CN112335783A - Mixed silage of sweet sorghum and lemon leaves and preparation method thereof - Google Patents
Mixed silage of sweet sorghum and lemon leaves and preparation method thereof Download PDFInfo
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- CN112335783A CN112335783A CN202011129767.6A CN202011129767A CN112335783A CN 112335783 A CN112335783 A CN 112335783A CN 202011129767 A CN202011129767 A CN 202011129767A CN 112335783 A CN112335783 A CN 112335783A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
- A23K30/10—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder
- A23K30/15—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging
- A23K30/18—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs of green fodder using chemicals or microorganisms for ensilaging using microorganisms or enzymes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a mixed silage of sweet sorghum and lemon leaves and a preparation method thereof, wherein the mixed silage is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; the ensiling raw materials comprise sweet sorghum straws and lemon leaves in a mass ratio of 75: 25-95: 5; the compound bacterial liquid comprises lactobacillus fermentation liquid, yeast fermentation liquid, bacillus subtilis fermentation liquid and enterococcus faecalis fermentation liquid; the cellulose compound enzyme comprises cellulase and hemicellulase in a mass ratio of 1: 1-4: 1. During preparation, the cellulose complex enzyme and the silage raw materials are mixed uniformly, then the complex bacteria liquid is sprayed on the surface of the mixture, and finally the mixture is fermented at room temperature in the dark for 55-65 days to obtain the silage. By adopting the technical scheme of the invention, the problem of low resource utilization rate can be solved while the nutritive value of the feed is improved, and a new way is opened up for feed supply.
Description
Technical Field
The invention belongs to the technical field of feed preparation, and particularly relates to a sweet sorghum and lemon leaf mixed silage and a preparation method thereof.
Background
Sweet sorghum stalks are rich in sugar, and besides being used as sugar and energy crop substances, sweet sorghum can also be used as pasture, hay, silage and silage, wherein silage is the most common and most efficient utilization form. The sugar content of the sweet sorghum is high, sufficient energy substrates can be provided for the lactic acid bacteria to grow, and the sweet sorghum has the characteristics of high content of soluble carbohydrate, low buffer energy value, quick reduction of pH value and the like, and is favorable for the preparation of silage. However, the sweet sorghum contains more tannin, has the effect of anti-nutritional factors, can inhibit rumen microorganisms, influence rumen fermentation and feed degradation, and can obviously reduce the feeding value of plants if the tannin content exceeds 2 percent of DM content; if it exceeds 5%, palatability and digestibility are affected, thereby lowering animal productivity. The sweet sorghum has long vegetative growth period, few seeds and high water content, so that the nutrient components of the sweet sorghum are lost when the sweet sorghum is directly ensiled, and environmental pollution can be caused.
The lemon leaves have unique flavor and are rich in functional active substances such as natural citric acid and the like, but the lemon leaves fed singly have poor palatability, low nutrient concentration and unstable nutrient components, the nutrients are easy to lose by special treatment such as squeezing, and the drying wastes labor and time, so most of the rest lemon is thrown away as waste, and the resources are wasted.
At present, the scale expansion of the breeding industry in China has the prominent problems of forage grass supply, seasonal and regional forage grass shortage and the like, and in addition to fully utilizing the straws of grain crops, unconventional feed resources such as cash crop byproducts, forest tree leaves and the like are urgently needed to be developed and utilized. The existing research reports show that sweet sorghum is superior to silage raw materials of corn, but the sweet sorghum has high water content and is easy to rot when being independently silage; a large amount of lemon leaves produced by pruning and shaping every year have low water content, are rich in active substances such as citric acid and the like, and are not recycled. However, no study on mixed silage of sweet sorghum and lemon leaves is reported.
Disclosure of Invention
Aiming at the prior art, the invention provides a sweet sorghum and lemon leaf mixed silage and a preparation method thereof, and aims to solve the problems of low resource utilization rate of lemon leaves and feed and forage supply shortage.
In order to achieve the purpose, the invention adopts the technical scheme that: providing a mixed silage of sweet sorghum and lemon leaves, wherein the mixed silage is prepared from a silage raw material, a compound bacterial liquid and a cellulose compound enzyme; the ensiling raw materials comprise sweet sorghum straws and lemon leaves in a mass ratio of 75: 25-95: 5; the compound bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis saturated bacillus fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, the saccharomycete, the bacillus subtilis saturated bacillus and the enterococcus faecalis in the compound bacterial liquid is 70-75: 45-55: 8-12: 5-10; the cellulose compound enzyme comprises cellulase and hemicellulase in a mass ratio of 1: 1-4: 1.
On the basis of the technical scheme, the invention can be further improved as follows.
Further, the mass ratio of sorghum straws to lemon leaves in the ensiling raw materials is 85: 15.
Furthermore, the ratio of the number of units formed by lactic acid bacteria, yeast, saturated bacillus subtilis and enterococcus faecalis in the mixed bacterial liquid is 72:50:10: 8.
Further, the mass ratio of the cellulase to the hemicellulase in the cellulose complex enzyme is 2: 1.
The mixed silage of sweet sorghum and lemon leaves is prepared by the following steps:
s1: pulverizing sweet sorghum straws and lemon leaves into small sections with the length of less than 2cm, and mixing according to a ratio to obtain silage raw materials;
s2: adding the cellulose complex enzyme into the silage raw materials according to the proportion of 5-8 mg/kg, and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 5-10 mL/kg;
s4: and (3) putting the mixture processed by the S3 into a vacuum packaging bag, vacuumizing, and fermenting for 55-65 days at 20-25 ℃ in a dark place to obtain the black fungus black tea.
The preparation method of the invention can be further improved based on the scheme.
Furthermore, the addition amount of the cellulose complex enzyme in the S2 is 6 mg/kg.
Further, the spraying amount of the compound bacterial liquid in S3 is 8 mL/kg.
Further, the fermentation temperature in S4 was 22 ℃ and the fermentation time was 60 days.
The invention has the beneficial effects that:
1. the invention takes the mixture of the sweet sorghum and the citric acid as the silage raw material, thereby not only avoiding resource waste and environmental pollution, but also opening up a new way for the source of the feed, improving the supply amount of the feed and effectively solving the problem of feed shortage.
2. According to the invention, the lemon leaves are added into the silage raw materials, the lemon leaves are rich in limonene and citric acid, and are fermented together with the sweet sorghum, so that the pH value of the feed can be reduced, the enzyme activity is improved, and the decomposition and absorption of proteins are promoted; the final feed has the effects of improving gastrointestinal microorganisms, inhibiting harmful microorganisms and promoting beneficial microorganisms; not only can stimulate the appetite of ruminants, but also can improve the feed intake and the digestibility.
3. The feed formula comprises a compound bacterial liquid and a cellulose compound enzyme. The composite bacterial liquid contains various beneficial bacterial groups, can accelerate the decomposition of the sweet sorghum straws and the lemon leaves, and forms a competitive relationship with other bacteria such as mould, saprophytic bacteria and the like, thereby avoiding the propagation of the other bacteria and ensuring the smooth fermentation; the cellulose compound enzyme can decompose the fibers of the sweet sorghum straws and the lemon leaves, so that the fibers are converted into nutrient substances such as polysaccharide, the final product has higher nutritional value, the fiber content is reduced, the palatability of the final product is enhanced, and the feed intake is higher.
Detailed Description
The following examples are provided to illustrate specific embodiments of the present invention.
Example 1
A mixed silage of sweet sorghum and lemon leaves is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; the ensiling raw materials comprise sweet sorghum straws and lemon leaves with the mass ratio of 85: 15; the compound bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis saturated bacillus fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, the saccharomycete, the bacillus subtilis saturated bacillus and the enterococcus faecalis in the compound bacterial liquid is 72:50:10: 8; the cellulose compound enzyme comprises cellulase and hemicellulase in a mass ratio of 2: 1.
The mixed silage in this example is prepared by the following steps:
s1: smashing sweet sorghum straws and lemon leaves into small sections with the length of less than 2cm, and mixing the sorghum straws and the lemon leaves according to the mass ratio of 85:15 to obtain silage raw materials;
s2: adding the cellulose complex enzyme into the silage raw materials according to the proportion of 6mg/kg (6 mg of the cellulose complex enzyme is added into each kilogram of the silage raw materials) and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 8 mL/kg;
s4: and (3) putting the mixture processed by the S3 into a vacuum packaging bag, vacuumizing, and fermenting for 60 days at 22 ℃ in a dark place.
Example 2
A mixed silage of sweet sorghum and lemon leaves is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; the ensiling raw materials comprise sweet sorghum straws and lemon leaves in a mass ratio of 75: 25; the compound bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis saturated bacillus fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, the saccharomycete, the bacillus subtilis saturated bacillus and the enterococcus faecalis in the compound bacterial liquid is 70:45:12: 5; the cellulose compound enzyme comprises cellulase and hemicellulase in a mass ratio of 1: 1.
The mixed silage in this example is prepared by the following steps:
s1: smashing sweet sorghum straws and lemon leaves into small sections with the length of less than 2cm, and mixing the sorghum straws and the lemon leaves according to the mass ratio of 75:25 to obtain silage raw materials;
s2: adding the cellulose complex enzyme into the silage raw materials according to the proportion of 8mg/kg (8 mg of the cellulose complex enzyme is added into each kilogram of the silage raw materials) and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 5 mL/kg;
s4: and (3) putting the mixture processed by the S3 into a vacuum packaging bag, vacuumizing, and fermenting for 55 days at 25 ℃ in a dark place to obtain the product.
Example 3
A mixed silage of sweet sorghum and lemon leaves is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; the ensiling raw materials comprise sweet sorghum straws and lemon leaves with the mass ratio of 95: 5; the compound bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis saturated bacillus fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, the saccharomycete, the bacillus subtilis saturated bacillus and the enterococcus faecalis in the compound bacterial liquid is 75:55:8: 10; the cellulose compound enzyme comprises cellulase and hemicellulase in a mass ratio of 4: 1.
The mixed silage in this example is prepared by the following steps:
s1: smashing sweet sorghum straws and lemon leaves into small sections with the length of less than 2cm, and mixing the sorghum straws and the lemon leaves according to the mass ratio of 95:5 to obtain silage raw materials;
s2: adding the cellulose complex enzyme into the silage raw materials according to the proportion of 5mg/kg (5 mg of the cellulose complex enzyme is added into each kilogram of the silage raw materials) and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 10 mL/kg;
s4: and (3) putting the mixture processed by the S3 into a vacuum packaging bag, vacuumizing, and fermenting for 65 days at 20 ℃ in a dark place.
Comparative example 1
A mixed silage feed of sweet sorghum and lemon leaves is prepared from silage raw materials and compound bacterial liquid; the ensiling raw materials comprise sweet sorghum straws and lemon leaves with the mass ratio of 85: 15; the compound bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis saturated bacillus fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, the saccharomycete, the bacillus subtilis saturated bacillus and the enterococcus faecalis in the compound bacterial liquid is 72:50:10: 8.
The mixed silage in this example is prepared by the following steps:
s1: smashing sweet sorghum straws and lemon leaves into small sections with the length of less than 2cm, and mixing the sorghum straws and the lemon leaves according to the mass ratio of 85:15 to obtain silage raw materials;
s2: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 8 mL/kg;
s3: and (3) putting the mixture processed by the S2 into a vacuum packaging bag, vacuumizing, and fermenting for 60 days at 22 ℃ in a dark place.
Comparative example 2
A mixed silage of sweet sorghum and lemon leaves is prepared from silage raw materials and cellulose complex enzyme; the ensiling raw materials comprise sweet sorghum straws and lemon leaves with the mass ratio of 85: 15; the cellulose compound enzyme comprises cellulase and hemicellulase in a mass ratio of 2: 1.
The mixed silage in this example is prepared by the following steps:
s1: smashing sweet sorghum straws and lemon leaves into small sections with the length of less than 2cm, and mixing the sorghum straws and the lemon leaves according to the mass ratio of 85:15 to obtain silage raw materials;
s2: adding the cellulose complex enzyme into the silage raw materials according to the proportion of 6mg/kg (6 mg of the cellulose complex enzyme is added into each kilogram of the silage raw materials) and uniformly mixing to obtain a mixture;
s3: and (3) putting the mixture processed by the S2 into a vacuum packaging bag, vacuumizing, and fermenting for 60 days at 22 ℃ in a dark place.
Comparative example 3
A silage is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; the ensiling raw material is sweet sorghum straw; the compound bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis saturated bacillus fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, the saccharomycete, the bacillus subtilis saturated bacillus and the enterococcus faecalis in the compound bacterial liquid is 72:50:10: 8; the cellulose compound enzyme comprises cellulase and hemicellulase in a mass ratio of 2: 1.
The mixed silage in this example is prepared by the following steps:
s1: smashing sweet sorghum straws into small sections with the length of less than 2cm to obtain ensiling raw materials;
s2: adding the cellulose complex enzyme into the silage raw materials according to the proportion of 6mg/kg (6 mg of the cellulose complex enzyme is added into each kilogram of the silage raw materials) and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 8 mL/kg;
s4: and (3) putting the mixture processed by the S3 into a vacuum packaging bag, vacuumizing, and fermenting for 60 days at 22 ℃ in a dark place.
Comparative example 4
A silage is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; the ensiling raw material is lemon leaves; the compound bacterial liquid comprises lactobacillus fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis saturated bacillus fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactobacillus, the saccharomycete, the bacillus subtilis saturated bacillus and the enterococcus faecalis in the compound bacterial liquid is 72:50:10: 8; the cellulose compound enzyme comprises cellulase and hemicellulase in a mass ratio of 2: 1.
The mixed silage in this example is prepared by the following steps:
s1: crushing lemon leaves into small sections with the length less than 2cm to obtain ensiling raw materials;
s2: adding the cellulose complex enzyme into the silage raw materials according to the proportion of 6mg/kg (6 mg of the cellulose complex enzyme is added into each kilogram of the silage raw materials) and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 8 mL/kg;
s4: and (3) putting the mixture processed by the S3 into a vacuum packaging bag, vacuumizing, and fermenting for 60 days at 22 ℃ in a dark place.
Analysis of results
1. Sensory evaluation
Sensory evaluation was carried out according to German Agri-culture Association (DLG) sensory rating standards for silage, as shown in Table 1. And directly observing and recording the color and the mildew condition of the mixed storage in each proportion after opening the bag, and evaluating the smell and the structure of the silage through smell sense and touch sense.
TABLE 1 sensory evaluation criteria
2. Analysis of nutrient composition
The contents of nutrients and mineral elements in dry matter DM, crude protein CP, neutral detergent fiber NDF, acid detergent fiber ADF, soluble carbohydrates WSC, crude fat EE, crude Ash, calcium Ca, phosphorus P, etc. in the mixed silage were analytically determined, and the results are shown in table 2.
TABLE 2 nutritional ingredients (dry matter) of the mixed silage of sorgo and lemon leaves
As can be seen from the table, by adopting the formula and the method of the invention, the mixed silage with high nutritional value and good palatability can be obtained.
Compared with the example 1, the raw materials are not added with the cellulose complex enzyme, the decomposition of the fibers in the sweet sorghum and the lemon leaves is limited in the fermentation process, and more crude fibers exist in the final product, so that the nutrition value of the product is not high, the palatability is poor, and the product is not accepted by livestock.
Compared with the example 1, the comparative example 2 has the advantages that the compound bacterial liquid is not added in the raw materials, the decomposition of microorganisms is lacked in the fermentation process, the fermentation effect is poor, the nutrient substances in the sweet sorghum straws and the lemon leaves cannot be released, and the content of the nutrient substances in the final product is low.
Compared with the example 1, the silage raw material only contains sweet sorghum which contains more tannin, has the effect of anti-nutritional factors, can inhibit the effect of microorganisms, influences fermentation and degradation, and causes low content of nutrient substances and poor palatability of a final product; moreover, the sweet sorghum has high water content, and is easy to mildew after being independently ensiled, thereby seriously affecting the quality of the feed.
Comparative example 4 compared with example 1, the ensiling raw material only has lemon leaves, the nutrient concentration is low, the nutrient content is unstable, the loss of nutrient substances is easily caused by single fermentation, and the palatability is poor and is not favored by livestock due to the influence of the characteristics of the lemon leaves by only adopting the lemon leaves for fermentation.
While the present invention has been described in detail with reference to the embodiments, it should not be construed as limited to the scope of the patent. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.
Claims (8)
1. A sweet sorghum and lemon leaf mixed silage is characterized in that: the mixed silage is prepared from silage raw materials, compound bacterial liquid and cellulose compound enzyme; the ensiling raw materials comprise sweet sorghum straws and lemon leaves in a mass ratio of 75: 25-95: 5; the compound bacterial liquid comprises lactic acid bacteria fermentation liquid, saccharomycete fermentation liquid, bacillus subtilis saturated bacillus fermentation liquid and enterococcus faecalis fermentation liquid, and the unit number ratio of the lactic acid bacteria, the saccharomycete, the bacillus subtilis saturated bacillus and the enterococcus faecalis in the compound bacterial liquid is 70-75: 45-55: 8-12: 5-10; the cellulose compound enzyme comprises cellulase and hemicellulase in a mass ratio of 1: 1-4: 1.
2. The mixed sorghum saccharatum and lemon leaf silage as claimed in claim 1, characterized in that: the mass ratio of sorghum straws to lemon leaves in the ensiling raw materials is 85: 15.
3. The mixed sorghum saccharatum and lemon leaf silage as claimed in claim 1, characterized in that: the ratio of the number of units formed by lactobacillus, saccharomycetes, bacillus subtilis and enterococcus faecalis in the mixed bacterial liquid is 72:50:10: 8.
4. The mixed sorghum saccharatum and lemon leaf silage as claimed in claim 1, characterized in that: the mass ratio of the cellulase to the hemicellulase in the cellulose complex enzyme is 2: 1.
5. The method for preparing the sweet sorghum and lemon leaf mixed silage according to any one of claims 1 to 4, comprising the following steps:
s1: pulverizing sweet sorghum straws and lemon leaves into small sections with the length of less than 2cm, and mixing according to a ratio to obtain silage raw materials;
s2: adding the cellulose complex enzyme into the silage raw materials according to the proportion of 5-8 mg/kg, and uniformly mixing to obtain a mixture;
s3: spraying the composite bacterial liquid on the surface of the mixture, wherein the spraying amount of the composite bacterial liquid is 5-10 mL/kg;
s4: and (3) putting the mixture processed by the S3 into a vacuum packaging bag, vacuumizing, and fermenting for 55-65 days at 20-25 ℃ in a dark place to obtain the black fungus black tea.
6. The method of claim 5, wherein: the addition amount of the cellulose complex enzyme in the S2 is 6 mg/kg.
7. The method of claim 5, wherein: the spraying amount of the compound bacterial liquid in the S3 is 8 mL/kg.
8. The method of claim 5, wherein: the fermentation temperature in S4 is 22 deg.C, and the fermentation time is 60 days.
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| CN115039835A (en) * | 2022-06-24 | 2022-09-13 | 山东农业大学 | Mixed silage of sweet sorghum and sesbania as well as preparation method and application of mixed silage |
| CN115462447A (en) * | 2022-10-06 | 2022-12-13 | 中国科学院亚热带农业生态研究所 | Method for preparing sorghum straw whole-plant corn mixed storage forage grass |
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