CN106173208A - A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the method for antibiotic in feedstuff - Google Patents
A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the method for antibiotic in feedstuff Download PDFInfo
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- CN106173208A CN106173208A CN201610570254.6A CN201610570254A CN106173208A CN 106173208 A CN106173208 A CN 106173208A CN 201610570254 A CN201610570254 A CN 201610570254A CN 106173208 A CN106173208 A CN 106173208A
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- lactic acid
- acid bacteria
- substitute
- vegetalitas
- bacterium
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- 241000894006 Bacteria Species 0.000 title claims abstract description 61
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 30
- 239000004310 lactic acid Substances 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000000855 fermentation Methods 0.000 title claims abstract description 15
- 230000004151 fermentation Effects 0.000 title claims abstract description 15
- 230000003115 biocidal effect Effects 0.000 title claims abstract description 14
- 239000000203 mixture Substances 0.000 claims abstract description 17
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 7
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 7
- 241000186660 Lactobacillus Species 0.000 claims description 12
- 229940039696 lactobacillus Drugs 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 7
- 230000033228 biological regulation Effects 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 4
- 241000196324 Embryophyta Species 0.000 claims description 4
- 239000003082 abrasive agent Substances 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 210000000582 semen Anatomy 0.000 claims description 3
- 230000007480 spreading Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 235000013351 cheese Nutrition 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 239000006041 probiotic Substances 0.000 claims description 2
- 230000000529 probiotic effect Effects 0.000 claims description 2
- 235000018291 probiotics Nutrition 0.000 claims description 2
- 244000144977 poultry Species 0.000 abstract description 14
- 239000000654 additive Substances 0.000 abstract description 8
- 230000000996 additive effect Effects 0.000 abstract description 7
- 230000036039 immunity Effects 0.000 abstract description 7
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 5
- 230000000968 intestinal effect Effects 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 3
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000002411 adverse Effects 0.000 abstract description 2
- 210000000941 bile Anatomy 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 238000009472 formulation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 7
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 5
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 241000411851 herbal medicine Species 0.000 description 3
- 238000002835 absorbance Methods 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- OMPJBNCRMGITSC-UHFFFAOYSA-N Benzoylperoxide Chemical compound C=1C=CC=CC=1C(=O)OOC(=O)C1=CC=CC=C1 OMPJBNCRMGITSC-UHFFFAOYSA-N 0.000 description 1
- 240000001929 Lactobacillus brevis Species 0.000 description 1
- 235000013957 Lactobacillus brevis Nutrition 0.000 description 1
- 244000199866 Lactobacillus casei Species 0.000 description 1
- 235000013958 Lactobacillus casei Nutrition 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 208000027954 Poultry disease Diseases 0.000 description 1
- 240000006677 Vicia faba Species 0.000 description 1
- 235000010749 Vicia faba Nutrition 0.000 description 1
- 235000002098 Vicia faba var. major Nutrition 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 235000019730 animal feed additive Nutrition 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000021107 fermented food Nutrition 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 239000008361 herbal raw material Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 229940017800 lactobacillus casei Drugs 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 235000021110 pickles Nutrition 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000021108 sauerkraut Nutrition 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 1
- 238000012549 training Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/179—Sakei
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fodder In General (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides and a kind of utilize vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute the method for antibiotic in feedstuff, belong to the production method of a kind of microorganism formulation, described method is, use the bean cake pulverized that vegetalitas lactic acid bacteria is spread cultivation, then according to the ratio of 1:10 mixes with the EM bacterium using clear bran to spread cultivation, keep humidity 60% ~ 70% to complete additive and prepare.Compared with the simple additive using EM bacterium, vegetalitas lactic acid bacteria is acidproof and bile tolerance ability is higher, and can survive under the adverse circumstances that nutrition is uneven, enters intestinal survival rate higher, and colonization ability is higher.It is remarkably improved beneficial microbe retaining portion in poultry intestinal tract, thus in terms of improving intestinal environment, raising immunity advantageously.
Description
Technical field
The invention belongs to animal feed additive field, a kind of substitute antibiotics strengthens the feedstuff of immunity of poultry
Addictive preparation method.
Background technology
Along with the progress of society with to healthy attention, green cultivation product is increasingly favored by consumers in general,
But traditional intensive culture easily causes the most popular of poultry disease, therefore typically all uses interpolation antibiosis in feedstuff
The mode of element prevents disease.The antibiotic content thus causing Fowl meat exceeds standard, and people knows from experience after eating the certain negative effect of generation.
In order to avoid the negative effect of antibiotic, the feed formula of domestic more existing producer research antibiotic-free.
Such as patent 201310167917 .6, a kind of Chinese medicine mixing chicken feed and preparation method thereof, proposition Chinese medicine with raise
The way of material mixed fermentation;Patent 201510077055.7, a kind of antibiotic-free chicken feed addictive and preparation method thereof, propose
Use Chinese herbal medicine is as additive, and gives the formula of Chinese herbal medicine;And for example patent 201610062450.2, a kind of antibiotic-free
The preparation method of type of fattening chicken feed propose use EM bacterium fermented feed, and add Mulberry bar, Fructus Momordicae, hundred Herba Pelargonii Graveolentiss fruits etc.
The method of Chinese herbal medicine substitute antibiotics.
Although more than invention can obtain positive effect, but is limited to source and the processing mode of herbal raw material, opposing
Easily there is fluctuation by a relatively large margin in the effect of disease, and processing process complex too increases cost, limiting it should
With universal.
EM bacterium is the various bacteria combination with good function researched and developed by Japanese Scientists.But micro-life of composition EM bacterium
Thing mostly is aerobic or facultative kind, therefore uses EM bacterium limited as the additive Use Limitation fruit improving immunity when simple.
Vegetalitas lactic acid bacteria is prevalent in the class in traditional vegetalitas fermented foods such as Pickles, sauerkraut, broad bean paste
Lactic acid bacteria, including Lactobacillus plantarum, lactobacillus casei, Lactobacillus brevis etc..Compared with animality lactic acid bacteria, vegetalitas lactic acid bacteria is resistance to
Acid and bile tolerance ability are higher, and can survive under the adverse circumstances that nutrition is uneven, enter intestinal survival rate higher, colonization ability
Higher.Therefore it is improving intestinal environment, improve in terms of immunity advantageously.Therefore the product having commercialization is used for improving people
Body intestinal health and raising immunity.
Experiment shows, vegetalitas lactic acid bacteria can form good symbiosis with the multiple beneficial microorganism in EM bacterium,
Strengthen and feed with resident in poultry intestinal tract of EM bacterium, and then strengthening poultry intestinal tract microorganism species is for the opposing of poor environment
Ability.Therefore, by correct combination, it is provided that the preparation side of a kind of simple and easy to do antibiotic-free livestock fodder additives
Method.
Summary of the invention
It is an object of the invention to provide one utilizes vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute antibiotic in feedstuff
Method.
The present invention is achieved through the following technical solutions:
A kind of utilizing vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute the method for antibiotic in feedstuff, described method is to use bean
Spread cultivation vegetalitas lactic acid bacteria being added on of the dregs of rice uses in the EM bacterium that spreads cultivation of clear bran, and both ratios are 1:10, control humidity 60% ~
70%, airtight save backup.
Preferably, described vegetalitas lactic acid bacteria culturers is lactobacillus germ, picogram lactobacillus probiotic bacteria-65, cheese
Lactobacillus one or more mixture therein.
Preferably, after vegetalitas lactobacillus inoculation and bean cake powder are pressed the weight ratio mixing and stirring of 1:50, add water tune
Joint humidity is to 60% ~ 70%, and then under conditions of fully sealing, normal temperature fermentation completes the expansion of plant lactobacillus thalline for more than 15 days
Training.
Preferably, by EM strain after brown sugar water activates 2 ~ 3 hours, admix dry smart bran according to weight ratio 1:100 and mix
After closing uniformly, the regulation mixture humidity that adds water is to 60 ~ 70%, and room temperature lower seal completes spreading cultivation of EM thalline after 72 ~ 80 hours.
Preferably, described plant lactobacillus strain can use the Semen Sojae Preparatum abrasive material completely that ferments to substitute.
The invention has the beneficial effects as follows: experiment shows, feed house using EM bacterium+vegetalitas mixture of lactic acid bacteria to add
Fowl, can make the survival rate raising poultry scattered be promoted to more than 82% from 70%, and effect is clearly.Fully demonstrating this mixture can
To obtain good enhancing immunity of poultry, the effect of defending and fighting against diseases.
1, compared to brown sugar activation or cultivate, the clear bran of humidity 60% ~ 70% do not change EM bacterium composition in the case of,
Significantly delay decaying of EM bacterium;
2, add vegetalitas lactic acid bacteria at traditional E M strain to strengthen, improve beneficial microbe retention in poultry intestinal tract
Ratio, thus improves feedstuff absorbance and the resistance against diseases of poultry;
3, described operational approach is easy, with low cost.Raw materials used easily obtain in rural area.Can commonly use.
Detailed description of the invention
A kind of utilize vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute the method for antibiotic in feedstuff, be by following step
Rapid realization:
A: tame the vegetalitas lactic acid bacteria that spreads cultivation.Bean cake powder is broken to powder, then accesses vegetalitas lactic acid bacteria culturers, by strain
With bean cake powder according to the weight ratio mixing and stirring of 1:50 after, add water regulation humidity to 60% ~ 70%, then fully sealing
Under conditions of normal temperature fermentation within 15 days, complete to spread cultivation, standby.
B: spread cultivation EM bacterium, after being activated 2 hours with brown sugar water by EM bacterium, admixes dry clear bran according to weight ratio 1:100
(clear bran is second time grinding product after Oryza sativa L. is shelled, and mainly contains substantial amounts of Oryza sativa L. plumule, seed coat and the mixing of rice flour
Thing) in, the regulation mixture humidity that adds water subsequently is to 60% ~ 70%, and room temperature lower seal completes to spread cultivation for 72 hours, standby.
C: take vegetalitas mixture of lactic acid bacteria 1 part prepared by step A and add EM bacterium prepared by step B and spread cultivation product 9 parts mixing,
Mix homogeneously, and regulate the humidity of mixture to 60% ~ 70%, prepared by the additive i.e. completing substitute antibiotics.This additive can
Guarantee the quality 3 months under normal temperature condition under air-proof condition.
In the present embodiment, thing lactic acid bacteria culturers can use the Semen Sojae Preparatum abrasive material completely that ferments to substitute.
During use, take additive prepared by preceding method according to the ratio of 2% and add in feedstuff, or 1% ratio by spice
Add in the drinking water of poultry, or both use simultaneously.
Experiment shows, is using EM bacterium+vegetalitas mixture of lactic acid bacteria to add feed for poultry, can make to raise scattered the one-tenth of poultry
Motility rate is promoted to more than 82% from 70%, and effect is clearly.Fully demonstrate this mixture and can obtain good enhancing poultry
Immunity, the effect of defending and fighting against diseases.1, compared to brown sugar activation or cultivation, the clear bran of humidity 60% ~ 70% is not changing EM
In the case of bacterium composition, significantly delay decaying of EM bacterium;2, add vegetalitas lactic acid bacteria at traditional E M strain to strengthen, improve
Beneficial microbe retaining portion in poultry intestinal tract, thus improves feedstuff absorbance and the resistance against diseases of poultry;3, described
Operational approach easy, with low cost.Raw materials used easily obtain in rural area.Can commonly use.
Below it is only embodiments of the invention, is not the restriction that the present invention is made any other form, for this area
Technical staff for, the present invention can have various modifications and variations.All within the spirit and principles in the present invention, made appoint
What amendment, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (5)
1. one kind utilizes vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute the method for antibiotic in feedstuff, it is characterised in that: institute
The method of stating is to use spread cultivation vegetalitas lactic acid bacteria being added on of bean cake to use in the EM bacterium that spreads cultivation of clear bran, and both ratios are 1:10,
Control humidity 60% ~ 70%, airtight save backup.
A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the side of antibiotic in feedstuff
Method, it is characterised in that: described vegetalitas lactic acid bacteria culturers is lactobacillus germ, picogram lactobacillus probiotic bacteria-65, cheese
Lactobacillus one or more mixture therein.
A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the side of antibiotic in feedstuff
Method, it is characterised in that: after vegetalitas lactobacillus inoculation and bean cake powder are pressed the weight ratio mixing and stirring of 1:50, add water regulation
Humidity is to 60% ~ 70%, and then under conditions of fully sealing, normal temperature fermentation completes spreading cultivation of plant lactobacillus thalline in more than 15 days.
A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the side of antibiotic in feedstuff
Method, it is characterised in that: by EM strain after brown sugar water activates 2 ~ 3 hours, admix dry smart bran mixing according to weight ratio 1:100
After Jun Yun, the regulation mixture humidity that adds water is to 60 ~ 70%, and room temperature lower seal completes spreading cultivation of EM thalline after 72 ~ 80 hours.
5. utilize vegetalitas lactic acid bacteria and EM bacterium mixed fermentation to substitute antibiosis in feedstuff according to the described one of claim 1 or 2
The method of element, it is characterised in that: described plant lactobacillus strain can use the Semen Sojae Preparatum abrasive material completely that ferments to substitute.
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CN201610570254.6A CN106173208A (en) | 2016-07-20 | 2016-07-20 | A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the method for antibiotic in feedstuff |
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CN201610570254.6A CN106173208A (en) | 2016-07-20 | 2016-07-20 | A kind of vegetalitas lactic acid bacteria and EM bacterium mixed fermentation is utilized to substitute the method for antibiotic in feedstuff |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1391823A (en) * | 2001-06-14 | 2003-01-22 | 陈培生 | Comprehensive processing method for thallus protein fodder |
CN102742719A (en) * | 2011-04-19 | 2012-10-24 | 上海新农饲料有限公司 | Preparation method of probiotic solid-state fermented complete feed |
CN103431197A (en) * | 2013-08-27 | 2013-12-11 | 王辉 | Method for preparing solid feed additive by transforming cordyceps sinensis fermented residue by using EM (Effective Microorganisms) flora |
CN103815118A (en) * | 2013-07-25 | 2014-05-28 | 天津金农脉生态科技有限公司 | Biological feed based on traditional Chinese medicine residues and needle mushroom residues and preparation method of biological feed |
CN104996721A (en) * | 2015-07-29 | 2015-10-28 | 刘和辉 | Production method and equipment of plant straw feed |
CN105169116A (en) * | 2015-08-13 | 2015-12-23 | 宿迁益客饲料有限公司 | Feed additive for preventing and curing coccidiosis of ducks and preparation method and application thereof |
-
2016
- 2016-07-20 CN CN201610570254.6A patent/CN106173208A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1391823A (en) * | 2001-06-14 | 2003-01-22 | 陈培生 | Comprehensive processing method for thallus protein fodder |
CN102742719A (en) * | 2011-04-19 | 2012-10-24 | 上海新农饲料有限公司 | Preparation method of probiotic solid-state fermented complete feed |
CN103815118A (en) * | 2013-07-25 | 2014-05-28 | 天津金农脉生态科技有限公司 | Biological feed based on traditional Chinese medicine residues and needle mushroom residues and preparation method of biological feed |
CN103431197A (en) * | 2013-08-27 | 2013-12-11 | 王辉 | Method for preparing solid feed additive by transforming cordyceps sinensis fermented residue by using EM (Effective Microorganisms) flora |
CN104996721A (en) * | 2015-07-29 | 2015-10-28 | 刘和辉 | Production method and equipment of plant straw feed |
CN105169116A (en) * | 2015-08-13 | 2015-12-23 | 宿迁益客饲料有限公司 | Feed additive for preventing and curing coccidiosis of ducks and preparation method and application thereof |
Non-Patent Citations (2)
Title |
---|
张宗舟、柴强、赵紫平: "EM发酵饲料的优点", 《生物质资源再利用》 * |
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