KR101925635B1 - The mixture for cultivation of fragrant mushroom for increased cell growth speed - Google Patents

Abstract

The present invention relates to a new mixed composition used for cultivating shiitake mushroom, which stimulates Crista in mitochondria of shiitake mushroom to promote cell metabolism, thereby enhancing the cell division rate of mushroom cells, To a mixed composition.
The mixed composition for improving the growth rate through the shiitake mushroom crystal stimulation according to the present invention can enhance the cell division rate of mushroom by activating Crista in mitochondria. As a result, the growth rate of shiitake mushroom can be increased.
The mixed composition for improving the growth rate through the shiitake mushroom stimulation according to the present invention is characterized by being composed of magnesium stearate, potassium sorbate, magnesium carbonate, sodium alginate and methyl cellulose.

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for improving growth speed through stimulation of shiitake mushroom,

The present invention relates to a mixed composition for use in a method for cultivating shiitake mushroom. More particularly, the present invention relates to a mixed composition for promoting cell metabolism by stimulating Crista in mitochondria of shiitake mushroom to improve cell division rate of mushroom cells, To a mixed composition capable of improving the growth rate.

Lentinus edodes is an edible mushroom belonging to the genus Bacillus subtilis and pine mushroom in the spring and autumn. It is a wood white rot fungus which grows on the stump and grows on the stump. Korea, China, Japan, Southeast Asia , And New Zealand (Kim et al., 2003). Shiitake mushrooms contain 81.8 ~ 90% of water, and it contains protein, amino acids, fat, ash, minerals, vitamins and dietary fiber. Dietary fiber contains fibrous components and various polysaccharides on the cell walls of mushrooms (Bartnicki-Garcia, 1968). The physiological characteristics of shiitake mushrooms include anticancer effect, antimicrobial activity, hepatitis B treatment effect, and antiviral studies. Among the studies, lentinan, a β-1,3 glucan isolated from mushroom fruiting body, has not been shown to have anticancer effects directly, but it has been reported that it enhances immune function by participating in host mediated immune response (Chihara , 1992).

The natural distribution of shiitake mushrooms is on the Korean Peninsula, Japan, China, Taiwan, the Philippines, Cerebes, New Guinea and Nepal. The natural shiitake mushroom is grown in the natural forest with the spore of the shiitake mushroom attached to the wind or the dead tree.

In the past, we took advantage of this point and used a primitive cultivation method that leaves an ax mark on the wood slice and waits for the seed to grow with it. After that, the mushroom cultivated by artificially cultivating mushroom fungus and then inoculating it into the logs has developed into a highland logging method, and the productivity of the elevation has been greatly improved.

Particularly, oak has a high density and is made of a hard material, and a method of cultivating oak mushroom using the characteristics of such oak has been developed. That is, a method of cultivating shiitake mushroom has already been developed by utilizing the method of the noi cultivation method, the method of cultivating the facility house, and the sawdust cultivation method.

However, the conventional method of cultivating shiitake mushrooms is only a method for cultivating shiitake mushrooms, and studies or attempts to improve the growth rate by stimulating Crista in mitochondria of shiitake mushrooms have not been made.

Mitochondria, one of the cell organelles, are involved in cellular respiration. Thus, the more active the respiratory cells, the more mitochondria they contain and the energy-producing plant is called. In 1897, Carl Benda proved the presence of mitochondria in the cell, and that the shape of the cell was in the form of a collagen and a spring, and was present in almost all cytoplasm. The name "Mitochondria" was used as a result of observing the shape of the figure. The word "Mitos" meaning Greek thread and "Chondrin" meaning granule or particle were synthesized To name it. Before Karl Bender was able to prove the existence of mitochondria, it was known by many scientists. In 1886, German biologist Richard Altmann called mitochondria a bio-blast, I had first grasped the importance. In addition, Mitochondria were called by various names such as Condriosomes, Chromidia, Condrio Cont, but their existence was not accepted in the scientific community. Mitochondria are surrounded by a double membrane, and the inside is composed of a twisty inner membrane called Cristae. There are DNA and RNA in it, and it is about 0.2 ~ 3㎛ in size and is involved in cell respiration. The shape varies according to species, and the size varies depending on the type of cell, but it is often about 0.5 ㎛ wide and 2 ㎛ long. Using a phase contrast microscope, it can be observed in living cells, stained with Janus Green B and easily distinguished from other parts. It is also detected by Nadir reaction against cytochrome oxidase and tetrazolium salt reduction reaction with succinic acid dehydrogenase. The number of mitochondria contained in one cell is related to the energy acceptance of the cell, and generally, the more active the respiration, the more mitochondria it contains. For example, 1,000 to 3,000 cells per hepatocyte, and 100 to 200 mitochondria in plant cells. It is known that there are about 1 mole of mitochondria in the human body. There are about 100,000 eggs in female eggs and about 100 in male sperm. When observing mitochondria under an electron microscope, a very complex structure can be seen. It has a double structure of outer membrane and inner membrane, and the inner membrane is like a folded labyrinth. On the inside of the inner membrane is DNA (deoxyribonucleic acid) that carries genetic information. This inner lobes are called crista. Christa is often arranged perpendicular to the long axis of mitochondria. Some cells are arranged parallel or irregularly. Biologists may also consider it to be an independent bacterium because of its biomolecule and unique DNA properties. In the beginning, it is likely that he acted as an independent germ.

There are many enzymes in the mitochondria.

① Enzyme present in the outer membrane: monoamine oxidase, fatty acid thiokinase, kinurinin hydroxylase, cytochrome C reductase.

② Enzyme present between inner and outer membrane: adenylate kinase · nucleotide diphosphate kinase

③ Enzyme present in inner membrane: keto acid dehydrogenase · succinate dehydrogenase · α- β-oxybutyrate dehydrogenase · Carnitine-acyl transferase · Terminal electron transport system ATP synthase

④ Enzymes present in stroma: citric acid synthase, isocitrate dehydrogenase, fumarase, malic acid dehydrogenase, aconitase, glutamate dehydrogenase, β-oxidase system of fatty acid.

The most important function of mitochondria is to synthesize ATP, an energy source, through food in the body. In the inner membrane of mitochondria, there is a protein called 'ATP synthase', which plays a role in producing ATP. The hydrogen ions formed between the inner membrane and the outer membrane of mitochondria are introduced into the inner mitochondrial membrane through the food, and the ATP synthase binds the phosphoric acid and ADP (form of two phosphoric acid and adenosine) to form ATP (three phosphoric acid and adenosine Combined form) is made. The American chemist Paul Boyer has discovered the ability of this mitochondrial ATP synthesis.

Mitochondria also play a role in killing lost cells. This is called apoptosis, which causes the DNA to absorb the destroyed cells or intercept DNA to lead to death. This is a role that prevents cells that have already lost function from being transformed into cancer cells or other cells.

Mitcondria is a structure that plays a central role in controlling respiration. It is also involved in the oxidation of pyruvic acid, acetyl-CoA, produced by the catabolism of lipids, and oxidative phosphorylation through the electron transport system do. Thus, mitochondria play a central role in the oxidation of sugars and fatty acids and the accompanying oxidative phosphorylation.

In addition, various amino acids accumulate nitrogen as an α-amino group of glutamic acid through an amino group transfer mechanism. After making this nitrogen into ammonia by the action of glutamate dehydrogenase, there is enzyme activity of urea synthesis system which converts it into urea. In mitochondria, a part of the reaction of transferring acetyl-CoA to a fatty acid to extend the fatty acid chain is also performed. The function of the mitochondrion is sensitive to its structural changes. For example, mitochondria swollen with thyroxine respiratory, but not with oxidative phosphorylation.

As the muscles in the body grow, more mitochondria are produced in more muscle cells, resulting in more energy, ATP. Thus, physical strength is increased and endurance is strengthened. However, if you do not exercise constantly, the mitochondria will disappear or the volume will decrease, so the energy of the human body will decrease. However, mitochondria produce active oxygen during the production of ATP, which can destroy proteins or adversely affect DNA. Cells have the ability to defend against free radicals, but their role is weakened as they get older and become cancerous or diabetes, hypertension and heart disease.

Mitochondria are self-proliferating, and they can multiply themselves. It has been confirmed that DNA inherent in the mitochondria exists and that a unique protein synthesis system exists. The DNA or protein synthesis system of mitochondria is similar to that of bacteria. This suggests that cardiolipin (a type of phospholipid) present in the cytoplasm of bacteria is also localized in mitochondria, and that mitochondria have a similar origin to bacteria. Mutations in the genes specific to mitochondria are also known.

The mitochondria in the mushroom cells are as follows. Within the shiitake mushrooms, round circular mitochondria grow to about 50 μm and are present in several. The mitochondria of shiitake mushrooms are wrapped in two layers of mitochondria inside and outside. The film thickness is about 6 nm, the film thickness is 6 to 8 nm, and the inner film protrudes inward and forms a fist-shaped crista with a width of 20 to 30 μm. Mitochondria have a group of enzymes for oxidative phosphorylation that catalyzes the citric acid cycle, the electron transport system, and both, and becomes a field of energy production under aerobic conditions. The substrate contains an enzyme that produces acetyl CoA in pyruvic acid or fatty acid, or an enzyme that oxidizes acetyl CoA in a citric acid circuit. The final products of this oxidation process are CO2 and NADH, CO2 is released from the cell, and NADH is the main source of electrons flowing through the respiratory chain. The crystals and the inner membrane are buried with various enzymes involved in electron transfer and ATPase involved in oxidative phosphorylation which is conjugated to them. The basic particles contain a membrane-penetrating proton carrier as part of the ATPase and synthesize ATP when the proton passes through it by an electrochemical gradient.

Patent Registration No. 10-1174245 (Registration date 2012.08.08) Japanese Patent Application Laid-Open No. 10-2014-0021857 (published on Feb. 21, 2014)

In order to solve the above-mentioned problems, the present invention provides a mixed composition capable of stimulating mitochondrial Crista in shiitake mushroom cells to promote cell division of mushroom and thereby improving the growth rate of mushroom It has its main purpose.

Other objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.

In order to achieve the above objects, the present invention provides a mixed composition for enhancing the growth rate through a shiitake mushroom crystal stimulation, comprising 9.8 to 10.2 wt% of magnesium stearate, 29.5 to 30.5 wt% of potassium sorbate, , 29.5 to 30.5 wt% of magnesium carbonate, 14.8 to 15.2 wt% of sodium alginate, and 14.8 to 15.2 wt% of methyl cellulose.

In this case, the mixed composition was coated on the prepared silicon paper to a thickness of 0.1 mm or less, and the silicon paper coated with the mixed composition had a wavelength of 1038 nm to 1050 nm, a frequency of 4.5 to 4.8 Hz, an average output optical power of 55 To 65 W of laser for 10 minutes, wherein the silicon paper is prepared by cutting a single crystal silicon having a purity of 99.9999999% in a thin state to smoothly treat the surface, without any defect or contamination on the surface, It has a flatness of 0.2 mm and a diameter of 20 cm.

On the other hand, the magnesium stearate has an average particle size of 20 nm, the potassium sorbate has an average particle size of 40 nm, the magnesium carbonate has an average particle size of 80 nm, The average size of the sodium alginate particles is 80 nm, and the average size of the methyl cellulose particles is 80 nm.

According to another aspect of the present invention, there is provided a method of cultivating a mushroom of the present invention comprising the steps of: (A) preparing an oak seedlings having a skin; (B) forming a seedling hole in the wood, (C) a step of laying the log (alley) into which the shiitake mushroom has been introduced under the condition of 60% to 70% of shade and 40% to 30% of sunshine, (D) stepping up the alley in the fall season, which is between November and December or the following February, and (D) (E) dissolving a special mixed composition in water to produce a liquid for spraying mushroom; and (F) repeatedly spraying the generated mushroom spraying liquid onto the alley at intervals of 15 days before harvesting It is.

Preferably, the step (A) comprises: drying oak seedlings having epidermis having a diameter of 10 to 20 cm from November to February; drying the oak seedlings from the shade; and drying the dried oak timber to a length of 1 to 1.2 m And the step of cutting is carried out to the inoculation site.

Preferably, the step (C) is a step of keeping the wood (alley) into which the shiitake mushroom has been introduced in a place where ventilation and drainage are well, keeping the humidity at 70 to 80%, keeping the temperature at 20 to 28 ° C, A step of laying down a tree (alley) into which the shiitake mushroom seedlings have been laid down by one month at a time, .

Preferably, the step (D) is a step of spraying lime or veno ram wettable powder before the alley is set up, and a step of setting the distance between the logs to be about 15 cm The method comprising the steps of:

Preferably, the constituent material for improving the growth rate of the mushroom in the step (E) is magnesium stearate, potassium sorbate, magnesium carbonate, sodium alginate, and methyl cellulose.

Preferably, the water content of the mixed composition solution for improving the growth rate of the shiitake mushroom sprayed in the step (F) is about 15 seconds per one alley.

The mixed composition for improving the growth rate through the shiitake cristal stimulation according to various embodiments of the present invention can enhance the cell division rate of mushroom by activating Crista in mitochondria. As a result, the growth rate of shiitake mushroom can be increased.

Hereinafter, various embodiments of the present invention will be described in detail. However, the present invention is not limited or limited by the embodiments. Also, in various embodiments of the present invention, the composition ratios of the materials can be varied in design.

The composition of the mixed composition for improving the growth rate through the shiitake mushroom crystal stimulation according to the present invention will be described below.

< Magnesium stearate >

Magnesium stearate is a food additive that improves the stickiness and viscosity of food, improves emulsion stability, and improves the physical properties and texture of food. Magnesium stearate is used in foods as anti-caking agents, binders, emulsifiers, thickeners, and the like. Magnesium stearate is a white light powder with a slight peculiar odor. Magnesium stearate is a mixture of magnesium salts of stearic acid and palmitic acid and is insoluble in water, alcohols, ethers and the like. The daily allowable daily intake (ADI) is not established due to lack of safety or lack of research. The use standard of magnesium stearate should be 1% or less in the food. It is mainly used as anti-caking agent, binder, emulsifier, thickener, etc. in food.

<Potassium sorbate>

Potassium sorbate is a food additive used as a preservative for processed foods by inhibiting the growth of microorganisms. Potassium sorbate is used in cheese, processed meat products, soups and the like. Potassium sorbate is a white or light yellow crystalline powder with no odor or a slight odor. The formula of potassium sorbate is C6H7O2K and its melting point is 270 ℃. Sorbic acid salts have potassium, sodium and calcium salts, but potassium type is used for food because it is high in solubility, safe, and easy to manufacture. Antibacterial activity is about 74% of sorbic acid, but it dissolves well in water. It is slightly soluble in propylene glycol (5.0%) and insoluble in acetone and ether (0.1% or less). If left in air, it is oxidized and colored. It inhibits the germination and growth of microorganism spores and acts to inhibit the growth of microorganisms. It acts mainly on yeast and mold, and bacteria act selectively. The lower the pH, the higher the solubility of ingredients such as sugar and salt, the better the antibacterial effect. Cheese, processed meat products, doenjang, kochujang, red bean, dried bean curd, dried fruit, tomato ketchup, fermented beverage, fruit wine, margarine, processed sugar products. The acceptable daily intake (ADI) is 0.0 to 25.0 mg / kg bw. The LD50 (Eld 50) for mouse (experimental mouse) is 5.86 g / kg (oral) and 1.28 g / kg (subcutaneous). The degree of toxicity is not severe and no signs of carcinogenesis are present, but hyperplasia occurs when overdosed. However, when used in combination with nitrite, which is a type of coloring agent, the antimicrobial effect is increased, but DNA damaging substances are generated, which causes cancer, or causes central nerve paralysis, hemorrhagic gastritis, liver adverse effects, chromosomal abnormalities, . When used in combination with nitrite, it is safe to use in foods with a pH of 6.0 or higher.

<Magnesium carbonate>

Magnesium carbonate is a magnesium salt of carbonic acid, which is naturally produced as magneite, colorless crystals or powder. Anhydrides (anhydrides) and monohydrates, trihydrates, and pentahydrates are known. It is industrially produced and used as a raw material for producing a magnesium compound, a vulcanized rubber admixture and the like.

The formula of magnesium carbonate is MgCO3. It is naturally produced as a magnesite. In addition, dolomite is MgCO3 · CaCO3 with calcium carbonate. Colorless crystals or powder with a specific gravity of 3.073. Water slightly soluble in water and carbon dioxide CO2 is dissolved in magnesium bicarbonate Mg (HCO3) 2. MgCO 3 + CO 2 + H 2 O → Mg (HCO 3) 2 When magnesium bicarbonate is produced, a portion of magnesium carbonate hydrolyzes to form magnesium hydroxide, forming a basic magnesium carbonate having a composition of 4MgCO 3 · Mg (OH) 2 · 4H 2 O as a whole. It dissolves in acid and does not dissolve in acetone and ammonia. Anhydrides (anhydrides) and monohydrates, trihydrates, and pentahydrates are known.

MgCO3 = MgO + CO2 In the laboratory, sodium carbonate is added to the magnesium salt to form a precipitate. A method of using magnesium salt in seawater industrially, a method of making trihydrate using ammonia method and drying it in an air stream of carbon dioxide, and the like. In addition, carbon dioxide may be blown into the suspension (suspension) of magnesium hydroxide, calcined dolomite solids (suspension). It is used as a raw material for the production of magnesium compounds, and is used as vulcanization rubber admixture, toothpaste, antacid, insulating agent, abrasive.

< Sodium alginate >

Sodium Alginate is a food additive that improves the stickiness and viscosity of food, improves emulsion stability, and improves the physical properties and texture of food. A stabilizer for ice cream, an adhesive such as ketchup, mayonnaise and sauce, a refining agent for cheongju, a gelling agent, an emulsifier, a thickener and the like.

The formula for sodium alginate is (C6H7O6Na) n. White to pale yellowish fibrous, granular, granular or powdery with almost no odor and no taste. It does not dissolve in alcohol and organic solvent such as chloroform does not dissolve but slowly dissolves in sodium carbonate, sodium hydroxide, sodium phosphate. It is viscous when dissolved in water, and its effect is excellent as a stabilizer and thickener. The pH of the 1% aqueous solution is 6-8. The viscosity can be lowered when the aqueous solution is left at a high temperature for a long period of time.

Further, when a metal ion having two or more valences such as calcium chloride, barium chloride, aluminum sulfate, zinc sulfate, copper sulfate, silver nitrate, iron (III) chloride and lead acetate is added to the aqueous solution, However, the water-soluble magnesium salt and mercury salt do not solidify, and in the salt of 8% or more, they solidify and lose viscosity.

Therefore, when making an aqueous solution, it is advisable to mix it with alcohol or propylene glycol and then add water to dissolve. When acid is added to the aqueous solution of sodium alginate, alginic acid precipitates and precipitates. It begins to melt at pH 5.8 and becomes viscous liquid. The viscosity varies depending on the concentration, temperature, degree of polymerization, etc., and is 70 cps when the polymerization degree is 75, 140 cps when the polymerization degree is 84, and 1,000 cps when the polymerization degree is 130.

Dietary allowance (ADI) of the FAO / WHO Expert Committee is not established, but diarrhea symptoms have been reported when high concentrations of alginate, such as alginic acid, ammonium alginate, calcium alginate, potassium alginate and sodium alginate, are ingested. LD50 (eld 50) is 200 mg / kg when intravenously injected with sodium alginate and LD50 is 5000 mg / kg or more when administered orally to rats.

When added to ice cream as an ice cream stabilizer, it works with Ca2 + of milk to stabilize the texture of calcium alginate by forming a metal salt, which makes the ice crystals finer, smoothening the tissue, and maintaining the viscosity and shape. This is because water absorbed by sodium alginate as a hydrophilic polymer is hard to form ice crystals due to its strong hydration ability.

It is also used as an adhesive and thickening agent for ketchup, mayonnaise and sauce, and as a refining agent for sake. It is also used to prevent aging of noodles, stabilized orange and beer foam stabilizers, meat coatings, gels and jelly gels. It is hygroscopic and should be stored in a sealed container when stored.

<Methylcellulose>

Methyl cellulose (methyl cellulose) is used for improving the preservation and regeneration of frozen foods and dried foods, coating and packaging of foods, baking products, ice cream sieving agents, frozen foods (eg, viscosity regulators such as jams and syrups) . In addition, it is used for textile industry, paper industry, leather industry, agricultural industry, paint industry, medicine, cosmetic industry. There is no use standard for methylcellulose. However, the typical usage is less than 2% of the food, and when combined with at least one of calcium carboxyl methyl cellulose, sodium carboxyl methyl cellulose, sodium starch glycolate and sodium starch phosphate ester, the total usage is less than 2% . The method of use is to add about 1/5 of hot water to make the powder wet, add cold water and stir until it becomes a homogeneous solution. White powder or fibrous material odorless, soluble in water but not soluble in hot water. The aqueous solution is colorless transparent viscous liquid, strong against acid and alkali. Protein precipitants, silicates, carbonates, and the like. When the aqueous solution is heated, it causes a gelation phenomenon (55 to 50 ° C) and when it is cooled, it disappears and becomes an aqueous solution. It has strong adhesion and bonding force, and has unique suspension, dispersion, emulsifying power and thickening property.

&Lt; Preparation method of mixed composition >

(First Process) The mixed composition for improving the growth rate through the shiitake mushroom stimulation according to an embodiment of the present invention mixes the constituent materials at the composition ratios shown in Table 1 below.

Referring to Table 1, the average particle size of stearin magnesium is 20 nm and the composition ratio is 10 wt% (error rate ± 0.2 wt%). The potassium sorbate has an average particle size of 40 nm and a composition ratio of 30 twt% (error rate ± 0.5 wt%). In the case of magnesium carbonate, the average particle size is 80 nm and the composition ratio is 30 wt% (error rate ± 0.5 wt%). In the case of sodium alginate, the average particle size is 80 nm and the composition ratio is 15 wt% (error rate ± 0.2 wt%). In the case of methylcellulose, the average particle size is 80 nm and the composition ratio is 15 wt% (error rate ± 0.2 wt%). This composition ratio is the most preferable ratio obtained through repeated experiments.

(Second step), the nano-sized mixed composition prepared according to the first step is subjected to a laser irradiation process. This will be described in more detail below.

   (A) Prepare silicon paper. Silicon paper is prepared by cutting a thin single crystal silicon of 99.9999999% purity and smoothening the surface. Silicon paper should be free from defects and contamination, and also maintain a high level of flatness. It is preferable to use a disk-shaped silicon paper having a thickness of 0.2 mm and a diameter of 10 to 20 cm.

(B) A nano-sized mixed composition prepared by the above-described method is applied on a silicon paper so as to be evenly dispersed. However, it is preferable that the coating thickness is 0.1 mm or less.

(C) A nano-sized mixed composition is coated on silicon paper in a thickness of 0.1 mm or less, and then a laser is irradiated.

(D) The laser pulse is irradiated for 10 minutes at a wavelength of 1044 nm ± 6 nm, a frequency of 4.5 to 4.8 Hz, and an average output power of 55 to 65 W.

&Lt; Characteristics of mixed composition >

After the first and second steps described above, the Naso material composed of the mixed composition has the characteristic of stimulating the mitochondrial Crista in the mushroom cells. When applied to a mushroom using the nano-sized mixed composition thus prepared, the nanoparticles stimulate the metabolism in the mitochondria effectively to promote metabolism. This promotes cell division and increases the growth rate of shiitake mushrooms.

< Example >

1. Mixing (Mixing) of Mixture Compositions (First Process)

10 parts by weight of 'magnesium stearate' having an average particle diameter of about 20 nm, 30 parts by weight of 'potassium sorbate' having an average particle diameter of about 40 nm and magnesium carbonate having an average particle diameter of about 80 nm ), 15 parts by weight of sodium alginate having an average particle diameter of about 80 nm, and 15 parts by weight of methyl cellulose having an average particle diameter of about 80 nm.

2. Laser irradiation process (second process)

The mixed composition prepared in the first step is subjected to laser irradiation treatment in the following steps.

   (end). Prepare silicon paper. Silicon paper is manufactured by cutting thin single crystal silicon of 99.9999999% purity and smoothening the surface. Silicon paper should be free of defects or contamination, and should maintain a high level of flatness. It is recommended to use a disk-shaped one with a thickness of 0.2 mm and a diameter of 20 cm.

(I). Silicon paper A nano-sized mixed composition prepared by the above method is dispersed evenly in a certain amount. However, the coating thickness is not more than 0.1 mm.

(All). It is coated on a silicon paper with a thickness of 0.1 mm or less as described above, and then irradiated with a laser.

(la). A laser having a wavelength of 1044 nm, a frequency of 4.5 Hz and an average output power of 65 W is irradiated for 10 minutes.

3. Preparation of mixed composition solution (third step)

The water and the mixed composition are mixed at a weight ratio of 1,000: 1, and then the mixture is added to an agitator and stirred at 500 RPM for 3 minutes to prepare a mixed composition solution.

4. Mycelial growth rate experiment and mycelial density experiment

Third mycelial growth rate experiments and hyphae density of shiitake (Lentinus edodes) through the prepared mixed composition solution (Mycelial Growth and throughout the process by using Density "Water Mixture" in Lentinus edodes ) Experiments are carried out under the following conditions.

(1) Test material collection: 8 kinds (control, undiluted, 200, 400, 600, 800, 1000, 1200 times)

(2) Test design: Shiitake through "Water Mixture" ( Lentinus mycelial growth and hyphae density of edodes) (Mycelial Growth Density and by using OTM in Lentinus edodes)

(3) Mushroom basic sawdust medium composition and column repeat

 - Volume 70cc / Weight 50g

 - Cotton fiber 80% + Rice bran 20% (fully mixed and 65% controlled)

 - 3 iterations x 5 processing + Control = 18 column

 - High pressure sterilization at 121 ° C for 40 minutes

 - Petri-dish Mycelium min 1cm x 1cm peel off

 - incubation at 25 ℃ incubation room (dark)

 - Mycelial growth rate (length) measurement at 5 days interval: marked with namepen

 - When the mycelial growth is completed,

 - Mycelial density investigation: If mycelium grows compactly, +++ dogs, usually ++, weakly +

 - Compare with Control.

 - Select the most favorable treatment.

(4) Experiment 1. Experimental study on the growth rate of the mycelial growth of the mycelia by treatment with the concentration of mixed composition solution (Water Mixture)

- Disclosure strain: Sanjo 701 (921)

- Water Mixture Concentration: Control, stock solution, 200, 400, 600, 800, 1000, 1200 times

Materials and Methods: A glass column (8 treated X 3 repeats X 1 strain = 24 columns

     Oak sawdust: rice bran = 8: 2 Mixing composition solution (Water Mixture)

- Survey items: Mycelial growth rate (5-day interval check)

Table 2 shows the growth rate of the mycelia of the mycelia according to the concentration of the mixed composition solution (water mixture).

Referring to Table 2, the mycelial growth rate is measured by the size of mycelium measured 5 days after the start of the experiment. The mycelial growth rate of the stock solution is 83 ~ 89mm. 200 times is 72 ~ 78mm, 400 times is 55 ~ 59mm, 600 times is 48 ~ 50mm, 800 times is 35 ~ 40mm, 1,000 times is 22 ~ 27mm and 1,200 times is 19 ~ 22mm. The mycelial growth rate of the control was confirmed to be less than 10 mm. Therefore, when the mixed composition solution according to the present invention is used, it can be confirmed that the mycelial growth rate is faster than that of the control when diluted to 1,200 times.

(5) Experiment 2. Experimental experiment on the density of mycelial hyphae according to concentration of mixed composition solution (Water Mixture)

  - Disclosed strain:

  - OTM Concentration: Control, stock solution, 200, 400, 600, 800, 1000, 1200 times

  Materials and Methods: A glass column (8 treated X 3 repeats X 1 strain = 24 columns

     Oak sawdust: rice bran = 8: 2 Mixing Composition Solution (Water Mixture)

  - Survey items: Mycelial density (5-day interval check)

Table 3 shows the density of the hyphae hyphae according to the concentration of the mixed composition solution (water mixture).

Referring to Table 3, when the mycelial density is measured five days after the start of the experiment, the density of the superficial hyphae is very dense at the concentration of the stock solution, 200 times, 400 times, and 600 times. In the case of 800 times, 1,00 times, and 1,200 times the concentration, the normal mycelial density can be confirmed. However, we can confirm that the control hyphae density is weak.

As described above, when the mushroom is grown using the mixed composition for improving the growth rate through the shiitake mushroom crystal stimulation according to the present invention, the mycelial density can be densely grown while improving the growth rate of the mushroom.

Although the present invention has been described with reference to specific examples for illustrative purposes, the scope of the present invention is not limited to the specific embodiments of the present invention, It will be understood by those of ordinary skill in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (3)

9.8 to 10.2 wt% of magnesium stearate, 29.5 to 30.5 wt% of potassium sorbate, 29.5 to 30.5 wt% of magnesium carbonate, 14.8 to 15.2 wt% of sodium alginate, Mixture composition for improving growth rate through shiitake cristal stimulation comprising 14.8-15.2 wt% of cellulose. The method according to claim 1,
The mixed composition is prepared by applying the mixed composition to a silicon paper prepared in advance to a thickness of 0.1 mm or less,
A laser beam having a wavelength of 1038 nm to 1050 nm, a frequency of 4.5 to 4.8 Hz, and an average output optical power of 55 to 65 W is irradiated to the silicon paper coated with the mixed composition for 10 minutes,
The silicon paper is prepared by cutting a thin single crystal silicon having a purity of 99.9999999% and smoothly treating the surface. There is no defect or contamination on the surface, maintaining a flatness of a predetermined size, 20 cm. The composition for enhancing the growth rate through the shiitake mushroom crystal stimulation. The method according to claim 1,
The magnesium stearate has an average particle size of 20 nm, the potassium sorbate has an average particle size of 40 nm, the magnesium carbonate has an average particle size of 80 nm, the alginic acid Sodium alginate has an average particle size of 80 nm and the methyl cellulose has an average particle size of 80 nm. The composition for enhancing the growth rate through the shiitake mushroom crystal stimulation.