CN116391796A - Method for processing wangcao - Google Patents

Method for processing wangcao Download PDF

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Publication number
CN116391796A
CN116391796A CN202310088063.6A CN202310088063A CN116391796A CN 116391796 A CN116391796 A CN 116391796A CN 202310088063 A CN202310088063 A CN 202310088063A CN 116391796 A CN116391796 A CN 116391796A
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fermentation
parts
feed
wangcao
enzyme
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曾俊
黄世洋
梁琼
李叶红
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Guangxi University
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Guangxi University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/33Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/10Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/30Feeding-stuffs specially adapted for particular animals for swines
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention provides a method for processing wangcao, belonging to the field of feed preparation. The method for processing the wang grass comprises the steps of crushing the wang grass by a crusher, pressing water by a water pressing machine, grinding into powder, then mixing bacteria and enzymes to cooperatively ferment the composition, and finally carrying out multi-step fermentation and the like. The bacterial enzyme synergistic fermentation composition comprises the following components: 0.2-0.7 part of mixed bacterial agent, 0.4-0.7 part of compound enzyme, 7-13 parts of corn fiber, 3-7 parts of cane molasses, 8-14 parts of salicornia sinensis and 4-11 parts of peanut bran. The feed disclosed by the invention takes the wangcao as a main raw material, and has a good price advantage; the feed provided by the invention can provide sufficient protein and multiple nutrient elements for the cultivated body, and can accelerate the weight gain rate of the cultivated body so as to furthest promote the income of a cultivation farm.

Description

Method for processing wangcao
The application is a divisional application of patent application named as 'a method for processing wangcao and a bacterial enzyme synergistic fermentation composition', the application date of the original application is 2020, 03 and 30, and the application number is 202010238051.3.
Technical Field
The invention relates to the technical field of feed preparation, in particular to a method for processing wangcao.
Background
The pennisetum hydridum is a perennial plant of pennisetum genus of Gramineae family, and is called as pennisetum hydridum because its stem is similar to bamboo. The plant height is 2.5-3.5 m, the plant has 12-16 knots, the stem is 2-4 cm thick, the leaf length is about 160 cm, and the leaf width is 3-6 cm. The stem and leaf after drying contains crude protein 14.33%, crude fat 3.33%, crude fiber 33.64%, nitrogen-free extract 33.85%, and each hectare corresponds to the protein content of 8-10 hectare corn, so the stem and leaf is nutritious. The leaves of the bamboo-wood composite plant are soft, crisp and tender, have good palatability, can replace bamboo-wood frames, and can grow well in the south of China, in the Henan, in the Hebei and other places. Especially, the root system of the product is developed, the root length can reach more than 3 meters, a fibrous root network can be formed in a short time, moisture and soil can be firmly locked, and the product is an ideal plant for preventing water and soil loss and treating the barren beach steep slope and river levee.
The pennisetum hydridum is used as a high-quality pasture, contains a plurality of necessary nutrients required by livestock and poultry, and can be used with little or no additive so as to save cost. The pennisetum hydridum is not easy to age, not easy to be fibrillated and high in yield, so that grains can be greatly saved and the cultivation cost can be reduced when the pennisetum hydridum is used for cultivating the pennisetum hydridum, and the pennisetum hydridum has wide prospect in the field of pasture and livestock cultivation.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a method for processing wangcao.
In order to achieve the above object, the present invention provides the following solutions:
a method for processing wangcao, comprising the following steps:
s1: harvesting the prince grass mechanically, crushing the prince grass by a crusher, then introducing a water press to press out 35-55% of water, introducing a pulverizer, and sieving to obtain powder; sieving to obtain powder with particle size of 1-5 mm;
s2: placing the powder into a stirrer, adding the bacteria and enzymes to cooperatively ferment the composition, and mixing to obtain a mixture;
s3: placing the obtained mixture into a fermentation tank for fermentation, keeping the height of the mixture at 50-80cm in the tank body for normal fermentation, automatically turning the stack in the fermentation tank at the temperature of the mixture being higher than 40 ℃ during normal fermentation, ventilating and cooling, performing normal fermentation for 24-30h, performing anaerobic fermentation for 18-30h, and performing secondary normal fermentation for 24-48h after anaerobic fermentation; finally granulating to obtain a finished feed product;
the synergistic fermentation composition of the bacterial enzymes takes 100 parts of added weight per 100 parts of fermented wangcao as a unit, and comprises the following components: 0.5 part of mixed microbial inoculum, 0.6 part of compound enzyme, 10 parts of corn fiber, 5 parts of cane molasses, 10 parts of sargentgloryvine stem and 7 parts of peanut bran; the mixed microbial inoculum is prepared by mixing lactobacillus acidophilus, bacillus amyloliquefaciens, bacillus subtilis and saccharomycetes according to the mass ratio of 1.7-2.5:0.5-1.3:1.8-2.1:2-3.5; the complex enzyme is prepared by mixing phytase, mannanase, protease, cellulase and saccharifying enzyme according to the mass ratio of 1-2:0.1-0.4:1-1.7:3-5:1.9-2.6;
in the step S3, anaerobic fermentation is carried out for 26 hours at the temperature of 27-38 ℃ and secondary normal fermentation is carried out for 36 hours.
Preferably, the mixed microbial inoculum is prepared by mixing lactobacillus acidophilus, bacillus amyloliquefaciens, bacillus subtilis and saccharomycetes according to a mass ratio of 2.1:0.9:2:2.8.
The beneficial effects of the invention are as follows:
1. as can be seen from Table 1, the feed prepared in example 3 is the most preferred example. The daily average daily gain of the feed is improved by 6.6% compared with that of the feed of the comparative example 4, the feed conversion ratio is improved by 8.4%, the cost of the feed per unit weight is reduced by 5.6%, the secondary normal fermentation is favorable for the cellulose and lignin in the wang grass to be fully degraded, the microbial inoculum can be fully contacted with the wang grass fiber, the fermentation activity is promoted, the synthesis amount of mycoprotein and volatile fatty acid is increased, the nutritional value and the digestibility of the feed are improved, and the effect of the feed can be more obvious when the feed is used in large and medium pig farms. Example 1 has an 11.3% increase in daily average daily gain, a 12.5% increase in feed conversion ratio, and a 14.1% decrease in feed cost per unit weight gain over comparative example 5 using the prior art, and thus the feed prepared according to the present invention has a higher economic benefit.
2. Comparing the average daily gain of example 1 with that of comparative examples 1-3, it can be found that the effect value of the combination of the mixed microbial agent and the complex enzyme in the feed is improved by alpha= (88.7-68.4)/(88.7X100% = 22.9% compared with the sum of the effect values of the mixed microbial agent and the complex enzyme in the feed, respectively, which means that the effect value is improved by 22.9% in the case of the combination, which means that the mixed microbial agent and the complex enzyme produce corresponding synergistic effect.
3. The process method is simple and efficient, small in equipment occupation area, simple in equipment maintenance and suitable for popularization.
Detailed Description
The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
The terms "comprising" and "having" and any variations thereof herein are intended to cover a non-exclusive inclusion. For example, inclusion of a list of steps, processes, methods, etc. is not limited to the listed steps but may alternatively include steps not listed or may alternatively include other steps inherent to such processes, methods, products, or apparatus.
The raw materials and the auxiliary materials adopted by the invention can be purchased in the existing market or factories if no special description exists.
The invention will be described in further detail with reference to the following embodiments in order to make the objects, features and advantages of the invention more comprehensible.
Example 1
The bacterial enzyme synergistic fermentation composition comprises the following components in parts by weight, wherein the parts by weight are added for each 100 parts by weight of fermented wang grass: 0.2 part of mixed microbial inoculum, 0.4 part of compound enzyme, 7 parts of corn fiber, 3 parts of cane molasses, 8 parts of sargentgloryvine stem and 4 parts of peanut bran.
The mixed microbial inoculum is prepared by mixing lactobacillus acidophilus, bacillus amyloliquefaciens, bacillus subtilis and saccharomycetes according to the mass ratio of 1.7:0.5:1.8:2.
The complex enzyme is prepared by mixing phytase, mannanase, protease, cellulase and saccharifying enzyme according to the mass ratio of 1:0.1:1:3:1.9.
The method for processing the wangcao comprises the following steps:
s1: 100 parts of the royal jelly after mechanical harvesting are crushed by a crusher, water accounting for 35% of the water is pressed out by a water pressing machine, and then the crushed royal jelly is fed into a pulverizer, and powder with the particle size of 1mm is obtained through sieving in the step S1;
s2: placing the powder into a stirrer, adding the bacteria and enzyme synergistic fermentation composition, stirring and mixing for 5min to obtain a mixture;
s3: placing the obtained mixture into a fermentation tank for fermentation, keeping the height of the mixture at 50-80cm in the tank body for normal fermentation, automatically turning the stack in the fermentation tank when the temperature of the mixture is more than 40 ℃, ventilating and cooling, performing anaerobic fermentation when the fermentation is enough for 24 hours, performing fermentation for 18 hours at the temperature of 27 ℃, and performing secondary normal fermentation for 24 hours after anaerobic fermentation; finally granulating to obtain the finished feed product.
Example 2
The bacterial enzyme synergistic fermentation composition comprises the following components in parts by weight, wherein the parts by weight are added for each 100 parts by weight of fermented wang grass: 0.7 part of mixed microbial inoculum, 0.7 part of compound enzyme, 13 parts of corn fiber, 7 parts of cane molasses, 14 parts of sargentgloryvine stem and 11 parts of peanut bran.
The mixed microbial inoculum is prepared by mixing lactobacillus acidophilus, bacillus amyloliquefaciens, bacillus subtilis and saccharomycetes according to the mass ratio of 2.5:1.3:2.1:3.5.
The complex enzyme is prepared by mixing phytase, mannanase, protease, cellulase and saccharifying enzyme according to a mass ratio of 2:0.4:1.7:5:2.6.
The method for processing the wangcao comprises the following steps:
s1: 100 parts of the royal jelly after mechanical harvesting are crushed by a crusher, water with the concentration of 55% is pressed out by a water press, and then the crushed royal jelly is fed into a pulverizer, and powder with the particle size of 5mm is obtained through sieving in the step S1;
s2: placing the powder into a stirrer, adding the bacteria and enzyme synergistic fermentation composition, stirring and mixing for 5min to obtain a mixture;
s3: placing the obtained mixture into a fermentation tank for fermentation, keeping the height of the mixture at 80cm in the tank body for normal fermentation, automatically turning the stack in the fermentation tank when the temperature of the mixture is more than 40 ℃, ventilating and cooling, performing anaerobic fermentation when the fermentation is enough for 30 hours, performing fermentation for 30 hours at the temperature of 38 ℃, and performing secondary normal fermentation for 48 hours after anaerobic fermentation; finally granulating to obtain the finished feed product.
Example 3
The synergistic bacterial enzyme fermentation composition comprises the following components in parts by weight per 100 parts of fermented wangcao grass: 0.5 part of mixed microbial inoculum, 0.6 part of compound enzyme, 10 parts of corn fiber, 5 parts of cane molasses, 10 parts of sargentgloryvine stem and 7 parts of peanut bran.
The mixed microbial inoculum is prepared by mixing lactobacillus acidophilus, bacillus amyloliquefaciens, bacillus subtilis and saccharomycetes according to the mass ratio of 2.1:0.9:2:2.8.
The complex enzyme is prepared by mixing phytase, mannanase, protease, cellulase and saccharifying enzyme according to the mass ratio of 1.3:0.2:1.4:4.1:2.1.
The method for processing the wangcao comprises the following steps:
s1: 100 parts of the royal jelly after mechanical harvesting are crushed by a crusher, water with the concentration of 42% is pressed out by a water pressing machine, and then the crushed royal jelly is fed into a pulverizer, and powder with the particle size of 3mm is obtained through sieving in the step S1;
s2: placing the powder into a stirrer, adding the bacteria and enzyme synergistic fermentation composition, stirring and mixing for 5min to obtain a mixture;
s3: placing the obtained mixture into a fermentation tank for fermentation, keeping the height of the mixture at 65cm in the tank body for normal fermentation, automatically turning the stack in the fermentation tank when the temperature of the mixture is more than 40 ℃, ventilating and cooling, performing anaerobic fermentation when the fermentation is enough for 27 hours, performing fermentation for 26 hours at the temperature of 33 ℃, and performing secondary normal fermentation for 36 hours after anaerobic fermentation; finally granulating to obtain the finished feed product.
Comparative example 1
The method of working up the aquatics and the composition of the synergistic fermentation of the bacterial enzymes are substantially the same as in example 3, except that no mixed inoculant is added to the composition of the synergistic fermentation of the bacterial enzymes.
Comparative example 2
The method of working up the aquatics and the composition of the synergistic fermentation of the bacterial enzymes are substantially the same as in example 3, except that no complex enzyme is added to the composition of the synergistic fermentation of the bacterial enzymes.
Comparative example 3
The method for processing the prince grass and the composition for the synergistic fermentation of the bacterial enzymes are basically the same as those in the example 3, except that the composition for the synergistic fermentation of the bacterial enzymes is not added with mixed microbial inoculum and complex enzyme.
Comparative example 4
The method of working up the princess and the composition of the synergistic fermentation of the bacterial enzymes were substantially the same as in example 3, except that no secondary normal fermentation was performed in the method of working up the princess.
Comparative example 5
Patent is used: the feed prepared in the example of pennisetum hydridum combined pig feed (CN 201510136900.3).
160 healthy pigs of 50kg were selected and randomly distributed into 8 groups, 20 pigs of each group were fed with the feeds of examples 1 to 3 and comparative examples 1 to 5, respectively, and the weight and feed amount of each pig before the start of the test, during the test and at the end of the test were made, and the whole test was continued for 50 days. Specifically, the results are shown in Table 1.
Table 1 cases of feeding pork pigs with each group of feeds
Figure SMS_1
As can be seen from Table 1, the feed prepared in example 3 is the most preferred example. The daily average daily gain of the feed is improved by 6.6% compared with that of the feed of the comparative example 4, the feed conversion ratio is improved by 8.4%, the cost of the feed per unit weight is reduced by 5.6%, the secondary normal fermentation is favorable for the cellulose and lignin in the wang grass to be fully degraded, the microbial inoculum can be fully contacted with the wang grass fiber, the fermentation activity is promoted, the synthesis amount of mycoprotein and volatile fatty acid is increased, the nutritional value and the digestibility of the feed are improved, and the effect of the feed can be more obvious when the feed is used in large and medium pig farms. Example 1 has an 11.3% increase in daily average daily gain, a 12.5% increase in feed conversion ratio, and a 14.1% decrease in feed cost per unit weight gain over comparative example 5 using the prior art, and thus the feed prepared according to the present invention has a higher economic benefit.
Comparing the average daily gain of example 1 with comparative examples 1-3, it was found that example 1 increased by 26.4g (772.5-746.1 =26.4) over comparative example 1; example 1 increased by 42g (772.5-730.5 =42) over comparative example 2; example 1 increased 88.7g (772.5-683.8 =88.7) over comparative example 3; wherein the sum of the data of example 1 minus the data of comparative examples 1, 2, respectively, is 68.4g (26.4+42=68.4), which is less than 88.7g (68.4 < 88.7) of example 1 reduced relative to comparative example 3 in the absence of the mixed microbial agent, complex enzyme; taking the numerical values of each group of the comparative examples 1 and 2 subtracted from the numerical values of the example 1 as the effect values, the effect values of the mixed microbial inoculum and the compound enzyme used in combination in the feed are improved by alpha= (88.7-68.4) and 68.4 multiplied by 100% = 29% > 10% compared with the sum of the effect values of the mixed microbial inoculum and the compound enzyme used in the feed respectively, namely, the effect values are improved by 29% in the case of combined use, which indicates that the mixed microbial inoculum and the compound enzyme generate corresponding synergistic effect, and the average daily gain is synergistically improved, and the average daily gain is probably:
lactobacillus acidophilus in the mixed microbial inoculum can secrete antibiotics, such as: acidophilic lactobacillus, acidophilic bacillus and lactic acid bacteria, and has antagonism to intestinal pathogenic bacteria of farm animal and raised resistance. The polypeptide substance produced by the bacillus amyloliquefaciens can enhance the activity of mannase, protease and saccharifying enzyme, can also enhance the immunity of organisms and the antioxidation function of intestinal mucosa, and has the inhibition effect on micrococcus luteus and escherichia coli. The bacillus subtilis can produce protease and cellulase, so that the actual effect of the two enzymes is enhanced, antibiotics such as phospholipids, aminosugars, peptides, lipopeptides and the like can be used as main antibacterial substances, and the disease resistance of the cultivated body is improved. The yeast is rich in various vitamins, minerals and enzymes, so that the nutritional value of the feed is improved, the fermenting aroma can increase the feed intake of the cultivated body, and meanwhile, the yeast has a certain detoxification function and can improve the immunity of the cultivated body, so that the quality of meat is improved. The complex enzyme has a plurality of excellent effects, and the phytase can promote the growth of lactobacillus acidophilus and improve the absorption of Ca < 2+ > by the culture body. The mannosides produced by decomposition of mannanase and mannanase can promote proliferation of Lactobacillus acidophilus, bacillus amyloliquefaciens, bacillus subtilis and yeast, promote secretion of insulin-like growth factor IGF-I, promote protein synthesis, and increase lean meat percentage of cultured body. The protease can hydrolyze peptide chains one by one from the free carboxyl end or the free amino end of the polypeptide to generate amino acids, promote the growth of fungi in the composite microbial inoculum, improve the digestibility of feed proteins, peptides and amino acids, and play an important role in the digestive system function of monogastric animals. The cellulase can greatly improve the neutral washing fiber digestion rate, the acid washing fiber digestion rate and the energy digestion rate of the culture body, thereby improving the weight gain rate of the culture body. The saccharifying enzyme can cut alpha-1.4 bond, alpha-1.6 bond and alpha-1.3 bond from the non-reducing end of starch molecule, and the hydrolysis product is glucose.
In the present specification, each embodiment is described in a progressive manner, and each embodiment is mainly described in a different point from other embodiments, and identical and similar parts between the embodiments are all enough to refer to each other.
The principles and embodiments of the present invention have been described herein with reference to specific examples, the description of which is intended only to assist in understanding the methods of the present invention and the core ideas thereof; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.

Claims (2)

1. A method for processing wangcao, comprising the steps of:
s1: harvesting the prince grass mechanically, crushing the prince grass by a crusher, then introducing a water press to press out 35-55% of water, introducing a pulverizer, and sieving to obtain powder; sieving to obtain powder with particle size of 1-5 mm;
s2: placing the powder into a stirrer, adding the bacteria and enzymes to cooperatively ferment the composition, and mixing to obtain a mixture;
s3: placing the obtained mixture into a fermentation tank for fermentation, keeping the height of the mixture at 50-80cm in the tank body for normal fermentation, automatically turning the stack in the fermentation tank at the temperature of the mixture being higher than 40 ℃ during normal fermentation, ventilating and cooling, performing normal fermentation for 24-30h, performing anaerobic fermentation for 18-30h, and performing secondary normal fermentation for 24-48h after anaerobic fermentation; finally granulating to obtain a finished feed product;
the synergistic fermentation composition of the bacterial enzymes takes 100 parts of added weight per 100 parts of fermented wangcao as a unit, and comprises the following components: 0.5 part of mixed microbial inoculum, 0.6 part of compound enzyme, 10 parts of corn fiber, 5 parts of cane molasses, 10 parts of sargentgloryvine stem and 7 parts of peanut bran; the mixed microbial inoculum is prepared by mixing lactobacillus acidophilus, bacillus amyloliquefaciens, bacillus subtilis and saccharomycetes according to the mass ratio of 1.7-2.5:0.5-1.3:1.8-2.1:2-3.5; the complex enzyme is prepared by mixing phytase, mannanase, protease, cellulase and saccharifying enzyme according to the mass ratio of 1-2:0.1-0.4:1-1.7:3-5:1.9-2.6;
in the step S3, anaerobic fermentation is carried out for 26 hours at the temperature of 27-38 ℃ and secondary normal fermentation is carried out for 36 hours.
2. The method for processing the wang grass according to claim 1, wherein the mixed microbial inoculum is prepared by mixing lactobacillus acidophilus, bacillus amyloliquefaciens, bacillus subtilis and saccharomycetes according to a mass ratio of 2.1:0.9:2:2.8.
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CN109601736A (en) * 2019-01-14 2019-04-12 武汉新华扬生物股份有限公司 A kind of bacterium enzyme collaboration solid state fermentation pannage and preparation method thereof

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