CN109329583A - A kind of broiler fodder - Google Patents
A kind of broiler fodder Download PDFInfo
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- CN109329583A CN109329583A CN201811515492.2A CN201811515492A CN109329583A CN 109329583 A CN109329583 A CN 109329583A CN 201811515492 A CN201811515492 A CN 201811515492A CN 109329583 A CN109329583 A CN 109329583A
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- additive
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- acid bacteria
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention discloses a kind of broiler fodder, and formula is by weight as follows: corn 590-690, dregs of beans 243-285, wheat 70-80, cotton dregs 9-24, calcium monohydrogen phosphate 4.9-5.2, calcium powder 9-11, lysine 3.2-3.8, choline chloride 0.7-1.2, DL- methionine 1.4-1.5, phytase 0.12-0.15, threonine 0.07-0.09, sodium chloride 2.5-3, bio-additive 2.8-4.7.The invention has the advantages that bio-additive is lactic acid bacteria, bacillus subtilis, feed manna oligosacchride, complex enzyme formulation, drug resistance will not be led to the problem of, it is green non-pollution additive, the use of bio-additive can overcome adverse effect of the antibiotic to other beneficial microbe colonies in alimentary canal, avoid toxic side effect, drug resistance and drug resistance that antibiotic is used for a long time, reduce residual of the drug in animal products, the quality of animal products is improved, threat of the medicament residue to human health and life security is reduced.
Description
Technical field
The present invention relates to field of feed, especially a breeding hen feed.
Background technique
In the modernization raising of broiler chicken, feed is related to the growth cycle of broiler chicken, nutritional ingredient etc..In order to guarantee broiler chicken
Normal production, prevents disease, has many feeds to use antibiotic, seldom suffers from the growth course of broiler chicken although ensure that in this way
Disease, but antibiotic is absorbed by people indirectly, causes injury to human body.
Summary of the invention
In order to solve the above technical problems, the immunity that the object of the present invention is to provide a kind of by improving broiler chicken, is improved anti-
The feed of disease ability, specific technical solution are as follows:
A kind of broiler fodder, formula is by weight as follows: corn 590-690, dregs of beans 243-285, wheat 70-80, cotton dregs
19-24, calcium monohydrogen phosphate 4.9-5.2, calcium powder 9-11, lysine 3.2-3.8, choline chloride 0.7-1.2, DL- methionine 1.4-
1.5, phytase 0.12-0.15, threonine 0.07-0.09, sodium chloride 2.5-3, bio-additive 2.8-4.7.
The bio-additive is lactic acid bacteria, bacillus subtilis, feed manna oligosacchride, complex enzyme formulation, four
Ratio are as follows: lactic acid bacteria: bacillus subtilis: feed manna oligosacchride: complex enzyme formulation=1-2:0.8-1:0.5-1:0.5-
0.7；
The preparation method is as follows:
(1) powder raw material is after feeding port barrier preliminary screening, into drag conveyor, subsequently into elevator, through circle
Cylinder receiving sieve removal of impurities, pipe magnetic separator are except after iron, by distributor, granularity acceptable material enters batch bin；
(2) granular feedstock is after feeding port barrier preliminary screening, into drag conveyor, subsequently into elevator, through circle
Cylinder receiving sieve removal of impurities, pipe magnetic separator into pulverizer except crushing after iron, and smashed material is through expecting wind auger, elevator, by dividing
Orchestration, into batch bin；
(3) after batch bin raw material preparation is ready, all raw materials enter according to the blanking of recipe requirements dosage and setting sequence
Automatic blending scale enters mixing machine after ingredient, and after mixing 90 seconds -155 seconds, material enters finished bin, through quantitative package
Scale produces packing mixed feed finished product；
(4) premix, the raw material of adding proportion≤0.2% and hand throw additive, and from mixing machine top, hand is thrown mouth and manually added
Add, to ensure that blending process meets technique requirement.
Lactic acid bacteria, bacillus subtilis in the bio-additive need activation in advance, and activation method is: by per kilogram
Additive adds 400-500 grams of brown sugar, 10 kilograms of pure water, after mixing, 24-26 degrees Celsius oxygenation 2-2.5 hours.
The invention has the advantages that bio-additive is lactic acid bacteria, bacillus subtilis, feed manna oligosacchride, complex enzyme
Preparation will not lead to the problem of drug resistance, be green non-pollution additive, and the use of lactic acid bacteria can overcome antibiotic to digestion
The adverse effect of other beneficial microbe colonies in road avoids toxic side effect, drug resistance and drug resistance that antibiotic is used for a long time,
Residual of the drug in animal products is reduced, the quality of animal products is improved, medicament residue is reduced and human health and life is pacified
Full threat.
Specific embodiment
Embodiment 1
Formula is by weight as follows: corn 590, wheat 80, cotton dregs 19, calcium monohydrogen phosphate 5.0, calcium powder 11, relies dregs of beans 260
Propylhomoserin 3.2, choline chloride 1.0, DL- methionine 1.5, phytase 0.12, threonine 0.08, sodium chloride 3, bio-additive
2.8。
The bio-additive is lactic acid bacteria, bacillus subtilis, feed manna oligosacchride, complex enzyme formulation.Four
Ratio are as follows: lactic acid bacteria: bacillus subtilis: feed manna oligosacchride: complex enzyme formulation=1:0.8:0.5:0.5.
The preparation method is as follows:
(1) powder raw material is after feeding port barrier preliminary screening, into drag conveyor, subsequently into elevator, through circle
Cylinder receiving sieve removal of impurities, pipe magnetic separator are except after iron, by distributor, granularity acceptable material enters batch bin；
(2) granular feedstock is after feeding port barrier preliminary screening, into drag conveyor, subsequently into elevator, through circle
Cylinder receiving sieve removal of impurities, pipe magnetic separator into pulverizer except crushing after iron, and smashed material is through expecting wind auger, elevator, by dividing
Orchestration, into batch bin；
(3) after batch bin raw material preparation is ready, all raw materials enter according to the blanking of recipe requirements dosage and setting sequence
Automatic blending scale enters mixing machine after ingredient, and after mixing 90 seconds -155 seconds, material enters finished bin, through quantitative package
Scale produces packing mixed feed finished product；
(4) premix, the raw material of adding proportion≤0.2% and hand throw additive, and from mixing machine top, hand is thrown mouth and manually added
Add, to ensure that blending process meets technique requirement.
Lactic acid bacteria, bacillus subtilis in the bio-additive need activation in advance, and activation method is: by per kilogram
Additive adds 450 grams of brown sugar, 10 kilograms of pure water, after mixing, 24-26 degrees Celsius oxygenation 2-2.5 hours.
Embodiment 2
Formula is by weight as follows: corn 690, wheat 75, cotton dregs 24, calcium monohydrogen phosphate 4.9, calcium powder 10, relies dregs of beans 243
Propylhomoserin 3.8, choline chloride 0.7, DL- methionine 1.45, phytase 0.15, threonine 0.07, sodium chloride 2.8, biology addition
Agent 3.7.
The bio-additive is lactic acid bacteria, bacillus subtilis, feed manna oligosacchride, complex enzyme formulation.Four
Ratio are as follows: lactic acid bacteria: bacillus subtilis: feed manna oligosacchride: complex enzyme formulation=1.5:1:0.5:0.7.
The preparation method is as follows:
(1) powder raw material is after feeding port barrier preliminary screening, into drag conveyor, subsequently into elevator, through circle
Cylinder receiving sieve removal of impurities, pipe magnetic separator are except after iron, by distributor, granularity acceptable material enters batch bin；
(2) granular feedstock is after feeding port barrier preliminary screening, into drag conveyor, subsequently into elevator, through circle
Cylinder receiving sieve removal of impurities, pipe magnetic separator into pulverizer except crushing after iron, and smashed material is through expecting wind auger, elevator, by dividing
Orchestration, into batch bin；
(3) after batch bin raw material preparation is ready, all raw materials enter according to the blanking of recipe requirements dosage and setting sequence
Automatic blending scale enters mixing machine after ingredient, and after mixing 90 seconds -155 seconds, material enters finished bin, through quantitative package
Scale produces packing mixed feed finished product；
(4) premix, the raw material of adding proportion≤0.2% and hand throw additive, and from mixing machine top, hand is thrown mouth and manually added
Add, to ensure that blending process meets technique requirement.
Lactic acid bacteria, bacillus subtilis in the bio-additive need activation in advance, and activation method is: by per kilogram
Additive adds 400 grams of brown sugar, 10 kilograms of pure water, after mixing, 24-26 degrees Celsius oxygenation 2-2.5 hours.
Embodiment 3
Formula is by weight as follows: corn 650, wheat 70, cotton dregs 22, calcium monohydrogen phosphate 5.2, calcium powder 9, relies ammonia at dregs of beans 285
Sour 3.6, choline chloride 1.2, DL- methionine 1.4, phytase 0.14, threonine 0.09, sodium chloride 2.5, bio-additive
4.7。
The bio-additive is lactic acid bacteria, bacillus subtilis, feed manna oligosacchride, complex enzyme formulation.Four
Ratio are as follows: lactic acid bacteria: bacillus subtilis: feed manna oligosacchride: complex enzyme formulation=2:1:1:0.7.
The preparation method is as follows:
(1) powder raw material is after feeding port barrier preliminary screening, into drag conveyor, subsequently into elevator, through circle
Cylinder receiving sieve removal of impurities, pipe magnetic separator are except after iron, by distributor, granularity acceptable material enters batch bin；
(2) granular feedstock is after feeding port barrier preliminary screening, into drag conveyor, subsequently into elevator, through circle
Cylinder receiving sieve removal of impurities, pipe magnetic separator into pulverizer except crushing after iron, and smashed material is through expecting wind auger, elevator, by dividing
Orchestration, into batch bin；
(3) after batch bin raw material preparation is ready, all raw materials enter according to the blanking of recipe requirements dosage and setting sequence
Automatic blending scale enters mixing machine after ingredient, and after mixing 90 seconds -155 seconds, material enters finished bin, through quantitative package
Scale produces packing mixed feed finished product；
(4) premix, the raw material of adding proportion≤0.2% and hand throw additive, and from mixing machine top, hand is thrown mouth and manually added
Add, to ensure that blending process meets technique requirement.
Lactic acid bacteria, bacillus subtilis in the bio-additive need activation in advance, and activation method is: by per kilogram
Additive adds 450 grams of brown sugar, 10 kilograms of pure water, after mixing, 24-26 degrees Celsius oxygenation 2-2.5 hours.
The nutritive index of formula of the invention such as following table
The theory of constitution of inventive formulation is as follows:
Calcium monohydrogen phosphate, white monoclinic system crystal powder, odorless, tasteless.Usually with dihydrate, (its chemical formula is
CaHPO42H2O form) exists.
Phosphorus, the calcium constituent being mainly used in supplement broiler fodder；Promote feed digestion, make poultry weight gain, to increase
Add meat yield.
Choline chloride, English name: Choline chloride
Chinese nickname: chlorination gallbladder rouge, chlorination 2- ethoxy trimethylammonium, choline chloride
Choline chloride can prevent liver, the Fat Accumulation in kidney and its tissue degeneratiaon as feed addictive；It can promote
The recombinant of amino acid；Amino acid, the especially utilization rate of essential amino acid methionine in vivo can be improved.
DL- methionine is a kind of important amino acid kind, is a kind of essential additive in animal feed,
It can promote fast growth of animal added with the feed product of methionine.
Phytase 5000 can be used in phytase, is distant place Zhong Hui Biotechnology Co., Ltd and Mianyang, Sichuan grass biology
Engineering Co., Ltd's cooperation, the biological products researched and developed jointly.Can phytate phosphorus in hydrolysate feed and release Phos, improve
The utilization rate of phosphorus in feed reduces the use of inorganic phosphorous sources, reduces feed formula cost, while can reduce phosphorus in animal wastes
Discharge, protect environment, be a kind of additive for farm animal feed of green high-efficient.
Threonine is a kind of essential amino acid, L-threonine is added in mixed feed, is had the characteristics that as follows: 1.
The amino acid balance of adjustable feed promotes poultry growth；2. meat can be improved；3. the low feeding of amino acid digestibility can be improved
The nutritive value of material；4. feed formula cost can be reduced.
The bio-additive is lactic acid bacteria, bacillus subtilis, feed manna oligosacchride, complex enzyme formulation, be can be used
The product of Wuhan Feng Tian Biotechnology Co., Ltd, packing specification are 1 kilogram every bag, wherein lactic acid bacteria and bacillus subtilis
Need preactivated, the content in formula refers to the net weight of the product of buying.Its effect is as follows:
1 lactic acid bacteria is in the intracorporal mechanism of action of broiler chicken machine
1.1.1 growth promoting function
Synthesize vitamin and mycoprotein
Lactic acid bacteria in the metabolic process can synthesising thalli protein, in microorganism itself lysozyme and broiler chicken gastric acid, stomach egg
Under the effects of white enzyme, trypsase, mycoprotein can be decomposed and be digested and assimilated by Intestine of Broiler；Lactic acid bacteria can also synthesize B race
Vitamin is utilized for broiler chicken body, when B family vitamin deficiency in feed, there is significantly reinforce plus excitometabolic effect.It is yellow
It the report such as celebrates one's birthday, is increased separately with lactobacillus bulgaricus and streptococcus thermophilus fermentation corn flour and wheat flour, lysine content
72% and 85%, methionine increases separately 40% and 46%, and thiamine and riboflavin increased, and nomadic nitrogen increases by 160%
With 40%, free iron increases by 130% and 90%, and free calcium increases by 150% and 120%, and overall nutrition value significantly improves.
1.1.2 promote absorption of nutrient ingredients
In slant acidity environment, the intracorporal some digestive ferments (such as protease) of broiler chicken machine can just be activated, and decompose nutrition
Substance.Lactic acid bacteria generates the good volatile fatty acid of a large amount of lactic acid in the metabolic process, can reduce pH in digestive tract, activation digestion
Enzyme promotes the digestion and absorption of nutriment.Li Lusheng etc. selects 1 age in days of greenhouse fiber crops chicken female young, using basal diet (without containing anti-
Raw element and lactic acid bacteria), it is basal diet+aflatoxin 0.0125%, basal diet+lactobacillus preparation 1.5% (drinking-water), basic
Daily ration+lactobacillus preparation 1.5% (spice post-fermentation 6h).Experimental period is 72d, single cent early period (1~4 week), mid-term (5~8 weeks),
Later period (9~11 weeks), the results showed that, fermentation group can significantly reduce caecum pH, improve duodenum and jejunum amylase activity (P
< 0.05).In addition, pH is reduced in enteron aisle, additionally it is possible to which activated mineral ion (calcium and magnesium ion etc.) promotes animal and bird intestines chats
The digestion and absorption of substance.
1.2 adjust alimentary canal Tiny ecosystem
1.2.1 the physiological effect sticked
Lactic acid bacteria can be colonized in Intestinal epithelial cells surface, and form one layer " biomembrane ", maintain the complete of intestinal mucosa
Whole property, protects body from the damage of external pathogen and harmful substance.In addition, lactic acid bacteria also has by lowering specificity letter
Number transmitting directly affects the effect of immune response.It, can after lactic acid bacteria is attached to animal intestinal tract or when by pathogenic bacterial infection
To activate MAP-kinase pathways and NF-kB approach, to generate immune response, promote multinuclear leucocyte from microvasculature to
Intestines control transfer.And these cells can participate in swallowing and killing the Inoculating microbes of invasion.Can lactic acid bacteria enter enteron aisle and stick
In the mucosa cells surface of host section enteron aisle, form stable dominant microflora, be determine lactic acid bacteria actual effect it is important because
Element.Active bacteria formulation has species specificity to the adhesion of host, the Bacillus acidi lactici that is separated to from a certain animal, Bifidobacterium
There is preferable adhesiveness to this kind of animal alimentary canal epithelium Deng, low adhesion is then presented to other animals or is not sticked.
1.2.3 improve microbial flora
Lactic acid bacteria generates organic acid (lactic acid, acetic acid etc.) in the metabolic process, can reduce the pH in alimentary canal and oxidation
Reduction potential (Eh) makes enteron aisle be in acidic environment.Pathogenic pathogenic microorganism is generally easily in the neutral environment that pH is 6~8
Existence, and lactic acid bacteria generate intestinal acidity environment, for certain pathogenic bacteria (shigella dysenteriae, typhoid bacillus, paratyphosum Bacterium,
Staphylococcus etc.) there is antagonistic action.The hydrogen peroxide of generation can inhibit and kill Gram-negative bacteria, and catalase positive is thin
Bacterium (such as single false spore Pseudomonas), Escherichia coli and Salmonella.Lactic acid bacteria generates the tiny protein or peptide of similar bacteriocin
Class, such as various lactobacillins and Bifidoxin, to staphylococcus, clostridium and salmonella and Shigella
There is antagonistic action, Bifidobacterium etc. can be decomposed into free cholic acid, inhibiting effect of the latter to bacterium with combining cholic acid
It is more stronger than the former.Warm rosy clouds etc. are utilized and are made of chicken manure enterococcus, chicken non-pathogenic escherichia coli, bacillus and lactic acid bacteria
Bacterial Complex feed chick after, immune 4d is being injected intraperitoneally with sheep red blood cell (SRBC), is making to generate hemolysin in Chickens
(IgM), by measuring the potency of hemolysin in its serum, discovery gavages hemolysin potency ratio caused by the chick of different bacterium amounts
Hemolysin potency caused by the control group not gavaged is higher by 22%~32%, and addition lactic acid bacteria has enteron aisle Salmonella
There is apparent inhibiting effect.
1.2.4 poisonous and harmful substance is removed
Lactic acid bacteria can remove the harmful substances such as the endotoxic amine of enteron aisle, sulfide, phenols and indoles, improve intestinal environment,
Promote the fast-growth of broiler chicken.The report such as Li Lusheng, lactic acid bacteria can significantly reduce amine concentration in caecum.
1.3 improve immunity
Lactic acid bacteria can promote the development of immune organ, stimulation specificity and nonspecific immune response, and enhancing body is certainly
Body immunity.
Progress of 1.4 lactic acid bacterias in broiler chicken nutrition
Currently, many large-scale plant that raises are still largely using feeding antibiotic, it will cause bacterial drug resistance and significantly improve.Cream
Sour bacterium is viable bacteria, will not lead to the problem of drug resistance, is green non-pollution additive, and the use of lactic acid bacteria can overcome antibiotic
Adverse effect to other beneficial microbe colonies in alimentary canal, avoid being used for a long time the toxic side effect of antibiotic, drug resistance and
Drug resistance reduces residual of the drug in animal products, improves the quality of animal products, reduce medicament residue to human health and
The threat of life security.
1.4.1 to the influence of growth performance
Miao Xiaowei etc. adds the antibiotic and lactic acid bacteria and Chinese medicine knot synbiotic preparation of equivalent into broiler chicken son's daily ration respectively,
The result shows that the growth performance and efficiency of feed utilization difference of lactic acid bacteria and Chinese medicine symphysis tuple be not significant (P > 0.05).Chen Guixiang
AA broiler chicken 70992 is only randomly divided into four groups Deng under the conditions of large-scale breeding, control group uses antibiotic, and 3 test groups are complete
Not applicable antibiotic entirely, but the lactic acid bacteria of various concentration is added by drinking-water, feed, environment, the results showed that, 3 test groups
Have the tendency that from delivering number of elements, survival rate for sale, delivering average weight for sale higher than antibiotic group, meat feed ratio is below antibiotic group (P <
0.05).The results of study such as Li Lusheng show fermentation method addition lactic acid bacteria group and antibiotic group in production performance and immunity function
Aspect increases than drinking-water group without significant difference (P > 0.05).Qu Xiangyong etc. selects 21 age in days yellow-feather broiler 180,
Three groups are randomly divided into, respectively addition lactobacillus preparation 0,0.01%, 0.02%, experimental period 2 weeks, the results showed that, add lactic acid bacteria
0.02% group of average daily gain of agent is significantly higher than control group (P < 0.05)；Difference is not significant (P > 0.05) during 2 tests, examination
A group feed conversion ratio control group difference low 8.75% and 15.62% is tested, lactobacillus preparation consumption can obviously reduce meat than control group
The cost of chicken feed production.Two subspecies of lactobacillus reuteri and curling cream in the tangible young screw daily ration of broiler of 1 age in days male of Li Ming
Bacillus, the quantity 107cfug-1 of microorganism in feed, gross protein value 22.20% in feed, the results showed that, addition cream
Sour bacteria preparation has positive effect (P < 0.05) to the weight gain of Broiler chicks, and food conversion is hastily slightly after adding lactic acid bacteria
Increasing, intake and feed conversion rate difference is not significant (P > 0.05), after 1 week old Broiler chicks Pu Chong lactic acid bacteria, lactic acid in caecum
The quantity of bacterium increases, but difference is not significant (P > 0.05), and the quantity variance of saccharomycete is not significant (P > 0.05) in caecum, feeding
Lactic acid bacteria can increase Broiler chicks weight and improve the quantity of lactic acid bacteria in caecum.
1.4.2 to the influence of immune function
Bacillus acidi lactici can promote the development of broiler chicken immune organ.Yi Li etc. is the study found that Bacillus acidi lactici can significantly improve
The index and spleen index and bursal index (P < 0.05) of your 21 age in days of chicken of Condar, improve the Thymus and Spleen index of 42 ages in days,
It can be improved body non-specific immune function.Hang Bailin etc. adds lactobacillus plantarum and 2 strains of lactic acid bacteria of streptococcus fecalis respectively
Into broiler fodder, the results showed that, 2 strains of lactic acid bacteria can promote the development of broiler chicken thymus gland, spleen and the bursa of farbricius, enhance cell
Phagocytic function, increase the T cell number in thymus gland and spleen, improve the garland formation rate of E-C3bR and E-ICR, improve body
ND-HI antibody level.
1.4.3 antibacterial action
Lactic acid bacteria can generate inhibiting effect to harmful bacteria by the growth and breeding of itself and its metabolin of generation.Han Qiuxia
Deng the bacteriostasis for studying broiler chicken source lactic acid bacteria under conditions in vitro, the results showed that, Escherichia coli Growth is by different degrees of suppression
System, gained bacterial sediment acts on Escherichia coli unrestraint after the centrifugation of lactic acid bacteria bacteria suspension, and the extracellular metabolism in supernatant produces
Object has obvious bacteriostasis, in supernatant bacteriostasis about half it is above be to be generated by lactic acid bacteria, remaining fungistatic effect then comes
Other antibacterial substances generated derived from lactic acid bacteria.Guo Jinling etc. has studied lactic acid bacteria, lactic acid bacteria supernatant, antibiotic and MRS pairs
According to liquid to the external In Vitro Bacteriostasis of the therapeutic Escherichia coli of chicken, the results showed that, it is sterile after Bacillus acidi lactici bacterium solution and culture
Supernatant has stronger bacteriostatic activity, and the diameter of inhibition zone is noticeably greater than control group (P < 0.05)；And as lactic acid bacteria is trained
The difference of time is supported, the pH of lactic acid bacteria culture solution is gradually decreased, also gradually increased to the inhibiting effect of enteropathogenic E. Coli.Horse
Space etc. is controlled using to broiler feeding lactic acid bacteria and its culture solution, detecting it influences Intestine of Broiler flora, the results showed that, 14,
28, the increasing of escherichia coli in caecum can be significantly inhibited by adding lactic acid bacteria bacterium powder and lactic acid bacterial liquid when 35,42 age in days in daily ration
It grows (P < 0.05), increases the quantity (P < 0.05) of lactic acid bacteria and Bifidobacterium；Raffinate and inactivated bacterial liquid are cultivated generally to blind
Intestinal flora does not make significant difference (P > 0.05).
2 bacillus subtilises
Bacillus subtilis is widely distributed in different living environments, generally existing in soil and plant surface, it can be with
Generate endospore, resistance is strong, in animal productiong using wide.
2.1 adjust intestinal microecology balance
Bacillus subtilis can consume rapidly the free oxygen in environment, cause enteron aisle hypoxemia, promote to grow beneficial to anaerobic bacteria,
And the organic acids such as lactic acid and protein and peptide antibacterial material are generated, inhibit the breeding of enteropathogenic bacterial growth, improves beneficial to micro- life
Competitive advantage (Hu Dongxing etc., 2001) of the object in enteric bacteria inter-species.
The 2.2 a variety of enzymes of secretion, improve animal digestion enzymatic activity
Bacillus subtilis can secrete a variety of enzymes, have stronger protease, amylase and lipase active, also
Pectase, dextranase etc. decompose the enzyme of non-starch polysaccharide in plant feed, they play work with endogenous enzymes in alimentary canal jointly
With raising animal digestion enzymatic activity improves efficiency of feed utilization.
2.3 generate various nutrients, participate in body metabolism
Bacillus subtilis growth and breeding in animal intestinal tract can generate a variety of nutrients such as vitamin, somatomedin
Matter can also improve animal to the utilization rate of calcium, phosphorus, participate in the metabolism of body.
2.4 improve animal immunizing power
Bacillus subtilis can stimulate the growth and development of animal immune organ, and activated lymphocyte improves immune globulin
White and antibody level activates the immune defense system of whole body by Lymphatic Circulation, improves animal immunizing power.
Application of 2.5 bacillus subtilises in poultry production
2.5.1 application of the bacillus subtilis in daily ration of broiler
Apply the research on broiler breeding many bacillus subtilis, addition probiotic can improve broiler chicken survival rate,
Improve weight gain and feed efficiency.Pan Kangcheng etc. (2005) studies bacillus subtilis formulation to growth of meat chicken, trunk and chicken product
The influence of matter, the results showed that test latter stage average weight, net gain, daily gain and food conversion can be improved in bacillus subtilis
Rate, chicken muscle total amino acid content, essential amino acid and Fresh ear field content increase.
A.Y-L.Teo and H-M.Tan (2006) selects 1 age in days Broiler chicks 600, is randomly divided into 3 groups, respectively compares
Group, antibiotic processing group and bacillus subtilis processing group, every group is divided into 2 processing A and B again.3 processing groups are to B processing
Broiler chicken carries out challenge with E.coli.From test result it follows that in 21 ages in days and 42 age in days, bacillus subtilis processing group
Two processing weight gains of A and B are better than antibiotic treatment group, are significantly higher than Escherichia coli processing group.Feed conversion rate relatively in, it is withered
Careless bacillus feed conversion rate in three groups be also it is optimal, significant difference (p < 0.05), see Table 1 for details.
Influence of 1 different disposal of table to 21 ages in days and 42 Day-old Broiler Chickens weight gain and feedstuff-meat ratio
Note: different shoulder marks indicate significant difference (p < 0.05) in same column
Bacillus acidi lactici is intrinsic beneficial bacterium in enteron aisle, has very important effect to the healthy growth of animal body.It is in table 2
Influence of the different disposal to Bacillus acidi lactici content in 32 ages in days and 42 age in days Jejunal chymes, data show bacillus subtilis processing
Bacillus acidi lactici content is higher in group Jejunal chyme, illustrates that bacillus subtilis has supporting role to Bacillus acidi lactici, to inhibit big
The harmful bacterias such as enterobacteria colonize.
Influence (log cfu/g) of 2 different disposal of table to Bacillus acidi lactici content in 32 ages in days and 42 age in days Jejunal chymes
3 feed manna oligosacchrides
Manna oligosacchride (Mannose-oligosaccharides, MOS), refer to by mannose and glucose group at oligosaccharides,
Pass through α (1-6), α (1-2) or α (1-3) glucosides key connection.Feed MOS be mainly derived from yeast cell wall extract (Mul and
Perry,1994)。
The metabolic characteristic of MOS feed addictive is: being not the decomposition of nonruminant digestive enzyme.It is formed sediment with ptyalin, pancreas
Based on the disaccharidases such as maltose, invertase, lactase on powder enzyme and mucous membrane of small intestine, starch, the sugar in feed are decomposed
Original, sucrose, lactose are that the monosaccharide forms such as glucose, fructose, galactolipin are utilized for body, this is almost exactly that monogastric mammal disappears
Change whole abilities that road enzyme decomposes polysaccharide.MOS and other functional oligosaccharides are similar with many feed fibres, all contain and largely cannot
It for the chemical bond that digestive enzyme interrupts, is hardly digested in small intestine and is concentrated using and into alimentary canal back segment, and be animal
Archenteric flora (predominantly alimentary canal back segment flora) selective fermentation utilizes, and is released in the form of organic acid, CH4, CO2, H2
Or metabolism is participated in, energy is provided.
The biological function of feed manna oligosacchride is as follows:
3.1, by optimization intestinal microecology system, beneficial healthcare function are played to host
3.1.1MOS optimize the mechanism of action of intestinal microecology system
MOS is added in feed, can be improved enteric microorganism composition, be formed using Bifidobacterium and Bacillus acidi lactici as dominant bacteria
Intestinal flora.It is reported that (Alltech, 1994), MOS or other oligosaccharides can be used as a kind of nutriment of growth metabolism,
It is utilized by Bifidobacterium and Bacillus acidi lactici selective fermentation, and promotes its growth and breeding.And Escherichia coli, Salmonella, production
The pathogenic bacterium such as gas Clostridium but leads to hungry death because that can not utilize.Some newer researchs are also believed that oligosaccharides not only
Serve as a kind of carbon source or nutriment, prior effect is the effects of participating in the growth regulating and adherency of Bifidobacterium, these
Still up for further studying.
3.1.2 to the helpful function of host
It is by being formed using Bifidobacterium and Bacillus acidi lactici as the intestinal flora of dominant bacteria to the helpful function of host
And realize.It has been recognized that Bifidobacterium, which has, improves body antibody level, promotes the phagocytic activity of macrophage and disappear
Change ability improves body resistance infection and prevention, the effect of killing tumor cell；By reducing gut pH or passing through bifid bar
Shielding Competition of the bacterium to unfavorable bacterium in space, nutrition, to inhibit harmful bacteria such as spoilage organisms, pathological form Escherichia coli, will
The field planting and growth in enteron aisle such as Hayes shigella dysenteriae；Promote being absorbed and utilized for various minerals such as Ca, Fe, Mg, Zn, reduces blood
The level of clearing gallbladder sterol and triglycerides；Bifidobacterium, which also has, eliminates free radical, hydroxy radical, peroxide lipid and corruption carefully
Indoles, the amine, phenols of bacterium generation, reduce the helpful function of enteron aisle smell etc.；Vitamin B1, vitamin B2, dimension can be synthesized
The multivitamins such as raw element B6, vitamin B12, niacin and folic acid, and can control endotoxemia (Wakiguchi, 1985)；Energy
Prevent anaemia and rickets, the formation (Hoover, 1993) of the harmful substances such as nitrite can be reduced.Bacillus acidi lactici is in this side
Face also has similar function, they are the considerable advantage floras for maintaining intestinal health.
Report there is no to confirm at present, addition MOS, which has, promotes being absorbed and utilized, reducing serum cholesterol and glycerol for minerals
The healthcare function of three ester levels etc.；And it is also seldom to directly research the document that MOS adjusts Tiny ecosystem, and there are disagreements.Such as:
Spring (1995) proposes that MOS cannot even be degraded by enteric bacteria.From at present to Bifidobacterium glycosidase research conditions from the point of view of,
Have no the enzyme (Su Wenjin etc., 1996) of obvious degradation MOS.But also there is test to confirm that MOS can promote Bifidobacterium and lactic acid bar
The growth (Alltech, 1994) of bacterium.
Identification, adherency and the excretion of 3.2 pairs of pathogenic microorganisms
Pathogen is by the agglutinin of pathogen surface specific, with place in the specific adhesion of mucosal epithelial surface
What the main corresponding saccharide acceptor interaction of mucomembranous epithelial cell was completed.In addition to Salmonella remains query, most pathogens
It is pathogenic to be stained with biggish correlation (White etc., 1978) with its specific mucosal.This mainly due under health status,
Immunologic mechanism that animal infects there are local Anti-bacterium is (the washing away of mucus and urine, the wriggling of intestinal villus, local antibody, molten
Bacterium enzyme etc.), if not by this specific adhesion, germ is difficult to be colonized and be excreted on mucosal epithelial surface.
Most enteric bacteria can be colonized on mucomembranous epithelial cell by mannose specific agglutination element.Research table at present
On bright film contain D-MANNOSE, beta galactose, L-fucose, the compound of the sugar such as N- acetylamino galactosamine or sialic acid, it
All can serve as receptor in conjunction with pathogen agglutinin.And mannose specific agglutination element is since its is widely distributed, by
Pay attention to.(salmonella typhimurium, enteritis are husky for most enteric bacteria such as Escherichia coli (O6, O5, O2, O125), Salmonella
Men Shi bacillus, Bacillus paratyphosus B, typhoid bacillus), Klebsiella, shigella flexneri etc. all have sweet dew sugar specificity
Agglutinin is distributed mainly in pili (I type), and minority is distributed on flagellum or outer envelope.About this point, Zhou etc.
(1995), Oyofo (1989), Ofek (1977), Lindquist (1987), Sharon (1990) etc. are by utilizing D-MANNOSE
And its in vitro test that derivative inhibits germ to stick, verifying is given indirectly.Eshdat, Karan, Klemm etc. are then directly adopted
Direct verifying is given with advanced biochemical technology.
The MOS of feed small can with containing the receptor of mannose, it is sweet to compete pathogenic microorganism in intestinal epithelial cell
Reveal the binding site of sugar specificity agglutinin.Overview thinks that MOS is saturated in conjunction with pathogen agglutinin in conjunction with rear MOS
Site in conjunction with pathogen agglutinin receptor corresponding to intestinal epithelial cell, hinders the Mucoadhesive of pathogen, and due to
MOS has non-digestible, and this compound can pass through gastrointestinal tract and be excreted (Newmman, 1994).It could also be possible that
After MOS and pathogen combine, pathogen can not be hungry dead using the nutrition that it is provided.In addition think there are also a kind of view,
Feed MOS is by the binding site of pathogenic microorganism on closing intestinal epithelial cell, to prevent its Mucoadhesive.It is interesting
Be Ofek (1977) the study found that when methyl α-D-MANNOSE glycosides that 6 or 12mg/mL is added, can make to be attached on mucous membrane thin
Escherichia coli on born of the same parents have 25% dissociation in 2~5min.Duguid and Old (1980) thinks that this can for this phenomenal research
Can further increase pili hydrophily in the hydrophilic group for can be incorporated into pili due to mannose, promote its cell surface
Fall off.
3.3 immunomodulator
At present it has been recognized that the nospecific immunity of animal can be enhanced in MOS, this respect is still up for further grinding
Study carefully.
3.3.1 auxiliary agent activity
For a long time, about the strong auxiliary agent activity of the linear mannosan side chain of lipopolysaccharides LPS, widely recognized
It can.On this basis, Sharon and Lis (1993) are researched and proposed, and MOS can not only be connected on bacterium, and can be connected to poison
On element, viral, eucaryote, it can be used as the auxiliary agent of these exogenous antigens in conjunction with rear MOS, alleviate the absorption of antigen, enhance
The cellular immunity and humoral immunity of body.As prestige Animal Health Care Products Corporation of America and Soviet Union using aloe development and production " acetylated mannan is poly-
Sugar " product has had been widely recognized in husbandry sector practice as the adjuvant of Marek's vaccine.
3.3.2 promoting hepatic secretion mannose binding protein
Janeway (1993) research thinks, the oligosaccharides containing mannose can by stimulate hepatic secretion mannose binding protein,
Mannose binding protein is calcium dependent form agglutinin, main to combine mannose to pass through activation in animal immune vulnerable period by identification
Complement system plays the function of conditioning and natural anti-infectious immunity, and mannose binding protein exists in conjunction with zymosan without Clq
Clr2, Cls2 can be activated.According to another report, zymosan can direct activation C3, pass through alternative pathway activating complement system.
3.3.3 MOS can also be by interacting (Chesson, 1993) with intestinal villus immunocyte surface protein receptors
Or by intervene the signal system that is present on lymph node and proper mucous membrane memory cell carry out immunological regulation (Lessard and
Brisson, 1987).Studies have shown that in feed add MOS can be enhanced phagocyte phagocytic activity (Alltech, 1994；
1995)。
4 complex enzyme formulations
4.1 effective decomposition nutrition matter improve rate of body weight gain, reduce feed coefficient
Most of diet is mainly made of vegetable raw materials such as cereal, puffed soybeans, and cell wall in these vegetable raw materials
Presence reduce the digestion and absorption of nutrient.Plant cell wall is by a variety of polymer compounds (cellulose, hemicellulose and pectin etc.
Non-starch polysaccharide (non-starch polysaccharides, NSP)) composition, also contain a small amount of protein, phenols and fat
The substances such as acid.These substances are cross-linked with each other by hydrogen bond or covalent bond in conjunction with cell wall structure is collectively formed, and support and protection is thin
The content of born of the same parents.Since fish body digestive system cannot secrete zytase, cellulase etc., so adding exogenous enzymes in feed
Preparation (such as cellulose decomposition enzyme) can effectively break the cell wall of vegetality feedstuff, make intracellular nutriment
(starch, protein and fat etc.) releases, and is allowed to sufficiently have an effect with alimentary canal endogenous enzymes, to improve feed conversion
Rate, and then improve the production performance of fish body.
4.2 eliminate anti-nutritional factors, reduce chyme viscosity, improve feed digestibility
NSP rich content present in mixed feed vegetable raw material, including it is araboxylan, beta glucan, penta poly-
Sugar, cellulose and pectic substance etc., these macromolecular substances can absorb a large amount of moisture, or make chyme in enteron aisle after being dissolved in water
Viscosity enhancing；Meanwhile NSP can also be such that the motionless water layer of intestinal mucosal surface thickens, and cause nutriment diffusion hindered, under absorptivity
Drop, influences digestion and absorption of the fish to effective nutritional ingredient.Add exogenous enzyme preparations (such as zytase, 1,4 beta-glucanase, fruit
Carbohydrase and cellulase etc.), under the synergistic effect of endogenous enzymes, cellulose, pectic substance and glycoprotein can be degraded to monosaccharide
And oligosaccharides, the viscosity of alimentary canal content is reduced, the obstacle that substance of this kind utilizes animal digestion, the row of accelerating are reduced
Air speed degree increases the digestion and absorption of nutrient, improves the digestive function of fish.
The deficiency of the 4.3 endogenous digestive ferments of supplement, promotes Nutrients Digestion to absorb
The activity of different growth phase its digestive ferment of poultry has apparent difference, especially young alimentary canal and enzyme system
Development it is very not perfect, principal digestive enzymes hyposecretion, far from meet its decompose macro-nutrients needs, this is just
Exogenous digestive ferment, which plays a role, to be created condition, and the addition of exogenous enzyme preparations can provide more for the matrix of decomposition to stimulate
The secretory volume of endogenous enzymes, to improve the digestibility of feed.
4.4 promote the digestion and absorption of nitrogen phosphorus, reduce environmental pollution
Enzyme preparation can improve the metabolism of body as a kind of metabolism regulators, improve the utilization rate of nutrient, especially
It is the effective use of nitrogen phosphorus, and then reduces the excretion of nitrogen phosphorus, efficiently reduce the pollution to water quality, promotes the strong of aquatic livestock
Health improves the disease resistance of aquatic livestock, such as phytase.
4.5 improve the Immunoresistance of poultry, promote animal health
The one kind of enzyme preparation as protein is the natural products of microbial fermentation, nontoxic, without side-effects.Meanwhile enzyme bacterium
The overall absorption of nutriment can be promoted by secreting out of some vitamins, mineral element, protein etc. during the fermentation, enhance house
The constitution and disease resistance of fowl.Addition exogenous enzyme preparations improve poultry to the utilization rate of protein and other nutritional ingredients, promote
Into immunoglobulin, the biosynthesis of lymphokine, prostaglandin immune factor, to improve the immune function of animal.
4.6 improve intestinal flora state.
Claims (2)
1. a kind of broiler fodder, it is characterised in that formula is by weight as follows: corn 590-690, dregs of beans 243-285, wheat 70-
80, cotton dregs 19-24, calcium monohydrogen phosphate 4.9-5.2, calcium powder 9-11, lysine 3.2-3.8, choline chloride 0.7-1.2, DL- first sulphur ammonia
Sour 1.4-1.5, phytase 0.12-0.15, threonine 0.07-0.09, sodium chloride 2.5-3, bio-additive 2.8-4.7.
The bio-additive is lactic acid bacteria, bacillus subtilis, feed manna oligosacchride, complex enzyme formulation；Four ratio
Are as follows: lactic acid bacteria: bacillus subtilis: feed manna oligosacchride: complex enzyme formulation=1-2:0.8-1:0.5-1:0.5-0.7；
The preparation method is as follows:
(1) powder raw material is after feeding port barrier preliminary screening, into drag conveyor, subsequently into elevator, through at the beginning of cylinder
Cleaning removal of impurities, pipe magnetic separator are except after iron, by distributor, granularity acceptable material enters batch bin；
(2) granular feedstock is after feeding port barrier preliminary screening, into drag conveyor, subsequently into elevator, through at the beginning of cylinder
Except being crushed after iron into pulverizer, smashed material passes through distribution through expecting wind auger, elevator for cleaning removal of impurities, pipe magnetic separator
Device, into batch bin；
(3) after batch bin raw material preparation is ready, all raw materials are according to the blanking of recipe requirements dosage and setting sequence, into automatic
Burden balance enter mixing machine after ingredient, and after mixing 90 seconds -155 seconds, material enters finished bin, raw through ration package scale
Output dispenses mixed feed finished product；
(4) premix, the raw material of adding proportion≤0.2% and hand throw additive, and from mixing machine top, hand is thrown mouth and manually added, with
Ensure that blending process meets technique requirement.
2. broiler fodder according to claim 1, it is characterised in that: lactic acid bacteria, withered grass bud in the bio-additive
Spore bacillus needs activation in advance, and activation method is: adding 400-500 grams of brown sugar, 10 kilograms of pure water by per kilogram additive, mixes
Uniformly after, 24-26 degrees Celsius oxygenation 2-2.5 hours.
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| Application Number | Priority Date | Filing Date | Title |
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ID=65303533
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112715777A (en) * | 2020-12-30 | 2021-04-30 | 贵州宏扬饲料有限公司 | Feed for broiler chickens and preparation method thereof |
| CN112970965A (en) * | 2021-03-23 | 2021-06-18 | 四川省畜牧科学研究院 | Sichuan mountain black-bone chicken feed and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105076898A (en) * | 2015-10-10 | 2015-11-25 | 江苏康威科技饲料有限公司 | Eco-nutritional compound feed for laying hens at egg-laying peak and preparation method of eco-nutritional compound feed |
| CN106071305A (en) * | 2016-06-15 | 2016-11-09 | 环山集团有限公司 | A kind of reduce feedstuff broiler chicken batch just and production method |
-
2018
- 2018-12-12 CN CN201811515492.2A patent/CN109329583A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105076898A (en) * | 2015-10-10 | 2015-11-25 | 江苏康威科技饲料有限公司 | Eco-nutritional compound feed for laying hens at egg-laying peak and preparation method of eco-nutritional compound feed |
| CN106071305A (en) * | 2016-06-15 | 2016-11-09 | 环山集团有限公司 | A kind of reduce feedstuff broiler chicken batch just and production method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112715777A (en) * | 2020-12-30 | 2021-04-30 | 贵州宏扬饲料有限公司 | Feed for broiler chickens and preparation method thereof |
| CN112970965A (en) * | 2021-03-23 | 2021-06-18 | 四川省畜牧科学研究院 | Sichuan mountain black-bone chicken feed and preparation method thereof |
| CN112970965B (en) * | 2021-03-23 | 2023-07-14 | 四川省畜牧科学研究院 | Sichuan mountain black-bone chicken feed and preparation method thereof |
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