CN102232475A - Physiological regulator for male animals and preparation method thereof - Google Patents
Physiological regulator for male animals and preparation method thereof Download PDFInfo
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- CN102232475A CN102232475A CN2011100948621A CN201110094862A CN102232475A CN 102232475 A CN102232475 A CN 102232475A CN 2011100948621 A CN2011100948621 A CN 2011100948621A CN 201110094862 A CN201110094862 A CN 201110094862A CN 102232475 A CN102232475 A CN 102232475A
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Abstract
The invention discloses a physiological regulator for male animals and a preparation method thereof. The physiological regulator is characterized in that: the physiological regulator is a novel environment-friendly feed additive developed by combining Chinese medicines with a microbial fermentation technology. The technical scheme comprises the following steps of: crushing epimedium herb, eucommia, liquorice root, dried rehmannia root and the like, sieving, adding soybean meal, corn meal and/or bran, mixing, adding water and soaking, regulating the pH value, sterilizing, cooling, inoculating a probiotic and fermenting, and drying to obtain the physiological regulator after the fermentation is finished. The physiological regulator for the male animals can improve the immunity of the male animals, strengthen the reproductive function of the male animals, improve sperm activity and conception rate, remarkably prolong the mating time of the male animals and promote the mating quality of the male animals, quickly alleviate mating fatigue, and delay the senility of the male animals; the physiological regulator has no species specificity, has a steady and reliable effect, and is widely applicable to animals; the physiological regulator has the characteristics of no toxic or side effect, no pollution, no medicine residue and the like; and the physiological regulator guarantees the safety of animal products, is beneficial to the health of human bodies, and improves breeding economic benefit at the same time.
Description
Technical field
The present invention relates to a kind of buck with physiological regulation agent and preparation method thereof, belong to feed additive field.
Background technology
Along with the raising of people's living standard, people are growing to the demand of meat, eggs and milk, and this just requires to accelerate the development of livestock and poultry breeding industry.And breeding is the important step in the livestock and poultry breeding industry production, has only the fertility of raising just to accelerate and improve the quality.Therefore, how to keep buck physique solid, energetic, strengthen the function of its reproductive organs, make buck keep vigorous sexual desire, semen quality is good, improves the breeding conception rate, and delaying senility is the present producer and herding researcher urgent problem.Meanwhile, fully excavate the buck internal potential of growth fast, searching non-hormone, non-antibiotic class specificity buck growth promoter also are the pursuit of animal husbandry to the meat safety in production.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of have improve buck immunity, it is tired to alleviate breeding fast, the physiological regulation agent that strengthen breeding capacity, delays senility has instant effect, characteristics such as safe and reliable.
Another object of the present invention provides the preparation method of this buck with physiological regulation agent.
The present invention also provides a kind of feed that comprises this buck with physiological regulation agent.
To achieve these goals, the technical solution adopted in the present invention is as follows:
The invention provides a kind of buck and use physiological regulation agent, it is characterized in that probio is inoculated into to ferment in the culture medium that contains Chinese medicine being prepared from.
Described a kind of buck is used physiological regulation agent, it is characterized in that described Chinese medicine comprises following component and mass fraction:
1～10 part of barrenwort, 1～10 part of the bark of eucommia, 1～10 part in Radix Glycyrrhizae, 1～10 part of radix rehmanniae recen.
Described a kind of buck is used physiological regulation agent, it is characterized in that described probio comprises one or more of saccharomycete, lactic acid bacteria and/or gemma bacterium.
Described a kind of buck is used physiological regulation agent, it is characterized in that described Chinese medicine comprises that also mass fraction is one or more in the Poria cocos of 1～10 part dandelion, 1～5 part cynomorium songaricum, 1～10 part the Radix Astragali, 2～5 parts dried orange peel, 1～6 part the seed of Chinese dodder, 2～4 parts Radix Codonopsis, 1～5 portion of flos caryophylli, 1～4 part Morinda officinalis, 2～6 parts the bark of official magnolia, 1～5 part spina date seed, 1～10 part Chinese yam and 1～5 part.
Described a kind of buck is used physiological regulation agent, it is characterized in that described yeast is candida utili, saccharomyces cerevisiae and/or brewer's yeast.
Described a kind of buck is used physiological regulation agent, it is characterized in that described lactic acid bacteria comprises lactobacillus lactis, lactobacillus acidophilus, lactobacillus thermophilus, Lactobacillus casei, lactobacillus lactis, Lactobacillus plantarum and/or lactobacillus bulgaricus.
Described a kind of mother animal physiological regulation agent is characterized in that described gemma bacterium comprises bacillus subtilis and/or bacillus licheniformis.
Described a kind of buck is used the preparation method of physiological regulation agent, it is characterized in that may further comprise the steps:
(1) Chinese medicine preliminary treatment
Choose Chinese medicine, dry 2～3h down respectively at 50～60 ℃; Chinese medicine after the oven dry is pulverized, crossed 80～120 mesh sieves;
(2) preparation strain fermentating liquid
Each bacterial classification is inoculated in respectively in separately the optimum medium, cultivate primary seed solution under suitable condition, enlarge cultivation when each bacteria growing is transferred to respectively in the secondary seed fermentation tank during to exponential phase, stop when reaching exponential phase cultivating, standby;
(3) Chinese medicine fermentation
Chinese medicine behind the crushing screening is mixed according to a certain ratio, pack in the fermentation tank, adding accounts for the dregs of beans of traditional Chinese medicine quality 0～5%, 5～15% corn flour, 0～10% wheat bran, adds water to water content between 50～70%, behind immersion 12～24h, regulate between the pH value 6.0～7.5, at 121 ℃ of following steam sterilizing 15～20min, after the cooling, add 5%～15% the total strain liquid account for the fermentate gross mass, 28～37 ℃ fermented 24～72 hours, stirred once every 8h; After the fermentation ends,, promptly get buck and use physiological regulation agent 35～50 ℃ of oven dry down.
Described buck is with the preparation method of physiological regulation agent, it is characterized in that total strain liquid is the seed mixture liquid that the secondary seed solution by each bacterium is mixed and made into by equal proportion.
Described a kind of feed that contains buck with physiological regulation agent is characterized in that comprise the constituent of following mass ratio: described buck is used physiological regulation agent 0.02～0.2% and auxiliary material 99.98～99.8%.
The invention provides a kind of buck and use physiological regulation agent and preparation method thereof, have the following advantages:
1, buck of the present invention has the immunity that improves buck with physiological regulation agent, strengthen the reproduction of male animal function, improve spermatozoon activity, conception rate, obviously prolong its breeding time of lifting and quality, it is tired to alleviate fast breeding, and effect such as delay senility;
2, buck of the present invention is a kind of novel fodder additive of the interaction development of adopting probio and Chinese medicine with physiological regulation agent, have have no side effect, characteristics such as pollution-free, drug residue free; Ensure livestock products safety, be easy to people's health, increase the culturing economic benefit simultaneously;
3, buck of the present invention does not have the species specificity with physiological regulation agent, and effect stability is reliable, and it is wide to be suitable for animal range.
, it should be understood that described embodiment only for the present invention is described, rather than limit the scope of the invention by any way more specific description the present invention by following examples.
The specific embodiment
Embodiment 1
Chinese medicine: 6 parts of barrenwort, 8 parts in Radix Glycyrrhizae, 8 parts of the barks of eucommia, 5 parts of radix rehmanniae recens
The preparation method:
(1) S. cervisiae is inoculated in the malt extract medium, 28 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank that nutrient broth is housed with inoculum concentration 4%, cultivation temperature is 30 ℃, and rotating speed is 180rpm, when amount of yeast reaches 10
9During the CFU/ml left and right sides, stop fermentation, standby;
(2) choose above-mentioned each Chinese medicine, dry 2～3h down respectively at 50～60 ℃; Chinese medicine after the oven dry is pulverized, crossed the 80-120 mesh sieve, standby;
(3) take by weighing herb powder after the pulverizing by above-mentioned prescription, add and account for the dregs of beans of traditional Chinese medicine quality 1%, 5% corn flour, 5% wheat bran, add water to water content 60%, mix, at room temperature soak 24h, regulate about pH7.0, at 121 ℃ of following steam sterilizing 15min, after the cooling, add 5% the S. cervisiae bacterium liquid account for the fermentate gross mass, at 30 ℃ of fermentation 48h, stir once every 8h; When total viable count reaches 1.0 * 10
9Stop fermentation during CFU/ml, 45 ℃ of following vacuum drying make buck and use physiological regulation agent.
Embodiment 2
Chinese medicine: 5 parts of barrenwort, 3 parts in Radix Glycyrrhizae, 5 parts of the barks of eucommia, 2 parts of radix rehmanniae recens, 5 parts of the Radixs Astragali, 2 parts of cynomorium songaricums, 1 part of the seed of Chinese dodder
The preparation method:
(1) bacillus subtilis is inoculated in the common nutrient broth medium, at 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, enlarge in the secondary seed fermentation tank that primary seed solution is inoculated into inoculum concentration 3% and cultivate, cultivation temperature is 37 ℃, and rotating speed is 180rpm, when the gemma bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, standby;
(2) Lactobacillus plantarum is inoculated in the MRS culture medium, 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank that nutrient broth is housed with inoculum concentration 5%, cultivation temperature is 37 ℃, and rotating speed is 180rpm, when lactic acid bacteria content reaches 10
9During the CFU/ml left and right sides, stop fermentation, standby;
(3) choose above-mentioned each Chinese medicine, dry 2～3h down respectively at 50～60 ℃; Chinese medicine after the oven dry is pulverized, crossed the 80-120 mesh sieve, standby;
(4) take by weighing herb powder after the pulverizing by above-mentioned prescription, add and account for the dregs of beans of traditional Chinese medicine quality 3%, 8% corn flour, 4% wheat bran, add water to water content 55%, mix, at room temperature soak 24h, regulate about pH6.5, at 121 ℃ of following steam sterilizing 20min, after the cooling, adding accounts for the bacillus subtilis bacterium liquid of fermentate gross mass 4% and 4% Lactobacillus plantarum bacterium liquid respectively, at 37 ℃ of fermentation 36h, stir once every 8h; When total viable count reaches 1.0 * 10
9Stop fermentation during CFU/ml, 40 ℃ of following vacuum drying make buck and use physiological regulation agent.
Embodiment 3
Chinese medicine: 4 parts of barrenwort, 3 parts in Radix Glycyrrhizae, 6 parts of the barks of eucommia, 3 parts of radix rehmanniae recens, 2 parts of cynomorium songaricums, 1 part of the bark of official magnolia, 1 part of spina date seed, 2 parts of the Radixs Astragali
The preparation method:
(1) candida utili, brewer's yeast and saccharomyces cerevisiae are inoculated into respectively in the malt extract medium, 28～30 ℃, the 220rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, enlarge in the secondary seed fermentation tank that primary seed solution is inoculated into inoculum concentration 7% and cultivate, cultivation temperature is 28～30 ℃, and rotating speed is 220rpm, when amount of yeast reaches 10
9During the CFU/ml left and right sides, stop fermentation, standby;
(2) choose above-mentioned each Chinese medicine, dry 2～3h down respectively at 50～60 ℃; Chinese medicine after the oven dry is pulverized, crossed the 80-120 mesh sieve, standby;
(3) take by weighing herb powder after the pulverizing by above-mentioned prescription, add and account for the dregs of beans of traditional Chinese medicine quality 2%, 6% corn flour, 10% wheat bran, add water to water content 60%, mix, at room temperature soak 24h, regulate about pH6.9, at 121 ℃ of following steam sterilizing 20min, after the cooling, add the candida utili bacterium bacterium liquid account for fermentate gross mass 2%, 2% saccharomyces cerevisiae bacterium liquid and 2% S. cervisiae bacterium liquid respectively, at 28～30 ℃ of fermentation 48h, stir once every 8h; When total viable count reaches 1.0 * 10
9Stop fermentation during CFU/ml, 50 ℃ of following vacuum drying make buck and use physiological regulation agent.
Embodiment 4
Chinese medicine: 3 parts of barrenwort, 3 parts in Radix Glycyrrhizae, 6 parts of the barks of eucommia, 2 parts of dried orange peels, 1 part of the Radix Astragali, 1 part of Radix Codonopsis, 1 part of flos caryophylli, 2 parts of Chinese yams, 1 part of Morinda officinalis, 2 parts in Poria cocos
The preparation method:
(1) bacillus licheniformis and bacillus subtilis are inoculated into respectively in the common nutrient broth medium, at 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, enlarge in the secondary seed fermentation tank that primary seed solution is inoculated into inoculum concentration 3% and cultivate, cultivation temperature is 34.5 ℃, and rotating speed is 180rpm, when the gemma bacterial content reaches 10
9During the CFU/ml left and right sides, stop fermentation, standby;
(2) saccharomyces cerevisiae and product candida colliculosa are inoculated into respectively in the malt extract medium, 28 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, enlarge in the secondary seed fermentation tank that primary seed solution is inoculated into inoculum concentration 4.5% and cultivate, cultivation temperature is 34.5 ℃, and rotating speed is 180rpm, when amount of yeast reaches 10
9During the CFU/ml left and right sides, stop fermentation, standby;
(3) lactobacillus thermophilus and Lactobacillus casei are inoculated into respectively in the MRS culture medium, 37 ℃, the 180rpm shaking table is cultivated, and the production primary seed solution is when bacterial content reaches 10
7-8During the CFU/ml left and right sides, primary seed solution is inoculated into expansion cultivation in the secondary seed fermentation tank that nutrient broth is housed with inoculum concentration 4.5%, cultivation temperature is 34.5 ℃, and rotating speed is 180rpm, when lactic acid bacteria content reaches 10
9During the CFU/ml left and right sides, stop fermentation, standby;
(4) choose above-mentioned each Chinese medicine, dry 2～3h down respectively at 50～60 ℃; Chinese medicine after the oven dry is pulverized, crossed the 80-120 mesh sieve, standby;
(5) take by weighing herb powder after the pulverizing by above-mentioned prescription, add the dregs of beans that accounts for traditional Chinese medicine quality 4%, 10% corn flour, 5% wheat bran, add water to water content 52%, mix, at room temperature soak 24h, regulate about pH6.9, at 121 ℃ of following steam sterilizing 18min, after the cooling, add the bacillus licheniformis bacterium liquid that accounts for fermentate gross mass 2% respectively, 2% bacillus subtilis bacterium liquid, 2% candida utili bacterium bacterium liquid, 2% S. cervisiae bacterium liquid, 2% lactobacillus thermophilus bacterium liquid and 2% Lactobacillus casei bacterium liquid, at 34.5 ℃ of fermentation 36h, stir once every 8h; When total number of bacteria reaches 1.0 * 10
9Stop fermentation during CFU/ml, 50 ℃ of following vacuum drying make buck and use physiological regulation agent.
Embodiment 5
A kind ofly contain buck comprises following mass percent with the feed of physiological regulation agent (bull with) constituent: buck is with physiological regulation agent 0.1% and auxiliary material 99.9%.Promptly buck is 1000g with the addition of physiological regulation agent in 1 ton of feed.Feed can specifically comprise the constituent of following mass fraction: use 0.1 part of physiological regulation agent by the embodiment of the invention 2 prepared bucks, 60 parts of corns, 16.7 parts of wheat brans, 18 parts of big dregs of beans, 1.5 parts of stone flours, 1.2 parts of calcium monohydrogen phosphates, 1 part of salt, 0.5 part in sodium bicarbonate, 1 part of 1% breeding oxen premix.
Embodiment 6
A kind ofly contain buck comprises following mass percent with the feed of physiological regulation agent (boar with) constituent: buck is with physiological regulation agent 0.06% and auxiliary material 99.94%.Promptly buck is 600g with the addition of physiological regulation agent in 1 ton of feed.Feed can specifically comprise the constituent of following mass fraction: use 0.06 part of physiological regulation agent by the embodiment of the invention 3 prepared bucks, 65 parts of corns, 17.94 parts of wheat brans, 12 parts of big dregs of beans, 1 part of fish meal, 1.3 parts of stone flours, 1.3 parts of calcium monohydrogen phosphates, 0.4 part of salt, 1 part of 1% herd boar premix.
Below by concrete test example the beneficial effect of buck of the present invention with physiological regulation agent is described.
Test example 1
This test example has been studied with the buck of the embodiment of the invention 1 preparation with the influence of physiological regulation agent to the quantity and the quality of herd boar sperm.
Test method: select 10 of the public boars of the Du Luoke that is in a good state of health about 270 ages in days for use, be divided into two groups at random, control group fed does not add the basal diet of any medicine, and the test group buck of adding embodiment 1 preparation of feeding in basal diet per ton is used physiological regulation agent 500g; Fed 30 days continuously, semen collection in per 10 days is once measured unusual rate of semen volume, sperm concentration, sperm motility rate, acrosome and the rate of teratosperm of each boar.Result of the test sees Table 1.
The influence of table 1 pair herd boar sperm, sperm concentration, sperm motility rate, the unusual rate of acrosome and rate of teratosperm
*
*Letter
aExpression P＜0.01 is promptly compared with control group, and difference is extremely remarkable; Letter
bSignificant difference is promptly compared in expression P＜0.05 with control group.
Result of the test shows: the test group herd boar add buck with physiological regulation agent after ejaculate volume, the sperm concentration of the 20th day and the 30th day all significantly increase, sperm motility rate obviously improves, and compares with control group, difference is remarkable (P＜0.01) extremely.Test group add buck with physiological regulation agent after, the unusual rate of rate of teratosperm and acrosome all obviously reduces, and uses back the 20th day to compare significant difference (P＜0.05) with the 30th day rate of teratosperm with control group with the unusual rate of acrosome.Illustrate that buck of the present invention can increase the quantity of sperm and improve the quality of sperm with physiological regulation agent, thereby promote its fecundity.
Test example 2
This test example has been studied with the buck of the embodiment of the invention 4 preparations with the influence of physiological regulation agent to the quantity and the quality of breeding oxen sperm.
Test method: certain pasture provides 10 adult He Sitan breeding oxens that age in days is close, is divided into two groups at random, 5 every group, is respectively test group and control group.Test group is fed and is added the daily ration of the buck of the embodiment of the invention 4 preparations with physiological regulation agent, and addition is the 800g/ ton, the control group fed basal diet.Feed continuously after 30 days, volume of semen, sperm concentration, sperm motility and rate of teratosperm are measured in semen collection.
The result shows: compare with control group, the interpolation buck of feeding is not obvious with the breeding oxen volume of semen variation of physiological regulation agent, sperm concentration improves 19.05%, and (control group is 1.05 ± 0.12 hundred million/ml, test group is 1.25 ± 0.31 hundred million/ml), sperm motility improves 6.01% than control group, and (control group is 75.43%, test group is 79.96%), rate of teratosperm is than control group decline 30.88% (control group is 17.23%, and test group is 11.91%).This illustrates that buck of the present invention improves significantly with the reproduction function of physiological regulation agent to breeding oxen.
Test example 3
This test example is with the meat public chick test of AA of feeding of the buck physiological regulation agent of the embodiment of the invention 3 preparation
Choose the meat public chick of 40 plumage AA, (test group: feeding is added with the basal diet of the buck of the embodiment of the invention 3 preparations with physiological regulation agent, and addition is the 300g/ ton to be divided into two groups at random; The control group fed basal diet), every group 20 plumage.Feed after 30 days continuously, compare with control group, daily gain improves 12.1% (P＜0.01), and testosterone concentration improves 11.9% (P＜0.05) in the blood.This illustrates that buck of the present invention can promote the quick growth of public chick and strengthen the reproduction function with physiological regulation agent.
Claims (10)
1. a buck is used physiological regulation agent, it is characterized in that probio is inoculated into to ferment in the culture medium that includes Chinese medicine being prepared from.
2. a kind of buck according to claim 1 is used physiological regulation agent, it is characterized in that described Chinese medicine comprises following component and mass fraction:
1～10 part of barrenwort, 1～10 part of the bark of eucommia, 1～10 part in Radix Glycyrrhizae, 1～10 part of radix rehmanniae recen.
3. a kind of buck according to claim 1 is used physiological regulation agent, it is characterized in that described probio comprises one or more of saccharomycete, lactic acid bacteria and/or gemma bacterium.
4. a kind of buck according to claim 2 is used physiological regulation agent, it is characterized in that described Chinese medicine comprises that also mass fraction is one or more in the Poria cocos of 1～10 part dandelion, 1～5 part cynomorium songaricum, 1～10 part the Radix Astragali, 2～5 parts dried orange peel, 1～6 part the seed of Chinese dodder, 2～4 parts Radix Codonopsis, 1～5 portion of flos caryophylli, 1～4 part Morinda officinalis, 2～6 parts the bark of official magnolia, 1～5 part spina date seed, 1～10 part Chinese yam and 1～5 part.
5. a kind of buck according to claim 3 is used physiological regulation agent, it is characterized in that described yeast is candida utili, saccharomyces cerevisiae and/or brewer's yeast.
6. a kind of buck according to claim 3 is used physiological regulation agent, it is characterized in that described lactic acid bacteria comprises lactobacillus lactis, lactobacillus acidophilus, lactobacillus thermophilus, Lactobacillus casei, lactobacillus lactis, Lactobacillus plantarum and/or lactobacillus bulgaricus.
7. a kind of mother animal physiological regulation agent according to claim 3 is characterized in that described gemma bacterium comprises bacillus subtilis and/or bacillus licheniformis.
8. according to the preparation method of each described a kind of buck of claim 1～7, it is characterized in that may further comprise the steps with physiological regulation agent:
(1) Chinese medicine preliminary treatment
Choose Chinese medicine, dry 2～3h down respectively at 50～60 ℃; Chinese medicine after the oven dry is pulverized, crossed 80～120 mesh sieves;
(2) preparation strain fermentating liquid
Each bacterial classification is inoculated in respectively in separately the optimum medium, cultivate primary seed solution under suitable condition, enlarge cultivation when each bacteria growing is transferred to respectively in the secondary seed fermentation tank during to exponential phase, stop when reaching exponential phase cultivating, standby;
(3) Chinese medicine fermentation
Chinese medicine behind the crushing screening is mixed according to a certain ratio, pack in the fermentation tank, adding accounts for the dregs of beans of traditional Chinese medicine quality 0～5%, 5～15% corn flour, 0～10% wheat bran, adds water to water content between 50～70%, behind immersion 12～24h, regulate between the pH value 6.0～7.5, at 121 ℃ of following steam sterilizing 15～20min, after the cooling, add 5%～15% the total strain liquid account for the fermentate gross mass, 28～37 ℃ fermented 24～72 hours, stirred once every 8h; After the fermentation ends,, promptly get buck and use physiological regulation agent 35～50 ℃ of oven dry down.
9. buck according to claim 8 is with the preparation method of physiological regulation agent, it is characterized in that total strain liquid is the seed mixture liquid that the secondary seed solution by each bacterium is mixed and made into by equal proportion.
10. one kind comprises each described feed that contains buck with physiological regulation agent of claim 1～7, it is characterized in that, comprise the constituent of following mass ratio: described buck is used physiological regulation agent 0.02～0.2% and auxiliary material 99.98～99.8%.
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| CN103053859A (en) * | 2013-01-31 | 2013-04-24 | 曾龙 | Compound Chinese medicinal herb premix for breeding boars |
| CN104877921A (en) * | 2015-05-27 | 2015-09-02 | 广州环亚化妆品科技有限公司 | Saccharomycetes culture medium and preparation method thereof |
| CN105214074A (en) * | 2015-09-30 | 2016-01-06 | 孟令刚 | A kind of Orally administered composition comprising Herba Cynomorii and ferment |
| CN105285391A (en) * | 2015-10-21 | 2016-02-03 | 黑龙江省农业科学院畜牧研究所 | Feed additive capable of improving breeding capability of breeding pigs of Min pigs as well as preparation method and application of feed additive |
| CN105707439A (en) * | 2016-03-17 | 2016-06-29 | 中国科学院合肥物质科学研究院 | Preparation method of animal health-care nutrient solution rich in probiotics |
| CN106562036A (en) * | 2015-12-24 | 2017-04-19 | 陕西正能农牧科技有限责任公司西安高陵分公司 | Boar feed and preparation method thereof |
| CN107467385A (en) * | 2017-08-10 | 2017-12-15 | 广州广力莱生物科技有限公司 | A kind of herd boar mixed feed and preparation method thereof |
| CN108719591A (en) * | 2017-04-16 | 2018-11-02 | 韦智 | A kind of feed formula that can improve animal sperm active function |
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| CN103005177A (en) * | 2012-05-18 | 2013-04-03 | 句容市白兔镇老兵禽畜养殖专业合作社 | Breeding boar feed additive |
| CN103053859A (en) * | 2013-01-31 | 2013-04-24 | 曾龙 | Compound Chinese medicinal herb premix for breeding boars |
| CN104877921A (en) * | 2015-05-27 | 2015-09-02 | 广州环亚化妆品科技有限公司 | Saccharomycetes culture medium and preparation method thereof |
| CN105214074A (en) * | 2015-09-30 | 2016-01-06 | 孟令刚 | A kind of Orally administered composition comprising Herba Cynomorii and ferment |
| CN105285391A (en) * | 2015-10-21 | 2016-02-03 | 黑龙江省农业科学院畜牧研究所 | Feed additive capable of improving breeding capability of breeding pigs of Min pigs as well as preparation method and application of feed additive |
| CN106562036B (en) * | 2015-12-24 | 2020-08-14 | 陕西正能农牧科技有限责任公司 | Boar feed and preparation method thereof |
| CN106562036A (en) * | 2015-12-24 | 2017-04-19 | 陕西正能农牧科技有限责任公司西安高陵分公司 | Boar feed and preparation method thereof |
| CN105707439A (en) * | 2016-03-17 | 2016-06-29 | 中国科学院合肥物质科学研究院 | Preparation method of animal health-care nutrient solution rich in probiotics |
| CN108719591A (en) * | 2017-04-16 | 2018-11-02 | 韦智 | A kind of feed formula that can improve animal sperm active function |
| CN107467385A (en) * | 2017-08-10 | 2017-12-15 | 广州广力莱生物科技有限公司 | A kind of herd boar mixed feed and preparation method thereof |
| CN109619296A (en) * | 2019-01-28 | 2019-04-16 | 天津市畜牧兽医研究所 | Application of the grains dedicated fine fodder in terms of promoting the bull calf growth and development of He Sitan kind |
| CN109619295A (en) * | 2019-01-28 | 2019-04-16 | 天津市畜牧兽医研究所 | A kind of grains dedicated fine fodder of Holstein bull and application |
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