CN115029254A - Novel auricularia polytricha strain and extract and application thereof - Google Patents
Novel auricularia polytricha strain and extract and application thereof Download PDFInfo
- Publication number
- CN115029254A CN115029254A CN202210725308.7A CN202210725308A CN115029254A CN 115029254 A CN115029254 A CN 115029254A CN 202210725308 A CN202210725308 A CN 202210725308A CN 115029254 A CN115029254 A CN 115029254A
- Authority
- CN
- China
- Prior art keywords
- auricularia polytricha
- strain
- food
- extract
- auricularia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 240000005710 Auricularia polytricha Species 0.000 title claims abstract description 80
- 235000000024 Auricularia polytricha Nutrition 0.000 title claims abstract description 80
- 239000000284 extract Substances 0.000 title claims abstract description 24
- 210000004369 blood Anatomy 0.000 claims abstract description 26
- 239000008280 blood Substances 0.000 claims abstract description 26
- 230000001603 reducing effect Effects 0.000 claims abstract description 18
- 239000003814 drug Substances 0.000 claims abstract description 12
- 238000004321 preservation Methods 0.000 claims abstract description 12
- 238000009629 microbiological culture Methods 0.000 claims abstract description 10
- 244000005700 microbiome Species 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 239000000706 filtrate Substances 0.000 claims description 19
- 238000001914 filtration Methods 0.000 claims description 18
- 239000006286 aqueous extract Substances 0.000 claims description 15
- 238000010438 heat treatment Methods 0.000 claims description 14
- 238000010992 reflux Methods 0.000 claims description 14
- 235000013305 food Nutrition 0.000 claims description 13
- 235000013402 health food Nutrition 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 8
- 239000000825 pharmaceutical preparation Substances 0.000 claims description 7
- 239000004480 active ingredient Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 4
- 150000002632 lipids Chemical class 0.000 claims description 3
- 229940127557 pharmaceutical product Drugs 0.000 claims 4
- 239000002671 adjuvant Substances 0.000 claims 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 8
- 229940079593 drug Drugs 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 8
- 210000002966 serum Anatomy 0.000 abstract description 8
- 108010023302 HDL Cholesterol Proteins 0.000 abstract description 6
- 108010028554 LDL Cholesterol Proteins 0.000 abstract description 6
- 235000012000 cholesterol Nutrition 0.000 abstract description 4
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 abstract description 3
- 235000019197 fats Nutrition 0.000 description 16
- 229940124647 MEK inhibitor Drugs 0.000 description 7
- 150000004676 glycans Chemical class 0.000 description 7
- 239000005017 polysaccharide Substances 0.000 description 7
- 229920001282 polysaccharide Polymers 0.000 description 7
- 102100031480 Dual specificity mitogen-activated protein kinase kinase 1 Human genes 0.000 description 6
- 101710146526 Dual specificity mitogen-activated protein kinase kinase 1 Proteins 0.000 description 6
- 241000700159 Rattus Species 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 208000032843 Hemorrhage Diseases 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000221377 Auricularia Species 0.000 description 2
- 102100023274 Dual specificity mitogen-activated protein kinase kinase 4 Human genes 0.000 description 2
- 208000002193 Pain Diseases 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 235000019784 crude fat Nutrition 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 231100000862 numbness Toxicity 0.000 description 2
- 230000000144 pharmacologic effect Effects 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 241000221454 Auriculariaceae Species 0.000 description 1
- 208000008035 Back Pain Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000019750 Crude protein Nutrition 0.000 description 1
- 102100023266 Dual specificity mitogen-activated protein kinase kinase 2 Human genes 0.000 description 1
- 101710146529 Dual specificity mitogen-activated protein kinase kinase 2 Proteins 0.000 description 1
- 102100023275 Dual specificity mitogen-activated protein kinase kinase 3 Human genes 0.000 description 1
- 101710146518 Dual specificity mitogen-activated protein kinase kinase 4 Proteins 0.000 description 1
- 102100023272 Dual specificity mitogen-activated protein kinase kinase 5 Human genes 0.000 description 1
- 208000012671 Gastrointestinal haemorrhages Diseases 0.000 description 1
- 101001115394 Homo sapiens Dual specificity mitogen-activated protein kinase kinase 3 Proteins 0.000 description 1
- 101001115395 Homo sapiens Dual specificity mitogen-activated protein kinase kinase 4 Proteins 0.000 description 1
- 101001115390 Homo sapiens Dual specificity mitogen-activated protein kinase kinase 5 Proteins 0.000 description 1
- 208000004044 Hypesthesia Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 208000008930 Low Back Pain Diseases 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 206010027514 Metrorrhagia Diseases 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 208000007101 Muscle Cramp Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010067171 Regurgitation Diseases 0.000 description 1
- 206010040943 Skin Ulcer Diseases 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 208000003217 Tetany Diseases 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 239000002956 ash Substances 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 150000001746 carotenes Chemical class 0.000 description 1
- 235000005473 carotenes Nutrition 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000015271 coagulation Effects 0.000 description 1
- 238000005345 coagulation Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 231100000673 dose–response relationship Toxicity 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 230000004217 heart function Effects 0.000 description 1
- 208000035861 hematochezia Diseases 0.000 description 1
- 208000014617 hemorrhoid Diseases 0.000 description 1
- 230000000055 hyoplipidemic effect Effects 0.000 description 1
- 238000011629 hyperlipidemia animal model Methods 0.000 description 1
- 208000034783 hypoesthesia Diseases 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 231100000019 skin ulcer Toxicity 0.000 description 1
- NRHMKIHPTBHXPF-TUJRSCDTSA-M sodium cholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 NRHMKIHPTBHXPF-TUJRSCDTSA-M 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229960004793 sucrose Drugs 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
- A61P3/06—Antihyperlipidemics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- Wood Science & Technology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Obesity (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Diabetes (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Epidemiology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides an Auricularia polytricha strain (Auricularia polytricha), which is preserved in the China general microbiological culture Collection center of the Committee for culture Collection of microorganisms with the preservation number of CGMCC NO. 23834. The auricularia polytricha strain has excellent blood fat reducing effect, the extract of the auricularia polytricha strain can effectively reduce the contents of total cholesterol, triglyceride, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol in serum, and the blood fat is effectively reduced, the effect is better than that of a plurality of wild auricularia polytricha and the auricularia polytricha strain which is preserved in the China general microbiological culture Collection center with the preservation number of CGMCC No.40184, and the auricularia polytricha strain has very good application value in preparing health care products for assisting in reducing blood fat or medicines for reducing blood fat.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to a novel auricularia polytricha strain, an extract and application thereof.
Background
Auricularia polytricha (Auricularia polytricha) is a fungus belonging to Auricularia species in Auricularia of Auriculariaceae, and is an edible fungus with easy cultivation and low cost. Auricularia polytricha has long been used as a medicine, and has the effects of benefiting qi, strengthening body, promoting blood circulation, stopping bleeding and relieving pain. Can be used for treating cold and dampness lumbago and skelalgia, puerperal asthenia, cramp and numbness, pain due to trauma, blood vessel obstruction, numbness, tetany, leukorrhagia, hematochezia, hemorrhoid hemorrhage, metrorrhagia, regurgitation, excessive phlegm, and senile skin ulcer. It can also be used for treating toxic bacteria poisoning.
The auricularia polytricha contains various nutritional components such as various amino acids, carotene, ascorbic acid and the like, and long-term research shows that various pharmacological effects of the auricularia polytricha are closely related to auricularia polytricha polysaccharide. The auricularia polytricha is extracted by hot water at about 80 ℃, protein is removed by a Sevage method, dialysis, concentration and drying are carried out after ethanol precipitation to obtain auricularia polytricha polysaccharide, and researches show that the auricularia polytricha polysaccharide has various pharmacological effects of stopping bleeding, resisting coagulation, reducing blood fat, resisting aging, protecting body cells, improving cardiac function, regulating immunity, resisting tumors and the like.
At present, a plurality of researches aiming at the extraction process of auricularia polytricha polysaccharides are reported (for example [1] hungry swallow, Yumengyao, Jiangnan, and the like. an orthogonal test method is used for optimizing the extraction process of auricularia polytricha polysaccharides [ J ]. Chinese edible fungi, 2013,32(1): 41-44; [2] Wangwei, Chenfan, Wangying Yuling. the research of the extraction process of auricularia polytricha polysaccharides [ J ]. proceedings of the institute of faculty, Zhangzhou (Nature science edition), 2009,22(3):121-124.DOI: 10.3969/j.snis.1008-7826.2009.03.027.). However, for the blood lipid lowering effect, besides being related to the extraction process of auricularia polytricha polysaccharide, the most essential is closely related to the quality of the auricularia polytricha. The existing wild auricularia polytricha is limited by the content of active ingredients, and the blood fat reducing efficacy of products (powder, extract and the like) prepared from the wild auricularia polytricha is still insufficient.
Therefore, the new strain of the auricularia polytricha with excellent blood fat reducing effect has very excellent application value in preparing health care products for assisting in reducing blood fat or medicines for reducing blood fat.
Disclosure of Invention
The invention aims to provide a new auricularia polytricha strain with excellent hypolipidemic effect, which is preserved in China general microbiological culture Collection center (CGMCC), the preservation address is No. 3 of the Beijing market Hokko No. 1 North Chen West Lu, the preservation number is CGMCC NO.23834, the preservation date is as follows: 10/29/2021, classified and named as: auricularia polytricha.
The invention provides an Auricularia polytricha strain (Auricularia polytricha), which is preserved in the China general microbiological culture Collection center of the Committee for culture Collection of microorganisms with the preservation number of CGMCC NO. 23834.
The invention also provides an auricularia polytricha water extract which is the water extract of the auricularia polytricha strain.
Further, the water extract of auricularia polytricha is prepared by the method comprising the following steps:
(1) adding water into the auricularia polytricha strain, and heating and refluxing for 1-5 hours;
(2) filtering while the solution is hot to obtain filtrate.
Still further, the above method further comprises the steps of:
(3) and (3) adding water into the filter residue obtained by filtering, heating and refluxing for 1-5 hours, filtering while the filter residue is hot to obtain a filtrate, and combining the filtrate with the filtrate obtained in the step (2).
The invention also provides application of the auricularia polytricha strain or the extract thereof in preparing food, health-care food or medicines.
The invention also provides a food, a health food or a medicine, which is prepared by taking the powder of the auricularia polytricha strain and/or the extract thereof as active ingredients and adding food auxiliary materials, or a pharmaceutical preparation prepared by adding pharmaceutically acceptable auxiliary materials.
Further, the active ingredient is an aqueous extract of Auricularia polytricha strain.
Further, the aqueous extract of the Auricularia polytricha strain is prepared by the following steps:
(1) adding water into the auricularia polytricha strain, and heating and refluxing for 1-5 hours;
(2) filtering while the solution is hot to obtain filtrate.
Still further, the above method further comprises the steps of:
(3) and (3) adding water into the filter residue obtained by filtering, heating and refluxing for 1-5 hours, filtering while the filter residue is hot to obtain a filtrate, and combining the filtrate with the filtrate obtained in the step (2).
Further, the health food is a health food for assisting in reducing blood fat; the medicine is used for reducing blood fat.
The invention has the beneficial effects that: the novel auricularia polytricha strain provided by the invention has an excellent blood fat reducing effect, the extract of the novel auricularia polytricha strain can effectively reduce the contents of total cholesterol, triglyceride, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol in serum, and the blood fat is effectively reduced, the effect is superior to that of a plurality of wild auricularia polytricha strains and auricularia polytricha strains which are preserved in the common microorganism center of China Committee for culture Collection of microorganisms and management and have the preservation number of CGMCC No.40184, and the novel auricularia polytricha strain has very excellent application value in preparing health care products for assisting in reducing blood fat or medicines for reducing blood fat.
Obviously, many modifications, substitutions, and variations are possible in light of the above teachings of the invention, without departing from the basic technical spirit of the invention, as defined by the following claims.
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Detailed Description
The raw materials and equipment used in the invention are known products and are obtained by purchasing commercial products.
Example 1 preparation of aqueous extract of Auricularia polytricha of the invention
Taking 30g of auricularia polytricha MEK1 (preserved in China general microbiological culture Collection center with the preservation number of CGMCC NO.23834), placing in a round-bottomed flask, adding 1000mL of water, standing for 1 hour, heating and refluxing for 3 hours, filtering while hot, washing a filter and filter residues with a small amount of hot water, placing the filter residues and filter paper in the flask, adding 1000mL of water, heating and refluxing for 3 hours, filtering while hot, combining the filtrates, and concentrating under reduced pressure at 60 ℃ to 100mL to obtain the extract.
Example 2 preparation of aqueous extract of Auricularia polytricha of the invention
Taking 30g of auricularia polytricha MEK1 (preserved in China general microbiological culture Collection center with the preservation number of CGMCC NO.23834), placing in a round-bottomed flask, adding 1000mL of water, standing for 1 hour, heating and refluxing for 1 hour, filtering while hot, washing a filter and filter residues with a small amount of hot water, placing the filter residues and filter paper in the flask, adding 1000mL of water, heating and refluxing for 1 hour, filtering while hot, combining the filtrates, and concentrating under reduced pressure at 60 ℃ to 100mL to obtain the extract.
Example 3 preparation of aqueous extract of Auricularia polytricha of the present invention
Taking 30g of auricularia polytricha MEK1 (preserved in China general microbiological culture Collection center with the preservation number of CGMCC NO.23834), placing the auricularia polytricha MEK1 in a round-bottomed flask, adding 1000mL of water, standing for 1 hour, heating and refluxing for 5 hours, filtering while hot, washing a filter and filter residues with a small amount of hot water, placing the filter residues and the filter paper in the flask, adding 1000mL of water, heating and refluxing for 5 hours, filtering while hot, combining the filtrates, and concentrating under reduced pressure at 60 ℃ to 100mL to obtain the extract.
Comparative example 1 preparation of aqueous extract of Auricularia polytricha
The extract of Auricularia polytricha MEK2 (wild isolated Auricularia polytricha) was prepared in the same manner as in example 1, except that 30g of the extract was used.
Comparative example 2 preparation of aqueous extract of Auricularia polytricha
The extract of Auricularia polytricha MEK3 (wild isolated Auricularia polytricha) was prepared in the same manner as in example 1, except that 30g of the extract was used.
Comparative example 3 preparation of aqueous extract of Auricularia polytricha
An extract was prepared from 30g of auricularia polytricha MEK5 (deposited in China general microbiological culture Collection center, accession number CGMCC NO.40184) by the method of example 1.
The beneficial effects of the present invention are demonstrated by the following experimental examples.
Experimental example 1 Effect of Auricularia polytricha aqueous extract on Mixed type hyperlipidemia animal model
1. Method for producing a composite material
1.1 model feed
The maintenance feed is added with 20.0% of cane sugar, 15% of lard, 1.2% of cholesterol, 0.2% of sodium cholate, and proper amounts of casein, calcium hydrophosphate, stone powder and the like. In addition to crude fat, moisture, crude protein, crude fat, crude fiber, crude ash, calcium, phosphorus, calcium of the model feed: phosphorus is required to meet the national standards for maintaining feed.
1.2 Experimental procedures
Male SD rats (200 ± 20g) were randomly divided into 5 groups, including three dose groups (0.5, 1, 2g of crude drug/kg), a blank control group and a model control group, 10 rats per group.
The test samples (example 1, concentration adjusted to different doses) were orally administered to the three dose groups daily in a volume of 10ml/kg, the blank control group and the model control group were simultaneously administered with the same volume of distilled water, the blank control group was administered with the maintenance feed, and the model control group and the three dose groups were administered with the model feed, and the body weights were regularly weighed. The administration was continued for 30 days. Blood was collected on day 31 without fasting from the tail tip, and serum was centrifuged to determine the levels of TC, TG and LDL-C, HDL-C in the serum.
In the same manner, the drugs of comparative examples 1 to 3 were administered to 10 mice each in 3 dose groups (0.5, 1, 2g crude drug/kg).
2. Results
The results are shown in table 1, after the rats are fed with the model feed for 30 days, the serum concentrations of TC, TG and LDL-C of the rats in the model control group are remarkably increased (p is less than 0.01) compared with that in the blank control group, and the concentration of HDL-C is reduced (p is less than 0.01) compared with that in the blank control group. The 3 dose groups of the extracts of the auricularia polytricha MEK1(CGMCC NO.23834) and MEK4 can obviously reduce the concentrations of TC, TG and LDL-C (p is less than 0.05, p is less than 0.01) in the serum of rats and increase the concentration of HDL-C (p is less than 0.05, p is less than 0.01) in the serum, are dose-dependent and have good blood fat reducing effect. The MEK1(CGMCC NO.23834) has better blood lipid-lowering effect than MEK 4.
TABLE 1 Effect of Auricularia polytricha extract on rat blood lipids
Remarking: **: the difference was very significant compared to the blank control group (p < 0.01); and (delta): significant difference compared to model control group (p < 0.05); and delta: compared with the model control group, the difference is very significant (p <0.01)
In conclusion, the new auricularia polytricha strain provided by the invention has an excellent blood fat reducing effect, the extract of the new auricularia polytricha strain can effectively reduce the contents of total cholesterol, triglyceride, low-density lipoprotein cholesterol and high-density lipoprotein cholesterol in serum, and can effectively reduce blood fat, the effect is superior to that of a plurality of wild auricularia polytricha and the auricularia polytricha strain which is preserved in China general microbiological culture Collection center and has the preservation number of CGMCC No.40184, and the new auricularia polytricha strain has very excellent application value in the preparation of health care products for assisting in reducing blood fat or medicines for reducing blood fat.
Claims (10)
1. An Auricularia polytricha strain (Auricularia polytricha) is characterized by being preserved in the China general microbiological culture Collection center of China Committee for culture Collection of microorganisms with the preservation number of CGMCC NO. 23834.
2. An aqueous extract of Auricularia polytricha which is an aqueous extract of the Auricularia polytricha strain of claim 1.
3. The aqueous extract of auricularia polytricha as claimed in claim 2, which is prepared by a process comprising the steps of:
(1) adding water into the auricularia polytricha strain, and heating and refluxing for 1-5 hours;
(2) filtering while the solution is hot to obtain filtrate.
4. The aqueous extract of auricularia polytricha as claimed in claim 3, wherein said method further comprises the steps of:
(3) and (3) adding water into the filter residue obtained by filtering, heating and refluxing for 1-5 hours, filtering while the filter residue is hot to obtain a filtrate, and combining the filtrate with the filtrate obtained in the step (2).
5. Use of the Auricularia polytricha strain or extract thereof of claim 1 in the preparation of a food, health food or pharmaceutical product.
6. A food, health food or pharmaceutical characterized in that it is a food, health food or pharmaceutical preparation prepared by adding a food adjuvant to the powder of the Auricularia polytricha strain of claim 1 and/or its extract as an active ingredient, or a pharmaceutical preparation prepared by adding a pharmaceutically acceptable adjuvant to the food or health food.
7. The food, health food or pharmaceutical product according to claim 6, wherein the active ingredient is an aqueous extract of Auricularia polytricha strain.
8. The food, health food or pharmaceutical product of claim 7, wherein said aqueous extract of the strain of Auricularia polytricha is prepared by a process comprising:
(1) adding water into the auricularia polytricha strain, and heating and refluxing for 1-5 hours;
(2) filtering while the solution is hot to obtain filtrate.
9. The food, health food or pharmaceutical product according to claim 8, wherein the method further comprises the steps of:
(3) and (3) adding water into the filter residue obtained by filtering, heating and refluxing for 1-5 hours, filtering while the filter residue is hot to obtain a filtrate, and combining the filtrate with the filtrate obtained in the step (2).
10. The food, health food or pharmaceutical of claim 6, wherein the health food is a health food for assisting in lowering blood lipid; the medicine is used for reducing blood fat.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210725308.7A CN115029254A (en) | 2022-06-24 | 2022-06-24 | Novel auricularia polytricha strain and extract and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210725308.7A CN115029254A (en) | 2022-06-24 | 2022-06-24 | Novel auricularia polytricha strain and extract and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115029254A true CN115029254A (en) | 2022-09-09 |
Family
ID=83126083
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210725308.7A Pending CN115029254A (en) | 2022-06-24 | 2022-06-24 | Novel auricularia polytricha strain and extract and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115029254A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103214551A (en) * | 2013-01-08 | 2013-07-24 | 北京市农林科学院 | Preparation method and applications of auricularia polytricha blood fat reduction function protein extract |
| CN103417583A (en) * | 2013-08-09 | 2013-12-04 | 北京市农林科学院 | Auricularia polytricha extractive and a preparation method and application thereof |
| CN109097289A (en) * | 2018-08-03 | 2018-12-28 | 四川省农业科学院土壤肥料研究所 | One plant of white Uricularia polytricha bacterial strain |
-
2022
- 2022-06-24 CN CN202210725308.7A patent/CN115029254A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103214551A (en) * | 2013-01-08 | 2013-07-24 | 北京市农林科学院 | Preparation method and applications of auricularia polytricha blood fat reduction function protein extract |
| CN103417583A (en) * | 2013-08-09 | 2013-12-04 | 北京市农林科学院 | Auricularia polytricha extractive and a preparation method and application thereof |
| CN109097289A (en) * | 2018-08-03 | 2018-12-28 | 四川省农业科学院土壤肥料研究所 | One plant of white Uricularia polytricha bacterial strain |
Non-Patent Citations (4)
| Title |
|---|
| PING LI等: "Gamma-Irradiation-Induced Degradation of the Water-Soluble Polysaccharide from Auricularia polytricha and Its Anti-Hypercholesterolemic Activity" * |
| 吴春敏等: "毛木耳多糖的抗凝血和降血脂作用" * |
| 张丹等: "毛木耳(Auricularia polytricha)的研究进展" * |
| 赵爽等: "毛木耳多糖降血脂功效研究" * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1902722B1 (en) | Agent for elevating adiponectin concentration | |
| CN101822373B (en) | Golden-fungus gingko functional food, preparation method and use thereof | |
| WO2020177389A1 (en) | Ergothioneine-containing hericium erinaceus health product formulation and preparation method therefor | |
| CN101249259B (en) | High content and high activity oral polysaccharide-peptide and preparing method and application of the same | |
| KR20080071016A (en) | Method for extracting beta-glucan from astragali radix and compositions for feed additives comprising the beta-glucan | |
| WO2016204120A1 (en) | Method for producing fermented bamboo extract and immunostimulator | |
| CN112425684B (en) | Fermented feed additive with immunity enhancing effect and preparation method thereof | |
| CN112535239A (en) | Compound Chinese herbal medicine feed additive for daily health care and growth promotion of livestock and preparation and application thereof | |
| CN115337331B (en) | Preparation method of antidiabetic Chinese holly She Gutai fermented product and liquid fermented product | |
| KR101784093B1 (en) | Manufacturing method of composition having anti-diabetic effect containing the extracts of fermented germinated brown rice and orstachys japonia | |
| JP4743947B2 (en) | Lipid metabolism improving composition | |
| CN115029254A (en) | Novel auricularia polytricha strain and extract and application thereof | |
| CN113350310B (en) | Tea pigment capsule with blood sugar and blood fat reducing function and preparation method thereof | |
| CN101755863A (en) | Honeysuckle noodle and production method thereof | |
| CN108066439A (en) | A kind of preparation method of the reducing blood lipid native compound group based on solution fermentation | |
| CN103271968A (en) | Blood glucose reduction use of kardiseed dreg extractive | |
| KR20220020147A (en) | Method for preparing Phellinus linteus mycelia extract or Phellinus linteus mycelia extract powder and composition for enhancing immunity comprising them as an active ingredient | |
| CN106260359A (en) | A kind of Saussurea involucrata culture compositions Ganoderma tea and preparation method thereof | |
| CN112754014A (en) | Composition for preventing and treating cardiovascular and cerebrovascular diseases and preparation method thereof | |
| CN111514250A (en) | Compound beverage with blood fat reducing effect and preparation method and application thereof | |
| CN111991521A (en) | Tea buccal tablet for reducing high blood pressure, high blood sugar and high blood lipid and preparation method thereof | |
| CN114097906B (en) | Rake fungus fermented tea and preparation method thereof | |
| KR20120132646A (en) | Liquid cultivating method of Phellinus linteus increasing antioxidant and immunostimulating activity | |
| CN116236440B (en) | Preparation method of astragalus polysaccharide oral liquid with high immunocompetence for chickens and product thereof | |
| CN115006489B (en) | Fermented rhizoma polygonati extract with blood pressure reducing effect, preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220909 |