CN102119768A - Complex enzyme preparation for feeding piglets - Google Patents
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Abstract
The invention discloses a complex enzyme preparation for feeding piglets. The complex enzyme preparation for feeding piglets comprises the following seven enzymes: acid protease, amylase, xylanase, beta-glucanase, cellulase, pectinase and phytase; and the enzymatic activity ratio of the seven enzymes is 1: 1: (8-10): (3.3-4): 1: (0.5-0.55): (0-0.02). By applying the complex enzyme preparation disclosed by the invention, various anti-nutritional factors in feed can be degraded, the viscosity of chyme in an intestinal tract can be reduced, and the metabolic energy of the feed can be improved; the protein and starch digestibility of the piglets can be improved; excessive reproduction of harmful microorganisms in the intestinal tract can be reduced, damage to the intestinal wall can be reduced, and the microecological balance of the intestinal tract can be regulated; the survival rate and disease resistance of the piglets can be improved, the diarrhea rate can be reduced, and the overall uniformity is improved; and the weight gaining of the piglets can be promoted, and the cultivation cost is reduced.
Description
Technical field
The present invention relates to a kind of piglet complex enzyme preparation for feeding.
Background technology
Complex enzyme for feed is the novel activated feed additive of a class, is the mix preparation of multiple digestive ferment, and its major function factor is endogenous digestive ferment and exogenous digestive ferment.The endogenous digestive ferment mainly comprises amylase, protease and lipase etc.Exogenous digestive ferment is the similar enzyme that alimentary canal can not be secreted, and comprises cellulase, pectase, galactosidase, 1,4 beta-glucanase and phytase etc.Exogenous enzymes can not directly digest the big molecular nutrition material of hydrolysis, but the ANFs in hydrolysis or the hydrolysate feed, promotes the digestibility and utilization of nutriment indirectly.
Complex enzyme formulation mainly contains following a few class: (1) is mainly used in the deficiency of replenishing the animal endogenous enzymes based on the complex enzyme for feed of protease, amylase; (2), be mainly used in feedstuff based on barley, oat based on the complex enzyme for feed of 1,4 beta-glucanase; (3) based on the complex enzyme for feed of cellulase, pectase, main effect is to destroy plant cell wall, discharges the nutriment in the cell, eliminates the ANFs in the feed simultaneously, reduces the viscosity of gastrointestinal content, promotes digesting and assimilating of animal; (4) based on the complex enzyme for feed of cellulase, protease, amylase, carbohydrase, 1,4 beta-glucanase, pectase, the acting in conjunction of comprehensive various enzymes has stronger assist digestion effect.
The enzyme source of fodder enzyme preparation mainly comes from animal, plant, microorganism.The enzyme that derives from animal and plant generally is to use to contain the enzyme tissue extraction, and it is tissue-derived limited to contain enzyme, and price is higher, does medicinal, very difficult a large amount of as the feed enzyme sources more.Microorganism is the main source of production enzyme preparation.The product enzyme high-yield strains of producing complex enzyme for feed generally has: (1) fungi: aspergillus, wood are mould, saccharomycete etc.; (2) bacterium: bacillus, hay bacillus etc.; (3) engineering bacteria (engineering bacterium, engineering saccharomycete) etc.
Complex enzyme is the homogeneous mixture system of plurality of enzymes.The production of complex enzyme for feed is mainly by following 3 approach:
(1) single enzyme is composite, promptly adopts single strain liquid submerged fermentation manufacture order enzyme, and removing thalline, to carry out the extraction of single enzyme refining, mixes composite formation complex enzyme product by various single enzymes again.(2) produce the single culture fermentation of plurality of enzymes, normally adopt and to produce the cultivation of enzyme list bacterium and in containing multiple culture medium of inducing substrate, realize by solid fermentation.(3) producing the mixed culture fermentation of many bacterium of enzyme, also is to adopt solid fermentation to be about to two or more zymogenic bacteria kinds to cultivate in solid medium by certain technological design Mixed culture, realizes producing enzyme.
Complex enzyme improves the mechanism of feed nutritive value
(1) amylase: amylase mainly contains AMS and carbohydrase.AMS is a restriction endonuclease, starch polymer can be decomposed into easily absorbed middle lower-molecular substance; Carbohydrase also is a restriction endonuclease, and carbohydrase can further be hydrolyzed to glucose with the zymolytic middle lower-molecular substance of alphalise starch, is absorbed by animal.
(2) zytase: xylan is one of main composition composition of plant cell wall, animal and fowl fodder is many based on plant feed, the complex structure of plant cell wall mainly is made up of NSP (comprising xylan, cellulose, glucan, mannosan and pectin) and lignin.After even many feed processing are handled, still can not destroy the integrality of its cell membrane, be embedded in the interior many nutriments (protein, starch etc.) that digest of cell membrane owing to not contacting and can not be discharged from ight soil with digestive ferment by digestibility and utilization.Thereby zytase is broken the robust construction of cell membrane by degradation of xylan, the nutrient that is included in the cell is released, thereby improves the digestibility of this part nutrition; Reduce the viscosity of intestines and stomach chyme, for nonruminant, SNSPs such as the xylan that contains in the feed (NSP), can not be digested, can increase the stickiness of food, hinder the digestion of nutriment (fat and protein etc.), so xylan is called as a kind of " ANFs ".And the bonding pad that zytase can destroy covalent cross-linking in the xylan molecule and form by hydrogen bond, effectively degradation of xylan reduces the viscosity of food in the intestines, and performance promotes growth and the effect that improves feed efficiency; Reduce enteric microorganism quantity, reduce disease, be beneficial to health, the increase of enteron aisle viscosity causes nutriment to be accumulated in enteron aisle, forms the chyme that is rich in nutrient, and microorganism is here fermented, infringement intestinal mucosa normal morphology and function.There are some researches show, add xylanase preparation in wheat diet or the rye diet and can reduce bacterial population in the gastrointestinal tract of livestock and fowls, reduce the available nutriment of hindgut pathogen, reduced the incidence of disease of diarrhoea; Improve the activity of endogenous digestive ferment, in feed, add xylanase preparation, can improve the activity of endogenous digestive ferment, promote digesting and assimilating of nutrient.Because NSP can increase the viscosity of intestines and stomach chyme, the chance that full-bodied food has stoped digestive ferment and substrate to be in contact with one another in the small intestine has digested nutrient simultaneously and has slowed down to the intestinal mucosa diffusion velocity, thereby hindered digesting and assimilating of nutritional labeling; And NSP also can with the direct complexing of digestive ferment, thereby reduce the activity of various digestive ferments.Add zytase can be in animal intestinal degradation of xylan, the xylan of bar structure complexity is decomposed into monose, reduced the adverse effect of NSP to the enteron aisle chyme, eliminate the adverse effect of NSP to digestive ferment, improved the diffusion velocity of endogenous enzyme-to-substrate, promote that substrate combines with endogenous enzymes, help improving the stability of various digestive ferments with active.Thereby the raising endogenous enzyme activity makes the motionless water layer attenuation of mucous membrane surface simultaneously, promotes digesting and assimilating of nutrient.
(3) 1,4 beta-glucanase: corn is main energy feed always, but the supply day of corn is becoming tight.The abundant wheat class feed of application is made energy feed and is more and more come into one's own.Beta glucan is the principal element that restriction wheat class feed effectively utilizes, and many studies show that added the anti-oxidant action that 1,4 beta-glucanase can be eliminated beta glucan in wheat class daily ration both at home and abroad, improves the nutritive value of wheat class diet.The cell membrane that is made of SNSPs such as beta glucans is wrapped in nutrients such as starch, protein, stops the interaction of itself and digestive ferment.Because the endocrine 1,4 beta-glucanase deficiency of livestock and poultry body, this inhibition may be quite big; Beta glucan can be partly dissolved after entering alimentary canal, increases the viscosity of chyme, and the meeting imbibition increases the volume of enteron aisle.Any increase of the volume of intestinal contents and viscosity all can reduce the diffusivity of enzyme and substrate, stops their effecting reactions on intestinal mucosa.After the small intestine contents stickiness increases, can significantly increase the time of chyme in the enteron aisle stop, reduce the assimilation of nutrient in the unit interval, the nutrient that obstruction is digested is near the small intestinal mucosa surface, the digestive utilization ratio of feed fat, protein and carbohydrate is reduced, cause the production performance of livestock and poultry to reduce.Beta glucan combines with glycoprotein on the small intestine epithelium mucous membrane cell, form the motionless water layer of one deck thickening, limit its infiltration rate, beta glucan causes animal digestive system physiology and modal change simultaneously, cause rolling up of water, protein, electrolyte and the endogenous secretion of lipid, the consumption of this metabolic process will be quite big.The result of this variation is that digestive organs increase, and digestive juice secretion increases, but nutrient absorbing descends.When feeding high beta glucan barley the pancreas hypertrophy can take place, this is owing to pancreas is that compensatory pancreatin combines with beta glucan and increases the result of secretion; Beta glucan is not digested at the top enteron aisle in the diet, enters the carbon source that lower intestine becomes anaerobe fermentation propagation, thus produce anaerobes such as a large amount of clostridium sporogenes at the enteron aisle back segment, thus wherein some clostridium sporogenes produces toxin inhibition growth of animals or poultry.Intestinal bacterium quantity increases in addition can stimulate enteron aisle, thickens the intestinal mucosa layer, the infringement microvillus, thus reduce nutrient absorbing.Beta glucan and some digestive ferment combinations reduce its activity, and while and bile salt, lipid, cholesterol combination influence the small intestine lipid metabolism.The reduction of digestive enzyme activity such as trypsase, lipase with significantly increasing the secretion of endogenous substances such as protein, lipid and electrolyte, reduces their deposits in vivo.Generally speaking, add the digestibility that 1,4 beta-glucanase can improve various nutrients in the wheat class daily ration, and Multitest proves that 1,4 beta-glucanase can improve daily gain and feed efficiency.Utilize 1,4 beta-glucanase can yet be regarded as to improve the feeding value of wheat class raw material, improve one of effective way of existing efficiency of feed utilization.
(4) cellulase: cellulase comprises endoglucanase, glucan excision enzyme or cellobiase and beta-glucosidase.Cellulase can promote the dissolving of plant cell wall that more plant cell Dissolve things inside is dissolved out and can become small-molecule substance to help digesting and assimilating of animal gastrointestinal tract stodgy macromolecular polysaccharide, protein and lipid degradation when improving cellulose, hemicellulose and decomposing; Cellulase preparation can activate the secretion of endogenous enzymes, replenishes the deficiency of endogenous enzymes, and endogenous enzymes is adjusted, and guarantees the normal digestion and absorption function of animal, plays diseases prevention, somatotrophic effect; Eliminate ANFs, promote biological healthy growth.Hemicellulose and pectin can produce viscosity solution after being partially soluble in water, increase the viscosity of digest, and endogenous enzymes is caused obstacle, can reduce viscosity and add cellulase, increase the diffusion of endogenous enzymes, improve enzyme and nutrient contact area, promote the good digestion of feed; Cellulase preparation itself is a kind of multienzyme complex of being made up of protease, amylase, pectase and cellulase etc., a kind of product of enzyme can become the substrate of another kind of enzyme in this multienzyme complex is, thereby makes the digestion in the alimentary canal smooth.
(5) pectase: pectic substance is a kind of ANFs in the vegetable raw material, influences the utilization rate of feed.And pectase can effectively destroy pectic substance, promotes the digestion and the absorption of nutritional labeling, and pectase also is a kind of feed enzyme preparation commonly used.
(6) phytase: phytase is the enzyme of phytic acid and phytate thereof in the degraded feed.Have only the phosphorus of 30-40% to be absorbed in the feed of plant origin, and a sizable part is to exist with the nonabsorbable phytate phosphorus form of animal, the phosphorus of this form can not or seldom by the simple stomach animal use.Simultaneously, phytic acid and phytate can with multiple divalent minerals element chelating, form chelating agent influence the micro-digestibility and utilization of animal body, influence the utilization of bivalent cation mineral element, cause the waste of other mineral matter elements such as feed cost increase, phosphorus, cause environmental pollution.Under acid and condition of neutral pH, phytate can combine with protein or amino acid, forms phytate-protein or phytate-amino acid complex, reduces protein and amino acid whose Effective interest rate.In feed, add the benefit of phytase: phytic acid and phytate can be hydrolyzed into inositol and phosphate, absorb, thereby the utilization rate and the mineralization of skeleton degree of raising phosphorus reduce the interpolation of external source phosphorus in the diet, have reduced the waste of feed for animal; Improve the utilization rate of other mineral matter elements such as calcium, magnesium, copper, zinc, reduced discharging wastes, alleviated the threat of environmental pollution; Add phytase in the diet, can make the phytate hydrolysis, the amino acid and the protein of release and phytate chelating make phytic acid reduce protein or amino acid whose inhibitory action, improve protein and amino acid whose utilization rate.
In feeds such as laying hen, fryer, sucking pig, piglet, growth pig and aquatic livestock, add the plurality of enzymes compound formulation, can improve the digestive utilization ratio of feed, promote absorbing of nutriment, help solving the deficiency of China's feed resource, can make full use of the feedstuff that some contain ANFs, therefore more and more come into one's own.
Aspect sucking pig, the feed complex enzyme product that Xie Bifeng (2006) adopts aspergillus niger SL2111 fermenting and producing to go out high enzyme unit alive carries out carrying out feeding experiment on the wean sucking pig.Feeding result through 21 days shows: addition is that 0.1% test group feedstuff-meat ratio is 1.75, reduces by 26.9% than blank group respectively; Addition is that 0.1% test group daily gain is the 0.70kg/ head, improves 25.0% than control group, shows good feeding effect.Song Lianxi etc. add 0.1% pig weanling pig and use complex enzyme formulation, and the result shows that the average daily gain of weanling pig significantly improves 6%, and price of deed ratio has significantly improved 5.6%, and every kilogram of weightening finish feed cost reduces by 0.14 yuan, and economic benefit has improved 4.4%.
Summary of the invention
The purpose of this invention is to provide a kind of piglet complex enzyme preparation for feeding.
Piglet complex enzyme preparation for feeding provided by the present invention comprises following seven kinds of enzymes: acid protease, amylase, zytase, 1,4 beta-glucanase, cellulase, pectase and phytase; The enzyme activity ratio of described seven kinds of enzymes was followed successively by 1: 1: (8-10): (3.3-4): 1: (0.5-0.55): (0-0.02).
Wherein, in every gram piglet complex enzyme preparation for feeding, the enzyme activity of acid protease is that 2000-15000U, diastatic enzyme activity are that the enzyme activity of 2000-15000U, zytase is that the enzyme activity of 20000-120000U, 1,4 beta-glucanase is that the enzyme activity of 8000-50000U, cellulase is that the enzyme activity of 2000-15000U, pectase is that the enzyme activity of 1000-8000U, phytase is 0-40U.
When this complex enzyme formulation was plain edition, enzyme preparation specifically composed as follows: the enzyme activity of acid protease was that 2000U, diastatic enzyme activity are that the enzyme activity of 2000U, zytase is that the enzyme activity of 20000U, 1,4 beta-glucanase is that the enzyme activity of 8000U, cellulase is that the enzyme activity of 2000U, pectase is that the enzyme activity of 1000U, phytase is 40U in every gram piglet complex enzyme preparation for feeding.
When feeding piglet with the plain edition complex enzyme formulation, its addition in piglet diet can be 0.5 ‰-1 ‰.
When this complex enzyme formulation when being reinforced, enzyme preparation specifically composed as follows: in every gram piglet complex enzyme preparation for feeding, the enzyme activity of acid protease is that 15000U, diastatic enzyme activity are that the enzyme activity of 15000U, zytase is that the enzyme activity of 120000U, 1,4 beta-glucanase is that the enzyme activity of 50000U, cellulase is that the enzyme activity of 15000U, pectase is 8000U.
When feeding piglet with the plain edition complex enzyme formulation, its addition in piglet diet can be 0.1 ‰-0.15 ‰.
The design principle of complex enzyme formulation provided by the invention is: piglet digestive system development imperfection, the digestive ferment hyposecretion, cause indigestion, nutriment can not fully be digested by endogenous enzymes, and the nutrition that is not digested arrives the enteron aisle back segment and also easily caused trophism diarrhea by the harmful microorganism utilization.Ablactation stress is that trypsase and diastatic secretion further descend, and the digestion power reduction to feed causes diarrhoea easily, thereby makes growth retardation.At above situation, digestive ferment has been carried out the emphasis reinforcement, fully add protease and amylase, to remedy endogenous digestive ferment deficiency, guarantee the digestion and the absorption of feed nutrient, promote growth in piglets.Add non-starch polysaccharide enzyme, the SNSP in the piglet diet of can fully degrading, reduce the chyme viscosity in the enteron aisle, broken plant cell wall, decomposing SNSP is that animal can utilize sugar, and makes the protein that wraps up in the cell membrane, and nutrients such as starch and fat discharge, promote the absorption of feed nutrition material, improve feedstuff metabolisable energy content.
The beneficial effect of complex enzyme formulation of the present invention is:
1) various ANFs in the degraded feed reduce enteron aisle chyme viscosity, improve feedstuff metabolisable energy;
2) improve the digestibility of piglet to protein and starch;
3) reduce harmful microbe excessive multiplication in the enteron aisle, reduce the damage of intestines wall, reconcile the intestinal microecology balance;
4) improve the piglet survival rate, premunition reduces diarrhea rate, improves the whole uniformity; Promote weight gain of piglets, reduce aquaculture cost.
The specific embodiment
The invention will be further described below by specific embodiment, but the present invention is not limited thereto.
Experimental technique described in the following embodiment if no special instructions, is conventional method; Described material if no special instructions, all can obtain from commercial channels.
Cellulase (forming) by circumscribed β-1,4 dextranase, inscribe β-1,4 dextranase and β-1,4 glucuroide, available from Zhaodong Sun Shine Enzyme Co., Ltd., production code member is 203201; Acid protease, amylase, pectase, 1,4 beta-glucanase, zytase, phytase are all from Beijing Challenge Biotechnologies Co., Ltd., and production code member is respectively 1309,537,860,1342,816 and 1325.
Embodiment 1, the feeding plain edition complex enzyme formulation of piglet and reinforced complex enzyme formulation and feeding effect thereof
The prescription of complex enzyme formulation sees Table 1.
| Complex enzyme formulation is formed | Plain edition (U/g) | Reinforced (U/g) |
| Acid protease | 2000 | 15000 |
| Amylase | 2000 | 15000 |
| Zytase | 20000 | 120000 |
| 1,4 beta-glucanase | 8000 | 50000 |
| Cellulase | 2000 | 15000 |
| Pectase | 1000 | 8000 |
| Phytase | 40 | - |
The feeding effect test:
120 of health " Du * length * big " three way cross weanling pigs are selected in test for use, are divided into 5 groups at random, every group of 4 repetitions, and each repeats 6.The control group fed basal diet, the conventional raising and management.Pre-feed period is 7 days, and the formal phase is 28 days.
The experimental design scheme is as follows:
| Group | Handle |
| Control group | Basal diet |
| Test group 1 | Basal diet+0.5 ‰ plain edition complex enzyme formulations |
| Test group 2 | Basal diet+1 ‰ plain edition complex enzyme formulations |
| Test group 3 | The reinforced complex enzyme formulation in basal diet+0.1 ‰ |
| Test group 4 | Basal diet+0.15 ‰ reinforced complex enzyme formulation |
The composition of experimental basis daily ration (%): corn 65; Dregs of beans 18; Wheat bran 6; Rice bran 6; Fish meal 1; Premix 4.
Trophic level: digestible energy (MJ/kg) 13.03; Crude protein (%) 16.00; Calcium (%) 0.66; Phosphorus (%) 0.55; Lysine (%) 0.79; Methionine+cystine (%) 0.58.
According to the ratio in the design complex enzyme formulation is joined in the basal diet feed and to mix, use as mash feed, or make particulate material with pelleter and use.
Result of the test is as follows:
| Project | Control group | Test 1 group | Test 2 groups | Test 3 groups | Test 4 groups |
| Daily gain/g | 342±49 Bc | 377±42 ABb | 389±52 ABa | 383±46 ABa | 396±38 Aa |
| Daily ingestion amount/g | 527±68 b | 539±63 ab | 544±62 ab | 540±58 ab | 550±62 a |
| The material anharmonic ratio | 1.54±0.13 B | 1.43±0.14 A | 1.40±0.12 A | 1.41±0.16 A | 1.39±0.12 A |
| Diarrhea rate % | 2.28±0.71 B | 1.37±0.10 Ab | 1.23±0.12 Aa | 1.21±0.21 Aa | 1.14±0.08 Aa |
The different capitalizations of shoulder mark are represented difference extremely significantly (P＜001) among the colleague, different lowercase alphabet differentials different significantly (P＜005).
Confirm that by practical application effect piglet complex enzyme preparation for feeding of the present invention can make the piglet daily gain improve 10%-16%, feedstuff-meat ratio reduces 7-10%, and diarrhea rate reduces 40-50%.
Claims (6)
1. a piglet complex enzyme preparation for feeding comprises following seven kinds of enzymes: acid protease, amylase, zytase, 1,4 beta-glucanase, cellulase, pectase and phytase; The enzyme activity ratio of described seven kinds of enzymes was followed successively by 1: 1: (8-10): (3.3-4): 1: (0.5-0.55): (0-0.02).
2. piglet complex enzyme preparation for feeding according to claim 1, it is characterized in that: in every gram piglet complex enzyme preparation for feeding, the enzyme activity of acid protease is that 2000-15000U, diastatic enzyme activity are that the enzyme activity of 2000-15000U, zytase is that the enzyme activity of 20000-120000U, 1,4 beta-glucanase is that the enzyme activity of 8000-50000U, cellulase is that the enzyme activity of 2000-15000U, pectase is that the enzyme activity of 1000-8000U, phytase is 0-40U.
3. piglet complex enzyme preparation for feeding according to claim 2, it is characterized in that: in every gram piglet complex enzyme preparation for feeding, the enzyme activity of acid protease is that 2000U, diastatic enzyme activity are that the enzyme activity of 2000U, zytase is that the enzyme activity of 20000U, 1,4 beta-glucanase is that the enzyme activity of 8000U, cellulase is that the enzyme activity of 2000U, pectase is that the enzyme activity of 1000U, phytase is 40U.
4. piglet complex enzyme preparation for feeding according to claim 2, it is characterized in that: in every gram piglet complex enzyme preparation for feeding, the enzyme activity of acid protease is that 15000U, diastatic enzyme activity are that the enzyme activity of 15000U, zytase is that the enzyme activity of 120000U, 1,4 beta-glucanase is that the enzyme activity of 50000U, cellulase is that the enzyme activity of 15000U, pectase is 8000U.
5. the method for the piglet of feeding comprises: add the described piglet complex enzyme preparation for feeding of 0.5 ‰-1 ‰ claims 3 in the piglet diet feed, adopt the mixed feed piglet of feeding.
6. the method for the piglet of feeding comprises: add the described piglet complex enzyme preparation for feeding of 0.1 ‰-0.15 ‰ claims 4 in the piglet diet feed, adopt the mixed feed piglet of feeding.
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Application publication date: 20110713 |