CN109097289A - One plant of white Uricularia polytricha bacterial strain - Google Patents
One plant of white Uricularia polytricha bacterial strain Download PDFInfo
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- CN109097289A CN109097289A CN201810878484.8A CN201810878484A CN109097289A CN 109097289 A CN109097289 A CN 109097289A CN 201810878484 A CN201810878484 A CN 201810878484A CN 109097289 A CN109097289 A CN 109097289A
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- uricularia polytricha
- bacterial strain
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- auricularia
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H15/00—Fungi; Lichens
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Abstract
The present invention relates to source, breeding time, yield, auricle morphological feature, molecular marker analysis of white Uricularia polytricha strains A JB1501 etc., the bacterial strain is in classify upper Auriculariaceae Auricularia Uricularia polytricha (Auricularia cornea), former name are as follows: Auricularia polytricha, in China Microbial Culture Preservation Commission's common micro-organisms collection preservation, deposit number are as follows: CGMCC14555.
Description
Technical field
The present invention relates to one plant of white Uricularia polytricha bacterial strains.
Background technique
Uricularia polytricha is a kind of edible fungus, mild-natured, sweet in flavor, has promoting production of body fluid and nourishing the lung, tonifying-Yin and nourishing-stomach, benefiting qi for tranquillization and other effects.
Have the effect of mouthfeel and tremella for being better than black fungus, be known as " hat in ear ", white Uricularia polytricha vitamin rich in and
Protein, has the function of crease-resistant and firm skin, external application can functions of removing chloasma, it is edible can " beautifying tonic ", be rare nutrition
Excellent tonic product.
Uricularia polytricha is one of China's Main Cultivation ear class, and yield occupies in edible mushroom the 5th, the Uricularia polytricha auricle cultivated at present
Color is black or brown, and white Uricularia polytricha has preferable exploitation prospect because its color is good.
Summary of the invention
The present invention provides one plant of yield height, quality white Uricularia polytricha, auricle color is not illuminated by the light influence.
White Uricularia polytricha strains A JB1501 of the invention, classification naming are cutin agaric (Auricularia cornea),
It is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is city of BeiJing, China Chaoyang District North Star west
The institute 3 of road 1, the deposit date is on September 18th, 2017, deposit number are as follows: CGMCC NO.14555.
White Uricularia polytricha strains A JB1501 of the invention is the albino mutant in 43012 bacterial strains of cultivation, acquisition white
Auricle separates through tissue and obtains strain, through experiment in cultivation in 3 years, filters out auricle color and is not illuminated by the light influence, yield is high, quality
Bacterial strain.
Detailed description of the invention
Fig. 1 is white Uricularia polytricha AJB1501 auricle morphological feature.
Fig. 2 system similar to wild type, the heredity of cultivar that is white Uricularia polytricha AJB1501 based on 25 pairs of ISSR primers
Number cluster Cluster tree.
Specific embodiment
Specific embodiment: present embodiment white Uricularia polytricha strains A JB1501, classification naming are cutin agaric
(Auricularia cornea), is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is
City of BeiJing, China Chaoyang District North Star West Road 1 institute 3, the deposit date is on September 18th, 2017, deposit number are as follows:
CGMCC14555。
Yield comparison
White Uricularia polytricha AJB1501 of the invention, wild-type strain 43012, yellow ear 10 and Shanghai 1 are taken, according to phase
Carry out inoculation yield test with cultivating bacteria bag, each bacterium bag (bacterium bag specification is Φ 22cm × 42cm, inoculation block for 1cm × 2cm ×
2cm), resulting yield result is shown in Table 1.
1 white Uricularia polytricha AJB1501 of table is compared with wild type, cultivar yield and auricle color
| Bacterial strain | Yield (kg/ bags) | Biological efficiency % | Auricle color |
| AJB1501 | 1.16 | 116.00 | White |
| Yellow ear 10 | 0.87 | 87.00 | Brown |
| Shanghai 1 | 0.78 | 78.00 | Black |
| 43012 | 0.83 | 83.00 | Black |
White Uricularia polytricha AJB1501 yield is 1.16kg/ bags, biological efficiency 116.0%, compared with wild-type strain 43012
Volume increase 39.76%, relatively produces upper cultivar Huang ear 10 and Shanghai 1 increases production 33.33%, 48.72% respectively.It can be seen that invention
Bacterial strain have the advantages that yield is high.It is white by the visible white Uricularia polytricha AJB1501 auricle (as shown in Figure 1) of auricle form
Color, is not illuminated by the light influence, dry ear be it is light yellow, auricle Dan Sheng or grow thickly, sheet, thicker, surface earless arteries and veins, transverse diameter diameter 13
~20cm indulges 9~16cm of diameter diameter, 0.16~0.2cm of thickness.
Molecular marker analysis
Test and analyze using 25 pairs of ISSR primer pair white Uricularia polytrichas and wild type, cultivar and has been shown in Table 2 and table
3, result is that genetic similarity is respectively between white Uricularia polytricha AJB1501 and wild type 43012, Huang ear 10, Shanghai 1
0.698,0.677,0.563, clustered analysis the result shows that, white Uricularia polytricha AJB1501 and wild type 43012 are in 0.61 level
On gather for same monoid (as shown in Figure 2), the more yellow ear of affiliation 10 and Shanghai 1 are close, but genetic distance is larger, is shown to be
One is different from the bacterial strain of cultivar and wild type.
Table 2ISSR primer base sequences and annealing temperature
Genetic similarity between table 3 white Uricularia polytricha AJB1501 and wild type 43012 and cultivar
| Yellow ear 10 (HER10) | AJB1501 | 43012 | Shanghai 1 (SHH1) | |
| Yellow ear 10 (HER10) | 1.000 | |||
| AJB1501 | 0.604 | 1.000 | ||
| 43012 | 0.677 | 0.698 | 1.000 | |
| Shanghai 1 (SHH1) | 0.667 | 0.563 | 0.594 | 1.000 |
Claims (1)
1. one plant of white Uricularia polytricha bacterial strain, which is characterized in that the bacterial strain is Uricularia polytricha (Auricularia cornea)
AJB1501 is deposited in China Microbial Culture Preservation Commission's common micro-organisms collection, deposit number are as follows: CGMCC14555.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810878484.8A CN109097289A (en) | 2018-08-03 | 2018-08-03 | One plant of white Uricularia polytricha bacterial strain |
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|---|---|---|---|
| CN201810878484.8A CN109097289A (en) | 2018-08-03 | 2018-08-03 | One plant of white Uricularia polytricha bacterial strain |
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| CN201810878484.8A Pending CN109097289A (en) | 2018-08-03 | 2018-08-03 | One plant of white Uricularia polytricha bacterial strain |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112359026A (en) * | 2020-11-18 | 2021-02-12 | 山东农业大学 | Method for increasing laccase content in auricularia polytricha |
| CN113667610A (en) * | 2021-09-30 | 2021-11-19 | 广西科学院 | Novel strain of horny agaric and application thereof |
| CN114667889A (en) * | 2022-04-27 | 2022-06-28 | 中国科学院昆明植物研究所 | Culture medium and culture method of white agaric |
| CN115029254A (en) * | 2022-06-24 | 2022-09-09 | 四川省中医药科学院 | Novel auricularia polytricha strain and extract and application thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112359026A (en) * | 2020-11-18 | 2021-02-12 | 山东农业大学 | Method for increasing laccase content in auricularia polytricha |
| CN112359026B (en) * | 2020-11-18 | 2022-08-19 | 山东农业大学 | Method for increasing laccase content in auricularia polytricha |
| CN113667610A (en) * | 2021-09-30 | 2021-11-19 | 广西科学院 | Novel strain of horny agaric and application thereof |
| CN114667889A (en) * | 2022-04-27 | 2022-06-28 | 中国科学院昆明植物研究所 | Culture medium and culture method of white agaric |
| CN115029254A (en) * | 2022-06-24 | 2022-09-09 | 四川省中医药科学院 | Novel auricularia polytricha strain and extract and application thereof |
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