CN106071186B - The preparation method of the dedicated glycolysis fine fodder ensilage of one broad sow - Google Patents
The preparation method of the dedicated glycolysis fine fodder ensilage of one broad sow Download PDFInfo
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- CN106071186B CN106071186B CN201610398012.3A CN201610398012A CN106071186B CN 106071186 B CN106071186 B CN 106071186B CN 201610398012 A CN201610398012 A CN 201610398012A CN 106071186 B CN106071186 B CN 106071186B
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Abstract
The invention belongs to feed, enzyme preparation and technical field of microbial fermentation, and in particular to the preparation method of the dedicated glycolysis fine fodder ensilage of a broad sow.Fresh herbage or root tuber stem tuber are prescinded, are beaten, lactic acid bacteria is accessed, after natural enzymolysis and anaerobic fermentation, ensilage is made；By one of grouts, food processing byproduct, cereal or it is a variety of mixed with clean water, molasses syrup, access lactobacillus inoculation liquid, after natural enzymolysis and anaerobic fermentation, fermentation fine fodder wet basis be made；Oxygen consumption fermenting microbe liquid is accessed into fine fodder silage fermentation substrate after natural oxygen consumption fermentation, the dedicated glycolysis fine fodder ensilage of sow is made.The glycolysis fine fodder ensilage is rich in fermentable fiber element lactic acid, calcium lactate, small peptide, amino acid, vitamin and probiotics, have the characteristics that nutrition is high, easy to digest, palatability is good, milk yield of sow can be significantly improved, hence it is evident that increase feed intake, substantially reduce prevention of sow constipation.
Description
Technical field
The invention belongs to feed, enzyme preparation and technical field of microbial fermentation, and in particular to the dedicated glycolysis essence of a broad sow
Expect the preparation method of ensilage.
Background technique
China is populous nation and the first in the world pig raising big country.2015, the Animal Husbandry in China output value up to 3.5 trillion yuans,
Live pig delivers 6.43 hundred million for sale, wherein sow livestock on hand 45,000,000, and 54,870,000 tons of pork yield.According to Ministry of Agriculture's statistical data,
National commercial feed yield reaches 2.18 hundred million tons within 2015.Wherein, 1.79 hundred million tons of complete feed, 21,510,000 tons of concentrated feed, in advance
6,400,000 tons of mixing, including 89,740,000 tons of pig feed yield, 90,000,000 tons of fowl material yield, 53,000,000 tons of aquatic feeds, cattle and sheep feed
8500000 tons, 3,500,000 tons of feeds of rabbit racoon dog pet etc., more than 500 ten thousand tons of national sow feed.Expect the year two thousand twenty whole nation mixed feed
Yield is up to 2.2 hundred million tons or more, and wherein sow feed is up to more than 800 ten thousand tons or more.
It is well known that the quality of agent of feed for nursing sow quality not only directly affect the lactation amount of sow, weanling pig at
The weanling weight of motility rate and piglet, but also will affect normal heat, the breeding of sow after wean.However, due to sow feed
The reasons such as low, the fodder cellulose poor quality of content of cellulose, cause China's milking sow lactation amount commonly relatively low, prevention of sow constipation ten
Divide seriously, constrains the development of live pig industry.Therefore, content of cellulose and the quality for improving agent of feed for nursing sow are very crucial.
Ensilage is exactly lactobacillus-fermented feed.Natural ensiling be exactly utilize lactic acid bacteria present on natural plant into
Row fermentation, since the content of lactic acid bacteria on natural plant is few, only accounts for the 0.01-1% of total number of bacteria.So in fermentation process
In, lactic acid bacteria is difficult rapidly to form dominant microflora, cannot reduce pH value in a short time.Its result: first is that various bacteriums are all
Temperature is risen rapidly in growth and breeding, the prepared fermentation phase is caused to extend.Second is that ensilage is made because of fever in preparation fermentation process
At the loss of a large amount of nutritional ingredient and energy, also cause that smell is pungent, situation of palatability difference.Third is that mould during the fermentation
The mass propagation of bacterium and spoilage organisms causes ensilage locally mouldy and rotten, and especially top, bottom and edge go mouldy, rot
Situation is serious.Fourth is that due to the presence of a large amount of miscellaneous bacteria, when ensilage opens cellar, it is easy to form secondary fermentation, be cut in feeding
Kainogenesis mildew or mouldy in flakes on face, will cause when in bad order and thoroughly goes mouldy, rots.In the world generally using exogenous
Addition lactic acid bacteria, improve ensiling quality.
Ensilage has been widely applied in raising dairy cattle, using in the ascendant in sow raising.Feeding experiment shows:
The cellulose quality of ensiling is high, and especially neutral detergent fiber content is high, and milk cow and sow can be stimulated to search for food, slow down sow just
It is secret, the partial function with fermentable fiber element.Therefore, how to make good use of ensilage in sow raising and be one is worth grinding
The project studied carefully and inquired into.
In recent years, fermented feed has become the main of animal husbandry development in livestock product safety as green, environment-friendly feed
Under this overall background of problem, have become the hot spot of current Animal nutrition research.Feed fermentation can not only open up feed resource,
Feed palatability is improved, but also feed digestibility can be improved.It is worth mentioning that fermented feed is inhibiting digestion to inhale
Breeding and the growth aspect effect for receiving Escherichia coli, salmonella and some pathogenic bacterias and pathogen in road are obvious, therefore,
Fermented feed is in recent years also increasingly paid attention in countries in the world, and feed fermentation technology is in the ascendant.Such as: according to statistics, Denmark arrives
Fermented feed is being applied on 2011 existing 60% pig farms, this number is still constantly rising；The U.S. and Canada to use hair
Ferment feed is also in popularization energetically；Fermented feed had also obtained preferable development in China in recent years.
For a long time, it is attempted to fresh herbage or root tuber stem tuber and dregs of beans, bran grain slag, corn protein powder and cereal
It ferments together.But fresh herbage or root tuber stem tuber are particularly easy to generate nitrite, while being also very easy to corruption, in turn
Lead to failure of entirely fermenting.It is existing research shows that: fresh herbage or root tuber stem tuber are relatively suitble to independent ensiling fermentation, fresh to herd
Grass or root tuber stem tuber ferment together with dregs of beans, bran grain slag, corn protein powder and cereal and still have technical problem, there is not yet fresh
Herbage or root tuber stem tuber ferment together with grouts, food processing byproduct, cereal successfully report or technology.
Summary of the invention
Low, the fodder cellulose poor quality for the current sow feed content of cellulose in China and cause milk yield of sow relatively low
With constipation is extremely serious and fresh herbage or root tuber stem tuber ferment together with grouts, food processing byproduct, cereal and can generate
The problem of nitrite and corruption, it is an object of the invention to: provide it is a kind of rich in fermentable fiber element, it is lactic acid, calcium lactate, small
Peptide, amino acid, vitamin and probiotics have the characteristics that the abundant balance of amino acid, nutrition is high, easy to digest, palatability is good, can
The dedicated glycolysis fine fodder ensilage of sow for significantly improving milk yield of sow, obviously increasing feed intake, substantially reducing prevention of sow constipation.
The technical scheme adopted by the invention is that:
The preparation method of the dedicated glycolysis fine fodder ensilage of one broad sow, steps are as follows:
1., the preparation of ensilage:
Fresh herbage and/or root tuber stem tuber are prescinded respectively, are beaten, connects and is mixed into lactobacillus inoculation liquid and complex enzyme, filling pressure
In fact after horizental silo or tower, natural enzymolysis and anaerobic fermentation, ensilage is made；
2., fermentation fine fodder wet basis preparation:
By one of grouts, food processing byproduct, cereal or it is a variety of mixed with clean water, molasses syrup after, connect mixed
Enter lactobacillus inoculation liquid and complex enzyme, after natural enzymolysis and anaerobic fermentation, fermentation fine fodder wet basis is made；
3., the preparation of fine fodder silage fermentation substrate:
After ensilage, fermentation fine fodder wet basis and mountain flour are mixed, fine fodder silage fermentation substrate is made；
4., the preparation of the dedicated glycolysis fine fodder ensilage of sow:
Oxygen consumption fermenting microbe liquid is accessed into fine fodder silage fermentation substrate after natural oxygen consumption fermentation, the dedicated glycolysis of sow is made
The dedicated glycolysis fine fodder ensilage butt of sow is made after fine fodder ensilage wet basis, or drying.
Further, the fresh herbage and/or root tuber stem tuber are fresh herbage in Minister Agriculture of China's feedstuff catalogue
And/or root tuber stem tuber feed is one or more, be silage corn, Alfalfa, clover, water hyacinth, water duckweed, water peanut,
One of Grain Production of Amaranthus, sweet potato, carrot, cassava, sweet potato, pumpkin, potato are a variety of；
The grouts be Minister Agriculture of China's feedstuff catalogue in oil meals it is one or more, be dregs of beans, rapeseed dregs,
Cotton dregs, peanut meal, flaxseed meal, sunflower seed dregs, rice bran meal, corn germ cake or above-mentioned grouts dilated product in
It is one or more；
The food processing byproduct is one kind or more of food processing byproduct in Minister Agriculture of China's feedstuff catalogue
Kind, be wheat bran, rice bran, wheat gluten flour, corn protein powder, rice protein powder, DDGS, acid-sludge, spirit stillage, beer vinasse,
One of starch sugar residue is a variety of；
The cereal be Minister Agriculture of China's feedstuff catalogue in cereals product it is one or more, be corn, paddy,
Crack rice, wheat, flour, oat, barley, sorghum or above-mentioned cereal one of dilated product or a variety of；
The molasses syrup be Minister Agriculture of China's feedstuff catalogue in molasses syrup it is one or more, for sucrose slurry/
Powder, glucose syrup/powder, fructose syrup/powder, maltodextrin slurry/powder, fructose syrup/powder, malt syrup/powder, oligosaccharide syrup/powder, sugarcane
One of molasses, beet molasses, soy molasses are a variety of.
Preferably, the fresh herbage and/or root tuber stem tuber be silage corn, Alfalfa, sweet potato, carrot, in pumpkin
It is one or more；
The grouts are one in the dilated product of dregs of beans, cotton dregs, rice bran meal, corn germ cake or above-mentioned grouts
Kind is a variety of；
The food processing byproduct is one of wheat bran, rice bran, corn protein powder, DDGS or a variety of；
The cereal is corn, crack rice, wheat, oat, barley or above-mentioned cereal one of dilated product or more
Kind；
The molasses syrup is one of glucose syrup/powder, Sugar cane molasses, beet molasses or a variety of.
Further, step 1. in ensilage preparation condition it is as follows:
The fresh herbage or root tuber stem tuber water content are 30%-90%, and fineness is 0.01~100cm, lactobacillus inoculation liquid
Inoculum concentration 0.01%-20%, complex enzyme additive amount 0.0001%-2%, complex enzyme are made of the enzyme of following weight ratio: cellulose
Enzyme, zytase, pectase and mannase weight ratio be 100:1~500:1~500:1~300；
The above percentage is mass percent.
Preferably, step 1. in ensilage preparation condition it is as follows:
In 65%-75%, fineness is 1~2cm, lactobacillus inoculation liquid inoculum concentration for the fresh herbage or root tuber stem tuber water content
1%-3%, complex enzyme additive amount 0.1%-0.5%, complex enzyme are made of the enzyme of following weight ratio: cellulase, zytase,
The weight ratio of pectase and mannase is 100:20~30:20~30:10~20.
Further, step 2. in fermentation fine fodder wet basis preparation condition it is as follows:
Clean water, grouts, food processing byproduct, cereal, molasses syrup weight ratio be 100:1~1000:0~300:
0~100:0~100；
Lactobacillus inoculation liquid inoculum concentration 1%~30%；
Complex enzyme additive amount 0.001%~5%；
Fermentation temperature: 6~60 DEG C；
Starting fermentation pH: natural；
Fermented type: anaerobic fermentation；
Moisture content in medium: 15%~90%；
Fermentation time: 6~480 hours；
The above percentage is mass percent.
Preferably, step 2. in fermentation fine fodder wet basis preparation condition it is as follows:
Clean water, grouts, food processing byproduct, cereal, molasses syrup weight ratio be 100:50~60:20~30:
10~20:5~10；
Lactobacillus inoculation liquid inoculum concentration 4%~8%；
Complex enzyme additive amount 0.12%~0.6%；
Fermentation temperature: 24~28 DEG C；
Starting fermentation pH: natural；
Fermented type: anaerobic fermentation；
Moisture content in medium: 40%~60%；
Fermentation time: 48~72 hours.
Further, the weight ratio of the ensilage, fermentation fine fodder wet basis, oxygen consumption fermenting microbe liquid and mountain flour is
100:1~1000:1~100:0.1~10；
The aerobic fermentation strain liquid is made of barms liquid, bacillus subtilis bacterium solution and bacillus licheniformis liquid,
Wherein, the viable bacteria spore quantity ratio of barms liquid, bacillus subtilis bacterium solution and bacillus licheniformis liquid is 100:1~1000:1
~500；
The fermentation condition of step 4. is as follows:
Fermentation temperature: 26~32 DEG C；
Starting fermentation pH: natural；
Fermented type: oxygen consumption fermentation；
Moisture content in medium: 20~80%；
Fermentation time: 3~360 hours；
The above percentage is mass percent.
Preferably, the weight ratio of the ensilage, fermentation fine fodder wet basis, oxygen consumption fermenting microbe liquid and mountain flour is 100:
30~100:6~12:0.005~1
The aerobic fermentation strain liquid is made of barms liquid, bacillus subtilis bacterium solution and bacillus licheniformis liquid,
Wherein, barms, bacillus subtilis and the viable bacteria of bacillus licheniformis spore quantity ratio are 100:1~1000:1~500；
The fermentation condition of step 4. is as follows:
Fermentation temperature: 26~32 DEG C；
Starting fermentation pH: natural；
Fermented type: oxygen consumption fermentation；
Moisture content in medium: 50%~60%；
Fermentation time: 24~48 hours.
The beneficial effects of the present invention are: during the fermentation, fresh herbage or root tuber stem tuber will not only produce the present invention
Raw nitrite, nor corruption can occur.Meanwhile glycolysis fine fodder ensilage prepared by the present invention is rich in fermentable fiber
Plain lactic acid, calcium lactate, small peptide, amino acid, vitamin and probiotics have the characteristics that nutrition is high, easy to digest, palatability is good, energy
Significantly improve milk yield of sow, hence it is evident that increase feed intake, substantially reduce prevention of sow constipation.
Specific embodiment
Below with reference to embodiment, the present invention is described in detail.
Embodiment 1
The preparation method of the dedicated glycolysis fine fodder ensilage of one broad sow, steps are as follows:
1., the preparation of ensilage:
It is 40-50% that fresh alfalfa, which is dried to moisture content,.It is 40- by 500kg moisture content using crushing and beating machine
50% fresh alfalfa, which prescinds, is crushed to 1cm, and 500kg fresh alfalfa powder is made.The Hu for being 80-85% by 100kg moisture content
100kg carrot paste is made in radish crushing and beating.
500kg fresh alfalfa powder and 100kg carrot paste are mixed and made into the ensiling that 600kg moisture content is 75% or so
Raw material.It accesses 10kg lactobacillus inoculation liquid and is mixed into 60g complex enzyme (complex enzyme cellulase 40g, zytase 8g, pectase
8g and mannase 4g), filling is compacted in horizental silo, natural enzymolysis and after anaerobic fermentation 15 days, and 595kg ensiling is made and raises
Material.
2., fermentation fine fodder wet basis preparation:
After 80kg clean water, 85kg dregs of beans, 80kg corn protein powder and 20kg molasses syrup are mixed, 25kg lactic acid is accessed
Strain liquid and it is mixed into 80g complex enzyme (complex enzyme cellulase 50g, zytase 12g, pectase 12g and mannase
6g), 25-26 DEG C, fermentation pH it is natural under the conditions of, carry out anaerobic fermentation 48-60 hour, be made 278-282kg fermentation fine fodder
Wet basis；
3., the preparation of fine fodder silage fermentation substrate:
After 595kg ensilage, 278-282kg fermentation fine fodder wet basis and 2-3kg mountain flour are sufficiently mixed uniformly, it is made
873-877kg fine fodder silage fermentation substrate.
4., the preparation of the dedicated glycolysis fine fodder ensilage of sow:
In 873-877kg fine fodder silage fermentation substrate, after access 50kg oxygen consumption strain liquid ferments 48 hours, 915- is made
The dedicated glycolysis fine fodder ensilage wet basis of 920kg sow, or dry and the dedicated glycolysis fine fodder ensilage of 425-430kg sow is made
Butt.
Embodiment 2
The preparation method of the dedicated glycolysis fine fodder ensilage of one broad sow, steps are as follows:
1., the preparation of ensilage:
It is 65-75% that fresh Silage Corn Straw, which is dried to moisture content,.Using crushing and beating machine, by 1000kg moisture
Content prescinds for the fresh Silage Corn Straw of 65-75% and is crushed to 1cm, and the fresh Silage Corn Straw powder of 1000kg is made.
10kg lactobacillus inoculation liquid is accessed in the fresh Silage Corn Straw powder of 1000kg and is mixed into 30g complex enzyme (complex enzyme
Cellulase 20g, zytase 4g, pectase 4g and mannase 2g), filling is compacted in horizental silo, natural enzymolysis
After anaerobic fermentation 15 days, 995kg ensilage is made.
2., fermentation fine fodder wet basis preparation:
After 100kg clean water, 100kg dregs of beans, 50kg corn protein powder and 50kg rice bran, 20kg molasses syrup are mixed,
Access 30kg lactobacillus inoculation liquid and be mixed into 80g complex enzyme (complex enzyme cellulase 50g, zytase 12g, pectase 12g and
Mannase 6g), 25-26 DEG C, fermentation pH it is natural under the conditions of, carry out anaerobic fermentation 48-60 hour, obtained 340-
345kg fermentation fine fodder wet basis；
3., the preparation of fine fodder silage fermentation substrate:
After 995kg ensilage, 340-345kg fermentation fine fodder wet basis and 3-4kg mountain flour are sufficiently mixed uniformly, pH is adjusted
To 6.5-7.5,1335-1355kg fine fodder silage fermentation substrate is made.
4., the preparation of the dedicated glycolysis fine fodder ensilage of sow:
In 1335-1355kg fine fodder silage fermentation substrate, after access 80kg oxygen consumption strain liquid ferments 48 hours, it is made
The dedicated glycolysis fine fodder ensilage wet basis of 1405-1410kg sow, or dry and the dedicated glycolysis fine fodder blueness of 550-560kg sow is made
Store feed butt.
Embodiment 3
The preparation method of the dedicated glycolysis fine fodder ensilage of one broad sow, steps are as follows:
1., the preparation of ensilage:
It is 50-60% that Alfalfa, which is dried to moisture content,.It is 50- by 1000kg moisture content using crushing and beating machine
60% fresh Silage Corn Straw, which prescinds, is crushed to 1cm, and the fresh Silage Corn Straw powder of 1000kg is made.
20kg lactobacillus inoculation liquid is accessed in the fresh Silage Corn Straw powder of 1000kg, and it is (compound to be mixed into 30g complex enzyme
Enzyme cellulase 20g, zytase 4g, pectase 4g and mannase 2g), filling is compacted in horizental silo, natural enzyme
1110kg ensilage is made with after anaerobic fermentation 15 days in solution.
2., fermentation fine fodder wet basis preparation:
After 100kg clean water, 100kg dregs of beans, 50kgDDGS and 50kg wheat bran, 20kg molasses syrup are mixed, access
(complex enzyme cellulase 30g, zytase 8g, pectase 8g and sweet dew are poly- with 50g complex enzyme is mixed into for 20kg lactobacillus inoculation liquid
Carbohydrase 4g), 25-26 DEG C, fermentation pH it is natural under the conditions of, carry out anaerobic fermentation 48-60 hour, be made 330-335kg ferment
Fine fodder wet basis；
3., the preparation of fine fodder silage fermentation substrate:
After 1110kg ensilage, 330-335kg fermentation fine fodder wet basis and 2-3kg mountain flour are sufficiently mixed uniformly, adjust
1440-1445kg fine fodder silage fermentation substrate is made in pH to 6.5-7.5.
4., the preparation of the dedicated glycolysis fine fodder ensilage of sow:
In 1440-1445kg fine fodder silage fermentation substrate, after access 80kg oxygen consumption strain liquid ferments 48 hours, it is made
The dedicated glycolysis fine fodder ensilage wet basis of 1515-1520kg sow, or dry and the dedicated glycolysis fine fodder blueness of 545-550kg sow is made
Store feed butt.
Embodiment 4
The preparation method of the dedicated glycolysis fine fodder ensilage of one broad sow, steps are as follows:
1., the preparation of ensilage:
It is 70-80% that carrot, which is dried to moisture content,.It is 70- by 1000kg moisture content using crushing and beating machine
1000kg fresh carrot slurry is made in 80% carrot crushing and beating.
30kg lactobacillus inoculation liquid is accessed in 1000kg fresh carrot slurry, is mixed into 60g complex enzyme (cellulose in complex enzyme
Enzyme 40g, zytase 8g, pectase 8g and mannase 4g), filling is compacted in horizental silo, natural enzymolysis and anaerobism hair
After ferment 15 days, 1130kg ensilage is made.
2., fermentation fine fodder wet basis preparation:
After 500kg clean water, 500kg dregs of beans, 300kgDDGS and 200kg wheat bran, 100kg molasses are mixed, access
100kg lactobacillus inoculation liquid and be mixed into 300g complex enzyme (complex enzyme cellulase 200g, zytase 40g, pectase 40g and
Mannase 20g), 25-26 DEG C, fermentation pH it is natural under the conditions of, carry out anaerobic fermentation 48-60 hour, obtained 1680-
1685kg fermentation fine fodder wet basis；
3., the preparation of fine fodder silage fermentation substrate:
After 1130kg ensilage, 1680-1685kg fermentation fine fodder wet basis and 5-6kg mountain flour are sufficiently mixed uniformly, adjust
PH to 6.5-7.5 is saved, 2850-2860kg fine fodder silage fermentation substrate is made.
4., the preparation of the dedicated glycolysis fine fodder ensilage of sow:
In 2850-2860kg fine fodder silage fermentation substrate, after access 200kg oxygen consumption strain liquid ferments 48 hours, it is made
The dedicated glycolysis fine fodder ensilage wet basis of 2980-3010kg sow, or dry and the dedicated glycolysis fine fodder of 1145-1150kg sow is made
Ensilage butt.
Embodiment 5
The preparation method of the dedicated glycolysis fine fodder ensilage of one broad sow, steps are as follows:
It is 40-45% that fresh Grain Production of Amaranthus, which is dried to moisture content,.Using crushing and beating machine, it is by 1000kg moisture content
The fresh alfalfa of 40-50%, which prescinds, is crushed to 1cm, and the fresh grain amaranth powder of 1000kg is made.It is by 500kg moisture content
500kg sweet potato paste is made in the sweet potato crushing and beating of 80-85%.
1000kg fresh alfalfa powder and 500kg carrot paste are mixed and made into the blueness that 1500kg moisture content is 75% or so
Store raw material.It accesses 50kg lactobacillus inoculation liquid and is mixed into 60g complex enzyme (complex enzyme cellulase 40g, zytase 8g, pectin
Enzyme 8g and mannase 4g), filling is compacted in horizental silo, natural enzymolysis and after anaerobic fermentation 15 days, and it is green that 1590kg is made
Store feed.
2., fermentation fine fodder wet basis preparation:
400kg clean water, 500kg dregs of beans, 300kg corn protein powder and 200kg spirit stillage, 150kg molasses are mixed
Afterwards, it accesses 150kg lactobacillus inoculation liquid and is mixed into 300g complex enzyme (complex enzyme cellulase 200g, zytase 40g, pectin
Enzyme 40g and mannase 20g), 25-26 DEG C, fermentation pH it is natural under the conditions of, carry out anaerobic fermentation 48-60 hour, it is obtained
1690-1700kg fermentation fine fodder wet basis；
3., the preparation of fine fodder silage fermentation substrate:
After 1590kg ensilage, 1690-1700kg fermentation fine fodder wet basis and 5-6kg mountain flour are sufficiently mixed uniformly, adjust
PH to 6.5-7.5 is saved, 3265-3270kg fine fodder silage fermentation substrate is made.
4., the preparation of the dedicated glycolysis fine fodder ensilage of sow:
In 3265-3270kg fine fodder silage fermentation substrate, after the compound oxygen consumption strain liquid of access 150kg ferments 48 hours,
The dedicated glycolysis fine fodder ensilage wet basis of 3380-3390kg sow is made, or dries and the dedicated glycolysis of 1650-1680kg sow is made
Fine fodder ensilage butt.
One, Major Nutrient substance content in the dedicated glycolysis fine fodder ensilage of sow prepared by embodiment 1, is shown in Table 1.
Nutrition content in the dedicated glycolysis fine fodder silage production of sow prepared by table 1, embodiment 1
| Project | Index | Project | Index | Project | Index |
| Color | Yellowish-brown | Pfansteihl/% | ≥5.0 | Fermentable fiber/% | ≥1.5 |
| Moisture/% | ≤12.0 | L-calcium lactate/% | ≥0.5 | Total amount of probiotics cfu/g | ≥0.03×106 |
| Crude protein/% | ≥18.0 | Cellulose/% | ≥6.0 | Water-soluble substances | ≥20.0 |
Two, the dedicated glycolysis fine fodder ensilage of sow that prepared by embodiment 1 feeds milking sow effect.
For feeding effect of the dedicated glycolysis fine fodder ensilage of sow to milking sow for inquiring into the preparation of embodiment 1, selection
It grows up similar in blood relationship, parity, weight and birth dates lactating sow 120, the young number 10.5 of sow head band, sow head is
220 kilograms are weighed, 2 groups (control group and test groups) are randomly divided into.The dedicated glycolysis fine fodder ensiling of 8% sow is fed in test group material to raise
Expect (referred to herein as the dedicated glycolysis fine fodder ensilage of sow that mass fraction is 8% is added in basal diet), in control group
The basal diet of the dedicated glycolysis fine fodder ensilage of sow is not added.Control group is identical with test group basal diet.
Table 2, basal diet composition and trophic level
Test carries out 35 days (from farrowing to heat again), is raised by pig farm conventinal breeding mode.Wet-mixing material is free
Feeding, free water.On-test, every group of carry out individual weighing calculated bulk diffusion at the end of, counts feed intake and piglet increases
Weight situation.
As can be seen from Table 3, after the dedicated glycolysis fine fodder ensilage of 8% sow is added in basal diet, sow 8-28 days
Feed intake increased to 5.5kg/ days by 5.0kg/ days, increase by 10.0%, while also observing that the weight of sow does not have significantly
Loss.
As can be seen from Table 4, after the dedicated glycolysis fine fodder ensilage of 8% sow is added in basal diet, suckling pig is disconnected
10.45% is improved than control group average weight when milk；Sow body weight loss reduces by 33.3%；Heat number of days was reduced to by 6.5 days
6.0 days, shorten 0.5 day.
The influence of the dedicated glycolysis fine fodder ensilage of sow prepared by table 3, embodiment 1 to sow feed intake and weight
The influence of the dedicated glycolysis fine fodder ensilage of sow prepared by table 4, embodiment 1 to weaned piglets
| Group | Equal starting weight (kg) | Wean end weight (kg) | It increases weight (kg) | More weight gains (kg) | Disease incidence (%) |
| Control group | 1.35 | 6.7 | 5.35 | - | 8.3 |
| Test group | 1.3 | 7.4 | 6.1 | 0.75 | 1.2 |
Three, the dedicated glycolysis fine fodder ensilage feeding hybridization piglet effect of the sow that prepared by embodiment 1.
For feeding effect of the dedicated glycolysis fine fodder ensilage of sow to hybridization piglet for inquiring into the preparation of embodiment 1, choose
The Ternary Pig three way cross piglet 168 (59 and 60 age in days piglet) of selective body heavy phase nearly (18.9 ± 0.85kg of weight), at random
It is divided into test group and control group, test group 84 (sow 44, boar 40), control group 84 (sow 38, boar 46
Head).Two groups of pig weights are close (significance analysis is not significant), and each group is all provided with 7 repetitions, each repetition 12.
Test group is identical as control group piglet basal diet, is powder.Control group does not add the dedicated glycolysis fine fodder of sow
Ensilage, test group add the dedicated glycolysis fine fodder ensilage of 6% sow.Daily ration is uniformly mixed, and the coefficient of variation is small after measured
In 8%.Daily ration configure using G national standard GB/T5915 piglet, grow-finish swine feed standard as reference, composition and nutrition at
Part is shown in Table 5.The feedstuffs such as its corn, dregs of beans, wheat bran, imported fish meal, which meet national standard (GB13078-2001) quality through detection, to be wanted
It asks.
Table 5, test piglet basal diet composition and trophic level
| Raw material composition | Content/% | Nutrient | Content |
| Corn | 61.00 | Digestible energy DE | 12.35MJ·kg-1 |
| Dregs of beans | 25.00 | Dry matter DM | 87.74% (actual measurement) |
| Wheat bran | 6.00 | Crude fat EE | 3.52% |
| Imported fish meal | 3.00 | Thick protein CP | 17.02% |
| Oil powder | 1.00 | Crude fibre CF | 4.22% |
| Premix | 4.00 |
Note: contain mountain flour, calcium monohydrogen phosphate, salt, 20% lysine, vitamin and microelement in experimental period premix
Deng.
Since on January 7th, 2016 is formal, on March 1st, 2016 formally terminates for test, goes through examination 54 days.Test result is such as
Under:
As can be seen from Table 6, after the dedicated glycolysis fine fodder ensilage of 6% sow is added in daily ration, 42 piglets of test group
Average end weight 38.06kg, the average end weight 35.71kg of control group, test group have more 2.35kg than control group, improve 6.58% (P
<0.05)；Test group average weight gain 19.24kg, control group average weight gain 16.66kg, test group have more 2.58kg than control group,
Improve 15.49% (P < 0.01)；Test group average daily gain 356.30g, control group average daily gain 308.52g, test group
47.78g is had more than control group, improves 15.49% (P < 0.01).
The influence of the dedicated glycolysis fine fodder ensilage of sow prepared by table 6, embodiment 1 to piglet daily gain
Note: * * indicates to arrange the interior significance of difference (P < 0.01), the significance of difference (P < 0.05) in * expression column.Table 2 is herewith.
As can be seen from Table 7, after the dedicated glycolysis fine fodder ensilage of 6% sow is added in daily ration, test group every feeding
45.21kg, control group every feed intake 40.32kg, test group 40.89kg more than control group are measured, feed intake increases 12.13%
(P<0.05)；Test group feed conversion rate 2.35, than control group 2.42 few 0.07, test group reduces 2.89% (P than control group
>0.05)。
The influence of the dedicated glycolysis fine fodder ensilage of sow prepared by table 7, embodiment 1 to feed intake and feed conversion rate
| Group | Head number | Weight gain/head/kg | Feed intake/head/kg | Feed conversion rate | Feed cost/member |
| Control group | 84 | 16.66 | 40.32±1.23 | 2.42±0.49 | 60.48 |
| Test group | 84 | 19.24 | 45.21±2.35* | 2.35±0.83 | 67.82 |
Above content is only citing made for the present invention and explanation, affiliated those skilled in the art are to being retouched
The specific embodiment stated does various modifications or additions or is substituted in a similar manner, as long as it does not deviate from the concept of invention
Or beyond the scope defined by this claim, it is within the scope of protection of the invention.
Claims (8)
1. the preparation method of the dedicated glycolysis fine fodder ensilage of a broad sow, which is characterized in that steps are as follows:
1., the preparation of ensilage:
Fresh herbage and/or root tuber stem tuber are prescinded respectively, are beaten, connects and is mixed into lactobacillus inoculation liquid and complex enzyme, filling is compacted at
After horizental silo or tower, natural enzymolysis and anaerobic fermentation, ensilage is made；
2., fermentation fine fodder wet basis preparation:
It a variety of mixes one of grouts, the food processing byproduct in addition to grouts, cereal or with clean water, molasses syrup
Afterwards, it connects and is mixed into lactobacillus inoculation liquid and complex enzyme, after natural enzymolysis and anaerobic fermentation, fermentation fine fodder wet basis is made；
3., the preparation of fine fodder silage fermentation substrate:
After ensilage, fermentation fine fodder wet basis and mountain flour are mixed, fine fodder silage fermentation substrate is made；
4., the preparation of the dedicated glycolysis fine fodder ensilage of sow:
Aerobic fermentation strain liquid is accessed into fine fodder silage fermentation substrate after natural oxygen consumption fermentation, the dedicated glycolysis fine fodder of sow is made
The dedicated glycolysis fine fodder ensilage butt of sow is made after ensilage wet basis, or drying.
2. the preparation method of the dedicated glycolysis fine fodder ensilage of a broad sow according to claim 1, it is characterised in that:
The fresh herbage and/or root tuber stem tuber are fresh herbage and/or root tuber stem tuber in Minister Agriculture of China's feedstuff catalogue
Feed it is one or more, be silage corn, Alfalfa, clover, water hyacinth, water duckweed, water peanut, Grain Production of Amaranthus, carrot,
One of cassava, sweet potato, pumpkin, potato are a variety of；
The grouts are the one or more of oil meals in Minister Agriculture of China's feedstuff catalogue, are dregs of beans, rapeseed dregs, cotton
One of the dregs of rice, peanut meal, flaxseed meal, sunflower seed dregs, rice bran meal, corn germ cake or dilated product of above-mentioned grouts
Or it is a variety of；
The food processing byproduct in addition to grouts is the food processing in Minister Agriculture of China's feedstuff catalogue in addition to grouts
Byproduct it is one or more, be wheat bran, rice bran, wheat gluten flour, corn protein powder, rice protein powder, DDGS, white wine wine
One of grain, beer vinasse are a variety of；
The cereal is the one or more of cereals product in Minister Agriculture of China's feedstuff catalogue, is corn, paddy, broken
Rice, wheat, flour, oat, barley, sorghum or above-mentioned cereal one of dilated product or a variety of；
The molasses syrup is the one or more of molasses syrup in Minister Agriculture of China's feedstuff catalogue, is sucrose slurry/powder, Portugal
Grape syrup/powder, fructose syrup/powder, maltodextrin slurry/powder, malt syrup/powder, Sugar cane molasses, beet molasses, in soy molasses
It is one or more.
3. the preparation method of the dedicated glycolysis fine fodder ensilage of a broad sow according to claim 2, it is characterised in that:
The fresh herbage and/or root tuber stem tuber are one of silage corn, Alfalfa, sweet potato, carrot, pumpkin or more
Kind；
The grouts are one of dilated product of dregs of beans, cotton dregs, rice bran meal, corn germ cake or above-mentioned grouts or more
Kind；
The food processing byproduct in addition to grouts is one of wheat bran, rice bran, corn protein powder, DDGS or a variety of；
The cereal is corn, crack rice, wheat, oat, barley or above-mentioned cereal one of dilated product or a variety of；
The molasses syrup is one of glucose syrup/powder, Sugar cane molasses, beet molasses or a variety of.
4. the preparation method of the dedicated glycolysis fine fodder ensilage of a broad sow according to claim 1, it is characterised in that: step
Suddenly 1. the preparation condition of middle ensilage is as follows:
The fresh herbage or root tuber stem tuber water content are 30%-90%, and fineness is 0.01~100cm, the inoculation of lactobacillus inoculation liquid
0.01%-20%, complex enzyme additive amount 0.0001%-2% are measured, complex enzyme is made of the enzyme of following weight ratio: cellulase, wood
The weight ratio of dextranase, pectase and mannase is 100:1~500:1~500:1~300；
The above percentage is mass percent.
5. the preparation method of the dedicated glycolysis fine fodder ensilage of a broad sow according to claim 4, it is characterised in that: step
Suddenly 1. the preparation condition of middle ensilage is as follows:
In 65%-75%, fineness is 1~2cm, lactobacillus inoculation liquid inoculum concentration 1%- for the fresh herbage or root tuber stem tuber water content
3%, complex enzyme additive amount 0.1%-0.5%, complex enzyme is made of the enzyme of following weight ratio: cellulase, zytase, pectin
The weight ratio of enzyme and mannase is 100:20~30:20~30:10~20.
6. the preparation method of the dedicated glycolysis fine fodder ensilage of a broad sow according to claim 1, which is characterized in that step
Suddenly 2. the preparation condition of middle fermentation fine fodder wet basis is as follows:
Clean water, grouts, the food processing byproduct in addition to grouts, cereal, molasses syrup weight ratio be 100:50~60:20
~30:10~20:5~10；
Lactobacillus inoculation liquid inoculum concentration 4%~8%；
Complex enzyme additive amount 0.12%~0.6%；
Fermentation temperature: 24~28 DEG C；
Starting fermentation pH: natural；
Fermented type: anaerobic fermentation；
Moisture content in medium: 40%~60%；
Fermentation time: 48~72 hours.
7. the preparation method of the dedicated glycolysis fine fodder ensilage of a broad sow according to claim 1, which is characterized in that
The ensilage, fermentation fine fodder wet basis, aerobic fermentation strain liquid and mountain flour weight ratio be 100:1~1000:1~
100:0.1~10；
The aerobic fermentation strain liquid is made of barms liquid, bacillus subtilis bacterium solution and bacillus licheniformis liquid, wherein
The viable bacteria spore quantity ratio of barms liquid, bacillus subtilis bacterium solution and bacillus licheniformis liquid be 100:1~1000:1~
500；
The fermentation condition of step 4. is as follows:
Fermentation temperature: 26~32 DEG C；
Starting fermentation pH: natural；
Fermented type: oxygen consumption fermentation；
Moisture content in medium: 20~80%；
Fermentation time: 3~360 hours；
The above percentage is mass percent.
8. the preparation method of the dedicated glycolysis fine fodder ensilage of a broad sow according to claim 7, which is characterized in that
The ensilage, fermentation fine fodder wet basis, aerobic fermentation strain liquid and mountain flour weight ratio be 100:30~100:6~
12:0.1~1；
The aerobic fermentation strain liquid is made of barms liquid, bacillus subtilis bacterium solution and bacillus licheniformis liquid, wherein
Barms, bacillus subtilis and the viable bacteria of bacillus licheniformis spore quantity ratio are 100:1~1000:1~500；
The fermentation condition of step 4. is as follows:
Fermentation temperature: 26~32 DEG C；
Starting fermentation pH: natural；
Fermented type: oxygen consumption fermentation；
Moisture content in medium: 50%~60%；
Fermentation time: 24~48 hours.
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Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100405918C (en) * | 2005-04-29 | 2008-07-30 | 四川省畜牧科学研究院 | Ensiling method of sweet potato or sweet potato root tuber |
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-
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