include chemicals such as KOH), calcium hydroxide (Ca(OH) ₂ ), and dolomite (CaMg(CO ₃ ) ₂ ).

However, if these chemicals are added to supplement the missing trace elements, it may cause problems that worsen the water quality in the tank and cause secondary damage such as environmental pollution, which may limit its practical use as aquaponics feed. there is.

Accordingly, by additionally including natural extracts in the aquaponics-based feed composition for agricultural and marine product complex cultivation of the present invention, the immunity and growth properties of fish farms are improved, while the growth properties of plants produced by excrement discharged from fish farms are also improved. The aim was to provide a feed composition for complex cultivation of agricultural and marine products based on aquaponics.

KR 10-2113974 B1 KR 10-1893030 B1

The object of the present invention is an inorganic powder; protein raw materials; carbohydrate raw materials; vitamin supplements; and a mineral agent, wherein the inorganic powder is a porous particle with natural extracts separated from natural products adsorbed in the internal pores, and improves the immunity and daily growth rate of fish by improving SOD activity, lysozyme activity, weight gain, and daily growth rate. The aim is to provide an aquaponics-based feed composition for combined cultivation of agricultural and marine products that exhibits growth-enhancing activity.

Another object of the present invention is to provide feed for fish farming containing the aquaponics-based feed composition for combined agricultural and marine product cultivation of the present invention.

In order to achieve the above object, an aquaponics-based feed composition for complex cultivation of agricultural and marine products according to an embodiment of the present invention includes inorganic powder; protein raw materials; carbohydrate raw materials; vitamin supplements; and a mineral agent, wherein the inorganic powder is a porous particle, and natural extracts separated from natural products are adsorbed within the internal pores, and the natural products include Hypericum ascyron Linne var. longistylum Max.

The inorganic powder is selected from the group consisting of gaite, illite, zeolite, alumite, bentonite, diatomaceous earth, germanium, red clay, alumite, and mixtures thereof.

The protein raw material is selected from the group consisting of fish meal, blood meal, meat and bone meal, insect meal, soybean meal, cottonseed meal, seed cake, wheat gluten, and mixtures thereof.

The carbohydrate raw material is selected from the group consisting of wheat flour, potato starch, sweet potato starch, corn starch, and mixtures thereof.

The vitamins include vitamin A, vitamin C, vitamin D ₃ , vitamin E _, vitamin B ₁ , vitamin B ₂ , vitamin B 3, vitamin B ₄ , vitamin B ₆ , vitamin B ₉ , vitamin B ₁₂ , and mixtures thereof. is selected from.

The mineral agent is selected from the group consisting of calcium, phosphorus, sodium, chlorine, magnesium, potassium, copper, iodine, iron, manganese, selenium, zinc, and mixtures thereof.

Feed for fish farming according to another embodiment of the present invention is manufactured using the aquaponics-based feed composition for complex cultivation of agricultural and marine products.

Hereinafter, the present invention will be described in more detail.

The term ‘extract’ used in this specification has the meaning commonly used in the art as a crude extract, as described above, but in a broad sense also includes fractions obtained by additional fractionation of the extract. In other words, plant extracts include not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process. For example, fractions obtained by passing the extract through an ultrafiltration membrane with a certain molecular weight cut-off value, separation by various chromatographs (designed for separation according to size, charge, hydrophobicity, or affinity), etc. Fractions obtained through purification methods are also included in the plant extract of the present invention.

The extract used in the present invention can be prepared in powder form by additional processes such as reduced pressure distillation, freeze drying, spray drying, etc.

As used herein, the term ‘active ingredient’ refers to an ingredient that exhibits the desired activity alone or can exhibit activity in combination with a carrier that is not active on its own.

In order to achieve the above object, an aquaponics-based feed composition for complex cultivation of agricultural and marine products according to an embodiment of the present invention includes inorganic powder; protein raw materials; carbohydrate raw materials; vitamin supplements; and a mineral agent, wherein the inorganic powder is a porous particle, and natural extracts separated from natural products are adsorbed within the internal pores, and the natural products include Hypericum ascyron Linne var. longistylum Max.

Aquaponics is a term that combines aquaculture and hydroponics. Aquaponics feeds fish by supplying nutrients to the tank and growing plants through fish excrement and surplus nutrients. As a growing method, it is a sustainable agricultural and fisheries convergence production system that can grow fish and plants at the same time.

More specifically, there is excrement and surplus feed excreted by fish fish in the fish farming tank, and the excrement excreted by the fish fish contains ammonia. The ammonia contains nitrogen necessary for plant growth, but needs to be converted to a nitrate form that plants can absorb. By converting to nitrate, plants can absorb nitrogen, and nitrogen in water is absorbed by plants. It is a circulation system in which water purified by removing ingredients can be returned to the fish farming tank and used as water for fish farming.

Meanwhile, in the aquaponics fish farming tank, general fish feed may be provided for the growth of fish, but in the present invention, aquaponics is used to improve the immunity and growth of fish, as well as to improve the growth of plants to be cultivated later. A nyx-based feed composition for complex cultivation of agricultural and marine products can be provided.

The aquaponics-based feed composition for agricultural and marine product complex cultivation includes inorganic powder; protein raw materials; carbohydrate raw materials; vitamin supplements; and a mineral agent, wherein the inorganic powder is a porous particle, and natural extracts separated from natural products may be adsorbed within the internal pores.

The inorganic powder is selected from the group consisting of gaite, illite, zeolite, alumite, bentonite, diatomaceous earth, germanium, red clay, alumite, and mixtures thereof.

The above-mentioned shungite is a layer (up to 2 m thick) or lens-shaped body in a low-metamorphism shale rich in small graphite microcrystals of the Jatulian period of the Proterozoic Era at Shunga near Suojδrvi, northern Lake Ladoga, Karelia, Russia. do. It is black like pitch, dense, opaque, has a metallic luster, and is soft. It does not melt in the Buddha bowl, but creates graphite acid from concentrated nitric acid.

The illite is a type of clay mineral with a structure similar to mica, and its chemical composition is (K,H ₃ O)Al ₂ (Si,Al) ₄ O ₁₀ (H ₂ O,OH) ₂ . In terms of chemical composition, there is some that is close to muscovite, but it contains relatively more SiO ₂ , MgO, and H ₂ O and less K ₂ O. It is a mineral of the same series as white mica. Hardness is 1 to 2, specific gravity is 2.6 to 2.9. The cleavage is complete as [001], the streak color is white, and there is an earthy gloss. Judging from its chemical composition and crystal structure, there is a view that it is not an independent mineral, but a mixed mineral containing other components. It is produced in aluminum-rich heterogeneous or tuffaceous sedimentary rocks, and as an altered mineral in hydrothermal ore-like host rocks.

The zeolite is also called zeolite. There are many types, but they have commonalities such as high water content, crystal properties, and acid phase. The hardness does not exceed 6, and the specific gravity is about 2.2. It is generally colorless and transparent or white and semi-transparent. It got this name because it boils and expands when heated with a blowpipe. Most types dissolve in hydrochloric acid, often forming a glue-like shape, but a few types do not dissolve in hydrochloric acid. The main types include argillite, fisheye, chabazite, soda arsenite, helandite, stilbite, lomontite, and inesite. It is produced in cavities or rifts of basic igneous rocks such as basalt and pyroxene tuff, and sometimes exists as a secondary mineral in granite and gneiss. In addition, there are cases where it is produced in gold veins and other mineral veins. In terms of the crystal structure, the bonds between each atom are loose, so even if the moisture filling the space is released at high heat, the skeleton remains intact, allowing it to adsorb other fine particles. This property is used as an adsorbent and as a molecular sieve to separate fine particles of different sizes.

The barley stone is known as a term used in Chinese oriental medicine, and geologically classified, it belongs to the granite class. It is similar to quartz porphyry, feldspathic porphyry, and granite porphyry, and its rock name is identical to quartz-monzonite. Quartz and feldspar are closely mixed. The yellowish white, light yellowish brown, light gray, dark green or light green rocks with red or white dots evenly mixed together resemble rice balls made from barley, hence the name Elbanseok. The main ingredients are silicic anhydride and aluminum oxide, and a small amount of ferric oxide is contained. It has 30,000 to 150,000 pores per 1㎤, so it has strong adsorption properties and contains about 25,000 types of inorganic salts. Because it acts to exchange heavy metals and ions, it is also used as a harmful metal remover, and it is known to emit far-infrared rays when heat is applied to this rock.

The bentonite often contains quartz, feldspar, zeolite, etc. The main chemical component is SiO ₂ , and small amounts of Al ₂ O ₃ , MgO, Fe ₂ O ₃ , CaO, and Na ₂ O are contained. Colors include white, gray, light brown, and light green. It is produced as a dense mass with a pearly luster and waxy luster and a fatty feel. It absorbs water and swells, and has clear cation exchange properties, similar to the properties of montmorillonite. Tuff and glassy rhyolite are altered. Its uses are very wide, and it is used as the main ingredient of mud for oil drilling, a binder for castings, an mixing agent for ceramic raw materials, and a base for ointments. The name comes from the strata of Wyoming, USA, where it was discovered.

The diatomite is mainly made of silicic acid (SiO ₂ ) and is white or off-white in color. It is light and soft enough to feel powdery when touched with a finger. The particle size is generally about 10-200㎛, and depending on the particle size, it may be coarse and may have a low density if the pores are large. The general chemical composition of dried diatomaceous earth is about 80-90% silica, about 2-4% alumina, and about 0.5-2% iron oxide, and alumina is mainly influenced by clay minerals. Because it is finely porous, it has strong absorption properties and is a poor conductor of heat.

The germanium has the element symbol Ge, atomic number 32, and atomic weight 72.59±3. Its existence was predicted by D. I. Mendeleev in 1871 and it was called ekasilicon, but in 1886, it was discovered in silver germanium stone (AgGeS) by German chemist C. A. Winkler and was named after the Latin name for Germany, “Germania.” Because it is similar to silicon, it exists widely in the earth's crust in place of silicon in silicates. It is also contained in sulfide minerals and coal containing copper and zinc, but there are very few minerals that mainly contain germanium.

The loess is a light yellow sediment composed mainly of silt-sized particles, loosely cemented together by calcium carbonate. Red clay is usually homogeneous, has no developed stratification, and has large porosity. Additionally, vertical fractures have developed that split the sedimentary layer vertically. The particle size corresponding to the size of red clay is 0.02 to 0.05 mm and includes coarse-grained and medium-grained dust. According to particle size analysis using various methods, this size ratio is about 50% by weight. Particles of clay size (less than 0.005 mm) make up 5 to 10%. The mineral composition of red clay is as follows. It contains 60-70% quartz, and its content varies from a minimum of 40% to a maximum of 80%. Feldspar and mica make up 10 to 20%, and carbonate minerals make up 5 to 35%. Approximately 2-5% of silt is composed of heavy minerals such as amphibole, apatite, biotite, chlorite, disten (kyanite), chlorite, garnet, glauconite, pyroxene, rutile, sillimanite, achyranite, tourmaline, and zircon, and the particles are Typically slightly weathered. In the fine-grained particle size (less than 0.002 mm), clay minerals such as montmorillonite, illite, kaolinite, etc. are superior to clastic (fragmentary) minerals. Clay minerals are created through various colloidal or physicochemical processes during or after red clay is accumulated. The mineral composition of red clay is very homogeneous, but varies slightly due to differences in particle size and origin.

The alunite is a basic sulfate mineral of aluminum and potassium, and was named as a shortened version of the previous name given to this material, aluminilite. Mineral minerals are produced and altered through the action of albino petrification, that is, astragalus, and are widely produced. In addition, there are examples of semi-earthy rocks being turned into albino by the action of sulfuric acid produced from the oxidation of pyrite. This action is often performed in parallel with kaolinization and silicification actions. The chemical composition is KAl ₃ (SO ₄ ) ₂ (OH) ₆ , and the physical properties are good for splitting and poor for splitting. The cross section is shell-shaped and soft, the hardness is 3.5 to 4, the diameter is 2.75 to 2.82, and the colors are white, gray, yellow, red, and reddish brown, and have a glassy luster. Additionally, it exhibits strong pyroelectricity.

The inorganic powder described above is included to improve the immunity and growth of fish, and the type is not limited, but may include gate and illite, and more specifically, gate and illite. It may be included at 2:1, but is not limited to that weight ratio.

The inorganic powder may be one from which impurities in the internal pores have been removed, and the inorganic powder from which the impurities in the internal pores have been removed may contain an extract of Hypericum ascyron Linne var. longistylum Max. in the internal pores.

The Hypericum ascyron Linne var. longistylum Max. is a perennial herb of the dicotyledonous plant family Asteraceae and grows in sunny places in mountains and fields. It is about 1m tall, and the stem is strong and straight like a tree and has four ridges. The leaves are opposite, lancet-shaped, overall larger than those of a water plant, have sharp tips, the lower part surrounds the stem, and there are transparent spots on the edges. The flowers bloom in yellow from June to July, and grow one at a time at the end of each branch, and are larger than those of the water plant. The calyx is divided into 5 pieces and is egg-shaped with many veins, and the petals are curved egg-shaped. There are five styles, about 1cm long, longer than the stamens, and the ends are split by about one-third. The fruit is a capsule that is egg-shaped and ripens in November. The young leaves are eaten as a vegetable and the fully grown leaves are used medicinally in the private sector for swelling, wounds, etc. Distributed in Korea, Japan, China, Ussuri River, Heilong River, and Siberia.

The aquaponics-based feed composition for complex cultivation of agricultural and marine products can exhibit immunity and growth-enhancing activities in fish farming by including an extract of the aquaponics plant in the inorganic powder, as well as the growth potential of plants later cultivated in the aquaponics system. It can also be improved.

Preferably, the natural extract that can be included in the inorganic powder may further include Gabari ginseng extract and Jindeukchal extract in addition to the above-mentioned Euphorbiaceae extract.

The Lapsana apogonoides is a biennial herb of the dicotyledonous plant Campanula Asteraceae and is also called soybean paste ttukgal or barley pepper. It grows near rice fields and is 4 to 20 cm tall. There is a lot of hair all over, but it gradually disappears, and the soft stems grow into clumps and branch out. The leaves on the roots are bunched together and spread out in all directions, and the leaves on the stems are alternate, and the leaves are long oval shaped like dandelion leaves. One to three leaves on the stem fall off from each other, and the lower leaves are similar to the leaves on the roots. The leaves are 4 to 10 cm long and 1 to 2 cm wide, and gradually become smaller toward the top. Yellow flowers bloom from March to June. At first, they appear as a loose corymb, but as the branches grow, they droop down, and the flower stalk is 1.5 to 5 cm long. The involucre is cone-shaped, the outer segments are short and hairy, the inner segments are five, and the edges are membranous (translucent like thin paper). There are 6 to 9 small flowers and the corolla is 5 to 6 mm long. The fruit is an achene, 3 to 4.5 mm long, long oval, and brown. Distributed in Korea (Jeju, Jeonnam, Jeonbuk), Japan, and central China.

The Siegesbeckia glabrescns Makino. is an annual herbaceous plant belonging to the Asteraceae family, commonly growing in fields or near fields, and growing about 1m in height. The leaves are ovate triangular, 5 to 13 cm long, 3.5 to 11 cm wide, and have irregular sawtooth edges. The leaves become smaller as they go up, becoming long oval or linear. The flowers are yellow and bloom in August and September, and the fruit is an achene and has an obovate shape. The above Jindeukchal is also called Heeryeomcho, Hwaryeom, Jeogomae, Hwaheomcho, etc. In Oriental medicine, the whole plant is used as medicine. The medicinal effect is to lower blood pressure due to its vasodilating effect, and is effective for quadriplegia, musculoskeletal pain, weakness in the waist and knees, and acute hepatitis. Patients with high blood pressure can also take it as tea.

Preferably, the feed composition further includes inorganic powder, in addition to the Euphorbia chinensis extract, an extract of Gabaria chinensis and an extract of Jindeukchal, or when it contains only the Euphorbia chinensis extract due to the synergistic effect of the mixed use of the natural extracts. Compared to this, the immune and growth enhancing activity of fish can be improved, and the growth of plants grown in the aquaponics system can also be improved.

More preferably, the feed composition may include 10 to 30 parts by weight of Euphorbia chinensis extract, 10 to 30 parts by weight of Gabaria ginseng extract, and 5 to 15 parts by weight of Jindeukchal extract, based on 100 parts by weight of the inorganic powder.

When included within the above weight part range, a synergistic effect of critical significance is expressed due to the synergistic effect due to the interaction of each component, and when outside the above range, the synergistic effect is sharply reduced or almost non-existent.

The extract is extracted with a solvent selected from the group consisting of water, C ₁ to C ₆ alcohol, and mixtures thereof.

The method of preparing the extract may be a conventional extraction method in the art, such as ultrasonic extraction, leaching, and reflux extraction. Specifically, it may be an extract obtained by extracting a natural product from which foreign substances have been removed by washing and drying with water, alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, and may be an extract obtained by sequentially applying the solvents to the sample.

The ultrasonic extraction method proceeds at 30 to 50°C for 0.5 to 2.5 hours, and the extraction solvent is water or 50 to 100% alcohol with 1 to 6 carbon atoms. Specifically, extraction is performed at 40 to 50°C for 1 to 2.5 hours, and the extraction solvent is water or 70 to 80% alcohol with 1 to 6 carbon atoms.

The leaching method is carried out at 15 to 30°C for 24 to 72 hours, and water or 50 to 100% alcohol with 1 to 6 carbon atoms is used as the extraction solvent. More specifically, the extraction is performed at 20 to 25°C for 30 to 54 hours, and the extraction solvent is water or 70 to 80% alcohol with 1 to 6 carbon atoms.

The reflux extraction method is based on 100 mL of water and alcohol with 1 to 6 carbon atoms, 10 to 30 g of pulverized natural product, reflux time of 1 to 3 hours, and 50 to 100% of alcohol or water with 1 to 6 carbon atoms. More specifically, based on 100 mL of alcohol with 1 to 6 carbon atoms or 100 mL of water, 10 to 20 g of pulverized natural product, 1 to 2 hours of reflux time, and 70 to 90% of alcohol or water with 1 to 4 carbon atoms.

The extraction solvent can be used in an amount of 2 to 50 times, preferably 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left in the extraction solvent for 1 to 48 hours, specifically 1 to 24 hours.

After extraction, the extract can be fractionated by sequentially applying a new fractionation solvent. The fractionating solvent used during fractionation is at least one selected from the group consisting of water, hexane, butanol, ethyl acetic acid, ethyl acetate, methylene chloride, and mixtures thereof, and is preferably ethyl acetate or methylene chloride.

After obtaining the extract or fraction, additional methods such as concentration or freeze-drying can be used.

The protein raw material is selected from the group consisting of fish meal, blood meal, meat and bone meal, insect meal, soybean meal, cottonseed meal, seed cake, wheat gluten, and mixtures thereof.

The feed composition of the present invention may contain all of the above protein raw materials, but is not limited thereto, and preferably includes insect powder, fish meal, and blood meal in a ratio of 2:2:1.

The insect powder is made by drying and pulverizing insects. Insects have a high protein content, grow quickly, and have a high reproduction rate, enabling high-density intensive production, so they are preferably used as protein raw materials for fish farming. There are no restrictions on the types of insects that can be used, and any type that can supplement protein in fish farming can be used.

The fish meal is made by steaming and pressing the whole or part of the fish, removing the juice, drying and pulverizing the solid portion. Brown meal is made from red-fleshed fish, and white meal is made from white-fleshed fish. It is called white meal. It is mainly used as a raw material for compound feed given to livestock or farmed fish.

The blood meal, also called dried blood, is made by heating, coagulating, and compressing blood from a slaughterhouse, dehydrating it, drying it, and pulverizing it. It is dark brown in color and contains about 12% of nitrogen in the form of proteins such as fibrin and albumin. It decomposes relatively quickly and has a high fertilizer effect.

Accordingly, the aquaponics-based feed composition for agricultural and marine product complex cultivation of the present invention can supply protein ingredients necessary for aquaculture fish by containing the insect powder, fish meal, and blood meal in a ratio of 2:2:1.

The carbohydrate raw material is selected from the group consisting of wheat flour, potato starch, sweet potato starch, corn starch, and mixtures thereof.

Fish's main food consists of animal matter such as plankton and crustaceans, so through the process of evolution, their digestive system has evolved to use protein and fat as their main energy source, and fish recovers the increased blood sugar level due to carbohydrate intake to a normal level. It takes a considerable amount of time to do this.

Accordingly, carbohydrates do not supply essential nutrients to fish, and excessive carbohydrates may cause digestive or metabolic disorders. Therefore, carbohydrate raw materials may be included primarily to serve as a binder to mold the feed source.

The aquaponics-based feed composition for combined cultivation of agricultural and marine products of the present invention preferably contains potato starch and wheat flour in a ratio of 1:1, but is not limited thereto.

The vitamins include vitamin A, vitamin C, vitamin D ₃ , vitamin E _, vitamin B ₁ , vitamin B ₂ , vitamin B 3, vitamin B ₄ , vitamin B ₆ , vitamin B ₉ , vitamin B ₁₂ , and mixtures thereof. is selected from.

Vitamin supplements are essential components to improve the growth of fish and resistance to disease. Fish cannot synthesize vitamins in their bodies, so they require vitamin intake through feed. Additionally, supplying feed lacking vitamins may cause changes in body constitution and death.

Accordingly, the present invention may include a vitamin agent selected from the group consisting of a mixture of the above-described vitamin agents, but preferably vitamin A, vitamin C, vitamin D ₃ , vitamin E, vitamin B ₁ , vitamin B ₂ , and vitamin B. ₆ and vitamin B ₁₂ , but is not limited thereto, and the necessary vitamin ingredients can be added or subtracted at the level of a person skilled in the art.

The mineral agent is selected from the group consisting of calcium, phosphorus, sodium, chlorine, magnesium, potassium, copper, iodine, iron, manganese, selenium, zinc, and mixtures thereof.

In the case of natural fish, mineral supplementation is not essential as they receive essential minerals from the sea, their habitat, but in the case of farmed fish, mineral supplementation is essential.

Accordingly, in the present invention, it may be selected from the group consisting of a mixture of the above-described mineral agents, but preferably may include calcium, sodium, potassium, manganese, and zinc, but is not limited thereto, and the necessary mineral agents may be added or subtracted. can do.

Additionally, although not described above, the feed composition for aquaponics-based agricultural and marine product complex cultivation of the present invention may further include a lipid source.

Fish require specific fatty acids to maintain cell function in the body, but these fatty acids cannot be synthesized by the fish's body itself, so they must be supplied through feed.

The lipid source may include fish oil containing polyunsaturated fatty acids EPA and DHA, and the fish oil is divided into fish body oil obtained from gizzard shad and liver oil collected from soy sauce.

Preferably, the present invention may include liver oil, especially squid liver oil. The squid liver oil is made from soy sauce and other intestines that are produced as by-products when processing dried squid and other squid, and can be collected by self-digestion using enzymes contained in the intestines.

In addition, known feed additives, corn gluten, fish meal adsorbed feed, etc. can be further included by appropriately selecting an effective or desirable amount to enhance functionality, such as improving the growth environment, survival rate, and growth efficiency of fish.

Preferably, the aquaponics-based feed composition for growing agricultural and marine products of the present invention contains 40 parts by weight of inorganic powder, 20 parts by weight of carbohydrate raw material, 20 parts by weight of lipid source, 10 parts by weight of vitamin agent, and 10 parts by weight of mineral agent, based on 100 parts by weight of protein raw material. It may include weight parts.

Feed for fish farming according to another embodiment of the present invention is manufactured using the aquaponics-based feed composition for complex cultivation of agricultural and marine products.

The feed can be broadly divided into three methods based on moisture content, production method, and sinking or floating feed.

First, depending on moisture content, it can be divided into dry feed and wet feed. The dry feed (Extruded pellet, EP) is produced using a machine called an extruder and has a low moisture content, so it is less likely to spoil and can be stored for a long period of time. The wet feed (Moist pellet, MP) has a higher moisture content than the dry feed.

Second, depending on the production method, it can be divided into raw feed and compounded feed. The raw feed is feed made by grinding fish to create a certain shape, but it has the disadvantage of being easily dissolved in water. Meanwhile, the formula feed is a feed manufactured containing essential and trace nutrients, protein sources, etc. required for fish. It does not dissolve easily in water compared to raw feed, so it can reduce water pollution, and it can reduce water pollution when producing feed. The nutrient content required can be adjusted to help fish grow, and it is easy to store and manage.

Thirdly, it can be divided into floating pellets and sinking pellets. Depending on their characteristics, there are species of fish that eat floating or sinking feed. Therefore, appropriate feed must be provided according to the intake characteristics of fish.

The feed for fish farming provided by the present invention can provide feed that matches the intake characteristics of fish, but is not limited to the types of feed described above.

In addition, as shown in Figure 4 below, the aquaponics system of the present invention largely includes a fish farming unit (100); Bio-processing unit (200); and a plant cultivation unit 300.

The fish farming unit 100 is a structure capable of cultivating fish for fish farming, and has a shape where the upper side is open so that fish can be cultured inside. Its size can be changed depending on the number of fish farms, and the number is also It may consist of one or more.

It is preferable that the fish cultured in the fish farming unit 100 are fish that are not sensitive to ammonia and dissolved oxygen, but are not limited thereto.

The bio-processing unit 200 is configured to convert fish excrement contained in the aquaculture water transferred from the fish farming unit so that plants can absorb it and supply it to the plant cultivation unit. Inside the bio-processing unit 200, the fish excrement is contained. It may include a filter adsorbed by microorganisms that can be converted into components that plants can absorb.

The plant cultivation unit 300 is configured to grow plants using aquaculture water supplied from the bio processing unit 200. The upper side of the plant cultivation unit 300 can cultivate plants, and the lower side is capable of cultivating plants. Aquaculture water supplied from the processing unit 200 may flow.

The plant cultivation unit 300 may be formed with a plant cultivation plate 310 on which plants can be grown and a plant cultivation area 311 disposed at regular intervals on the plant cultivation plate 310, The lower part of the unit 300 may be configured with a plant cultivation water storage unit 320 through which water for plant cultivation flows.

In addition, the aquaponics-based agricultural and marine product complex cultivation system 10 of the present invention may further include an oxygen supply unit 700.

The oxygen supply unit 700 supplies oxygen in the air, and supplies oxygen to the fish farming unit 100 and the bio-processing unit 200, thereby supplying oxygen to fish and nitrifying bacteria to breathe and grow by oxygen. It is a configuration that allows you to do so.

The bio processing unit includes a bio filter therein.

The bio-processing unit 200 includes a bio-filter 210 attached with microorganisms that convert fish waste in the aquaculture water transferred from the fish farming unit 100 so that plants can absorb it. The biofilter 210 may have a plurality of filters installed depending on the fish farming unit 100 and the number of fish accommodated.

The biofilter is characterized by converting ammonia in fish excrement contained in aquaculture water transported from the fish farming department into nitrate.

Specifically, there is excrement and surplus feed discharged from fish fish inside the fish farming unit 100, and in particular, the excrement discharged from the fish fish contains ammonia. The ammonia contains nitrogen necessary for plant growth, but when ammonia itself is introduced to plants, it is toxic and can be fatal to plant growth. Therefore, the ammonia in the excrement discharged from fish farming needs to be converted into a nitrate form that can be absorbed by plants.

The nitrate (NO ₃ ^- ) is a substance produced during the nitrification process of ammonia (NH _3, NH ₄ ^- ), and is carried out sequentially by two types of nitrifying bacteria. First, ammonia (NH _3, NH ₄ ^- ) is converted into nitrite (NO ₂ ^- ) by ammonia-oxidizing bacteria, and then nitrate (NO ₃ ^- ) is produced through nitrite-oxidizing bacteria. Through the nitrification process, toxic ammonia (NH ₃ ) is converted into less toxic nitrate (NO ₃ ^- ), which can be used as a nitrogen source for plants.

The ammonia-oxidizing bacteria (AOB) include Nitrosomonas, Nitrosospira (β-Proteobacteria), and Nitrosococcus (γ-Proteobacteria), and the nitrite-oxidizing bacteria (NOB) include Nitrospira (Nitrospirae), It is divided into four groups: Nitrobacter (α-Proteobacteria), Nitrococcus (γ-Proteobacteria), and Nitrospina (δ-Proteobacteria).

In the present invention, Nitrosomonas is included as ammonia-oxidizing bacteria, and Nitrobacter (α-Proteobacteria) is included as nitrite-oxidizing bacteria, but it is not limited thereto and can be changed as needed at the level of a person skilled in the art. .

Accordingly, the bio filter 210 installed inside the bio processing unit 200 of the present invention can attach nitrifying bacteria to convert ammonia discharged from fish into nitrate form, which is not toxic to plants, and provide it as a nutrient source.

In addition, the aquaponics-based agricultural and marine product complex cultivation system of the present invention may further include a culture water storage unit 400.

The aquaculture water storage unit 400 is composed of a aquaculture water storage unit A (410) and aquaculture water storage unit B (420), and the aquaculture water storage unit A (410) is comprised of the bio-processing unit 200 and the plant cultivation unit. (300), and the aquaculture water storage unit B (420) may be configured between the plant cultivation unit (300) and the fish farming unit (100).

The aquaculture water storage unit A (410) is a place where aquaculture water is temporarily stored before the aquaculture water treated from the bio-processing unit 200 is provided to the plant cultivation unit 300. Ammonia (NH ₃ , NH ₄ ^- ) By measuring the concentration of ammonia (NH ₃ , NH ₄ ^- ), if the concentration is high, the aquaculture water is provided back to the bio-processing unit 200 to lower the ammonia (NH ₃ , NH ₄ ^- ) concentration in the aquaculture water. It is configured to do this.

In addition, the aquaculture water storage unit B (420) is a place where the aquaculture water treated from the plant cultivation unit 300 is temporarily stored before it is provided back to the fish aquaculture unit 100, and nitrate (NO ₃ ^- ) By measuring the concentration of nitrate (NO 3 ^- ), if the concentration of nitrate (NO ₃ - ) is high, the aquaculture water is provided back to the plant cultivation unit 300 to lower the nitrate (NO ₃ ^- ) concentration in the aquaculture water. .

A concentration measuring unit 600 capable of measuring the concentration of ammonia (NH ₃ , NH ₄ ^- ) or nitrate (NO ₃ ^- ) may be installed inside the aquaculture water storage unit 400.

The concentration measuring unit 600 consists of a concentration measuring unit A (610) and a concentration measuring unit B (620), and the aquaculture water storage unit A (410) and the aquaculture water storage unit B (420) each contain ammonia (NH It may include a concentration measuring unit A (610) capable of measuring ₃ , NH ₄ ^- ) concentration and a concentration measuring unit B (620) capable of measuring nitrate (NO ₃ ^- ) concentration.

The aquaponics-based agricultural and marine product complex cultivation system further includes a feed supply unit, wherein the feed supply unit includes a feed storage tank; and a feed supply pipe; wherein the feed supply pipe transfers feed from the feed storage tank and provides feed to the fish farming unit.

The aquaponics-based agricultural and marine product complex cultivation system of the present invention can automatically supply feed to fish, thereby reducing the labor required to administer feed.

The feed supply unit 500 includes a feed storage tank 510 and a feed supply pipe 520, and the feed stored in the feed storage tank 510 is supplied to the fish farming unit 100 through the feed supply pipe 520. It can be supplied by, and the feed supply pipe is controlled through the control unit 800.

The feed supply unit 500 may further include a power unit 530 and a feed supply setting unit 540, and the power unit 530 and the feed supply setting unit 540 may be controlled through the control unit 800. there is.

The power unit 530 can set the power of the feed supply unit to ON/OFF through an ON/OFF switch (not shown). Through this, the user can adjust the settings depending on whether automatic feeding of feed is needed. In addition, the feed supply setting unit 540 can set and adjust the time, frequency, and amount of feed supplied from the feed supply unit 500 to the fish farming unit 100.

Meanwhile, the feed storage tank contains the aquaponics-based feed composition for complex cultivation of agricultural and marine products of the present invention.

When cultivating fish and plants by adding the aquaponics-based feed composition for agricultural and marine products complex cultivation, the growth and immunity of fish cultivated with the aquaponics-based agricultural and marine products complex cultivation system of the present invention are improved, and the growth properties of plants are improved. Additionally, improved agricultural and marine products can be grown.

The aquaponics-based feed composition for agricultural and marine product complex cultivation of the present invention can increase the immunity and growth of fish, while also improving the growth of plants grown thereafter.

The present invention can provide a feed containing the aquaponics-based feed composition for combined cultivation of agricultural and marine products.

Figure 1 shows the results of an experiment for evaluating the intracellular toxicity of an extract of Euphorbia chinensis according to an embodiment of the present invention.
Figure 2 shows the results of an experiment for evaluating the intracellular toxicity of the Gabori Pyeonggi extract according to an embodiment of the present invention.
Figure 3 shows the results of an experiment on the evaluation of intracellular toxicity of the extract of Jindeukchal extract according to an embodiment of the present invention.
Figure 4 is a block diagram of an aquaponics-based agricultural and marine product complex cultivation system according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement it. However, the present invention may be implemented in many different forms and is not limited to the embodiments described herein.

[Manufacture Example 1: Preparation of aquaponics-based feed composition for combined cultivation of agricultural and marine products]

1. Production of inorganic powder (CL)

Gite and illite are mixed at a weight ratio of 2:1, ground to an average diameter of 3 to 6㎛, and a standard deviation of the average diameter of 1 to 2㎛, and then mixed with purified water to dissolve in the internal pores. After removing impurities, it was dried and made into inorganic powder (CL).

2. Production of natural extracts

2-1. Preparation of Euphorbiaceae extract (HT)

The snails were washed cleanly, dried, and then pulverized using a grinder. Distilled water, an extraction solvent, was added to the sample at a ratio of 1:10 (w:v), then completely immersed, and extraction was repeated 3 times for 3 hours while refluxing at 80°C. The extract was Whatman No. 2 Filtered with filter paper. The filtrate was concentrated under reduced pressure at 60°C to prepare an extract (HT).

2-2. Preparation of other extracts

Gabori ginseng extract (LT) and Jindeukchal extract (ST) were prepared using the same method as the preparation method for the above-mentioned T. chinensis extract (HT).

3. Manufacturing of inorganic powder with natural extracts adsorbed

The prepared inorganic powder (CL) and natural extracts (HT, LT, ST) were mixed as shown in Table 1 below to prepare inorganic powders (C1 to C11) mixed with natural extracts.

C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11 CL 100 100 100 100 100 100 100 100 100 100 100 HT - One 10 20 30 40 20 20 20 20 20 L.T. - - - - - - One 10 20 30 40 S.T. - - - - - - One 5 10 15 20

(Unit: parts by weight)

4. Preparation of feed composition

An aquaponics-based feed composition for complex cultivation of agricultural and marine products as shown in Table 2 below, including protein raw materials, carbohydrate raw materials, vitamins, minerals and lipid sources in the inorganic powder (C1 to C11) mixed with natural extracts prepared in Table 1 above ( S1 to S11) were prepared.

S1 S2 S3 S4 S5 S6 S7 S8 S9 S10 S11 inorganic powder C1 C2 C3 C4 C5 C6 C7 C8 C9 C10 C11

(Protein ingredients are insect powder, fish meal and blood meal in a 2:2:1 ratio, carbohydrate ingredients are potato starch and wheat flour in a 1:1 ratio, lipid sources are squid liver oil, vitamins are vitamin A, vitamin C, vitamin _D3 , vitamin E, Vitamin B ₁ , Vitamin B ₂ , Vitamin B ₆ and Vitamin B ₁₂ , minerals include calcium, sodium, potassium, manganese and zinc, and for 100 parts by weight of protein raw materials, 40 parts by weight of inorganic powder, 20 parts by weight of carbohydrates. , Includes 20 parts by weight of lipid source, 10 parts by weight of vitamins, and 10 parts by weight of minerals.)

[Experimental Example 1: Evaluation of cytotoxicity of natural extracts in inorganic powder]

1. Cell culture

RAW264.7 cells used DMEM medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, and experiments were conducted by subculturing every 2-3 days.

2. Measurement of cell survival rate by treatment with Euphorbiaceae extract (HT)

A cytotoxicity measurement test was conducted to evaluate the safety of the HT extract (HT) included in the aquaponics-based feed composition for agricultural and marine product complex cultivation. RAW264.7 cells were distributed at 1.5Х10 ⁵ cells/well in a 96 well plate and cultured for 24 hours. Before the experiment, the culture medium was replaced with a new one, and the extracts of Euphorbiaceae were treated with concentrations of 1, 10, and 100 ㎍/㎖, respectively, and cultured again for 24 hours. After incubation, 10 ㎕ of EZ-Cytox solution was added and reacted in a cell incubator for 30 minutes. After reaction, the change in absorbance was measured at 450 nm, and the cell viability relative to the control group was expressed as a percentage, and the results are shown in Figure 1 below.

Referring to Figure 1 below, it was confirmed that the extract (HT) contained in the aquaponics-based feed composition for combined agricultural and marine product cultivation of the present invention does not exhibit toxicity to cells.

3. Measurement of cell viability by other extract treatments

In the same manner as the cell viability evaluation of the above-described cellulose extract (HT), a cytotoxicity evaluation experiment was conducted on the Gabaria ginseng extract (LT) and the root extract (ST), and the results are shown in Figures 2 and 3 below. .

Referring to Figures 2 and 3 below, it was confirmed that both the Gabori Pyeonggi extract (LT) and the Jindeukchal extract (ST) were not toxic to cells.

[Experimental Example 2: Evaluation of trout immunity and growth of feed compositions containing H. trout extract (HT)]

1. Breeding of trout

Trout were preliminarily reared in a 200L tank by providing basic feed for 10 days.

Afterwards, 15 trout with an average fish weight of 6.13 ± 0.13 g (mean ± SD) were housed in a 60 L square tank and randomly placed, and the general fish farming feed and the feed composition of the present invention were mixed in a ratio of 3:7 and provided as feed. The average water temperature was maintained at 15±0.5℃, and an air stone was installed to ensure sufficient oxygen supply. The daily feed amount was 3 to 3.3% of the fish's body weight, three times a day during the entire experiment period, and the breeding experiment was conducted for a total of 6 weeks. In addition, for comparison of the experimental results, Comparative Example 1 was prepared by providing only general fish farming feed without including the feed composition of the present invention.

2. Biometrics

In all experiments, the population and body weight were measured every two weeks to measure weight gain, feed efficiency, daily growth rate, protein conversion efficiency, and survival rate.

- Weight gain rate (WG, %) = (final weight - initial weight) × 100% / (initial weight)

- Feed efficiency (FE, %) = (wet weight gain / dry feed intake) × 100%

- Daily growth rate (SGR, %) = (log _e final weight - log _e initial weight) × 100% / (experiment performance period)

- Protein conversion efficiency (PER) = (wet weight gain / protein intake)

- Survival rate (%) = (Total number of fish - Number of dead fish) × 100% / (Total number of fish)

After rearing for the final 6 weeks, the results of measuring weight gain, feed efficiency, daily growth rate, protein conversion efficiency, and survival rate are shown in Table 3 below.

division Comparative Example 1 S1 S2 S3 S4 S5 S6 Increase rate (%) 209.18 214.84 220.66 236.19 240.11 242.54 222.44 Feed efficiency (%) 94.13 94.64 96.13 98.51 99.08 99.12 96.47 Daily growth rate (%) 2.49 2.53 2.61 2.78 2.81 2.83 2.64 Protein conversion efficiency 0.42 0.43 0.43 0.45 0.46 0.46 0.43 Survival rate (%) 90.1 91.3 93.5 96.4 96.7 96.8 93.7

According to Table 3, it can be seen that the weight gain rate, feed efficiency, daily growth rate, protein conversion efficiency, and survival rate of S1 to S6 are all improved compared to Comparative Example 1, and in particular, it contains a preferable weight portion of the L. chinensis extract. In the case of S3 to S5, it can be seen that more improved result values are shown.

3. Analysis of general components of whole fish

Using randomly extracted and ground gizzard gizzard shad from each tank, according to the AOAC (Association of Official Analytical Chemists, 2000) method, moisture was determined by normal pressure heat drying (135°C, 2 hours) and crude protein was determined by Kjeldahl nitrogen determination method (N 6.25), the views were analyzed using the direct conversation method. Crude fat was analyzed by Soxhlet extraction using a soxtec system 1046 (Tacator AB, Sweden) after freeze-drying the sample for 12 hours, and the results are shown in Table 4 below.

division Comparative Example 1 S1 S2 S3 S4 S5 S6 Moisture (% by weight) 73.11 73.61 72.84 72.05 72.16 73.01 72.84 Protein (% by weight) 18.24 18.28 18.59 18.83 18.9 18.85 18.64 Lipid (% by weight) 7.76 7.72 7.68 7.53 7.42 7.51 7.69 Ash content (% by weight) 1.74 1.76 1.72 1.73 1.75 1.77 1.73

Referring to Table 4, it can be seen that the nutrients are almost similar as a result of component analysis, and in particular, the protein content of S1 to S6 that consumed the feed composition of the present invention showed a higher protein content compared to Comparative Example 1, In particular, it was confirmed that S3 to S5 were superior.

4. Blood measurement experiment

The blood components of fish can be used to interpret nutrition, health status, and stress. They also change depending on the lack of essential nutrients in the feed or the habitat environment and growth in which the fish species is located. Inorganic powders or natural extracts in the feed Blood components were examined to evaluate the effect of the addition of the included inorganic powder on the physiological state of the experimental fish.

After completing the 6-week breeding experiment, the experimental fish were fasted for about 24 hours before collecting blood for blood composition analysis. Then, 3 fish from each tank were randomly selected and blood was injected from the caudal vessel within 30 seconds using a blood syringe (1 mL). was collected. A portion of the collected blood was centrifuged (3,000 RPM for 10 minutes) to separate serum, and glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), glucose, and total cholesterol were analyzed using a commercial kit. ) was used to analyze with DRI-CHEM 4000i- Fuji Dri-Chem Slide- 3150 (Minato-ku, Tokyo, Japan), and the results are shown in Table 5 below.

division Comparative Example 1 S1 S2 S3 S4 S5 S6 GOT(U/L) 21.6 21.5 21.3 20.1 19.5 19.4 21.2 GPT(U/L) 22.1 21.8 21.6 21.3 20.1 20.9 21.6 Glucose (mg/dL) 38.4 35.9 33.2 31.5 31.1 31.9 33.1 T-Cholesterol (mg/dL) 122.8 121.1 120.8 117.6 120.4 121.7 122.9

- GOT: Glutamic oxaloacetic transaminase. One unit is defined as the amount of enzyme causing the transamination of 1.0 μmol of L-aspartate per minute at 25℃ and pH 7.4

- GPT: Glutamic pyruvic transaminase. One unit is defined as the amount of enzyme causing the transamination of 1.0 μmol of L-alanine per minute at 25℃ and pH 7.4

Referring to Table 5 above, there was no significant difference in GOT, GPT, and T-Cholesterol in plasma, but in the case of glucose, compared to Comparative Example 1, the feed composition containing H. sp. extract (HT) and inorganic powder was significantly higher. It was confirmed that the glucose content was lower than that of S1 to S6, and in particular, the glucose content of S3 to S5 was lower than that of S3 to S5. In most animals, including fish, the glucose content increases when exposed to an external stress environment, and through the above experiment, it can be confirmed that the degree of stress in fish farming caused by the feed composition of the present invention is not high.

5. Non-specific immune response experiment

5-1. SOD activity measurement experiment

With serum isolated from fish in each experimental group, the enzyme inhibition rate was calculated as a percentile using WST-1 (Water Soluble Tetrazolium dye) and xanthine oxidase using the SOD Assay Kit (Sigma-Aldrich, 191600) following the manufacturer's instructions.

Then, when each sample was reacted in an incubator at 37°C for 20 minutes and reached parallel time, the absorbance was measured at 450 nm wavelength (absorbance of the wavelength to measure the color produced by the reaction of WST-1 and active oxygen). Measure. The inhibition rate was expressed in SOD activity units per mg protein, and the results are shown in Table 6 below.

5-2. Lysozyme activity measurement experiment

0.1 ml of serum isolated from fish in each experimental group was mixed with 2 ml of suspension in which Micrococcus lysodeikticus (0.2 mg/ml) was suspended in 0.05M sodium phosphate buffer (pH 6.2). And, the reaction was measured at 0.5 and 4.5 minutes at 530 nm in a spectrophotometer at 20°C. The activity unit of lysozyme was defined as the amount of enzyme that resulted in a decrease in absorbance of 0.001 per minute. The measurement results are shown in Table 6 below.

division Comparative Example 1 S1 S2 S3 S4 S5 S6 SOD (% inhibition) 36.88 40.84 43.06 50.99 51.01 50.84 43.11 Lysozyme (U/ml) 0.81 0.85 0.91 1.16 1.18 1.16 0.94

Macrophages increase reactive oxygen species (ROS) by hydrolyzing bacteria and viruses that invade the body. SOD activity is a representative antioxidant enzyme that catalyzes the reaction that changes peroxide ions into oxygen and hydrogen peroxide. It plays a role in removing pathogens that invade the body (Fattman et al., 2003), and lysozyme is a lymphocyte-derived mucolytic enzyme that exhibits antibiotic-like properties and becomes part of the endogenous defense mechanism (Ingram, 1980).

Referring to Table 6, in the SOD activity experiment, S3 to S5 were significantly higher than Comparative Example 1, which was the control. Looking at the lysozyme activity results, S3 to S5 were significantly higher than Comparative Example 1, which was the control. appear.

Through this, it was confirmed that the feed composition of the present invention improves SOD activity and Lysozyme activity, thereby enhancing the immunity of fish.

[Experimental Example 3: Evaluation of trout immunity and growth improvement of feed composition containing complex extract]

In order to evaluate the immunity and growth improvement activity of feed compositions S7 to S11 containing an inorganic powder further containing the extract of Gabaricum ginseng and the extract of Jindeukchal extract of the present invention, the same test method as in Experimental Example 2 was carried out. In order to compare the experimental results, the experimental value of S4 conducted in Experimental Example 2 was fixed at an exponent of 5 and evaluated within the range of exponents 1 to 10.

The biometric measurement results for S7 to S11 are comprehensively shown in Table 7 below, and the blood measurement test and non-specific immune response test results are comprehensively shown in Table 8 below.

In addition, the closer the result of SOD and Lysozyme in Tables 7 and 8 below to an index of 10, the better the growth potential and non-specific immune response. The result values of GOT, GPT, Glucose and T-Cholesterol in Table 8 below indicate The closer the index is to 1, the better the physiological condition of the fish.

division S4 S7 S8 S9 S10 S11 increase rate 5 7 9 9 10 7 feed efficiency 5 6 9 10 10 7 Daily growth rate 5 6 9 10 9 6 Protein conversion efficiency 5 5 8 9 9 6 survival rate 5 6 8 9 8 7

(Unit: index)

division S4 S7 S8 S9 S10 S11 GOT(U/L) 5 4 2 One One 2 GPT(U/L) 5 4 2 One 2 2 Glucose (mg/dL) 5 4 3 2 2 3 T-Cholesterol (mg/dL) 5 3 One One 2 2 SOD (% inhibition) 5 6 8 9 9 5 Lysozyme (U/ml) 5 6 8 9 8 6

(Unit: index)

Referring to Table 7 and Table 8 above, in the case of the aquaponics-based feed compositions S7 to S11 for combined cultivation of agricultural and marine products of the present invention, the weight gain rate of fish and feed compared to S4 containing only Leopardium chinensis extract (HT) in inorganic powder It was confirmed that efficiency, growth rate, protein conversion efficiency, and survival rate all showed improved index values.

It was confirmed that better results were shown in the physiological state and non-specific immune response of fish.

In particular, in the case of S8 to S10 containing a preferred weight portion of the above-mentioned Achyranthes extract (HT), Aerobium ginseng extract (LT), and a ginseng extract (ST), the activity is significantly improved compared to S4, S7, and S11. It was confirmed that this was the case.

As a result, this means that the aquaponics-based feed composition for agricultural and marine product complex cultivation of the present invention can enhance the growth and immunity of farmed fish.

[Experimental Example 4: Evaluation of plant growth improvement in aquaponics system by administration of feed composition of the present invention]

Prepare the feed compositions S4, S8 to S10 of the present invention and general fish feed (Comparative Example 1), respectively, and select 200 to 240 g of trout as the fish species and 2 types of kale and romaine (30 plants per type) as the plants to increase the plant cultivation area. Plant growth through a 4 m ² aquaponics system was analyzed for 6 weeks, and the results are shown in Table 9 below.

division Comparative Example 1 S4 S8 S9 S10 plant species Kale Average (g) 30.8 34.8 37.6 40.2 38.7 Total (g) 924 1044 1128 1206 1161 romaine Average (g) 25.3 28.4 31.8 35.4 32.4 Total (g) 759 852 954 1062 972

(Unit: g)

Referring to Table 9, it can be seen that when the feed compositions S4, S8 to S10, which are the feed compositions of the present invention, are used compared to Comparative Example 1, better plant growth is observed, and the kale and romaine of Comparative Example 1 The total total was 924g and 759g, respectively, confirming that it showed a greater plant growth effect than in the case of the present invention.

Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the following claims are also possible. falls within the scope of rights.

10: Aquaponics-based agricultural and marine product complex cultivation system
100: Fish farming department
200: Bioprocessing department
210: biofilter
300: Plant cultivation department
310: Plant cultivation plate
311: Plant cultivation area
320: Plant cultivation water storage unit
400: Aquaculture water storage unit
410: Aquaculture water storage unit A
420: Aquaculture water storage unit B
500: Feed supply department
510: Feed storage tank
520: Feed supply pipe
530: power unit
540: Feed supply setting unit
600: Concentration measurement unit
610: Concentration measuring unit A
620: Concentration measuring unit B
700: Oxygen supply unit
800: Control unit

Claims (7)

inorganic powder; protein raw materials; carbohydrate raw materials; vitamin supplements; and mineral agents,
The inorganic powder is a porous particle, and natural extracts separated from natural products are adsorbed within the internal pores,
The natural products include Hypericum ascyron Linne var. longistylum Max.
Aquaponics-based feed composition for combined cultivation of agricultural and marine products. According to paragraph 1,
The inorganic powder is selected from the group consisting of gite, illite, zeolite, alumite, bentonite, diatomaceous earth, germanium, red clay, alumite, and mixtures thereof.
Aquaponics-based feed composition for combined cultivation of agricultural and marine products. According to paragraph 1,
The protein raw material is selected from the group consisting of fish meal, blood meal, meat and bone meal, insect meal, soybean meal, cottonseed meal, seed cake, wheat gluten, and mixtures thereof.
Aquaponics-based feed composition for combined cultivation of agricultural and marine products. According to paragraph 1,
The carbohydrate raw material is selected from the group consisting of wheat flour, potato starch, sweet potato starch, corn starch, and mixtures thereof.
Aquaponics-based feed composition for combined cultivation of agricultural and marine products. According to paragraph 1,
The vitamins include vitamin A, vitamin C, vitamin D ₃ , vitamin E _, vitamin B ₁ , vitamin B ₂ , vitamin B 3, vitamin B ₄ , vitamin B ₆ , vitamin B ₉ , vitamin B ₁₂ , and mixtures thereof. selected from
Aquaponics-based feed composition for combined cultivation of agricultural and marine products. According to paragraph 1,
The mineral agent is selected from the group consisting of calcium, phosphorus, sodium, chlorine, magnesium, potassium, copper, iodine, iron, manganese, selenium, zinc, and mixtures thereof.
Aquaponics-based feed composition for combined cultivation of agricultural and marine products. Containing the aquaponics-based feed composition for complex cultivation of agricultural and marine products according to any one of claims 1 to 6.
Feed for fish farming.