CN103892134B - A kind of egg feedstuff complex enzyme formulation and production method thereof and application - Google Patents

A kind of egg feedstuff complex enzyme formulation and production method thereof and application Download PDF

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CN103892134B
CN103892134B CN201410149815.6A CN201410149815A CN103892134B CN 103892134 B CN103892134 B CN 103892134B CN 201410149815 A CN201410149815 A CN 201410149815A CN 103892134 B CN103892134 B CN 103892134B
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enzyme
alive
egg feedstuff
complex enzyme
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CN103892134A (en
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陈清华
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Hunan Debang Animal Husbandry Co.,Ltd.
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Hunan Agricultural University
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Abstract

The invention discloses a kind of egg feedstuff complex enzyme formulation and production method thereof and application.The enzyme of this complex enzyme formulation is lived and is consisted of: zytase 12000-15000U/g,-dextranase 1000-1200U/g, cellulase 500-600U/g,-mannase 500-750U/g, pectase 500-750U/g, acid protease 3000-4000U/g, neutral proteinase 1000-1200U/g, mesophilicα-diastase 750-1000U/g, fungal alpha-amylase 1500-2000U/g, carbohydrase 2000-2500U/g.The cornstarch of the mass parts such as this complex enzyme formulation employing and mixture of calcium sulfate are as diluent.The addition of this invention product in complete compound feed for laying hen per ton is 150-200 gram.

Description

A kind of egg feedstuff complex enzyme formulation and production method thereof and application
Technical field
The present invention relates to a kind of egg feedstuff complex enzyme formulation and production method thereof and application.
Background technology
Current China egg total output and laying hen amount of livestock on hand rank the first in the world, and keeping quick growth trend over nearly 30 years always.Along with the development of laying hen industry, by pursuing merely the traditional concept that laying hen high yield is exactly high benefit, to should production cost be reduced, improving feeding and management and epidemic prevention and control level again, paying attention to feedstuff-egg ratio, pursue the Concept Change of high benefit comprehensively.How to reduce feed use cost, feed producer adjusts feed formula in all directions, uses some unconventional raw materials (feedstuff beyond corn, dregs of beans) such as cotton dregs, the dish dregs of rice, corn processing accessory substance, fiber feedstuff, secondary powder in a large number.Due in unconventional feed containing, for example ANFs such as xylan, cellulose ,-glucans, inhibit laying hen to the absorption and digestion of nutriment, thus affect its egg laying performance.In order to reduce the negative effect of ANFs, in feed, usually add feed enzyme preparation in production to improve the utilization rate of nutriment.But, egg feedstuff complex enzyme formulation product on market is very different, for solving the anti-oxidant action of SNSP in layer diets and the enzyme preparation product prepared mostly, often ignore the digestive utilization ratio of the conventional nutrients in the alimentary canal physilogical characteristics of laying hen and corn, dregs of beans raw material, therefore, the result of use of current enzyme preparation is all undesirable, does not reach and can improve Layer Production Performance, can reduce again the effect of Feed Manufacturing cost and use cost.Developing the inexorable trend that a kind of new and effective egg feedstuff complex enzyme formulation is feedstuff industry development, is also the inevitable requirement of laying hen industry market competition.
Summary of the invention
The object of this invention is to provide and a kind ofly can improve egg feedstuff digestibility, reduce feedstuff-egg ratio, extend laying period, promote laying rate, improve the egg feedstuff complex enzyme formulation of feeding environment and production method thereof and application.
Object of the present invention can be achieved through the following technical solutions:
A kind of egg feedstuff complex enzyme formulation, its enzyme is lived and is consisted of: zytase 12000 U/g-15000U/g;-dextranase 1000 U/g-1200U/g; Cellulase 500 U/g-600U/g;-mannase 500 U/g-750U/g; Pectase 500 U/g-750U/g; Acid protease 3000 U/g-4000U/g; Neutral proteinase 1000 U/g-1200U/g; Mesophilicα-diastase 750 U/g-1000U/g; Fungal alpha-amylase 1500 U/g-2000U/g; Carbohydrase 2000 U/g-2500U/g.
A kind of egg feedstuff complex enzyme formulation, by weight percentage, this complex enzyme formulation is formulated by following component: zytase 24-30 part of enzyme 50,000 U/g alive, enzyme 50,000 U/g alive-dextranase 2-2.4 part, cellulase 2.5-3 part of enzyme 20,000 U/g alive, enzyme 50,000 U/g alive-mannase 1-1.5 part, pectase 1.7-2.5 part of enzyme 30,000 U/g alive, acid protease 6-8 part of enzyme 50,000 U/g alive, neutral proteinase 1-1.2 part of enzyme 100,000 U/g alive, mesophilicα-diastase 37.5-50 part of enzyme 2000U/g alive, fungal alpha-amylase 1.5-2 part of enzyme 100,000 U/g alive, carbohydrase 2-2.5 part of enzyme 100,000 U/g alive, diluent 1-5 part.
The cornstarch of the mass parts such as described diluent is and mixture of calcium sulfate.
The production method of described egg feedstuff complex enzyme formulation comprises the following steps:
1), get enzyme and to live zytase 24-30 part of 50,000 U/g, enzyme live 50,000 U/g-dextranase 2-2.4 part, enzyme is lived cellulase 2.5-3 part of 20,000 U/g, enzyme 50,000 U/g alive-mannase 1-1.5 part, pectase 1.7-2.5 part of enzyme 30,000 U/g alive, mixes, as No. 1, pre-mixing agent material;
2), get enzyme and to live acid protease 6-8 part of 50,000 U/g, enzyme is lived neutral proteinase 1-1.2 part of 100,000 U/g, mixes, as No. 2, pre-mixing agent material;
3), get enzyme and to live mesophilicα-diastase 37.5-50 part of 2000U/g, enzyme is lived fungal alpha-amylase 1.5-2 part of 100,000 U/g, and enzyme is lived carbohydrase 2-2.5 part of 100,000 U/g, mixes, as No. 3, pre-mixing agent material;
4), get No. 1, pre-mixing agent material, No. 2 material, No. 3 each portions of material, the cornstarch of the mass parts such as interpolation and mixture of calcium sulfate 1-5 part, mix, to obtain final product.
The addition of described egg feedstuff complex enzyme formulation in complete compound feed for laying hen is 150-200 gram/ton.Be preferably 200 grams/ton.
Described zytase is produced by Trichoderma Viride, described-dextranase is by trichoderma reesei fermenting and producing, described cellulase is by trichoderma reesei fermenting and producing, described-mannase is by Pichia pastoris fermenting and producing, described pectase is produced by fermentation of Aspergillus niger, described acid protease is produced by fermentation of Aspergillus niger, described neutral proteinase is produced by fermentation of bacillus subtilis, described mesophilicα-diastase is produced by fermentation of bacillus subtilis, described fungal alpha-amylase is by aspergillus oryzae fermenting and producing, and described carbohydrase is produced by fermentation of Aspergillus niger.
The present invention is based on the physilogical characteristics of laying hen, select the enzyme preparation such as amylase, protease that applicable laying hen gastrointestinal tract environment plays a role, the effect of strengthening digestibility enzyme preparation.Simultaneously, take into full account feedstuff characteristic conventional in current feed formula for laying hen, select dissimilar amylase (mesophilicα-diastase, fungal alpha-amylase, carbohydrase), protease (acid protease, neutral proteinase) and Non-starch Polysaccharides Enzyme (zytase, cellulase, mannase, pectase,-dextranase) carry out compatibility, feed nutrient digestibility can be improved, improve laying rate of laying hen, reduce feedstuff-egg ratio, feedstuff range of choice can be expanded again, develop new feedstuff resource, reach the object reducing Feed Manufacturing cost and use cost.
Advantage of the present invention:
1, product of the present invention contains cellulase ,-dextranase, pectase, zytase ,-mannase, compatibility science, the cellulose in feedstuff, hemicellulose, the degradation rate of SNSP and digestibility can be improved, for widening Feed selection scope, expansion unconventional water resources use amount provides possibility, and then reaches the object reducing Feed Manufacturing cost.The Non-starch Polysaccharides Enzyme selected is the enzyme preparation of originated from fungus, based on endo-type enzyme preparation, a large amount of compound sugar is produced after substrate specificity, as the oligosaccharide such as xylo-oligosaccharide, Oligomeric manna sugar, promote the beneficial microbe propagation such as laying hen intestinal bifidobacteria, significantly improve the healthy body condition of laying hen.
2, product of the present invention contains the amylase (mesophilicα-diastase of three types, fungal alpha-amylase, carbohydrase), the amylose, amylopectin, dextrin, maltose etc. that can fully degrade in feed, greatly improve the digestibility of starch in feed, improve fodder energy utilization rate, thus improve laying rate, reduce feedstuff-egg ratio.
3, product of the present invention contains the protease (acid protease of two types, neutral proteinase), the alcohol soluble protein etc. that the globulin in beans seed of fully degrading source, the albumin in grouts source, paddy reality and accessory substance are originated, greatly improve the digestibility of protein in feed, improve Amino Acid Absorption utilization rate, thus appropriateness improves egg size, reduce feedstuff-egg ratio.
4, the present invention selects calcium sulfate and cornstarch to make diluent, wherein, calcium sulfate appropriateness can improve the heat resistance of enzyme preparation in feed preparation, in animal body simultaneously, free calcium ion can activate or promote the reaction of enzyme, improves the action effect of enzyme preparation; Cornstarch while guarantee mixture homogeneity, can digestedly can utilize again in animal body.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment one: 1, get enzyme and to live the zytase 24 parts of 50,000 U/g, enzyme live 50,000 U/g-dextranase 2 parts, enzyme is lived the cellulase 2.5 parts of 20,000 U/g, enzyme 50,000 U/g alive-mannase 1 part, the pectase 1.7 parts of enzyme 30,000 U/g alive, mixes, as No. 1, pre-mixing agent material.
2, get the acid protease 6 parts of enzyme 50,000 U/g alive, the neutral proteinase 1 part of enzyme 100,000 U/g alive, mixes, as No. 2, pre-mixing agent material.
3, get the mesophilicα-diastase 37.5 parts of enzyme 2000U/g alive, the fungal alpha-amylase 1.5 parts of enzyme 100,000 U/g alive, 2 parts, the carbohydrase of enzyme 100,000 U/g alive, mixes, as No. 3, pre-mixing agent material.
4, get No. 1, pre-mixing agent material, No. 2 material, No. 3 each portions of material, the cornstarch of the mass parts such as interpolation and mixture of calcium sulfate 1 part, mix, with 1 kilogram, 2 kilograms, the packaging bags of 10 kilograms or 20 kilograms specifications, is novel laying hen feed complex enzyme formulation product.
5, using method: the addition of this invention product in complete compound feed for laying hen per ton is 150 grams.
Embodiment two: 1, get enzyme and to live the zytase 26 parts of 50,000 U/g, enzyme live 50,000 U/g-dextranase 2.2 parts, enzyme is lived the cellulase 2.75 parts of 20,000 U/g, enzyme 50,000 U/g alive-mannase 2.25 parts, the pectase 2.1 parts of enzyme 30,000 U/g alive, mixes, as No. 1, pre-mixing agent material.
2, get the acid protease 7 parts of enzyme 50,000 U/g alive, the neutral proteinase 1.1 parts of enzyme 100,000 U/g alive, mixes, as No. 2, pre-mixing agent material.
3, get the mesophilicα-diastase 43.75 parts of enzyme 2000U/g alive, the fungal alpha-amylase 1.75 parts of enzyme 100,000 U/g alive, 2.25 parts, the carbohydrase of enzyme 100,000 U/g alive, mixes, as No. 3, pre-mixing agent material.
4, get No. 1, pre-mixing agent material, No. 2 material, No. 3 each portions of material, the cornstarch of the mass parts such as interpolation and mixture of calcium sulfate 3 parts, mix, with 1 kilogram, 2 kilograms, the packaging bags of 10 kilograms or 20 kilograms specifications, is novel laying hen feed complex enzyme formulation product.
5, using method: the addition of this invention product in complete compound feed for laying hen per ton is 175 grams.
Embodiment three: 1, get enzyme and to live the zytase 30 parts of 50,000 U/g, enzyme live 50,000 U/g-dextranase 2.4 parts, enzyme is lived the cellulase 3 parts of 20,000 U/g, enzyme 50,000 U/g alive-mannase 1.5 parts, the pectase 2.5 parts of enzyme 30,000 U/g alive, mixes, as No. 1, pre-mixing agent material.
2, get the acid protease 8 parts of enzyme 50,000 U/g alive, the neutral proteinase 1.2 parts of enzyme 100,000 U/g alive, mixes, as No. 2, pre-mixing agent material.
3, get the mesophilicα-diastase 50 parts of enzyme 2000U/g alive, the fungal alpha-amylase 2 parts of enzyme 100,000 U/g alive, 2.5 parts, the carbohydrase of enzyme 100,000 U/g alive, mixes, as No. 3, pre-mixing agent material.
4, get No. 1, pre-mixing agent material, No. 2 material, No. 3 each portions of material, the cornstarch of the mass parts such as interpolation and mixture of calcium sulfate 5 parts, mix, with 1 kilogram, 2 kilograms, the packaging bags of 10 kilograms or 20 kilograms specifications, is novel laying hen feed complex enzyme formulation product.
5, using method: the addition of this invention product in complete compound feed for laying hen per ton is 200 grams.
Test: the impact studying egg feedstuff Compound Enzyme Preparation on Performance of Layers of the present invention.Test adopts single factor experiment design, selects the blue grey laying hen 1600 in sea in 26 week age, is divided into 2 groups at random, often organizes 4 repetitions, each repetition 200 chickens.Test group adds 200 grams of novel laying hen feed complex enzyme formulations in control group basal diet.Basal diet bulk raw material formula is: corn 40%, dregs of beans 12%, cotton dregs 5%, fiber feedstuff 15%, DDGS15%, stone flour 8%, calcium monohydrogen phosphate 1%, premix 4%.Raise one week phase in advance, experimental period is 6 weeks, adds up each group of production performance, calculates laying rate, feedstuff-egg ratio, average egg weight, average daily feed intake.
Result of the test shows: add novel laying hen feed complex enzyme formulation in layer diets and can improve production performance and economic benefit.Laying rate, the shell thickness of test group improve 3.79% and 2.38% than control group; Reduce soft shell deformity egg rate and laying hen death rate 0.87% and 0.32%, the feed conversion rate of test group improves 3.25%, significant difference (P<0.05).
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.

Claims (6)

1. an egg feedstuff complex enzyme formulation, is characterized in that, the enzyme of this complex enzyme formulation is lived and consisted of: zytase 12000 U/g-15000U/g;-dextranase 1000 U/g-1200U/g; Cellulase 500 U/g-600U/g;-mannase 500 U/g-750U/g; Pectase 500 U/g-750U/g; Acid protease 3000 U/g-4000U/g; Neutral proteinase 1000 U/g-1200U/g; Mesophilicα-diastase 750 U/g-1000U/g; Fungal alpha-amylase 1500 U/g-2000U/g; Carbohydrase 2000 U/g-2500U/g.
2. an egg feedstuff complex enzyme formulation according to claim 1, it is characterized in that, by weight percentage, this complex enzyme formulation is formulated by following component: zytase 24-30 part of enzyme 50,000 U/g alive, enzyme 50,000 U/g alive-dextranase 2-2.4 part, cellulase 2.5-3 part of enzyme 20,000 U/g alive, enzyme 50,000 U/g alive-mannase 1-1.5 part, pectase 1.7-2.5 part of enzyme 30,000 U/g alive, acid protease 6-8 part of enzyme 50,000 U/g alive, neutral proteinase 1-1.2 part of enzyme 100,000 U/g alive, mesophilicα-diastase 37.5-50 part of enzyme 2000U/g alive, fungal alpha-amylase 1.5-2 part of enzyme 100,000 U/g alive, carbohydrase 2-2.5 part of enzyme 100,000 U/g alive, diluent 1-5 part.
3. a kind of egg feedstuff complex enzyme formulation according to claim 2, is characterized in that, described diluent such as is at cornstarch and the mixture of calcium sulfate of mass parts.
4. the production method of egg feedstuff complex enzyme formulation according to claim 3, is characterized in that, comprise the following steps:
1), get enzyme and to live zytase 24-30 part of 50,000 U/g, enzyme live 50,000 U/g-dextranase 2-2.4 part, enzyme is lived cellulase 2.5-3 part of 20,000 U/g, enzyme 50,000 U/g alive-mannase 1-1.5 part, pectase 1.7-2.5 part of enzyme 30,000 U/g alive, mixes, as No. 1, pre-mixing agent material;
2), get enzyme and to live acid protease 6-8 part of 50,000 U/g, enzyme is lived neutral proteinase 1-1.2 part of 100,000 U/g, mixes, as No. 2, pre-mixing agent material;
3), get enzyme and to live mesophilicα-diastase 37.5-50 part of 2000U/g, enzyme is lived fungal alpha-amylase 1.5-2 part of 100,000 U/g, and enzyme is lived carbohydrase 2-2.5 part of 100,000 U/g, mixes, as No. 3, pre-mixing agent material;
4), get No. 1, pre-mixing agent material, No. 2 material, No. 3 material, the cornstarch of the mass parts such as interpolation and mixture of calcium sulfate 1-5 part, mix, to obtain final product.
5. the application of egg feedstuff complex enzyme formulation according to claim 1 in egg feedstuff, is characterized in that, the addition of this complex enzyme formulation in complete compound feed for laying hen is 150-200 gram/ton.
6. the application of egg feedstuff complex enzyme formulation in egg feedstuff described in claim 1 or 5, is characterized in that, the addition of this complex enzyme formulation in complete compound feed for laying hen is 200 grams/ton.
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