CN112970965A - Sichuan mountain black-bone chicken feed and preparation method thereof - Google Patents
Sichuan mountain black-bone chicken feed and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a Sichuan mountain black-bone chicken feed and a preparation method thereof, belonging to the technical field of feeds, wherein the Sichuan mountain black-bone chicken feed comprises the following components in parts by weight: 40-80 parts of corn, 20-30 parts of soybean meal, 5-10 parts of yeast protein, 5-10 parts of corn protein powder, 1-3 parts of soybean oil, 3-5 parts of stone powder, 2-4 parts of stone particles, 1-2 parts of calcium hydrophosphate, 0.1-0.2 part of vitamin premix, 0.1-0.3 part of trace element premix, 2-4 parts of complex microbial inoculum, 1-5 parts of complex enzyme agent, 1-2 parts of amino acid premix, 0.2-0.4 part of salt and 0.4-0.6 part of bentonite; according to the formula, the compound microbial inoculum and the compound enzyme agent are reasonably mixed, so that the nutrient absorption efficiency is improved, the immunity of an organism is enhanced, the disease occurrence proportion is reduced, and the use of medicines at a breeding end is avoided, so that the safe and reassuring production of black-bone eggs and black-bone chicken with excellent quality is ensured, the consumption requirements of people on the native eggs and the native chicken are met, and the vegetable basket life engineering is promoted.
Description
Technical Field
The invention belongs to the technical field of feeds, and particularly relates to a Sichuan mountain black-bone chicken feed and a preparation method thereof.
Background
The Sichuan mountain silkie belongs to a meat and egg dual-purpose local chicken variety, is formed by blocking and breeding Sichuan local chicken species silkie, is mainly produced in southern Sichuan and southwest mountainous areas, and has wide distribution and large quantity. The chicken has the characteristics of wide adaptability, strong disease resistance and stable genetic performance. According to detection, the feed only contains 11% -12% of crude protein, and if the supplementary feeding of the chickens is insufficient, the nutrition imbalance is easy to cause, the daily egg yield is low, the meat production performance is poor, and meanwhile, the difficulty in collecting eggs in the feeding mode is high. It has been found that a layer ration containing 11% to 12% crude protein is theoretically sufficient, but in order to achieve better productivity, levels of no less than 14% crude protein are actually required.
Moreover, due to the shortage of high-quality protein feed raw materials in China, the use of a large amount of unconventional protein raw materials in the feed for the Sichuan mountain silky fowl causes great negative effects on the breeding benefits, and meanwhile, the activities of the microbial inoculum and the enzyme agent in the existing feed are reduced along with the prolonging of time, so that the absorption of crude protein of the feed is unstable, and the production performance and slaughtering performance of the Sichuan mountain silky fowl are greatly influenced.
At present, researches on feed for Sichuan mountain black-bone chickens are quite lacking, and in order to improve the quality of meat and egg products of Sichuan mountain black-bone chickens and promote large-scale breeding production of high-quality Sichuan mountain black-bone chicken varieties, related researches are very necessary to be carried out on the preparation of the feed for Sichuan mountain black-bone chickens, particularly on the application of microbial agents and enzyme agents in the feed.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide Sichuan mountain silky fowl feed and a preparation method thereof.
In order to achieve the purpose, the invention adopts the technical scheme that:
a Sichuan mountain black-bone chicken feed comprises the following components in parts by weight: 40-80 parts of corn, 20-30 parts of soybean meal, 5-10 parts of yeast protein, 5-10 parts of corn protein powder, 1-3 parts of soybean oil, 3-5 parts of stone powder, 2-4 parts of stone particles, 1-2 parts of calcium hydrophosphate, 0.1-0.2 part of vitamin premix, 0.1-0.3 part of trace element premix, 2-4 parts of complex microbial inoculum, 1-5 parts of compound enzyme agent, 1-2 parts of amino acid premix, 0.2-0.4 part of salt and 0.4-0.6 part of bentonite.
Further, the complex microbial inoculum comprises any four of yeast, lactobacillus casei, lactobacillus crispatus and lactobacillus reuteri.
Further, the complex microbial inoculum comprises yeast, lactobacillus crispatus and lactobacillus reuteri.
Further, the compound microbial inoculum comprises the following components in parts by weight: 4-8 parts of yeast, 2-4 parts of lactobacillus crispatus and 1-3 parts of lactobacillus reuteri.
Further, the compound enzyme agent comprises a compound enzyme I and a compound enzyme II; the complex enzyme I comprises beta-glucanase, pectinase, xylanase, cellulase, glucoamylase, neutral protease and a protective agent, and the complex enzyme II comprises trypsin, cellulase, sodium alginate, polyanionic polysaccharide, whey protein isolate and soybean oil.
Further, the mass ratio of the compound enzyme I to the compound enzyme II is 10-15: 4-6; the compound enzyme I comprises 1-3 parts of beta-glucanase, 1-3 parts of pectinase, 1-2 parts of xylanase, 1-2 parts of cellulase, 1-3 parts of glucoamylase, 1-3 parts of neutral protease and 2-3 parts of a protective agent by weight, and the compound enzyme II comprises 4-6 parts of trypsin, 2-3 parts of cellulase, 1-2 parts of sodium alginate, 0.5-1 part of polyanionic polysaccharide, 0.5-1 part of whey protein isolate and 0.5-1 part of soybean oil by weight.
Further, the protective agent comprises, by weight, 5-10 parts of disodium glycyrrhizinate, 4-6 parts of vanillin, 4-6 parts of sodium chloride, 2-4 parts of stevioside, 2-4 parts of potassium chloride, 1-3 parts of chalcanthite, 1-2 parts of resveratrol and 1-2 parts of meso-inositol.
The preparation method of the Sichuan mountain black-bone chicken feed comprises the following steps: weighing the raw materials according to the proportion, uniformly mixing, adding water, uniformly stirring, and sequentially granulating and air-drying to obtain the Sichuan mountain black-bone chicken feed.
Further, the preparation method of the complex enzyme I comprises the following steps: mixing disodium glycyrrhizinate, vanillin, sodium chloride, stevioside, potassium chloride and Chalcanthitum, pulverizing to 70-80 mesh, treating in 70-80 deg.C steam for 3-4 min, rapidly cooling to 20-24 deg.C, and standing for 1-2 hr to obtain mixture I; mixing resveratrol and meso-inositol, crushing to 30-36 meshes, adding 100-200 parts of ethanol solution with the mass fraction of 80-95%, keeping the ultrasonic frequency at 300W at 30-40 ℃, filtering after ultrasonic treatment for 50-80 minutes, taking the filtrate, heating to remove ethanol, freezing at-10 ℃ to-20 ℃ for 8-9 hours, and then vacuum drying for 3-5 hours to obtain a mixture II; and uniformly mixing the beta-glucanase, the pectinase, the xylanase, the cellulase, the glucoamylase and the neutral protease with the mixture I and the mixture II to prepare the complex enzyme I.
Further, the preparation method of the complex enzyme II comprises the following steps: mixing trypsin and cellulase uniformly, adding sodium alginate and polyanionic polysaccharide for coating, and finally adding the mixed whey protein isolate and soybean oil for coating to prepare the complex enzyme II.
Furthermore, the vitamin premix, the trace element premix and the amino acid premix can be commercially available premixes.
The invention inspects the daily ration prepared by the microbial inoculum and the enzyme agent, researches the influence of the daily ration on the production performance and slaughtering performance of Sichuan mountain black-bone chickens, and provides a test basis for fully exerting the production potential of the Sichuan mountain black-bone chickens and improving the production performance of the Sichuan mountain black-bone chickens.
The invention has the beneficial effects that:
according to the invention, by feeding the compound feed containing multiple complex microbial agents and complex enzyme agents to the Sichuan mountain silky fowl, the use of unconventional feed resources is reduced, the digestibility of protein is improved, the raw materials of the protein feed are saved, and the breeding benefit is improved; the feed prepared by the formula is used for feeding Sichuan mountain land black-bone chickens, so that the laying rate and the meat yield of the Sichuan mountain land black-bone chickens can be remarkably improved; according to the formula, the compound microbial inoculum and the compound enzyme agent are reasonably mixed, so that the nutrient absorption efficiency is improved, the immunity of an organism is enhanced, the disease occurrence proportion is reduced, and the use of medicines at a breeding end is avoided, so that the safe and reassuring production of black-bone eggs and black-bone chicken with excellent quality is ensured, the consumption requirements of people on the native eggs and the native chicken are met, and the vegetable basket life engineering is promoted.
Detailed Description
In order to further illustrate the technical effects of the present invention, the present invention is specifically described below by way of examples.
Example 1
The feed for Sichuan mountain black-bone chickens comprises the following components in parts by weight: 40 parts of corn, 20 parts of soybean meal, 5 parts of yeast protein, 5 parts of corn protein powder, 1 part of soybean oil, 3 parts of stone powder, 2 parts of stone particles, 1 part of calcium hydrophosphate, 0.1 part of vitamin premix, 0.1 part of microelement premix, 2 parts of complex microbial inoculum, 1 part of complex enzyme agent, 1 part of amino acid premix, 0.2 part of salt and 0.4 part of bentonite.
The composite microbial inoculum comprises the following components in parts by weight: 4 parts of yeast, 2 parts of lactobacillus crispatus and 1 part of lactobacillus reuteri.
The mass ratio of the compound enzyme I to the compound enzyme II is 10: 4; the compound enzyme I comprises 1 part of beta-glucanase, 1 part of pectinase, 1 part of xylanase, 1 part of cellulase, 1 part of glucoamylase, 1 part of neutral protease and 2 parts of protective agent, and the compound enzyme II comprises 4 parts of trypsin, 2 parts of cellulase, 1 part of sodium alginate, 0.5 part of polyanionic polysaccharide, 0.5 part of whey protein isolate and 0.5 part of soybean oil.
The protective agent comprises, by weight, 5 parts of disodium glycyrrhizinate, 4 parts of vanillin, 4 parts of sodium chloride, 2 parts of stevioside, 2 parts of potassium chloride, 1 part of chalcanthite, 1 part of resveratrol and 1 part of meso-inositol.
The preparation method of the complex enzyme I comprises the following steps: mixing disodium glycyrrhizinate, vanillin, sodium chloride, stevioside, potassium chloride and Chalcanthitum, pulverizing to 70 mesh, treating in 70 deg.C steam for 3 min, rapidly cooling to 20 deg.C, and standing for 1 hr to obtain mixture I; mixing resveratrol and meso-inositol, crushing to 30 meshes, adding 100 parts of ethanol solution with the mass fraction of 80%, keeping the ultrasonic frequency at 30 ℃ at 200W, performing ultrasonic treatment for 50 minutes, filtering, taking filtrate, heating to remove ethanol, freezing at-10 ℃ to-20 ℃ for 8 hours, and performing vacuum drying for 3 hours to obtain a mixture II; and uniformly mixing the beta-glucanase, the pectinase, the xylanase, the cellulase, the glucoamylase and the neutral protease with the mixture I and the mixture II to prepare the complex enzyme I.
The preparation method of the compound enzyme II comprises the following steps: mixing trypsin and cellulase uniformly, adding sodium alginate and polyanionic polysaccharide for coating, and finally adding the mixed whey protein isolate and soybean oil for coating to prepare the complex enzyme II.
The preparation method of the Sichuan mountain black-bone chicken feed comprises the following steps: weighing the raw materials according to the proportion, uniformly mixing, adding water, uniformly stirring, and sequentially granulating and air-drying to obtain the Sichuan mountain black-bone chicken feed.
Example 2
The feed for Sichuan mountain black-bone chickens comprises the following components in parts by weight: 80 parts of corn, 30 parts of soybean meal, 10 parts of yeast protein, 10 parts of corn protein powder, 3 parts of soybean oil, 5 parts of stone powder, 4 parts of stone particles, 2 parts of calcium hydrophosphate, 0.2 part of vitamin premix, 0.3 part of microelement premix, 4 parts of composite microbial inoculum, 5 parts of composite enzyme agent, 2 parts of amino acid premix, 0.4 part of salt and 0.6 part of bentonite.
The composite microbial inoculum comprises the following components in parts by weight: 8 parts of yeast, 4 parts of lactobacillus crispatus and 3 parts of lactobacillus reuteri.
The mass ratio of the compound enzyme I to the compound enzyme II is 15: 6; the compound enzyme I comprises 3 parts of beta-glucanase, 3 parts of pectinase, 2 parts of xylanase, 2 parts of cellulase, 3 parts of glucoamylase, 3 parts of neutral protease and 3 parts of a protective agent, and the compound enzyme II comprises 6 parts of trypsin, 3 parts of cellulase, 2 parts of sodium alginate, 1 part of polyanionic polysaccharide, 1 part of whey protein isolate and 1 part of soybean oil.
The protective agent comprises, by weight, 10 parts of disodium glycyrrhizinate, 6 parts of vanillin, 6 parts of sodium chloride, 4 parts of stevioside, 4 parts of potassium chloride, 3 parts of chalcanthite, 2 parts of resveratrol and 2 parts of meso-inositol.
The preparation method of the complex enzyme I comprises the following steps: mixing disodium glycyrrhizinate, vanillin, sodium chloride, stevioside, potassium chloride and Chalcanthitum, pulverizing to 80 mesh, treating in 80 deg.C steam for 4 min, rapidly cooling to 24 deg.C, and standing for 2 hr to obtain mixture I; mixing resveratrol and meso-inositol, crushing to 36 meshes, adding 200 parts of ethanol solution with the mass fraction of 95%, keeping the ultrasonic frequency at 300W at 40 ℃, filtering after ultrasonic treatment for 80 minutes, taking the filtrate, heating to remove ethanol, freezing at-10 ℃ to-20 ℃ for 9 hours, and then drying in vacuum for 5 hours to obtain a mixture II; and uniformly mixing the beta-glucanase, the pectinase, the xylanase, the cellulase, the glucoamylase and the neutral protease with the mixture I and the mixture II to prepare the complex enzyme I.
The preparation method of the compound enzyme II comprises the following steps: mixing trypsin and cellulase uniformly, adding sodium alginate and polyanionic polysaccharide for coating, and finally adding the mixed whey protein isolate and soybean oil for coating to prepare the complex enzyme II.
The preparation method of the Sichuan mountain black-bone chicken feed comprises the following steps: weighing the raw materials according to the proportion, uniformly mixing, adding water, uniformly stirring, and sequentially granulating and air-drying to obtain the Sichuan mountain black-bone chicken feed.
Example 3
The Sichuan mountain black-bone chicken feed comprises the following components in parts by weight: 60 parts of corn, 25 parts of soybean meal, 7 parts of yeast protein, 7 parts of corn protein powder, 2 parts of soybean oil, 2 parts of stone powder, 3 parts of stone particles, 1 part of calcium hydrophosphate, 0.15 part of vitamin premix, 0.2 part of microelement premix, 3 parts of composite microbial inoculum, 3 parts of composite enzyme agent, 2 parts of amino acid premix, 0.3 part of salt and 0.5 part of bentonite.
The composite microbial inoculum comprises the following components in parts by weight: 6 parts of yeast, 3 parts of lactobacillus crispatus and 2 parts of lactobacillus reuteri.
The mass ratio of the compound enzyme I to the compound enzyme II is 12: 5; the compound enzyme I comprises 2 parts of beta-glucanase, 2 parts of pectinase, 1 part of xylanase, 2 parts of cellulase, 2 parts of glucoamylase, 2 parts of neutral protease and 2 parts of a protective agent by weight, and the compound enzyme II comprises 5 parts of trypsin, 3 parts of cellulase, 1.5 parts of sodium alginate, 0.7 part of polyanionic polysaccharide, 0.7 part of whey protein isolate and 0.7 part of soybean oil by weight.
The protective agent comprises 7 parts of disodium glycyrrhizinate, 5 parts of vanillin, 5 parts of sodium chloride, 3 parts of stevioside, 3 parts of potassium chloride, 2 parts of chalcanthite, 2 parts of resveratrol and 1 part of meso-inositol in parts by weight.
The preparation method of the complex enzyme I comprises the following steps: mixing disodium glycyrrhizinate, vanillin, sodium chloride, stevioside, potassium chloride and Chalcanthitum, pulverizing to 75 meshes, treating in 75 deg.C steam for 3 min, rapidly cooling to 22 deg.C, and standing for 1 hr to obtain mixture I; mixing resveratrol and meso-inositol, crushing to 33 meshes, adding 150 parts of ethanol solution with the mass fraction of 90%, keeping the ultrasonic frequency at 250W at 35 ℃, filtering after ultrasonic treatment for 65 minutes, taking the filtrate, heating to remove ethanol, freezing at-10 ℃ to-20 ℃ for 8 hours, and then drying in vacuum for 5 hours to obtain a mixture II; and uniformly mixing the beta-glucanase, the pectinase, the xylanase, the cellulase, the glucoamylase and the neutral protease with the mixture I and the mixture II to prepare the complex enzyme I.
The preparation method of the compound enzyme II comprises the following steps: mixing trypsin and cellulase uniformly, adding sodium alginate and polyanionic polysaccharide for coating, and finally adding the mixed whey protein isolate and soybean oil for coating to prepare the complex enzyme II.
The preparation method of the Sichuan mountain black-bone chicken feed comprises the following steps: weighing the raw materials according to the proportion, uniformly mixing, adding water, uniformly stirring, and sequentially granulating and air-drying to obtain the Sichuan mountain black-bone chicken feed.
Comparative example 1
The comparative example has no complex microbial inoculum, and the other components and the preparation method are the same as those of the example 1.
Comparative example 2
The comparative example has no complex enzyme, and the rest components and preparation method are the same as those in example 1.
Comparative example 3
The comparative example has neither complex microbial inoculum nor complex enzyme, and the rest components and the preparation method are the same as those in the example 1.
1 materials and methods
1.1 design of the experiment
480 healthy Sichuan mountain black-bone chickens with similar body weight and 23 weeks old were selected and tested by using the feeds of examples 1-3 and comparative examples 1-3, and the test was divided into 6 treatments, each treatment was repeated for 4 times, and each treatment was repeated for 20 chickens. Pre-run period 14d, positive run period 120 d.
1.2 Experimental daily ration and feeding management
The test daily ration is prepared by referring to the chicken feeding standard (NY/T33-2004) and the Chinese feed ingredient and nutrition value table (2020) in China and combining with the technical guidance of Sichuan mountain land black-bone chicken breeding. Experimental chicken is raised by adopting a henhouse external resting frame type instead of free-ranging to meet the welfare and healthy breeding of the chicken, and an egg laying box is additionally arranged to facilitate egg collection. In the test period, the test bed is naturally illuminated and ventilated, and people can freely eat and drink water. The chicken flocks are immunized according to the conventional immunization program.
1.3 measurement index and method
1.3.1 Performance
In the test period, the number of eggs laid by the test chicken and the egg weight are recorded by taking repetition as a unit, the feed intake is recorded, and the feed-egg ratio is calculated according to the daily egg yield and the daily feed intake.
1.3.2 slaughter Performance
After the test was completed, 4 chickens close to the average body weight were selected from each repetition, the selected chickens were sacrificed by cervical exsanguination, dehaired in hot water at 70 ℃, and after wiping off water, the slaughter rate, the full-bore rate, the half-bore rate, the pectoral muscle rate, and the leg muscle rate were measured.
Slaughter rate (%) (carcass weight/pre-carcass weight) × 100%
Full bore ratio (%) (full bore weight/pre-slaughter body weight) × 100%
Half bore ratio (%) (half bore weight/pre-slaughter body weight) × 100%
Breast muscle ratio (%) - (breast muscle weight/total dry weight) × 100%
The leg muscle ratio (%) (leg muscle weight/total bore weight) × 100%
2 results and analysis
TABLE 1 influence of different feeds on the performance of Sichuan mountain avians
| Item | Average egg weight (g) | Laying rate (%) | Daily egg production (g) | Daily food intake (g) | Material to egg ratio |
| Example 1 | 57.04±1.12a | 67.14±1.75a | 38.30±1.71a | 108.67±4.69a | 2.84±0.09b |
| Example 2 | 57.53±1.16a | 69.21±1.62a | 39.82±1.96a | 110.67±5.44a | 2.78±0.05b |
| Example 3 | 57.16±1.24a | 68.46±1.65a | 39.13±1.65a | 110.41±5.57a | 2.82±0.08b |
| Comparative example 1 | 53.21±1.11b | 62.33±1.45b | 33.17±1.45b | 98.76±3.43b | 2.98±0.05a |
| Comparative example 2 | 53.15±1.02b | 61.53±1.27b | 32.70±1.25b | 97.53±3.12b | 2.98±0.10a |
| Comparative example 3 | 51.27±1.04b | 60.17±1.07b | 30.85±1.35b | 95.76±4.78b | 3.10±0.06a |
Note: the same data is annotated with the same letter to indicate that the difference is not significant (P > 0.05); the same row data are annotated with different lower case letters to indicate significant differences (P <0.05), as shown in the table below.
Table 2 influence units of different feeds on slaughtering performance of chicken from mountainous region of Sichuan: is based on
| Item | Slaughter rate | Full bore clearance | Semi-bore clearance rate | Rate of pectoral muscles | Rate of leg muscles |
| Example 1 | 92.56±1.43a | 66.58±0.62a | 80.45±1.01a | 18.67±1.13a | 21.32±0.81a |
| Example 2 | 93.42±1.22a | 68.42±0.53a | 81.93±0.91a | 20.95±0.88a | 23.77±0.67a |
| Example 3 | 92.68±1.04a | 67.58±0.57a | 80.86±0.68a | 19.45±0.92a | 21.57±0.54a |
| Comparative example 1 | 89.54±1.01b | 63.32±0.45b | 77.51±0.54b | 16.49±0.67b | 18.67±0.43b |
| Comparative example 2 | 87.32±1.06b | 62.61±0.47b | 76.89±0.46b | 15.68±0.98b | 17.86±0.54b |
| Comparative example 3 | 87.04±1.02b | 62.58±0.31b | 76.56±0.55b | 15.45±0.67b | 16.77±0.67b |
As is clear from tables 1 and 2, the production performance and slaughter performance of the Sichuan mountain avian-bone chickens of examples 1 to 3 were more excellent than those of comparative examples 1 to 3. The invention proves that the Sichuan mountain land black-bone chicken feed containing the compound microbial inoculum and the compound enzyme and the preparation method thereof have obvious improvement on the average egg weight, the laying rate, the daily egg laying amount, the daily feed intake and the feed-egg ratio of Sichuan mountain black-bone chicken. The invention proves that the Sichuan mountain land black-bone chicken feed containing the compound microbial inoculum and the compound enzyme and the preparation method thereof obviously improve the dressing percentage, the full-bore-cleaning rate, the half-bore-cleaning rate, the breast muscle rate and the leg muscle rate of Sichuan mountain black-bone chickens.
Finally, it should be noted that the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the technical solutions of the present invention are described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions can be made on the technical solutions of the present invention without departing from the spirit and scope of the present invention, which should be covered by the protection scope of the present invention.
Claims (10)
1. The feed for Sichuan mountain black-bone chickens is characterized by comprising the following components in parts by weight: 40-80 parts of corn, 20-30 parts of soybean meal, 5-10 parts of yeast protein, 5-10 parts of corn protein powder, 1-3 parts of soybean oil, 3-5 parts of stone powder, 2-4 parts of stone particles, 1-2 parts of calcium hydrophosphate, 0.1-0.2 part of vitamin premix, 0.1-0.3 part of trace element premix, 2-4 parts of complex microbial inoculum, 1-5 parts of compound enzyme agent, 1-2 parts of amino acid premix, 0.2-0.4 part of salt and 0.4-0.6 part of bentonite.
2. The Sichuan mountain land black-bone chicken feed as claimed in claim 1, wherein the complex microbial inoculum comprises any four of yeast, lactobacillus casei, lactobacillus crispatus and lactobacillus reuteri.
3. The Sichuan mountain land black-bone chicken feed as claimed in claim 2, wherein the complex microbial inoculum comprises yeast, lactobacillus crispatus and lactobacillus reuteri.
4. The Sichuan mountain black-bone chicken feed as claimed in claim 3, wherein the complex microbial inoculum comprises, in parts by weight: 4-8 parts of yeast, 2-4 parts of lactobacillus crispatus and 1-3 parts of lactobacillus reuteri.
5. The Sichuan mountain silky fowl feed according to any one of claims 1 to 4, wherein the compound enzyme agent comprises compound enzyme I and compound enzyme II; the complex enzyme I comprises beta-glucanase, pectinase, xylanase, cellulase, glucoamylase, neutral protease and a protective agent, and the complex enzyme II comprises trypsin, cellulase, sodium alginate, polyanionic polysaccharide, whey protein isolate and soybean oil.
6. The Sichuan mountain land black-bone chicken feed as claimed in claim 5, wherein the mass ratio of the complex enzyme I to the complex enzyme II is 10-15: 4-6; the compound enzyme I comprises 1-3 parts of beta-glucanase, 1-3 parts of pectinase, 1-2 parts of xylanase, 1-2 parts of cellulase, 1-3 parts of glucoamylase, 1-3 parts of neutral protease and 2-3 parts of a protective agent by weight, and the compound enzyme II comprises 4-6 parts of trypsin, 2-3 parts of cellulase, 1-2 parts of sodium alginate, 0.5-1 part of polyanionic polysaccharide, 0.5-1 part of whey protein isolate and 0.5-1 part of soybean oil by weight.
7. The Sichuan mountain silky fowl feed as claimed in claim 6, wherein the protective agent comprises, by weight, 5-10 parts of disodium glycyrrhizinate, 4-6 parts of vanillin, 4-6 parts of sodium chloride, 2-4 parts of stevioside, 2-4 parts of potassium chloride, 1-3 parts of chalcanthite, 1-2 parts of resveratrol and 1-2 parts of myo-inositol.
8. The preparation method of the Sichuan mountain silky fowl feed according to claim 7, which comprises the following steps: weighing the raw materials according to the proportion, uniformly mixing, adding water, uniformly stirring, and sequentially granulating and air-drying to obtain the Sichuan mountain black-bone chicken feed.
9. The preparation method of claim 8, wherein the preparation method of the complex enzyme I comprises the following steps: mixing disodium glycyrrhizinate, vanillin, sodium chloride, stevioside, potassium chloride and Chalcanthitum, pulverizing to 70-80 mesh, treating in 70-80 deg.C steam for 3-4 min, rapidly cooling to 20-24 deg.C, standing for 1-2 hr to obtain mixture I; mixing resveratrol and meso-inositol, crushing to 30-36 meshes, adding 100-200 parts of ethanol solution with the mass fraction of 80-95%, keeping the ultrasonic frequency at 300W at 30-40 ℃, filtering after ultrasonic treatment for 50-80 minutes, taking the filtrate, heating to remove ethanol, freezing at-10 ℃ to-20 ℃ for 8-9 hours, and then vacuum drying for 3-5 hours to obtain a mixture II; and uniformly mixing the beta-glucanase, the pectinase, the xylanase, the cellulase, the glucoamylase and the neutral protease with the mixture I and the mixture II to prepare the complex enzyme I.
10. The preparation method of claim 9, wherein the preparation method of the complex enzyme II comprises the following steps: mixing trypsin and cellulase uniformly, adding sodium alginate and polyanionic polysaccharide for coating, and finally adding the mixed whey protein isolate and soybean oil for coating to prepare the complex enzyme II.
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| CN114601072A (en) * | 2022-03-25 | 2022-06-10 | 北京九州大地生物技术集团股份有限公司 | Feed composition for reducing heat stress of poultry and preparation method thereof |
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| AU2021103023A4 (en) | 2021-07-15 |
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