CN112970965B - Sichuan mountain black-bone chicken feed and preparation method thereof - Google Patents

Sichuan mountain black-bone chicken feed and preparation method thereof Download PDF

Info

Publication number
CN112970965B
CN112970965B CN202110308386.2A CN202110308386A CN112970965B CN 112970965 B CN112970965 B CN 112970965B CN 202110308386 A CN202110308386 A CN 202110308386A CN 112970965 B CN112970965 B CN 112970965B
Authority
CN
China
Prior art keywords
parts
complex enzyme
enzyme
black
bone
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202110308386.2A
Other languages
Chinese (zh)
Other versions
CN112970965A (en
Inventor
晏家友
刁慧
李书伟
汤文杰
邝声耀
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sichuan Animal Science Academy
Original Assignee
Sichuan Animal Science Academy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sichuan Animal Science Academy filed Critical Sichuan Animal Science Academy
Priority to CN202110308386.2A priority Critical patent/CN112970965B/en
Priority to AU2021103023A priority patent/AU2021103023A4/en
Publication of CN112970965A publication Critical patent/CN112970965A/en
Application granted granted Critical
Publication of CN112970965B publication Critical patent/CN112970965B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/111—Aromatic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/116—Heterocyclic compounds
    • A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Health & Medical Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physiology (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Birds (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Fodder In General (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention discloses Sichuan mountain black-bone chicken feed and a preparation method thereof, and belongs to the technical field of feeds, wherein the Sichuan mountain black-bone chicken feed comprises the following components in parts by weight: 40-80 parts of corn, 20-30 parts of bean pulp, 5-10 parts of yeast protein, 5-10 parts of corn protein powder, 1-3 parts of soybean oil, 3-5 parts of stone powder, 2-4 parts of stone particles, 1-2 parts of calcium hydrophosphate, 0.1-0.2 part of vitamin premix, 0.1-0.3 part of microelement premix, 2-4 parts of complex microbial inoculum, 1-5 parts of complex enzyme, 1-2 parts of amino acid premix, 0.2-0.4 part of salt and 0.4-0.6 part of bentonite; the reasonable proportion of the compound microbial inoculum and the compound enzyme in the formula improves the nutrient absorption efficiency, enhances the immunity of organisms, reduces the occurrence ratio of diseases, and avoids the use of medicines at the breeding end, thereby ensuring the production of black-bone eggs and black-bone chicken with safe and reliable quality, meeting the consumption demands of people on native eggs and native chicken, and promoting the civil engineering of vegetable baskets.

Description

Sichuan mountain black-bone chicken feed and preparation method thereof
Technical Field
The invention belongs to the technical field of feeds, and particularly relates to a Sichuan mountain black-bone chicken feed and a preparation method thereof.
Background
The Sichuan mountain black-bone chicken belongs to a meat-egg dual-purpose native chicken variety, is bred by locking Sichuan local chicken breeder black-bone chickens, mainly produced in the south of Sichuan and the south of West mountain areas, and has wide distribution and large quantity. The chicken species has the characteristics of wide adaptability, strong disease resistance and stable genetic performance. The Sichuan mountain black-bone chickens generally adopt an ecological stocking mode, and according to detection, daily feed generally only contains 11% -12% of crude protein, if the chickens are fed inadequately, imbalance of nutrition is easily caused, the daily egg yield is low, the meat yield performance is poor, and meanwhile, in the feeding mode, the egg collection difficulty is high. It has been considered that 11% -12% crude protein in the diet of layer chicken is theoretically enough, but in order to obtain better production results, the level of crude protein is actually not lower than 14%.
Moreover, due to the shortage of high-quality protein feed raw materials in China, the large-scale use of unconventional protein raw materials in the Sichuan mountain black-bone chicken feed has great negative influence on the breeding benefit, and meanwhile, the activity of microbial agents and enzyme agents in the existing feed can be reduced along with the extension of time, so that the absorption of crude protein of the feed is unstable, and the production performance and slaughter performance of the Sichuan mountain black-bone chicken are greatly influenced.
At present, the research on feed for Sichuan mountain black-bone chickens is quite lacking, and in order to improve the quality of meat and egg products of Sichuan mountain black-bone chickens and promote the large-scale cultivation production of Sichuan local high-quality chicken varieties, the preparation of feed for Sichuan mountain black-bone chickens, in particular the application of microbial agents and enzyme agents in the feed, is very necessary to develop related research.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide Sichuan mountain black-bone chicken feed and a preparation method thereof.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows:
the Sichuan mountain black-bone chicken feed comprises the following components in parts by weight: 40-80 parts of corn, 20-30 parts of bean pulp, 5-10 parts of yeast protein, 5-10 parts of corn protein powder, 1-3 parts of soybean oil, 3-5 parts of stone powder, 2-4 parts of stone particles, 1-2 parts of calcium hydrophosphate, 0.1-0.2 part of vitamin premix, 0.1-0.3 part of microelement premix, 2-4 parts of complex microbial inoculum, 1-5 parts of complex enzyme, 1-2 parts of amino acid premix, 0.2-0.4 part of salt and 0.4-0.6 part of bentonite.
Further, the composite microbial inoculum comprises any four of saccharomycetes, lactobacillus casei, lactobacillus crispatus and lactobacillus reuteri.
Further, the composite microbial inoculum comprises saccharomycetes, lactobacillus crispatus and lactobacillus reuteri.
Further, the composite microbial inoculum comprises the following components in parts by weight: 4-8 parts of saccharomycetes, 2-4 parts of lactobacillus crispatus and 1-3 parts of lactobacillus reuteri.
Further, the complex enzyme agent comprises complex enzyme I and complex enzyme II; complex enzyme I comprises beta-glucanase, pectinase, xylanase, cellulase, saccharifying enzyme, neutral protease and protective agent, and complex enzyme II comprises trypsin, cellulase, sodium alginate, polyanionic polysaccharide, whey protein isolate and soybean oil.
Further, the mass ratio of the complex enzyme I to the complex enzyme II is 10-15:4-6; the complex enzyme I comprises, by weight, 1-3 parts of beta-glucanase, 1-3 parts of pectase, 1-2 parts of xylanase, 1-2 parts of cellulase, 1-3 parts of saccharifying enzyme, 1-3 parts of neutral protease and 2-3 parts of protective agent, and the complex enzyme II comprises 4-6 parts of trypsin, 2-3 parts of cellulase, 1-2 parts of sodium alginate, 0.5-1 part of polyanionic polysaccharide, 0.5-1 part of whey protein isolate and 0.5-1 part of soybean oil.
Further, the protective agent comprises, by weight, 5-10 parts of disodium glycyrrhizate, 4-6 parts of vanillin, 4-6 parts of sodium chloride, 2-4 parts of stevioside, 2-4 parts of potassium chloride, 1-3 parts of Chalcanthitum, 1-2 parts of resveratrol and 1-2 parts of myo-inositol.
The preparation method of the Sichuan mountain black-bone chicken feed comprises the following steps: weighing the raw materials according to the proportion, uniformly mixing, adding water, uniformly stirring, granulating and air-drying in sequence to obtain the Sichuan mountain black-bone chicken feed.
Further, the preparation method of the complex enzyme I comprises the following steps: mixing disodium glycyrrhizate, vanillin, sodium chloride, stevioside, potassium chloride and Chalcanthitum, pulverizing to 70-80 mesh, placing in 70-80deg.C steam for 3-4 min, rapidly cooling to 20-24deg.C, and standing for 1-2 hr to obtain mixture I; mixing resveratrol and myo-inositol, pulverizing to 30-36 mesh, adding 100-200 parts of 80-95% ethanol solution, maintaining ultrasonic frequency at 30-40deg.C at 200-300W, filtering after 50-80 min, collecting filtrate, heating to remove ethanol, freezing at-10deg.C to-20deg.C for 8-9 hr, and vacuum drying for 3-5 hr to obtain mixture II; and uniformly mixing beta-glucanase, pectinase, xylanase, cellulase, saccharifying enzyme and neutral protease with the mixture I and the mixture II to prepare the compound enzyme I.
Further, the preparation method of the complex enzyme II comprises the following steps: mixing trypsin and cellulase uniformly, then adding sodium alginate and polyanion polysaccharide for coating, and finally adding whey protein isolate and soybean oil for coating after mixing to prepare the compound enzyme II.
Further, the vitamin premix, the trace element premix and the amino acid premix are all prepared from commercially available premix.
The microbial inoculum and the enzyme agent are ideal functional raw materials, daily ration prepared by the microbial inoculum and the enzyme agent is examined, the influence of the microbial inoculum and the enzyme agent on the production performance and slaughter performance of the Sichuan mountain black-bone chicken is researched, and test basis is provided for fully playing the production potential of the Sichuan mountain black-bone chicken and improving the production performance of the Sichuan mountain black-bone chicken.
The invention has the beneficial effects that:
according to the invention, the compound feed containing a plurality of composite bacterial agents and composite enzyme agents is fed to the Sichuan mountain black-bone chickens, so that the use of unconventional feed resources is reduced, the digestibility of protein is improved, the raw materials of protein feed are saved, and the cultivation benefit is improved; the feed adopting the formula of the invention can be used for feeding Sichuan mountain black-bone chickens, so that the laying rate and the meat yield of the black-bone chickens can be obviously improved; the reasonable proportion of the compound microbial inoculum and the compound enzyme in the formula improves the nutrient absorption efficiency, enhances the immunity of organisms, reduces the occurrence ratio of diseases, and avoids the use of medicines at the breeding end, thereby ensuring the production of black-bone eggs and black-bone chicken with safe and reliable quality, meeting the consumption demands of people on native eggs and native chicken, and promoting the civil engineering of vegetable baskets.
Detailed Description
In order to further illustrate the technical effects of the present invention, the present invention will be specifically described by examples.
Example 1
The Sichuan mountain black-bone chicken feed comprises the following components in parts by weight: 40 parts of corn, 20 parts of bean pulp, 5 parts of yeast protein, 5 parts of corn protein powder, 1 part of soybean oil, 3 parts of stone powder, 2 parts of stone particles, 1 part of calcium hydrophosphate, 0.1 part of vitamin premix, 0.1 part of trace element premix, 2 parts of compound microbial inoculum, 1 part of compound enzyme, 1 part of amino acid premix, 0.2 part of salt and 0.4 part of bentonite.
The composite microbial inoculum comprises the following components in parts by weight: 4 parts of saccharomycetes, 2 parts of lactobacillus crispatus and 1 part of lactobacillus reuteri.
The mass ratio of the complex enzyme I to the complex enzyme II is 10:4; the complex enzyme I comprises, by weight, 1 part of beta-glucanase, 1 part of pectinase, 1 part of xylanase, 1 part of cellulase, 1 part of saccharifying enzyme, 1 part of neutral protease and 2 parts of protective agent, and the complex enzyme II comprises 4 parts of trypsin, 2 parts of cellulase, 1 part of sodium alginate, 0.5 part of polyanionic polysaccharide, 0.5 part of whey protein isolate and 0.5 part of soybean oil.
The protective agent comprises, by weight, 5 parts of disodium glycyrrhizate, 4 parts of vanillin, 4 parts of sodium chloride, 2 parts of stevioside, 2 parts of potassium chloride, 1 part of Chalcanthitum, 1 part of resveratrol and 1 part of myo-inositol.
The preparation method of the complex enzyme I comprises the following steps: mixing disodium glycyrrhizate, vanillin, sodium chloride, stevioside, potassium chloride and Chalcanthitum, pulverizing to 70 mesh, treating in 70deg.C steam for 3 min, rapidly cooling to 20deg.C, and standing for 1 hr to obtain mixture I; mixing resveratrol and myo-inositol, pulverizing to 30 mesh, adding 100 parts of 80% ethanol solution, maintaining ultrasonic frequency at 30deg.C at 200W, ultrasonic treating for 50 min, filtering, collecting filtrate, heating to remove ethanol, freezing at-10deg.C to-20deg.C for 8 hr, and vacuum drying for 3 hr to obtain mixture II; and uniformly mixing beta-glucanase, pectinase, xylanase, cellulase, saccharifying enzyme and neutral protease with the mixture I and the mixture II to prepare the compound enzyme I.
The preparation method of the complex enzyme II comprises the following steps: mixing trypsin and cellulase uniformly, then adding sodium alginate and polyanion polysaccharide for coating, and finally adding whey protein isolate and soybean oil for coating after mixing to prepare the compound enzyme II.
The preparation method of the Sichuan mountain black-bone chicken feed comprises the following steps: weighing the raw materials according to the proportion, uniformly mixing, adding water, uniformly stirring, granulating and air-drying in sequence to obtain the Sichuan mountain black-bone chicken feed.
Example 2
The Sichuan mountain black-bone chicken feed comprises the following components in parts by weight: 80 parts of corn, 30 parts of bean pulp, 10 parts of yeast protein, 10 parts of corn protein powder, 3 parts of soybean oil, 5 parts of stone powder, 4 parts of stone particles, 2 parts of calcium hydrophosphate, 0.2 part of vitamin premix, 0.3 part of trace element premix, 4 parts of compound microbial inoculum, 5 parts of compound enzyme, 2 parts of amino acid premix, 0.4 part of salt and 0.6 part of bentonite.
The composite microbial inoculum comprises the following components in parts by weight: 8 parts of saccharomycetes, 4 parts of lactobacillus crispatus and 3 parts of lactobacillus reuteri.
The mass ratio of the complex enzyme I to the complex enzyme II is 15:6; the complex enzyme I comprises, by weight, 3 parts of beta-glucanase, 3 parts of pectinase, 2 parts of xylanase, 2 parts of cellulase, 3 parts of saccharifying enzyme, 3 parts of neutral protease and 3 parts of protective agent, and the complex enzyme II comprises 6 parts of trypsin, 3 parts of cellulase, 2 parts of sodium alginate, 1 part of polyanionic polysaccharide, 1 part of whey protein isolate and 1 part of soybean oil.
The protective agent comprises 10 parts of disodium glycyrrhizate, 6 parts of vanillin, 6 parts of sodium chloride, 4 parts of stevioside, 4 parts of potassium chloride, 3 parts of Chalcanthitum, 2 parts of resveratrol and 2 parts of myo-inositol.
The preparation method of the complex enzyme I comprises the following steps: mixing disodium glycyrrhizate, vanillin, sodium chloride, stevioside, potassium chloride and Chalcanthitum, pulverizing to 80 mesh, treating in 80 deg.C steam for 4 min, rapidly cooling to 24 deg.C, and standing for 2 hr to obtain mixture I; mixing resveratrol and myo-inositol, pulverizing to 36 mesh, adding 200 parts of 95% ethanol solution, maintaining ultrasonic frequency at 40deg.C at 300W, ultrasonic filtering for 80 min, heating to remove ethanol, freezing at-10deg.C to-20deg.C for 9 hr, and vacuum drying for 5 hr to obtain mixture II; and uniformly mixing beta-glucanase, pectinase, xylanase, cellulase, saccharifying enzyme and neutral protease with the mixture I and the mixture II to prepare the compound enzyme I.
The preparation method of the complex enzyme II comprises the following steps: mixing trypsin and cellulase uniformly, then adding sodium alginate and polyanion polysaccharide for coating, and finally adding whey protein isolate and soybean oil for coating after mixing to prepare the compound enzyme II.
The preparation method of the Sichuan mountain black-bone chicken feed comprises the following steps: weighing the raw materials according to the proportion, uniformly mixing, adding water, uniformly stirring, granulating and air-drying in sequence to obtain the Sichuan mountain black-bone chicken feed.
Example 3
The Sichuan mountain black-bone chicken feed comprises the following components in parts by weight: 60 parts of corn, 25 parts of bean pulp, 7 parts of yeast protein, 7 parts of corn protein powder, 2 parts of soybean oil, 2 parts of stone powder, 3 parts of stone particles, 1 part of calcium hydrophosphate, 0.15 part of vitamin premix, 0.2 part of trace element premix, 3 parts of compound microbial inoculum, 3 parts of compound enzyme, 2 parts of amino acid premix, 0.3 part of salt and 0.5 part of bentonite.
The composite microbial inoculum comprises the following components in parts by weight: 6 parts of saccharomycetes, 3 parts of lactobacillus crispatus and 2 parts of lactobacillus reuteri.
The mass ratio of the complex enzyme I to the complex enzyme II is 12:5; the complex enzyme I comprises 2 parts of beta-glucanase, 2 parts of pectase, 1 part of xylanase, 2 parts of cellulase, 2 parts of saccharifying enzyme, 2 parts of neutral protease and 2 parts of protective agent, and the complex enzyme II comprises 5 parts of trypsin, 3 parts of cellulase, 1.5 parts of sodium alginate, 0.7 part of polyanionic polysaccharide, 0.7 part of whey protein isolate and 0.7 part of soybean oil.
The protective agent comprises 7 parts of disodium glycyrrhizate, 5 parts of vanillin, 5 parts of sodium chloride, 3 parts of stevioside, 3 parts of potassium chloride, 2 parts of Chalcanthitum, 2 parts of resveratrol and 1 part of myo-inositol.
The preparation method of the complex enzyme I comprises the following steps: mixing disodium glycyrrhizate, vanillin, sodium chloride, stevioside, potassium chloride and Chalcanthitum, pulverizing to 75 mesh, treating in 75 deg.C steam for 3 min, rapidly cooling to 22deg.C, and standing for 1 hr to obtain mixture I; mixing resveratrol and myo-inositol, pulverizing to 33 mesh, adding 150 parts of 90% ethanol solution, maintaining ultrasonic frequency at 35deg.C at 250W, ultrasonic treating for 65 min, filtering, collecting filtrate, heating to remove ethanol, freezing at-10deg.C to-20deg.C for 8 hr, and vacuum drying for 5 hr to obtain mixture II; and uniformly mixing beta-glucanase, pectinase, xylanase, cellulase, saccharifying enzyme and neutral protease with the mixture I and the mixture II to prepare the compound enzyme I.
The preparation method of the complex enzyme II comprises the following steps: mixing trypsin and cellulase uniformly, then adding sodium alginate and polyanion polysaccharide for coating, and finally adding whey protein isolate and soybean oil for coating after mixing to prepare the compound enzyme II.
The preparation method of the Sichuan mountain black-bone chicken feed comprises the following steps: weighing the raw materials according to the proportion, uniformly mixing, adding water, uniformly stirring, granulating and air-drying in sequence to obtain the Sichuan mountain black-bone chicken feed.
Comparative example 1
The comparative example was free of complex microbial agents, and the remaining components and preparation methods were the same as in example 1.
Comparative example 2
The comparative example was free of complex enzyme, and the rest of the components and preparation method were the same as in example 1.
Comparative example 3
The comparative example has neither complex microbial agent nor complex enzyme agent, and the rest of components and preparation method are the same as in example 1.
1 materials and methods
1.1 design of experiments
480 healthy 23-week-old Sichuan mountain black-bone chickens with similar weights were selected, and the feeds of examples 1-3 and comparative examples 1-3 were used for the test, which divided 6 treatments, 4 replicates per treatment, and 20 chickens per replicate. Pre-trial period 14d, positive trial period 120d.
1.2 test ration and feeding management
The test daily ration is prepared by referring to Chinese chicken raising Standard (NY/T33-2004) and Chinese feed ingredient and nutritive value table 2020 and combining with Sichuan mountain black-bone chicken raising technical guidelines. The experimental chickens adopt the house-outside perch type feeding instead of free-range feeding so as to meet the welfare healthy breeding of the chickens, and an egg laying box is additionally arranged, so that the collection of eggs is facilitated. Natural illumination and natural ventilation are carried out in the test period, and the food and the water are taken freely. Chicken flocks were vaccinated according to conventional immunization procedures.
1.3 measurement index and method
1.3.1 production Properties
In the test period, recording the number of eggs laid by the test chicken and the weight of the eggs per day by taking the repetition as a unit, recording the feed intake, and calculating the feed-egg ratio according to the daily egg yield and the daily feed intake.
1.3.2 slaughter Properties
After the test, 4 chickens close to the average body weight were selected from each repetition, the selected chickens were sacrificed by a neck bleed method, dehaired in hot water at 70 ℃, and after wiping off the water, the slaughter rate, the total evisceration rate, the half evisceration rate, the pectoral muscle rate and the leg muscle rate were determined.
Slaughter rate (%) = (carcass weight/pre-slaughter weight) ×100%
Full bore clearance (%) = (full bore clearance weight/pre-slaughter body weight) ×100%
Semi-clearance rate (%) = (semi-clearance weight/pre-slaughter weight) ×100%
Chest muscle rate (%) = (chest muscle weight/full-bore-free weight) ×100%
Leg muscle rate (%) = (leg muscle weight/total breech-free weight) ×100%
2 results and analysis
TABLE 1 Effect of different feeds on production Performance of Sichuan mountain bird bone chickens
Project Average egg weight (g) Laying rate (%) Daily egg yield (g) Daily feed intake (g) Ratio of feed to egg
Example 1 57.04±1.12 a 67.14±1.75 a 38.30±1.71 a 108.67±4.69 a 2.84±0.09 b
Example 2 57.53±1.16 a 69.21±1.62 a 39.82±1.96 a 110.67±5.44 a 2.78±0.05 b
Example 3 57.16±1.24 a 68.46±1.65 a 39.13±1.65 a 110.41±5.57 a 2.82±0.08 b
Comparative example 1 53.21±1.11 b 62.33±1.45 b 33.17±1.45 b 98.76±3.43 b 2.98±0.05 a
Comparative example 2 53.15±1.02 b 61.53±1.27 b 32.70±1.25 b 97.53±3.12 b 2.98±0.10 a
Comparative example 3 51.27±1.04 b 60.17±1.07 b 30.85±1.35 b 95.76±4.78 b 3.10±0.06 a
Note that: the same letter shoulder-notes of the same row of data indicate that the difference is not significant (P > 0.05); the same row of data is shoulder-annotated with different lower case letters indicating significant differences (P < 0.05), as follows.
Table 2 effect units of different feeds on slaughter performance of bird bone chickens in the hilly area of sichuan: % of (B)
Project Slaughter rate Full bore clearance rate Semi-bore clearance rate Pectoral muscle rate Leg muscle rate
Example 1 92.56±1.43 a 66.58±0.62 a 80.45±1.01 a 18.67±1.13 a 21.32±0.81 a
Example 2 93.42±1.22 a 68.42±0.53 a 81.93±0.91 a 20.95±0.88 a 23.77±0.67 a
Example 3 92.68±1.04 a 67.58±0.57 a 80.86±0.68 a 19.45±0.92 a 21.57±0.54 a
Comparative example 1 89.54±1.01 b 63.32±0.45 b 77.51±0.54 b 16.49±0.67 b 18.67±0.43 b
Comparative example 2 87.32±1.06 b 62.61±0.47 b 76.89±0.46 b 15.68±0.98 b 17.86±0.54 b
Comparative example 3 87.04±1.02 b 62.58±0.31 b 76.56±0.55 b 15.45±0.67 b 16.77±0.67 b
As is clear from tables 1 and 2, the production performance and slaughter performance of the Sichuan mountain bird bone chickens of examples 1 to 3 were more excellent than those of comparative examples 1 to 3. Proved by the invention, the average egg weight, the egg yield, the daily feed intake and the feed-egg ratio of the Sichuan mountain black-bone chicken are obviously improved by the Sichuan mountain black-bone chicken feed containing the composite microbial inoculum and the composite enzyme. Proved by the invention, the feed containing the composite microbial agent and the composite enzyme agent and the preparation method thereof have the advantages that the slaughter rate, the full-bore-removal rate, the half-bore-removal rate, the pectoral muscle rate and the leg muscle rate of the Sichuan mountain black-bone chicken are obviously improved.
Finally, it should be noted that the above embodiments are only for illustrating the technical solution of the present invention, but not for limiting the same, and although the technical solution of the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solution of the present invention may be modified or substituted equally without departing from the spirit and scope of the present invention, and all such modifications are intended to be included in the scope of the present invention.

Claims (5)

1. The Sichuan mountain black-bone chicken feed is characterized by comprising the following components in parts by weight: 40-80 parts of corn, 20-30 parts of bean pulp, 5-10 parts of yeast protein, 5-10 parts of corn protein powder, 1-3 parts of soybean oil, 3-5 parts of stone powder, 2-4 parts of stone particles, 1-2 parts of calcium hydrophosphate, 0.1-0.2 part of vitamin premix, 0.1-0.3 part of microelement premix, 2-4 parts of complex microbial inoculum, 1-5 parts of complex enzyme, 1-2 parts of amino acid premix, 0.2-0.4 part of salt and 0.4-0.6 part of bentonite;
wherein, the composite microbial inoculum comprises the following components in parts by weight: 4-8 parts of saccharomycetes, 2-4 parts of lactobacillus crispatus and 1-3 parts of lactobacillus reuteri;
the complex enzyme agent comprises complex enzyme I and complex enzyme II; the mass ratio of the complex enzyme I to the complex enzyme II is 10-15:4-6; the complex enzyme I comprises, by weight, 1-3 parts of beta-glucanase, 1-3 parts of pectase, 1-2 parts of xylanase, 1-2 parts of cellulase, 1-3 parts of saccharifying enzyme, 1-3 parts of neutral protease and 2-3 parts of protective agent, and the complex enzyme II comprises 4-6 parts of trypsin, 2-3 parts of cellulase, 1-2 parts of sodium alginate, 0.5-1 part of polyanionic polysaccharide, 0.5-1 part of whey protein isolate and 0.5-1 part of soybean oil.
2. The feed for the black-bone chickens in the Sichuan mountain areas, according to claim 1, wherein the protective agent comprises, by weight, 5-10 parts of disodium glycyrrhizate, 4-6 parts of vanillin, 4-6 parts of sodium chloride, 2-4 parts of stevioside, 2-4 parts of potassium chloride, 1-3 parts of Chalcanthitum, 1-2 parts of resveratrol and 1-2 parts of myo-inositol.
3. The preparation method of the Sichuan mountain black-bone chicken feed according to claim 1, which is characterized by comprising the following steps: weighing the raw materials according to the proportion, uniformly mixing, adding water, uniformly stirring, granulating and air-drying in sequence to obtain the Sichuan mountain black-bone chicken feed.
4. A method of preparing a complex enzyme according to claim 3, comprising the steps of: mixing disodium glycyrrhizate, vanillin, sodium chloride, stevioside, potassium chloride and Chalcanthitum, pulverizing to 70-80 mesh, treating in 70-80deg.C steam for 3-4 min, rapidly cooling to 20-24deg.C, and standing for 1-2 hr to obtain mixture I; mixing resveratrol and myo-inositol, pulverizing to 30-36 mesh, adding 100-200 parts of 80-95% ethanol solution, maintaining ultrasonic frequency at 30-40deg.C at 200-300W, filtering after 50-80 min, collecting filtrate, heating to remove ethanol, freezing at-10deg.C to-20deg.C for 8-9 hr, and vacuum drying for 3-5 hr to obtain mixture II; and uniformly mixing beta-glucanase, pectinase, xylanase, cellulase, saccharifying enzyme and neutral protease with the mixture I and the mixture II to prepare the compound enzyme I.
5. The method of preparing the complex enzyme II according to claim 4, wherein the method of preparing the complex enzyme II comprises the steps of: mixing trypsin and cellulase uniformly, then adding sodium alginate and polyanion polysaccharide for coating, and finally adding whey protein isolate and soybean oil for coating after mixing to prepare the compound enzyme II.
CN202110308386.2A 2021-03-23 2021-03-23 Sichuan mountain black-bone chicken feed and preparation method thereof Active CN112970965B (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202110308386.2A CN112970965B (en) 2021-03-23 2021-03-23 Sichuan mountain black-bone chicken feed and preparation method thereof
AU2021103023A AU2021103023A4 (en) 2021-03-23 2021-06-02 Sichuan mountain black-bone chicken feed and method for preparing the same

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202110308386.2A CN112970965B (en) 2021-03-23 2021-03-23 Sichuan mountain black-bone chicken feed and preparation method thereof

Publications (2)

Publication Number Publication Date
CN112970965A CN112970965A (en) 2021-06-18
CN112970965B true CN112970965B (en) 2023-07-14

Family

ID=76333201

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202110308386.2A Active CN112970965B (en) 2021-03-23 2021-03-23 Sichuan mountain black-bone chicken feed and preparation method thereof

Country Status (2)

Country Link
CN (1) CN112970965B (en)
AU (1) AU2021103023A4 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114601072A (en) * 2022-03-25 2022-06-10 北京九州大地生物技术集团股份有限公司 Feed composition for reducing heat stress of poultry and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104171538A (en) * 2014-07-07 2014-12-03 辽宁禾丰牧业股份有限公司 Saving-type daily ration for laying hens during egg laying peak and preparation method thereof
CN105192379A (en) * 2015-10-08 2015-12-30 南涧县兴农发展有限公司 Ecological-type high-quality concentrated feed for Wuliang mountain silky fowl
CN109043210A (en) * 2018-07-23 2018-12-21 广西万寿谷投资集团股份有限公司 A kind of free-ranging chicken chicken feed and preparation method thereof
CN109329583A (en) * 2018-12-12 2019-02-15 海城市盛利饲料有限公司 A kind of broiler fodder

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102940135B (en) * 2012-09-25 2013-09-18 东莞市双胞胎饲料有限公司 Efficient pig feed composite enzyme preparation added with enzyme protective agent
CN103892134B (en) * 2014-04-15 2015-09-16 湖南农业大学 A kind of egg feedstuff complex enzyme formulation and production method thereof and application
CN105524905A (en) * 2014-09-29 2016-04-27 湖南新鸿鹰生物工程有限公司 Acid protease containing compound enzyme of soy sauce and preparation method of acid protease containing compound enzyme of soy sauce
CN104957427A (en) * 2015-07-14 2015-10-07 四川圣迪乐村生态食品股份有限公司 Layer feed for keeping layer high yield and preventing fatty liver
CN106666204A (en) * 2017-01-05 2017-05-17 辽宁禾丰牧业股份有限公司 Feed used for 72-100-week-old laying hens and capable of improving immune function and preparation method of feed
CN110558448A (en) * 2019-10-21 2019-12-13 宁夏晓鸣农牧股份有限公司 Feed for chickens in egg producing period and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104171538A (en) * 2014-07-07 2014-12-03 辽宁禾丰牧业股份有限公司 Saving-type daily ration for laying hens during egg laying peak and preparation method thereof
CN105192379A (en) * 2015-10-08 2015-12-30 南涧县兴农发展有限公司 Ecological-type high-quality concentrated feed for Wuliang mountain silky fowl
CN109043210A (en) * 2018-07-23 2018-12-21 广西万寿谷投资集团股份有限公司 A kind of free-ranging chicken chicken feed and preparation method thereof
CN109329583A (en) * 2018-12-12 2019-02-15 海城市盛利饲料有限公司 A kind of broiler fodder

Also Published As

Publication number Publication date
AU2021103023A4 (en) 2021-07-15
CN112970965A (en) 2021-06-18

Similar Documents

Publication Publication Date Title
CN105595008B (en) Laying period compound feed for improving healthy chick rate of egg-laying hens and preparation method thereof
CN104824462B (en) A kind of largemouth bass fingerling mixed feed
CN108998391B (en) Composite microbial fermentation agent, biological fermentation feed prepared by using fermentation agent and preparation method of biological fermentation feed
CN107494911B (en) Method for breeding animals by using antibiotic-free feed containing astragalus stem, leaf and flower
CN105638564B (en) A kind of high-yield egg chicken medelling method for breeding extending laying period
CN103960547A (en) Low-protein weanling piglet feed and preparation method thereof
CN105053566B (en) A kind of bicycle beam wood seed Pepsin feed addictive and preparation method thereof
CN103704486B (en) Poultry enzyme including neutral protease and preparation method thereof
CN107912360B (en) White feather broiler feeding mode
CN1919044A (en) Animal feedstuff additive and preparation method thereof
CN102726596A (en) Feather protein peptide feed additive and application thereof
CN106173455A (en) A kind of multi-functional chicken feed and preparation method
CN104256151A (en) Feed for yellow meal worms and preparation method thereof
CN112970965B (en) Sichuan mountain black-bone chicken feed and preparation method thereof
CN111011654A (en) Compound feed for micropterus salmoides fry
CN112538436B (en) Preparation method of acremonium terricola culture
CN104489407B (en) Premixed feed for promoting development of ovaries of laying hens
CN104905030B (en) Ussuriensis larva and juvenile special compound feed and preparation method thereof
CN106614306A (en) Native chicken raising method
CN107348222A (en) A kind of preparation method of livestock culture feed
CN107041456B (en) Fermented bean dreg feed additive and preparation method and application thereof
CN106811429B (en) Bacillus subtilis strain, application of feed additive thereof and feed
CN115024418A (en) Mulberry leaf feed for pigs, preparation method and application thereof
CN107712301A (en) Improve the feed of meat chicken production performance
CN104187059B (en) Earthworm meal growth promoter for pork pigs and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant