CN110357715A - A kind of liquid bacterial agent formula of alternative solid biofertilizer and preparation method thereof - Google Patents
A kind of liquid bacterial agent formula of alternative solid biofertilizer and preparation method thereof Download PDFInfo
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- CN110357715A CN110357715A CN201910696201.2A CN201910696201A CN110357715A CN 110357715 A CN110357715 A CN 110357715A CN 201910696201 A CN201910696201 A CN 201910696201A CN 110357715 A CN110357715 A CN 110357715A
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- bacterial agent
- liquid bacterial
- agent formula
- alternative solid
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Abstract
The invention discloses liquid bacterial agent formula of a kind of alternative solid biofertilizer and preparation method thereof, a kind of Contents of Main Components of the liquid bacterial agent formula of alternative solid biofertilizer: brewer's yeast 0.5%, lactobacillus plantarum 1%, lactobacillus acidophilus 1%, 1-10 parts of potato, 2-20 parts of brown sugar, 2-10 parts of glucose, 0.2-4 parts of peptone, 0.02-0.4 parts of magnesium sulfate, 0.02-0.4 parts of manganese sulfate, 0.02-2 parts of dipotassium hydrogen phosphate, 0.2-4 parts of sodium chloride, 70-98 parts of water.The present invention is using brewer's yeast, lactobacillus plantarum and lactobacillus acidophilus as bacterial strain, inoculation conical flask prepares liquid spawn respectively, potato, brown sugar, glucose, peptone, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, sodium chloride are mixed, add clean water dissolution, heat treatment method sterilizes, as culture medium, manufacture the higher liquid bacterial agent of utilization efficiency, chemical fertilizer utilization efficiency is improved, fertilizer application amount is reduced, promotes Agricultural product without pollution.
Description
Technical field
The present invention relates to the liquid bacterial agent formula of liquid bacterial agent field more particularly to a kind of alternative solid biofertilizer and
Preparation method.
Background technique
A kind of liquid bacterial agent formula of alternative solid biofertilizer is with brewer's yeast, lactobacillus plantarum and acidophilus cream bar
Bacterium is primary raw material, makes the liquid bacterial agent of efficient alternative solid biofertilizer, improves chemical fertilizer utilization efficiency, and reducing chemical fertilizer makes
Dosage promotes Agricultural product without pollution.
Summary of the invention
The object of the present invention is to provide liquid bacterial agent formula of a kind of alternative solid biofertilizer and preparation method thereof, energy
The solid biofertilizer of enough substitutions at this stage, manufactures the higher liquid bacterial agent of utilization efficiency, improves chemical fertilizer utilization efficiency, reductionization
Fertile usage amount promotes Agricultural product without pollution.
In order to achieve the above object, the present invention is achieved by the following technical solutions:
The present invention provides a kind of liquid bacterial agent formula and preparation method thereof of alternative solid biofertilizer, which can replace
For the Contents of Main Components of the liquid bacterial agent formula of solid biofertilizer: brewer's yeast 0.5%, lactobacillus plantarum 1%, acidophilus cream
Bacillus 1%, 1-10 parts of potato, 2-20 parts of brown sugar, 2-10 parts of glucose, 0.2-4 parts of peptone, 0.02-0.4 parts of magnesium sulfate, sulphur
Sour manganese 0.02-0.4 parts, 0.02-2 parts of dipotassium hydrogen phosphate, 0.2-4 parts of sodium chloride, 70-98 parts of water.
Preferably, a kind of Contents of Main Components of the liquid bacterial agent formula of alternative solid biofertilizer: brewer's yeast
0.5%, lactobacillus plantarum 1%, lactobacillus acidophilus 1%, 1 part of potato, 2 parts of brown sugar, 2 parts of glucose, 0.2 part of peptone, sulfuric acid
0.02 part of magnesium, 0.02 part of manganese sulfate, 0.02 part of dipotassium hydrogen phosphate, 0.2 part of sodium chloride, 70 parts of water.
Preferably, a kind of Contents of Main Components of the liquid bacterial agent formula of alternative solid biofertilizer: brewer's yeast
0.5%, lactobacillus plantarum 1%, lactobacillus acidophilus 1%, 5 parts of potato, 10 parts of brown sugar, 6 parts of glucose, 2 parts of peptone, magnesium sulfate
0.2 part, 0.2 part of manganese sulfate, 0.1 part of dipotassium hydrogen phosphate, 0.2 part of sodium chloride, 84 parts of water.
Preferably, a kind of Contents of Main Components of the liquid bacterial agent formula of alternative solid biofertilizer: brewer's yeast
0.5%, lactobacillus plantarum 1%, lactobacillus acidophilus 1%, 10 parts of potato, 20 parts of brown sugar, 10 parts of glucose, 4 parts of peptone, sulfuric acid
0.4 part of magnesium, 0.4 part of manganese sulfate, 2 parts of dipotassium hydrogen phosphate, 4 parts of sodium chloride, 98 parts of water.
Preferably, the shape of a kind of liquid bacterial agent formula of alternative solid biofertilizer and preparation method thereof brewer's yeast
For subsphaeroidal ellipsoid, color is faint yellow, no bitter taste, and taste is general.
Preferably, a kind of liquid bacterial agent formula of alternative solid biofertilizer and preparation method thereof lactobacillus plantarum is circle
Straight-bar bacterium is held, usually 0.9~1.2vtm × 3.0~8.0 μm, single, pairs of or short chain, Gram-positive, spore of not sprouting,
Amphimicrobian, surface colony diameter about 3mm, protrusion, rounded, surface is smooth, and fine and closely woven, color is white, is once in a while in pale yellow or buff.
Preferably, a kind of liquid bacterial agent formula of alternative solid biofertilizer and preparation method thereof lactobacillus acidophilus is cream
The end of Bacillus, Gram-positive bacillus, bar is rounded.
The present invention also provides a kind of preparation method of the liquid bacterial agent formula of alternative solid biofertilizer, specifically include with
Lower step:
(1) a kind of liquid bacterial agent formula of alternative solid biofertilizer described in -4 any one according to claim 1
And preparation method thereof Contents of Main Components prepare raw material；
(2) potato, brown sugar, glucose, peptone, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, sodium chloride are mixed, is added clean
Net water dissolution, heat treatment method sterilizes, as culture medium；
(3) using brewer's yeast, lactobacillus plantarum and lactobacillus acidophilus as bacterial strain, it is inoculated with conical flask respectively and prepares liquid
Strain；
(4) liquid spawn of brewer's yeast is first inoculated in above-mentioned culture medium, 28-32 DEG C is cultivated 16~24 hours, will be planted
Then mixed liquor is seeded in culture medium by object lactobacillus and lactobacillus acidophilus again by 1: 1 volume ratio production mixed liquor,
34-37 DEG C is continued culture 24~30 hours, and pH value is down to 3.5-4.5.
(5) step 4 product is aseptic subpackaged, liquid bacterial agent is made.
Beneficial effects of the present invention: the present invention can substitute solid biofertilizer at this stage, and manufacture utilization efficiency is higher
Liquid bacterial agent, improve chemical fertilizer utilization efficiency, reduce fertilizer application amount, promote Agricultural product without pollution.
Specific embodiment
With reference to embodiment, the specific embodiment of the present invention is further described.Following embodiment is only used for more
Add and clearly demonstrate technical solution of the present invention, and not intended to limit the protection scope of the present invention.
Embodiment 1
Specific step is as follows for production method:
(1) prepare according to Contents of Main Components: brewer's yeast 0.5%, lactobacillus plantarum 1%, lactobacillus acidophilus 1%, soil
1 part of beans, 2 parts of brown sugar, 2 parts of glucose, 0.2 part of peptone, 0.02 part of magnesium sulfate, 0.02 part of manganese sulfate, dipotassium hydrogen phosphate 0.02
Part, 0.2 part of sodium chloride, 70 parts of water；
(2) potato, brown sugar, glucose, peptone, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, sodium chloride are mixed, is added clean
Net water dissolution, heat treatment method sterilizes, as culture medium；
(3) using brewer's yeast, lactobacillus plantarum and lactobacillus acidophilus as bacterial strain, it is inoculated with conical flask respectively and prepares liquid
Strain；
(4) liquid spawn of brewer's yeast is first inoculated in above-mentioned culture medium, 28-32 DEG C is cultivated 16~24 hours, will be planted
Then mixed liquor is seeded in culture medium by object lactobacillus and lactobacillus acidophilus again by 1: 1 volume ratio production mixed liquor,
34-37 DEG C is continued culture 24~30 hours, and pH value is down to 3.5-4.5.
(5) step 4 product is aseptic subpackaged, liquid bacterial agent is made.
Embodiment 2
Specific step is as follows for production method:
(1) prepare according to Contents of Main Components: brewer's yeast 0.5%, lactobacillus plantarum 1%, lactobacillus acidophilus 1%, soil
5 parts of beans, 10 parts of brown sugar, 6 parts of glucose, 2 parts of peptone, 0.2 part of magnesium sulfate, 0.2 part of manganese sulfate, 0.1 part of dipotassium hydrogen phosphate, chlorine
Change 0.2 part of sodium, 84 parts of water；
(2) potato, brown sugar, glucose, peptone, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, sodium chloride are mixed, is added clean
Net water dissolution, heat treatment method sterilizes, as culture medium；
(3) using brewer's yeast, lactobacillus plantarum and lactobacillus acidophilus as bacterial strain, it is inoculated with conical flask respectively and prepares liquid
Strain；
(4) liquid spawn of brewer's yeast is first inoculated in above-mentioned culture medium, 28-32 DEG C is cultivated 16~24 hours, will be planted
Then mixed liquor is seeded in culture medium by object lactobacillus and lactobacillus acidophilus again by 1: 1 volume ratio production mixed liquor,
34-37 DEG C is continued culture 24~30 hours, and pH value is down to 3.5-4.5.
(5) step 4 product is aseptic subpackaged, liquid bacterial agent is made.
Embodiment 3
Specific step is as follows for production method:
(1) prepare according to Contents of Main Components: brewer's yeast 0.5%, lactobacillus plantarum 1%, lactobacillus acidophilus 1%, soil
10 parts of beans, 20 parts of brown sugar, 10 parts of glucose, 4 parts of peptone, 0.4 part of magnesium sulfate, 0.4 part of manganese sulfate, 2 parts of dipotassium hydrogen phosphate, chlorine
Change 4 parts of sodium, 98 parts of water；
(2) potato, brown sugar, glucose, peptone, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, sodium chloride are mixed, is added clean
Net water dissolution, heat treatment method sterilizes, as culture medium；
(3) using brewer's yeast, lactobacillus plantarum and lactobacillus acidophilus as bacterial strain, it is inoculated with conical flask respectively and prepares liquid
Strain；
(4) liquid spawn of brewer's yeast is first inoculated in above-mentioned culture medium, 28-32 DEG C is cultivated 16~24 hours, will be planted
Then mixed liquor is seeded in culture medium by object lactobacillus and lactobacillus acidophilus again by 1: 1 volume ratio production mixed liquor,
34-37 DEG C is continued culture 24~30 hours, and pH value is down to 3.5-4.5.
(5) step 4 product is aseptic subpackaged, liquid bacterial agent is made.
The foregoing is only a preferred embodiment of the present invention, but scope of protection of the present invention is not limited thereto,
Anyone skilled in the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (8)
1. a kind of liquid bacterial agent formula of alternative solid biofertilizer and preparation method thereof, which is characterized in that the one kind can be replaced
For the Contents of Main Components of the liquid bacterial agent formula of solid biofertilizer: brewer's yeast 0.5%, lactobacillus plantarum 1%, acidophilus cream
Bacillus 1%, 1-10 parts of potato, 2-20 parts of brown sugar, 2-10 parts of glucose, 0.2-4 parts of peptone, 0.02-0.4 parts of magnesium sulfate, sulphur
Sour manganese 0.02-0.4 parts, 0.02-2 parts of dipotassium hydrogen phosphate, 0.2-4 parts of sodium chloride, 70-98 parts of water.
2. a kind of liquid bacterial agent formula and preparation method thereof of alternative solid biofertilizer according to claim 1,
It is characterized in that, a kind of Contents of Main Components of the liquid bacterial agent formula of alternative solid biofertilizer: brewer's yeast 0.5%,
Lactobacillus plantarum 1%, lactobacillus acidophilus 1%, 1 part of potato, 2 parts of brown sugar, 2 parts of glucose, 0.2 part of peptone, magnesium sulfate 0.02
Part, 0.02 part of manganese sulfate, 0.02 part of dipotassium hydrogen phosphate, 0.2 part of sodium chloride, 70 parts of water.
3. a kind of liquid bacterial agent formula and preparation method thereof of alternative solid biofertilizer according to claim 1,
It is characterized in that, a kind of Contents of Main Components of the liquid bacterial agent formula of alternative solid biofertilizer: brewer's yeast 0.5%,
Lactobacillus plantarum 1%, lactobacillus acidophilus 1%, 5 parts of potato, 10 parts of brown sugar, 6 parts of glucose, 2 parts of peptone, 0.2 part of magnesium sulfate,
0.2 part of manganese sulfate, 0.1 part of dipotassium hydrogen phosphate, 0.2 part of sodium chloride, 84 parts of water.
4. a kind of liquid bacterial agent formula and preparation method thereof of alternative solid biofertilizer according to claim 1,
It is characterized in that, a kind of Contents of Main Components of the liquid bacterial agent formula of alternative solid biofertilizer: brewer's yeast 0.5%,
Lactobacillus plantarum 1%, lactobacillus acidophilus 1%, 10 parts of potato, 20 parts of brown sugar, 10 parts of glucose, 4 parts of peptone, magnesium sulfate 0.4
Part, 0.4 part of manganese sulfate, 2 parts of dipotassium hydrogen phosphate, 4 parts of sodium chloride, 98 parts of water.
5. a kind of liquid bacterial agent formula and preparation method thereof of alternative solid biofertilizer according to claim 1,
It is characterized in that, the shape of the brewer's yeast is subsphaeroidal ellipsoid, and color is faint yellow, no bitter taste, and taste is general.
6. a kind of liquid bacterial agent formula and preparation method thereof of alternative solid biofertilizer according to claim 1,
Be characterized in that, the lactobacillus plantarum is nose circle straight-bar bacterium, usually 0.9~1.2vtm × 3.0~8.0 μm, it is single, in pairs or
Short chain, Gram-positive, spore of not sprouting, amphimicrobian, surface colony diameter about 3mm, protrusion, rounded, surface is smooth, carefully
Close, color is white, is once in a while in pale yellow or buff.
7. a kind of liquid bacterial agent formula and preparation method thereof of alternative solid biofertilizer according to claim 1,
It is characterized in that, the lactobacillus acidophilus is lactobacillus, and the end of Gram-positive bacillus, bar is rounded.
8. a kind of preparation side of the liquid bacterial agent formula of alternative solid biofertilizer according to claim 1-7
Method, which is characterized in that specifically includes the following steps:
(1) according to claim 1 a kind of liquid bacterial agent formula of alternative solid biofertilizer described in -4 any one and its
The Contents of Main Components of preparation method prepares raw material；
(2) potato, brown sugar, glucose, peptone, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate, sodium chloride are mixed, adds cleaning
Water dissolution, heat treatment method sterilizes, as culture medium；
(3) using brewer's yeast, lactobacillus plantarum and lactobacillus acidophilus as bacterial strain, it is inoculated with conical flask respectively and prepares liquid bacteria
Kind；
(4) liquid spawn of brewer's yeast is first inoculated in above-mentioned culture medium, 28-32 DEG C is cultivated 16~24 hours, by plant cream
Then mixed liquor is seeded in culture medium, by 1: 1 volume ratio production mixed liquor in 34-37 by bacillus and lactobacillus acidophilus again
DEG C continue culture 24~30 hours, pH value is down to 3.5-4.5.
(5) step 4 product is aseptic subpackaged, liquid bacterial agent is made.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910696201.2A CN110357715A (en) | 2019-07-30 | 2019-07-30 | A kind of liquid bacterial agent formula of alternative solid biofertilizer and preparation method thereof |
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| CN109251874A (en) * | 2018-09-25 | 2019-01-22 | 厦门惠盈动物科技有限公司 | A kind of probiotics preparation and its preparation method and application |
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2019
- 2019-07-30 CN CN201910696201.2A patent/CN110357715A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1911870A (en) * | 2006-09-01 | 2007-02-14 | 山东省农业科学院高新技术研究中心 | Liquid composite microorganism fertilizer and its preparation method |
| CN101690545A (en) * | 2009-09-07 | 2010-04-07 | 肇东市日成酶制剂有限公司 | Method for producing complex micro-ecological preparation with microbial agents and enzyme |
| CN102898188A (en) * | 2012-08-27 | 2013-01-30 | 徐珊 | Concentrated liquid fertilizer and preparation method thereof |
| CN106047770A (en) * | 2016-08-08 | 2016-10-26 | 江苏春语生物科技有限公司 | Composite liquid microbial agent with long quality guarantee period and preparation method thereof |
| CN206570318U (en) * | 2017-02-16 | 2017-10-20 | 潍坊昆腾农业发展股份有限公司 | Bio-bacterial manure strain process units |
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Application publication date: 20191022 |
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