CN106173208A - A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the method for antibiotic in feedstuff - Google Patents

A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the method for antibiotic in feedstuff Download PDF

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Publication number
CN106173208A
CN106173208A CN201610570254.6A CN201610570254A CN106173208A CN 106173208 A CN106173208 A CN 106173208A CN 201610570254 A CN201610570254 A CN 201610570254A CN 106173208 A CN106173208 A CN 106173208A
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lactic acid
acid bacteria
substitute
vegetalitas
bacterium
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张扬
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Taicang Hayes Eco Agriculture Cooperatives
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Taicang Hayes Eco Agriculture Cooperatives
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Priority to CN201610570254.6A priority Critical patent/CN106173208A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/125Casei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/179Sakei

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

The invention provides and a kind of utilize vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute the method for antibiotic in feedstuff, belong to the production method of a kind of microorganism formulation, described method is, use the bean cake pulverized that vegetalitas lactic acid bacteria is spread cultivation, then according to the ratio of 1:10 mixes with the EM bacterium using clear bran to spread cultivation, keep humidity 60% ~ 70% to complete additive and prepare.Compared with the simple additive using EM bacterium, vegetalitas lactic acid bacteria is acidproof and bile tolerance ability is higher, and can survive under the adverse circumstances that nutrition is uneven, enters intestinal survival rate higher, and colonization ability is higher.It is remarkably improved beneficial microbe retaining portion in poultry intestinal tract, thus in terms of improving intestinal environment, raising immunity advantageously.

Description

A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute antibiotic in feedstuff Method
Technical field
The invention belongs to animal feed additive field, a kind of substitute antibiotics strengthens the feedstuff of immunity of poultry Addictive preparation method.
Background technology
Along with the progress of society with to healthy attention, green cultivation product is increasingly favored by consumers in general, But traditional intensive culture easily causes the most popular of poultry disease, therefore typically all uses interpolation antibiosis in feedstuff The mode of element prevents disease.The antibiotic content thus causing Fowl meat exceeds standard, and people knows from experience after eating the certain negative effect of generation. In order to avoid the negative effect of antibiotic, the feed formula of domestic more existing producer research antibiotic-free.
Such as patent 201310167917 .6, a kind of Chinese medicine mixing chicken feed and preparation method thereof, proposition Chinese medicine with raise The way of material mixed fermentation;Patent 201510077055.7, a kind of antibiotic-free chicken feed addictive and preparation method thereof, propose Use Chinese herbal medicine is as additive, and gives the formula of Chinese herbal medicine;And for example patent 201610062450.2, a kind of antibiotic-free The preparation method of type of fattening chicken feed propose use EM bacterium fermented feed, and add Mulberry bar, Fructus Momordicae, hundred Herba Pelargonii Graveolentiss fruits etc. The method of Chinese herbal medicine substitute antibiotics.
Although more than invention can obtain positive effect, but is limited to source and the processing mode of herbal raw material, opposing Easily there is fluctuation by a relatively large margin in the effect of disease, and processing process complex too increases cost, limiting it should With universal.
EM bacterium is the various bacteria combination with good function researched and developed by Japanese Scientists.But micro-life of composition EM bacterium Thing mostly is aerobic or facultative kind, therefore uses EM bacterium limited as the additive Use Limitation fruit improving immunity when simple.
Vegetalitas lactic acid bacteria is prevalent in the class in traditional vegetalitas fermented foods such as Pickles, sauerkraut, broad bean paste Lactic acid bacteria, including Lactobacillus plantarum, lactobacillus casei, Lactobacillus brevis etc..Compared with animality lactic acid bacteria, vegetalitas lactic acid bacteria is resistance to Acid and bile tolerance ability are higher, and can survive under the adverse circumstances that nutrition is uneven, enter intestinal survival rate higher, colonization ability Higher.Therefore it is improving intestinal environment, improve in terms of immunity advantageously.Therefore the product having commercialization is used for improving people Body intestinal health and raising immunity.
Experiment shows, vegetalitas lactic acid bacteria can form good symbiosis with the multiple beneficial microorganism in EM bacterium, Strengthen and feed with resident in poultry intestinal tract of EM bacterium, and then strengthening poultry intestinal tract microorganism species is for the opposing of poor environment Ability.Therefore, by correct combination, it is provided that the preparation side of a kind of simple and easy to do antibiotic-free livestock fodder additives Method.
Summary of the invention
It is an object of the invention to provide one utilizes vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute antibiotic in feedstuff Method.
The present invention is achieved through the following technical solutions:
A kind of utilizing vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute the method for antibiotic in feedstuff, described method is to use bean Spread cultivation vegetalitas lactic acid bacteria being added on of the dregs of rice uses in the EM bacterium that spreads cultivation of clear bran, and both ratios are 1:10, control humidity 60% ~ 70%, airtight save backup.
Preferably, described vegetalitas lactic acid bacteria culturers is lactobacillus germ, picogram lactobacillus probiotic bacteria-65, cheese Lactobacillus one or more mixture therein.
Preferably, after vegetalitas lactobacillus inoculation and bean cake powder are pressed the weight ratio mixing and stirring of 1:50, add water tune Joint humidity is to 60% ~ 70%, and then under conditions of fully sealing, normal temperature fermentation completes the expansion of plant lactobacillus thalline for more than 15 days Training.
Preferably, by EM strain after brown sugar water activates 2 ~ 3 hours, admix dry smart bran according to weight ratio 1:100 and mix After closing uniformly, the regulation mixture humidity that adds water is to 60 ~ 70%, and room temperature lower seal completes spreading cultivation of EM thalline after 72 ~ 80 hours.
Preferably, described plant lactobacillus strain can use the Semen Sojae Preparatum abrasive material completely that ferments to substitute.
The invention has the beneficial effects as follows: experiment shows, feed house using EM bacterium+vegetalitas mixture of lactic acid bacteria to add Fowl, can make the survival rate raising poultry scattered be promoted to more than 82% from 70%, and effect is clearly.Fully demonstrating this mixture can To obtain good enhancing immunity of poultry, the effect of defending and fighting against diseases.
1, compared to brown sugar activation or cultivate, the clear bran of humidity 60% ~ 70% do not change EM bacterium composition in the case of, Significantly delay decaying of EM bacterium;
2, add vegetalitas lactic acid bacteria at traditional E M strain to strengthen, improve beneficial microbe retention in poultry intestinal tract Ratio, thus improves feedstuff absorbance and the resistance against diseases of poultry;
3, described operational approach is easy, with low cost.Raw materials used easily obtain in rural area.Can commonly use.
Detailed description of the invention
A kind of utilize vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute the method for antibiotic in feedstuff, be by following step Rapid realization:
A: tame the vegetalitas lactic acid bacteria that spreads cultivation.Bean cake powder is broken to powder, then accesses vegetalitas lactic acid bacteria culturers, by strain With bean cake powder according to the weight ratio mixing and stirring of 1:50 after, add water regulation humidity to 60% ~ 70%, then fully sealing Under conditions of normal temperature fermentation within 15 days, complete to spread cultivation, standby.
B: spread cultivation EM bacterium, after being activated 2 hours with brown sugar water by EM bacterium, admixes dry clear bran according to weight ratio 1:100 (clear bran is second time grinding product after Oryza sativa L. is shelled, and mainly contains substantial amounts of Oryza sativa L. plumule, seed coat and the mixing of rice flour Thing) in, the regulation mixture humidity that adds water subsequently is to 60% ~ 70%, and room temperature lower seal completes to spread cultivation for 72 hours, standby.
C: take vegetalitas mixture of lactic acid bacteria 1 part prepared by step A and add EM bacterium prepared by step B and spread cultivation product 9 parts mixing, Mix homogeneously, and regulate the humidity of mixture to 60% ~ 70%, prepared by the additive i.e. completing substitute antibiotics.This additive can Guarantee the quality 3 months under normal temperature condition under air-proof condition.
In the present embodiment, thing lactic acid bacteria culturers can use the Semen Sojae Preparatum abrasive material completely that ferments to substitute.
During use, take additive prepared by preceding method according to the ratio of 2% and add in feedstuff, or 1% ratio by spice Add in the drinking water of poultry, or both use simultaneously.
Experiment shows, is using EM bacterium+vegetalitas mixture of lactic acid bacteria to add feed for poultry, can make to raise scattered the one-tenth of poultry Motility rate is promoted to more than 82% from 70%, and effect is clearly.Fully demonstrate this mixture and can obtain good enhancing poultry Immunity, the effect of defending and fighting against diseases.1, compared to brown sugar activation or cultivation, the clear bran of humidity 60% ~ 70% is not changing EM In the case of bacterium composition, significantly delay decaying of EM bacterium;2, add vegetalitas lactic acid bacteria at traditional E M strain to strengthen, improve Beneficial microbe retaining portion in poultry intestinal tract, thus improves feedstuff absorbance and the resistance against diseases of poultry;3, described Operational approach easy, with low cost.Raw materials used easily obtain in rural area.Can commonly use.
Below it is only embodiments of the invention, is not the restriction that the present invention is made any other form, for this area Technical staff for, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, made appoint What amendment, equivalent, improvement etc., should be included within the scope of the present invention.

Claims (5)

1. one kind utilizes vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute the method for antibiotic in feedstuff, it is characterised in that: institute The method of stating is to use spread cultivation vegetalitas lactic acid bacteria being added on of bean cake to use in the EM bacterium that spreads cultivation of clear bran, and both ratios are 1:10, Control humidity 60% ~ 70%, airtight save backup.
A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the side of antibiotic in feedstuff Method, it is characterised in that: described vegetalitas lactic acid bacteria culturers is lactobacillus germ, picogram lactobacillus probiotic bacteria-65, cheese Lactobacillus one or more mixture therein.
A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the side of antibiotic in feedstuff Method, it is characterised in that: after vegetalitas lactobacillus inoculation and bean cake powder are pressed the weight ratio mixing and stirring of 1:50, add water regulation Humidity is to 60% ~ 70%, and then under conditions of fully sealing, normal temperature fermentation completes spreading cultivation of plant lactobacillus thalline in more than 15 days.
A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the side of antibiotic in feedstuff Method, it is characterised in that: by EM strain after brown sugar water activates 2 ~ 3 hours, admix dry smart bran mixing according to weight ratio 1:100 After Jun Yun, the regulation mixture humidity that adds water is to 60 ~ 70%, and room temperature lower seal completes spreading cultivation of EM thalline after 72 ~ 80 hours.
5. utilize vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute antibiosis in feedstuff according to the described one of claim 1 or 2 The method of element, it is characterised in that: described plant lactobacillus strain can use the Semen Sojae Preparatum abrasive material completely that ferments to substitute.
CN201610570254.6A 2016-07-20 2016-07-20 A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the method for antibiotic in feedstuff Pending CN106173208A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1391823A (en) * 2001-06-14 2003-01-22 陈培生 Comprehensive processing method for thallus protein fodder
CN102742719A (en) * 2011-04-19 2012-10-24 上海新农饲料有限公司 Preparation method of probiotic solid-state fermented complete feed
CN103431197A (en) * 2013-08-27 2013-12-11 王辉 Method for preparing solid feed additive by transforming cordyceps sinensis fermented residue by using EM (Effective Microorganisms) flora
CN103815118A (en) * 2013-07-25 2014-05-28 天津金农脉生态科技有限公司 Biological feed based on traditional Chinese medicine residues and needle mushroom residues and preparation method of biological feed
CN104996721A (en) * 2015-07-29 2015-10-28 刘和辉 Production method and equipment of plant straw feed
CN105169116A (en) * 2015-08-13 2015-12-23 宿迁益客饲料有限公司 Feed additive for preventing and curing coccidiosis of ducks and preparation method and application thereof

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1391823A (en) * 2001-06-14 2003-01-22 陈培生 Comprehensive processing method for thallus protein fodder
CN102742719A (en) * 2011-04-19 2012-10-24 上海新农饲料有限公司 Preparation method of probiotic solid-state fermented complete feed
CN103815118A (en) * 2013-07-25 2014-05-28 天津金农脉生态科技有限公司 Biological feed based on traditional Chinese medicine residues and needle mushroom residues and preparation method of biological feed
CN103431197A (en) * 2013-08-27 2013-12-11 王辉 Method for preparing solid feed additive by transforming cordyceps sinensis fermented residue by using EM (Effective Microorganisms) flora
CN104996721A (en) * 2015-07-29 2015-10-28 刘和辉 Production method and equipment of plant straw feed
CN105169116A (en) * 2015-08-13 2015-12-23 宿迁益客饲料有限公司 Feed additive for preventing and curing coccidiosis of ducks and preparation method and application thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
张宗舟、柴强、赵紫平: "EM发酵饲料的优点", 《生物质资源再利用》 *
谭善杰: "植物性乳酸菌饲养肉鸭试验报告", 《山东畜牧兽医》 *

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