CN102640858A - Multi-efficacy composite enzyme preparation for feed - Google Patents
Multi-efficacy composite enzyme preparation for feed Download PDFInfo
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- CN102640858A CN102640858A CN201210107415XA CN201210107415A CN102640858A CN 102640858 A CN102640858 A CN 102640858A CN 201210107415X A CN201210107415X A CN 201210107415XA CN 201210107415 A CN201210107415 A CN 201210107415A CN 102640858 A CN102640858 A CN 102640858A
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Abstract
The invention discloses a multi-efficacy composite enzyme preparation for feed. The multi-efficacy composite enzyme preparation comprises the following enzymes in percentage by weight: 30 to 40 percent of xylanase, 10 to 20 percent of beta-glucanase, 15 to 20 percent of mannose and 30 to 40 percent of glucose oxidase. Compared with the prior art, the product of the multi-effect composite enzyme preparation has the following advantage that the influence on the animal production performance is improved by adopting a mode of improving the nutrient digestive utilization and relieving anti-nutrition factors. In addition, the glucose oxidase consumes O2 in the process of acting with glucose, anaerobic environment can be more easily formed in a digestive tract, meanwhile, gluconic acid is generated for promoting the propagation of anaerobic bacteria, and the effects of acidulants and probiotics are realized. The intestinal tract acid digestion environment is sufficiently improved, the growth and the propagation of the germs in the intestinal tract are controlled, the completeness of the intestinal tract epidermal cells is protected, and the intestinal tract flora ecological balance is regulated.
Description
Technical field
What the present invention relates to is a kind of many effects complex enzyme preparation for feeding.
Background technology
Fodder enzyme preparation is broadly divided into digestive ferment and non-digestive ferment two big classes.Non-digestive ferment is meant that animal self can not be secreted into the enzyme in the alimentary canal, and this fermentoid can digest material or some ANFs of degrading that animal self can not digest, and mainly contains cellulase, zytase, 1,4 beta-glucanase, phytase, pectase etc.Digestive ferment is meant amylase, protease and the lipase etc. that animal self can secrete.The feed complex enzyme effect of using in the market is mainly the deficiency and the ANFs that helps in the animal degraded feed that young animal replenishes endogenous enzymes.Enzyme preparation great majority commonly used are hydrolase; Difference by the effect substrate can be divided into amylase, protease, lipase, pectase, zytase, 1,4 beta-glucanase, cellulase, phytase etc., along with the increase of unconventional feedstuff use amount; Non-starch polysaccharide enzyme uses more and more general in daily ration; Can break the constraint to nutrient such as cellulose in the cell membrane, hemicellulose and pectin on the one hand, let digestive ferment contact feed nutrient rapidly fully, nutriment is utilized better; Improve the feed conversion effect, alleviate environmental pollution.To enlarging the feedstuff range of choice, develop new feedstuff resource and play a part positive simultaneously.Feed mold has brought great loss to aquaculture in recent years; Many Feed Manufacturing producer has taked a series of measure; Such as having added mould inhibitor, mould bacteria adsorbent, taken off mould dose etc., the growth that mould inhibitor can only mould fungus inhibition can't be removed the mycotoxin that has produced in the feed.And add mold toxin sorbent or inorganic agent in the feed, and can only be effective to the feed of slight pollution mycotoxin, the serious and mouldy feed of moderate does not have effect, can only discard, and forbids feeding.Toxin such as adsorbents such as manna oligosacchride ability adsorption of aflatoxin and zearalenone have unique mildew-resistant to control mould effect, and complementary therapeutic action, but it is higher to add cost, and many producers are difficult to accept.In addition; Along with people's is to antibiotic residual improve that influences human health and influence the understanding of environment in animal product; It is increasingly high to forbid adding antibiotic cry in the feed, seeks to replace the problem that antibiotic green product is the urgent solution of present feedstuff industry.Based on above situation, developing a kind of many effects complex enzyme preparation for feeding is a kind of good idea.
Summary of the invention
The object of the invention is exactly for the defective that overcomes above-mentioned prior art existence a kind of feed digestibility and conversion ratio of improving to be provided, and reduces the toxic action of mould and toxin, improves the many effects complex enzyme preparation for feeding and the manufacturing approach of animal disease resistant ability.
The object of the invention can be realized through following technical scheme:
A kind of many effects complex enzyme preparation for feeding, it comprises following enzyme and proportioning, percentage by weight is: zytase 30-40%, 1,4 beta-glucanase 10-20%, mannase 15-20% and glucose oxidase 30-40%.
Processing step: get the zytase 30-40% of wooden mould fermenting and producing, the 1,4 beta-glucanase 10-20% of trichoderma reesei fermenting and producing, the glucose oxidase 30-40% that the mannase 15-20% of Pichia pastoris fermenting and producing and fermentation of bacillus subtilis are produced.Mix and form.
Compared with prior art, the present invention has the following advantages:
1, product of the present invention not only improves the influence to its breeding performonce fo animals through digestibility and utilization that improves nutrient and the mode that alleviates ANFs.And, through glucose oxidase with the glucose mechanism in consume O
2, make alimentary canal more be prone to form anaerobic environment, produce the propagation that gluconic acid impels the anaerobism beneficial bacterium simultaneously, play the effect of acidulant and probio.Substantially improve the intestinal acidity digestive environments, the breeding of control enteron aisle pathogen growth, the protection intestinal epithelial cell is complete, the adjustment gut flora ecological balance.
2, many effects of product complex enzyme preparation for feeding of the present invention can directly suppress multiple moulds such as aspergillus flavus, bread mold, mould, promotes the redox reaction in the liver, and liver assist metabolism toxin can be removed the harm that mould and toxin cause animal well.
3, product main degradation products major part of the present invention is an oligosaccharide, thus have certain disease-resistant and anti-stress effect.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is elaborated.
The animal feeding result of the test of many effects complex enzyme preparation for feeding:
Test one: many effects complex enzyme preparation for feeding is to the influence of newborn piglet production performance:
1.1 the selection of experimental animal
Select 60 of piglets of athletic three way cross (Du * length * big) (public female half and half), the wean of 30 ages in days, average weanling weight 7.93Kg.
1.2 experimental design
60 of 30 age in days weanling pigs are divided into 2 groups of control group and test group at random.Every group 30, establish 5 repetitions, each repeats 6 piglets (public female half and half) is an independent hurdle.The conventional weanling pig basal diet of control group fed, test group add percentage by weight in basal diet be many effects complex enzyme preparation for feeding of 0.3%.This many effects complex enzyme preparation for feeding comprises following enzyme and proportioning, and percentage by weight is: zytase 30%, 1,4 beta-glucanase 20%, mannase 15% and glucose oxidase 35%.
Test divides two stages: in one week of preliminary trial period phase I, second stage is for just trying 28 days phases.
Basal diet is prepared with reference to relevant ideal protein mode standard voluntarily, and trophic level is: metabolizable energy 13.04MJ/kg, CP18.12%; Ca0.7%, P0.58%, available phosphorus 0.31%; Effective lysine 0.89%; Effective methionine 0.25%, effectively methionine+effective cystine 0.50%, effectively threonine 0.60%.
1.3 feeding and management
Test pig is raised at the high bed of same pig house slatted floor, free choice feeding and drinking-water, and feedings and managements such as epidemic prevention, expelling parasite are according to conventional identical carrying out.
1.4 result of the test: duration of test is observed piglet every day and is searched for food and health status and diarrhoea phenomenon; Every piglet empty stomach body weight is calculated average daily gain, average daily ingestion amount, feed conversion rate and diarrhea rate when record daily feed intake, diarrhoea number of times, on-test and end.
Table 1 performance test
| Project | Control group | Test group |
| Starting weight (kg) | 7.94±1.35a | 7.92±1.32a |
| End heavy (kg) | 17.09±2.48 | 19.35±2.20 |
| Daily gain (g) | 316.6±37.1a | 395.7±36.8Ab |
| Daily ingestion amount (g) | 626.7±43.8a | 648.9±34.8a |
| Feedstuff-meat ratio | 1.98±0.03a | 1.64±0.08Ab |
| Diarrhea rate (%) | 7.53±1.88a | 3.31±1.27b |
Annotate: the identical person of colleague's lowercase representes difference not remarkable (P＞0.05); Colleague's capitalization is identical and lowercase difference person representes significant difference (P＜0.05); The different persons of upper and lower case letter represent difference extremely significantly (P＜0.01).
Test two: studied of the influence of many effects complex enzyme preparation for feeding to production performance of layer chicken.The single factor experiment design is adopted in test, selects 180 of the blue brown laying hens in 26 seas in age in week, is divided into 5 groups at random, every group of 4 repetitions, and each repeats 9 chickens.Test I, II, III, IV, V group are added percentage by weight 0, many effects complex enzyme preparation for feeding of 0.2%, 0.3%, 0.4%, 0.5% respectively in basal diet.This many effects complex enzyme preparation for feeding comprises following enzyme and proportioning, and percentage by weight is: zytase 40%, 1,4 beta-glucanase 10%, mannase 20% and glucose oxidase 30%.
Raise one week of phase in advance, experimental period was 6 weeks, and statistics is respectively organized production performance, calculated laying rate, feedstuff-egg ratio, average egg weight, average daily feed intake.
Result of the test shows: add many effects complex enzyme preparation for feeding in the layer diets and can improve production performance and economic benefit.The laying rate of II, III, IV, V group has improved 11.01% (P＞0.05) than control group respectively, 12.34% (P＞0.05), 16.06% (P＜0.01), 13.79% (P＜0.05); Many effects complex enzyme preparation for feeding interpolation group has significantly reduced soft broken lopsided egg rate, and broken (soft) egg rate of II, III, IV, V group reduces by 32.77 (P＞0.05), 33.45 (P＞0.05), 49.66 (P＜0.05), 33.11 (P＞0.05) than control group respectively; 0.4% and the complex enzyme preparation for feeding interpolation of effect more than 0.5% group significantly improved feed conversion rate (P＜0.05); Many effects complex enzyme preparation for feeding interpolation group has significantly reduced death rate, with 0.4% and 0.5% interpolation group effect the most obviously (P＜0.01); Average egg weight between each test group, a daily material consumption and day egg production difference not significantly (P＞0.05) only; Many effects complex enzyme preparation for feeding interpolation group has all improved economic benefit, and 0.3% interpolation group obtains the highest economic benefit (P＜0.05).
Should be understood that, concerning those of ordinary skills, can improve or conversion, and all these improvement and conversion all should belong to the protection domain of accompanying claims of the present invention according to above-mentioned explanation.
Claims (1)
1. effect complex enzyme preparation for feeding more than a kind, it is characterized in that: it comprises following enzyme and proportioning, percentage by weight is: zytase 30-40%, 1,4 beta-glucanase 10-20%, mannase 15-20% and glucose oxidase 30-40%.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103493987A (en) * | 2013-09-29 | 2014-01-08 | 广东大华农动物保健品股份有限公司 | Water-soluble complex enzyme preparation for preventing and treating diarrhea and promoting growth of livestock and poultry and preparation method thereof |
| CN103892135A (en) * | 2014-04-15 | 2014-07-02 | 湖南农业大学 | Piglet feed complex enzyme preparation and production method and application thereof |
| CN103892134A (en) * | 2014-04-15 | 2014-07-02 | 湖南农业大学 | Compound enzyme preparation for hen feed as well as production method and application thereof |
| CN104605165A (en) * | 2015-03-02 | 2015-05-13 | 武汉新华扬生物股份有限公司 | Compound feed additive replacing feed antibiotics in piglet daily diet, and applications of compound feed additive |
| CN105192312A (en) * | 2015-09-18 | 2015-12-30 | 河北农业大学 | Pig feed additive and preparation method thereof |
| CN107927382A (en) * | 2017-10-30 | 2018-04-20 | 山东弘德农牧科技有限公司 | A kind of complex enzyme preparation for feeding and preparation method and application |
| CN109645238A (en) * | 2018-12-11 | 2019-04-19 | 北京盛拓达生物技术有限公司 | It is a kind of promote animal diets cellulose effective use enzyme preparation product and its application |
| CN110946215A (en) * | 2019-12-11 | 2020-04-03 | 中牧实业股份有限公司 | Antibacterial complex enzyme preparation and application thereof |
| CN111280341A (en) * | 2020-03-25 | 2020-06-16 | 武汉新华扬生物股份有限公司 | Compound enzyme preparation and compound feed for preventing and treating necrotic enteritis of poultry |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103493987A (en) * | 2013-09-29 | 2014-01-08 | 广东大华农动物保健品股份有限公司 | Water-soluble complex enzyme preparation for preventing and treating diarrhea and promoting growth of livestock and poultry and preparation method thereof |
| CN103493987B (en) * | 2013-09-29 | 2015-08-26 | 广东大华农动物保健品股份有限公司 | A kind ofly prevent and treat water-soluble complex enzyme preparation of livestock and poultry diarrhea and growth promoting effects and preparation method thereof |
| CN103892135A (en) * | 2014-04-15 | 2014-07-02 | 湖南农业大学 | Piglet feed complex enzyme preparation and production method and application thereof |
| CN103892134A (en) * | 2014-04-15 | 2014-07-02 | 湖南农业大学 | Compound enzyme preparation for hen feed as well as production method and application thereof |
| CN103892134B (en) * | 2014-04-15 | 2015-09-16 | 湖南农业大学 | A kind of egg feedstuff complex enzyme formulation and production method thereof and application |
| CN103892135B (en) * | 2014-04-15 | 2015-10-21 | 湖南农业大学 | A kind of the feed for piglet complex enzyme formulation and production method thereof and application |
| CN104605165A (en) * | 2015-03-02 | 2015-05-13 | 武汉新华扬生物股份有限公司 | Compound feed additive replacing feed antibiotics in piglet daily diet, and applications of compound feed additive |
| CN105192312A (en) * | 2015-09-18 | 2015-12-30 | 河北农业大学 | Pig feed additive and preparation method thereof |
| CN107927382A (en) * | 2017-10-30 | 2018-04-20 | 山东弘德农牧科技有限公司 | A kind of complex enzyme preparation for feeding and preparation method and application |
| CN109645238A (en) * | 2018-12-11 | 2019-04-19 | 北京盛拓达生物技术有限公司 | It is a kind of promote animal diets cellulose effective use enzyme preparation product and its application |
| CN110946215A (en) * | 2019-12-11 | 2020-04-03 | 中牧实业股份有限公司 | Antibacterial complex enzyme preparation and application thereof |
| CN111280341A (en) * | 2020-03-25 | 2020-06-16 | 武汉新华扬生物股份有限公司 | Compound enzyme preparation and compound feed for preventing and treating necrotic enteritis of poultry |
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Application publication date: 20120822 |