KR101880818B1 - The materials for cultivation of Fragrant Mushroom for increasing Eritadenine - Google Patents

Abstract

The present invention is a technique for increasing the eritadenine content by activating the mitochondria of mushroom using a special mixed composition for increasing the eritadenine content in the mushroom.
A method for cultivating a mushroom to increase the eritadenin content according to the present invention comprises the steps of (A) preparing an oak seedlings having a skin, (B) forming a seedling hole in the wood, (C) the timber (alley) into which the shiitake mushroom seeds have been introduced is laid down under conditions of 60% to 70% of shade and 40% to 30% of sunshine, and after 15 days, (D) stepping up the alley to the fallen season from November to December or February of the following year, and (D) cultivating the logs that have grown mycelia at least 80% E) dissolving the mixed composition according to the present invention in water to produce a mixed composition solution; and (F) repeatedly spraying the resulting mixed composition solution to the alley at intervals of 15 days until harvesting .

Description

TECHNICAL FIELD [0001] The present invention relates to a composition for cultivating shiitake mushroom having increased eritadenine content,

The present invention relates to a method for cultivating shiitake mushroom, and more particularly, to a mixed composition for cultivating shiitake mushroom which stimulates mitochondria of shiitake mushroom to promote cell metabolism and thereby increase eritadenine content.

Lentinus edodes is an edible mushroom belonging to the genus Bacillus subtilis and pine mushroom in the spring and autumn. It is a wood white rot fungus which grows on the stump and grows on the stump. Korea, China, Japan, Southeast Asia , And New Zealand (Kim et al., 2003). Shiitake mushrooms contain 81.8 ~ 90% of water, and it contains protein, amino acids, fat, ash, minerals, vitamins and dietary fiber. Dietary fiber contains fibrous components and various polysaccharides on the cell walls of mushrooms (Bartnicki-Garcia, 1968). The physiological characteristics of shiitake mushrooms include anticancer effect, antimicrobial activity, hepatitis B treatment effect, and antiviral studies. Among the studies, it has been reported that the antitumor effect of Eritadenine, a β-1,3 glucan isolated from mushroom fruiting bodies, does not have an anticancer effect directly but enhances immune function by participating in a host mediated immune response (Chihara , 1992).

The natural distribution of shiitake mushrooms is on the Korean Peninsula, Japan, China, Taiwan, the Philippines, Cerebes, New Guinea and Nepal. The natural shiitake mushroom is grown in the natural forest with the spore of the shiitake mushroom attached to the wind or the dead tree.

In the past, we took advantage of this point and used a primitive cultivation method that leaves an ax mark on the wood slice and waits for the seed to grow with it. After that, the mushroom cultivated by artificially cultivating mushroom fungus and then inoculating it into the logs has developed into a highland logging method, and the productivity of the elevation has been greatly improved.

Particularly, oak has a high density and is made of a hard material, and a method of cultivating oak mushroom using the characteristics of such oak has been developed. That is, a method of cultivating shiitake mushroom has already been developed by utilizing the method of the noi cultivation method, the method of cultivating the facility house, and the sawdust cultivation method.

However, the conventional method of cultivating shiitake mushroom is merely a method for cultivating shiitake mushroom, and studies or attempts to increase the composition of shiitake mushroom have hardly been made.

Japanese Patent Application Laid-Open No. 10-2015-0133528 (Publication date 2015.11.30) Published Japanese Patent Application No. 10-2013-0120153 (Publication date 2013.11.04)

SUMMARY OF THE INVENTION Accordingly, the present invention has been made to solve the above-mentioned problems, and it is an object of the present invention to provide a method for activating mitochondria of mitochondria using a special mixture capable of stimulating mitochondria in shiitake mushrooms to produce eritadenine, The present invention provides a mixed composition for cultivating shiitake mushroom,

Other objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.

According to another aspect of the present invention, there is provided a method of cultivating a mushroom of the present invention comprising the steps of: (A) preparing an oak seedlings having a skin; (B) forming a seedling hole in the wood, (C) a step of laying the log (alley) into which the shiitake mushroom has been introduced under the condition of 60% to 70% of shade and 40% to 30% of sunshine, (D) stepping up the alley in the fall season, which is between November and December or the following February, and (D) (E) dissolving a special mixed composition in water to produce a liquid for spraying mushroom; and (F) repeatedly spraying the generated mushroom spraying liquid onto the alley at intervals of 15 days before harvesting It is.

Preferably, the step (A) comprises: drying oak seedlings having epidermis having a diameter of 10 to 20 cm from November to February; drying the oak seedlings from the shade; and drying the dried oak timber to a length of 1 to 1.2 m And the step of cutting is carried out to the inoculation site.

Preferably, the step (C) is a step of keeping the wood (alley) into which the shiitake mushroom has been introduced in a place where ventilation and drainage are well, keeping the humidity at 70 to 80%, keeping the temperature at 20 to 28 ° C, A step of laying down a tree (alley) into which the shiitake mushroom seedlings have been laid down by one month at a time, .

Preferably, the step (D) is a step of spraying lime or veno ram wettable powder before the alley is set up, and a step of setting the distance between the logs to be about 15 cm The method comprising the steps of:

Preferably, the composition ratio of the constituent substances for increasing the eritadenine content of the mushroom in the step (E) is 19 to 21 wt% of erysorbic acid, 19 to 21 wt% of potassium sorbate, 4.5 to 5.5 wt% of potassium bromate, To 36 wt%, and sodium sulfate in an amount of 19 to 21 wt%.

Preferably, the mixed composition solution for increasing the eritadenine content of the mushroom sprayed in the step (F) is sprayed for about 10 seconds per alley.

Preferably, the step (F) comprises: a step in which a new material for cultivating mushroom for increasing the eritadenin content by erysorbic acid and potassium sorbate acts on the G protein of the mitochondrial outer membrane in the mushroom and the step of adding potassium bromate and magnesium sulfate (A) subunit of the G protein is converted to a GTP form by the sodium sulfate, and beta (gamma) -gamma (?) Subunit of the G protein by sodium sulfate activates the STE19 protein, Activating a MAP phosphorylase pathway consisting of STE19-STE9-FUS3, which acts on the inner membrane of the mitochondria and forms a complex by the interposition of the endothelial STE9 protein, and wherein the activated FUS3 phosphorylation enzyme phosphorylates the FAR5 protein Activating the cyclin-dependent protein (CDK7), and accelerating the activity of Crista to accelerate respiration and energy production .

The method of cultivating mushroom according to the present invention as described above has an effect of increasing the eritadenine content of mushroom by activating mitochondria of mushroom using a special mixed composition.

Eritadenine, an effective ingredient that promotes blood circulation and helps the blood vessels to exhaust waste, is twice as abundant as the existing shiitake mushroom, and is rich in various vitamins and fibrin. There is an effect that can be.

FIG. 1 is a flowchart for explaining a process of spraying a mixed composition solution according to the present invention on an alley.

Other objects, features and advantages of the present invention will become apparent from the detailed description of the embodiments with reference to the accompanying drawings.

A preferred embodiment of the method for cultivating shiitake mushroom according to the present invention will be described as follows. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. It is provided to let you know. Therefore, the embodiments described herein are merely the most preferred embodiments of the present invention and are not intended to represent all of the technical ideas of the present invention, so that there are various equivalents and modifications that can be substituted at the time of the present application It should be understood.

The method of cultivating the shiitake mushroom according to the present invention is as follows. First, a timber having a diameter of 10 to 20 cm is prepared from the oak with epidermis so that the lotion is rich and the moisture and hyphae are effectively protected. At this time, mycelium growth is faster than 8cm in diameter, but the flesh quality of mushroom is thin and the bending life is short. The above-mentioned logging of logs should be carried out from November to February. More specifically, logs are harvested in winter and transported to the inoculation site by cutting off a slice in February. At this time, the length of civil work is cut at 1 ~ 1.2m intervals and dried by shade.

Then, a seedling hole in which the shiitake mushroom seeds can be placed is formed in the wood, and the mushroom seeds are injected into the seed mushroom holes to be inoculated. The seeds are classified into mesophilic (10 ~ 20) and low temperature (8 ~ 18). The mesophilic property is for summer cultivation, and the low temperature is for spring and autumn cultivation (most preferred). The main cultivars are Mori 209, y602, Yujiro, etc. Inoculation of the seedlings begins in early February. Also, the size of the seed pores formed is about 13 mm, the rotation speed is preferably 6,000 ~ 10,000 RPM, and the pores are formed at intervals of about 5 cm. Also, the styrofoam stopper attached to the seeds is kept at a distance of 1 to 2 mm from the surface of the tree, and the seed hole is punched in a zigzag shape. On the other hand, seed injection (inoculation) is carried out in the shade in the mild weather with less wind.

Next, the logs (alleys) to which the mushroom seeds were injected into the seedlings were laid down under the condition of about 70% of the shade and about 30% of the shade of the shade, and after 15 days, the shade stock management was performed once a week . At this time, the bouncing is constituted by stacking of pillows or pillow stacking, and watering is prohibited until 15 days after the seedling inoculation. If the quantity of water to be fished is too small, the red mushroom will occur. If the quantity of water to be fished is exceeded, rubber mushroom will occur.

On the other hand, it is suitable to keep the temperature of 20-28 占 폚 in a place where ventilation and drainage are good, humidity of 70-80%, and inclination of the south facing direction. Then, the lumber (alley) into which the above-described tumbled mushroom seedlings are introduced is flipped once a month.

In this way, the seedlings that have been inoculated through the control of the accessory trees will have a flower pattern on the ring around June. As a result, Go ahead. At this time, before setting up the alley, lime or veno ram wetting agent is sprayed in advance, and about 20 to 30 lines per square meter are set up so that a part of the wood is downward. In other words, the distance between the logs and the logs is about 15cm in diameter.

Then, a special mixed composition for increasing the eritadenine content according to the present invention was dissolved in water (water: special mixed composition = 1000 wt%: 1 wt%) to produce a mixed composition solution, Spray repeatedly at intervals of 15 days. At this time, the mixed composition solution to be sprayed is sprayed for about 10 seconds per one alley.

The composition ratio of the constituent materials for increasing the eritadine content of the mushroom is 19 to 21 wt% of erysorbic acid, 19 to 21 wt% of potassium sorbate, 4.5 to 5.5 wt% of potassium bromate, 34 to 36 wt% of magnesium sulfate and 19 to 21 wt% of sodium sulfate %. ≪ / RTI >

The constituent materials described above will be described in detail as follows.

Erythorbic acid is used as an antioxidant to prevent food from deteriorating. It is used as a coloring aid for meat and fish, for preventing the browning of vegetables and fruits, for preventing browning by oxidization of beer.

White or yellow crystals or crystalline powder with no smell but slightly sour taste. The formula is C6H8O6. Erysorbic acid is internationally known as isoascorbic acid and is an isomer of ascorbic acid. It is not distributed in nature and does not exist in food. It is made from glucose, sucrose etc. by microorganisms. Melting point is 164 ~ 171 ℃. It is soluble in water, acetone and alcohol, slightly soluble in glycerin and insoluble in ether or benzene. It is chemically similar to ascorbic acid and has a strong reducing power, but is weak in heat and light and is characterized by decomposition by promoting oxidation remarkably by heavy metal ions such as copper ions. The aqueous erysorbic acid solution decomposes well in the air and turns black when exposed to sunlight. It is used as a coloring aid for meat and fish, for preventing the browning of vegetables and fruits, for preventing browning by oxidization of beer. In addition to these foods, it is also used as an antioxidant for butter and cheese. Be careful when using because it is weak to light and oxidized and decomposed to metal ion. Store in a sealed container that is shaded when stored. It can not be used for the purpose of prevention of oxidation, but almost all of them can be used as an antioxidant in food, and there is no limitation in the amount of use. LD50 (eld 50) is 8,300 mg / kg when administered orally to mice (experimental mice) and 18,000 mg / kg for rats (experimental rats).

Potassium sorbate is a food additive used as a preservative for processed foods by inhibiting the growth of microorganisms. Used for cheese, meat products, mushrooms, etc. White or light yellow crystalline powder with no odor or a slight odor. The formula is C6H7O2K. Melting point is 270 ℃. Sorbic acid salts have potassium, sodium and calcium salts, but potassium type is used for food because it is high in solubility, safe, and easy to manufacture. Antibacterial activity is about 74% of sorbic acid, but it dissolves well in water. It is slightly soluble in propylene glycol (5.0%) and insoluble in acetone and ether (0.1% or less). If left in air, it is oxidized and colored. It inhibits the germination and growth of microorganism spores and acts to inhibit the growth of microorganisms. It acts mainly on yeast and mold, and bacteria act selectively. The lower the pH, the higher the solubility of ingredients such as sugar and salt, the better the antibacterial effect. Cheese, processed meat products, doenjang, kochujang, red bean, dried bean curd, dried fruit, tomato ketchup, fermented beverage, fruit wine, margarine, processed sugar products. The acceptable daily intake (ADI) is 0.0 to 25.0 mg / kg bw. The LD50 (Eld 50) for mouse (experimental mouse) is 5.86 g / kg (oral) and 1.28 g / kg (subcutaneous). The degree of toxicity is not severe and no signs of carcinogenesis are present, but hyperplasia occurs when overdosed. However, when used in combination with nitrite, which is a type of coloring agent, the antimicrobial effect is increased, but DNA damaging substances are generated, which causes cancer, or causes central nerve paralysis, hemorrhagic gastritis, liver adverse effects, chromosomal abnormalities, . When used in combination with nitrite, it is safe to use in foods with a pH of 6.0 or higher.

Potassium bromate is also referred to as potassium bromate. A salt formed by the replacement of a hydrogen atom of bromic acid with potassium, which is a white crystal or powder. The formula is KBrO3. It is white crystalline or lumpy material with specific gravity of 3.27 and melting point of about 350 ℃. It decomposes by generating oxygen at about 370 ° C and turns into potassium bromide KBr. It is relatively insoluble in water at low temperatures, but is fairly soluble at high temperatures and does not dissolve in alcohol. It is easily oxidized, such as charcoal or sulfur, and explodes when heated. Bromine is added to a warm aqueous solution of potassium hydroxide or electrolysis of a warm aqueous solution of potassium bromide without using a diaphragm. It is used as an analytical reagent titrating flour decolorant and bromate, and is an oxidizing agent.

Magnesium sulfate is a sulfate of magnesium and is a white crystalline powder. It is used in medicine as a filler, mordant, etc. for paper. The formula is MgSO4. The molecular weight is 120.366 g / mol and the solubility is 35.1 g / 100 mL (20 DEG C). There are many saliva besides anhydrous. When it is usually called magnesium sulfate, it refers to the 7th water flame, which is called as the fufu salt, and the natural acid is called the salt fume (泻 利 鹽). MgSO4. The anhydrous salt is a white crystalline powder, melting at 1,185 ° C and specific gravity of 2.66. Melts at 26.9 g at 0 ° C and 68.3 g at 100 ° C in 100 g of water and slightly soluble in alcohol. The state of the hydrous salt varies depending on the temperature at which crystals are precipitated from the saturated aqueous solution. That is, a water flame is obtained from -3.9 ° C. to 1.8 ° C., a 7-water flame is obtained at 1.8 to 48.1 ° C., a 6-water flame is obtained at 48.1 to 67.5 ° C., and a single water flame is obtained at higher temperatures. It is naturally present as kisserite, and it can also be calculated as double salt, such as rubinite, polyhalide, and bentodite. It can be obtained by reacting oxides, hydroxides, carbonates and the like of magnesium with sulfuric acid, and concentrating the solution by evaporation to precipitate crystals. The kieselguhr is industrially heated at 160 ° C or higher, then dissolved in water, filtered, and then concentrated to crystallize. In addition, it is obtained by fractionation crystal (fractionation crystal) from wastewater, natural function (natural salt water), potassium industrial wastewater treatment liquid and the like. The anhydrous salt is made by red hot dehydration.

Sodium sulfate is a sodium sulfate and is a colorless crystal. Sodium sulfate in a smelting bath used industrially in the manufacture of viscose artificial silk is cooled to 4 to 7 ° C to precipitate crystals, to separate and dehydrate the product, and to produce glass or sodium sulphide. The formula is Na 2 SO 4, the molecular weight is 142.02 g / mol, and the solubility is 13.9 g / 100 mL (20 ° C.). The anhydride is a colorless crystal having a molecular weight of 142.02 and a specific gravity of 2.698. In 100 g of water, it dissolves 5 g at 0 ° C and 42 g at 100 ° C and does not dissolve in alcohol. When left in humid air, it absorbs moisture and turns into 10 hydrates. The 10 hydrate Na2SO4 · 10H2O has a specific gravity of 1.464. It becomes anhydrous in dry air and becomes anhydrous. When heated to 32.4 ℃, it melts in the crystal water and melts. When heated to 100 ℃, it becomes anhydrous. It dissolves 36 g at 15 ℃ and 412 g at 34 ℃ in 100 g of water, and it does not dissolve in alcohol like anhydrous. 7 hydrate Na2SO4 · 7H2O is obtained by cooling the supersaturated solution to below 5 ℃ or by adding alcohol. Colorless crystals are relatively stable and dissolve in 100 g of water at 44.9 g at 0 ° C and 202.6 g at 26 ° C. 10 The hydrate is also called mallow (glutinous) glabrous salt. Sodium sulfate is naturally derived from anhydrous dendrites, which are widely found in the dry regions of Western America, Canada, Central Asia, Siberia and North Africa. In laboratories, sodium hydroxide (sodium hydroxide), sodium carbonate, etc., are reacted with sulfuric acid in the laboratory. Depending on the drying method, they become hydrates and anhydrides. Industrially, sodium sulphate in a spinning bath (spinning bath component: zinc sulfate, zinc sulfate, sodium sulfate) used in the production of viscose artificial silk is cooled to 4 to 7 ° C to precipitate crystals and separate and dehydrate the product. What we have obtained is called the crystal of mankind (man silk crystallizing glauca). The purity is 93 to 95%. The anhydrides are used in the manufacture of glass or sodium sulphide, and the 10 hydrates are used as coolants and medicines in lowering agents.

As the mixed composition solution prepared according to the above-described method is repeatedly sprayed, the G protein of mitochondrial outer membrane in the mushroom cells is activated by erysorbic acid and potassium sorbate, as shown in Fig. The alpha (a) subunit of G protein is converted to GTP type by potassium bromate and magnesium sulfate, and beta (gamma) -gamma (?) Subunit of G protein activates STE19 protein by sodium sulfate.

Next, the activated STE19 protein activates the MAP kinase pathway consisting of STE19-STE9-FUS3, which acts on the inner membrane of mitochondria to form a complex by the interposition of the inner membrane STE9 protein.

And the activated FUS3 phosphorylase phosphorylates the FAR5 protein and promotes the activity of the cyclin dependent protein (CDK7). In addition, FUS3 phosphorylation stimulates mitochondrial Crista to stimulate erythadenine formation in the crystals. This can increase the content of eritadenine in shiitake mushrooms.

Erythadenine (Erithadenine) is an effective ingredient extracted from mycelium and is effective for blood pressure lowering and diabetes. It is a kind of amino acid and has been reported to be effective in blood pressure lowering because it promotes blood circulation and acts to lower cholesterol and adrenalin. In particular, it lowers cholesterol in blood, facilitates insulin secretion in the pancreas, and facilitates blood glucose control. It is also good for essential hypertension because it regulates the metabolism of corticosteroid hormone, corticosteroid, to inhibit the penetration of cholesterol into the inner wall of the blood vessel and to promote the metabolism of cholesterol and to gradually release it. It is also reported to be effective for kidney disease and gallstones.

The following Table 1 shows the components of the mushroom harvested by applying the mixed composition for cultivating mushroom of the present invention in comparison with the components of mushroom harvested by the conventional cultivation method.

Table 1. Comparison of the mushroom components according to the restoration method (per 100g of edible portion) division potassium calcium iron content Niacin protein Carbohydrate Vitamin C Folic acid Eritadenine Contents of mushroom ingredient according to existing cultivation method 256.00
mg 4.00 mg 1.3 mg 3.82 mg 2.00 g 5.51 g 5.00 g 47.00
G 42 mg The content of the mushroom ingredient sprayed with the mixed composition according to the present invention 414.5
mg 7.13 mg 2.38
mg 4.22 mg 11.2 g 12.9 g 12.09 g 58.12
G 87 mg

As shown in the following Table 1, it can be seen that the content of the mushroom cultivated up to eritadenine as well as "potassium, calcium, iron, niacin, protein, saccharide, vitamin C and folic acid" was improved. In particular, the content of erythadenine, which helps control blood sugar by promoting insulin secretion in the pancreas, helps release blood waste, promotes cholesterol metabolism and helps in the management of hypertension, It can be confirmed that it is abundant more than twice.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. It will be apparent to those skilled in the art that various modifications may be made without departing from the scope of the present invention. Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims.

Claims (2)

Characterized in that it comprises 19 to 21 wt% of erythorbic acid, 19 to 21 wt% of potassium sorbate, 4.5 to 5.5 wt% of potassium bromate, 34 to 36 wt% of magnesium sulfate and 19 to 21 wt% of sodium sulfate. A mixture for cultivation.
The method of claim 1, wherein the solution of the mixed mushroom cultivation composition of claim 1,
The step of activating the G protein of the mitochondrial outer membrane in the mushroom cells by erysorbic acid and potassium sorbate,
(A) subunit of G protein is converted to GTP type by potassium bromate and magnesium sulfate, and the beta (beta) -gamma (?) Subunit of G protein is activated by sodium sulfate to activate STE19 protein,
Activating a MAP kinase pathway comprising STE19-STE9-FUS3, wherein said activated STE19 protein acts on the inner membrane of mitochondria to form a complex by interposition of the inner membrane STE9 protein;
Wherein the activated FUS3 phosphorylase phosphorylates the FAR5 protein and activates a cyclin dependent protein (CDK7)
Wherein the FUS3 phosphorylase enzyme comprises a step of promoting erythadenine composition activity of the crystal by stimulating Crista of mitochondria.

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