TWI393530B - The method of inoculation and cultivation of - Google Patents

Description

Inoculation and cultivation method of Ox

The present invention relates to a method for inoculation and cultivation of Antrodia camphorata, in particular, the use of a closed cultivation bucket to form an ecological circle for the cultivation of Burdock, and the cultivation process thereof is carried out by using the burdock itself. The essential oil can effectively remove the bacteria and preserve the original strain attached to the burdock wood to achieve the target effect of sterilization, so as to promote the activity of the bio-competitive instinct and the activated strain in the culture barrel (B. bursii), and help the bacteria The silk body culture is grown into the best mushroom of the A. camphorata fruit body, and the method of inoculating and cultivating the A. sinensis is simplified.

According to the present, it is now possible to cultivate the production equipment of the mushroom body of the growth of A. angustifolia. Generally, a large amount of the burdock wood is placed on the shelf in a small amount of the batch packaging device, and the mechanical cleaning bucket and the automatic filtering device are used. After cleaning and high-temperature sterilization, the segmental wood is sent into a closed cultivation room of a large environmental space in batches, and the segmental wood is arranged one by one in the cultivation frame of the cultivation room, so that the segment of wood is in the cultivation room. In the artificial environment with the specified temperature and humidity, the mushroom of the body of the Antrodia camphorata grows to the harvest; however, in the process of cultivating and producing, it is necessary to go through multiple equipments, and the process of each equipment is not consistent. And its high-temperature sterilization axe can achieve 100% complete sterilization, but the mycelium will be intimately mated during the process of implanting single or artificial process bacteria, and finally it will lose its activity and become sluggish, and it is difficult to grow good burdock fruit body. Therefore, it has not been systematic. The cultivation method is very complicated and trivial. It will inevitably take time to improve the labor costs, and cultivate the indoor environment. Degree and humidity are difficult to detect during the cultivation process As soon as the conditions for cultivating the environment are not properly controlled, it will affect the quality of the mushrooms of each batch of A. sylvestris fruit, resulting in low production yield.

The present inventors have invented the present invention in view of the above, and the idea of creation and creation, which has been designed with many years of experience, and has been explored and tested by various parties, and has been modified several times.

The main object of the present invention is to provide a method for inoculating and cultivating the burdock wood, which is prepared by stacking the burdock wood after the steps of polishing the skin, cleaning, sterilizing, resterilizing, and inoculating the culture dish. The culture is carried out in a closed cultivation tank containing an appropriate amount of water. The fungus is anaerobic and the carbon dioxide in the confined space can be sterilized to effectively inhibit the formation of bacteria, and is used to decompose the burdock wood during the hypha growth in the closed barrel. The carbon and nitrogen source (ratio) of the trace amount of biogas generated in the xylem can be used to increase the growth of the mycelium and promote the growth of the fruiting body, thereby simplifying the cultivation process of the oyster mushroom and effectively reducing the expenditure of various manpower and equipment.

Another object of the present invention is to provide a method for inoculating and cultivating Antrodia camphorata, wherein the burdock essential oil extracted from the burdock wood can be used to effectively remove the bacteria and preserve the native strain attached to the burdock. In order to achieve the target effect of sterilization, and the same kind of strain will not kill each other, will compete with each other and breed the next generation, and can reach the bio-competitive instinct and activated strain of various bacteria (B. bursii) in the culture barrel. Activity, accelerated growth and mass production of secondary products (B. chinensis).

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The content is as follows: as shown in the first figure is the flow chart of the method of the present invention, firstly, according to the ratio of the preset volume (material) ratio of 5:3:0.2:0.05, the material is taken as a breeding bucket (10) , volume of 500 liters), appropriate amount of burdock wood (20, volume of 300 liters), water (30, volume of 20 liters) and burdock extract of burdock essential oil (40, volume of 5 liters), first, the Department The pre-treatment process of the wood (20) is carried out as follows: the pre-cut burdock wood (20) is used to cut the burdock wood (20): the first polished skin procedure : The surface layer of the burdock wood (20) is polished to remove the surface bacteria, so that the capillary holes of the segment wood (20) are opened; the second cleaning procedure: the wood (20) of the polished skin is Rinse with clean water; the third sterilization procedure: take one of the pure burdock essential oil (40) extracted from the burdock itself and spray it directly on the burdock wood (20) for sterilization; Essential oil (40) itself has a bactericidal effect to effectively remove the bacteria and the same species will not kill each other and will compete with each other. And the reproduction of the excellent next-generation characteristics, which effectively promotes the preservation of the native species attached to the burdock, in order to achieve the target effect of sterilization and achieve the diversity of bacteria in the culture barrel (Bursui), and accelerate the activity of the biological competition instinct and the activated species. Growth and mass production of secondary products (Arctium edodes); fourth re-sterilization procedure: sterilized burdock wood (20) sprayed with burdock essential oil (40) for high temperature sterilization; fifth culture dish Inoculation procedure: take the hyphae of the burdock culture dish, and transfer the mycelium layer to the surface of the section of wood (20) to complete the preliminary burdock wood The pretreatment process of the material (20) is carried out; then, the cultivation process is carried out, and the steps are as follows: Step (1) Washing the barrel: taking the burdock essential oil extracted from the burdock wood, and washing the barrel of the liter of 500 liters (10) Net, in the cultivation barrel (10), a plurality of hollow blocks (12) are arranged in a hollow cylinder, and are arranged on the bottom surface of the cultivation barrel (10) to form a cushion layer having a predetermined height from the bottom surface (121). Preliminary spray sterilization on the inner surface of the barrel; Step (2) into the water: Pour 20 liters of water (30) into the incubator (10) so that the water level in the barrel is lower than the height of the block (12) Height, so that the elevated block (12) is stretched out on the water surface to form a cushion layer (121) with a predetermined height from the bottom surface, and then the burdock essential oil extracted from the burdock wood is again applied to the inner surface of the barrel body. Spray sterilization; Step (3) water sterilization: Take 5 liters of burdock essential oil (40) into the cultivation tank (10), using the specific gravity of oil and water, the essential oil will float on the surface of the water and form a barrel. The internal water source is sterilized to form a breeding room (11) with a sterile space in the cultivation tank (10); to help the mycelium growth of the Antrodia camphorata while providing the water in the elimination water Steps (4) Put in the section of wood: in the cultivation tank (10), place the high block (12) on the cushion layer (121), and make the 300 liters of the burdock of the material taken beforehand. And the wood (20) of each section after initial planting is placed on the cushion (121) in order from the bottom to the top; Step (5) closed the cultivation barrel: the barrel of the cultivation barrel (10) Covering a transparent mold cover (13) and a bucket cover (14), the internal cultivation chamber (11) is formed into a closed space, so that the segment wood (20) is placed in the incubation chamber (11); Step (6) Static culture: use the water (30) on the bottom surface of the barrel of the cultivation bucket (10). The incubation room (11) is a confined space, and the internal humidity is attached to the transparent mold cover (13) by water vapor to form a water droplet, and then the water droplets are photographed by tapping the transparent mold cover (13). This cycle forms a breeding bucket for the artificial rainforest ecosystem. When the mycelium is grown and fermented, the carbon and nitrogen sources (ratio) of the biogas are naturally generated, which is beneficial to the growth of the mycelium and the growth of the fruiting bodies. Element, and because the bacteria and fungi require oxygen and the fungus is anaerobic, the oxygen in the incubation room (11) naturally occurs due to the fermentation of the mycelium growing process, which causes oxygen consumption and hyphal growth to decompose and decompose the xylem. The carbon and nitrogen source (ratio) of trace biogas increases the carbon dioxide, so that the micro-organisms stored in the cultivation room are inhibited from production due to the depletion of oxygen and the increase of carbon dioxide, and self-dissolving to form another sterilization, relative to the mycelium. The carbon dioxide resistance for anaerobic fungi can continue to grow mycelium in the environment of carbon and nitrogen sources (ratio), so that the cultivation of a hyphae in the incubation room (11) can be promoted into an excellent mycelium and promote the induction of mites. Ganoderma lucidum The ecological zone (as shown in the second figure); Step (7) harvest: After a predetermined incubation time, the fine hyphae inoculated with the culture dish and the bristles of the burdock are symbiotic bacteria, so that they can effectively produce good bacteria. Breeding with natural bacteria, transgenic bacteria of rot, burdock, burdock, and burdock, so that the burdock wood (20) has the mushroom body of the scorpion scorpion formed on the surface (as shown in the third figure) Show).

In this way, we will develop a method for inoculation and cultivation of burdock mushroom with high quality yield, neat finished product and effective simplification of cultivation process and equipment, so as to reduce the expenditure of various labor costs and large equipment costs.

In summary, it is known that the present invention can be widely utilized by related industries. It is extremely progressive and novel, and the invention has not been disclosed before the application, has complied with the provisions of the Patent Law, and has filed an invention patent application in accordance with the law.

The above is only the preferred embodiment of the present invention, and the scope of the present invention is not limited thereto; that is, the equivalent changes and modifications made by the scope of the present invention should still belong to the present invention. Within the scope of the patent.

10‧‧‧Cultivation barrel

11‧‧‧Cultivation room

12‧‧ ‧ high block

121‧‧‧ cushion

13‧‧‧Transparent hood

14‧‧‧ barrel lid

20‧‧‧ paragraph timber

30‧‧‧ water

40‧‧‧ cattle extract

The first figure is a flow chart of the method of the present invention.

The second figure is a schematic cross-sectional view of the state in the cultivation of the present invention.

The third figure is a schematic plan view of the burdock mushroom which is cultivated in the present invention.

10‧‧‧Cultivation barrel

11‧‧‧Cultivation room

12‧‧ ‧ high block

121‧‧‧ cushion

13‧‧‧Transparent hood

14‧‧‧ barrel lid

20‧‧‧ paragraph timber

30‧‧‧ water

Claims (2)

A method for inoculation and cultivation of Antrodia camphorata, which is prepared according to the appropriate amount of material (material), and has a cultivation barrel, an appropriate amount of burdock wood, water and burdock essential oil, and performs pretreatment of the first stage of the ash layer of the burdock wood. In the process, the first procedure is to polish the skin. The skin layer of the burdock wood is first polished to remove the surface bacteria, so that the capillary holes of the wood are opened; that is, the second cleaning process is carried out with water rinse, followed by a second cleaning procedure. The burdock essential oil is directly sprayed on the burdock wood for the third sterilization process, so that the burdock essential oil can effectively remove the bacteria and preserve the original strain of the burdock, thereby achieving the target effect of sterilization and reaching the diverse bacteria in the culture barrel ( Burdock) promotes the activity of bio-competitive instinct and activated strains, so that it can accelerate the growth and mass production of secondary products (B. chinensis); and then carry out the fourth high-temperature sterilization process after sterilizing the burdock wood. The hyphae of the burdock culture dish is transferred to the surface of the section of wood, that is, the pretreatment process of the ash layer of the burdock section of the fifth culture dish inoculation procedure is completed, and then the The cultivation step is carried out in a transposition culture tank. The cultivation steps are as follows: Step (1) Washing the barrel: Wash the cultivation barrel with water, and arrange a high-profile hollow tube-shaped cushion at the bottom of the barrel. And the initial spray sterilization is carried out on the inner surface of the barrel with burdock essential oil; Step (2) into the water: pour the appropriate volume ratio of water into the cultivation barrel, so that the water level in the barrel is lower than the height of the elevated block. Let the high block stretch out on the water for a suitable period, then take the burdock essential oil and spray it again on the inner surface of the barrel; Step (3) water sterilization: Take the predetermined volume ratio of the burdock essential oil into the cultivation barrel. At this time, the essential oil will float on the water surface and form the water source in the barrel for sterilization. The cultivation chamber will form a sterile room in the cultivation tank; The mycelium grows and simultaneously provides the bacteria in the water; Step (4) puts the wood into the section: the predetermined volume is compared with the post-implanted burdock section, and the stacking is placed in the cultivation tank. The block is arranged on the cushion layer; Step (5) closes the cultivation barrel: the barrel mouth of the cultivation barrel is covered with a transparent mold cover and covered by a bucket cover, so that the internal cultivation chamber forms a closed space, so that the section wood is quiet. The setting is set in the incubation room; Step (6) is statically cultured: during the cultivation, the water vapor is humidified by the moisture of the moisture in the closed culture tank to form a water droplet, and then the transparent mold is placed above. Shooting the water bead down, according to this cycle, naturally forming a breeding bucket of artificial rainforest ecological circle, using the hyphae with anaerobicity and the fermentation of the wood xylem in the decomposition section naturally produces a trace amount of carbon and nitrogen sources (ratio) of biogas. Conducive to the growth of mycelium and the growth of fruiting bodies, and the cultivation of indoors The increase of carbon oxide can effectively inhibit the production of bacteria or autolysis, so as to form another sterilization; Step (7) harvest: after a predetermined incubation time, the cultured bacteria and burdock symbiosis cultivating excellent strains, The rot fungus, burdock rot fungus, and burdock bacteria are transformed into the surface of the burdock wood with the scorpion scorpion fruit body, which can be used to form a finished product of high quality and good finished product. . In this way, the efficiency of various labor costs and equipment installations can be effectively reduced by simplifying the cultivation process of the burdock mushroom. For example, in the method of inoculating and cultivating a kind of Antrodia camphorata described in the first paragraph of the patent application, the ratio of the capacity of the cultivation barrel, the appropriate amount of the burdock wood, the water and the burdock essential oil is set to 5:3: 0.2:0.05.