CN112335783B - Sweet sorghum and lemon leaf mixed silage and preparation method thereof - Google Patents

Sweet sorghum and lemon leaf mixed silage and preparation method thereof Download PDF

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CN112335783B
CN112335783B CN202011129767.6A CN202011129767A CN112335783B CN 112335783 B CN112335783 B CN 112335783B CN 202011129767 A CN202011129767 A CN 202011129767A CN 112335783 B CN112335783 B CN 112335783B
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silage
sweet sorghum
raw materials
bacterial liquid
lemon
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CN112335783A (en
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周万海
冯瑞章
魏琴
张萍
霍香颖
陈霞
樊颖
邓梦玲
刘雯雯
杨丽
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Yibin University
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
    • A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
    • A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
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Abstract

The invention discloses a sweet sorghum and lemon leaf mixed silage and a preparation method thereof, wherein the mixed silage is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; the silage raw materials comprise sweet sorghum straw and lemon leaf in a mass ratio of 75:25-95:5; the compound bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis fermentation liquid and enterococcus faecalis fermentation liquid; the cellulose composite enzyme comprises cellulase and hemicellulase with a mass ratio of 1:1-4:1. When in preparation, the cellulose complex enzyme is firstly mixed with silage raw materials uniformly, then the complex bacterial liquid is sprayed on the surface of the mixture, and finally the mixture is fermented for 55 to 65 days at room temperature in a dark place, thus obtaining the microbial inoculum. By adopting the technical scheme of the invention, the problem of low resource utilization rate can be solved while the nutritive value of the feed is improved, and a new road is opened up for feed supply.

Description

Sweet sorghum and lemon leaf mixed silage and preparation method thereof
Technical Field
The invention belongs to the technical field of feed preparation, and particularly relates to sweet sorghum and lemon leaf mixed silage and a preparation method thereof.
Background
Sweet sorghum stalks are rich in sugar, and besides being used as sugar materials and energy crop materials, sweet sorghum can also be used as pasture, hay, green fodder and silage, wherein silage is the most commonly used and most efficient utilization form. The sweet sorghum has the characteristics of high sugar content, capability of providing sufficient energy substrate for lactic acid bacteria for growth, high content of soluble carbohydrates, low buffering energy value, quick decrease of pH value and the like, and is favorable for silage production. However, sweet sorghum contains more tannins, has the effect of resisting nutritional factors, can inhibit the action of rumen microorganisms, influences rumen fermentation and feed degradation, and obviously reduces the plant feeding value if the content of tannins exceeds 2% of the DM content; if it exceeds 5%, palatability and digestibility are affected, and animal productivity is lowered. The sweet sorghum has longer nutritional growth period, few grains and higher water content, so that the sweet sorghum can not only lose nutritional ingredients but also cause environmental pollution when directly ensiled.
The lemon leaves are unique in fragrance and rich in natural citric acid and other functional active substances, but the single lemon for feeding is poor in She Shikou property, low in nutrition concentration and unstable in nutrition ingredients, and special treatment such as squeezing is easy to cause nutrient loss, and drying is labor-consuming and time-consuming, so that most of the remaining lemon is thrown away as waste, and resources are wasted.
At present, the scale of the breeding industry in China is expanded, the problems of forage feeding, seasonal forage shortage, regional forage shortage and the like are increasingly outstanding, and besides the crop straw, the economic crop byproducts, the tree leaves and other unconventional feed resources are also required to be developed and utilized. The prior research reports that the sweet sorghum is a silage raw material superior to corn, but the sweet sorghum has high water content and is easy to decay after independent silage; the large amount of lemon leaves produced by pruning and shaping each year have low water content, are rich in active substances such as citric acid and the like, and are not recycled. However, research on mixed silage of sweet sorghum and lemon leaves has not been reported yet.
Disclosure of Invention
Aiming at the prior art, the invention provides sweet sorghum and lemon leaf mixed silage and a preparation method thereof, so as to solve the problems of low utilization rate of lemon leaf resources and shortage of forage feed.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: providing a sweet sorghum and lemon leaf mixed silage which is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; the silage raw materials comprise sweet sorghum straw and lemon leaf in a mass ratio of 75:25-95:5; the composite bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, saccharomycete, bacillus subtilis and enterococcus faecalis in the composite bacterial liquid is 70-75:45-55:8-12:5-10; the cellulose composite enzyme comprises cellulase and hemicellulase in a mass ratio of 1:1-4:1.
On the basis of the technical scheme, the invention can be improved as follows.
Further, the mass ratio of sorghum straw to lemon leaf in the silage raw material is 85:15.
Further, the ratio of the number of the forming units of the lactobacillus, the saccharomycetes, the bacillus subtilis and the enterococcus faecalis in the mixed bacterial liquid is 72:50:10:8.
Further, the mass ratio of cellulase to hemicellulase in the cellulose complex enzyme was 2:1.
The sweet sorghum and lemon leaf mixed silage is prepared through the following steps:
s1: crushing sweet sorghum straw and lemon leaves into small sections with the length less than 2cm, and mixing according to a proportion to obtain silage raw materials;
s2: adding cellulose complex enzyme into silage raw materials according to the proportion of 5-8 mg/kg, and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 5-10 mL/kg;
s4: and (3) filling the mixture subjected to the S3 treatment into a vacuum packaging bag, vacuumizing, and fermenting at 20-25 ℃ for 55-65 days in a dark place to obtain the finished product.
The preparation method of the invention can be further improved based on the scheme.
Further, the amount of the cellulose complex enzyme added in S2 was 6mg/kg.
Further, the spraying amount of the composite bacterial liquid in the step S3 is 8mL/kg.
Further, the fermentation temperature in S4 was 22℃and the fermentation time was 60 days.
The beneficial effects of the invention are as follows:
1. the invention uses the mixture of sweet sorghum and citric acid as silage raw materials, which not only can avoid resource waste and environmental pollution, but also opens up a new way for the source of feed, can promote the supply of feed and can effectively solve the problem of feed shortage.
2. According to the invention, the lemon leaves are added into the silage raw material, and are rich in limonene and citric acid, and the limonene and the citric acid are fermented together with sweet sorghum, so that the pH value of the feed can be reduced, the enzyme activity can be improved, and the proteolytic absorption can be promoted; the final feed has effects of improving gastrointestinal microorganism, inhibiting harmful microorganism, and promoting beneficial microorganism; not only can stimulate appetite of ruminant animals, but also can improve feed intake and digestibility.
3. The feed formula comprises a compound bacterial liquid and cellulose compound enzyme. The composite bacterial liquid contains various beneficial bacterial groups, can accelerate the decomposition of sweet sorghum straw and lemon leaves, and forms a competition relationship with mixed bacteria such as mould, saprophyte and the like, thereby avoiding the propagation of the mixed bacteria and ensuring the smooth fermentation; the cellulose compound enzyme can decompose the fibers of the sweet sorghum straw and the lemon leaf, so that the sweet sorghum straw and the lemon leaf are converted into nutrient substances such as polysaccharide, the nutritional value of the final product is higher, the fiber content is reduced, the palatability of the final product is enhanced, and the feed intake is higher.
Detailed Description
The following describes the present invention in detail with reference to examples.
Example 1
A sweet sorghum and lemon leaf mixed silage is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; silage raw materials comprise sweet sorghum straw and lemon leaf in a mass ratio of 85:15; the composite bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, saccharomycete, bacillus subtilis and enterococcus faecalis in the composite bacterial liquid is 72:50:10:8; the cellulose complex enzyme comprises cellulase and hemicellulase with a mass ratio of 2:1.
The mixed silage in this example was prepared by the following steps:
s1: crushing sweet sorghum straw and lemon leaves into small sections with the length less than 2cm, and mixing the sorghum straw and the lemon leaves according to the mass ratio of 85:15 to obtain silage raw materials;
s2: adding cellulose complex enzyme into silage raw materials according to the proportion of 6mg/kg (6 mg cellulose complex enzyme is added into each kilogram of silage raw materials), and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 8mL/kg;
s4: and (3) filling the mixture subjected to the S3 treatment into a vacuum packaging bag, vacuumizing, and fermenting at 22 ℃ for 60 days in a dark place.
Example 2
A sweet sorghum and lemon leaf mixed silage is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; silage raw materials comprise sweet sorghum straw and lemon leaf in a mass ratio of 75:25; the composite bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, saccharomycete, bacillus subtilis and enterococcus faecalis in the composite bacterial liquid is 70:45:12:5; the cellulose complex enzyme comprises cellulase and hemicellulase in a mass ratio of 1:1.
The mixed silage in this example was prepared by the following steps:
s1: crushing sweet sorghum straw and lemon leaves into small sections with the length less than 2cm, and mixing the sorghum straw and the lemon leaves according to the mass ratio of 75:25 to obtain silage raw materials;
s2: adding cellulose complex enzyme into silage raw materials according to the proportion of 8mg/kg (8 mg cellulose complex enzyme is added into each kilogram of silage raw materials), and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 5mL/kg;
s4: and (3) filling the mixture subjected to the S3 treatment into a vacuum packaging bag, vacuumizing, and fermenting at 25 ℃ for 55 days in a dark place.
Example 3
A sweet sorghum and lemon leaf mixed silage is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; silage raw materials comprise sweet sorghum straw and lemon leaf in a mass ratio of 95:5; the composite bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, saccharomycete, bacillus subtilis and enterococcus faecalis in the composite bacterial liquid is 75:55:8:10; the cellulose complex enzyme comprises cellulase and hemicellulase in a mass ratio of 4:1.
The mixed silage in this example was prepared by the following steps:
s1: crushing sweet sorghum straw and lemon leaves into small sections with the length less than 2cm, and mixing the sorghum straw and the lemon leaves according to the mass ratio of 95:5 to obtain silage raw materials;
s2: adding cellulose complex enzyme into silage raw materials according to the proportion of 5mg/kg (5 mg cellulose complex enzyme is added into each kilogram of silage raw materials), and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 10mL/kg;
s4: and (3) filling the mixture subjected to the S3 treatment into a vacuum packaging bag, vacuumizing, and fermenting at 20 ℃ for 65 days in a dark place.
Comparative example 1
A sweet sorghum and lemon leaf mixed silage is prepared from silage raw materials and compound bacterial liquid; silage raw materials comprise sweet sorghum straw and lemon leaf in a mass ratio of 85:15; the composite bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis fermentation liquid and enterococcus faecalis fermentation liquid, and the number ratio of the lactobacillus to the saccharomycete to the bacillus subtilis to the enterococcus faecalis is 72:50:10:8.
The mixed silage in this example was prepared by the following steps:
s1: crushing sweet sorghum straw and lemon leaves into small sections with the length less than 2cm, and mixing the sorghum straw and the lemon leaves according to the mass ratio of 85:15 to obtain silage raw materials;
s2: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 8mL/kg;
s3: and (3) filling the mixture subjected to the S2 treatment into a vacuum packaging bag, vacuumizing, and fermenting at 22 ℃ for 60 days in a dark place.
Comparative example 2
A sweet sorghum and lemon leaf mixed silage is prepared from silage raw material and cellulose complex enzyme; silage raw materials comprise sweet sorghum straw and lemon leaf in a mass ratio of 85:15; the cellulose complex enzyme comprises cellulase and hemicellulase with a mass ratio of 2:1.
The mixed silage in this example was prepared by the following steps:
s1: crushing sweet sorghum straw and lemon leaves into small sections with the length less than 2cm, and mixing the sorghum straw and the lemon leaves according to the mass ratio of 85:15 to obtain silage raw materials;
s2: adding cellulose complex enzyme into silage raw materials according to the proportion of 6mg/kg (6 mg cellulose complex enzyme is added into each kilogram of silage raw materials), and uniformly mixing to obtain a mixture;
s3: and (3) filling the mixture subjected to the S2 treatment into a vacuum packaging bag, vacuumizing, and fermenting at 22 ℃ for 60 days in a dark place.
Comparative example 3
A silage is prepared from silage raw material, compound bacterial liquid and cellulose compound enzyme; silage raw material is sweet sorghum straw; the composite bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, saccharomycete, bacillus subtilis and enterococcus faecalis in the composite bacterial liquid is 72:50:10:8; the cellulose complex enzyme comprises cellulase and hemicellulase with a mass ratio of 2:1.
The mixed silage in this example was prepared by the following steps:
s1: crushing sweet sorghum straw into small sections with the length less than 2cm to obtain silage raw materials;
s2: adding cellulose complex enzyme into silage raw materials according to the proportion of 6mg/kg (6 mg cellulose complex enzyme is added into each kilogram of silage raw materials), and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 8mL/kg;
s4: and (3) filling the mixture subjected to the S3 treatment into a vacuum packaging bag, vacuumizing, and fermenting at 22 ℃ for 60 days in a dark place.
Comparative example 4
A silage is prepared from silage raw material, compound bacterial liquid and cellulose compound enzyme; silage raw material is lemon leaf; the composite bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, saccharomycete, bacillus subtilis and enterococcus faecalis in the composite bacterial liquid is 72:50:10:8; the cellulose complex enzyme comprises cellulase and hemicellulase with a mass ratio of 2:1.
The mixed silage in this example was prepared by the following steps:
s1: crushing lemon leaves into small sections with the length less than 2cm to obtain silage raw materials;
s2: adding cellulose complex enzyme into silage raw materials according to the proportion of 6mg/kg (6 mg cellulose complex enzyme is added into each kilogram of silage raw materials), and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 8mL/kg;
s4: and (3) filling the mixture subjected to the S3 treatment into a vacuum packaging bag, vacuumizing, and fermenting at 22 ℃ for 60 days in a dark place.
Analysis of results
1. Sensory evaluation
Sensory evaluation was performed according to the german agricultural association (DLG) silage sensory scoring criteria and scale, which are shown in table 1. Directly observing and recording the color and mildew of the mixed storage of each proportion after opening the bag, and evaluating the smell and structure of the silage through smell and touch.
TABLE 1 sensory evaluation criteria
2. Analysis of nutritional ingredients
The results of analysis of the dry matter DM, crude protein CP, neutral detergent fiber NDF, acid detergent fiber ADF, soluble carbohydrate WSC, crude fat EE, crude Ash Ash, ca, P and other nutrients and mineral element content in the mixed silage are shown in Table 2.
Table 2 nutritional ingredients (dry matter basis) of sweet sorghum and lemon leaf mixed silage
As can be seen from the table, the mixed silage with high nutritive value and good palatability can be obtained by adopting the formula and the method of the invention.
Compared with the example 1, the comparative example 1 has the advantages that no cellulose complex enzyme is added in the raw materials, the decomposition of the fibers in the sweet sorghum and the lemon leaves is limited in the fermentation process, and more crude fibers exist in the final product, so that the nutritional value of the product is low, the palatability is poor, and the product is not accepted by livestock.
Compared with the example 1, the comparative example 2 has the advantages that the composite bacterial liquid is not added in the raw materials, the decomposition of microorganisms is absent in the fermentation process, the fermentation effect is poor, and the nutrient substances in the sweet sorghum straw and the lemon leaf cannot be released, so that the content of the nutrient substances in the final product is low.
Compared with the example 1, the silage raw material is only sweet sorghum, the sweet sorghum contains more tannins, has the effect of resisting nutrition factors, can inhibit the action of microorganisms, affects fermentation and degradation, and has low nutrient content and poor palatability; moreover, the sweet sorghum has higher water content, and the independent silage is easy to cause mildew, so that the quality of the feed is seriously affected.
Compared with the example 1, the silage raw material only has lemon leaves, the nutrition concentration is low, the nutrition ingredients are unstable, the nutrition loss is easy to be caused by the independent fermentation, and the silage raw material only adopts the lemon leaves for fermentation, is influenced by the characteristics of the lemon leaves, has poor palatability and is not favored by livestock.
While specific embodiments of the invention have been described in detail in connection with the examples, it should not be construed as limiting the scope of protection of the patent. Various modifications and variations which may be made by those skilled in the art without the creative effort are within the scope of the patent described in the claims.

Claims (7)

1. A sweet sorghum and lemon leaf mixed silage, which is characterized in that: the mixed silage is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; the silage raw materials comprise sweet sorghum straw and lemon leaf in a mass ratio of 75:25-95:5; the composite bacterial liquid comprises a lactic acid bacteria fermentation liquid, a saccharomycete fermentation liquid, a bacillus subtilis fermentation liquid and an enterococcus faecalis fermentation liquid, wherein the number ratio of lactic acid bacteria to saccharomycetes to bacillus subtilis to enterococcus faecalis in the composite bacterial liquid is 70-75:45-55:8-12:5-10; the cellulose composite enzyme comprises cellulase and hemicellulase in a mass ratio of 1:1-4:1; the mixed silage is prepared through the following steps:
s1: crushing sweet sorghum straw and lemon leaves into small sections with the length less than 2cm, and mixing according to a proportion to obtain silage raw materials;
s2: adding cellulose complex enzyme into silage raw materials according to the proportion of 5-8 mg/kg, and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 5-10 mL/kg;
s4: and (3) filling the mixture subjected to the S3 treatment into a vacuum packaging bag, vacuumizing, and fermenting at 20-25 ℃ for 55-65 days in a dark place to obtain the modified starch.
2. The sweet sorghum and lemon leaf mixed silage according to claim 1 wherein: the mass ratio of sorghum straw to lemon leaf in the silage raw material is 85:15.
3. The sweet sorghum and lemon leaf mixed silage according to claim 1 wherein: the ratio of the formation unit numbers of the lactobacillus, the saccharomycetes, the bacillus subtilis and the enterococcus faecalis in the composite bacterial liquid is 72:50:10:8.
4. The sweet sorghum and lemon leaf mixed silage according to claim 1 wherein: the mass ratio of the cellulase to the hemicellulase in the cellulose complex enzyme is 2:1.
5. The sweet sorghum and lemon leaf mixed silage according to claim 1 wherein: the addition amount of the cellulose complex enzyme in S2 is 6mg/kg.
6. The sweet sorghum and lemon leaf mixed silage according to claim 1 wherein: and S3, spraying the composite bacterial liquid with the spraying amount of 8mL/kg.
7. The sweet sorghum and lemon leaf mixed silage according to claim 1 wherein: and S4, fermenting at 22 ℃ for 60 days.
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CN115039835B (en) * 2022-06-24 2023-09-08 山东农业大学 Mixed silage of sweet sorghum and sesbania as well as preparation method and application thereof
CN115462447A (en) * 2022-10-06 2022-12-13 中国科学院亚热带农业生态研究所 Method for preparing sorghum straw whole-plant corn mixed storage forage grass

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CN103494037B (en) * 2013-09-30 2014-12-10 山东省健牧生物药业有限公司 Fur-bearing animal feed additive
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