CN110973355A - Whole-plant hybrid broussonetia papyrifera biological feed leavening agent - Google Patents
Whole-plant hybrid broussonetia papyrifera biological feed leavening agent Download PDFInfo
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- CN110973355A CN110973355A CN201911233318.3A CN201911233318A CN110973355A CN 110973355 A CN110973355 A CN 110973355A CN 201911233318 A CN201911233318 A CN 201911233318A CN 110973355 A CN110973355 A CN 110973355A
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- broussonetia papyrifera
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- 241000705930 Broussonetia papyrifera Species 0.000 title claims abstract description 40
- 235000010855 food raising agent Nutrition 0.000 title claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 48
- 230000004151 fermentation Effects 0.000 claims abstract description 48
- 241000894006 Bacteria Species 0.000 claims abstract description 23
- 102000004190 Enzymes Human genes 0.000 claims abstract description 22
- 108090000790 Enzymes Proteins 0.000 claims abstract description 22
- 150000001413 amino acids Chemical class 0.000 claims abstract description 19
- 239000002131 composite material Substances 0.000 claims abstract description 16
- 230000009286 beneficial effect Effects 0.000 claims abstract description 15
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 15
- 239000005017 polysaccharide Substances 0.000 claims abstract description 15
- 241000933832 Broussonetia Species 0.000 claims description 28
- 239000011573 trace mineral Substances 0.000 claims description 27
- 235000013619 trace mineral Nutrition 0.000 claims description 27
- 235000001014 amino acid Nutrition 0.000 claims description 22
- 229940088598 enzyme Drugs 0.000 claims description 20
- 239000007858 starting material Substances 0.000 claims description 15
- 150000004804 polysaccharides Chemical class 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 12
- 238000002156 mixing Methods 0.000 claims description 12
- 229940088594 vitamin Drugs 0.000 claims description 12
- 229930003231 vitamin Natural products 0.000 claims description 12
- 235000013343 vitamin Nutrition 0.000 claims description 12
- 239000011782 vitamin Substances 0.000 claims description 12
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 9
- 235000001465 calcium Nutrition 0.000 claims description 9
- 239000011575 calcium Substances 0.000 claims description 9
- 229910052791 calcium Inorganic materials 0.000 claims description 9
- 150000001875 compounds Chemical class 0.000 claims description 9
- 241000411851 herbal medicine Species 0.000 claims description 9
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 8
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 8
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 8
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 8
- 240000008042 Zea mays Species 0.000 claims description 8
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 8
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 claims description 8
- 235000005822 corn Nutrition 0.000 claims description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
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- 239000004460 silage Substances 0.000 claims description 8
- 235000002639 sodium chloride Nutrition 0.000 claims description 8
- 239000004575 stone Substances 0.000 claims description 8
- 235000000346 sugar Nutrition 0.000 claims description 8
- 108010011619 6-Phytase Proteins 0.000 claims description 5
- 239000004382 Amylase Substances 0.000 claims description 5
- 108010065511 Amylases Proteins 0.000 claims description 5
- 102000013142 Amylases Human genes 0.000 claims description 5
- 102100032487 Beta-mannosidase Human genes 0.000 claims description 5
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 claims description 5
- 108091005804 Peptidases Proteins 0.000 claims description 5
- 108010059820 Polygalacturonase Proteins 0.000 claims description 5
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- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 5
- 235000019418 amylase Nutrition 0.000 claims description 5
- 108010055059 beta-Mannosidase Proteins 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 108010093305 exopolygalacturonase Proteins 0.000 claims description 5
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
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- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 claims description 4
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 4
- 241000194108 Bacillus licheniformis Species 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 4
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 4
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 claims description 4
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 4
- 241000222336 Ganoderma Species 0.000 claims description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 4
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- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 4
- 244000197580 Poria cocos Species 0.000 claims description 4
- 235000008599 Poria cocos Nutrition 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 claims description 4
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 4
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 claims description 4
- 229930003451 Vitamin B1 Natural products 0.000 claims description 4
- 229930003779 Vitamin B12 Natural products 0.000 claims description 4
- 229930003471 Vitamin B2 Natural products 0.000 claims description 4
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- 229930003316 Vitamin D Natural products 0.000 claims description 4
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 claims description 4
- 229930003427 Vitamin E Natural products 0.000 claims description 4
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 4
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 4
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 claims description 4
- -1 compound amino acid Chemical class 0.000 claims description 4
- 229910052802 copper Inorganic materials 0.000 claims description 4
- 239000010949 copper Substances 0.000 claims description 4
- RNGFNLJMTFPHBS-UHFFFAOYSA-L dipotassium;selenite Chemical compound [K+].[K+].[O-][Se]([O-])=O RNGFNLJMTFPHBS-UHFFFAOYSA-L 0.000 claims description 4
- 239000003651 drinking water Substances 0.000 claims description 4
- 235000020188 drinking water Nutrition 0.000 claims description 4
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 4
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 4
- 229910052740 iodine Inorganic materials 0.000 claims description 4
- 239000011630 iodine Substances 0.000 claims description 4
- 229910052742 iron Inorganic materials 0.000 claims description 4
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N iron oxide Inorganic materials [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 claims description 4
- 235000014655 lactic acid Nutrition 0.000 claims description 4
- 239000004310 lactic acid Substances 0.000 claims description 4
- 239000011777 magnesium Substances 0.000 claims description 4
- 229910052749 magnesium Inorganic materials 0.000 claims description 4
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 claims description 4
- 235000010755 mineral Nutrition 0.000 claims description 4
- 239000011707 mineral Substances 0.000 claims description 4
- NDLPOXTZKUMGOV-UHFFFAOYSA-N oxo(oxoferriooxy)iron hydrate Chemical compound O.O=[Fe]O[Fe]=O NDLPOXTZKUMGOV-UHFFFAOYSA-N 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 239000011574 phosphorus Substances 0.000 claims description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- 229960002477 riboflavin Drugs 0.000 claims description 4
- 239000011669 selenium Substances 0.000 claims description 4
- 229910052711 selenium Inorganic materials 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- 229910052708 sodium Inorganic materials 0.000 claims description 4
- 229960003495 thiamine Drugs 0.000 claims description 4
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 4
- 235000019155 vitamin A Nutrition 0.000 claims description 4
- 239000011719 vitamin A Substances 0.000 claims description 4
- 235000010374 vitamin B1 Nutrition 0.000 claims description 4
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- 239000011701 zinc Substances 0.000 claims description 4
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- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- FUFJGUQYACFECW-UHFFFAOYSA-L calcium hydrogenphosphate Chemical compound [Ca+2].OP([O-])([O-])=O FUFJGUQYACFECW-UHFFFAOYSA-L 0.000 claims description 3
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- 235000001715 Lentinula edodes Nutrition 0.000 claims description 2
- 239000004459 forage Substances 0.000 claims 1
- 238000000034 method Methods 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 22
- 239000001913 cellulose Substances 0.000 abstract description 9
- 229920002678 cellulose Polymers 0.000 abstract description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 7
- 238000006731 degradation reaction Methods 0.000 abstract description 7
- 239000008103 glucose Substances 0.000 abstract description 7
- 102000004169 proteins and genes Human genes 0.000 abstract description 7
- 108090000623 proteins and genes Proteins 0.000 abstract description 7
- 229920005610 lignin Polymers 0.000 abstract description 6
- 230000036983 biotransformation Effects 0.000 abstract description 5
- 230000005764 inhibitory process Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 4
- 150000007524 organic acids Chemical class 0.000 abstract description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract description 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 3
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- 150000002772 monosaccharides Chemical class 0.000 abstract description 2
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- 150000004676 glycans Chemical class 0.000 abstract 2
- 238000005516 engineering process Methods 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 6
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- 238000009395 breeding Methods 0.000 description 5
- 230000001488 breeding effect Effects 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 4
- 235000013339 cereals Nutrition 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 3
- 241000235342 Saccharomycetes Species 0.000 description 2
- 235000019764 Soybean Meal Nutrition 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 239000004455 soybean meal Substances 0.000 description 2
- 235000015099 wheat brans Nutrition 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000218231 Moraceae Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 210000004913 chyme Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000007918 intramuscular administration Methods 0.000 description 1
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- 238000004519 manufacturing process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
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- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Sustainable Development (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention discloses a whole hybrid broussonetia papyrifera biological feed leavening agent which comprises the following components in percentage by mass: 30-50% of complex enzyme, 30-45% of beneficial bacteria and 10-20% of fermentation accelerator; the product inhibition effect in the fermentation degradation process of the hybrid broussonetia papyrifera is obviously reduced by the organic synergistic effect of the complex enzyme, the composite beneficial bacteria and the fermentation accelerator in the fermentation process, so that the cellulose is degraded into glucose, organic acid and micromolecular polysaccharide, the lignin is degraded into micromolecular aromatic compounds, the broussonetia papyrifera protein is degraded into micromolecular short peptide or amino acid, the total biotransformation utilization rate reaches more than 80%, and the micromolecular polysaccharide can be further converted into monosaccharide to be absorbed by animals through endogenous enzyme of ingesting animals.
Description
Technical Field
The invention relates to the technical field of feed preparation, in particular to a leavening agent of a whole hybrid paper mulberry biological feed.
Background
The grain occupation of China is only 400kg per capita, and has a great difference with the grain occupation of 800kg per capita in developed countries, meanwhile, China is the first breeding country in the world, the breeding amount of various breeding varieties is more than 40% of the world, the food gap for feed is very large, especially the protein resource for feed is very short, and the annual import amount of soybean can be as high as 9000 thousands of tons. The development of wide biological resources to solve the contradiction of the shortage of protein resources for feed in China has become an important mission of the majority of agricultural science and technology workers.
The hybrid paper mulberry is a woody Moraceae plant which has high photosynthesis efficiency, less diseases, fast growth and rich nutrition, particularly has high protein content, and is greatly concerned by agricultural science and technology workers, but the whole hybrid paper mulberry has high lignin and cellulose content, large protein molecular weight and poor biotransformation utilization rate for direct feeding, the growth speed and feed reward of animals can be influenced when the use ratio of the total hybrid paper mulberry in the animal daily feed exceeds 5 percent, and the improvement of the biotransformation utilization rate is very limited even through ensiling or shallow fermentation, so that the application of the total hybrid paper mulberry in the animal feeding daily feed is greatly limited.
Most monogastric animals cannot effectively utilize plant stems and leaves because the main components of the plant stems and leaves are cellulose and lignin, cellulose (C6H10O5) n is a macromolecular polymer of glucose (C6H12O6) and can be degraded into the glucose through enzymatic hydrolysis under certain conditions, the glucose is the most important energy nutrient substance of the animals, and the problems of high cost, long period, poor product flavor and the like exist when the plant stems and leaves are degraded by purely depending on a complex enzyme preparation, most of the technologies use compound microorganisms for fermentation and degradation, but feedback inhibition can be quickly generated in the fermentation process, so that the cellulose and the lignin are not fully degraded, the effective products of the glucose, the organic acid, the small molecular polysaccharide and the like are few, and when the fermentation products are used in the ration of the monogastric animals in large quantity, the excessive undegraded cellulose cannot be the nutrient substance, on the contrary, the evacuation speed of intestinal chyme of animals can be accelerated, the absorption of the animals on nutrient substances is influenced, the normal growth of the animals is further hindered, the feeding period is prolonged, the feed efficiency is reduced, the feed cost is improved, most macromolecular proteins contained in the broussonetia papyrifera are not fully degraded, the biotransformation efficiency is low, although in recent years, a plurality of applied bacterium-enzyme composite fermentation technologies improve the degradation efficiency, the application of the broussonetia papyrifera biological fermentation feed in the feeding of monogastric animals is realized, the feed is also limited by the feedback inhibition effect, the degradation of cellulose and lignin is still insufficient, the daily ration consumption of the single-gastric animals of the plurality of broussonetia papyrifera biological fermentation feeds cannot exceed 8%, the contribution to the reduction of the feed cost is limited, and the popularization and the development of the broussonetia pap.
Disclosure of Invention
Therefore, the invention provides a starter of a whole-plant hybrid broussonetia papyrifera biological feed, which aims to overcome the defects of slow fermentation speed, insufficient degradation, short quality guarantee period of the fermented feed, low daily ration use ratio and high feed cost in the existing hybrid broussonetia papyrifera feed application technology.
The invention is realized by the following technical means:
a whole-plant hybrid broussonetia papyrifera biological feed leavening agent comprises the following components in percentage by mass: 30-50% of complex enzyme, 30-45% of beneficial bacteria and 10-20% of fermentation accelerator.
Preferably, the invention discloses a whole hybrid broussonetia papyrifera biological feed leavening agent which comprises the following components in percentage by mass: 43% of compound enzyme, 40% of beneficial bacteria and 17% of fermentation accelerator.
Preferably, the compound enzyme consists of the following components in percentage by mass: 1-15% of cellulase, 1-8% of mannase, 1-12% of xylanase, 3-15% of amylase, 1-7% of pectinase, 3-12% of protease, 1-15% of ligninase and 3-10% of phytase.
Preferably, the beneficial bacteria comprise the following components in percentage by mass: 1-15% of yeast, 1-25% of bacillus subtilis, 1-20% of bacillus licheniformis, 1-20% of photosynthetic bacteria and 1-40% of lactic acid bacteria.
Preferably, the fermentation promoter consists of the following components in percentage by mass: 10-30% of amino acid chelated trace elements, 10-35% of xylan, 5-20% of Chinese herbal medicine polysaccharide and 5-20% of composite trace elements.
Preferably, the amino acid chelated trace element is chelated by the following components in parts by weight: 300-600 parts of compound amino acid aqueous solution, 30-60 parts of potassium selenite, 20-40 parts of ferric oxide and 10-60 parts of zinc oxide, wherein the Chinese herbal medicine polysaccharide is prepared by decocting the following components in parts by weight: 10-30 parts of lucid ganoderma, 30-50 parts of poria cocos and 50-80 parts of lentinus edodes, wherein the composite trace elements consist of the following elements in parts by weight: 10-30 parts of potassium, 10-40 parts of magnesium, 20-50 parts of sodium, 10-70 parts of calcium, 10-50 parts of calcium carbonate, 20-60 parts of phosphorus and 10-100 parts of mineral water.
Preferably, the invention also discloses a complete pasture biological fermentation feed prepared by adopting the complete plant hybrid broussonetia papyrifera biological feed leavening agent, which comprises the following components in percentage by mass: 0.05-0.15% of full-plant hybrid broussonetia papyrifera biological feed leavening agent, 10-80% of main material, 1-4% of auxiliary material and 1-80% of drinking water.
Preferably, the main material is prepared by mixing fresh branches or silage, corn and cake meal of a whole hybrid paper mulberry, and the auxiliary material is prepared by mixing edible sugar, salt, calcium hydrophosphate, feeding stone powder, amino acid, trace elements and vitamins.
Preferably, the microelements comprise a plurality of iron, manganese, zinc, copper, iodine and selenium, and the vitamins comprise a plurality of vitamin A, vitamin B1, vitamin B2, vitamin B12, vitamin C, vitamin D and vitamin E.
Preferably, the preparation method of the complete hybrid broussonetia papyrifera biological fermentation feed comprises the following steps: mixing whole hybrid paper mulberry biological feed starter, whole hybrid paper mulberry fresh branches or silage, corn, cake meal, sugar, salt, calcium hydrogen phosphate, feeding stone powder, amino acid, trace elements and vitamins uniformly, and fermenting.
Preferably, in the preparation process of the complete grass biological fermentation feed, before mixed fermentation, fresh branches or silage of the whole hybrid paper mulberry are crushed into particles with the diameter not larger than 3mm, after crushing, all the raw materials are placed in a sealed container or other sealed packages and fermented for more than 72 hours at the temperature of 25-35 ℃, and the feed can be used for feeding when the pH value is 4-5.
The invention has the beneficial effects that:
on the basis of a bacterial enzyme composite fermentation technology, the invention finely researches the coordination mechanism of various enzymes and enzyme-producing microorganisms, reasonably prepares a plurality of fermentation promoters for promoting the enzyme activity and continuously and rapidly propagating the enzyme-producing microorganisms, better solves the feedback inhibition in the fermentation degradation process, more fully degrades cellulose into nutrient substances such as glucose, organic acid, micromolecule polysaccharide and the like which can be absorbed and utilized by monogastric animals, and degrades the broussonetia papyrifera macromolecular protein into micromolecule short peptide which can be directly absorbed and utilized;
the whole hybrid broussonetia papyrifera biological feed leavening agent consists of three components:
enzymes: cellulase, mannanase, xylanase, amylase, pectinase, protease, ligninase, phytase;
beneficial bacteria: yeast, bacillus, lactobacillus, and photosynthetic bacteria;
accelerators: amino acid chelated microelements, xylan, Chinese herbal medicine polysaccharide and composite microelements.
The product inhibition effect in the fermentation degradation process of the hybrid broussonetia papyrifera is obviously reduced by the organic synergistic effect of the complex enzyme, the composite beneficial bacteria and the fermentation accelerator in the fermentation process, so that the cellulose is degraded into glucose, organic acid and micromolecular polysaccharide, the lignin is degraded into micromolecular aromatic compounds, the broussonetia papyrifera protein is degraded into micromolecular short peptide or amino acid, the total biotransformation utilization rate reaches more than 80%, and the micromolecular polysaccharide can be further converted into monosaccharide to be absorbed by animals through endogenous enzyme of ingesting animals.
The specific implementation mode is as follows:
in order to make the technical solutions of the present invention better understood by those skilled in the art, the present invention is further described below with reference to the following examples:
example 1
The invention discloses a whole-plant hybrid broussonetia papyrifera biological feed leavening agent which comprises the following components in percentage by mass: 43% of compound enzyme, 40% of beneficial bacteria and 17% of fermentation accelerator.
The compound enzyme comprises the following components in percentage by mass: 15% of cellulase, 8% of mannase, 15% of xylanase, 10% of amylase, 15% of pectinase, 10% of protease, 15% of ligninase and 12% of phytase.
The beneficial bacteria comprise the following components in percentage by mass: 15% of saccharomycete, 25% of bacillus subtilis, 20% of bacillus licheniformis, 10% of photosynthetic bacteria and 30% of lactic acid bacteria;
the fermentation accelerant consists of the following components in percentage by mass: 30% of amino acid chelated trace elements, 30% of xylan, 20% of Chinese herbal medicine polysaccharide and 20% of composite trace elements.
The amino acid chelated trace element is chelated by the following components in parts by weight: 450 parts of compound amino acid aqueous solution, 45 parts of potassium selenite, 30 parts of ferric oxide and 35 parts of zinc oxide, wherein the Chinese herbal medicine polysaccharide is prepared by decocting the following components in parts by weight: 15 parts of lucid ganoderma, 40 parts of poria cocos and 60 parts of shiitake mushroom, wherein the composite trace elements consist of the following elements in parts by weight: 20 parts of potassium, 30 parts of magnesium, 30 parts of sodium, 40 parts of calcium, 25 parts of calcium carbonate, 40 parts of phosphorus and 60 parts of mineral water.
The invention also discloses a complete hybrid broussonetia papyrifera biological fermented feed prepared by adopting the complete hybrid broussonetia papyrifera biological feed leavening agent, which comprises the following components in percentage by mass: 0.08% of starter of biological feed for whole hybrid paper mulberry, 43% of fresh branches of whole hybrid paper mulberry, 20% of corn, 7% of soybean meal, 8% of wheat bran, 2% of auxiliary material and 20% of drinking water.
The auxiliary materials are formed by mixing edible sugar, salt, calcium hydrophosphate, feeding stone powder, amino acid, trace elements and vitamins.
The trace elements comprise a plurality of iron, manganese, zinc, copper, iodine and selenium, and the vitamins comprise a plurality of vitamin A, vitamin B1, vitamin B2, vitamin B12, vitamin C, vitamin D and vitamin E.
The preparation method of the complete hybrid paper mulberry biological fermentation feed comprises the following steps: according to the mass percentage, the starter culture of the whole hybrid broussonetia papyrifera biological feed, the fresh branches of the whole hybrid broussonetia papyrifera, the corn, the cake, the sugar, the salt, the calcium hydrophosphate, the feeding stone powder, the amino acid, the trace elements and the vitamins are mixed uniformly and fermented.
In the preparation process of the complete hybrid paper mulberry biological fermentation feed, before mixed fermentation, fresh branches of a whole hybrid paper mulberry are required to be crushed into particles with diameters not larger than 3mm, after crushing, blending and mixing are completed, all raw materials are placed in a sealed container and fermented for 72 hours at the temperature of 25-35 ℃, and the feed can be used for feeding when the pH value is 4.5.
Example 2
The invention discloses a whole-plant hybrid broussonetia papyrifera biological feed leavening agent which comprises the following components in percentage by mass: 43% of compound enzyme, 40% of beneficial bacteria and 17% of fermentation accelerator.
The compound enzyme comprises the following components in percentage by mass: 15% of cellulase, 8% of mannase, 15% of xylanase, 10% of amylase, 15% of pectinase, 10% of protease, 15% of ligninase and 12% of phytase.
The beneficial bacteria comprise the following components in percentage by mass: 15% of saccharomycete, 25% of bacillus subtilis, 20% of bacillus licheniformis, 10% of photosynthetic bacteria and 30% of lactic acid bacteria;
the fermentation accelerant consists of the following components in percentage by mass: 30% of amino acid chelated trace elements, 30% of xylan, 20% of Chinese herbal medicine polysaccharide and 20% of composite trace elements.
The amino acid chelated trace element is chelated by the following components in parts by weight: 450 parts of compound amino acid aqueous solution, 45 parts of potassium selenite, 30 parts of ferric oxide and 35 parts of zinc oxide, wherein the Chinese herbal medicine polysaccharide is prepared by decocting the following components in parts by weight: 15 parts of lucid ganoderma, 40 parts of poria cocos and 60 parts of shiitake mushroom, wherein the composite trace elements consist of the following elements in parts by weight: 20 parts of potassium, 30 parts of magnesium, 30 parts of sodium, 40 parts of calcium, 25 parts of calcium carbonate, 40 parts of phosphorus and 60 parts of mineral water.
The invention also discloses a complete hybrid broussonetia papyrifera biological fermented feed prepared by adopting the complete hybrid broussonetia papyrifera biological feed leavening agent, which comprises the following components in percentage by mass: 0.08 percent of leaven of the whole hybrid paper mulberry biological feed, 30 percent of the whole silage hybrid paper mulberry, 23 percent of corn, 8 percent of soybean meal, 12 percent of wheat bran, 2 percent of auxiliary material and 25 percent of drinking water.
The auxiliary materials are formed by mixing edible sugar, salt, calcium hydrophosphate, feeding stone powder, amino acid, trace elements and vitamins.
The trace elements comprise a plurality of iron, manganese, zinc, copper, iodine and selenium, and the vitamins comprise a plurality of vitamin A, vitamin B1, vitamin B2, vitamin B12, vitamin C, vitamin D and vitamin E.
The preparation method of the complete hybrid paper mulberry biological fermentation feed comprises the following steps: according to the mass percentage, the whole hybrid broussonetia papyrifera biological feed leaven, the whole silage hybrid broussonetia papyrifera, corn, cake meal, sugar, salt, calcium hydrogen phosphate, feeding stone powder, amino acid, trace elements and vitamins are mixed uniformly and fermented.
In the preparation process of the complete hybrid paper mulberry biological fermentation feed, before mixed fermentation, the whole plant of silage hybrid paper mulberry needs to be crushed into particles with the average diameter not larger than 3mm, after crushing, blending and mixing are completed, all raw materials are placed in a sealed container and fermented for 72 hours at the temperature of 25-35 ℃, and the feed can be used for feeding when the pH value is 4.5.
According to the product prepared in the embodiment 1-2, the complete broussonetia papyrifera biological fermentation feed produced by the technology can be used for feeding multiple animals by 10-100% of the daily ration of the animals, the cost of the feed is reduced by more than 10-30%, the feed grain is saved by more than 10-60%, the growth speed or the production performance of the cultured animals is obviously improved, the immunity and the health level of the cultured animals are obviously improved, the use of antibiotics is reduced and even completely replaced, and the detection of an authoritative detection mechanism proves that the cholesterol of the animals fed by the complete broussonetia papyrifera biological fermentation feed is reduced by more than 40%, the intramuscular fat is improved by more than 60%, and the content of unsaturated fatty acid reaches more than 60%.
By taking the technology of the invention as a core, the biological fermentation feed for the hybrid paper mulberry is produced by planting the hybrid paper mulberry in the breeding farm by using the excrement and the hybrid paper mulberry as the basic raw material and is used for feeding animals in the farm, all the excrement in the farm can be completely consumed by planting 400 mu of the hybrid paper mulberry in a slaughter ten-thousand pig farm every year, the grain for the feed can be saved by more than 700 tons each year, the cost of the feed is reduced by more than 100 ten thousand yuan, a completely closed ecological cycle chain is realized, and the pollution of the breeding industry area source, the water source and the atmospheric environment is fundamentally solved.
While certain exemplary embodiments of the invention have been described above by way of illustration only, it will be apparent to those skilled in the art that the described embodiments may be modified in various different ways without departing from the scope of the invention. Accordingly, the description is illustrative in nature and is not to be construed as limiting the scope of the invention as claimed.
Unless defined otherwise, all academic and scientific terms used herein have the same meaning as is understood by one of ordinary skill in the art to which this invention belongs.
In case of conflict, the present specification, including definitions, will control.
All percentages, parts, ratios, etc., are by weight unless otherwise indicated.
When a value or range of values, preferred range or list of lower preferable values and upper preferable values is given, it should be understood that it specifically discloses any range formed by any pair of values of any lower range limit or preferred value and any upper range limit or preferred value, regardless of whether ranges are separately disclosed. Where a range of numerical values is described herein, unless otherwise stated, the range is intended to include the endpoints of the range and all integers and fractions within the range.
When the term "about" or "approximately" is used to describe a numerical value or an end of a range, the disclosure should include the specific numerical value or end point referred to.
The use of "a" and "an" are merely for convenience and to give a general sense of the invention to describe elements and components of the invention. Unless expressly stated otherwise, this description should be read to include one or at least one.
Claims (10)
1. A whole-plant hybrid broussonetia papyrifera biological feed leavening agent is characterized in that: the composite material comprises the following components in percentage by mass: 30-50% of complex enzyme, 30-45% of beneficial bacteria and 10-20% of fermentation accelerator.
2. The starter of whole hybrid broussonetia papyrifera biological feed according to claim 1, wherein the starter is characterized in that: the composite material comprises the following components in percentage by mass: 43% of compound enzyme, 40% of beneficial bacteria and 17% of fermentation accelerator.
3. The starter of whole hybrid broussonetia papyrifera biological feed according to claim 1, wherein the starter is characterized in that: the compound enzyme comprises the following components in percentage by mass: 1-15% of cellulase, 1-8% of mannase, 1-12% of xylanase, 3-15% of amylase, 1-7% of pectinase, 3-12% of protease, 1-15% of ligninase and 3-10% of phytase.
4. The starter of whole hybrid broussonetia papyrifera biological feed according to claim 1, wherein the starter is characterized in that: the beneficial bacteria comprise the following components in percentage by mass: 1-15% of yeast, 1-25% of bacillus subtilis, 1-20% of bacillus licheniformis, 1-20% of photosynthetic bacteria and 1-40% of lactic acid bacteria.
5. The starter of whole hybrid broussonetia papyrifera biological feed according to claim 1, wherein the starter is characterized in that: the fermentation accelerant consists of the following components in percentage by mass: 10-30% of amino acid chelated trace elements, 10-35% of xylan, 5-20% of Chinese herbal medicine polysaccharide and 5-20% of composite trace elements.
6. The starter of claim 5 for biological feed of whole hybrid broussonetia papyrifera, wherein the starter is characterized in that: the amino acid chelated trace element is chelated by the following components in parts by weight: 300-600 parts of compound amino acid aqueous solution, 30-60 parts of potassium selenite, 20-40 parts of ferric oxide and 10-60 parts of zinc oxide, wherein the Chinese herbal medicine polysaccharide is prepared by decocting the following components in parts by weight: 10-30 parts of lucid ganoderma, 30-50 parts of poria cocos and 50-80 parts of lentinus edodes, wherein the composite trace elements consist of the following elements in parts by weight: 10-30 parts of potassium, 10-40 parts of magnesium, 20-50 parts of sodium, 10-70 parts of calcium, 10-50 parts of calcium carbonate, 20-60 parts of phosphorus and 10-100 parts of mineral water.
7. A complete forage biological fermentation feed is characterized in that: the composite material comprises the following components in percentage by mass: 0.05-0.15% of whole hybrid broussonetia papyrifera biological feed leaven, 10-80% of main material, 1-4% of auxiliary material and 1-80% of drinking water.
8. The complete pasture biological fermentation feed as claimed in claim 7, which is characterized in that: the main material is formed by mixing fresh branches or silage, corn and cake meal of a whole hybrid paper mulberry, and the auxiliary material is formed by mixing sugar, salt, calcium hydrophosphate, feeding stone powder, amino acid, trace elements and vitamins.
9. The complete pasture biological fermentation feed as claimed in claim 8, characterized in that: the trace elements comprise a plurality of iron, manganese, zinc, copper, iodine and selenium, and the vitamins comprise a plurality of vitamin A, vitamin B1, vitamin B2, vitamin B12, vitamin C, vitamin D and vitamin E.
10. The method for preparing a complete hybrid broussonetia papyrifera biological fermentation feed as claimed in claim 7, wherein the complete hybrid broussonetia papyrifera biological fermentation feed comprises the following steps: mixing whole hybrid paper mulberry biological feed starter, whole hybrid paper mulberry fresh branches or silage, corn, cake meal, sugar, salt, calcium hydrogen phosphate, feeding stone powder, amino acid, trace elements and vitamins uniformly and fermenting; before mixed fermentation, the fresh branches or ensiling of the whole hybrid paper mulberry are crushed into particles with the diameter not larger than 3mm on average, and after crushing, all the raw materials are placed in a sealed container or other sealed packages and fermented for more than 72 hours at the temperature of 25-35 ℃, and the mixed fermentation can be used for feeding when the pH value is between 4 and 5.
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CN111642640A (en) * | 2020-04-27 | 2020-09-11 | 皖西学院 | Broussonetia papyrifera feed vegetable protein starter culture for pigs and preparation method thereof |
CN112266883A (en) * | 2020-10-26 | 2021-01-26 | 中商构能生态科技(天津)有限公司 | Zymophyte liquid for whole-plant fermentation of hybrid paper mulberry, and preparation method and application thereof |
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CN111642640A (en) * | 2020-04-27 | 2020-09-11 | 皖西学院 | Broussonetia papyrifera feed vegetable protein starter culture for pigs and preparation method thereof |
CN112266883A (en) * | 2020-10-26 | 2021-01-26 | 中商构能生态科技(天津)有限公司 | Zymophyte liquid for whole-plant fermentation of hybrid paper mulberry, and preparation method and application thereof |
CN112515036A (en) * | 2020-11-30 | 2021-03-19 | 博益德(北京)生物科技有限公司 | Biological feed containing functional oligosaccharide and preparation method thereof |
CN112913979A (en) * | 2021-03-19 | 2021-06-08 | 泓森(嘉兴)生物技术有限公司 | Modified paper mulberry compound feed composition and preparation method and application thereof |
CN113812516A (en) * | 2021-09-26 | 2021-12-21 | 皖西学院 | Compound glycosyl biological feed leavening agent and preparation method and application thereof |
CN115843939A (en) * | 2022-10-31 | 2023-03-28 | 浙江大学 | Fermented feed for improving production performance of Taihe black-bone chicken and egg quality of Taihe black-bone chicken and application of fermented feed |
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