CN110683655A - Underwater bacterium distribution process for eliminating black and odorous river channel - Google Patents
Underwater bacterium distribution process for eliminating black and odorous river channel Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
- C02F11/04—Anaerobic treatment; Production of methane by such processes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/007—Contaminated open waterways, rivers, lakes or ponds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
Abstract
The invention discloses an underwater bacterium distribution process for eliminating black and odorous riverways, relates to the technical field of water purification, and solves the problem that the existing reagent can only eliminate single pollution and cannot thoroughly eliminate the black and odorous riverways. The invention comprises the following steps: (1) and (3) strain culture: culturing composite anaerobic bacteria, composite aerobic bacteria and composite photosynthetic bacteria, wherein the mass fraction of the cultured bacteria liquid is 1000 parts, and the concentration is 11 ppm; (2) adding in a river: the strain distribution pipe device is used for adding strains into the river through a water pump, the water inlet depth of the strain distribution pipe is adjusted according to the water depth, anaerobic bacteria, aerobic bacteria and photosynthetic bacteria strains are sequentially added into the river, and then the strains are uniformly stirred. The invention has the advantages of reducing the odor of the river water, reducing the total nitrogen content of the river water and improving the color of the river water.
Description
Technical Field
The invention relates to the technical field of river water purification, in particular to an underwater bacterium distribution process for eliminating black and odorous river channels, which is used for improving water quality, reducing odor, reducing nitrogen content in water and the like.
Background
In recent years, along with the rapid development of economy in China, a large amount of urban domestic sewage and industrial waste liquid are discharged, the accumulation of excessive organic matters exceeds the bearing capacity of part of river channels, so that river water is eutrophicated, dissolved oxygen is unbalanced, anaerobic microorganisms propagate in large quantities, malodorous gases such as ammonia and hydrogen sulfide and black substances such as ferrous sulfide and manganese sulfide are generated, and the black and odorous phenomenon begins to appear in water bodies. The black and odorous state is an extreme state of the water body, and the characteristics of the black and odorous state are greatly different from other states. The physical and chemical environment of the ecological floating bed is expressed as strong reducing property, the organic and inorganic pollution is extremely serious, the water body has peculiar smell and is not suitable for the survival of aquatic organisms, the aquatic vegetation is degraded and even died, and the phytoplankton, the zooplankton and the benthonic animal only have a small amount of pollution-resistant seeds. Food chain breakage, food net support breakage, serious unbalance of an ecological system structure and serious degradation and even loss of functions. The black and odorous water in the river channel not only affects the urban image, but also causes great harm to the quality of coastal buildings and the health of residents.
At present, the treatment method of the black and odorous water in the riverway mainly comprises a physical method, a chemical method and a biological method. The physical method mainly comprises the means of dredging, flowing water drainage, aeration reoxygenation and the like, but more projects, equipment and energy consumption are needed, the cost is higher, and the biological method is still needed for radical treatment in the later period. The chemical method adopts the addition of chemical reagents for quick repair, common reagents comprise hydrogen peroxide, calcium peroxide and calcium nitrate, but the conventional reagents usually treat the symptoms and do not treat the root causes in practical application, and secondary pollution is easily caused. The biological method is to degrade organic pollutants in water by using the metabolic activity of organisms (including microorganisms, plants and animals), so as to fundamentally solve the problem of black and odorous water and restore the ecological system of a river channel. A microbial ecological agent belongs to a biological method, and is characterized in that microbes are prepared into liquid or solid powdery products by an expanding culture technology, the liquid or solid powdery products are added into a polluted water body in a certain mode, and pollutants in the water are degraded through the metabolic activity of the microbes, so that the water quality is improved. The microecological preparation is favored because of simple preparation, convenient use, low cost, obvious effect and no secondary pollution, and is gradually the key point of research.
However, the components of the black and odorous water in the river are complex, the black and odorous water in the river cannot be thoroughly removed by using a microecological preparation prepared by a single bacterial strain or a certain type of bacterial strain, and the universality of different black and odorous water in the river is difficult to realize.
Disclosure of Invention
The invention aims to: the invention provides an underwater bacterium distribution process for eliminating black and odorous river channels, and aims to solve the problems that the existing reagent can only eliminate single pollution and cannot thoroughly eliminate the black and odorous river channels.
The invention specifically adopts the following technical scheme for realizing the purpose:
an underwater bacterium distribution process for eliminating black and odorous riverways comprises the following steps:
(1) and (3) strain culture: respectively culturing composite anaerobic bacteria liquid, composite aerobic bacteria liquid and composite photosynthetic bacteria liquid, wherein the mass fraction of the cultured bacteria liquid is 1000 parts, and the concentration is 11 ppm;
the composite anaerobic strain is a mixed strain of 10-20% of clostridium butyricum, 10-20% of green thiobacillus, 15-25% of lactobacillus acidophilus, 15-25% of lactobacillus plantarum, 10-20% of streptococcus faecalis and 10-20% of pediococcus acidilactici;
the composite aerobic bacteria are mixed strains of 10-20% of thiobacillus denitrificans, 10-20% of bacillus subtilis, 10-20% of bacillus licheniformis, 10-20% of bacillus coagulans, 10-20% of bacillus cereus and 20-30% of candida utilis;
the composite photosynthetic bacteria comprise 5-10% of rhodococcus sphaeroides, 80-90% of rhodopseudomonas palustris and 5-10% of rhodopseudomonas capsulata.
(2) Adding in a river: adding strains into the river by using a strain distribution pipe device through a water pump, adjusting the water inlet depth of the strain distribution pipe according to the water depth, sequentially adding anaerobic bacteria, aerobic bacteria and photosynthetic bacteria strains into the river, and then uniformly stirring the strains;
preferably, the composite anaerobic bacteria liquid in the step (1) comprises 2 parts of anaerobic bacteria, 38 parts of culture medium A and 960 parts of water;
preferably, the carbon source, nitrogen source and phosphorus source of medium A are sucrose, peptone, yeast and KH, respectively2PO4The optimum growth factor is tomato juice, and the optimum addition amount is determined by dumpling steaming experiment to be 2.0% of sucrose, 1.5% of peptone, 0.5% of yeast and KH2PO40.2% and tomato juice 5%.
Preferably, the composite aerobic bacteria liquid in the step (1) comprises 0.2 part of aerobic bacteria, 24.5 parts of a culture medium B and 975.3 parts of water;
preferably, the carbon source, nitrogen source and phosphorus of medium BThe sources are corn flour, peptone and KNO respectively3The best growth factor is beef extract, and the formula of the culture medium B is determined to be 7.5g/L corn flour and 15g/L, KNO g/peptone by a two-level Plackett-Burman test31g/L beef extract 7.5g/L, NaCl 5g/L, CaCO g30.75g/L、ZnSO40.7g/L and H2O0.0075g/L。
Preferably, the composite photosynthetic bacteria bacterial liquid in step (1) comprises 1 part of photosynthetic bacteria, 34 parts of culture medium C and 965 parts of water.
The specific culture steps are as follows:
a. the inner wall of the container, a stirring rod, a bubbled stone, a heating rod and the like which are required to be contacted with all the culture medium liquid are sterilized by potassium permanganate, but potassium permanganate residues cannot exist;
b. the water is disinfected before use, treated by bleaching solution, and then the residual bleaching solution is removed by using sodium bicarbonate, so that no bleaching solution residue is left in the water;
c. adding water into a water tank for culturing strains, heating the water to 36-42 ℃, safely taking out a heating rod, and turning on an indoor heat source to keep the room temperature at 45 ℃;
d. c, putting the culture medium into the water heated in the step c, uniformly stirring for about 30min, adding the prepared composite anaerobic bacteria, composite aerobic bacteria or composite photosynthetic bacteria, and respectively culturing the three strains;
e. and d, stirring the aqueous solution mixed with the strains and the culture medium in the step d for about 5min every 2h, continuing for 14h, turning off an indoor heat source, opening a door in a greenhouse for cooling, and finishing the propagation.
The invention has the following beneficial effects:
1. anaerobic compound strains are added, substrate sludge decomposition and organic micromolecule decomposition are continuously carried out, harmful bacteria such as escherichia coli and the like generated by volatile odor are inhibited, and odor is reduced; adding aerobic composite strains to directly or indirectly promote the indigenous bacteria to continuously carry out ammoniation, nitrification and denitrification processes, so as to reduce the total nitrogen content; the composite photosynthetic bacteria are added, the photoautotrophic bacteria are utilized to tolerate high-concentration organic wastewater and have strong decomposition and conversion capacity, hydrogen sulfide is converted, the composite photosynthetic bacteria are further subjected to small molecular modification, and are matched with algae, the total biomass of a water body is improved, the transparency of the surface layer of the water body is increased, the photosynthesis of phytoplankton is promoted, and the water color appearance is improved.
2. If the temperature is lower than 36 ℃, the strain is slowly propagated and is easy to infect mixed bacteria; if the temperature is higher than 42 ℃ for a long time, uniform production and propagation are not facilitated, even the strains die, the method disclosed by the invention can be used for cultivating the strains at the temperature of 36-42 ℃, so that the normal growth of the strains is ensured, and the effect of removing the black and odorous river channels is prevented from being influenced by the occurrence of mixed bacteria pollution; and as the germination period of the strain is 6-8 h, the logarithmic period is 1-2 h and the stationary period is 1-4 h, the viable count reaches the highest peak after 14h of culture, the strain activity is vigorous, and the using effect is optimal.
3. Because the bacteria liquid feeding in the river channel is basically that workers manually feed the bacteria liquid on a ship, the bacteria feeding is uneven, and particularly, the bacteria liquid in the deep water part is little, the bacteria tube distributing device is adopted for feeding the bacteria, and the bacteria tube distributing device can be lengthened or shortened according to the depth of the river channel, so that the bacteria tube distributing device is suitable for different depths of the river channel, and the uniformity of the bacteria liquid feeding is effectively improved; and branch pipes can be arranged on the side surfaces of the four-way joints according to the pollution condition of a river channel and the bacterium feeding amount, so that the bacterium feeding range is expanded and the bacterium feeding speed is increased.
Detailed Description
In order that those skilled in the art will better understand the present invention, the following examples are provided to illustrate the present invention in further detail. The following detailed description of embodiments of the invention is not intended to limit the scope of the invention as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The embodiment provides an underwater bacterium distribution process for eliminating black and odorous riverways.
The composite anaerobic strain is a mixed strain of 15% clostridium butyricum, 15% green thiobacillus viride, 20% lactobacillus acidophilus, 20% lactobacillus plantarum, 15% streptococcus faecalis and 15% pediococcus acidilactici.
The carbon source, the nitrogen source and the phosphorus source of the culture medium A of the composite anaerobic bacteria are respectively sucrose, peptone, yeast and KH2PO4The optimum growth factor is tomato juice, and the optimum addition amount is determined by dumpling steaming experiment to be 2.0% of sucrose, 1.5% of peptone, 0.5% of yeast and KH2PO40.2% and tomato juice 5%.
The composite aerobic bacteria are mixed strains of 15 percent of denitrogenated thiobacillus, 15 percent of bacillus subtilis, 15 percent of bacillus licheniformis, 15 percent of bacillus coagulans, 15 percent of bacillus cereus and 25 percent of candida utilis.
The carbon source, the nitrogen source and the phosphorus source of the culture medium B of the composite aerobic bacteria are respectively corn flour, peptone and KNO3The best growth factor is beef extract, and the formula of the culture medium B is determined to be 7.5g/L corn flour and 15g/L, KNO g/peptone by a two-level Plackett-Burman test31g/L beef extract 7.5g/L, NaCl 5g/L, CaCO g30.75g/L、ZnSO40.7g/L and H2O 0.0075g/L。
The composite photosynthetic bacteria comprise mixed strains of 7.5 percent of rhodococcus sphaeroides, 85 percent of rhodopseudomonas palustris and 7.5 percent of rhodopseudomonas capsulata, and the culture medium C can adopt the existing composite photosynthetic bacteria culture medium.
The composite anaerobe liquid comprises 2 parts of anaerobe, 38 parts of culture medium A and 960 parts of water.
The specific culture steps are as follows:
a. the inner wall of the container, a stirring rod, a bubbled stone, a heating rod and the like which are required to be contacted with all the culture medium liquid are sterilized by potassium permanganate, and potassium permanganate residues cannot exist after the sterilization;
b. the water is optimally cooled boiled water, if the water is not cooled boiled water, the water is required to be disinfected before use, the water is treated by bleaching liquid, and then the residual bleaching liquid is removed by using sodium bicarbonate, so that no bleaching liquid residue exists in the water;
c. adding water into a water bucket for culturing the composite anaerobic bacteria, heating the water to 42 ℃, safely taking out a heating rod, and turning on an indoor heat source to keep the room temperature at 45 ℃;
d. c, putting the culture medium into the water heated in the step c, uniformly stirring for about 30min, and adding the prepared composite anaerobic bacteria;
e. and d, stirring the aqueous solution mixed with the strains and the culture medium in the step d, stirring for about 5min every 2h, allowing the strains to pass through a germination period of 8h, a germination period of 2h and a stabilization period of 4h, turning off an indoor heat source after lasting for 14h, opening a door in a greenhouse, cooling, and completing propagation of the composite anaerobic bacteria to obtain 1000 parts of composite anaerobic bacteria solution with the concentration of 11 ppm.
The fly and insect flying into the room should be avoided in the whole culture period.
The composite aerobic bacterium liquid comprises 0.2 part of aerobic bacteria, 24.5 parts of culture medium and 975.3 parts of water; the composite photosynthetic bacteria liquid comprises 1 part of photosynthetic bacteria, 34 parts of culture medium C and 965 parts of water. 1000 parts of composite aerobic bacteria and composite photosynthetic bacteria liquid are respectively obtained, and the concentration of the bacteria liquid is 11 ppm.
Adding strains into the river by using a strain distribution pipe device through a water pump, adjusting the water inlet depth of the strain distribution pipe according to the water depth, sequentially adding anaerobic bacteria, aerobic bacteria and photosynthetic bacteria strains into the river, and then uniformly stirring the strains;
example 2
The embodiment provides an underwater bacterium distribution process for eliminating black and odorous riverways.
The composite anaerobic strain is a mixed strain of 12% clostridium butyricum, 13% green thiobacillus, 18% lactobacillus acidophilus, 22% lactobacillus plantarum, 18% streptococcus faecalis and 17% pediococcus acidilactici;
the composite aerobic bacteria are mixed strains of 13 percent of denitrified thiobacillus, 18 percent of bacillus subtilis, 15 percent of bacillus licheniformis, 12 percent of bacillus coagulans, 18 percent of bacillus cereus and 24 percent of candida utilis;
the composite photosynthetic bacteria comprise mixed strains of 5 percent of rhodococcus sphaeroides, 89 percent of rhodopseudomonas palustris and 6 percent of rhodopseudomonas capsulata.
According to the example 1, 1000 parts of composite anaerobic bacteria, composite aerobic bacteria and composite photosynthetic bacteria are respectively cultured, the bacteria concentration is 11ppm, and the composite anaerobic bacteria, the composite aerobic bacteria and the composite photosynthetic bacteria are sequentially added into rivers.
Example 3
The embodiment provides an underwater bacterium distribution process for eliminating black and odorous riverways.
The composite anaerobic strain is a mixed strain of 18% clostridium butyricum, 16% green thiobacillus, 22% lactobacillus acidophilus, 16% lactobacillus plantarum, 13% streptococcus faecalis and 15% pediococcus acidilactici;
the composite aerobic bacteria are mixed strains of 17 percent of denitrified thiobacillus, 13 percent of bacillus subtilis, 18 percent of bacillus licheniformis, 15 percent of bacillus coagulans, 12 percent of bacillus cereus and 25 percent of candida utilis;
the composite photosynthetic bacteria comprise mixed strains of 10 percent of rhodococcus sphaeroides, 80 percent of rhodopseudomonas palustris and 10 percent of rhodopseudomonas capsulata.
According to the example 1, 1000 parts of composite anaerobic bacteria, composite aerobic bacteria and composite photosynthetic bacteria are respectively cultured, the bacteria concentration is 11ppm, and the composite anaerobic bacteria, the composite aerobic bacteria and the composite photosynthetic bacteria are sequentially added into rivers.
The data for detecting the change in river water quality in examples 1 to 3 are shown in Table 1.
TABLE 1
And (4) conclusion: according to experimental data, the average clearance rate of the invention on COD is 46.0%, the average clearance rate on ammonia nitrogen is 60.5%, the improvement rate on water turbidity is 51.5%, the content of escherichia coli in water is reduced by more than 83.3%, the total nitrogen content, reductive pollution and turbidity in river water are effectively reduced, the black and odorous river channel is removed, and the quality and appearance of the river water are improved.
Example 4
The embodiment provides an underwater bacterium distribution process for eliminating black and odorous riverways.
The strain distribution pipe device is formed by combining a plurality of stainless steel pipes with different diameters through a four-way joint, in two connected stainless steel pipes, a thick pipe port is provided with an internal thread and is in threaded connection with the four-way joint, and a thin pipe port is provided with an external thread and is in threaded connection with the four-way joint.
The bacterium distribution pipes are made of 304 stainless steel materials, the length of each stainless steel pipe is 0.5m, the stainless steel pipes are connected through four-way joints, and the lengths can be freely combined according to the depth of a river channel to adapt to the depths of different river channels.
The strain distribution pipe can be provided with branch pipes on the side of the square joint according to the pollution condition of the river channel and the strain feeding amount to form a loop. If the branch pipe is not installed, the side face of the square joint can be plugged by a plug.
The above description is only a preferred embodiment of the present invention, and not intended to limit the present invention, and the scope of the present invention is defined by the appended claims, and all changes that come within the meaning and range of equivalency of the specification are therefore intended to be embraced therein.
Claims (5)
1. An underwater bacterium distribution process for eliminating black and odorous riverways comprises the following steps:
(1) and (3) strain culture: respectively culturing composite anaerobic bacteria liquid, composite aerobic bacteria liquid and composite photosynthetic bacteria liquid, wherein the mass fraction of the cultured bacteria liquid is 1000 parts, and the concentration is 11 ppm;
the composite anaerobic strain is a mixed strain of 10-20% of clostridium butyricum, 10-20% of green thiobacillus, 15-25% of lactobacillus acidophilus, 15-25% of lactobacillus plantarum, 10-20% of streptococcus faecalis and 10-20% of pediococcus acidilactici;
the composite aerobic bacteria are mixed strains of 10-20% of thiobacillus denitrificans, 10-20% of bacillus subtilis, 10-20% of bacillus licheniformis, 10-20% of bacillus coagulans, 10-20% of bacillus cereus and 20-30% of candida utilis;
the composite photosynthetic bacteria comprise 5-10% of rhodococcus sphaeroides, 80-90% of rhodopseudomonas palustris and 5-10% of rhodopseudomonas capsulata.
(2) Adding in a river: the strain distribution pipe device is used for adding strains into the river through a water pump, the water inlet depth of the strain distribution pipe is adjusted according to the water depth, anaerobic bacteria, aerobic bacteria and photosynthetic bacteria strains are sequentially added into the river, and then the strains are uniformly stirred.
2. The underwater bacterium distribution process for eliminating the black and odorous river channel according to claim 1, which is characterized in that: the fungus tube distribution device in the step (2) is formed by combining a plurality of stainless steel tubes with different diameters through a four-way joint, and the stainless steel tubes are in threaded connection with the four-way joint.
3. The underwater bacterium distribution process for eliminating the black and odorous river channel according to claim 1, which is characterized in that:
the compound anaerobic bacteria liquid in the step (1) comprises 2 parts of anaerobic bacteria, 38 parts of culture medium A and 960 parts of water;
the composite aerobic bacterium liquid in the step (1) comprises 0.2 part of aerobic bacterium, 24.5 parts of culture medium B and 975.3 parts of water;
the composite photosynthetic bacteria liquid in the step (1) comprises 1 part of photosynthetic bacteria, 34 parts of culture medium C and 965 parts of water;
the specific bacteria liquid culture steps are as follows:
a. all the inner walls of the container, stirring rods, air bubble stones, heating rods and the like which can be contacted with the culture medium are disinfected by potassium permanganate, and potassium permanganate residues cannot exist after disinfection;
b. the water is disinfected before use, treated by bleaching solution, and then the residual bleaching solution is removed by using sodium bicarbonate, so that no bleaching solution residue is left in the water;
c. adding water into a water tank for culturing strains, heating the water to 36-42 ℃, safely taking out a heating rod, and turning on an indoor heat source to keep the room temperature at 45 ℃;
d. c, putting the culture medium into the water heated in the step c, uniformly stirring for about 30min, adding the prepared composite anaerobic bacteria, composite aerobic bacteria or composite photosynthetic bacteria, and separately culturing the three strains;
e. and d, stirring the aqueous solution mixed with the strains and the culture medium in the step d for about 5min every 2h, continuing for 14h, turning off an indoor heat source, opening a door in a greenhouse for cooling, and finishing the propagation.
4. The underwater bacterium distribution process for eliminating the black and odorous river channel according to claim 3, wherein the process comprises the following steps: the carbon source, the nitrogen source and the phosphorus source of the culture medium A are respectively sucrose, peptone, yeast and KH2PO4The optimum growth factor is tomato juice, and the optimum addition amount is determined by dumpling steaming experiment to be 2.0% of sucrose, 1.5% of peptone, 0.5% of yeast and KH2PO40.2% and tomato juice 5%.
5. The underwater bacterium distribution process for eliminating the black and odorous river channel according to claim 3, wherein the process comprises the following steps: the carbon source, the nitrogen source and the phosphorus source of the culture medium B are respectively corn flour, peptone and KNO3The best growth factor is beef extract, and the formula of the culture medium B is determined to be 7.5g/L corn flour and 15g/L, KNO g/peptone by a two-level Plackett-Burman test31g/L beef extract 7.5g/L, NaCl 5g/L, CaCO g30.75g/L、ZnSO40.7g/L and H2O 0.0075g/L。
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