CN105831023A - Cultivating method of dolichocybidae - Google Patents
Cultivating method of dolichocybidae Download PDFInfo
- Publication number
- CN105831023A CN105831023A CN201610412847.XA CN201610412847A CN105831023A CN 105831023 A CN105831023 A CN 105831023A CN 201610412847 A CN201610412847 A CN 201610412847A CN 105831023 A CN105831023 A CN 105831023A
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- CN
- China
- Prior art keywords
- mite
- long head
- breeding method
- head mite
- dolichocybidae
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Abstract
The invention relates to a cultivating method of dolichocybidae. The cultivating method comprises the following steps of innoculating edible fungi mycelium into a culture medium of PDA (potato dextrose agar), overgrowing the edible fungi mycelium, then cutting a small circle with the diameter of 1cm in the middle part of the culture medium with the overgrown edible fungi mycelium, innoculating 1 to 30 female dolichocybidae adults into the small circle, controlling the humidity of a cultivating container to 50-95%, the outside temperature range of the cultivating container to 18-28 DEG C, and the relative humidity to 40-95%, and culturing for 7-30 days, so as to cultivate the dolichocybidae. The cultivating method has the advantages that (1) the pollution possibility of other fungi and bacteria is decreased; (2) the population of the dolichocybidae is favorably established; (3) the dolichocybidae is obtained from the white edible fungi mycelium, the body is white, and the small circle is cut in the middle part of the cultivating medium for conveniently observing. The cultivating method is suitable for cultivating the dolichocybidae eating edible fungi mycelium, such as dolichocybe perniciosa and dolichocybe shenagarici.
Description
Technical field
The invention belongs to breeding method field, be specifically related to the breeding method of a kind of long head mite.
Technical background
Chang Tou mite section (Dolichocybidae) long head mite genus (Dolichocybe) mite class is the edible mushroom great mite class that causes harm, its feeding habits are complicated, not only take food mycelia and the fructification of mushroom, asparagus, clitocybe maxima, black fungus, Uricularia polytricha, white fungus, glossy ganoderma etc., but also take food and propagate the miscellaneous bacterias such as wood is mould, nigrospora, sickle-like bacteria.
Long head mite is caused harm through the whole cultivation period of edible mushroom, cultigen of not only causing harm, and also can infect female kind and original seed.The biggest generation, almost feels simply helpless to it, it cause harm the production of hybrid seeds to edible mushroom and cultivation brings destructive disaster.Such as, in February, 2008, ZhangZhou mushroom agriculture finds that this mite is caused harm seriously in pork tripe mushroom bag, and clitocybe maxima cultivation loss in full village, more than 20,000,000 yuan, is almost had no harvest.2013-2014, Fujian asparagus factory female plants, long head mite all detected in original seed and cultigen, the said firm this time owing to being caused harm by this mite, economic loss at least 80 ten thousand yuan.
Edible fungus culturing is constituted a serious threat by long head mite, and fundamental biological knowledge characteristic and the Control Technology etc. of carrying out this mite are studied extremely urgent.In conjunction with producing reality, put the reason that this mite burst occurs in order, there is major application promotional value.
The most domestic breeding method being not yet good at a mite.The present invention relates to the breeding method of long head mite, it is intended to the series of studies such as fundamental biological knowledge for long head mite provides sufficient worm source, it is ensured that research is smoothed out.
Summary of the invention
It is an object of the invention to provide the breeding method of a kind of long head mite, utilize hypha of edible fungus to cultivate long head mite, the body colour of long head mite is white, and this method is easy to observational study.
For achieving the above object, the present invention adopts the following technical scheme that
The breeding method of a kind of long head mite: first hypha of edible fungus is accessed on the little culture dish equipped with sterilizing potato dextrose agar, treat that mycelia is covered with, covering with the roundlet cutting a diameter 1cm in the middle of the PDA culture medium of mycelia, then 1-30 the longest head mite female one-tenth mite is accessed in roundlet, cultivate container interior humidity and control between 50-95%, cultivation container ambient temperature scope is at 18-28 DEG C, and relative humidity controls at 40-95%, cultivates 7-30 days.
Described edible mushroom includes the one in asparagus, black fungus, Uricularia polytricha, white fungus, mushroom, glossy ganoderma and clitocybe maxima.
Described long head mite includes the one in the long head mite of evil and Shen mushroom long head mite.
Described PDA nutrient media components includes potato, glucose and agar, the mass ratio 10:1:1 of three.
Described PDA nutrient media components also includes peptone, cellulose.
Described little culture dish is the plastics batch cultur ware of a diameter of 3-6 cm.
The roundlet of described diameter 1cm refers to the roundlet that ring wall is 30-45 degree inclined-plane.
More excellent breeding condition is: cultivating container interior humidity and control at 50-80%, container ambient temperature scope is at 22-27 DEG C, and relative humidity controls at 50-85%, cultivates 10-20 days.
Use above-mentioned steps to cultivate to have the advantage that
(1) reduce by other fungi and the pollution probability of bacterium；(2) long head mite is conducive to set up population；(3) long head mite takes food white hypha of edible fungus, and body colour is white, and the method is easy to observe；(4) present invention may utilize hypha of edible fungus and cultivates long head mite, reaches long head mite conservation and provides the purpose in worm source for series of studies such as long head mite biology.
Detailed description of the invention
Following methods is only the citing of the embodiment to claim and summary of the invention, does not represent the restriction to present claims and summary of the invention.
Embodiment
1
Flammulina velutipes mycelium is accessed on the little culture dish equipped with sterilizing potato dextrose agar, it is placed in 25 DEG C of incubators to cultivate 7 days, treat that mycelia is covered with, to cover with the roundlet cutting a diameter 1cm in the middle of the culture medium of mycelia, and then access 1 long head mite female one-tenth mite in roundlet, little culture dish interior humidity controls at 60-65%, cultivate container ambient temperature scope at 22-25 DEG C, relative humidity controls at 80-85%, cultivates 15 days, i.e. observes long head mite population growth.
Embodiment
2
Black fungus bacterial wire connection is entered on the little culture dish equipped with sterilizing potato dextrose agar, it is placed in 22 DEG C of incubators to cultivate 10 days, treat that mycelia is covered with, to cover with the roundlet cutting a diameter 1cm in the middle of the culture medium of mycelia, and then access 20 long head mite female one-tenth mites in roundlet, little culture dish interior humidity controls at 50-55%, cultivate container ambient temperature scope at 22-25 DEG C, relative humidity controls at 75-80%, cultivates 10 days, i.e. observes long head mite population growth.
Embodiment
3
Mushroom mycelium is accessed on the little culture dish equipped with sterilizing potato dextrose agar, it is placed in 24 DEG C of incubators to cultivate 8 days, treat that mycelia is covered with, to cover with the roundlet cutting a diameter 1cm in the middle of the culture medium of mycelia, and then access 20 long head mite female one-tenth mites in roundlet, little culture dish interior humidity controls at 50-55%, cultivate container ambient temperature scope at 22-25 DEG C, relative humidity controls at 75-80%, cultivates 10 days, i.e. observes long head mite population growth.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent and modification, all should belong to the covering scope of the present invention.
Claims (8)
1. the breeding method of one kind long head mite, it is characterized in that: first white hypha of edible fungus is accessed on the little culture dish equipped with sterilizing potato dextrose agar, treat that mycelia is covered with, covering with the roundlet cutting a diameter 1cm in the middle of the PDA culture medium of mycelia, then access 1-30 the longest head mite female one-tenth mite in roundlet, cultivate container interior humidity and control between 50-95%, cultivate container ambient temperature scope at 18-28 DEG C, relative humidity controls at 40-95%, cultivates 7-30 days.
The breeding method of long head mite the most according to claim 1, it is characterised in that: described edible mushroom includes the one in asparagus, black fungus, Uricularia polytricha, white fungus, mushroom, glossy ganoderma and clitocybe maxima.
3. according to the breeding method of the long head mite described in claim 1, it is characterised in that: described long head mite includes the one in the long head mite of evil and Shen mushroom long head mite.
The breeding method of long head mite the most according to claim 1, it is characterised in that: described PDA nutrient media components includes potato, glucose and agar, the mass ratio 10:1:1 of three.
The breeding method of long head mite the most according to claim 4, it is characterised in that: described PDA nutrient media components also includes peptone, cellulose.
The breeding method of long head mite the most according to claim 1, it is characterised in that: described little culture dish is the plastics batch cultur ware of a diameter of 3-6 cm.
The breeding method of long head mite the most according to claim 1, it is characterised in that: the roundlet of described diameter 1cm refers to the roundlet that ring wall is 30-45 degree inclined-plane.
Long head mite breeding method the most according to claim 1, it is characterised in that: cultivating container interior humidity and control at 50-80%, container ambient temperature scope is at 22-27 DEG C, and relative humidity controls at 50-85%, cultivates 10-20 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610412847.XA CN105831023A (en) | 2016-06-14 | 2016-06-14 | Cultivating method of dolichocybidae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610412847.XA CN105831023A (en) | 2016-06-14 | 2016-06-14 | Cultivating method of dolichocybidae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105831023A true CN105831023A (en) | 2016-08-10 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610412847.XA Pending CN105831023A (en) | 2016-06-14 | 2016-06-14 | Cultivating method of dolichocybidae |
Country Status (1)
| Country | Link |
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| CN (1) | CN105831023A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107027712A (en) * | 2017-04-07 | 2017-08-11 | 合肥天敌之家生物技术有限公司 | A kind of colouring method of tyrophagus putrescentiae digestive system |
| CN107494625A (en) * | 2017-09-26 | 2017-12-22 | 江苏菇本堂生物科技股份有限公司 | A kind of acarid for planting edible mushroom detects liquid |
Citations (5)
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|---|---|---|---|---|
| EP1236394A1 (en) * | 2001-03-01 | 2002-09-04 | Stallergenes S.A. | Method for rearing house dust mites, preparation of the culture for such method and preparation of allergenic extracts from such mites |
| CN101331869A (en) * | 2008-08-07 | 2008-12-31 | 北京市农林科学院 | Chile phytoseiulus persimilis propagating method |
| CN101406167A (en) * | 2008-09-25 | 2009-04-15 | 福建农林大学 | Method for considerably feeding and using ascidae |
| CN102657136A (en) * | 2012-01-17 | 2012-09-12 | 江苏省农业科学院 | Indoor mass breeding method of edible fungus and mite in glove box |
| CN103181368A (en) * | 2012-09-18 | 2013-07-03 | 上海雪榕生物科技股份有限公司 | Method for detecting and controlling acarid during fungus mushroom cultivation |
-
2016
- 2016-06-14 CN CN201610412847.XA patent/CN105831023A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1236394A1 (en) * | 2001-03-01 | 2002-09-04 | Stallergenes S.A. | Method for rearing house dust mites, preparation of the culture for such method and preparation of allergenic extracts from such mites |
| CN101331869A (en) * | 2008-08-07 | 2008-12-31 | 北京市农林科学院 | Chile phytoseiulus persimilis propagating method |
| CN101406167A (en) * | 2008-09-25 | 2009-04-15 | 福建农林大学 | Method for considerably feeding and using ascidae |
| CN102657136A (en) * | 2012-01-17 | 2012-09-12 | 江苏省农业科学院 | Indoor mass breeding method of edible fungus and mite in glove box |
| CN103181368A (en) * | 2012-09-18 | 2013-07-03 | 上海雪榕生物科技股份有限公司 | Method for detecting and controlling acarid during fungus mushroom cultivation |
Non-Patent Citations (1)
| Title |
|---|
| 韩日畴等: "昆虫病原线虫一级种单菌培养的一种方法", 《昆虫天敌》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107027712A (en) * | 2017-04-07 | 2017-08-11 | 合肥天敌之家生物技术有限公司 | A kind of colouring method of tyrophagus putrescentiae digestive system |
| CN107494625A (en) * | 2017-09-26 | 2017-12-22 | 江苏菇本堂生物科技股份有限公司 | A kind of acarid for planting edible mushroom detects liquid |
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Application publication date: 20160810 |
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