CN103431197B - Method for preparing solid feed additive by transforming cordyceps sinensis fermented residue by using EM (Effective Microorganisms) flora - Google Patents
Method for preparing solid feed additive by transforming cordyceps sinensis fermented residue by using EM (Effective Microorganisms) flora Download PDFInfo
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- CN103431197B CN103431197B CN201310396164.6A CN201310396164A CN103431197B CN 103431197 B CN103431197 B CN 103431197B CN 201310396164 A CN201310396164 A CN 201310396164A CN 103431197 B CN103431197 B CN 103431197B
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Abstract
The invention relates to a method for preparing a solid feed additive by transforming cordyceps sinensis fermented residue by using EM (Effective Microorganisms) flora. The method comprises the following steps of treating a fermented residue, separating an EM strain, preparing an EM activated solution and propagating the EM flora. The prepared solid feed additive can be applied to the cultivation of various animals. The cordyceps sinensis fermented residue is transformed by using the EM flora to obtain the solid feed additive rich in EM probiotics, the problem of recycling the cordyceps sinensis fermented residue is solved, and better environmental protection benefit and economic benefit are obtained.
Description
Technical field
The present invention relates to the recycling field of Cordyceps sinensis fermentation residue, is that a kind of EM of utilization flora transforms the method that Cordyceps sinensis fermentation residue is prepared solid feed additive specifically.
Background technology
Cordyceps sinensis claims again Chinese caterpillar fungus, is China tradition rare Chinese medicine, has cough-relieving, reduces phlegm, reduces serum cholesterol, adjusting and enhancing immunity, the multiple pharmacological effect such as antitumor.In recent years, both at home and abroad Cordyceps sinensis demand is increased severely.But because Cordyceps sinensis has its strict parasitics and special growth geographical environment, output is quite limited, and medicine source is more in short supply.
For alleviating the imbalance between supply and demand in market, artificial fermentation's product of Cordyceps sinensis is more and more important.Deep Fermentation of Cordyceps sinensis Sacc production substitute, both can effectively protect these precious resources of Cordyceps sinensis, and the not strict restriction of climate, geographical environment and parasitic conditions be again suitable for large-scale industrialization and produce, thereby development in recent years is very rapid.But the utilization of this substitute mainly concentrates on ferment gained mycelium or fermenation raw liquid at present, for the recycling of the bulk fermentation residue producing in cultivating, rarely has report.Conventionally aborning, the fermentation raffinate that accounts for the large portion of tunning can be used as general waste liquid and enter environment, has caused the waste of worm grass resources.And fermentation raffinate is rich in several amino acids, active nitrogen, carbohydrate, be the hotbed of various mushroom breedings, at natural purification process, will inevitably grow a large amount of bacterium and smelly, environment is caused to very big pollution.Take in a large number corn flour and the silkworm chrysalis that in sweat, produce are that main residue also abandons with waste formation.For this reason, carry out the demand of fermentation residue conversion or recycling extremely urgent.
Wang Hui medium (" constituent analysis of caterpillar fungus cephalosporin fermented waste fluid and recycling thereof ", < < China Environmental Science > >) caterpillar fungus cephalosporin zymotic fluid composition is measured and analyzed, discovery includes multiple nutritional components, completely likely again utilizes; And study this zymotic fluid and cultivate after treatment Trichoderma harzianum, have advantages of that sporulation quantity is high, it is low to cultivate cost.
Guo Xuhui etc. (" comprehensive utilization of artificial aweto zymotic fluid and prospect ", < < Guizhou Agricultural Sciences > >) propose, utilize Chinese caterpillar fungus fermentation waste water to carry out mycelial production, both can alleviate environmental pollution, can utilize again that the fermentation of utility in fermented waste fluid makes new advances fodder yeast---single cell protein is as animal feed.Propose simultaneously, utilize Chinese caterpillar fungus fermentation waste liquid to carry out secondary fermentation, cultivate the fungi strain specifically with insecticidal action, as paecilomyces fumosoroseus, Paecilomyces cicadae etc. is applied to warmhouse booth.
Liu Tianmei (" cultivation of Hirsutella sinensis and effective constituent determination thereof and waste liquid comprehensive utilizating research ' '; Sichuan Agricultural University's master thesis) with the Hirsutella sinensis fermented waste fluid spice yellow meal worm of feeding; research shows still to contain and can, for the active ingredient of utilizing, have certain nutritional utilization and be worth in Hirsutella sinensis fermented waste fluid.
But in prior art, do not utilize EM flora to transform the report of Cordyceps sinensis fermentation residue.
EM is the abbreviation language of English (Effective Microorganisms), and Chinese is translated into " CEM ".It belongs to more than 80 by 5 sections 10 such as photosynthetic bacteria group, lactobacillus, yeast flora, actinomycete group, thread flora and plants the composition of the microorganism.Each quasi-microorganism utilizes the material of other microorganisms, forms a kind of coexistence and co-prosperity system, thereby guarantees that EM probio multi-effective active liquid status is stable, multiple functional.So this complex micro organism fungicide can be brought into play several functions at aspects such as plantation, cultivation, environmental protection, promote growth and development of plants and animals, suppress disease and occur.
Due to EM flora propagation, need to add the basic ingredients such as carbohydrate, cellulose, mineral matter and trace element, and the main component of the fermentation raffinate of Cordyceps sinensis is carbohydrate, protide, amino acids, trace element etc., the propagation hotbed of EM flora just, and fermentation residue be take corn flour and silkworm chrysalis as main, so the fermentation residue of Cordyceps sinensis is the ideal culture medium matter of EM flora propagation, thereby also makes Cordyceps sinensis fermentation residue obtain trans-utilization.
Summary of the invention
The object of the present invention is to provide a kind of EM of utilization flora to transform the method that Cordyceps sinensis fermentation residue is prepared solid feed additive.The method is utilized Cordyceps sinensis fermentation residue, realized the propagation of EM flora, both can alleviate environmental pollution, the solid feed additive that can utilize again the utility fermentation in fermentation residue to make new advances, thereby the problem that has solved the recycling of Cordyceps sinensis fermentation residue, has obtained good environmental benefit and economic benefit.
The invention provides a kind of EM of utilization flora and transform the method that Cordyceps sinensis fermentation residue is prepared solid feed additive, it is characterized in that, comprise the following steps:
(1) processing of fermentation residue: get the fermentation raffinate after Deep Fermentation of Cordyceps sinensis Sacc, measure water content after adding fermentation residue, obtain propagation matrix, standby;
(2) separation of EM bacterial classification: separated photosynthetic bacteria group, lactobacillus, yeast flora, actinomycete group and thread flora from the EM bacterium liquid of buying;
(3) preparation of EM active liquid: the photosynthetic bacteria group of described separation, lactobacillus, yeast flora, actinomycete group and thread flora are mixed by weight ratio, make EM active liquid;
(4) propagation of EM flora: described propagation matrix is placed in to fermentation tank, adds described EM active liquid and stir and evenly mix, sealing propagation, obtains solid feed additive.
Preferably, described in step (1), water content is 3-10%; More preferably 5-8%, is further preferably 6%.
Preferably, described in step (3), weight portion proportioning is photosynthetic bacteria group: lactobacillus: yeast flora: actinomycete group: thread flora=0.2-1.2: 1.5-2.5: 6-10: 0.5-1.3: 2-4; Photosynthetic bacteria group more preferably: lactobacillus: yeast flora: actinomycete group: thread flora=0.6: 2: 8: 0.8: 3.
Preferably, described in step (4), breeding temperature is 20-40 ℃; 25-35 ℃ more preferably; Further be preferably 28 ℃.
Preferably, described in step (4), generation time is 7-28 days; More preferably at 20-28 ℃ of temperature, breed 16-28 days, at 28-40 ℃ of temperature, breed 7-16 days.
Preferably, the composition of fermentation raffinate comprises, in dry product, amino acid whose percentage by weight is 28.97 ± 2.10%, polysaccharide is 6.63 ± 1.40%, sweet mellow wine is 0.32 ± 0.20%, the residual total reducing sugar that ferments is 0.58 ± 0.30%, remaining ammonia nitrogen is 18 ± 2.50%; Further preferably, amino acid whose percentage by weight is 28.97%, polysaccharide is 6.63%, sweet mellow wine is 0.32%, the residual total reducing sugar that ferments is 0.58%, remaining ammonia nitrogen is 18%.
Described solid feed additive can be applicable to, in the cultivation of various animals, particularly be applied to domestic animal, poultry, to improve average daily gain, improves animal resistance, and environment is played to catharsis.
Described photosynthetic bacteria group energy is utilized the solar thermal energy of soil acceptance or be take ultraviolet ray as the energy, hydrogen in hydrogen sulfide in soil and hydrocarbon is separated, and to take the secretion, pernicious gas etc. of plant root be matrix, synthetic carbohydrate, amino acid, vitamins, nitrogen compound and physiological activator etc.; Described lactic acid bacteria group energy produces lactic acid, thereby effectively suppresses harmful microbe activity, and promotes organic sharply corrupt decomposition; Described yeast flora is produced and is promoted fissional activate material by fermentation energy, for other effective microbe propagation provides important nutrition guarantee, and energy manufacture order cell protein; Described actinomyces group energy produces various antibiotics, vitamin and enzyme, can directly suppress pathogen, and the material that difficulty is decomposed has degradation, strengthens animals and plants to the resistance of various diseases and immunity, also can promote nitrogen-fixing bacteria and VA VA Mycorrhizal Fungi propagation; Described der Pilz group energy prevents the generation of maggot and other insects, and can eliminate stench.
The invention has the beneficial effects as follows and utilize EM flora to transform Cordyceps sinensis fermentation residue, acquisition contains the solid feed additive that enriches EM probio, both can alleviate environmental pollution, fermentation makes new solid feed additive again, thereby the problem that has solved the recycling of Cordyceps sinensis fermentation residue, has obtained good environmental benefit and economic benefit.
The specific embodiment
Embodiment 1
Utilize EM flora to transform the method that Cordyceps sinensis fermentation residue is prepared solid feed additive, it is characterized in that, comprise the following steps:
(1) processing of fermentation residue: get the fermentation raffinate after Deep Fermentation of Cordyceps sinensis Sacc, add that to measure water content after fermentation residue be 3%, obtain propagation matrix, standby;
(2) separation of EM bacterial classification: separated photosynthetic bacteria group, lactobacillus, yeast flora, actinomycete group and thread flora from the EM bacterium liquid of buying;
(3) preparation of EM active liquid: the photosynthetic bacteria group of described separation, lactobacillus, yeast flora, actinomycete group and thread flora are pressed 0.2: 1.5: 10: weight portion proportioning was mixed in 0.5: 2, made EM active liquid;
(4) propagation of EM flora: described propagation matrix is placed in to fermentation tank, adds described EM active liquid and stir and evenly mix, sealing propagation, propagation temperature is 20 ℃, generation time is 28 days, obtains solid feed additive.
Embodiment 2
Utilize EM flora to transform the method that Cordyceps sinensis fermentation residue is prepared solid feed additive, it is characterized in that, comprise the following steps:
(1) processing of fermentation residue: get the fermentation raffinate after Deep Fermentation of Cordyceps sinensis Sacc, add that to measure water content after fermentation residue be 10%, obtain propagation matrix, standby;
(2) separation of EM bacterial classification: separated photosynthetic bacteria group, lactobacillus, yeast flora, actinomycete group and thread flora from the EM bacterium liquid of buying;
(3) preparation of EM active liquid: the photosynthetic bacteria group of described separation, lactobacillus, yeast flora, actinomycete group and thread flora are pressed 1.2: 2.5: 6: weight portion proportioning was mixed in 1.3: 4, made EM active liquid;
(4) propagation of EM flora: described propagation matrix is placed in to fermentation tank, adds described EM active liquid and stir and evenly mix, sealing propagation, propagation temperature is 40 ℃, generation time is 7 days, obtains solid feed additive.
Embodiment 3
Utilize EM flora to transform the method that Cordyceps sinensis fermentation residue is prepared solid feed additive, it is characterized in that, comprise the following steps:
(1) processing of fermentation residue: get the fermentation raffinate after Deep Fermentation of Cordyceps sinensis Sacc, add that to measure water content after fermentation residue be 6%, obtain propagation matrix, standby;
(2) separation of EM bacterial classification: separated photosynthetic bacteria group, lactobacillus, yeast flora, actinomycete group and thread flora from the EM bacterium liquid of buying;
(3) preparation of EM active liquid: the photosynthetic bacteria group of described separation, lactobacillus, yeast flora, actinomycete group and thread flora are pressed 0.6: 2: 8: weight portion proportioning was mixed in 0.8: 3, made EM active liquid;
(4) propagation of EM flora: described propagation matrix is placed in to fermentation tank, adds described EM active liquid and stir and evenly mix, sealing propagation, propagation temperature is 28 ℃, generation time is 16 days, obtains solid feed additive.
The constituent analysis of test example 1 fermentation raffinate
Respectively get the fermentation raffinate after Deep Fermentation of Cordyceps sinensis Sacc in 1L embodiment 1-3, carry out concentrated spray dry, the gross weight of gained dry product is 5.69 ± 2.50g, utilize high performance liquid chromatography, Autoanalysis of free aminoacids and spectrophotometric spectra method that gained dry product is measured and analyzed, main component and percentage by weight are wherein as follows:
| Main component | Percentage by weight is (in dry product, %) |
| Total amino acid content | 28.97±2.10 |
| Polysaccharide | 6.63±1.40 |
| Sweet mellow wine | 0.32±0.20 |
| Residual total reducing sugar ferments | 0.58±0.30 |
| Remaining ammonia nitrogen | 18±2.50 |
Test example 2 impacts of solid feed additive on poultry average daily gain
The solid feed additive that embodiment 1-3 is made adds in normal diet, and is applied to, in the cultivation of chicken (meat chick), duck (young sheldrake) and goose (young goose), 100 every group, average daily gain respectively compare with control group.Wherein, the chicken of control group, duck, goose are only fed the normal diet that does not add solid feed additive, and other cultivating condition of test group and control group is identical, and be 15 days experimental period.The results are shown in following table:
As seen from the above table, the solid feed additive application that embodiment 1-3 is made in the cultivation of chicken, duck, goose after, the average daily gain of three kinds of poultry is significantly improved.
Test example 3 impacts of solid feed additive on domestic animal average daily gain
The solid feed additive that embodiment 1-3 is made adds in normal diet, and is applied to, in the cultivation of pig (family pig), ox (beef cattle) and sheep (little tail tremble with fear sheep), average daily gain respectively compare with control group.Wherein, the pig of control group, ox, sheep are only fed the normal diet that does not add solid feed additive, and other cultivating condition of test group and control group is identical, and be 30 days experimental period.The results are shown in following table:
As seen from the above table, the solid feed additive application that embodiment 1-3 is made in the cultivation of pig, ox, sheep after, the average daily gain of three kinds of domestic animals is significantly improved.
Test example 4 impacts of solid feed additive on domestic animal premunition
In the livestock culturing process of test example 3, pig (family pig), ox (beef cattle) and sheep (little tail tremble with fear sheep) are carried out to resistance against diseases observation, result shows, after feeding and support with the feed that contains the solid feed additive that embodiment 1-3 makes, none is ill or dead for domestic animal, also without thin and weak phenomenon; And in control group, have respectively 21%, 15% and 28% pig, ox, sheep that dysentery, the stomach slow or disease such as have loose bowels of speeding occurred, even there is 3% sheep because resistance against diseases is compared with weak and dead.
The catharsis of test example 5 solid feed additives to environment
In the poultry of test example 2 and 3, livestock culturing process, test also finds, all feed the poultry of feed that contains solid feed additive, in the colony house of domestic animal stink lighter, fly is also less.This is because EM contains the beneficial microbes such as photosynthetic bacteria, there is stronger nitrogen fixing capacity, enter after enteron aisle, can competitive exclusion or suppress the corrupt flora in former enteron aisle, stop the intrusion of external harmful bacteria, reduce enteron aisle internal protein to the conversion of ammonia or amine, add that beneficial microbe makes ight soil and blood ammonia mass fraction decline effect etc. to reducing animal waste stink in enteron aisle, environment purification has good effect.
Finally it should be noted that, the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (15)
1. utilize EM flora to transform the method that Cordyceps sinensis fermentation residue is prepared solid feed additive, it is characterized in that, comprise the following steps:
(1) processing of fermentation residue: get the fermentation raffinate after Deep Fermentation of Cordyceps sinensis Sacc, measure water content after adding fermentation residue, obtain propagation matrix, standby;
(2) separation of EM bacterial classification: separated photosynthetic bacteria group, lactobacillus, yeast flora, actinomycete group and thread flora from the EM bacterium liquid of buying;
(3) preparation of EM active liquid: the photosynthetic bacteria group of described separation, lactobacillus, yeast flora, actinomycete group and thread flora are mixed by weight ratio, make EM active liquid;
(4) propagation of EM flora: described propagation matrix is placed in to fermentation tank, adds described EM active liquid and stir and evenly mix, sealing propagation, obtains solid feed additive;
Wherein, described in step (3), weight portion proportioning is photosynthetic bacteria group: lactobacillus: yeast flora: actinomycete group: thread flora=0.2-1.2: 1.5-2.5: 6-1O: 0.5-1.3: 2-4.
2. method according to claim 1, is characterized in that, described in step (1), water content is 3-10%.
3. method according to claim 2, is characterized in that, described in step (1), water content is 5-8%.
4. method according to claim 2, is characterized in that, described in step (1), water content is 6%.
5. method according to claim 1, is characterized in that, described in step (3), weight portion proportioning is photosynthetic bacteria group: lactobacillus: yeast flora: actinomycete group: thread flora=0.6: 2: 8: 0.8: 3.
6. method according to claim 1, is characterized in that, described in step (4), breeding temperature is 20-40 ℃.
7. method according to claim 6, is characterized in that, described in step (4), breeding temperature is 25-35 ℃.
8. method according to claim 6, is characterized in that, described in step (4), breeding temperature is 28 ℃.
9. method according to claim 1, is characterized in that, described in step (4), generation time is 7-28 days.
10. method according to claim 9, is characterized in that, generation time described in step (4) is to breed 16-28 days at 20-28 ℃ of temperature.
11. methods according to claim 9, is characterized in that, generation time described in step (4) is to breed 7-16 days at 28-40 ℃ of temperature.
12. according to the method described in claim 1-11 any one, it is characterized in that, the composition of fermentation raffinate comprises, in dry product, amino acid whose percentage by weight is 28.97 ± 2.10%, polysaccharide is 6.63 ± 1.40%, sweet mellow wine is 0.32 ± 0.20%, the residual total reducing sugar that ferments is 0.58 ± 0.30%, remaining ammonia nitrogen is 18 ± 2.50%.
13. methods according to claim 12, is characterized in that, amino acid whose percentage by weight is 28.97%, polysaccharide is 6.63%, sweet mellow wine is 0.32%, the residual total reducing sugar that ferments is 0.58%, remaining ammonia nitrogen is 18%.
The application of the solid feed additive that described in 14. claim 1-13 any one, method makes in the cultivation of various animals.
15. application according to claim 14, is characterized in that, described animal is livestock or poultry.
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| CN106173208A (en) * | 2016-07-20 | 2016-12-07 | 太仓海斯生态农业专业合作社 | A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the method for antibiotic in feedstuff |
| CN108142359B (en) * | 2017-12-29 | 2021-06-04 | 合肥迈可罗生物工程有限公司 | Method for improving reproductive performance of sows |
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| KR100350093B1 (en) * | 2002-03-29 | 2002-08-24 | Jewoo Corp | Feed additive for increase of the physiological activity of bred fish and promotion of the growth thereof using effective microorganisms and medicinal herbs, and manufacturing method thereof |
| KR101168658B1 (en) * | 2010-05-14 | 2012-07-25 | 경북대학교 산학협력단 | Probiotics and fermenting livestock feed with probiotics and by-product of agricultual product |
| CN101999515A (en) * | 2010-11-19 | 2011-04-06 | 江苏东宝粮油集团有限公司 | Process for producing basal feed for replacing grain crops by using active bacteria preparation zymophyte bran |
| CN103125737A (en) * | 2011-11-29 | 2013-06-05 | 张立民 | Method for feed made of edible fungus culture medium waste material |
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Non-Patent Citations (2)
| Title |
|---|
| 王慧中，等.虫草头孢菌发酵废液成分分析及其再利用.《中国环境科学》.2000,第20卷(第2期),第180-183页. * |
| 郭旭辉，等.人工虫草发酵液的综合利用与展望.《贵州农业科学》.2002,第30卷(第5期),第58-61页. * |
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Address after: 810001 Xining biological science and Technology Industrial Park, No. two, No. 16, Qinghai Patentee after: Wang Hui Address before: 810001 No. 19, Dongxin Road, Xining economic and Technological Development Zone, Qinghai, China Patentee before: Wang Hui |
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Effective date of registration: 20170724 Address after: 810001 Xining biological science and Technology Industrial Park, No. two, No. 16, Qinghai Patentee after: Qinghai Mount Everest Cordyceps Engineering Technology Co., Ltd. Address before: 810001 Xining biological science and Technology Industrial Park, No. two, No. 16, Qinghai Patentee before: Wang Hui |
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