CN109258304A - A kind of production method and growth condition of hickory chick liquid culture bacteria - Google Patents
A kind of production method and growth condition of hickory chick liquid culture bacteria Download PDFInfo
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- CN109258304A CN109258304A CN201811159066.XA CN201811159066A CN109258304A CN 109258304 A CN109258304 A CN 109258304A CN 201811159066 A CN201811159066 A CN 201811159066A CN 109258304 A CN109258304 A CN 109258304A
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- hickory chick
- liquid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/30—Accessories for use before inoculation of spawn, e.g. sterilisers
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- Mycology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of production method and growth condition of hickory chick liquid culture bacteria, this method breaks the mode of traditional solid-solid, using the mode of Liquid-liquid, cooperate the formula of our liquid cultivation seed culture medium simultaneously: sucrose 1.0-2.0% being added in the distilled water of 1L, albumen powder 0.10-0.50%, yeast powder 0.20-0.30%, bean cake powder 0.6-0.7%, K2HPO40.05-0.10%, KH2PO40.05-0.20% and MgSO40.05-0.10%, pH 6.3-6.8 are cultivated 5-7 days in 23 DEG C of fermentors, can carry out crop field or hayashishita sprinkling inoculation.On the one hand the process of production liquid cultivation seed is simple, at low cost and bacterium germination is fast, improves the efficiency of cultivation hickory chick significantly；On the other hand hickory chick cultivation under woods has successfully been carried out using liquid cultivation seed, has been that new cultivation mode is found in the cultivation of hickory chick.
Description
Technical field
The present invention relates to the production method and growth condition of a kind of hickory chick liquid culture bacteria, belong to edible mashroom cultivating,
Technical field of cultivation.
Background technique
Hickory chick (Morchella spp.) delicious meat is tender and crisp, nutritive and medicinal value is high, is a kind of food of preciousness
Medicinal fungus, it is deep to be liked by various countries people, but wild toadstool yield is few, is not able to satisfy consumer demand, forest land is imitated wild
Cultivation is to improve yield, indoors artificial cultivation is overcome to be not easy to form the cultural method of fructification.In production, solid is usually utilized
Cultivar carries out inoculated and cultured, and the solid state cultivation kind amount needed is big and mycelia cell age difference is big, and the labour needed is also very
Greatly, it is difficult to meet in production the needs of to cultivar amount.Therefore, explore the culture medium production method of liquid cultivation seed, and by this
Technique Popularizing is applied in the pseudo-wild cultivating of hickory chick, is the effective way for capableing of large-scale production hickory chick.
Summary of the invention
It is an object of that present invention to provide the production methods and growth condition of a kind of hickory chick liquid culture bacteria.
To achieve the above object, present invention provide the technical scheme that a kind of production side of hickory chick liquid culture bacteria
Method, including the following steps:
It is activated on the plate of PDA culture medium step 1: morchella mother culture is transferred to one and is accommodated, makes morchella mother culture
Mycelia vitality restoration, then segmentation obtains hickory chick original seed PDA fungus block；
Step 2: the hickory chick original seed PDA fungus block is inoculated into the PDB fluid nutrient medium after sterilizing, in 20-25 DEG C of condition
Under, it is cultivated 5-7 days with shaking table culture case in dark place, obtains hickory chick level-one kind；
Step 3: the hickory chick level-one kind is inoculated into the fermentor for accommodating hickory chick liquid cultivation seed culture medium, connect
, by the fermentor after inoculation with 20-25 DEG C culture 5-7 days；The hickory chick liquid cultivation seed culture medium the preparation method comprises the following steps:
It is added the sucrose of its quality 1.0-2.0% to 1 liter of distilled water, the yeast powder of the albumen powder of 0.10-0.50%, 0.20-0.30%,
The bean cake powder of 0.6-0.7%, the K of 0.05-0.10%2HPO4, the KH of 0.05-0.20%2PO4With the MgSO of 0.05-0.10%4, mixing
It is made after adjusting pH value to 6.3-6.8, then sterilized, cooling step after uniformly；
Step 4: being concentrated the hickory chick liquid spawn that third step culture obtains to get hickory chick using vacuum filtration machine
Liquid culture bacteria.
Preferred technical solution are as follows: the condition of sterilizing are as follows: temperature is 121 DEG C, pressure 0.15Mpa, and sterilization time is
15min。
To achieve the above object, present invention provide the technical scheme that a kind of cultivation of hickory chick liquid culture bacteria is answered
With, hickory chick liquid culture bacteria is placed in 4 DEG C of incubator, transport sowing place, by hickory chick liquid culture bacteria
It is put into clean plastic water bucket, 9-11 times of quality of hickory chick liquid culture bacteria of commercially available drummed pure water, stirring is added
It is uniformly dispersed in pure water to strain, directly crop field to be seeded or hayashishita is sprayed with water pump.
Detailed description of the invention
Attached drawing 1 is hickory chick liquid cultivation seed cultivation under woods hickory chick situation.
Since above-mentioned technical proposal is used, the present invention has the advantage, that compared with prior art
It is long that the present invention for traditional hickory chick strain solid connects the period in solid mode, and complicated for operation at high cost and cell age is poor
The disadvantages of different big, is connect the production of hybrid seeds mode of liquid using liquid, and combines the formula of fluid nutrient medium, and a kind of hickory chick liquid is obtained
The production method of cultivar is simultaneously carried out in hickory chick cultivation production.
Specific embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book is understood other advantages and efficacy of the present invention easily.
Embodiment one: a kind of production method and growth condition of hickory chick liquid culture bacteria
Hickory chick liquid cultivation seed carries out hickory chick cultivation in hayashishita
(1) preparation of hickory chick original seed
Morchella mother culture in test tube is transferred on PDA plate and is activated, continues switching activation 3 times in this way, to parent species
Mycelia vitality restoration.
(2) preparation of hickory chick liquid level-one kind
100mLPDB fluid nutrient medium is added in the triangular flask of 250mL, is 121 DEG C in temperature, goes out under conditions of pressure 0.15Mpa
Bacterium 15min.
(3) it by after sterilization and cooling, access diameter is 6 pieces of fungus block of hickory chick original seed PDA after the activation of 1cm, is placed in
It cultivates 7 days the speed dark place of 23 DEG C of shaking table culture case 150rpm.
(4) culture medium prescription of hickory chick liquid cultivation seed is: the formula of fluid nutrient medium is: adding in the distilled water of 1L
Enter to distill the sucrose of water quality 2.0%, 0.5% albumen powder, 0.3% yeast powder, 0.7% bean cake powder, 0.1% K2HPO4,
0.2% KH2PO4With 0.10% MgSO4, pH 6.8 is being 121 DEG C in temperature, sterilizing under conditions of pressure 0.15Mpa
20min, it is cooling.
(5) step (4) are housed by what the level liquid kind of step (2) cultured hickory chick was inoculated into that sterilizing cool down
In the fermentor of hickory chick liquid cultivation seed.Then, the fermentor after inoculation is cultivated 7 days with 23 DEG C.
(6) after to the liquid cultivation seed culture of hickory chick, hickory chick liquid spawn is carried out using vacuum filtration machine
Concentration, the hickory chick strain after concentration is placed in 4 DEG C of incubator, is transported sowing place, liquid cultivation seed is put into clean
In net plastic water bucket, 10 times of commercially available drummed pure waters of quality of strain are added, stirring is uniformly dispersed in pure water to strain,
Sprinkling hickory chick liquid cultivation seed directly is carried out in hayashishita to be seeded with water pump.Attached drawing 1 is the elite stand of hickory chick liquid culture
Lower cultivation hickory chick situation, A-B: mycelium germination；C: former base is sprouted；D: fruit body development.
Embodiment two: a kind of production method and growth condition of hickory chick liquid culture bacteria
A kind of production method of hickory chick liquid culture bacteria, including the following steps:
It is activated on the plate of PDA culture medium step 1: morchella mother culture is transferred to one and is accommodated, makes morchella mother culture
Mycelia vitality restoration, then segmentation obtains hickory chick original seed PDA fungus block；
Step 2: the hickory chick original seed PDA fungus block is inoculated into the PDB fluid nutrient medium after sterilizing, at 20 °C, use
Shaking table culture case is cultivated 5 days in dark place, obtains hickory chick level-one kind；
Step 3: the hickory chick level-one kind is inoculated into the fermentor for accommodating hickory chick liquid cultivation seed culture medium, connect
, the fermentor after inoculation is cultivated 5 days with 20 DEG C；The hickory chick liquid cultivation seed culture medium the preparation method comprises the following steps: to 1 liter
Distilled water is added the sucrose of its quality 1.0%, 0.10% albumen powder, 0.20% yeast powder, 0.6% bean cake powder, 0.05%
K2HPO4, 0.05% KH2PO4With 0.05% MgSO4, after adjusting pH value to 6.3, then sterilized, cooling step after mixing
It is made；
Step 4: being concentrated the hickory chick liquid spawn that third step culture obtains to get hickory chick using vacuum filtration machine
Liquid culture bacteria.
Preferred embodiment are as follows: the condition of sterilizing are as follows: temperature is 121 DEG C, pressure 0.15Mpa, and sterilization time is
15min。
Hickory chick liquid culture bacteria, is placed in 4 DEG C of incubator by a kind of growth condition of hickory chick liquid culture bacteria
It is interior, sowing place is transported, hickory chick liquid culture bacteria is put into clean plastic water bucket, hickory chick liquid is added and plants
The commercially available drummed pure water of 9 times of strain quality of training, stirring is uniformly dispersed in pure water to strain, with water pump directly to be seeded
Crop field or hayashishita sprayed.
Embodiment three: a kind of production method and growth condition of hickory chick liquid culture bacteria
A kind of production method of hickory chick liquid culture bacteria, including the following steps:
It is activated on the plate of PDA culture medium step 1: morchella mother culture is transferred to one and is accommodated, makes morchella mother culture
Mycelia vitality restoration, then segmentation obtains hickory chick original seed PDA fungus block；
Step 2: the hickory chick original seed PDA fungus block is inoculated into the PDB fluid nutrient medium after sterilizing, under the conditions of 25 DEG C, use
Shaking table culture case is cultivated 6 days in dark place, obtains hickory chick level-one kind；
Step 3: the hickory chick level-one kind is inoculated into the fermentor for accommodating hickory chick liquid cultivation seed culture medium, connect
, the fermentor after inoculation is cultivated 6 days with 25 DEG C；The hickory chick liquid cultivation seed culture medium the preparation method comprises the following steps: to 1 liter
Distilled water is added the sucrose of its quality 1.5%, 0.3% albumen powder, 0.25% yeast powder, 0.65% bean cake powder, 0.07%
K2HPO4, 0.12% KH2PO4With 0.07% MgSO4, after adjusting pH value to 6.5, then sterilized, cooling step after mixing
It is made；
Step 4: being concentrated the hickory chick liquid spawn that third step culture obtains to get hickory chick using vacuum filtration machine
Liquid culture bacteria.
Preferred embodiment are as follows: the condition of sterilizing are as follows: temperature is 121 DEG C, pressure 0.15Mpa, and sterilization time is
15min。
Hickory chick liquid culture bacteria, is placed in 4 DEG C of incubator by a kind of growth condition of hickory chick liquid culture bacteria
It is interior, sowing place is transported, hickory chick liquid culture bacteria is put into clean plastic water bucket, hickory chick liquid is added and plants
The commercially available drummed pure water of 11 times of strain quality of training, stirring is uniformly dispersed in pure water to strain, with water pump directly to waiting
It is sprayed in the crop field of kind.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (3)
1. a kind of production method of hickory chick liquid culture bacteria, it is characterised in that: include the following steps:
It is activated on the plate of PDA culture medium step 1: morchella mother culture is transferred to one and is accommodated, makes morchella mother culture
Mycelia vitality restoration, then segmentation obtains hickory chick original seed PDA fungus block；
Step 2: the hickory chick original seed PDA fungus block is inoculated into the PDB fluid nutrient medium after sterilizing, in 20-25 DEG C of condition
Under, it is cultivated 5-7 days with shaking table culture case in dark place, obtains hickory chick level-one kind；
Step 3: the hickory chick level-one kind is inoculated into the fermentor for accommodating hickory chick liquid cultivation seed culture medium, connect
, by the fermentor after inoculation with 20-25 DEG C culture 5-7 days；The hickory chick liquid cultivation seed culture medium the preparation method comprises the following steps:
It is added the sucrose of its quality 1.0-2.0% to 1 liter of distilled water, the yeast powder of the albumen powder of 0.10-0.50%, 0.20-0.30%,
The bean cake powder of 0.6-0.7%, the K of 0.05-0.10%2HPO4, the KH of 0.05-0.20%2PO4With the MgSO of 0.05-0.10%4, mixing
It is made after adjusting pH value to 6.3-6.8, then sterilized, cooling step after uniformly；
Step 4: being concentrated the hickory chick liquid spawn that third step culture obtains to get hickory chick using vacuum filtration machine
Liquid culture bacteria.
2. the production method of hickory chick liquid culture bacteria according to claim 1, it is characterised in that: the condition of sterilizing
Are as follows: temperature is 121 DEG C, pressure 0.15Mpa, sterilization time 15min.
3. a kind of growth condition of hickory chick liquid culture bacteria, it is characterised in that: hickory chick liquid culture bacteria is placed in 4 DEG C
Incubator in, transport sowing place, hickory chick liquid culture bacteria be put into clean plastic water bucket, sheep tripe is added
9-11 times of quality of bacteria liquid cultivation strain of commercially available drummed pure water, stirring are uniformly dispersed in pure water to strain, use water pump
Directly crop field to be seeded or hayashishita are sprayed.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111527820A (en) * | 2020-06-15 | 2020-08-14 | 上海市农业科学院 | Method for improving secondary salinized soil of vegetable greenhouse by using morchella cultivation |
| CN112772295A (en) * | 2021-01-29 | 2021-05-11 | 贵州省生物研究所 | Preparation method of morchella liquid strain and improved cultivar |
| CN115039635A (en) * | 2022-06-08 | 2022-09-13 | 甘肃河州云菇农业科技有限公司 | Liquid culture medium for preparing morchella stock culture in high altitude area and stock culture preparation method |
| CN115885770A (en) * | 2022-11-23 | 2023-04-04 | 黑龙江省科学院微生物研究所 | Morchella seeding method |
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| CN107513500A (en) * | 2016-06-17 | 2017-12-26 | 云南清湖山色农业科技有限公司 | A kind of preparation method of hickory chick liquid spawn |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111527820A (en) * | 2020-06-15 | 2020-08-14 | 上海市农业科学院 | Method for improving secondary salinized soil of vegetable greenhouse by using morchella cultivation |
| CN112772295A (en) * | 2021-01-29 | 2021-05-11 | 贵州省生物研究所 | Preparation method of morchella liquid strain and improved cultivar |
| CN115039635A (en) * | 2022-06-08 | 2022-09-13 | 甘肃河州云菇农业科技有限公司 | Liquid culture medium for preparing morchella stock culture in high altitude area and stock culture preparation method |
| CN115885770A (en) * | 2022-11-23 | 2023-04-04 | 黑龙江省科学院微生物研究所 | Morchella seeding method |
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Application publication date: 20190125 |