KR101632393B1

KR101632393B1 - Mushroom medium and method for cultivating mushroom)

Info

Publication number: KR101632393B1
Application number: KR1020150098689A
Authority: KR
Inventors: 김태형; 김해철
Original assignee: 김태형; 김해철
Priority date: 2015-07-10
Filing date: 2015-07-10
Publication date: 2016-06-21

Abstract

The present invention relates to a medium for cultivating shiitake mushroom and a method of cultivating the shiitake mushroom using the same, and more particularly, to a method of culturing a medium for cultivation of shiitake mushroom, A method for producing a microorganism having a double structure, comprising the steps of: sealing a film formed body having a dual structure; cooling the loaded medium; cooling the film formed body of the cooled medium to inoculate the microorganism; And then removing the secondary film and the tertiary film from the browned medium, and removing the secondary and tertiary films from the browned medium, And a step of growing the removed medium.

Description

{MUSHROOM MEDIUM AND METHOD FOR CULTIVATING MUSHROOM} BACKGROUND OF THE INVENTION Field of the Invention [0001]

The present invention relates to a medium for cultivating shiitake mushroom, and a method of cultivating shiitake mushroom using the same, and more particularly, to a culture medium for shiitake mushroom cultivation which does not require shorthand operation and does not allow moisture to dry quickly, and a mushroom cultivation method using the same.

Lentinula edodes are specialties of the Orient and are rich in high quality protein, fat, various vitamins and minerals, and are treated as a nutritious food with a unique flavor. In addition, recently, pharmacological efficacy has been scientifically proven and widely recognized as a health food.

The natural distribution of shiitake mushrooms is on the Korean Peninsula, Japan, China, Taiwan, the Philippines, Cerebes, New Guinea and Nepal. The natural shiitake mushroom is grown in the natural forest with the spore of the shiitake mushroom attached to the wind or the dead tree.

In the past, we took advantage of this point and used a primitive cultivation method that leaves an ax mark on the wood slice and waits for the seed to grow with it. After that, the mushroom cultivated by artificially cultivating mushroom fungus and then inoculating it into the logs has developed into a highland logging method, and the productivity of the elevation has been greatly improved. However, when the method of planting logs is used, the utilization rate is about 10% of the plant weight, resulting in shortage of hardwood resources, and it is difficult to secure a large amount of plant weight when necessary. In addition, due to the decrease of rural labor force and the aging of growers, the method of cultivating the logs, which requires a lot of labor, is gradually becoming difficult.

Therefore, it has been used for cultivation of shiitake mushroom for a long time in cultivation of mushroom, mushroom, and mushroom. In general, rice straw, barley straw, compost, waste paper and the like are used as the medium for the method of culturing the fungus, but sawdust of the hardwood is mainly used in the cultivation method of the mushroom, which is also called sawdust cultivation.

The shiitake cultivation method of shiitake mushrooms can utilize the hardwood resources more efficiently than the log cultivation method with a utilization rate of about 30% to 40% and a utilization rate of about 10%, and the cultivation period is very short, and a lot of the cultivation process can be mechanized It is economical, and the yield of mushroom is 2-3 times as much as the method of growing wood, so it is very active now.

As a method of growing the sawdust, there is a technique of filling the sawdust medium in a synthetic resin bag and cultivating the same. In the case of such a cultivation method, it was common that the bag was incised and removed at the time when fruit bodies were produced, and then grown. However, this method requires overshooting due to excessive footing, which results in excessive labor costs due to shorthand operations. In addition, since the medium is dried quickly after the bag is removed, it is difficult to produce good quality mushrooms because the forced water supply must be continuously performed.

In order to solve the drawback of the cultivation method using the synthetic resin bag, a method of growing the mushroom by opening only the upper surface of the bag, not by removing the entire bag, has been suggested, but no obvious effect has been shown. There is a disadvantage that the cultivation amount is significantly reduced.

In addition, when mushroom is cultivated using a separate cultivation vessel, the mushroom's commerciality is remarkably lowered due to a lot of mushroom feet on the bottom and side portions of the cultivation vessel, and a shaking work is required due to a large number of feet. Of the total expenditure.

In order to solve the disadvantages of the method of cultivating shiitake mushroom, Korean Patent Registration No. 10-0875891 discloses a body clearance pipe having an inner perforation and a plurality of supply ports along an outer circumferential surface; A pedestal supporting the body clearance from the lower side; A medium compressed on the outer circumferential surface of the body clearance pipe on the upper side of the pedestal and containing mushroom bacteria therein; And a loop member formed at the upper end of the body clearance pipe. However, these mushroom cultivation vessels also consumed much time and manpower.

Korean Patent No. 10-1382018 discloses a method for producing a culture medium in which a sticking bell is provided at the lower part of a mushroom culture medium and no mushroom is generated at the lower part thereof, To produce mushrooms. However, this method does not cause mushrooms only on the underside thereof, and excessive mushrooms are formed on the side thereof, so that shorthanding is required, and proper moisture can not be continuously maintained, so that there is still a disadvantage in that forced water supply is continuously required .

KR 10-0875891 B1 KR 10-1382018 B1

Therefore, the present invention is to solve the disadvantages of the above-mentioned prior arts, and it is an object of the present invention to provide a culture medium using primary, secondary, and tertiary films so that shorthand operation is unnecessary, Thereby preventing unnecessary time and manpower from being consumed.

It also aims to reduce the cost of cultivation of mushrooms by reducing the consumption of time and manpower.

In accordance with an aspect of the present invention, there is provided a medium for cultivating a mushroom of the present invention comprising a medium composition (100), a primary film (10) surrounding the outer surface of the medium composition (100) And a tertiary film (30) surrounding the outer surface of the secondary film (20). The medium for cultivation of shiitake mushroom,
In the primary film 10 and the secondary film 20, the rudder T is formed as a single pore, seeds are inoculated through the rudder T,
A plurality of fine holes 31 are formed in the tertiary film 30 so as to allow minimal oxygen supply and carbon dioxide emission while limiting air contact,
The primary film 10 may have a thickness of 0.05 to 0.01 so that a foot may be generated during growth of the medium from which the secondary film 20 and the tertiary film 30 have been removed so that the primary film 10 can be torn out. mm. < / RTI >

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According to the present invention, shorthand work and frequent water supply are not necessary, and the cost of cultivation of shiitake mushroom can be remarkably lowered, as well as high quality mushroom cultivation is possible.

1 is a sectional view of a culture medium according to the present invention.
Fig. 2 is a view showing the process of cultivating shiitake mushroom according to the present invention. Fig.
FIG. 3 is a cross-sectional view illustrating the state of the medium according to the step of cultivating shiitake mushroom according to the present invention. FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will now be described in detail with reference to the accompanying drawings.

Conventionally, when cultivating mushrooms, high-quality mushrooms can be cultivated only by shanking due to a large number of feet. In addition, it was troublesome to continuously provide forced water to adjust the humidity to be suitable for the cultivation of shiitake mushroom.

Accordingly, the present invention provides a medium for cultivating shiitake mushroom which can reduce the inconvenience and waste of manpower and significantly reduce the cultivation cost of shiitake mushroom, and enables the cultivation of high quality mushroom, And to propose a method for doing so.

In detail, the environment in which mushrooms are generated varies depending on the temperature, moisture, relative humidity of the air, and light conditions. On average, lightness of 200 luxe, relative humidity of 90% ~ 10 ℃, the mushroom occurs well.

However, in such an environment, a large amount of mushroom feet are produced, and the moisture of the surface is considerably reduced, making it difficult to produce high quality mushrooms. Therefore, in order to overcome such disadvantages, it is necessary to limit the contact between the surface of the culture medium and the air to a minimum, in order to prevent the mushroom foot from being reduced by reducing contact with air, to be.

1, the culture medium for cultivating shiitake mushroom according to the present invention comprises a culture medium 100, a primary film 10 surrounding the outer surface of the culture medium 100, And a third film 30 surrounding the outer surface of the second film 20. The first film 10 and the second film 20 are made of a different material, (T) is provided, seeds are inoculated through the tread (T), and the tertiary film (30) is provided with a plurality of through holes (31).

That is, in the present invention, the contact between the air and the medium composition 100 is appropriately blocked by the primary film 10, the secondary film 10, and the tertiary film 30, so that the humidity is appropriately maintained. At this time, the primary film 10 serves to keep the function of the culture medium composition 100 constant by preventing moisture from passing therethrough.

Here, the primary film 10 is made of high-density polyethylene (HDPE) or the like, and prevents moisture from passing through the primary film 10, thereby maintaining the humidity of the culture medium 100 constantly maintained. In addition, the primary film 10 blocks air contact of the culture medium composition 100 to suppress excessive footing of the mushroom, thereby minimizing labor input to the stapling operation and enabling high-quality mushroom to be cultivated.

The thickness of the primary film 10 is preferably 0.05 to 0.01 mm. When the thickness of the primary film 10 is too small, the primary film 10 is easily torn or broken And if the mushroom is too thick, it will not penetrate the primary film 10 and grow.

The secondary film 20 may be formed of high density polyethylene (HDPE) or the like in the same manner as the primary film 10 to prevent the thin film-like primary film 10 from being damaged by external scratches or the like. And the thickness thereof is preferably 0.4 to 0.5 mm.

Finally, the tertiary film 30 finally encloses the medium composition 100, which helps to maintain the humidity of the medium composition 100, limits the amount of air supplied, and allows the discharge of carbon dioxide And prevents infiltration of fungi and germs. For this, a plurality of fine holes 31 are formed in the tertiary film 30.

That is, in order to inoculate the seed culture into the culture medium composition 100, a plurality of tongues (T) are formed in the primary film 10 and the secondary film 20, and the seeds are inoculated through the tongue The medium composition 100 is brought into contact with the air through the tread T to discharge carbon dioxide while being supplied with oxygen. At this time, if the third film 30 is not overlaid, contamination may occur due to a large amount of air contact. By covering the third film 10 formed with the through holes 31, excessive air contact is limited So that minimal oxygen supply and carbon dioxide emissions can be achieved.

At this time, the third film 30 is also HDPE like the secondary film 20, and its thickness is preferably 0.4 to 0.5 mm.

Here, the medium composition (100) is generally a sawdust composition used for cultivation of shiitake mushroom, and its various implementations are not limited.

As an example thereof, a medium composition (100) is prepared by making up 70 to 80% by weight of oak sawdust, 20 to 30% by weight of a raw material consisting of rice bran, wheat bran and a mixture thereof and having a water content of 60 to 70% .

The loaded medium is sterilized by autoclave at a pressure of 1 to 1.5 kg / cm 2 for 60 to 90 minutes from the time when the temperature reaches 121 캜.

The primary film 10 and the secondary film 20 are conveniently formed in the form of a cylindrical bag or a square pillar-shaped bag. The primary films 10 are put and superimposed to form the films 10 and 20 of the double structure.

The size of the film (10) (20) is not limited, and it is appropriately adjusted according to the condition of cultivation. For example, when the culture medium 100 is loaded in a cylindrical form, the total weight of the culture medium 100 is about 1.2 to 10 kg (about 100 to 250 mm) and the height is about 150 to 500 mm Of the total.

The medium for cultivating shiitake mushroom having the above-described structure is advantageous in that the foot of the mushroom is appropriately controlled and the moisture is sufficiently maintained, thereby producing a high-quality mushroom at a low cost.

Hereinafter, the composition of the culture medium and the cultivation method of the mushroom according to the present invention will be described in detail. The description of the composition of the medium is omitted.

FIG. 2 is a flow chart of cultivation of shiitake mushroom according to the present invention, and FIG. 3 is a view showing a state of a culture medium for each cultivation step, and will be described step by step with reference to FIG. 2 and FIG.

(100) for cultivation of shiitake mushroom.

First, a medium composition for cultivating shiitake is prepared. This is a general medium composition, which is composed of sawdust, material and the like, and has a moisture content of about 60 to 70%.

(10) and the secondary film (20).

Next, the composition is sealed in the primary film 10 and the secondary film 20. Here, the primary film 10 and the secondary film 20 refer to a state of being molded into a bag shape as shown in FIG. The reason why the primary film 10 and the secondary film 20 are sealed is that the primary film 10 is not inserted into the secondary film 20 after being filled in the primary film 10, And the secondary film 20 are superimposed on each other so as to have a double structure and then sealed.

The primary film 10 and the secondary film 20 may be formed at the same time to have a dual structure. Alternatively, the primary film 10 and the secondary film 20 may be formed in the same shape in the secondary film 20 ), So as to have a double structure, but the molding method and the form thereof are not limited.

Although the primary film 10 and the secondary film 20 are described in the form of a bag, the shape of the primary film 10 and the secondary film 20 is not limited as long as the opening is open and has a space therein.

Then, the inlet of the primary film 10 and the secondary film 20 is sealed, thereby blocking the contact between the medium composition 100 and air.

If the pressure is too low, the productivity is deteriorated. If the pressure is too high, the primary film 10 and the secondary film 20 may be damaged. It is because.

High-pressure sterilization of the loaded medium, followed by cooling.

Next, high pressure sterilization is performed for 60 to 90 minutes by calculating the time from when the temperature reaches 121 DEG C at a pressure of 1 to 1.5 kg / cm < 2 > using a high pressure sterilizer. Cool it in the cooling chamber and adjust it to the temperature most suitable for inoculating the mushroom seeds. At this time, the temperature is 20 占 폚 or less, preferably 15 to 20 占 폚.

The step of burying the primary film (10) and the secondary film (20) of the cooled medium and inoculating the seed bacterium.

Then, the primary film 10 and the secondary film 20 of the cooled medium are pierced to form a tongue (T), and the seeds are inoculated into the tongue (T). At this time, care should be taken not to contaminate the medium. Since the primary film 10 and the secondary film 20 are in the form of a double structure, the primary film 10 and the secondary film 20 can be simultaneously formed with the primary film 10 and the secondary film 20, will be.

The amount and method of inoculating the seeds are well known in the art to which the present invention belongs, and therefore, detailed description thereof will be omitted.

Re-enclosing the seeded inoculated medium with a tertiary film (30) having a plurality of through holes (31) formed therein.

Next, the culture medium inoculated with the seeds is re-sealed with a tertiary film 30 having a plurality of through holes 31 formed therein.

Culturing and browning the re-entrapped medium.

The re-encapsulated medium is cultured in a culture room at a temperature of about 22 to 24 ° C at an indoor carbon dioxide concentration of 1000 to 2000 ppm and at a relative humidity of 65 to 75% for 22 to 25 days. When the seed culture is visually observed, the white mycelium of mycelia grows like a lot of roots, and is firmly fused with the primary film (10) during the fusion process. In this state, when the medium is cultured for 60 to 70 days (exposed to light, 200 lux), browning occurs, and when the browning progresses, a narrow space is naturally formed between the primary film 10 and the culture medium 100 .

And removing the secondary film (20) and the tertiary film (30) from the browned medium.

When the culture is completed and browning occurs, the secondary film 20 and the tertiary film 30 are removed from the medium.

Growing the medium from which the secondary film (20) and the tertiary film (30) have been removed.

Next, it is moved to the growth chamber to create an environment suitable for growth. In this case, it is possible to minimize the shorthanding work because the foot is generated only at the portion where browning occurs, that is, the space is secured, and the primary film 10 can be torn out at the time of growth.

Proper growth conditions of shiitake mushrooms are in the range of 7 ~ 17 ℃, the daytime variability is about 8 ~ 11 ℃, the brightness is 200luxe and the relative humidity is 90% or more.

Since the culture medium is grown without removing the primary film 10, it is easy to maintain the humidity of the culture medium composition 100.

The mushrooms cultivated in this way can be shredded with a limited amount of footing, and the supply and maintenance of the moisture can be smoothly performed, so that the mushroom can be cultivated with high quality. Therefore, it is possible to generate profit through cultivation of shiitake mushroom.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit or scope of the present invention. Various modifications and variations will be possible without departing from the spirit of the invention. Therefore, the scope of the present invention should be construed as being covered by the scope of the appended claims, and technical scope within the scope of equivalents thereof should be construed as being included in the scope of the present invention.

10: primary film 20: secondary film
30: tertiary film 31: through hole
100: medium composition

Claims

A secondary film (20) surrounding the outer surface of the primary film (10), and a secondary film (20) surrounding the outer surface of the secondary composition (100) And a third film (30) surrounding the outer surface of the third film (30)
In the primary film 10 and the secondary film 20, the rudder T is formed as a single pore, seeds are inoculated through the rudder T,
A plurality of fine holes 31 are formed in the tertiary film 30 so as to allow minimal oxygen supply and carbon dioxide emission while limiting air contact,
The primary film 10 may have a thickness of 0.05 to 0.01 so that a foot may be generated during growth of the medium from which the secondary film 20 and the tertiary film 30 have been removed so that the primary film 10 can be torn out. lt; RTI ID = 0.0 > mm. < / RTI >

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